Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| meta-analysis evidence of maternal lineages in chinese tibetan sheep using mtdna d-loop panel. | the tibetan sheep is an indigenous breed living in the entire tibetan plateau, and its origin and phylogenic relationships are still uncertain and controversial. in this study, we analyzed partial mtdna d-loop sequences of 156 chinese tibetan sheep individuals from 12 distributed geographic ecotype populations. phylogenetic analysis indicated that three maternal lineages (haplogroups a, b and c) were found in this breed and that ovis vignei and ovis ammon have possibly contributed to the origina ... | 2017 | 27159711 |
| the sheep (ovis aries) muscle proteome: decoding the mechanisms of tolerance to seasonal weight loss using label-free proteomics. | seasonal weight loss (swl) is one of the most pressing issues in animal production in the tropics and mediterranean. this work aims to characterize muscle proteome changes as a consequence of swl in meat producing sheep, using a label-free proteomics approach. we compare three breeds: the australian merino (swl susceptible), the damara (swl tolerant) and the dorper (swl intermediate tolerance). we identified 668 proteins of the sheep proteome, 95 with differential regulation. also we observe tha ... | 2017 | 28366878 |
| provision of physiological data and reference values in awake and anaesthetized female sheep aged 6-12 months. | to provide physiological data and reference values in awake and anaesthetized sheep aged 6-12 months. | 2017 | 28330726 |
| endogenous retroviral insertions indicate a secondary introduction of domestic sheep lineages to the caucasus and central asia between the bronze and iron age. | sheep were one of the first livestock species domesticated by humans. after initial domestication in the middle east they were spread across eurasia. the modern distribution of endogenous jaagsiekte sheep retrovirus insertions in domestic sheep breeds suggests that over the course of millennia, successive introductions of improved lineages and selection for wool quality occurred in the mediterranean region and most of asia. here we present a novel ancient dna approach using data of endogenous re ... | 2017 | 28632161 |
| differentiation potential of neural stem cells derived from fetal sheep. | neural stem cells (nscs) are multipotent stem cells that can differentiate into many cell types in vitro. in this study, we isolated and established an nsc line from fetal ovis aries. based on the results of immunofluorescence staining, nscs expressed nestin, pax6 and map2. moreover, a reverse transcription-polymerase chain reaction assay was used to biologically characterize the cell line. nscs were induced to differentiate into neurogenic cells in vitro. they expressed map2, glial fibrillary a ... | 2017 | 30460074 |
| isolation and identification of mycoplasma agalactiae by culture and polymerase chain reaction methods in the sheep herds in guilan province, iran. | contagious agalactia is an infectious syndrome of sheep that is characterized by mastitis with reduction of milk production, arthritis, abortion, and keratoconjunctivitis. the disease is rapidly spread by the contact of the infected animals with the healthy ones. domestic sheep and goats of both sexes can be infected at an equivalent frequency. most of the researchers use culture and molecular methods for the isolation and identification of mycoplasma. mycoplasma agalactiae is the main cause of ... | 2017 | 30315697 |
| new polymorphism in the 5' flanking region of igf-1 gene and its association with wool traits in egyptian barki sheep. | insulin-like growth factor-1 gene (igf-1) is considered as a major candidate gene for the economic traits of animal production. polymorphism of 5' flanking region of igf-1 gene in barki sheep (n = 91) and its association with wool traits were studied using the polymerase chain reaction coupled with single-strand conformation polymorphism technique (pcr-sscp), pcr-restriction fragment length polymorphism (pcr-rflp), sequence analysis and different measurements of wool traits (clean fleece weight ... | 2017 | 30647684 |
| inbreeding depression by environment interactions in a free-living mammal population. | experimental studies often find that inbreeding depression is more severe in harsh environments, but the few studies of in situ wild populations available to date rarely find strong support for this effect. we investigated evidence for inbreeding depression by environment interactions in nine traits in the individually monitored soay sheep population of st kilda, using genomic inbreeding coefficients based on 37 037 single-nucleotide polymorphism loci, and population density as an axis of enviro ... | 2016 | 27876804 |
| conserved genetic architecture underlying individual recombination rate variation in a wild population of soay sheep (ovis aries). | meiotic recombination breaks down linkage disequilibrium (ld) and forms new haplotypes, meaning that it is an important driver of diversity in eukaryotic genomes. understanding the causes of variation in recombination rate is important in interpreting and predicting evolutionary phenomena and in understanding the potential of a population to respond to selection. however, despite attention in model systems, there remains little data on how recombination rate varies at the individual level in nat ... | 2016 | 27029733 |
| interspecific interactions and the scope for parent-offspring conflict: high mite density temporarily changes the trade-off between offspring size and number in the burying beetle, nicrophorus vespilloides. | parents have a limited amount of resources to invest in reproduction and commonly trade-off how much they invest in offspring size (or quality) versus brood size. a negative relationship between offspring size and number has been shown in numerous taxa and it underpins evolutionary conflicts of interest between parents and their young. for example, previous work on vertebrates shows that selection favours mothers that produce more offspring, at the expense of individual offspring size, yet favou ... | 2016 | 26985819 |
| a further cost for the sicker sex? evidence for male-biased parasite-induced vulnerability to predation. | males are typically the sicker sex. data from multiple taxa indicate that they are more likely to be infected with parasites, and are less "tolerant," or less able to mitigate the fitness costs of a given infection, than females. one cost of infection for many animals is an increased probability of being captured by a predator. a clear, hitherto untested, prediction is therefore that this parasite-induced vulnerability to predation is more pronounced among males than females. we tested this pred ... | 2016 | 27066240 |
| characterization and comparative analyses of transcriptomes for in vivo and in vitro produced peri-implantation conceptuses and endometria from sheep. | an increasing number of reports indicate that in vitro fertilization (ivf) is highly associated with long‑term side effects on embryonic and postnatal development, and can sometimes result in embryonic implant failure. while high‑throughput gene expression analysis has been used to explore the mechanisms underlying ivf-induced side effects on embryonic development, little is known about the effects of ivf on conceptus-endometrial interactions during the peri-implantation period. using sheep as a ... | 2016 | 26946921 |
