Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| ultra-deep next generation mitochondrial genome sequencing reveals widespread heteroplasmy in chinese hamster ovary cells. | recent sequencing of the chinese hamster ovary (cho) cell and chinese hamster genomes has dramatically advanced our ability to understand the biology of these mammalian cell factories. in this study, we focus on the powerhouse of the cho cell, the mitochondrion. utilizing a high-resolution next generation sequencing approach we sequenced the chinese hamster mitochondrial genome for the first time and surveyed the mutational landscape of cho cell mitochondrial dna (mtdna). depths of coverage rang ... | 2017 | 28188893 |
| fatty-acyl chain profiles of cellular phosphoinositides. | phosphoinositides are rapidly turning-over phospholipids that play key roles in intracellular signaling and modulation of membrane effectors. through technical refinements we have improved sensitivity in the analysis of the phosphoinositide pi, pip, and pip2 pools from living cells using mass spectrometry. this has permitted further resolution in phosphoinositide lipidomics from cell cultures and small samples of tissue. the technique includes butanol extraction, derivatization of the lipids, po ... | 2017 | 28189644 |
| magnetic sorting of membrane associated igg for phenotype-based selection of stable antibody producing cells. | to establish a simple and widely accessible technique for rapidly selecting high producing chinese hamster ovary (cho) cells engineered to express a monoclonal antibody (mab), we have exploited the transient display of recombinant protein on their cell surface. in combination with magnetic bead-based methods, we demonstrate the ability to select for cells of high productivity in the absence of any metabolic-based selection method. this technique is sufficient to obtain genetically stable enginee ... | 2017 | 28189705 |
| multiple blood-brain barrier transport mechanisms limit bumetanide accumulation, and therapeutic potential, in the mammalian brain. | there is accumulating evidence that bumetanide, which has been used over decades as a potent loop diuretic, also exerts effects on brain disorders, including autism, neonatal seizures, and epilepsy, which are not related to its effects on the kidney but rather mediated by inhibition of the neuronal na-k-cl cotransporter isoform nkcc1. however, following systemic administration, brain levels of bumetanide are typically below those needed to inhibit nkcc1, which critically limits its clinical use ... | 2017 | 28192112 |
| identification of human junctional adhesion molecule 1 as a functional receptor for the hom-1 calicivirus on human cells. | the hom-1 vesivirus was reported in 1998 following the inadvertent transmission of the animal calicivirus san miguel sea lion virus to a human host in a laboratory. we characterized the hom-1 strain and investigated the mechanism by which human cells could be infected. an expression library of 3,559 human plasma membrane proteins was screened for reactivity with hom-1 virus-like particles, and a single interacting protein, human junctional adhesion molecule 1 (hjam1), was identified. transient e ... | 2017 | 28196955 |
| distinct c-mannosylation of netrin receptor thrombospondin type 1 repeats by mammalian dpy19l1 and dpy19l3. | thrombospondin type 1 repeats (tsrs) occur in diverse proteins involved in adhesion and signaling. the two extracellular tsrs of the netrin receptor unc5a contain wxxwxxwxxc motifs that can be c-mannosylated on all tryptophans. a single c-mannosyltransferase (dumpy-19, dpy-19), modifying the first two tryptophans, occurs in caenorhabditis elegans, but four putative enzymes (dpy-19-like 1-4, dpy19l1-4) exist in mammals. single and triple crispr-cas9 knockouts of the three homologs that are expres ... | 2017 | 28202721 |
| antioxidant and cytoprotective effects of morin against hydrogen peroxide-induced oxidative stress are associated with the induction of nrf-2‑mediated ho-1 expression in v79-4 chinese hamster lung fibroblasts. | natural phytochemicals of plant origin, including flavonoids, have been found to be potent antioxidants providing beneficial effects against oxidative stress-related diseases. the present study was carried out to investigate the antioxidant properties of morin, a flavonoid originally isolated from the flowering plants of the moraceae family. superoxide dismutase (sod)‑like activity and 2,2'‑azino‑bis‑(3‑ethylbenzothiazoline‑6‑sulfonic acid) (abts•+) radical scavenging activity were determined. w ... | 2017 | 28204816 |
| manipulating cholesterol status within cells. | cellular cholesterol levels are intricately controlled to maintain homeostasis. here, we describe ways in which cellular cholesterol status can be manipulated for the study of cholesterol homeostasis, including sterol starvation (by culturing cells in lipoprotein-deficient serum and pretreating/treating with the cholesterol-lowering drug, statin) and sterol enrichment (using cholesterol complexed to cyclodextrin, and low-density lipoprotein). we also describe how to prepare lipoprotein-deficient ... | 2017 | 28205165 |
| high throughput discovery of new fouling-resistant surfaces. | a novel high throughput method for synthesis and screening of customized protein-resistant surfaces was developed. this method is an inexpensive, fast, reproducible and scalable approach to synthesize and screen protein-resistant surfaces appropriate for a specific feed. the method is illustrated here by combining a high throughput platform (htp) approach together with our patented photo-induced graft polymerization (pgp) method developed for facile modification of commercial poly(aryl sulfone) ... | 2011 | 28210070 |
| a scalable lysyl hydroxylase 2 expression system and luciferase-based enzymatic activity assay. | hydroxylysine aldehyde-derived collagen cross-links (hlccs) accumulate in fibrotic tissues and certain types of cancer and are thought to drive the progression of these diseases. hlcc formation is initiated by lysyl hydroxylase 2 (lh2), an fe(ii) and α-ketoglutarate (αkg)-dependent oxygenase that hydroxylates telopeptidyl lysine residues on collagen. development of lh2 antagonists for the treatment of these diseases will require a reliable source of recombinant lh2 protein and a non-radioactive ... | 2017 | 28216326 |
| matrix attachment region combinations increase transgene expression in transfected chinese hamster ovary cells. | matrix attachment regions (mars) are cis-acting dna elements that can increase transgene expression levels in a cho cell expression system. to investigate the effects of mar combinations on transgene expression and the underlying regulatory mechanisms, we generated constructs in which the enhanced green fluorescent protein (egfp) gene flanked by different combinations of human β-interferon and β-globin mar (imar and gmar, respectively), which was driven by the cytomegalovirus (cmv) or simian vir ... | 2017 | 28216629 |
| unveiling gene trait relationship by cross-platform meta-analysis on chinese hamster ovary cell transcriptome. | in the past few years, transcriptome analysis has been increasingly employed to better understand the physiology of chinese hamster ovary (cho) cells at a global level. as more transcriptome data accumulated, meta-analysis on data sets collected from various sources can potentially provide better insights on common properties of those cells. here, we performed meta-analysis on transcriptome data of different cho cell lines obtained using nimblegen or affymetrix microarray platforms. hierarchical ... | 2017 | 28218403 |
