| blood pressure and metabolic effects of adrenocorticotrophin after administration to pregnant sheep. | | 1978 | 212499 |
| virus-replicating t cells in the immune response of mice. ii. characterization of t cells capable of replicating vesicular stomatitis virus. | immunocytological properties of the splenic t cell (tv) which develop into virus plaque-forming cells in response to the antigenic challenge in vitro were investigated in relation to the properties of helper t cells and suppressor t cells in antibody response. tv was observed in spleen around 1 wk after the intravenous injection of mice with 10(7) sheep erythrocytes. this contrasted with the finding that both helper t cells and suppressor t cells developed as early as 3 days after the immunizati ... | 1978 | 212507 |
| virus-replicating t cells in the immune response of mice. iii. role of vesicular stomatitis virus-replicating t cells in the antibody response. | the functional role of the t cell (tv) which can replicate vesicular stomatitis virus (vsv) on activation by the antigen was investigated in antibody response in vitro. by the inoculation of vsv into the culture, marked augmentation of antibody response to sheep erythrocytes (srbc) was observed in the culture of spleen cells taken more than 3 days after the immunization with srbc, suggesting that the vsv-susceptible suppressor cells were included in these spleen cells and the activity was elimin ... | 1978 | 212508 |
| role of brain monoamines and histamine in regulation of anterior pituitary secretion. | | 1978 | 212770 |
| peptides in the brain: the new endocrinology of the neuron. | | 1978 | 212832 |
| [serological diagnosis of bovine leukosis using the agar gel diffusion test]. | experiments were carried out to use the agar gel diffusion test (agdt) in the study of bovine leukosis. an antigen was obtained from flk-24 cells with a latent leukosis virus infection, which proved specific and suitable for agdt. a total of 134 serum samples were examined applyig agdt, for the presence of specific antibodies, originating from cattle of herds in which leukosis had been hematologically demonstrated. investigated were also 56 sera of animals presenting an unknown clinical picture. ... | 1978 | 212862 |
| [comparison of various serological methods for the demonstration of maedi/visna virus antibodies]. | | 1978 | 212904 |
| experimental visna in foetal icelandic sheep. | | 1978 | 213455 |
| resistance of visna virus to interferon. | visna is a slow infection of sheep caused by a retrovirus. the persistence of virus despite the immune response of the host is best explained by restricted genetic expression of the virus and consequently prolonged periods of residence inside cells. the purpose of this investigation was to determine whether the restriction in genetic expression of visna virus is mediated by interferon. sheep interferon induced by polyriboinosinic-polyribocytidylic acid in fetal lambs inhibited the growth of herp ... | 1978 | 213502 |
| observations on the effect of different methods of processing on the bacterial contaminants of bovine and ovine tripe. | a comparison was made of the multiplication of bacteria in specimens of tripe processed in different ways. there was little difference in this respect between commercial tripe as offered for purchase to the consumer and rough and scraped tripe incubated at 30 degrees c for 24 h. tripe stored at 4 degrees c or in acetic acid (ph 4) at 30 degrees c for 24 h, however, had appreciably fewer bacteria. | 1978 | 213753 |
| evidence for h2o2 mediating the irreversible action of acetylenic inhibitors of prostaglandin biosynthesis. | oxidizing intermediates formed during prostaglandin biosynthesis can be detected by ferrocytochrome c and epinephrine. different intermediates were responsible for the oxidative colorimetric changes with epinephrine and ferrocytochrome c, and submicromolar amounts of oxidant were detectable. catalase diminished the absorbance change with epinephrine, but it did not stop the conversion of arachidonate to prostaglandins. this result indicates that small amounts of h2o2 were formed when producing t ... | 1978 | 213797 |
| [culture of the sheep pox virus on cells: application to vaccination and immunity control]. | | 1978 | 213823 |
| immunopathogenesis of visna. a slow virus disease of the central nervous system. | | 1978 | 213925 |
| intranasal tumor of the ethmoid olfactory mucosa in sheep. | intranasal tumors (papillary adenomas or adenocarcinomas) of the ethmoid olfactory mucosa of sheep were investigated by light and electron microscopy. the fine structure of the tumor cells was characterized by the presence of numerous secretory granules. viral particles, which were morphologically similar to a visna-maedi virus, were detected in all tumor tissues and in 3 of 4 cultures examined. the particles (about 97 nm) had an eccentrically located electron-dense core and numerous spikes on t ... | 1978 | 213993 |
| [is camp an intermediate in the releasing action of gn-rh? (proceedings) (author's transl)]. | | 1978 | 214015 |