| a genome-wide association study identifies a genomic region for the polycerate phenotype in sheep (ovis aries). | horns are a cranial appendage found exclusively in bovidae, and play important roles in accessing resources and mates. in sheep (ovies aries), horns vary from polled to six-horned, and human have been selecting polled animals in farming and breeding. here, we conducted a genome-wide association study on 24 two-horned versus 22 four-horned phenotypes in a native chinese breed of sishui fur sheep. together with linkage disequilibrium (ld) analyses and haplotype-based association tests, we identifi ... | 2016 | 26883901 |
| impact of parity on ewe vaginal mechanical properties relative to the nonhuman primate and rodent. | parity is the leading risk factor for the development of pelvic organ prolapse. to assess the impact of pregnancy and delivery on vaginal tissue, researchers commonly use nonhuman primate (nhp) and rodent models. the purpose of this study was to evaluate the ewe as an alternative model by investigating the impact of parity on the ewe vaginal mechanical properties and collagen structure. | 2016 | 26872644 |
| cardiotoxicity of senna occidentalis in sheep (ovis aries). | the cardiotoxicity of coffee senna (senna occidentalis) was investigated in sheep that were fed diets containing its seeds, which are recognized as the most poisonous part of such weed. dianthrone, the main toxic component of s. occidentalis, is known to impair mitochondrial oxidative phosphorylation, leading to myofiber degeneration. in this study, fifteen ewes were fed 0%, 2% or 4% of seeds of s. occidentalis for 63 days. non-specific markers of myocyte injury and electrocardiograms were under ... | 2016 | 26894038 |
| the effect of weight loss on the muscle proteome in the damara, dorper and australian merino ovine breeds. | seasonal weight loss (swl) is an important constraint, limiting animal production in the tropics and the mediterranean. as a result, the study of physiological and biochemical mechanisms by which domestic animal breeds respond to swl is important to those interested in animal breeding and the improvement thereof. to that end, the study of the proteome has been instrumental in gathering important information on physiological mechanisms, including those underlying swl. in spite of that, little inf ... | 2016 | 26828937 |
| intense selective hunting leads to artificial evolution in horn size. | the potential for selective harvests to induce rapid evolutionary change is an important question for conservation and evolutionary biology, with numerous biological, social and economic implications. we analyze 39 years of phenotypic data on horn size in bighorn sheep (ovis canadensis) subject to intense trophy hunting for 23 years, after which harvests nearly ceased. our analyses revealed a significant decline in genetic value for horn length of rams, consistent with an evolutionary response t ... | 2016 | 27099619 |
| conservation genetics in chinese sheep: diversity of fourteen indigenous sheep (ovis aries) using microsatellite markers. | the domestic sheep (ovis aries) has been an economically and culturally important farm animal species since its domestication around the world. a wide array of sheep breeds with abundant phenotypic diversity exists including domestication and selection as well as the indigenous breeds may harbor specific features as a result of adaptation to their environment. the objective of this study was to investigate the population structure of indigenous sheep in a large geographic location of the chinese ... | 2016 | 26865968 |
| ovine rumen papillae biopsy via oral endoscopy; a rapid and repeatable method for serial sampling. | to explore and validate the utility of rumen endoscopy for collection of rumen papillae for gene expression measurement. | 2016 | 26642120 |
| vitamin d status predicts reproductive fitness in a wild sheep population. | vitamin d deficiency has been associated with the development of many human diseases, and with poor reproductive performance in laboratory rodents. we currently have no idea how natural selection directly acts on variation in vitamin d metabolism due to a total lack of studies in wild animals. here, we measured serum 25 hydroxyvitamin d (25(oh)d) concentrations in female soay sheep that were part of a long-term field study on st kilda. we found that total 25(oh)d was strongly influenced by age, ... | 2016 | 26757805 |
| missense mutation in disc1 c-terminal coiled-coil has gsk3β signaling and sex-dependent behavioral effects in mice. | disrupted-in-schizophrenia 1 (disc1) is a risk factor for schizophrenia and affective disorders. the full-length disc1 protein consists of an n-terminal 'head' domain and a c-terminal tail domain that contains several predicted coiled-coils, structural motifs involved in protein-protein interactions. to probe the in vivo effects of missense mutation of disc1's c-terminal tail, we tested mice carrying mutation d453g within a predicted α-helical coiled-coil region. we report that, relative to wild ... | 2016 | 26728762 |
| detection and replication of qtl underlying resistance to gastrointestinal nematodes in adult sheep using the ovine 50k snp array. | persistence of gastrointestinal nematode (gin) infection and the related control methods have major impacts on the sheep industry worldwide. based on the information generated with the illumina ovinesnp50 beadchip (50 k chip), this study aims at confirming quantitative trait loci (qtl) that were previously identified by microsatellite-based genome scans and identifying new qtl and allelic variants that are associated with indicator traits of parasite resistance in adult sheep. we used a commerci ... | 2016 | 26791855 |
| the complex relationship between metals and carbonic anhydrase: new insights and perspectives. | carbonic anhydrase is a ubiquitous metalloenzyme, which catalyzes the reversible hydration of co₂ to hco₃(-) and h⁺. metals play a key role in the bioactivity of this metalloenzyme, although their relationships with ca have not been completely clarified to date. the aim of this review is to explore the complexity and multi-aspect nature of these relationships, since metals can be cofactors of ca, but also inhibitors of ca activity and modulators of ca expression. moreover, this work analyzes new ... | 2016 | 26797606 |
| anthelmintic treatment does not change foraging strategies of female eastern grey kangaroos, macropus giganteus. | large mammalian herbivores are commonly infected with gastrointestinal helminths. heavily parasitised hosts are likely to have increased nutritional requirements and would be predicted to increase their food intake to compensate for costs of being parasitised, but experimental tests of the impacts of these parasites on the foraging efficiency of hosts are lacking, particularly in free-ranging wildlife. we conducted a field experiment on a population of free-ranging eastern grey kangaroos (macrop ... | 2016 | 26784582 |
| melatonin: current status and future perspectives in plant science. | melatonin (n-acetyl-5-methoxytryptamine) is a ubiquitous molecule with pleiotropic actions in different organisms. it performs many important functions in human, animals, and plants; these range from regulating circadian rhythms in animals to controlling senescence in plants. in this review, we summarize the available information regarding the presence of melatonin in different plant species, along with highlighting its biosynthesis and mechanisms of action. we also collected the available infor ... | 2016 | 26793210 |