| glycoengineering of esterase activity through metabolic flux-based modulation of sialic acid. | this report describes the metabolic glycoengineering (mge) of intracellular esterase activity in human colon cancer (ls174t) and chinese hamster ovary (cho) cells. in silico analysis of the carboxylesterases ces1 and ces2 suggested that these enzymes are modified with sialylated n-glycans, which are proposed to stabilize the active multimeric forms of these enzymes. this premise was supported by treating cells with butanolylated mannac to increase sialylation, which in turn increased esterase ac ... | 2017 | 28218815 |
| interaction of approved drugs with synaptic vesicle protein 2a. | levetiracetam (lev) and its recently approved derivative brivaracetam are anti-epileptic drugs with a unique mechanism of action. the synaptic vesicle protein 2a (sv2a) was previously identified as their main target. in the current study, we tested a collection of 500 approved drugs for interaction with the human sv2a protein expressed in chinese hamster ovary cells. competition binding studies were performed using cell lysates with high sv2a expression and [(3) h]brivaracetam as a radioligand. ... | 2017 | 28220535 |
| how important is thermodynamics for identifying elementary flux modes? | we present a method for computing thermodynamically feasible elementary flux modes (tefms) using equilibrium constants without need of internal metabolite concentrations. the method is compared with the method based on a binary distinction between reversible and irreversible reactions. when all reactions are reversible, adding the constraints based on equilibrium constants reduces the number of elementary flux modes (efms) by a factor of two. declaring in advance some reactions as irreversible, ... | 2017 | 28222104 |
| evaluating the expression profile and stability of different ucoe containing vector combinations in mab-producing cho cells. | as the demand for monoclonal antibodies (mab) increases, more efficient expression methods are required for their manufacturing process. transcriptional gene silencing is a common phenomenon in recombinant cell lines which leads to expression reduction and instability. there are reports on improved antibody expression in ubiquitous chromatin opening element (ucoe) containing both heavy and light chain gene constructs. here we investigate the impact of having these elements as part of the light c ... | 2017 | 28228095 |
| what can mathematical modelling say about cho metabolism and protein glycosylation? | chinese hamster ovary cells have been in the spotlight for process optimization in recent years, due to being the major, long established cell factory for the production of recombinant proteins. a deep, quantitative understanding of cho metabolism and mechanisms involved in protein glycosylation has proven to be attainable through the development of high throughput technologies. here we review the most notable accomplishments in the field of modelling cho metabolism and protein glycosylation. | 2017 | 28228925 |
| antiglycopeptide mouse monoclonal antibody lpmab-21 exerts antitumor activity against human podoplanin through antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity. | the interaction between podoplanin (pdpn) and c-type lectin-like receptor 2 (clec-2) is involved in tumor malignancy. we have established many monoclonal antibodies (mabs) against human podoplanin using the cancer-specific mab (casmab) technology. lpmab-21, one of the mouse antipodoplanin mabs, is of the igg2a subclass, and its minimum epitope was determined to be thr76-arg79 of the human podoplanin. importantly, sialic acid is linked to thr76; therefore, lpmab-21 is an antiglycopeptide mab (gpm ... | 2017 | 28234556 |
| an automated micro-total immunoassay system for measuring cancer-associated α2,3-linked sialyl n-glycan-carrying prostate-specific antigen may improve the accuracy of prostate cancer diagnosis. | the low specificity of the prostate-specific antigen (psa) for early detection of prostate cancer (pca) is a major issue worldwide. the aim of this study to examine whether the serum pca-associated α2,3-linked sialyl n-glycan-carrying psa (s2,3psa) ratio measured by automated micro-total immunoassay systems (μtas system) can be applied as a diagnostic marker of pca. the μtas system can utilize affinity-based separation involving noncovalent interaction between the immunocomplex of s2,3psa and ma ... | 2017 | 28241428 |
| antibody purification from cho cell supernatant using new multimodal membranes. | this contribution describes strategies to purify monoclonal antibodies from chinese hamster ovary (cho) cell culture supernatant using newly designed multimodal membranes (mmms). the mmms were used for the capture step purification of human igg1 following a size-exclusion desalting column to remove chaotropic salts that interfere with igg binding. the mmm column attained higher dynamic binding capacity than a protein a resin column at an equivalent residence time of 1 min. the two-step mmm chrom ... | 2017 | 28248007 |
| effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell protein release in chinese hamster ovary cells. | recombinant human acid alpha glucosidase (gaa) is the therapeutic enzyme used for the treatment of pompe disease, a rare genetic disorder characterized by gaa deficiency in the cell lysosomes (raben et al., curr mol med. 2002; 2:145-166). the manufacturing process for gaa can be challenging, in part due to protease degradation. the overall goal of this study was to understand the effects of gaa overexpression on cell lysosomal phenotype and host cell protein (hcp) release, and any resultant cons ... | 2017 | 28249362 |
| the enhancement of antibody concentration and achievement of high cell density cho cell cultivation by adding nucleoside. | recently, with the dramatic increase in demand for therapeutic antibodies, chinese hamster ovary (cho) cell culture systems have made significant progress in recombinant antibody production. over the past two decades, recombinant antibody productivity has been improved by more than 100-fold. medium optimization has been identified as an important key approach for increasing product concentrations. in this study, we evaluated the effects of deoxyuridine addition to fed-batch cultures of antibody- ... | 2017 | 28251404 |
| a systematic approach to time-series metabolite profiling and rna-seq analysis of chinese hamster ovary cell culture. | chinese hamster ovary (cho) cells are the primary host used for biopharmaceutical protein production. the engineering of cho cells to produce higher amounts of biopharmaceuticals has been highly dependent on empirical approaches, but recent high-throughput "omics" methods are changing the situation in a rational manner. omics data analyses using gene expression or metabolite profiling make it possible to identify key genes and metabolites in antibody production. systematic omics approaches using ... | 2017 | 28252038 |
| gold nanoparticles and electroporation impose both separate and synergistic radiosensitizing effects in ht-29 tumor cells: an in vitro study. | radiation therapy (rt) is the gold standard treatment for more than half of known tumors. despite recent improvements in rt efficiency, the side effects of ionizing radiation (ir) in normal tissues are a dose-limiting factor that restricts higher doses in tumor treatment. one approach to enhance the efficiency of rt is the application of radiosensitizers to selectively increase the dose at the tumor site. gold nanoparticles (gnps) and electroporation (ep) have shown good potential as radiosensit ... | 2017 | 28260889 |
| screening for small molecule inhibitors of statin-induced app c-terminal toxic fragment production. | alzheimer's disease (ad) is characterized by neuronal and synaptic loss. one process that could contribute to this loss is the intracellular caspase cleavage of the amyloid precursor protein (app) resulting in release of the toxic c-terminal 31-amino acid peptide app-c31 along with the production of appδc31, full-length app minus the c-terminal 31 amino acids. we previously found that a mutation in app that prevents this caspase cleavage ameliorated synaptic loss and cognitive impairment in a mu ... | 2017 | 28261092 |
