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peripheral blood lymphocyte response to acute infections in humans.the response of subpopulations of peripheral venous blood lymphocytes to systemic bacterial or viral infections was studied. b-lymphocytes were defined by the presence of surface binding sites for mu chains, which were determined by immunofluorescent staining. t-lymphocytes were defined by the ability to form active sheep cell rosettes. virus-infectible lymphocytes, which may represent activated t-lymphocytes, were defined by the ability to support virus replication. patients with bacterial infe ...1977194835
human rotavirus in lambs: infection and passive protection.a human stool filtrate containing rotavirus which was administered orally to gnotobiotic lambs caused diarrhea, virus excretion, development of antibodies to rotavirus, and pathological changes in the villi of the small intestine. thus, lambs may serve as experimental animals for the study of human rotavirus infections. this model system was used to study passive protection. human immunoglobulin g (igg) containing antibody to rotavirus was fed to lambs 24 to 78 h after birth, and the lambs were ...1977194837
transforming activity and antigenicity of an epstein-barr-like virus from lymphoblastoid cell lines of baboons with lymphoid disease.two lymphoblastoid cell lines were established from baboons with lymphoid disease. cells of these lines were positive for complement and fc receptors but lacked sheep cell receptors, theraby indicating b-cell origin. the cells contained antigens which cross-reacted with epstein-barr virus (ebv), viral capsid antigen (vca), early antigen (ea) and membrane antigen (ma). both lines released virus with in vitro transforming activity for lymphocytes of several primate species including humans. cells ...1977194867
isolation of t-lymphocytes from disaggregated tumors, with high purity and good percentage recovery.a combination of two cell separation methods was utilized for the isolation of thymus-derived lymphocytes (tl) from enzymatically disaggregated tumors. passage through sephadex g-10-glass bead columns to remove adherent cell types followed by exposure to igg-coated sheep red blood cell monolayers for removal of fc receptor-bearing inflammatory cells provided functional tl suspensions of high purity with good percentage recovery.1977195071
characterization of visna virus envelope neuraminic acid.visna virus particles inhibit influenza virus hemagglutination in an assay for neuraminic acid-containing viruses. pretreatment of visna virus with neuraminidase abolished hemagglutination inhibition activity but did not significantly affect attachment, infectivity, or virus-induced cell fusion in sheep choroid plexus cell monolayers.1977195094
the effect of aging on prolactin secretion in rat pituitary monolayer culture. 1977195172
difference in genesis between antigen-induced igm antibody-forming cells and b-cell mitogen-induced igm antibody-forming cells in mouse spleen cell cultures. 1977195184
antigenic shift of visna virus in persistently infected sheep.visna viruses isolated from persistently infected sheep were antigenically distinct from the plaque-purified virus used for inoculation. the selection of antigenic variants under antibody pressure, thought to occur in vivo, was reproduced in sheep cell cultures inoculated with plaque-purified visna virus and maintained in antibody. antigenic shift may be a mechanism for persistence of virus in slow or recurrent viral infections.1977195339
immunoglobulin response to bluetongue virus soluble antigen in subcutaneous chambers.group-specific antibodies were produced by inoculation of bluetongue virus soluble antigen into polyethylene chambers implanted subcutaneously in 8 rabbits and 2 sheep. for comparison, 5 rabbits and 1 sheep were inoculated intramuscularly with the soluble antigen in freund's complete adjuvant. antibodies present in the serum and chamber fluids were detected by the agar gel precipitin or serum-neutralization tests, qualitatively examined by immunoelectrophoresis and immunofluorescence, and quanti ...1977195497
purification and characterization of high-molecular-weight forms of adrenocorticotropic hormone of ovine pituitary glands.a highly purified preparation of high-molecular-weight adrenocorticotropic hormone (acth) was prepared from ovine pituitary glands by dilute acetic acid extraction, oxycellulose fractionation. sephadex gel filtration, and affinity chromatography on immobilized alphap(1-39)acth antibodies. two acth peptides of molecular weights of 24 000 and 34 000 were detected by sodium dodecyl sulfate-acrylamide gel electrophoresis in this preparation. it appeared that the immobilized antibodies adsorbed two f ...1977195594