| the cerebrospinal fluid in visna, a slow viral disease of sheep. | the temporal development of the csf abnormalities was examined in visna, a chronic neurological disease caused by a persistent viral infection of sheep. two types of changes were observed. during the first 30 to 40 days there was an intense mononuclear pleocytosis associated with high protein and proportionately elevated igg. later, there was a persistent slight pleocytosis associated with mildly elevated protein which had an increased proportion of igg and antivisna antibody. these later cellul ... | 1978 | 214025 |
| sedimentation characteristics of the scrapie agent from murine spleen and brain. | sedimentation profiles of the scrapie agent in extracts of murine spleen and brain were determined by analytical differential centrifugation. infectivity profiles of the agent from the two tissues were similar. sedimentation of the agent was not substantially altered by detergent treatment with sodium deoxycholate. in the presence of detergent, centrifugation at an omega2t value of 3.0 x 1010 rad2/s in a fixed-angle rotor sedimented 90% of the agent. comparative studies with radioisotopically la ... | 1978 | 214106 |
| tryptophan fluorescence of (na+ + k+)-atpase as a tool for study of the enzyme mechanism. | 1. the protein fluorescence intensity of (na+ + k+)-atpase is enhanced following binding of k+ at low concentrations. the properties of the response suggest that one or a few tryptophan residues are affected by a conformational transition between the k-bound form e2 . (k) and a na-bound form e1 . na. 2. the rate of the conformational transition e2 . (k) leads to e . na has been measured with a stopped-flow fluorimeter by exploiting the difference in fluorescence of the two states. in the absence ... | 1978 | 214132 |
| biology of the human malignant lymphomas. iv. functional characterization of ten diffuse histiocytic lymphoma cell lines. | ten consecutive diffuse histiocytic lymphoma (dhl) cell lines established in our laboratory were studied for the presence of epstein-barr virus (ebv) genomes, lysozyme, nonspecific esterase and other cytochemical reactions, phagocytic activity, cytoplasmic immunoglobulin light and heavy chains, and surface receptors to sheep erythrocytes, complement, and the fc fragment of immunoglobulin. in agreement with previous studies performed on biopsy specimens, our results indicate that the diffuse hist ... | 1978 | 214220 |
| blood pressure and metabolic effects of acth in anephric sheep. | 1. acth (80--100 iu/day) was administered to seven sheep for 3--5 days following bilateral nephrectomy. 2. acth treatment produced rises in mean arterial pressure in anephric sheep similar to those observed in intact animals. following withdrawal of acth, blood pressure declined over the following 72 h. 3. acth produced a transient fall in plasma potassium and anephric sheep, suggesting an internal redistribution mechanism for this ion. 4. these studies demonstrate that adrenal steroid hormones ... | 1978 | 214264 |
| effect of copper and zinc status on susceptibility to cadmium intoxication. | the effects of dietary cadmium on copper and zinc metabolism in animals are described. emphasis is given to situations involving chronic exposure to low levels of cadmium, to the identification of population groups most at risk, and to the protective effect of dietary supplementation with copper and zinc. the mechanism of the interaction between the metals and the involvement of metallothionein are discussed. | 1978 | 214298 |
| effect of temperature on phosphorylation and ouabain binding to n-ethylmaleimide-treated (na+, k+)-atpase. | | 1978 | 214431 |
| heterogeneity of amino acid sequence in hippopotamus cytochrome c. | the amino acid sequences of chymotryptic and tryptic peptides of hippopotamus amphibius cytochrome c were determined by a recent modification of the manual edman sequential degradation procedure. they were ordered by comparison with the structure of the hog protein. the hippopotamus protein differs in three positions: serine, alanine, and glutamine replace alanine, glutamic acid, and lysine in positions 43, 92, and 100, respectively. since the artiodactyl suborders diverged in the mid-eocene som ... | 1978 | 214435 |
| virus mutation during 'slow infection': temporal development and characterization of mutants of visna virus recovered from sheep. | visna virus could be recovered from peripheral blood leukocytes of sheep for years after intracerebral inoculation. viruses recovered from sheep prior to and several months after development of antibody were antigenically identical to the parental strain used for inoculation. subsequently, mutant viruses which were not neutralized by the animals' sera were obtained. longitudinal studies of leukocyte viruses collected from two infected sheep showed that more than one strain of virus could co-exis ... | 1978 | 214521 |