| how fast is fast? eco-evolutionary dynamics and rates of change in populations and phenotypes. | it is increasingly recognized that evolution may occur in ecological time. it is not clear, however, how fast evolution - or phenotypic change more generally - may be in comparison with the associated ecology, or whether systems with fast ecological dynamics generally have relatively fast rates of phenotypic change. we developed a new dataset on standardized rates of change in population size and phenotypic traits for a wide range of species and taxonomic groups. we show that rates of change in ... | 2016 | 26843940 |
| a reappraisal of successive negative contrast in two populations of domestic dogs. | when an anticipated food reward is unexpectedly reduced in quality or quantity, many mammals show a successive negative contrast (snc) effect, i.e. a reduction in instrumental or consummatory responses below the level shown by control animals that have only ever received the lower-value reward. snc effects are believed to reflect an aversive emotional state, caused by the discrepancy between the expected and the actual reward. furthermore, how animals respond to such discrepancy has been suggest ... | 2016 | 26742929 |
| the role of individual traits and environmental factors for diet composition of sheep. | large herbivore consumption of forage is known to affect vegetation composition and thereby ecosystem functions. it is thus important to understand how diet composition arises as a mixture of individual variation in preferences and environmental drivers of availability, but few studies have quantified both. based on 10 years of data on diet composition by aid of microhistological analysis for sheep kept at high and low population density, we analysed how both individual traits (sex, age, body ma ... | 2016 | 26731411 |
| horses discriminate between facial expressions of conspecifics. | in humans, facial expressions are rich sources of social information and have an important role in regulating social interactions. however, the extent to which this is true in non-human animals, and particularly in non-primates, remains largely unknown. therefore we tested whether domestic horses (equus caballus) could discriminate between facial expressions of their conspecifics captured in different contexts, and whether viewing these expressions elicited functionally relevant reactions. horse ... | 2016 | 27995958 |
| applicability of drinking water treatment residue for lake restoration in relation to metal/metalloid risk assessment. | drinking water treatment residue (dwtr), a byproduct generated during potable water production, exhibits a high potential for recycling to control eutrophication. however, this beneficial recycling is hampered by unclear metal/metalloid pollution risks related to dwtr. in this study, the pollution risks of al, as, ba, be, cd, co, cr, cu, fe, mn, mo, ni, pb, and zn due to dwtr application were first evaluated for lake water based on human health risk assessment models and comparison of regulatory ... | 2016 | 27929083 |
| frequency-modulated atomic force microscopy localises viscoelastic remodelling in the ageing sheep aorta. | age-related aortic stiffening is associated with cardiovascular diseases such as heart failure. the mechanical functions of the main structural components of the aorta, such as collagen and elastin, are determined in part by their organisation at the micrometer length scale. with age and disease both components undergo aberrant remodelling, hence, there is a need for accurate characterisation of the biomechanical properties at this length scale. in this study we used a frequency-modulated atomic ... | 2016 | 27479890 |
| cellular and humoral immunity in a wild mammal: variation with age & sex and association with overwinter survival. | immune defenses are expected to be crucial for survival under the considerable parasite pressures experienced by wild animals. however, our understanding of the association between immunity and fitness in nature remains limited due to both the complexity of the vertebrate immune system and the often-limited availability of immune reagents in nonmodel organisms. here, we use methods and reagents developed by veterinary researchers for domestic ungulates on blood samples collected from a wild soay ... | 2016 | 28035261 |
| flurbiprofen-antioxidant mutual prodrugs as safer nonsteroidal anti-inflammatory drugs: synthesis, pharmacological investigation, and computational molecular modeling. | flurbiprofen-antioxidant mutual prodrugs were synthesized to reduce the gastrointestinal (gi) effects associated with flurbiprofen. for reducing the gi toxicity, the free carboxylic group (-cooh) was temporarily masked by esterification with phenolic -oh of natural antioxidants vanillin, thymol, umbelliferone, and sesamol. the in vitro hydrolysis of synthesized prodrugs showed that they were stable in buffer solution at ph 1.2, indicating their stability in the stomach. the synthesized prodrugs ... | 2016 | 27555750 |
| computational drug target screening through protein interaction profiles. | the development of computational methods to discover novel drug-target interactions on a large scale is of great interest. we propose a new method for virtual screening based on protein interaction profile similarity to discover new targets for molecules, including existing drugs. we calculated target interaction profile fingerprints (tipfs) based on chembl database to evaluate drug similarity and generated new putative compound-target candidates from the non-intersecting targets in each pair of ... | 2016 | 27845365 |
| provenancing archaeological wool textiles from medieval northern europe by light stable isotope analysis (δ13c, δ15n, δ2h). | we investigate the origin of archaeological wool textiles preserved by anoxic waterlogging from seven medieval archaeological deposits in north-western europe (c. 700-1600 ad), using geospatial patterning in carbon (δ13c), nitrogen (δ15n) and non-exchangeable hydrogen (δ2h) composition of modern and ancient sheep proteins. δ13c, δ15n and δ2h values from archaeological wool keratin (n = 83) and bone collagen (n = 59) from four sites were interpreted with reference to the composition of modern she ... | 2016 | 27764106 |
| the 1.78-kb insertion in the 3'-untranslated region of rxfp2 does not segregate with horn status in sheep breeds with variable horn status. | the mode of inheritance of horn status in sheep is far more complex than a superficial analysis might suggest. observations, which were mostly based on crossbreeding experiments, indicated that the allele that results in horns is dominant in males and recessive in females, and some authors even speculated about the involvement of more than two alleles. however, all recent genome-wide association analyses point towards a very strong effect of a single autosomal locus on ovine chromosome 10, which ... | 2016 | 27760516 |
| crystal structure of human aldehyde dehydrogenase 1a3 complexed with nad(+) and retinoic acid. | the aldehyde dehydrogenase family 1 member a3 (aldh1a3) catalyzes the oxidation of retinal to the pleiotropic factor retinoic acid using nad(+). the level of aldhs enzymatic activity has been used as a cancer stem cell marker and seems to correlate with tumour aggressiveness. elevated aldh1a3 expression in mesenchymal glioma stem cells highlights the potential of this isozyme as a prognosis marker and drug target. here we report the first crystal structure of human aldh1a3 complexed with nad(+) ... | 2016 | 27759097 |