| a role of corazonin receptor in larval-pupal transition and pupariation in the oriental fruit fly bactrocera dorsalis (hendel) (diptera: tephritidae). | corazonin (crz) is a neuropeptide hormone, but also a neuropeptide modulator that is internally released within the cns, and it has a widespread distribution in insects with diverse physiological functions. here, we identified and cloned the cdnas of bactrocera dorsalis that encode crz and its receptor crzr. mature bdcrz has 11 residues with a unique ser(11) substitution (instead of the typical asn) and a his in the evolutionary variable position 7. the bdcrzr cdna encodes a putative protein of ... | 2017 | 28261106 |
| mirna engineering of cho cells facilitates production of difficult-to-express proteins and increases success in cell line development. | in recent years, coherent with growing biologics portfolios also the number of complex and thus difficult-to-express (dte) therapeutic proteins has increased considerably. dte proteins challenge bioprocess development and can include various therapeutic protein formats such as monoclonal antibodies (mabs), multi-specific affinity scaffolds (e.g. bispecific antibodies), cytokines or fusion proteins. hence, the availability of robust and versatile chinese hamster ovary (cho) host cell factories is ... | 2017 | 28262952 |
| understanding of decreased sialylation of fc-fusion protein in hyperosmotic recombinant chinese hamster ovary cell culture: n-glycosylation gene expression and n-linked glycan antennary profile. | to understand the effects of hyperosmolality on protein glycosylation, recombinant chinese hamster ovary (rcho) cells producing the fc-fusion protein were cultivated in hyperosmolar medium resulting from adding nacl (415 mosm/kg). the hyperosmotic culture showed increased specific fc-fusion protein productivity (qfc ) but a decreased proportion of acidic isoforms and sialic acid content of the fc-fusion protein. the intracellular and extracellular sialidase activities in the hyperosmotic culture ... | 2017 | 28266015 |
| optimizing cell-free protein expression in cho: assessing small molecule mass transfer effects in various reactor configurations. | cell-free protein synthesis (cfps) is an ideal platform for rapid and convenient protein production. however, bioreactor design remains a critical consideration in optimizing protein expression. using turbo green fluorescent protein (tgfp) as a model, we tracked small molecule components in a chinese hamster ovary (cho) cfps system to optimize protein production. here, three bioreactors in continuous-exchange cell-free (cecf) format were characterized. a gfp optical sensor was built to monitor t ... | 2017 | 28266026 |
| antioxidant effect of thiazolidine molecules in cell culture media improves stability and performance. | the ability of cell culture media components to generate reactive species as well as their sensitivity to oxidative degradation, affects the overall stability of media and the behavior of cells cultured in vitro. this study investigates the influence of thiazolidine molecules, formed from the condensation between cysteine and alpha-ketoacids, on the stability of these complex mixtures and on the performance of cell culture processes aiming to produce therapeutically relevant monoclonal antibodie ... | 2017 | 28268250 |
| enzyme replacement therapy for farber disease: proof-of-concept studies in cells and mice. | a series of studies were carried out in farber disease (omim #228000) cells and mice to evaluate the feasibility of enzyme replacement therapy (ert) for this disorder. media from chinese hamster ovary (cho) cells overexpressing human recombinant acid ceramidase (rhac) was used to treat fibroblasts from a farber disease patient, leading to significantly reduced ceramide. we also found that chondrocytes from farber disease mice had a markedly abnormal chondrogenic phenotype, and this was corrected ... | 2017 | 28275553 |
| in search of acceptable alternatives to the murine histamine sensitisation test (hist): what is possible and practical? | the 'international workshop on alternatives to the murine histamine sensitization test for acellular pertussis vaccines: in search of acceptable alternatives to the murine histamine sensitization test (hist): what is possible and practical?' was held on 4 and 5 march 2015 in london, united kingdom. participants discussed the results of the data generated from an international collaborative study (bsp114 phase 2) sponsored by the european directorate for the quality of medicines & health care (ed ... | 2016 | 28279256 |
| proteomic analysis of host cell protein dynamics in the culture supernatants of antibody-producing cho cells. | chinese hamster ovary (cho) cells are the most common cell line used for the production of therapeutic proteins including monoclonal antibodies (mabs). host cell proteins (hcps), secreted and released from lysed cells, accumulate extracellularly during the cultures of recombinant cho (rcho) cells, potentially impairing product quality. in an effort to maintain good mab quality during the cultures, hcps accumulated extracellularly in batch and fed-batch cultures of a mab-producing rcho cell line ... | 2017 | 28281648 |
| binding and biologic characterization of recombinant human serum albumin-etgfbr2 fusion protein expressed in cho cells. | transforming growth factor-β1 (tgf-β1) signaling is involved in cell metabolism, growth, differentiation, carcinoma invasion and fibrosis development, which suggests tgf-β1 can be treated as a therapeutic target extensively. because tgf-β1 receptor type α(tgfbr2) is the directed and essential mediator for tgf-β1 signals, the extracellular domain of tgfbr2 (etgfbr2), binding partner for tgf-β1, has been produced in a series of expression systems to inhibit tgf-β1 signaling. however, etgfbr2 is un ... | 2017 | 28281868 |
| efficient expression of stable recombinant human insulin-like growth factor-1 fusion with human serum albumin in chinese hamster ovary cells. | insulin-like growth factor-1 (igf-1) plays a crucial role in cell development, differentiation, and metabolism, and has been a potential therapeutic agent for many diseases. chinese hamster ovary (cho) cells are widely used for production of recombinant therapeutic proteins, but the expression level of igf-1 in cho cells is very low (1,500 µg/l) and the half-life of igf-1 in blood circulation is only 4.5 min according to previous studies. therefore, igf-1 was fused to long-circulating serum prot ... | 2017 | 28281882 |
| cytotoxicities and genotoxicities of cements based on calcium silicate and of dental formocresol. | increasing interest is being paid to the toxicities of dental materials. the purpose of this study was to determine the cytotoxicities and genotoxicities of endodontic compounds to chinese hamster ovary (cho-k1) reproductive cells. cultured cho-k1 cells were treated with dental formocresol, two types of calcium hydroxide paste, and two types of mineral trioxide aggregate cement for 24h. a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was performed on each culture, and the m ... | 2017 | 28283090 |
| seroprevalence for 2117-like vesiviruses in italian household dogs. | in 2003, a novel calicivirus, the vesivirus (vev) strain 2117, was identified incidentally as a contaminant in chinese hamster ovary (cho) cell cultures by a german pharmaceutical company. similar contaminations have been documented in three additional episodes, in bio-reactors used for production of recombinant drugs. more, recently 2117-like vevs have also been identified at high prevalence in the stools from asymptomatic kennel dogs and only sporadically in diarrhoeic and healthy household do ... | 2017 | 28284600 |