| renin-like (angiotensinogenase) activity in human eccrine sweat. | the presence of renin or renin-like activity (rla) was demonstrated in human eccrine sweat incubated with purified sheep angiotensinogen, using rat bioassay and angiotensin i radioimmunoassay. following cholinergic stimulation, sweat rla was found to range between 0 (unmeasurable) and 266 ng/ml.h, i.e. rla-values of sweat can be about 10 times higher than those of plasma. therefore, renin synthesis in sweat glands could be assumed. rla following activation of beta-adrenergic receptors by the adm ... | 1978 | 214751 |
| the distribution of antibodies to border disease virus among sheep in england and wales. | within a sample of the sheep population of england and wales, 10.8 per cent of 3506 individuals had immunodiffusion test antibody to bovine viral diarrhoea mucosal disease virus antigen. there were marked differences between various geographical regions in the proportion of sheep with antibody, and border disease may be more common in some areas than disease records indicate. | 1978 | 214835 |
| [experimental parainfluenza in lambs]. | | 1978 | 214932 |
| purification, assay, and kinetic properties of sheep liver pyridoxal kinase. | | 1978 | 215056 |
| synthesis of coreless, probably defective virus particles in cell cultures infected with rotaviruses. | pk-15 cells infected with pig and lamb rotavirus strains which were not adapted to serial growth in cell cultures were examined by electron microscopy. a major difference between virus morphogenesis in the initial passage in pk-15 cells and in intestinal epithelial cells was the generation of large numbers of coreless virus particles in pk-15 cells. the numbers of coreless particles increased with increasing multiplicity of infection. infectious virus was synthesized in pk-15 cells, but a variab ... | 1978 | 215104 |
| pathology of ovine foetus infection with bluetongue virus. | | 1978 | 215253 |
| the effect of corticotrophin (acth) administration on the pressor action of angiotensin ii, noradrenaline and tyramine in sheep. | 1. pressor responses to angiotensin ii, noradrenaline and tyramine were examined in sheep prior to and during the development of corticotrophin-induced hypertension. 2. pressor responses to angiotensin ii amide did not change with corticotrophin (acth) administration. small significant increases in pressor responses to noradrenaline occurred at low doses only (0.27 and 1.06 mumol/h). significant increases in response to tyramine occurred after 24h of acth administration, but were not maintained ... | 1978 | 215361 |
| receptors for follicle-stimulating hormone and human chorionic gonadotrophin in the ovine follicle: changes in binding with atresia in vitro [proceedings]. | | 1978 | 215694 |
| ovine placental lactogen in maternal and foetal plasma in sheep [proceedings]. | | 1978 | 215695 |
| the use of cattle to protect sheep from bluetongue infection. | studies on the host preferences of culicoides imicola, the vector of bluetongue virus in south africa, are reviewed. there is agreement that this species prefers to feed on cattle but will also feed on other bovidae and sheep. over a seven year period cattle kept near sheep on a natal farm appear to have appreciably reduced the incidence of bluetongue in the sheep. in addition to immunization this "decoy" approach is therefore recommended to assist in the protection of stock from insect borne di ... | 1978 | 215768 |
| [infectious causes of perinatal mortalities in ruminants (author's transl)]. | the advantages and disadvantages of the different diagnostic techniques e.g. pathological and microbiological studies, immunoglobulin and specific antibody determinations and fluorescent antibody studies in relation to these mortalities are discussed. the most important pathological lesions in the placentas and foetuses are described. | 1978 | 215769 |
| modification of immune response in nude mice infected with mouse hepatitis virus. | in nude mice experimentally infected with mouse hepatitis virus (mhv), the numbers of early and later plaque forming cells (pfc) to sheep red blood cells (srbc) generated in the spleen were 7 to 20 times and 2 to 163 times, respectively, greater than those in non-infected nude mice, when srbc were given at day 0 to day 21 postinfection. splenic theta-positive lymphocytes in infected nude mice were shown to increase only at day 10 or more postinfection. pfc response to bacterial lipopolysaccharid ... | 1978 | 215883 |
| detection of viral sequences of low reiteration frequency by in situ hybridization. | the sensitivity of in situ hybridization has been increased at least 10-fold by hybridizing in cdna excess, by increasing the diffusion of the cdna through the cells, by hybridizing at optimum temperature, and by stabilizing hybrids during autoradiography. saturation of intracellular rna with [3h]cdna has been achieved. the assay is quantitative. in situ hybridization has been used to detect and quantitate visna virus rna in infected cells. by using [3h]cdna with specific activity of 2 x 10(8) d ... | 1978 | 216014 |
| parturition in rodents. | | 1978 | 216119 |
| hormonal control of parturition in the sheep and goat. | | 1978 | 216120 |