| implant composed of demineralized bone and mesenchymal stem cells genetically modified with adbmp2/adbmp7 for the regeneration of bone fractures in ovis aries. | adipose-derived mesenchymal stem cells (admscs) are inducible to an osteogenic phenotype by the bone morphogenetic proteins (bmps). this facilitates the generation of implants for bone tissue regeneration. this study evaluated the in vitro osteogenic differentiation of admscs transduced individually and in combination with adenoviral vectors expressing bmp2 and bmp7. moreover, the effectiveness of the implant containing admscs transduced with the adenoviral vectors adbmp2/adbmp7 and embedded in ... | 2016 | 27818692 |
| the impact of group size on welfare indicators of ewes during pregnancy. | group size (gs) and space allowance have major implications for the welfare of production species, however their effects are often confounded. in a previous study we investigated the impact of varying space allowance at constant gs. in the present work we report the consequences of varying gs on pregnant ewes while controlling space allowance. we housed ewes at 6 (gs6) or 12 ewes/enclosure (gs12), while controlling space allowance to 1.5 m2/ewe (3 enclosures/treatment), and necessarily varying e ... | 2016 | 27893817 |
| co-expression analysis and identification of fecundity-related long non-coding rnas in sheep ovaries. | small tail han sheep, including the fecb(b)fecb(b) (han bb) and fecb(+) fecb(+) (han++) genotypes, and dorset sheep exhibit different fecundities. to identify novel long non-coding rnas (lncrnas) associated with sheep fecundity to better understand their molecular mechanisms, a genome-wide analysis of mrnas and lncrnas from han bb, han++ and dorset sheep was performed. after the identification of differentially expressed mrnas and lncrnas, 16 significant modules were explored by using weighted g ... | 2016 | 27982099 |
| comparison of an automatic analysis and a manual analysis of conjunctival microcirculation in a sheep model of haemorrhagic shock. | life-threatening diseases of critically ill patients are known to derange microcirculation. automatic analysis of microcirculation would provide a bedside diagnostic tool for microcirculatory disorders and allow immediate therapeutic decisions based upon microcirculation analysis. | 2016 | 27864774 |
| biological characterization of sheep kidney-derived mesenchymal stem cells. | the aim of the present study was to isolate, culture and characterize sheep metanephric mesenchymal stem cells (mmscs). the mmscs were isolated from the kidney tissue of six-week-old sheep fetus. this study investigated whether primary mmscs could be grown for 26 passages and expressed oct-4, which is involved in the self-renewal of undifferentiated pluripotent stem cells. the mmscs also expressed the renal lineage marker gene pax2, and mesenchymal cell marker genes cd44, fn1 and vim. expression ... | 2016 | 28105130 |
| transcriptome analysis reveals common differential and global gene expression profiles in bluetongue virus serotype 16 (btv-16) infected peripheral blood mononuclear cells (pbmcs) in sheep and goats. | bluetongue is an economically important infectious, arthropod borne viral disease of domestic and wild ruminants, caused by bluetongue virus (btv). sheep are considered the most susceptible hosts, while cattle, buffalo and goats serve as reservoirs. the viral pathogenesis of btv resulting in presence or absence of clinical disease among different hosts is not clearly understood. in the present study, transcriptome of sheep and goats peripheral blood mononuclear cells infected with btv-16 was exp ... | 2016 | 28003963 |
| ovarian transcriptomic study reveals the differential regulation of mirnas and lncrnas related to fecundity in different sheep. | mirnas and lncrnas, which represent one of the most highly expressed classes of ncrnas in development, are attracting increasing interest. a variety of regulators is considered to be implicated in sheep species with different fecundity. however, interactions between mirnas and lncrnas and changes in the expression of regulatory lncrnas in sheep fecundity have not yet been reported. to characterize the important roles of mirnas and lncrnas and elucidate their regulating networks in sheep prolific ... | 2016 | 27731399 |
| allelic variation of melanocortin-1 receptor locus in saudi indigenous sheep exhibiting different color coats. | this study was designed to characterize the dna polymorphisms of the melanocortin-1 receptor (mc1r) gene in indigenous saudi arabian sheep breeds exhibiting different color coats, along with individuals of the sawaknee breed, an exotic sheep imported from sudan. | 2016 | 27492350 |
| strategies for determining kinship in wild populations using genetic data. | knowledge of kin relationships between members of wild animal populations has broad application in ecology and evolution research by allowing the investigation of dispersal dynamics, mating systems, inbreeding avoidance, kin recognition, and kin selection as well as aiding the management of endangered populations. however, the assessment of kinship among members of wild animal populations is difficult in the absence of detailed multigenerational pedigrees. here, we first review the distinction b ... | 2016 | 27648229 |
| dietary resources shape the adaptive changes of cyanide detoxification function in giant panda (ailuropoda melanoleuca). | the functional adaptive changes in cyanide detoxification in giant panda appear to be response to dietary transition from typical carnivore to herbivorous bear. we tested the absorption of cyanide contained in bamboo/bamboo shoots with a feeding trial in 20 adult giant pandas. we determined total cyanide content in bamboo shoots and giant panda's feces, levels of urinary thiocyanate and tissue rhodanese activity using color reactions with a spectrophotometer. rhodanese expression in liver and ki ... | 2016 | 27703267 |
| mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an erk signalling-mediated mitochondria pathway. | mycoplasma ovipneumoniae (m. ovipneumoniae) is a species of mycoplasma bacteria that specifically infects sheep and goat, causing ovine infectious pleuropneumonia. however, the mechanism underlying the pathogen-host interaction between m. ovipneumoniae and airway epithelial cells is unknown. | 2016 | 27663303 |
| transcriptomic study on ovine immune responses to fasciola hepatica infection. | fasciola hepatica is not only responsible for major economic losses in livestock farming, but is also a major food-borne zoonotic agent, with 180 million people being at risk of infection worldwide. this parasite is sophisticated in manipulating the hosts' immune system to benefit its own survival. a better understanding of the mechanisms underpinning this immunomodulation is crucial for the development of control strategies such as vaccines. | 2016 | 27661612 |
| generation of biallelic knock-out sheep via gene-editing and somatic cell nuclear transfer. | transgenic sheep can be used to achieve genetic improvements in breeds and as an important large-animal model for biomedical research. in this study, we generated a talen plasmid specific for ovine mstn and transfected it into fetal fibroblast cells of sth sheep. mstn biallelic-ko somatic cells were selected as nuclear donor cells for scnt. in total, cloned embryos were transferred into 37 recipient gilts, 28 (75.7%) becoming pregnant and 15 delivering, resulting in 23 lambs, 12 of which were al ... | 2016 | 27654750 |
| regulation of blm nucleolar localization. | defects in coordinated ribosomal rna (rrna) transcription in the nucleolus cause cellular and organismal growth deficiencies. bloom's syndrome, an autosomal recessive human disorder caused by mutated recq-like helicase blm, presents with growth defects suggestive of underlying defects in rrna transcription. our previous studies showed that blm facilitates rrna transcription and interacts with rna polymerase i and topoisomerase i (top1) in the nucleolus. the mechanisms regulating localization of ... | 2016 | 27657136 |