tumor-associated antigen in bovine and ovine lymphosarcoma.specific tumor-associated antigens were found on the membrane and in the cytoplasm of lymph node cells and peripheral blood lymphocytes (pbl) from cattle and sheep with lymphosarcoma by immunofluorescence tests. materials from 15 cattle with the adult form of lymphosarcoma were examined. cytoplasmic antigen was detected in fixed tumor cells from all 15 cases and in pbl from 9 cases tested. membrane antigen was detected in living cells from 10 of the cases tested. in 3 calf-type cases, cytoplasmi ...1977195721
plasma testosterone levels in male turtles, chrysemys picta, following single injections of mammalian, avian and teleostean gonadotropins. 1977195862
quantitation of antibody to non-hemagglutinating viruses by single radial hemolysis: serological test for human coronaviruses.a single radial hemolysis test was developed for quantitation of specific antibody to non-hemagglutinating viruses. with the human coronaviruses as models, this test utilizes the binding properties of the chromic cation to attach viruses to glutaraldehyde-treated sheep erythrocytes. the most satisfactory system consisted of stabilizing washed sheep erythrocytes with 0.0073% glutaraldehyde for 15 min at 23 degrees c, binding a high concentration of virus to a 25% erythrocyte suspension with 0.001 ...1977195976
pathology of ovine adenovirus type 4 infection in spf lambs: pulmonary and hepatic lesions.seven lambs were challenged with an aerosol of oa4 virus. four developed mild pulmonary oedema and a sparse accumulation of mononuclear cells around blood vessels similar to results reported previously. in addition, three had focal hepatic necrosis and one of these three had a non-suppurative occlusive cholangitis and portal tract lymphangitis and thrombosis. basophilic intranuclear ib's were identified in many necrotic hepatocytes, in lymphatic endothelial cells involved in thrombi but in only ...1977196063
immunodiffusion test for ovine progressive pneumonia.an agar gel immunodiffusion test was developed to detect precipitating antibody against ovine progressive pneumonia (opp) virus. the test was conducted in plastic petri dishes containing 6 ml of 1% purified agar in tris buffer and 8% sodium chloride. wells for serum and antigen were 8 mm in diameter and were cut in a hexagonal pattern 3 cm from a central well. tests were read at 24 and 48 hours. soluble antigen for the test consisted of concentrated nutrient medium removed every 2 weeks from a c ...1977196531
changes in small blood vessels during cyclical luteal regression in sheep. 1977196693
[sodium diethyldithiocarbamate is a stimulating agent of immunity].a single dose of dtc was administered, in a dose-range from 0.6 mg/kg to 25 mg/kg, to mice immunized with 10(8) sheep red cells (src). all doses strongly enhanced plaque-forming spleen cell (pfc) responses, when given either 18 h before, simultaneously to, 6 h or 24 h after src immunization. however, the higher levels of immunostimulation were attained by dtc doses above 5 mg/kg. dtc-induced immunopotentiation was not accompanied by untoward effects, such as acute toxicity, splenomegalia or modi ...1977196717
the effect of the antiherpesvirus drug 5-methoxymethyl-2'-deoxyuridine on the humoral immune response in vivo.5-methoxymethyl-2'-deoxyuridine (mmudr), a drug with potent antiviral activity in vitro against herpes simplex virus, was investigated for its immunosuppressive effects. doses as high as 2000mg/kg given daily for 9 days were not immunosupporessive as judged by the fact that treated animals produced normal immune responses to sheep erythrocytes, brucella bacteria, and herpes simplex virus.1977196730
immunoelectron microscopic localization of herpes simplex virus antigens in rabbit cornea with antihuman igg-antiferritin hybrid antibodies.sheep antihuman igg-antiferritin hybrid antibodies were used for the ultrastructural localization of herpes simplex virus (hsv) antigens in rabbit corneas from animals with herpetic keratitis. in animals with epithelial keratitis in which active viral replication is occurring (6 days after infection), viral antigen was found within nuclei, on nuclear membranes, and on cell surface membranes of epithelial cells. in animals with early necrotizing keratitis in which active viral replication cannot ...1977197043
micro-indirect hemagglutination test for detection of antibody against transmissible gastroenteritis virus of pigs.a micro-indirect hemagglutination (iha) test was developed for detecting antibody against transmissible gastroenteritis (tge) virus of pigs. tge virus propagated in swine kidney cell cultures was highly purified and concentrated by the combination of ammonium sulfate precipitation, treatment with fluorocarbon, and sucrose density gradient centrifugation. tanned sheep erythrocytes were sensitized with purified virus for use in the iha test. the results of testing 104 serum samples collected from ...1977197119