| initiation of parturition in the human female. | | 1978 | 216122 |
| [toxicologic and biochemical study of the diagnosis of cadmium-related damage in sheep]. | | 1978 | 216190 |
| bluetongue and epizootic hemorrhagic disease viruses: their relationship to wildlife species. | | 1978 | 216247 |
| canine plasma cortisol (hydrocortisone) measured by radioimmunoassay: clinical absence of diurnal variation and results of acth stimulation and dexamethasone suppression tests. | a radioimmunoassay for plasma cortisol (hydrocortisone) was developed and validated for sensitivity, specificity, accuracy, precision, and parallelism. steroids were extracted with ethyl ether, and cortisol was purified by gel column chromatography prior to assay. [1,2-3h] cortisol and a commercially available sheep antibody to cortisol-21-hemisuccinate were used. free steriods were separated from bound steroids by centrifugation after adsorption to dextran-coated charcoal. plasma cortisol was m ... | 1978 | 216290 |
| ulcerative diseases of animals with an infectious etiology. | the oral lesions of five viral diseases of cattle are compared. two of the diseases, foot-and-mouth disease and vesicular stomatitis, cause vesicles, and rinderpest, bovine virus diarrhea and malignant catarrhal fever produce sharply demarcated erosive lesions. gross lesions of different diseases appear similar: however, histologically, there are subtle differences in the development of the lesions. | 1978 | 216786 |
| infectivity tests of secretions and excretions from cattle infected with bovine leukemia virus. | secretions and excretions from cattle with persistent bovine leukemia virus (blv) infections were tested for blv by inoculation into sheep. development of antibody to the blv glycoprotein antigen and reisolation of virus from peripheral blood leukocytes were the two criteria used to confirm infection in test sheep. the virus was detected in milk from 4 of 6 cows examined and in colostrum from 1 of 4 cows examined. the virus was not detected in semen from 8 bulls or in nasal secretions, saliva, a ... | 1979 | 216837 |
| exaggerated natriuresis in acth hypertension in sheep. | adrenocorticotrophic hormone (acth) administration to sheep (100 iu/day) produces a sustained increase in arterial pressure within 24 h. the effect of acth on excretion of an intravenous saline load was tested in 8 adult cross-bred merino ewes. a significant diuretic response to saline loading was found after 24 h but no increased natriuresis. on days 3 and 6, diuretic and natriuretic responses were both significantly increased. steroid-induced sodium retention may have abolished the natriuretic ... | 1978 | 216942 |
| electron spin resonance studies on interaction of complement proteins with erythrocyte membranes. | sheep erythrocytes have been spin labeled with 5-, 12-, and 16-nitroxystearic acid in order to investigate complement-induced changes in the physical state of the lipid bilayer. formation of osmotic lesions in the membrane causes an increase in the fluidity of the membrane which overcomes the decrease in membrane fluidity caused by the interaction of the complement proteins. a decrease in membrane fluidity is observed only when complement-lysed membranes are resealed or when complement proteins ... | 1978 | 217005 |
| [formation of immunity in sheep inoculated with varying doses of the foot-and-mouth disease virus 0194]. | | 1979 | 217141 |
| [residues of the antifluke preparations, dovenix and acredist, in sheep milk and their effect on the biochemical blood indices of sheep]. | the excretion of residual amounts of acedist and dovenix with the milk of sheep that had been treated with these preparations was established on the 24th, resp., up to the 27th day following treatment, though their presence in the milk was found to drop sharply after the third day. the treatment with acedist led to a dependable decrease (p less than 0.05) in the hemoglobin amount, the total protein, and the erythrocyte count, and a dependable increase (p less than 0.05) in the leukocyte count. t ... | 1978 | 217146 |
| dissociation of the mitogenic and rosette-forming effects of pha to stimulators of cyclic amp levels. | the mitogenic and the spontaneous e-rosette stimulating effects of phytohaemagglutinin (pha) were studied by examining their responses to an elevation of intracellular camp induced by pretreatment with aminophylline, adrenaline or pge1 or by adding exogenous camp. elevation of the intracellular camp levels of lymphocytes was found inhibitory to the mitogenic effect of pha, while leaving the increased early and stable spontaneous rosette formation unaffected. it also failed to depress the pha-ind ... | 1978 | 217221 |
| hypervitaminosis d in sheep. an experimental study. | | 1978 | 217259 |
| bluetongue virus infection in australia. | | 1978 | 217325 |
| bluetongue virus infection in australia. | | 1978 | 217326 |
| experimental infection of culicoides with a bluetongue virus isolated in australia. | | 1978 | 217328 |