| comparative transcriptome profiling of longissimus muscle tissues from qianhua mutton merino and small tail han sheep. | the qianhua mutton merino (qhmm) is a new sheep (ovis aries) variety with better meat performance compared with the traditional local variety small tail han (sth) sheep. we aimed to evaluate the transcriptome regulators associated with muscle growth and development between the qhmm and sth. we used rna-seq to obtain the transcriptome profiles of the longissimus muscle from the qhmm and sth. the results showed that 960 genes were differentially expressed (405 were up-regulated and 555 were down-r ... | 2016 | 27645777 |
| in silico analysis of the binding of anthelmintics to caenorhabditis elegansp-glycoprotein 1. | macrocyclic lactones (ml) are important anthelmintics used in animals and humans against parasite nematodes, but their therapeutic success is compromised by the spread of ml resistance. some abc transporters, such as p-glycoproteins (pgps), are selected and overexpressed in ml-resistant nematodes, supporting a role for some drug efflux proteins in ml resistance. however, the role of such proteins in ml transport remains to be clarified at the molecular level. recently, caenorhabditis elegans pgp ... | 2016 | 27746191 |
| orf virus 002 protein targets ovine protein s100a4 and inhibits nf-κb signaling. | orf virus (orfv), a member of parapoxvirus, has evolved various strategies to modulate the immune responses of host cells. the orfv-encoded protein orfv002, a regulator factor, has been found to inhibit the acetylation of nf-κb-p65 by blocking phosphorylation of nf-κb-p65 at ser(276) and also to disrupt the binding of nf-κb-p65 and p300. to explore the mechanism by which orfv002 regulates nf-κb signaling, the understanding of orfv002 potential binding partners in host cells is critical. in this ... | 2016 | 27679610 |
| general methods for evolutionary quantitative genetic inference from generalized mixed models. | methods for inference and interpretation of evolutionary quantitative genetic parameters, and for prediction of the response to selection, are best developed for traits with normal distributions. many traits of evolutionary interest, including many life history and behavioral traits, have inherently nonnormal distributions. the generalized linear mixed model (glmm) framework has become a widely used tool for estimating quantitative genetic parameters for nonnormal traits. however, whereas glmms ... | 2016 | 27591750 |
| sex differences in contaminant concentrations of fish: a synthesis. | a comparison of whole-fish polychlorinated biphenyl (pcb) and total mercury (hg) concentrations in mature males with those in mature females may provide insights into sex differences in behavior, metabolism, and other physiological processes. in eight species of fish, we observed that males exceeded females in whole-fish pcb concentration by 17 to 43 %. based on results from hypothesis testing, we concluded that these sex differences were most likely primarily driven by a higher rate of energy e ... | 2016 | 27594982 |
| expression of fbn1 during adipogenesis: relevance to the lipodystrophy phenotype in marfan syndrome and related conditions. | fibrillin-1 is a large glycoprotein encoded by the fbn1 gene in humans. it provides strength and elasticity to connective tissues and is involved in regulating the bioavailability of the growth factor tgfβ. mutations in fbn1 may be associated with depleted or abnormal adipose tissue, seen in some patients with marfan syndrome and lipodystrophies. as this lack of adipose tissue does not result in high morbidity or mortality, it is generally under-appreciated, but is a cause of psychosocial proble ... | 2016 | 27386756 |
| effects of early feeding on the host rumen transcriptome and bacterial diversity in lambs. | early consumption of starter feed promotes rumen development in lambs. we examined rumen development in lambs fed starter feed for 5 weeks using histological and biochemical analyses and by performing high-throughput sequencing in rumen tissues. additionally, rumen contents of starter feed-fed lambs were compared to those of breast milk-fed controls. our physiological and biochemical findings revealed that early starter consumption facilitated rumen development, changed the pattern of ruminal fe ... | 2016 | 27576848 |
| practical bias correction in aerial surveys of large mammals: validation of hybrid double-observer with sightability method against known abundance of feral horse (equus caballus) populations. | reliably estimating wildlife abundance is fundamental to effective management. aerial surveys are one of the only spatially robust tools for estimating large mammal populations, but statistical sampling methods are required to address detection biases that affect accuracy and precision of the estimates. although various methods for correcting aerial survey bias are employed on large mammal species around the world, these have rarely been rigorously validated. several populations of feral horses ... | 2016 | 27139732 |
| the identification of goat peroxiredoxin-5 and the evaluation and enhancement of its stability by nanoparticle formation. | an anticancer bioactive peptide (acbp), goat peroxiredoxin-5 (gprdx5), was identified from goat-spleen extract after immunizing the goat with gastric cancer-cell lysate. its amino acid sequence was determined by employing 2d nano-lc-esi-ltq-orbitrap ms/ms combined with mascot database search in the goat subset of the uniprot database. the recombinant gprdx5 protein was acquired by heterogeneous expression in escherichia coli. subsequently, the anti-cancer bioactivity of the peptide was measured ... | 2016 | 27074889 |
| abcb1 in pigs: molecular cloning, tissues distribution, functional analysis, and its effect on pharmacokinetics of enrofloxacin. | p-glycoprotein (p-gp) is one of the best-known atp-dependent efflux transporters, contributing to differences in pharmacokinetics and drug-drug interactions. until now, studies on pig p-gp have been scarce. in our studies, the full-length porcine p-gp cdna was cloned and expressed in a madin-darby canine kidney (mdck) cell line. p-gp expression was then determined in tissues and its role in the pharmacokinetics of oral enrofloxacin in pigs was studied. the coding region of pig abcb1 gene was 3,8 ... | 2016 | 27572343 |
| spata2-mediated binding of cyld to hoip enables cyld recruitment to signaling complexes. | recruitment of the deubiquitinase cyld to signaling complexes is mediated by its interaction with hoip, the catalytically active component of the linear ubiquitin chain assembly complex (lubac). here, we identify spata2 as a constitutive direct binding partner of hoip that bridges the interaction between cyld and hoip. spata2 recruitment to tnfr1- and nod2-signaling complexes is dependent on hoip, and loss of spata2 abolishes cyld recruitment. deficiency in spata2 exerts limited effects on gene ... | 2016 | 27545878 |
| genomic and expression analyses of tursiops truncatus t cell receptor gamma (trg) and alpha/delta (tra/trd) loci reveal a similar basic public γδ repertoire in dolphin and human. | the bottlenose dolphin (tursiops truncatus) is a mammal that belongs to the cetartiodactyla and have lived in marine ecosystems for nearly 60 millions years. despite its popularity, our knowledge about its adaptive immunity and evolution is very limited. furthermore, nothing is known about the genomics and evolution of dolphin antigen receptor immunity. | 2016 | 27528257 |