the mitogenic effect of the lymphocytosis promoting factor from bordetella pertussis on human lymphocytes.the purified lymphocytosis promoting factor (lpf) from bordetella pertussis was found to be a potent mitogen for peripheral blood lymphocytes (pbl) from normal adults as well as for cord blood lymphocytes. proliferation occurred in autologous plasma or fetal calf serum, regardless of previous exposure to pertussis infection or immunization. only one adult human serum, from a physician constantly working with b. pertussis, inhibited the mitogenic response to lpf and this serum was shown to contai ...1977197121
high densitiy lipoprotein (hdl) polymorphisms in rabbit: production of antibody to rabbit allotype (r 67) in sheep.antiserum to a rabbit hdl allotype has been produced in a sheep. qualitative and quantitative immunological test of whole rabbit serum and purified hdl polypeptides, as well as association tests, showed that the absorbed sheep immune serum reacts with the hdl allotype referred to as r 67.1977197172
the action of disinfectants on lamb rotavirus. 1977197685
fetal infection with bovine leukemia virus in sheep.several sheep fetuses were thymectomized, and their tails were removed at 58 to 65 days of gestation for tissue culture. bovine leukemia virus (blv) antigens were detected in serial culture of tissues from fetuses whose dams and sires were both blv positive. however, no blv antigens were detected in serial cultures of tissues from fetuses whose dams were negative but whose sire was positive. precolostral serums from 3 of 16 neonatal lambs, whose sire and dams were both blv positive, were blv ant ...1977198128
[changes in the blood cortisol levels during the first week of life in calves (bos taurus) and lambs (ovis aries)].at birth, the plasmatic level of cortisol is very variable and very high in the new-born calf or the newborn lamb. this level diminishes within one day and then varies between 10 and 30 ng/ml during the six following days. in the calf, the secretion of cortisol can be directly stimulated by acth or inhibited at the level of the hypothalamic and hypophyseal system with dexamethasone on the day following birth.1977198151
human lymphocyte subpopulations: suspension-sheep red-blood-cell rosettes. 1977198174
a growth hormone binding factor in the serum of pregnant mice. 1977198197
biological effects of antitestosterone antibodies: relationship between intensity of immunization, antibody titre, and the state of androgen-dependent traits. 1977198255
[testing of the activity of anti-foot-and-mouth disease vaccines]. 1976198292
the effects of hypoxaemia in fetal sheep. 1977198433
infection of british deer and farm animals with epizootic haemorrhagic disease of deer virus. 1977198436
analysis of murine c-type virus structural proteins by rocket and crossed immunoelectrophoresis.we have examined the structural proteins of rauscher murine leukemia virus (r-mulv) by means of rocket immunoelectrophoresis and crossed immunoelectrophoresis, using polyspecific antisera to tween/ether-disrupted purified r-mulv. fifteen different precipitation lines were recognized in virus lysates. using five reference antisera prepared to purified r-mulv-structural proteins, the precipitation lines of p 10, p 15, p 30 and gp 69/70 were identified. these techniques, although less sensitive tha ...1977198476
diagnostic value of igg and igm antibodies to capsid antigen of epstein-barr virus in infectious mononucleosis. 1977198482
induction of simian virus 40 (sv40) transplantation immunity in mice by sv40-transformed cells of various species.specific tumor rejection was obtained with the use of simian virus 40 (sv40)-transformed cells from several species including man, rat, ape, sheep, and hamster. growth of the syngeneic sarcoma mksa in balb/c mice was strikingly inhibited following a single immunization with as few as 10(3) intact, viable cells. non-sv40-transformed cells did not induce tumor rejection activity nor did sv40-transformed lines induce immunity against the 3-methylcholanthrene-induced sarcoma meth a, syngeneic with ...1977198568
visna virus rna synthesis.visna is a classical slow infection in which virus characteristically persists in the face of the host immune response. the agent of this disease belongs to the retravirus group. the persistence of infection and the slow spread of virus are at least in part a consequence of restriction of the expression of virus genetic information in tissues of an infected animal (a. t. haase et al., science 195:175-177, 1977), but the point at which the virus life cycle is interrupted in vivo and the mechanism ...1977198587
direct photoaffinity labeling with cyclic nucleotides. 1977198631
neuromuscular blockade with anti-axoplasmic antibodies.in the process of developing an immunopharmacologic method for identifying a "trophic" protein released from motor nerve terminals, a soluble fraction of peripheral nerve axoplasm was prepared. an attempt was made to eliminate contaminating myelin and basic protein. antibodies were produced to soluble nerve proteins in all sheep immunized. on boosting, after a 6-week interval, the animals became weak, and some could not stand on the fifth day after injection. a distinct component of neuromuscula ...1977198701