| partial reversal of the acetaldehyde and butyraldehyde oxidation reactions catalysed by aldehyde dehydrogenases from sheep liver. | in the presence of acetic anhydride or butyric anhydride, liver aldehyde dehydrogenases catalyse the oxidation of nadh at ph 7.0 and 25 degrees c. the maximum velocities and michaelis constants for nadh at saturating anhydride concentrations are independent of which anhydride is used, the values being v'max. = 12 min-1 and km for nadh = 9 micrometer for the mitochondrial enzyme and v'max = 25 min-1 and km for nadh = 20 micrometer for the cytoplasmic enzyme. substitution of [4a-2h]nadh for nadh r ... | 1978 | 217349 |
| kinetic properties of aldehyde dehydrogenase from sheep liver mitochondria. | the kinetics of the nad+-dependent oxidation of aldehydes, catalysed by aldehyde dehydrogenase purified from sheep liver mitochondria, were studied in detail. lag phases were observed in the assays, the length of which were dependent on the enzyme concentration. the measured rates after the lag phase was over were directly proportional to the enzyme concentration. if enzyme was preincubated with nad+, the lag phase was eliminated. double-reciprocal plots with aldehyde as the variable substrate w ... | 1978 | 217355 |
| effect of prostaglandin f2alpha on the number of lh receptors in ovine corpora lutea. | | 1978 | 217461 |
| validation of methods and quantification of luteal receptors for lh throughout the estrous cycle and early pregnancy in ewes. | | 1978 | 217463 |
| a simple, rapid syncytial-inhibition test for antibodies to bovine leukemia virus. | cocultivation of equal numbers of cells from a fetal lamb kidney line infected with bovine leukemia virus and african green monkey (vero) cells results in the rapid production of syncytia. the effect was blocked or inhibited by serum containing antibodies to bovine leukemia virus. a serological test based on syncytial inhibition was compared to the agar gel immunodiffusion test and the modified direct complement fixation test for the detection of bovine leukemia virus antibodies in sera from leu ... | 1978 | 217507 |
| effect of ovine prolactin on serum somatomedin bioactivity in hypophysectomized female rats. | ovine prolactin (oprl) increased serum somatomedin (sm) bioactivity in hypophysectomized female rats. acth, in small but not large doses, augmented this oprl effect. these results suggest that in the female rat prl may regulate sm production. adrenal factors may variably modulate sm production or serum sm bioactivity. | 1978 | 217513 |
| distribution of binding sites for ovine placental lactogen in the sheep. | | 1978 | 217597 |
| failure of angiotensin ii to inhibit corticotropin-stimulated cortisol secretion. | in view of the reported inhibitory effect of angiotensin ii on cortisol secretion in human subjects, the effect of local angiotensin infusions on steroid secretion maintained by acth was examined by using sheep with cervical autotransplanted adrenal glands. during sustained submaximal stimulation by exogenous acth (40--80 microunit/min), the addition of local infusions of angiotensin ii (1.6--160.0 ng/min) caused increased aldosterone and smaller increments in cortisol secretion in most experien ... | 1978 | 217621 |
| catecholamine-induced stimulation of progesterone and adenosine 3',5'-monophosphate production by dispersed ovine luteal cells. | | 1978 | 217645 |
| effect of chronic trypanosoma brucei infection on the course of louping ill virus infection in mice. | the course of louping-ill virus infection in mice chronically infected with trypanosoma brucei was investigated. the mean survival time of mice infected with virus alone was 10.2 days, compared to 12.8 days in mice infected with t. brucei 14 days before virus challenge, and the cumulative mortality in the two groups was 62.5 and 90%, respectively. virus was only inconsistently detected in the blood of mice given virus alone but was regularly present at high titers for up to 12 days in the blood ... | 1979 | 217825 |
| a pigeon crop sac radioreceptor assay for prolactin. | ovine prolactin, labelled with 125i by either lactoperoxidase or a mild chloramine t method, bound to receptors from the pigeon crop sac mucosa cells of prolactin-injected pigeons. binding was demonstrated in a crude homogenate of mucosal cells removed from the crop by scraping and in a subcellular fraction in which 5'-nucleotidase activity was enhanced two- to threefold. the binding was specific, dependent on time, temperature and the concentration of receptors and had a dissociation constant o ... | 1978 | 217943 |
| the metabolic and endocrine effects of circulating catecholamines in fetal sheep. | 1. adrenaline and noradrenaline have been infused into the fetal sheep to produce plasma concentrations comparable to those seen during hypoxia. the effects have been compared with those of isoprenaline and methoxamine and the sensitivity to beta- and alpha-adrenergic antagonists has been followed. 2. adrenaline caused an alpha-mediated increase in blood glucose that is associated with a fall in plasma insulin concentration. it also caused a beta-mediated increase in plasma lactate, free fatty a ... | 1978 | 217988 |