| genetic diversity and phylogenetic evolution of tibetan sheep based on mtdna d-loop sequences. | the molecular and population genetic evidence of the phylogenetic status of the tibetan sheep (ovis aries) is not well understood, and little is known about this species' genetic diversity. this knowledge gap is partly due to the difficulty of sample collection. this is the first work to address this question. here, the genetic diversity and phylogenetic relationship of 636 individual tibetan sheep from fifteen populations were assessed using 642 complete sequences of the mitochondrial dna d-loo ... | 2016 | 27463976 |
| effects of rice straw supplemented with urea and molasses on intermediary metabolism of plasma glucose and leucine in sheep. | an isotope dilution method using [u-(13)c]glucose and [1-(13)c]leucine (leu) was conducted to evaluate the effects of rice straw supplemented with urea and molasses (rsum-diet) on plasma glucose and leu turnover rates in sheep. nitrogen (n) balance, rumen fermentation characteristics and blood metabolite concentrations were also determined. four sheep were fed either mixed hay (mh-diet), or a rsum-diet with a crossover design for two 21 days period. feed allowance was computed on the basis of me ... | 2016 | 26949953 |
| myxoma virus m156 is a specific inhibitor of rabbit pkr but contains a loss-of-function mutation in australian virus isolates. | myxoma virus (myxv) is a rabbit-specific poxvirus, which is highly virulent in european rabbits. the attenuation of myxv and the increased resistance of rabbits following the release of myxv in australia is one of the best-documented examples of host-pathogen coevolution. to elucidate the molecular mechanisms that contribute to the restriction of myxv infection to rabbits and myxv attenuation in the field, we have studied the interaction of the myxv protein m156 with the host antiviral protein k ... | 2016 | 26903626 |
| spatio-temporal analysis of the genetic diversity of arctic rabies viruses and their reservoir hosts in greenland. | there has been limited knowledge on spatio-temporal epidemiology of zoonotic arctic fox rabies among countries bordering the arctic, in particular greenland. previous molecular epidemiological studies have suggested the occurrence of one particular arctic rabies virus (rabv) lineage (arctic-3), but have been limited by a low number of available samples preventing in-depth high resolution phylogenetic analysis of rabvs at that time. however, an improved knowledge of the evolution, at a molecular ... | 2016 | 27459154 |
| post-mortem corneal thickness measurements with a portable optical coherence tomography system: a reliability study. | the purpose of this study was to determine the repeatability and reproducibility of post-mortem central corneal thickness (cct) measurements by using a real-time, portable optical coherence tomography (oct) system on an animal model, and to prospectively evaluate the time-course of post-mortem changes in cct. forty-six ocular globes of sheep (ovis aries) were analyzed with a portable spectral-domain oct device by two operators at different postmortem intervals (pmis) as follows: immediately (i.e ... | 2016 | 27457021 |
| seeds, browse, and tooth wear: a sheep perspective. | while grazing as a selective factor towards hypsodont dentition on mammals has gained a lot of attention, the importance of fruits and seeds as fallback resources for many browsing ungulates has caught much less attention. controlled-food experiments, by reducing the dietary range, allow for a direct quantification of the effect of each type of items separately on enamel abrasion. we present the results of a dental microwear texture analysis on 40 ewes clustered into four different controlled di ... | 2016 | 27547337 |
| intra-amniotic candida albicans infection induces mucosal injury and inflammation in the ovine fetal intestine. | chorioamnionitis is caused by intrauterine infection with microorganisms including candida albicans (c.albicans). chorioamnionitis is associated with postnatal intestinal pathologies including necrotizing enterocolitis. the underlying mechanisms by which intra-amniotic c.albicans infection adversely affects the fetal gut remain unknown. therefore, we assessed whether intra-amniotic c.albicans infection would cause intestinal inflammation and mucosal injury in an ovine model. additionally, we tes ... | 2016 | 27411776 |
| whole-genome sequencing of native sheep provides insights into rapid adaptations to extreme environments. | global climate change has a significant effect on extreme environments and a profound influence on species survival. however, little is known of the genome-wide pattern of livestock adaptations to extreme environments over a short time frame following domestication. sheep (ovis aries) have become well adapted to a diverse range of agroecological zones, including certain extreme environments (e.g., plateaus and deserts), during their post-domestication (approximately 8-9 kya) migration and differ ... | 2016 | 27401233 |
| dataset of milk whey proteins of three indigenous greek sheep breeds. | the importance and unique biological traits, as well as the growing financial value, of milk from small greek ruminants is continuously attracting interest from both the scientific community and industry. in this regard the construction of a reference dataset of the milk of the greek sheep breeds is of great interest. in order to obtain such a dataset we employed cutting-edge proteomics methodologies to investigate and characterize, the proteome of milk from the three indigenous greek sheep bree ... | 2016 | 27508236 |
| towards evenly distributed grazing patterns: including social context in sheep management strategies. | background. a large proportion of natural grasslands around the world is exposed to overgrazing resulting in land degradation and biodiversity loss. although there is an increasing effort in the promotion of sustainable livestock management, rangeland degradation still occurs because animals' foraging behaviour is highly selective at different spatial scales. the assessment of the ecological mechanisms modulating the spatial distribution of grazing and how to control it has critical implications ... | 2016 | 27366650 |
| silicon: potential to promote direct and indirect effects on plant defense against arthropod pests in agriculture. | silicon has generally not been considered essential for plant growth, although it is well recognized that many plants, particularly poaceae, have substantial plant tissue concentrations of this element. recently, however, the international plant nutrition institute [ipni] (2015), georgia, usa has listed it as a "beneficial substance". this reflects that numerous studies have now established that silicon may alleviate both biotic and abiotic stress. this paper explores the existing knowledge and ... | 2016 | 27379104 |
| genome-wide detection of cnvs in chinese indigenous sheep with different types of tails using ovine high-density 600k snp arrays. | chinese indigenous sheep can be classified into three types based on tail morphology: fat-tailed, fat-rumped, and thin-tailed sheep, of which the typical breeds are large-tailed han sheep, altay sheep, and tibetan sheep, respectively. to unravel the genetic mechanisms underlying the phenotypic differences among chinese indigenous sheep with tails of three different types, we used ovine high-density 600k snp arrays to detect genome-wide copy number variation (cnv). in large-tailed han sheep, alta ... | 2016 | 27282145 |