["e" rosette formation in "active" t lymphocytes: phenomenon modulated by intracellular level of cyclic amp and gmp (author's transl)].the rosette formation envolving the binding of sheep red blood cells (srbc) with active t lymphocytes was activated when the lymphocytes were incubated with levamisole, acetylcholine or carbamylcholine. similar activation was seen when to the incubation medium was added substances of glucose metabolism (lactate, fumarate or succinate) or triphosphate de adenosina--atp. the lymphocytes incubation with aminophyline, isoproterenol or 2-4-dinitrofenol--dnp--inhibited the rosette formation. the inhib ...1977198854
the airborne dispersal of foot-and-mouth disease virus from vaccinated and recovered pigs, cattle and sheep after exposure to infection.foot-and-mouth disease virus was detected during two periods in the air of looseboxes which housed susceptible, vaccinated or recovered pigs, cattle or sheep exposed to infection. the first was 30 min to 22 h after exposure and occurred in all animals. the second was two to seven days after exposure and occurred with those susceptible and vaccinated animals which developed clinical lesions, and with vaccinated and recovered pigs and sheep, which did not develop clinical lesions. vaccination of a ...1977198862
[quantitative indices and physico-chemical properties of non-specific inhibitors of influenza a virus hemagglutination in the sera of different species of animals and birds].the results of studies of the quantitative values of nonspecific antihemagglutinins of influenza viruses in the sera of birds, laboratory, wild and domestic animals (altogether 27 species) are presented. the antiviral inhibitors characterized by a number of physicochemical properties (sensitivity to heating, kio4, trypsin, rivanol, 2-mercaptoethanol) were divided into 3 groups, sera of sheep, goats and cattle making up a separate group with regard to their sensitivity to heating and treatment wi ...1977198965
adenovirus infection in lambs. ii. experimental infection of lambs.adenovirus strain gy/14 isolated during a natural outbreak was used in experimental infection. three weeks old lambs responded with temperature rise, respiratory symptoms and diarrhoea to the infection. infection spread to a contact animal, too. reisolation of the virus was successful from the nasal discharge and feces from the 3rd to 10th, and the 3rd to 5th day following infection, respectively. in the killed experimental animals the pathological and histological changes observed were similar ...1977199001
experimental infection of calves with an adenovirus isolated from sheep and related to bovine adenovirus type 2 i. clinical and virological studies.colostrum deprived calves were experimentally infected with an adenovirus isolated from sheep and related to bovine adenovirus type 2. the calves showed respiratory symptoms and mild diarrhoea from the third day after infection. laboratory tests revealed the development of leucopenia, lymphopenia, a drop of the ph of the urine and the appearance of pathological changes in the urine. the animals shed the virus in their nasal discharge, faeces and urine. comparing the clinical and virological find ...1977199002
[15 new serotypes of salmonella of subgenus i isolated in africa].filteen new serotypes of salmonella belonging to the sub-genus i have been isolated in africa. isolated in ivory coast: s. dabou (8,20:z4,z23,l,w), s. elokate (9,12:c:1,7), s. assinie (3,10:1,w:z5), s. anna (13,23:z35:e,n,z16), s. mpouto (16:m,t:-), s. banco (28:r:1,7), s. abidjan (38:b:l,w); in senegal: s. bignona (17:b:e,n,z15), s. lode (17:r:1,2), s. derkle (52,e,h:1,7); in tchad: s. moussoro (1,6,14,25:i:e,n,z15), s. drogana (1,4,12,27:r,i:e,n,z15); in algeria: s. hydra (21:c:1,6); in haute- ...1976199365
[ultrastructural aspects of the cell fusion induced by visna virus on sheep choroid plexus cell in culture].sheep choroid plexus cells infected with low multiplicities of infection of visna virus were stellate and had long and thin processes containing filaments and forming cytoplasmic bridges between adjacent cells. enlargement of the bridges resulted in the formation of multinucleated cells. some glycoproteins were clustered on filaments outside the cell. the cytoplasmic changes showed : an intensive protein synthesis; numerous mitochondria closely associated with filaments and some lysosomes and nu ...1977199372
the infection caused by visna virus. a model for the study of slow degenerative diseases of the central nervous system. 1977199878
[additive effect of hyperbaric oxygen and intra-aortal balloon pump in the therapy of experimental coronary disease]. 1977200021
beta-endorphin: a pituitary peptide with potent morphine-like activity. 1977200175