| the identification and monitoring of cell line specificity. | | 1978 | 218226 |
| the 1977 outbreak of bluetongue in cyprus. | bluetongue outbreaks, ranging from isolated cases to widespread infections, have occurred in cyprus in about half of the years since 1924. serious outbreaks occurred in 1924, 1939, 1943, 1946, 1951, and 1965. most cases occurred in september, october, november and december. the last outbreak prior to 1977 was in 1969. virus types isolated in the past were types 3 and 4. in 1977 the outbreak was serious, affecting 13.1 per cent of 27,837 in-contact sheep; these were scattered in 303 flocks of 65 ... | 1978 | 218327 |
| the effect of vaccination on titres of antibody to rotavirus in colostrum and milk. | the continued presence of antibody to rotavirus in the gut of the neonatal ruminant has been shown to be important in protecting against clinical disease associated with rotavirus infections. this effect is dependant upon the amount of antibody included in the diet. titres of antibody to rotavirus may be relatively high in ruminant mammary secretions immediately after parturition but decline rapidly over the course of the first day. the influence of vaccination on the titres of antibody, the con ... | 1978 | 218490 |
| the influence of colostrum on neonatal rotaviral infections. | | 1978 | 218491 |
| investigation of the subcellular distribution of cyclic-amp phosphodiesterase in rat hepatocytes, using a rapid immunological procedure for the isolation of plasma membrane. | the distribution of cyclic-amp phosphodiesterase was investigated in subcellular fractions prepared from homogenates of rat liver or isolated hepatocytes. when measured at 1 mm or 1 microm substrate concentration, approx. 35% or 50%, respectively, of enzyme activity was particulate. the soluble activity appeared to be predominantly a 'high km' form, whereas the particulate activity had both 'high km' and 'low km' components. the recovery of cyclic-amp phosphodiesterase was measured using 1 micro ... | 1979 | 218638 |
| the occurrence of antibodies to parainfluenza3 virus in sera of some domestic animals of the sudan. | | 1979 | 218672 |
| humoral antibody response to bovine leukemia virus infection in cattle and sheep. | in this study, 345 cattle from 7 herds with a history of lymphosarcoma were tested for antibody to blv antigens by three serological methods, namely immunodiffusion using a bovine leukemia virus glycoprotein with a molecular weight of 60,000 as antigen, and radioimmunoassay using a bovine leukemia virus glycoprotein with a molecular weight of 60,000 and a bovine leukemia virus protein with a molecular weight of 24,000 as antigen. the three tests under comparison agreed for 335 animals, 240 being ... | 1979 | 218722 |
| small intestinal morphology and epithelial cell kinetics in lamb rotavirus infections. | morphologic changes in the small intestine of rotavirus-infected gnotobiotic lambs were investigated by measurement of villi and crypts in histologic sections of jejunum, midgut, and posterior ileum. in midgut, villus atrophy developed within 12 hr of infection and was apparent until 72 hr after infection. crypt hypertrophy was evident from 42 hr after infection until the end of the observation period (6 days after infection). changes in posterior ileum were similar in extent, but jejunal change ... | 1979 | 218864 |
| enzymatic methyl esterification of pituitary polypeptides. | methyl esterification of pituitary polypeptides by protein methylase ii (s-adenosylmethionine:protein-carboxyl o-methyltransferase, ec. 2.1.1.24) has been investigated. ovine lutropin and adrenocorticotropin (alpha1-39-acth) were found to be good methyl acceptor substrates, followed by beta-lipotropin. while the alpha-subunit of lutropin had nearly equal the methyl accepting activity of lutropin, the beta-subunit was devoid of accepting activity. the maximum amount of esterification occurred bet ... | 1979 | 218889 |
| replication of herpes simplex virus in human b lymphocytes stimulated by epstein-barr virus. | human b lymphocytes prepared from adult blood or cord blood by the use of neuraminidase-treated sheep red blood cells which did not respond mitogenically to phytohemagglutinin or pokeweed mitogen, responded well to the b95-8 strain of epstein-barr virus, but not to the p3hr-1 strain, with increased incorporation of tritiated thymidine. replication of herpes simplex virus could be demonstrated in cultures of b lymphocytes preincubated with the b95-8 strain. | 1979 | 218893 |
| bent-limb syndrome in lambs raised in total confinement. | a bent-limb syndrome in lambs raised in total confinement was characterized by curvature of the forelimbs. radiographic findings included flaring of the affected long bone and thinning of the growth plate. the main histologic change was endochondral dysplasia of the long bone. in feed samples, all trace minerals analyzed were within recommended concentrations except iron, which was much higher (400 ppm dry matter) than the normal requirement of lambs (70 ppm). all mineral concentrations in serum ... | 1978 | 218916 |