| a semi-empirical glycosylation model of a camelid monoclonal antibody under hypothermia cell culture conditions. | the impact of cell culture environment on the glycan distribution of a monoclonal antibody (mab) has been investigated through a combination of experiments and modeling. a newly developed cho duxb cell line was cultivated at two levels of initial glutamine (gln) concentrations (0, 4 mm) and incubation temperatures of (33 and 37 °c) in batch operation mode. hypothermia was applied either through the entire culture duration or only during the post-exponential phase. beyond reducing cell growth and ... | 2017 | 28285402 |
| high glucose enhances camp level and extracellular signal-regulated kinase phosphorylation in chinese hamster ovary cell: usage of br-camp in foreign protein β-galactosidase expression. | glucose is a carbon source for chinese hamster ovary (cho) cell growth, while low growth rate is considered to enhance the production of recombinant proteins. the present study reveals that glucose concentrations higher than 1 g/l reduce the growth rate and substantially increase in camp (∼300%) at a high glucose concentration (10 g/l). high glucose also enhances the phosphorylation of extracellular signal-regulated kinase (erk) and p27(kip) by western blot analysis. to determine whether the pho ... | 2017 | 28286121 |
| multiple expression cassette exchange via tp901-1, r4, and bxb1 integrase systems on a mouse artificial chromosome. | the site-specific excision of a target dna sequence for genetic knockout or lineage tracing is a powerful tool for investigating biological systems. currently, site-specific recombinases (ssrs), such as cre or flp recombination target cassettes, have been successfully excised or inverted by a single ssr to regulate transgene expression. however, the use of a single ssr might restrict the complex control of gene expression. this study investigated the potential for expanding the multiple regulati ... | 2017 | 28286726 |
| dielectrophoresis study of temporal change in internal conductivity of single cho cells after electroporation by pulsed electric fields. | applying sufficiently strong pulsed electric fields to a cell can permeabilize the membrane and subsequently affect its dielectric properties. in this study, we employ a microfluidic dielectrophoresis cytometry technique to simultaneously electroporate and measure the time-dependent dielectric response of single chinese hamster ovary cells. using experimental measurements along with numerical simulations, we present quantitative results for the changes in the cytoplasm conductivity of single cel ... | 2017 | 28289483 |
| copper(i)-phosphine polypyridyl complexes: synthesis, characterization, dna/hsa binding study, and antiproliferative activity. | a series of copper(i)-phosphine polypyridyl complexes have been investigated as potential antitumor agents. the complexes [cu(pph3)2dpq]no3 (2), [cu(pph3)2dppz]no3 (3), [cu(pph3)2dppa]no3 (4), and [cu(pph3)2dppme]no3 (5) were synthesized by the reaction of [cu(pph3)2no3] with the respective planar ligand under mild conditions. these copper complexes were fully characterized by elemental analysis, molar conductivity, fab-ms, and nmr, uv-vis, and ir spectroscopies. interactions between these coppe ... | 2017 | 28290674 |
| a novel sugar analog enhances sialic acid production and biotherapeutic sialylation in cho cells. | a desirable feature of many therapeutic glycoprotein production processes is to maximize the final sialic acid content. in this study, the effect of applying a novel chemical analog of the sialic acid precursor n-acetylmannosamine (mannac) on the sialic acid content of cellular proteins and a model recombinant glycoprotein, erythropoietin (epo), was investigated in cho-k1 cells. by introducing the 1,3,4-o-bu3 mannac analog at 200-300 µm into cell culture media, the intracellular sialic acid cont ... | 2017 | 28295160 |
| evaluation of two public genome references for chinese hamster ovary cells in the context of rna-seq based gene expression analysis. | rna-seq is a powerful transcriptomics tool for mammalian cell culture process development. successful rna-seq data analysis requires a high quality reference for read mapping and gene expression quantification. currently, there are two public genome references for chinese hamster ovary (cho) cells, the predominant mammalian cell line in the biopharmaceutical industry. in this study, we compared these two references by analyzing 60 rna-seq samples from a variety of cho cell culture conditions. am ... | 2017 | 28295162 |
| network reconstruction of the mouse secretory pathway applied on cho cell transcriptome data. | protein secretion is one of the most important processes in eukaryotes. it is based on a highly complex machinery involving numerous proteins in several cellular compartments. the elucidation of the cell biology of the secretory machinery is of great importance, as it drives protein expression for biopharmaceutical industry, a 140 billion usd global market. however, the complexity of secretory process is difficult to describe using a simple reductionist approach, and therefore a promising avenue ... | 2017 | 28298216 |
| transcriptomic changes in cho cells after adaptation to suspension growth in protein-free medium analysed by a species-specific microarray. | chinese hamster ovary (cho) cells are the preferred cell line for production of biopharmaceuticals. these cells are capable to grow without serum supplementation, but drastic changes in their phenotype occur during adaptation to protein-free growth, which typically include the change to a suspension phenotype with reduced growth rate. a possible approach to understand this transformation, with the intention to counteract the reduction in growth by targeted supplementation of protein-free media, ... | 2017 | 28302587 |
| antitumor activity of chlpmab-2, a human-mouse chimeric cancer-specific antihuman podoplanin antibody, via antibody-dependent cellular cytotoxicity. | human podoplanin (hpdpn), a platelet aggregation-inducing transmembrane glycoprotein, is expressed in different types of tumors, and it binds to c-type lectin-like receptor 2 (clec-2). the overexpression of hpdpn is involved in invasion and metastasis. anti-hpdpn monoclonal antibodies (mabs) such as nz-1 have shown antitumor and antimetastatic activities by binding to the platelet aggregation-stimulating (plag) domain of hpdpn. recently, we developed a novel mouse anti-hpdpn mab, lpmab-2, using ... | 2017 | 28332312 |
| an aromatic amino acid within intracellular loop 2 of the prostaglandin ep2 receptor is a prerequisite for selective association and activation of gαs. | we previously demonstrated that the aromatic moiety of tyr(143) within the intracellular loop 2 (icl2) region of the prostaglandin ep2 receptor plays a crucial role in gs coupling. here we investigated whether the icl2 of the ep2 receptor directly binds to gαs and whether an aromatic moiety affects this interaction. in chinese hamster ovary cells, mutations of tyr(143) reduced the ability of the ep2 receptor to interact with g proteins as demonstrated by gtpγs sensitivity, as well as the ability ... | 2017 | 28336329 |
| studies to examine potential tolerability differences between the 5-ht2c receptor selective agonists lorcaserin and cp-809101. | lorcaserin (lor) is a selective 5-ht2c receptor agonist that has been fda approved as a treatment for obesity. the most frequently reported side-effects of lor include nausea and headache, which can be dose limiting. we have previously reported that in the rat, while lor produced unconditioned signs characteristic of nausea/malaise, the highly selective 5-ht2c agonist cp-809101 (cp) produced fewer equivalent signs. because this may indicate a subclass of 5-ht2c agonists having better tolerabilit ... | 2017 | 28338324 |