location of antigens associated with bovine leukemia virus.antigens associated with bovine leukemia virus (blv) infection (gp 44/55 and p23) were identified by neutralization and immunofluorescent antibody (fa) tests. only serums containing antibody to gp 45/55 neutralized the virus; antibody to p23 did not. these results indicate that gp 44/55 may be located in the virus envelope. in indirect fa tests, both a cytoplasmic fluorescence and a membrane fluorscence were detected in blv-infected cells. the serum used in the fa test was then absorbed with eit ...1977200198
inhibition of bovine leukemia virus release by antiviral antibodies.peripheral blood lymphocytes from bovine leukemia virus (blv) infected cattle were grown in vitro with serums from blv-infected and uninfected cattle, sheep and rabbits. only serums from infected animals which had antibody to gp 45/55, the major glycoprotein antigen, inhibited the release of virus from the cells. although viral antigens could be detected in the cells themselves, none were detected in the supernatants of cultures grown with these serums. normal serums and serums with antibody onl ...1977200199
dissolution of silicic acid from dusts of kaolin, mica and talc and its relation to their hemolytic activity. -- an in vitro study.hemolysis induced by native kaolin dust was found to run parallel to the amount of silicic acid dissolved from it. native mica and talc dusts were hemolytic only to a small extent and the silicic acid dissolution from these dusts was also smaller in comparison to that of native kaolin. the proportionality between hemolysis and the amount of dissolved silicic acid was not consistently observed in the case of acid, alkali and water treated kaolin, mica and talc dusts.1977200454
mucosal immunity in the respiratory tract of sheep [proceedings]. 1977200505
binding of rat follicle-stimulating hormone to the turtle ovary in vitro and inhibition of the binding by gonadotropin preparations of various vertebrates. 1977200516
effects of chicken and ovine luteinizing hormone on androgen release and cyclic amp production by isolated cells from the quail testis. 1977200522
antibody formation by subcutaneous and omental splenic autotransplants in rats. 1977200644
steroid and prostaglandin concentrations in the plasma of pregnant ewes during infusion of adrenocorticotrophin or dexamethasone to intact or hypophysectomized foetuses.catheters were implanted into 16 ewes and their foetuses between days 110 and 124 of gestation. hypophysectomy was attempted in eight of these foetuses. continuous infusion of synthetic acth (10 microgram/h) or dexamethasone (1mg/24 h) into the foetus, starting between days 124 and 129, induced premature parturition. the concentration of progesterone in the maternal peripheral plasma decreased before parturition in all animals while the level of oestradiol increased in ewes with intact foetuses ...1977200696
expression of the bovine leukemia virus and its internal antigen in blood lymphocytes. 1977200953
[activity of anatoxins against infectious enterotoxemia of sheep, prepared with different adjuvants]. 1977201079
[prevention of anaerobic gastrotoxemia of lambs]. 1977201080
the attachment of encephalomyocarditis virus to erythrocytes from several animal species. 1977201092
vaccination of cattle and sheep with a combined clostridium perfringens types c and d toxoid.cattle and sheep (30 each) were vaccinated with a combined clostridium perfringens type c and c perfringens type d toxoid. vaccination and blood sample collections were made every 2 weeks over a period of 8 weeks. increases in antitoxin titers occurred after the 2nd administration of the 2.0-ml combined product. highest titers were recorded when the 2nd vaccinal dose was given 2 weeks after the first. this confirms reports that response to clostridial antigens is greater when the 2nd immunizing ...1977201186
bluetongue in cattle: effects of culicoides variipennis-transmitted bluetongue virus on pregnant heifers and their calves. 1977201192
bluetongue in cattle: effects of vector-transmitted bluetongue virus on calves previously infected in utero.three of 7 principal calves, after a challenge of immunity exposure by bites of bluetongue (bt) virus-infected culicoides variipennis, became latently infected with bt virus. these calves were born to heifers infected with the homologous virus by bites of c variipennis at 60 or 120 days' gestation. latent bt virus infection was detected by isolation of bt virus from washed erythrocyte samples obtained from the calves at 57, 100 to 102, 200 to 202, 300 to 302, and 400 to 402 days after challenge ...1977201193
bluetongue in cattle: repeated exposure of two immunologically tolerant calves to bluetongue virus by vector bites.a heifer and steer that were immunologically tolerant to bluetongue (bt) virus became immunologically competent after repeated exposures by bites of bt virus-infected culicoides variipennis. immunologic tolerance ended in the heifer at 25 months of age, after the 2nd exposure to the virus, and in the steer at 22 months, after the 4th exposure. high hemic concentrations of bt virus were detected in both animals after they became immunologically competent, but neither developed an overt bt clinica ...1977201194