| serologic survey of prevalence of ovine progressive pneumonia in idaho range sheep. | blood samples from 2,310 mature sheep in 3 idaho range flocks were examined by agar gel immunodiffusion to determine the prevalence of ovine progressive pneumonia. the prevalence ranged from 58% for all ages combined in one flock to 90% of cull ewes in another flock. age-specific prevalence rates increased from 16% in yearlings to 83% in ewes greater than or equal to 7 years old. rambouillet sheep had a significantly (p less than 0.01) lower prevalence than sheep of 5 other breeds, whereas one-h ... | 1978 | 218917 |
| diagnostic features of ovine progressive pneumonia. | ovine progressive pneumonia, a chronic, insidious disease of adult sheep, has a relentless course leading to dyspnea, emaciation, and death. clinical observations and serologic tests are adequate for making a tentative diagnosis. the agar gel immunodiffusion test seems to be the best serologic procedure for indicating infection with the virus but cannot be used to predict morphologic changes or clinical disease, inasmuch as many clinically unaffected animals carry the virus. a definitive diagnos ... | 1978 | 218918 |
| in vitro synthesis of a putative precursor to the mitochondrial enzyme, carbamyl phosphate synthetase. | a simple and rapid procedure is described for purification of carbamyl phosphate synthetase from the matrix fraction of rat liver mitochondria. antibodies to the enzyme were raised in sheep and purified from antiserum by affinity chromatography on enzyme-bound sepharose columns. when membrane-free polyribosomes, isolated from a cytosolic fraction of rat liver, were incubated in a messenger-dependent rabbit reticulocyte protein-synthesizing system in the presence of [35s]methionine, the purified ... | 1979 | 218964 |
| complement-fixing immunoglobulin m antibody response in patients with infantile gastroenteritis. | complement-fixing immunoglobulin m antibody to infantile gastroenteritis virus (a rotavirus) was detected with highest sensitivity when the antibody-antigen-complement mixture was incubated at 37 degrees c for 1 h prior to the addition of sensitized sheep erythrocytes. sucrose gradient centrifugation of sera collected sequentially from four patients after infection detected 19s complement-fixing antibody up to 5 weeks, with highest titers at 1 week, after the onset of illness. treatment of the w ... | 1979 | 219023 |
| comparison of the stimulatory effects of ovine, porcine and human follicle-stimulating hormone and of ovine and human luteinizing hormone on the accumulation of cyclic amp by porcine granulosa cells. | the effects of ovine, porcine and human fsh, and ovine and human lh on the accumulation of cyclic amp by porcine granulosa cells obtained from follicles at various stages of maturation were investigated. during incubation periods of 15 min, 10 micrograms ovine fsh pretreated with antiserum to lh or 10 micrograms human fsh resulted in an 11- to 18-fold, five- to ninefold, and less than a twofold increase in intracellular accumulation of cyclic amp by granulosa cells from small (1--2 mm), medium ( ... | 1979 | 219128 |
| precipitation of visna viral proteins by immune sera of rabbits and sheep. | eighteen polypeptides equivalent to 1.2 x 10(6) daltons of visna virus were specifically precipitated by immune sera from rabbits and sheep. the hyperimmunized rabbit antisera contained high concentrations of antibodies against p25 and p14, whereas the sera from sheep actively infected with visna virus showed a large quantity of anti-gp115 antibody. the results indicate that almost all the polypeptides reported previously (f. h. lin, j. virol. 25:207--214, 1978) are virus-specific components of ... | 1979 | 219247 |
| [passive hemagglutination test for individual or epidemiologic diagnosis of human brucellosis]. | coupling of an extract from b. abortus strain 99 to sheep red blood cells by chromium chloride allowed to develop a highly specific and reliable passive hemagglutination test (h). indeed, h was positive in 99.8 per cent sera from 968 known brucella-infected heifers, while tube agglutination revealed only 59 per cent and complement fixation 90.9 per cent positive cases. h was positive in 153 sera from 778 proven or suspected human brucellosis where agglutination and/or complement fixation tests w ... | 1978 | 219486 |
| genetics of resistance of animals to viruses: i. introduction and studies in mice. | | 1978 | 219668 |
| biochemical mechanism of the sodium pump. | | 1979 | 219763 |
| application of antisera to interferon in studying oncogenic viruses. | susceptibility of balb/c mice to infection with moloney sarcoma virus (msv) and to herpes simplex virus type 2 (hsv-2) was considerably increased by administration of sheep anti-mouse interferon (anti-if) serum. the regression of the msv-induced tumors was inhibited when the weanling (three-to-four-week-old) mice were injected with the anti-if serum. using the anti-if serum it has been found that the antagonism between hsv-2 and rauscher leukemia virus in the mouse is not mediated by interferon. ... | 1978 | 219809 |