| stable high-level expression of factor viii in chinese hamster ovary cells in improved elongation factor-1 alpha-based system. | recombinant factor viii (fviii), used for haemophilia a therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. deletion variant of fviii, in which the entire domain b is replaced by a short linker peptide, was approved for medical use. efficacy and safety of this fviii deletion variant are similar to full-length fviii preparations while the level of production in cho cells is substantially higher. typical levels of productivity for cho ... | 2017 | 28340620 |
| a high density cho-s transient transfection system: comparison of expicho and expi293. | chinese hamster ovary (cho) cells are the principal mammalian host used for stable cell line generation and biotherapeutic protein production. until recently, production of milligrams to grams of protein in cho transient systems was challenging. as such, human embryonic kidney (hek293) cells are the most common mammalian cell type used for transient transfection. the post-translational modifications (ptms) of a protein are dictated in part by the cell line used for expression, and changes in ptm ... | 2017 | 28342833 |
| antioxidant and cytoprotective effects of morin against hydrogen peroxide-induced oxidative stress are associated with the induction of nrf-2‑mediated ho-1 expression in v79-4 chinese hamster lung fibroblasts. | natural phytochemicals of plant origin, including flavonoids, have been found to be potent antioxidants providing beneficial effects against oxidative stress-related diseases. the present study was carried out to investigate the antioxidant properties of morin, a flavonoid originally isolated from the flowering plants of the moraceae family. superoxide dismutase (sod)‑like activity and 2,2'‑azino‑bis‑(3‑ethylbenzothiazoline‑6‑sulfonic acid) (abts•+) radical scavenging activity were determined. w ... | 2017 | 28350063 |
| development of a cho-based cell-free platform for synthesis of active monoclonal antibodies. | chinese hamster ovary (cho) cells are routinely optimized to stably express monoclonal antibodies (mabs) at high titers. at the early stages of lead isolation and optimization, hundreds of sequences for the target protein of interest are screened. typically, cell-based transient expression technology platforms are used for expression screening, but these can be time- and resource-intensive. here, we have developed a cell-free protein synthesis (cfps) platform utilizing a commercially available c ... | 2017 | 28350472 |
| production of recombinant human acid α-glucosidase with high-mannose glycans in gnt1 rice for the treatment of pompe disease. | lysosomal storage diseases are a group of inherited metabolic disorders. patients are treated with enzyme replacement therapy (ert), in which the replacement enzymes are required to carry terminal mannose or mannose 6-phosphate residues to allow efficient uptake into target cells and tissues. n-acetylglucosaminyltransferase-i (gnti) mediates n-glycosylation in the cis cisternae of the golgi apparatus by adding n-acetylglucosamine to the exposed terminal mannose residue of core n-glycan structure ... | 2017 | 28363873 |
| label-free protein quantification of sodium butyrate treated cho cells by esi-uhr-tof-ms. | effects of butyrate on cho producer cells are contradictory, promoting productivity and at the same time repressing proliferation. though in previous omics studies the background of butyrate impact on producer cells has been investigated, the knowledge about the mechanism is still very limited. as previous proteomic results on this field are mainly based on 2de-gels, we conducted a label-free ms quantification, based on fast high resolution esi-ms and a straight forward software solution, to gai ... | 2017 | 28363874 |
| the hybrid search: a mass spectral library search method for discovery of modifications in proteomics. | we present a mass spectral library-based method to identify tandem mass spectra of peptides that contain unanticipated modifications and amino acid variants. we describe this as a "hybrid" method because it combines matching both ion m/z and mass losses. the mass loss is the difference between the mass of an ion peak and the mass of its precursor. this difference, termed deltamass, is used to shift the product ions in the library spectrum that contain the modification, thereby allowing library p ... | 2017 | 28367633 |
| segmentation and quantitative analysis of apoptosis of chinese hamster ovary cells from fluorescence microscopy images. | accurate and fast quantitative analysis of living cells from fluorescence microscopy images is useful for evaluating experimental outcomes and cell culture protocols. an algorithm is developed in this work to automatically segment and distinguish apoptotic cells from normal cells. the algorithm involves three steps consisting of two segmentation steps and a classification step. the segmentation steps are: (i) a coarse segmentation, combining a range filter with a marching square method, is used ... | 2017 | 28367787 |
| coexpressed class b g protein-coupled secretin and glp-1 receptors self- and cross-associate: impact on pancreatic islets. | class b guanine nucleotide-binding protein (g protein)-coupled receptors form symmetrical homodimeric complexes along the lipid face of transmembrane segment 4 (tm4) and can form heterodimeric complexes, although their structure is unknown. the current study demonstrates that the lipid face of tm4 is also the predominant determinant for formation of heteroreceptor complexes between two class b receptors, secretin receptor (secr) and glucagonlike peptide-1 receptor (glp-1r), which are expressed o ... | 2017 | 28368447 |
| n-terminal or signal peptide sequence engineering prevents truncation of human monoclonal antibody light chains. | monoclonal antibodies (mabs) contain short n-terminal signal peptides on each individual polypeptide that comprises the mature antibody, targeting them for export from the cell in which they are produced. the signal peptide is cleaved from each heavy chain (hc) and light chain (lc) polypeptide after translocation to the er and prior to secretion. this process is generally highly efficient, producing a high proportion of correctly cleaved hc and lc polypeptides. however, mis-cleavage of the signa ... | 2017 | 28369727 |
| investigation of toxicity and mutagenicity of cold atmospheric argon plasma. | cold atmospheric argon plasma is recognized as a new contact free approach for the decrease of bacterial load on chronic wounds in patients. so far very limited data are available on its toxicity and mutagenicity on eukaryotic cells. thus, the toxic/mutagenic potential of cold atmospheric argon plasma using the microplaster β(®) , which has been used efficiently in humans treating chronic and acute wounds, was investigated using the xtt assay in keratinocytes and fibroblasts and the hgprt (hypox ... | 2017 | 28370324 |
| low glucose concentrations within typical industrial operating conditions have minimal effect on the transcriptome of recombinant cho cells. | typically, mammalian cell culture medium contains high glucose concentrations that are analogous to diabetic levels in humans, suggesting that mammalian cells are cultivated in excessive glucose. using rna-seq, this study characterized the chinese hamster ovary (cho) cell transcriptome under two glucose concentrations to assess the genetic effects associated with metabolic pathways, in addition to other global responses. the initial extracellular glucose concentrations used represented high (30 ... | 2017 | 28371311 |