plant particles in kidneys and mesenteric lymph nodes of sheep. 1977201236
effects of peptides of pituitary origin on the formation of c21 steroid by fetal calf adrenal cells in culture. 1977201254
slow viral diseases of the central nervous system: transmissibility vs. communicability. 1977201420
studies on the occurrence and distribution of hi antibodies against some arboviruses in the serum of domestic mammals in puglia.the virological and serological studies previously carried out on arboviruses in italy are reviewed. the presence of antibodies to 11 arboviruses was investigated in the serum of various domestic animals (100 horses, 107 pigs, 102 sheep, 205 goats, 100 cattle and 200 dogs) from some areas of puglia. the techniques are described. the results, given in tables and discussed in detail, support the hypothesis that in this region also there are arboviruses circulating, particularly those of group b.1977201546
effects of low-chloride solutions on action potentials of sheep cardiac purkinje fibers.the rapid repolarization during phase 1 of the action potential of sheep cardiac purkinje fibers has been attributed to a time- and voltage-dependent chloride current. in part, this conclusion was based on experiments that showed a substantial slowing of phase 1 when larger, presumably impermeant, anions were substituted for chloride in tyrode's solution. we have re- examined the electrical effects of low-chloride solutions. we recorded action potentials of sheep cardiac purkinje fibers in norma ...1977201723
experimental infection of nigerian sheep and goats with bovine virus diarrhoea virus.apart from leucopenia and a low grade pyrexia nigerian goats showed no ill effects following inoculation with bovine virus diarrhoea virus. sheep showed a variable leucopenia without pyrexia. no difficulty should arise in differentiating bvd infections from peste des petits ruminants.1977202049
induction of premature parturition in sheep: adrenocorticotrophin and corticosteroid changes during infusion of synacthen into the foetus.synacthen (10 microgram/h) infused into foetal sheep at about day 125 of gestation caused premature delivery within 4 days. during the first 24 h of the infusion the secretion of adrenocorticotrophin was depressed and the assayable hormone concentration was within 500-1000 pg/ml. subsequently, large fluctuations occurred in the adrenocorticotrophin concentration in the plasma of foetal sheep which were consistent with the stimulation of adrenocorticotrophin secretion. no transfer of synachthen t ...1978202133
adrenocorticotrophin and corticosteroid changes during dexamethasone infusion to intact and synacthen infusion to hypophysectomized foetuses.synacthen has been infused at 10 microgram/h into 4 hypophysectomized foetal sheep. this caused a rise in adrenocorticotrophin to a mean value of 512 pg/ml which remained fairly constant and did not show the large fluctuations seen on infusion into intact foetuses. the synacthen half-life in the circulation had a mean value of 0.27 min. hypophysectomy did not necessarily delay the foetal corticosteroid response to synacthen infusion. the corticosteroid concentration achieved had a mean value of ...1978202134
prevalence of ovine progressive pneumonia in a sampling of cull sheep from western and midwestern united states.ovine progressive pneumonia was found to be prevalent in many major sheep-producing areas in the united states. the incidence was determined in a sampling of cull sheep (5 to 10 years of age) at slaughter. diagnostic criteria were based on finding viral-specific immunoglobulins, virus, or lesions. each method indicated a high incidence in samplings from midwestern and northwestern states and a relatively low incidence in the sampling from southwestern states. of those sheep examined, precipitati ...1977202181
rotavirus infection in lambs: pathogenesis and pathology.experimental lamb rotavirus infections were studied by immunofluorescence, histopathology and electron microscopy of tissues from infected gnotobiotic lambs killed at intervals from the incubation period to recovery. the rotavirus was demonstrated by immunofluorescence only in epithelial cells of villi in the small and large intestine, and virus antigen was most abundant during the incubation period. an increased enterocyte turnover rate was suggested by the rapid movement of virus-infected cell ...1977202226
the amino acid sequence of cytochrome c from the blowfly lucilia cuprina.the amino acid sequence of cytochrome c isolated from the sheep blowfly lucilia cuprina has been determined by comparison of the compositions of the tryptic peptides to those predicted from the published sequences of cytochromes c from other insects. cytochrome c from l. cuprina differs at a single residue when compared to cytochrome c from the screw worm fly haematobia irritans, a species belonging to the same order as the blowfly. this substitution, proline for alanine, has been located at pos ...1978202328