| specific cleavage of beta-lph and acth by tonin: release of an opiate-like peptide beta-lph (61-78). | | 1979 | 219857 |
| the stem hormone: changes in the adrenocorticotrophin and lipotrophin family tree of different species in foetal and adult life [proceedings]. | | 1979 | 220363 |
| a rapid and specific method for separation of bound and free antigen in radioimmunoassay systems. | this communication reports a method for increasing the speed of separation of bound and free antigen in radioimmunoassay systems with no loss in the specificity of binding. the technique uses a mixture of second antibody and polyethylene glycol. it is not species or antibody specific, and systems using specific first antibodies from rabbit, goat or sheep are all functional. results for the assay of parathyrin, calcitonin and corticotropin are described here, although the system has been shown to ... | 1979 | 220374 |
| 4-aminopyridine and the early outward current of sheep cardiac purkinje fibers. | we have studied the effects of the potassium-blocking agent 4-aminopyridine (4-ap) on the action potential and membrane currents of the sheep cardiac purkinje fiber. 4-ap slowed the rate of phase 1 repolarization and shifted the plateau of the action potential to less negative potentials. in the presence of 4-ap, the substitution of sodium methylsulfate or methanesulfonate for the nacl of tyrode's solution further slowed the rate of phase 1 repolarization, even though chloride replacement has no ... | 1979 | 220376 |
| pathogenesis of visna. iv. spinal fluid studies. | visna is a persistent retrovirus infection of sheep which produces a chronic progressive paralytic disease after an incubation period lasting from months to years. the cerebrospinal fluid (csf) was repeatedly sampled in a group of icelandic sheep which were infected intracerebrally and followed up to 42 months. minimal levels of infectious virus were isolated from the cerebrospinal fluid (csf) up to 4 months after infection after which csf neutralizing antibodies appeared in many sheep. these an ... | 1979 | 220394 |
| multiplication rate of bluetongue virus in the vector culicoides variipennis (diptera: ceratopogonidae) infected orally. | | 1979 | 220423 |
| sera and cerebrospinal fluids from normal uninfected sheep contain a visna virus inhibiting factor. | | 1979 | 220542 |
| large-scale purification of na,k-atpase and its protein subunits from lamb kidney medulla. | procedures are described for the large-scale isolation of purified na,k-atpase (ec 3.6.1.3) from frozen lamb kidney outer medulla and for the separation of its two protein subunits by hydroxyapatite chromatography in sodium dodecyl sulfate (sds). the methods described permit the routine isolation of up to 800 mg of purified na,k-atpase in one week, which can subsequently be separated into 500 mg of mr = 95,000 catalytic subunit and 200 mg of glycoprotein with four sds-hydroxyapatite column runs. | 1979 | 220603 |
| bluetongue virus characterization. | | 1975 | 220625 |
| bluetongue antibody in botswana's domestic and game animals. | bluetongue precipitating antibody was demonstrated in sera of cattle, camels, sheep, goats and seven game species. of the domestic species the percentage of sera positive were; cattle 92%, camels 81%, goats 83% and sheep 36%. sheep sera, unlike those of other domestic species, varied greatly from area to area in the percentage positive. seroconversions were recorded in adult sheep between september and april. in adult cattle there was a gradual decline in the percentage positive with increasing ... | 1979 | 220761 |
| effects of anabolic steroids on liver cell ultrastructure in sheep. | castrated male sheep were sham inplanted or implanted with estradiol, trenbolone acetate or trenbolone acetate in combination with estradiol, and slaughtered after 56 days. ultrastructural and morphometric examination of the livers of steroid-treated sheep showed an increase in the volume density of the cell occupied by rough endoplasmic reticulum and microbodies. in four of the eight sheep treated with trenbolone acetate, either alone or in combination with estradiol, enlarged mitochondria with ... | 1978 | 220776 |
| increased proportion of active sheep erythrocyte rosette-forming lymphocytes and plasma rosette enhancement in sarcoidosis. | we examined blood lymphocyte subpopulations in 20 patients with sarcoidosis, 37 patients with other diseases, and 51 normal subjects. the b-lymphocytes were identified by the presence of surface immunoglobulin or b-lymphocyte-associated antigen. lymphocytes were also centrifuged with sheep erythrocytes for 5 min at room temperature at 200 g, and rosette formation was assayed immediately (active e-rosette-forming t-lymphocytes) or after 60-min incubation at 4 degrees c (total t-lymphocytes). the ... | 1979 | 220896 |
| hormone receptor levels and metabolic activity in the uterus of the ewe: regulation by oestradiol and progesterone. | | 1978 | 220946 |