| principles and approach to developing mammalian cell culture media for high cell density perfusion process leveraging established fed-batch media. | perfusion medium was successfully developed based on our fed-batch platform basal and feed media. a systematic development approach was undertaken by first optimizing the ratios of fed-batch basal and feed media followed by targeted removal of unnecessary and redundant components. with this reduction in components, the medium could then be further concentrated by 2× to increase medium depth. the medium osmolality was also optimized where we found ∼360 mosm/kg was desirable resulting in a residua ... | 2017 | 28371394 |
| a strategy to accelerate protein production from a pool of clones in chinese hamster ovary cells for toxicology studies. | in the biopharmaceutical industry, a clonally derived cell line is typically used to generate material for investigational new drug (ind)-enabling toxicology studies. the same cell line is then used to generate material for clinical studies. if a pool of clones can be used to produce material for ind-enabling toxicology studies ("pool for tox strategy") during the time a lead clone is being selected for clinical material production, the toxicology studies can be accelerated significantly (approx ... | 2017 | 28371489 |
| mir-143 targets mapk7 in cho cells and induces a hyperproductive phenotype to enhance production of difficult-to-express proteins. | in recent years, the number of complex but clinically effective biologicals such as multi-specific antibody formats and fusion proteins has increased dramatically. however, compared to classical monoclonal antibodies (mabs), these rather artificially designed therapeutic proteins have never undergone millions of years of evolution and thus often turn out to be difficult-to-express using mammalian expression systems such as chinese hamster ovary (cho) cells. in order to provide access to these so ... | 2017 | 28371547 |
| dna double-strand breaks induced byfractionated neutron beam irradiation for boron neutron capture therapy. | to use the 53bp1 foci assay to detect dna double-strand breaks induced by fractionated neutron beam irradiation of normal cells. | 2017 | 28373429 |
| arabinosylation of recombinant human immunoglobulin-based protein therapeutics. | protein glycosylation is arguably the paramount post-translational modification on recombinant glycoproteins, and highly cited in the literature for affecting the physiochemical properties and the efficacy of recombinant glycoprotein therapeutics. glycosylation of human immunoglobulins follows a reasonably well-understood metabolic pathway, which gives rise to a diverse range of asparagine-linked (n-linked), or serine/threonine-linked (o-linked) glycans. in n-linked glycans, fucose levels have b ... | 2017 | 28375048 |
| the short neuropeptide f modulates olfactory sensitivity of bactrocera dorsalis upon starvation. | the insect short neuropeptide f (snpf) family has been shown to modulate diverse physiological processes, such as feeding, appetitive olfactory behavior, locomotion, sleep homeostasis and hormone release. in this study, we identified the snpf (bdsnpf) and its receptor (bdsnpfr) in an important agricultural pest, the oriental fruit fly bactrocera dorsalis (hendel). afterwards, the receptor cdna was functionally expressed in chinese hamster ovary cell lines. activation of bdsnpfr by snpf peptides ... | 2017 | 28377097 |
| optimizing conditions for calcium phosphate mediated transient transfection. | calcium phosphate mediated transfection has been used for delivering dna into mammalian cells in excess of 30 years due to its most low cost for introducing recombinant dna into culture cells. however, multiple factors affecting the transfect efficiency are commonly recognized meanwhile for years, the low transfection efficiency of this approach on higher differentiated and non-tumor cells such as cho and c2c12 limits its application on research. | 2017 | 28386188 |
| enhancing the toolbox to study il-17a in cattle and sheep. | the development of methods to detect cytokine expression by t cell subsets in ruminants is fundamental to strategic development of new livestock vaccines for prevention of infectious diseases. it has been possible to detect t cell expression of ifn-γ, il-4 and il-10 in ruminants for many years but methods to detect expression of il-17a are relatively limited. to address this gap in capability we have cloned bovine and ovine il-17a cdnas and expressed biologically-active recombinant proteins in c ... | 2017 | 28388924 |
| peroxisome biogenesis: a novel inducible pex19 splicing variant is involved in early stages of peroxisome proliferation. | pex19p harbouring a prenylation caax box functions as a chaperone and transporter for peroxisomal membrane proteins in membrane assembly. by functional phenotype-complementation assay using a pex19 chinese hamster ovary cell mutant zp119, we herein cloned a rat cdna encoding a protein similar to pex19p, but with a c-terminal hydrophobic segment in place of the caax box region. the transcript of this gene was highly induced by treatment of rats with a peroxisome proliferator, clofibrate, hence te ... | 2017 | 28391327 |
| phic31 integrase can improve the efficiency of different construct designs for monoclonal antibody expression in cho cells. | several types of expression vectors have been used for recombinant protein expression in chinese hamster ovary cells (cho) which usually result in variable and unstable levels of expression. | 2017 | 28400295 |
| impact of anatase titanium dioxide nanoparticles on mutagenic and genotoxic response in chinese hamster lung fibroblast cells (v-79): the role of cellular uptake. | the unique physico-chemical properties of nano crystalline anatase titanium dioxide nanoparticles (tio2 nps) render them with different biological and chemical activities. hence, it is widely used in industrial and consumer applications. previous studies have shown the genotoxicity of tio2 nps. however, there is a paucity of data regarding mutagenicity of these nps. in the present study, the cellular uptake, sub-cellular localization, cytotoxicity and short term dna interaction of tio2 nps (1-10 ... | 2017 | 28400324 |
| novel selective β1-adrenoceptor antagonists for concomitant cardiovascular and respiratory disease. | β-blockers reduce mortality and improve symptoms in people with heart disease; however, current clinically available β-blockers have poor selectivity for the cardiac β1-adrenoceptor (ar) over the lung β2-ar. unwanted β2-blockade risks causing life-threatening bronchospasm and reduced efficacy of β2-agonist emergency rescue therapy. thus, current life-prolonging β-blockers are contraindicated in patients with both heart disease and asthma. here, we describe ndd-713 and -825, novel highly β1-selec ... | 2017 | 28400472 |
| transient dominant host-range selection using chinese hamster ovary cells to generate marker-free recombinant viral vectors from vaccinia virus. | recombinant vaccinia viruses (rvacvs) are promising antigen-delivery systems for vaccine development that are also useful as research tools. two common methods for selection during construction of rvacv clones are (i) co-insertion of drug resistance or reporter protein genes, which requires the use of additional selection drugs or detection methods, and (ii) dominant host-range selection. the latter uses vacv variants rendered replication-incompetent in host cell lines by the deletion of host-ra ... | 2017 | 28403810 |