developmental aspects of the pituitary-adrenal axis response to hemorrhagic stress in lamb fetuses in utero.plasma acth and corticosteroid concentrations were measured by radioimmunoassay in chronically catheterized fetuses of 32 pregnant sheep. fetal plasma acth levels 38+/-5 pg/ml (means+/-sem) were slightly (p < 0.05) lower than maternal 54+/-4 pg/ml levels. no general rise in fetal plasma acth concentration was noted before 140 days gestation; however, fetal plasma corticoid levels began to increase after about 125 days. this suggested that an increase in fetal adrenal responsiveness to endogenous ...1978202613
[role of fetal hormones in the growth of the fetus]. 1977202703
development of fetal pituitary-adrenal function. 1977202804
duodenal glucose infusion and hepatic enzyme activities in sheep. 1977202965
current knowledge of multiple sclerosis. 1978203047
[c-cells and c-cell tumors (author's transl)]. 1977203138
[experimental infection of man using viral strains of bovine papular stomatitis, orf, pseudocowpox and milker's nodule].an account is given of the close correlations that exist between virus strains of bovine papular stomatitis, orf, pseudocowpox, and milker's nodule. reference is made to literature data on natural infection of man with the above virus strains. a report then is presented on experimental infection of human volunteers, using paravaccine birus. while fairly tough and elevated nodules, 4 mm to 5 mm in diameter, were produced on the probends' skin, no re-isolation of virus was achieved.1977203242
mitogenic andadjuvant activities of polysaccharides from the cellular slime mold, dictyostelium discoideum nc-4.cell surface and extracellular polysaccharide fractions obtained from dictyostelium discoideum nc-4 cultured in bacteria-free medium showed strong b-cell mitogenic activities. upon periodate treatment of the extra-cellular polysaccharide fraction this activity completely disappeared. the extracellular polysaccharide fraction could also enhance the antibody response in vitro against sheep red blood cells.1978203332
immunosuppressive effects of lymphocyte (type ii) and leucocyte (type i) interferon on primary antibody responses in vivo and in vitro.we have tested the hypothesis that type ii interferon (if), released by immune lymphocytes after in vivo stimulation with tuberculin, has immunosuppressive effects. mycobacterium bovis (bcg) infected mice injected with tuberculin showed a very intense suppression of antibody response to sheep erythrocytes. sera containing lymphocyte if strongly inhibited primary immune responses to sheep erythrocytes in cultures. addition of macrophages could not counteract the in vitro immunosuppressive effects ...1977203424
two e-rosette-forming lymphoid cell lines.two e-rosette (spontaneous rosette with sheep red blood cells)-forming lymphoid cell lines were established. one (hpb-all) was derived from a young male japanese patient with acute lymphoblastic leukemia (all), and the other (hpb-mlt) was from a 62-year-old female japanese patient with a leukemic t-lymphoid malignancy. formation of e rosettes, absence of any immunoglobulin determinants, absence of ebna (epstein-barr virus associated nuclear antigen) and very limited stimulating ability in mixed ...1978203546
specific lethality of silica for human peripheral blood mononuclear phagocytes, in vitro.human peripheral blood mononuclear cells, cultured in the presence of 100 microgram/ml protein-coated silica particles, were studied to determine changes in number and function of monocytes, immunoglobulin bearing (b), sheep red blood cell rosetting (t) lymphocytes and the effector cells of antibody dependent cell-mediated cytotoxicity (adcc). after 24-48 h, phagocytic cells were effectively eliminated from culture but there was no significant reduction in number or function of t or b lymphocyte ...1978203631
effect of prostaglandin e2 on the concentration of cortisol in the plasma on newborn lambs. 1978203642
effect of infectious bursal disease virus on the immunological responsiveness of the chicken.the immunological response of chickens infected at 28 days of age with infectious bursal disease virus (ibdv) was examined. cellular immunity was studied by the graft-versus-host reaction and delayed type hypersensitivity skin reactions to tuberculin. antibody responses to sheep red blood cells (s-rbc's) were tested by a direct hemagglutination test. circulating levels of immunoglobulins (ig's) igm, igg and iga were monitored by the radial immunodiffusion test. the results of the graft-versus-ho ...1977203923
lack of maedi viral related rna in pulmonary carcinoma of sheep (jaagsiekte).molecular hybridisation with radioactively labelled dna complementary to the rna of the maedi virus was used to probe for homologous rna in the polysome fraction of pulmonary carcinomas (jaagiekte) of awassi sheep. no sequence homology was detected, which suggests that maedi (or visna) virus is not implicated in the aetiology of pulmonary carcinoma of sheep.1978203989