| integrated analysis of the roles of long noncoding rna and coding rna expression in sheep (ovis aries) skin during initiation of secondary hair follicle. | initiation of hair follicle (hf) is the first and most important stage of hf morphogenesis. however the precise molecular mechanism of initiation of hair follicle remains elusive. meanwhile, in previous study, the more attentions had been paid to the function of genes, while the roles of non-coding rnas (such as long noncoding rna and microrna) had not been described. therefore, the roles of long noncoding rna(lncrna) and coding rna in sheep skin during the initiation of sheep secondary hf were ... | 2016 | 27276011 |
| ovarian proteomic study reveals the possible molecular mechanism for hyperprolificacy of small tail han sheep. | small tail han sheep is a widely bred farm animal in china which has attracted lots of attention due to their high prolificacy and year-round estrus. however, the molecular mechanism of its fecundity remains unrevealed. the fecb gene polymorphism has been found to be associated with the ovulation rate and litter size of sheep. in the present study, we constructed an itraq-based quantitative proteomics analysis to compare the ovarian proteomes of fecb+fecb+ genotype small tail han sheep ewes (han ... | 2016 | 27271055 |
| genomic analysis reveals depression due to both individual and maternal inbreeding in a free-living mammal population. | there is ample evidence for inbreeding depression manifested as a reduction in fitness or fitness-related traits in the focal individual. in many organisms, fitness is not only affected by genes carried by the individual, but also by genes carried by their parents, for example if receiving parental care. while maternal effects have been described in many systems, the extent to which inbreeding affects fitness directly through the focal individual, or indirectly through the inbreeding coefficient ... | 2016 | 27135155 |
| timing of population peaks of norway lemming in relation to atmospheric pressure: a hypothesis to explain the spatial synchrony. | herbivore cycles are often synchronized over larger areas than what could be explained by dispersal. in norway, the 3-4 year lemming cycle usually show no more than a one-year time lag between different regions, despite distances of up to 1000 km. if important food plants are forced to reallocate defensive proteins in years with high seed production, spatially synchronized herbivore outbreaks may be due to climate-synchronized peaks in flowering. because lemming peaks are expected to occur one y ... | 2016 | 27249449 |
| conditions during adulthood affect cohort-specific reproductive success in an arctic-nesting goose population. | variation in fitness between individuals in populations may be attributed to differing environmental conditions experienced among birth (or hatch) years (i.e., between cohorts). in this study, we tested whether cohort fitness could also be explained by environmental conditions experienced in years post-hatch, using 736 lifelong resighting histories of greenland white-fronted geese (anser albifrons flavirostris) marked in their first winter. specifically, we tested whether variation in age at fir ... | 2016 | 27257539 |
| erratum: a genome-wide association study identifies a genomic region for the polycerate phenotype in sheep (ovis aries). | 2016 | 27203473 | |
| density-dependent diel activity in stream-dwelling arctic charr salvelinus alpinus. | intraspecific competition plays a significant role in shaping how animals use and share habitats in space and time. however, the way individuals may modify their diel activity in response to increased competition has received limited attention. we used juvenile (age 1+) arctic charr salvelinus alpinus to test the prediction that individuals at high population density are more active and distribute their foraging activity over a greater portion of the 24-h cycle than individuals at low population ... | 2016 | 27247761 |
| transcriptome profile of one-month-old lambs' granulosa cells after superstimulation. | superstimulatory treatment of one-month-old lambs can achieve synchronous development of numerous growing follicles. however, these growing follicles cannot complete maturation and ovulation. oocyte maturation and competence are acquired during follicular development, in which granulosa cells play an essential role. | 2016 | 27189640 |
| genetic characterization of a novel mutant of peste des petits ruminants virus isolated from capra ibex in china during 2015. | peste des petits ruminants virus (pprv) is the causative agent of peste des petits ruminants (ppr). the spread of ppr often causes severe economic losses. therefore, special attention should be paid to the surveillance of ppr emergence, spread, and geographic distribution. here we describe a novel mutant of pprv china/xjbz/2015 that was isolated from capra ibex in xinjiang province in china 2015. the sequence analysis and phylogenetic assessment indicate that china/xjbz/2015 belongs to lineage i ... | 2016 | 26998489 |
| potential foraging decisions by a desert ungulate to balance water and nutrient intake in a water-stressed environment. | arid climates have unpredictable precipitation patterns, and wildlife managers often provide supplemental water to help desert ungulates endure the hottest, driest periods. when surface water is unavailable, the only source of water for ungulates comes from the forage they consume, and they must make resourceful foraging decisions to meet their requirements. we compared two desert bighorn sheep (ovis canadensis nelsoni) populations in arizona, usa: a treatment population with supplemental water ... | 2016 | 26894504 |
| expression profiling and functional characterization of mir-192 throughout sheep skeletal muscle development. | micrornas (mirnas) are evolutionarily conserved, small, non-coding rnas that have emerged as key regulators of myogenesis. here, we examined the mirna expression profiles of developing sheep skeletal muscle using a deep sequencing approach. we detected 2,396 mirnas in the sheep skeletal muscle tissues. of these, mir-192 was found to be up-regulated in prenatal skeletal muscle, but was down-regulated postnatally. mir-192 expression also decreased during the myogenic differentiation of sheep satel ... | 2016 | 27452271 |
| genomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the himalayas. | sheep has successfully adapted to the extreme high-altitude himalayan region. to identify genes underlying such adaptation, we genotyped genome-wide single nucleotide polymorphisms (snps) of four major sheep breeds living at different altitudes in nepal and downloaded snp array data from additional asian and middle east breeds. using a di value-based genomic comparison between four high-altitude and eight lowland asian breeds, we discovered the most differentiated variants at the locus of fgf-7 ... | 2016 | 27444145 |
| an integrated analysis of microrna and mrna expression profiles to identify rna expression signatures in lambskin hair follicles in hu sheep. | wave patterns in lambskin hair follicles are an important factor determining the quality of sheep's wool. hair follicles in lambskin from hu sheep, a breed unique to china, have 3 types of waves, designated as large, medium, and small. the quality of wool from small wave follicles is excellent, while the quality of large waves is considered poor. because no molecular and biological studies on hair follicles of these sheep have been conducted to date, the molecular mechanisms underlying the forma ... | 2016 | 27404636 |