| genome-wide screening uncovers the significance of n-sulfation of heparan sulfate as a host cell factor for chikungunya virus infection. | the molecular mechanisms underlying chikungunya virus (chikv) infection are poorly characterized. in this study, we analyzed the host factors involved in chikv infection using genome-wide screening. human haploid hap1 cells, into which an exon-trapping vector was introduced, were challenged with a vesicular stomatitis virus pseudotype bearing the chikv e3 to e1 envelope proteins. analysis of genes enriched in the cells resistant to the pseudotyped virus infection unveiled a critical role of n-su ... | 2017 | 28404855 |
| genotoxicity testing of sodium formononetin-3'-sulphonate (sul-f) by assessing bacterial reverse mutation, chromosomal aberrations and micronucleus tests. | as part of a safety evaluation, we evaluated the potential genotoxicity of sodium formononetin-3'-sulphonate (sul-f) using bacterial reverse mutation assay, chromosomal aberrations detection, and mouse micronucleus test. in bacterial reverse mutation assay using five strains of salmonella typhimurium (ta97, ta98, ta100, ta102 and ta1535), sul-f (250, 500, 1000, 2000, 4000 μg/plate) did not increase the number of revertant colonies in any tester strain with or without s9 mix. in a chromosomal ass ... | 2017 | 28408196 |
| smurf1 inhibits integrin activation by controlling kindlin-2 ubiquitination and degradation. | integrin activation is an indispensable step for various integrin-mediated biological functions. kindlin-2 is known to coactivate integrins with talin; however, molecules that restrict integrin activation are elusive. here, we demonstrate that the e3 ubiquitin ligase smurf1 controls the amount of kindlin-2 protein in cells and hinders integrin activation. smurf1 interacts with and promotes kindlin-2 ubiquitination and degradation. smurf1 selectively mediates degradation of kindlin-2 but not tali ... | 2017 | 28408404 |
| azithromycin causes a novel proarrhythmic syndrome. | the widely used macrolide antibiotic azithromycin increases risk of cardiovascular and sudden cardiac death, although the underlying mechanisms are unclear. case reports, including the one we document here, demonstrate that azithromycin can cause rapid, polymorphic ventricular tachycardia in the absence of qt prolongation, indicating a novel proarrhythmic syndrome. we investigated the electrophysiological effects of azithromycin in vivo and in vitro using mice, cardiomyocytes, and human ion chan ... | 2017 | 28408648 |
| generation of peroxisome-deficient somatic animal cell mutants. | cell mutants with a genetic defect affecting various cellular phenotypes are widely utilized as a powerful tool in genetic, biochemical, and cell biological research. more than a dozen complementation groups of animal somatic mutant cells defective in peroxisome biogenesis have been successfully isolated in chinese hamster ovary (cho) cells and used as a model system reflecting fatal human severe genetic disorders named peroxisome biogenesis disorders (pbd). isolation and characterization of per ... | 2017 | 28409474 |
| identification and control of novel growth inhibitors in fed-batch cultures of chinese hamster ovary cells. | chinese hamster ovary (cho) cells in culture are known to consume large amounts of nutrients and divert most of them toward byproducts, some of which, including lactate and ammonia, are known to be toxic in nature. glucose limitation strategies can successfully control lactate accumulation in fed-batch cultures yielding higher peak cell densities and titers. interestingly, even in such optimized cultures, cell growth slows and eventually stops, indicating the emergence of other factors that nega ... | 2017 | 28409820 |
| a comparative genomic hybridization approach to study gene copy number variations among chinese hamster cell lines. | chinese hamster ovary (cho) cells are aneuploid in nature. the genome of recombinant protein producing cho cell lines continuously undergoes changes in its structure and organization. we analyzed nine cell lines, including parental cell lines, using a comparative genomic hybridization (cgh) array focused on gene-containing regions. the comparison of cgh with copy-number estimates from sequencing data showed good correlation. hierarchical clustering of the gene copy number variation data from cgh ... | 2017 | 28409824 |
| modulation and modeling of monoclonal antibody n-linked glycosylation in mammalian cell perfusion reactors. | mammalian cell perfusion cultures are gaining renewed interest as an alternative to traditional fed-batch processes for the production of therapeutic proteins, such as monoclonal antibodies (mab). the steady state operation at high viable cell density allows the continuous delivery of antibody product with increased space-time yield and reduced in-process variability of critical product quality attributes (cqa). in particular, the production of a confined mab n-linked glycosylation pattern has t ... | 2017 | 28409838 |
| ryanodine and ip3 receptor-mediated calcium signaling play a pivotal role in neurological infrared laser modulation. | pulsed infrared (ir) laser energy has been shown to modulate neurological activity through both stimulation and inhibition of action potentials. while the mechanism(s) behind this phenomenon is (are) not completely understood, certain hypotheses suggest that the rise in temperature from ir exposure could activate temperature- or pressure-sensitive ion channels or create pores in the cellular outer membrane, allowing an influx of typically plasma-membrane-impermeant ions. studies using fluorescen ... | 2017 | 28413806 |
| novel high-throughput cell-based hybridoma screening methodology using the celigo image cytometer. | hybridoma screening is a critical step for antibody discovery, which necessitates prompt identification of potential clones from hundreds to thousands of hybridoma cultures against the desired immunogen. technical issues associated with elisa- and flow cytometry-based screening limit accuracy and diminish high-throughput capability, increasing time and cost. conventional elisa screening with coated antigen is also impractical for difficult-to-express hydrophobic membrane antigens or multi-chain ... | 2017 | 28414024 |
| enhancing protein production yield from chinese hamster ovary cells by crispr interference. | chinese hamster ovary (cho) cells are an important host for biopharmaceutical production. generation of stable cho cells typically requires cointegration of dhfr and a foreign gene into chromosomes and subsequent methotrexate (mtx) selection for coamplification of dhfr and foreign gene. crispr interference (crispri) is an emerging system that effectively suppresses gene transcription through the coordination of dcas9 protein and guide rna (grna). however, crispri has yet to be exploited in cho c ... | 2017 | 28418635 |
| identification of a host cell protein impurity in therapeutic protein, p1. | residual host cell proteins (hcps) are process-related impurities present in biotherapeutics that can pose safety health risks to patients. an adequate control of hcp levels in the final product, and demonstration of hcp clearance throughout a product manufacturing process is critical for all biotherapeutic products. developing effective downstream purification processes can be challenging as hcps and product proteins may possess an affinity for each other or have similar physicochemical propert ... | 2017 | 28419935 |
| characterization of the conbercept gene localization in dhfr- amplified cho cells using fluorescence in situ hybridization. | chinese-hamster ovary (cho) cells are most widely used for mammalian protein expression. after integration into the cho genome, the exogenous gene may be lost in the process of large-scale protein production due to the removal of related selection pressures. therefore, it is necessary to test its stability in the genome. conbercept is a fusion protein that specifically binds to the various isoforms of vascular endothelial growth factor (vegf)-a, vegf-b, and placental growth factor (plgf), thereb ... | 2017 | 28420611 |