serological studies of mucosal disease virus in england and wales.a survey of the cattle population in england and wales showed that about 62 per cent of animals had neutralising (sn) antibody to bvd-md virus in their serum. fewer seropositive individuals were detected by immunodiffusion (53 per cent), and complement fixation (cf) tests (34 per cent). the prevalence of antibody was not affected by herd size, but some variation was found between various regions of the country, and the proportion of animals seropositive rose markedly with age. the immunodiffusio ...1978203990
lymphocyte function of michigan dairy farmers exposed to polybrominated biphenyls.michigan dairy farm residents ate farm products containing polybrominated biphenyls (pbb's) after the accidential contamination of animal feed with the chemical in that state in 1973. the circulating blood lymphocytes of these residents show significant changes. abnormalities include decreases in the numbers and percentages of peripheral blood lymphocytes that form rosettes with either sheep erythrocytes alone or with sheep erythrocytes sensitized with antibody and complement, increases in lymph ...1978204005
[detection of the parainfluenza virus in respiratory pathology in lambs]. 1978204101
[principles of formation in viral infections of immune complexes, their persisting fraction and its neutralization with antiglobulin, complement and homoreactant]. 1978204134
bronchiolo-alveolar cell carcinoma. animal model: pulmonary adenomatosis of sheep, pulmonary carcinoma of sheep, pulmonary carcinoma of sheep (jaagsiekte). 1978204194
heterogeneity of culicoides variipennis field populations to oral infection with bluetongue virus.a standard colonized population (000 line) of culicoides variipennis (coquillett) was used as the control for comparing the infection rate (ir) responses of different field populations of the vector to oral infection with several strains of bluetongue virus (btv) that belonged to four serotypes. three field populations from colorado and oregon tested concurrently in 1969 were differently susceptible to three btv strains representing three serotypes. the ir's of each of the three populations vari ...1978204209
orungo virus of the orbivirus taxon. experimental sheep infection and neutralizing antibodies in domestic and wild animals in nigeria. 1978204395
enzyme immunoassay for human apolipoprotein b, the major protein moiety in low-density- and very-low-density lipoproteins.we used enzyme immunoassay to measure apolipoprotein b concentration in human plasma. pure lipoprotein b was isolated from serum samples of fasting normolipidemic subjects by sequential preparative ultracentrifugation and coated to a polystyrene tube surface by adsorption. human serum samples and rabbit antiserum to human apolipoprotein b were incubated with the solid-phase lipoprotein b. soluble antigen competed with solid-phase antigen for binding to antibodies. after washing, peroxidase-label ...1978204430
[in-flow of contrast into the liver--a rare complication during lymphography (author's transl)].a lymphogram is described in which lipiodol was found to have reached the liver from pelvic lymphatics. the common iliac and para-aortic lymph nodes had been invaded by tumour from a squamous carcinoma and this had produced complete lymphatic obstruction of the normal lymph paths. in addition, there was occlusion of the bifurcation of the iliac veins due to tumour, a finding also described by other authors. the causes for the finding of lipiodol in the liver are discussed. the pathway by which t ...1978204555
observations on crystals found in the intestinal cells of haemonchus contortus and in the intestinal lumen of ostertagia ostertagi. 1978204587
bluetongue studies with sentinel cattle in kenya.bluetongue antibody of 19 different serological types was found in a group of sentinel cattle near nairobi. a continuous challenge by these 19 strains occurred each year in this location. the sero-conversion rates were shown to vary from year to year and rainfall had no obvious effect upon the conversion rates. the 19 different strains of virus were also shown to be active at five widely scattered sites in different parts of the country.1978204705
variation of properties of chrysotile asbestos subjected to milling.mechanical milling is commonly used to produce short chrysotile asbestos for experimental purposes. such manipulation also decreases fiber crystallinity, alters si-o and mg-o interlayer bonding, induces coordination changes in the brucite layer, diminishes the ability of fiber to reduce specific free radicals and physisorb organic molecules, and decreases hemolytic potency and antagonist sorption capabilities. the degree of alteration is related to the time of milling. results of biological expe ...1978204800
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