| in silico identification of essential proteins in corynebacterium pseudotuberculosis based on protein-protein interaction networks. | corynebacterium pseudotuberculosis (cp) is a gram-positive bacterium that is classified into equi and ovis serovars. the serovar ovis is the etiological agent of caseous lymphadenitis, a chronic infection affecting sheep and goats, causing economic losses due to carcass condemnation and decreased production of meat, wool, and milk. current diagnosis or treatment protocols are not fully effective and, thus, require further research of cp pathogenesis. | 2016 | 27814699 |
| three thousand years of continuity in the maternal lineages of ancient sheep (ovis aries) in estonia. | although sheep (ovis aries) have been one of the most exploited domestic animals in estonia since the late bronze age, relatively little is known about their genetic history. here, we explore temporal changes in estonian sheep populations and their mitochondrial genetic diversity over the last 3000 years. we target a 558 base pair fragment of the mitochondrial hypervariable region in 115 ancient sheep from 71 sites in estonia (c. 1200 bc-ad 1900s), 19 ancient samples from latvia, russia, poland ... | 2016 | 27732668 |
| extreme wildlife declines and concurrent increase in livestock numbers in kenya: what are the causes? | there is growing evidence of escalating wildlife losses worldwide. extreme wildlife losses have recently been documented for large parts of africa, including western, central and eastern africa. here, we report extreme declines in wildlife and contemporaneous increase in livestock numbers in kenya rangelands between 1977 and 2016. our analysis uses systematic aerial monitoring survey data collected in rangelands that collectively cover 88% of kenya's land surface. our results show that wildlife ... | 2016 | 27676077 |
| genetic variation of goat interferon regulatory factor 3 gene and its implication in goat evolution. | the immune systems are fundamentally vital for evolution and survival of species; as such, selection patterns in innate immune loci are of special interest in molecular evolutionary research. the interferon regulatory factor (irf) gene family control many different aspects of the innate and adaptive immune responses in vertebrates. among these, irf3 is known to take active part in very many biological processes. we assembled and evaluated 1356 base pairs of the irf3 gene coding region in domesti ... | 2016 | 27598391 |
| prevalence and scanning electron microscopic identification of anoplocephalid cestodes among small ruminants in senegal. | this study was undertaken to determine the prevalence of anoplocephalid cestodes in sheep and goats in senegal. intestines of 462 sheep and 48 goats were examined; 47.4% of sheep and 6.2% of goats were infected. the species identified and their prevalence were, among sheep, avitellina centripunctata 38.7%, moniezia expansa 15.4%, stilesia globipunctata 16.7%, and thysaniezia ovilla 0.4%. among goats, they were m. expansa 6.2% and t. ovilla 2.1%. the prevalence of all species was not statisticall ... | 2016 | 27597893 |
| late pleistocene/early holocene migratory behavior of ungulates using isotopic analysis of tooth enamel and its effects on forager mobility. | zooarchaeological and paleoecological investigations have traditionally been unable to reconstruct the ethology of herd animals, which likely had a significant influence on the mobility and subsistence strategies of prehistoric humans. in this paper, we reconstruct the migratory behavior of red deer (cervus elaphus) and caprids at the pleistocene-holocene transition in the northeastern adriatic region using stable oxygen isotope analysis of tooth enamel. the data show a significant change in δ18 ... | 2016 | 27275784 |
| oxygen isotope in archaeological bioapatites from india: implications to climate change and decline of bronze age harappan civilization. | the antiquity and decline of the bronze age harappan civilization in the indus-ghaggar-hakra river valleys is an enigma in archaeology. weakening of the monsoon after ~5 ka bp (and droughts throughout the asia) is a strong contender for the harappan collapse, although controversy exists about the synchroneity of climate change and collapse of civilization. one reason for this controversy is lack of a continuous record of cultural levels and palaeomonsoon change in close proximity. we report a hi ... | 2016 | 27222033 |
| reasoning by exclusion in the kea (nestor notabilis). | reasoning by exclusion, i.e. the ability to understand that if there are only two possibilities and if it is not a, it must be b, has been a topic of great interest in recent comparative cognition research. many studies have investigated this ability, employing different methods, but rarely exploring concurrent decision processes underlying choice behaviour of non-human animals encountering inconsistent or incomplete information. here, we employed a novel training and test method in order to per ... | 2016 | 27209174 |
| evaluation of risk factors for peste des petits ruminants virus in sheep and goats at the wildlife-livestock interface in punjab province, pakistan. | peste des petits ruminants virus (pprv) is causing infectious disease with high morbidity and mortality rate in domestic and wild small ruminants of pakistan with valuable economical losses. the present study was carried out to investigate risk factors of pprv in domestic small ruminants which were present in the vicinity of wildlife parks. a total of 265 sera samples (27 wild ruminants and 238 domesticated small ruminants) from apparently healthy animals from two different wildlife parks were c ... | 2016 | 27294134 |
| the goat (capra hircus) mammary gland mitochondrial proteome: a study on the effect of weight loss using blue-native page and two-dimensional gel electrophoresis. | seasonal weight loss (swl) is the most important limitation to animal production in the tropical and mediterranean regions, conditioning producer's incomes and the nutritional status of rural communities. it is of importance to produce strategies to oppose adverse effects of swl. breeds that have evolved in harsh climates have acquired tolerance to swl through selection. most of the factors determining such ability are related to changes in biochemical pathways as affected by swl. in this study, ... | 2016 | 27031334 |
| combining untargeted and targeted proteomic strategies for discrimination and quantification of cashmere fibers. | cashmere is regarded as a specialty and luxury fiber due to its scarcity and high economic value. for fiber quality assessment, it is technically very challenging to distinguish and quantify the cashmere fiber from yak or wool fibers because of their highly similar physical appearance and substantial protein sequence homology. to address this issue, we propose a workflow combining untargeted and targeted proteomics strategies for selecting, verifying and quantifying biomarkers for cashmere texti ... | 2016 | 26789629 |
| human expansion precipitates niche expansion for an opportunistic apex predator (puma concolor). | there is growing recognition that developed landscapes are important systems in which to promote ecological complexity and conservation. yet, little is known about processes regulating these novel ecosystems, or behaviours employed by species adapting to them. we evaluated the isotopic niche of an apex carnivore, the cougar (puma concolor), over broad spatiotemporal scales and in a region characterized by rapid landscape change. we detected a shift in resource use, from near complete specializat ... | 2016 | 28008961 |