| acute exposure to dehp metabolite, mehp cause genotoxicity, mutagenesis and carcinogenicity in mammalian chinese hamster ovary cells. | di-(2-ethylhexyl) phthalate (dehp), the common plasticizer used in the production of polyvinyl chloride, can be converted to the more potent metabolite mono-ethylhexyl phthalate (mehp). epidemiological studies have shown an association with elevated induction of rat hepatic cancer and reproductive toxicity in response to mehp exposure. however, the mechanism of genotoxicity and carcinogenicity induced by mehp treatment remains unclear. as a means to elucidate the mechanisms of action, lethality ... | 2017 | 28426879 |
| the pseudomonas aeruginosa lectin leca triggers host cell signalling by glycosphingolipid-dependent phosphorylation of the adaptor protein crkii. | the human pathogen pseudomonas aeruginosa induces phosphorylation of the adaptor protein crkii by activating the non-receptor tyrosine kinase abl to promote its uptake into host cells. so far, specific factors of p. aeruginosa, which induce abl/crkii signalling, are entirely unknown. in this research, we employed human lung epithelial cells h1299, chinese hamster ovary cells and p. aeruginosa wild type strain pao1 to study the invasion process of p. aeruginosa into host cells by using microbiolo ... | 2017 | 28428058 |
| enhanced production of recombinant proteins by a small molecule protein synthesis enhancer in combination with an antioxidant in recombinant chinese hamster ovary cells. | the improvement in the production of recombinant proteins has been linked in a number of small molecules such as carboxylic acids to the inhibition of histone deacetylase, leading to increased transcription of genes. however, carboxylic acids such as pentanoic acid and butanoic acid have been shown to promote an apoptotic response in chinese hamster ovary (cho) cell culture. supplementation of cultures with antioxidants has shown the ability to reduce the apoptotic response of carboxylic acid su ... | 2017 | 28432429 |
| identification of indothiazinone as a natural antiplatelet agent. | cardiovascular disease, which is caused by unregulated platelet aggregation, is one of the main causes of deaths worldwide. many studies have focused on natural products with antiplatelet effects as a safe alternative therapy to prevent the disease. in this context, an in-house chemical library was screened to find natural products capable of inhibiting the interaction between platelet integrin αiibβ3 and fibrinogen, which is an essential step in platelet aggregation. on the basis of the screeni ... | 2017 | 28432753 |
| investigation of triamterene as an inhibitor of the tgr5 receptor: identification in cells and animals. | g-protein-coupled bile acid receptor 1 (gpbar1, also known as tgr5) has been shown to participate in glucose homeostasis. in animal models, a tgr5 agonist increases incretin secretion to reduce hyperglycemia. many agonists have been developed for clinical use. however, the effects of tgr5 blockade have not been studied extensively, with the exception of studies using tgr5 knockout mice. therefore, we investigated the potential effect of triamterene on tgr5. | 2017 | 28435224 |
| comparison of the behavior of cho cells during cultivation in 24-square deep well microplates and conventional shake flask systems. | in biopharmaceutical production, the optimization of cell culture media and supplementation is a vital element of process development. optimization is usually achieved through the screening of multiple media, feed and feeding strategies. however, most screening is performed in shake flasks, which makes the screening process very time consuming and inefficient. the use of small scale culture systems for the screening process can aid in the ability to screen multiple formulations during process de ... | 2014 | 28435799 |
| overexpression of the regulatory subunit of glutamate-cysteine ligase enhances monoclonal antibody production in cho cells. | for decades, chinese hamster ovary (cho) cells have been the preferred host for therapeutic monoclonal antibody (mab) production; however, increasing mab titer by rational engineering remains a challenge. our previous proteomic analysis in cho cells suggested that a higher content of glutathione (gsh) might be related to higher productivity. gsh is an important antioxidant, cell detoxifier, and is required to ensure the formation of native disulfide bonds in proteins. to investigate the involvem ... | 2017 | 28436007 |
| prevalence and clinical relevance of thyroid stimulating hormone receptor-blocking antibodies in autoimmune thyroid disease. | the prevalence and clinical relevance of thyroid stimulating hormone (tsh) receptor (tshr) blocking antibodies (tbab) in patients with autoimmune thyroid disease (aitd) was investigated. serum tbab were measured with a reporter gene bioassay using chinese hamster ovary cells. blocking activity was defined as percentage inhibition of luciferase expression relative to induction with bovine tsh alone (cut-off 40% inhibition). all samples were measured for tshr stimulatory antibody (tsab) and tshr b ... | 2017 | 28439882 |
| investigation of the bystander effect in cho-k1 cells. | investigation of the bystander effect in chinese hamster ovary cells (cho-k1) co-cultured with cells irradiated in the dose range of 0.1-4 gy of high let 12c ions and x-rays. | 2014 | 28443197 |
| characterization of relative biological effectiveness for conventional radiation therapy: a comparison of clinical 6 mv x-rays and 137cs. | various types of radiation are utilized in the treatment of cancer. equal physical doses of different radiation types do not always result in the same amount of biological damage. in order to account for these differences, a scaling factor known as the relative biological effectiveness (rbe) can be used. 137cesium (137cs) has been used as a source of radiation in a significant body of radiation therapy research. however, high-energy x-rays, such as 6 mv x-rays, are currently used clinically to t ... | 2017 | 28444207 |
| induction of micronuclei and cell cycle arrest by some tri- and tetrachlorobiphenyls in mammalian cells deficient in xenobiotic-metabolizing enzymes. | polychlorinated biphenyls (pcbs) are persistent organic pollutants with continued public health concerns. the lower chlorinated biphenyls are supposed to be mutagenic following metabolic activation. however, in a preliminary study, we recently observed induction of micronuclei by several pcbs in a subclone of chinese hamster v79 cell line, v79-mz, which is deficient in xenobiotic-metabolizing enzyme activities. in this study, metabolism-free genotoxicity of pcbs was investigated, using 10 tri- a ... | 2017 | 28444994 |
| characterization of four latin american families confirms previous findings and reveals novel features of acid-labile subunit deficiency. | acid-labile subunit deficiency (aclsd), caused by inactivating mutations in both igfals gene alleles, is characterized by marked reduction in igf-i and igfbp-3 levels associated with mild growth retardation. the aim of this study was to expand the known phenotype and genetic characteristics of aclsd by reporting data from four index cases and their families. | 2017 | 28445628 |
| antigenotoxic properties of paliurus spina-christi mill fruits and their active compounds. | paliurus spina-christi mill. (ps) fruits are widely used for different medical purposes in turkey. like in many medicinal herbs the studies concerning their activity, the activities of ps are also not well clarified. the aim of this study is to evaluate the antigenotoxicity of the compounds isolated and identified from the extracts of ps fruits. | 0 | 28446228 |