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reservoirs of staphylococcus aureus in meat sheep and dairy cattle.the objective of the study was to investigate reservoirs and transmission of s. aureus in ewes and lambs in 3 meat sheep flocks. repeated sampling of milk, teat skin, nasal- and vaginal mucous membranes was performed and samples were analysed for s. aureus. for comparison, samples were also collected from cows and young heifers in 3 dairy cattle herds. selected isolates were compared by pulsed-field gel electrophoresis (pfge). s. aureus was detected in 8 (1.5%) of 520 milk samples from ewes and ...201121903348
hair loss in women: medical and cosmetic approaches to increase scalp hair fullness.androgenetic alopecia affects both men and women. in men it produces male pattern hair loss with bitemporal recession and vertex baldness. in women it produces female pattern hair loss (fphl) with diffuse alopecia over the mid-frontal scalp. fphl occurs as a result of nonuniform hair follicle miniaturization within follicular units. diffuse alopecia is produced by a reduction in the number of terminal fibres per follicular unit. baldness occurs only when all hairs within the follicular units are ...201122171680
experimental interspecies transmission studies of the transmissible spongiform encephalopathies to cattle: comparison to bovine spongiform encephalopathy in cattle.prion diseases or transmissible spongiform encephalopathies (tses) of animals include scrapie of sheep and goats; transmissible mink encephalopathy (tme); chronic wasting disease (cwd) of deer, elk and moose; and bovine spongiform encephalopathy (bse) of cattle. the emergence of bse and its spread to human beings in the form of variant creutzfeldt-jakob disease (vcjd) resulted in interest in susceptibility of cattle to cwd, tme and scrapie. experimental cross-species transmission of tse agents p ...201121908269
Investigation of an Escherichia coli O145 outbreak in a child day-care centre--extensive sampling and characterization of eae- and stx1-positive E. coli yields epidemiological and socioeconomic insight.On October 29th 2009 the health authorities in the city of Trondheim, Norway were alerted about a case of Shiga toxin-positive E. coli (STEC) O145 in a child with bloody diarrhoea attending a day-care centre. Symptomatic children in this day-care centre were sampled, thereby identifying three more cases. This initiated an outbreak investigation.201121902833
the optional venatech(™) convertible (™) vena cava filter: experimental study in sheep.purpose: retrieval of optional caval filters may be impaired by filter tilting, migration, fracture, or embedding in the ivc wall. the goal of this experimental study was to evaluate a new optional filter, convertible by unlocking and removing the filter head. methods: forty-nine pre-alp sheep (average weight, 55 kg) were anesthetized. ivc was catheterized via the right femoral vein (n = 46) or via the internal jugular vein (n = 3) with a 12.9-f sheath. venatech(™) convertible(™) ivc filters wer ...201121927988
Identification of two new serotypes within serogroup B of Dichelobacter nodosus.The present study records the strain-specific molecular typing system for Dichelobacter nodosus (D. nodosus) based on genetic analysis of fimA locus. Based on the study two new serotypes B5 and B6 are reported within the serogroup B. Out of 200 swab samples collected randomly from foot lesions of footrot affected sheep from all the districts of Kashmir, India, 122 (61.0%) detected positive for D. nodosus. Serogroup B was predominantly prevalent in 83.60% of positive samples. Restriction fragment ...201122197966
heterogeneity of variance components for preweaning growth in romane sheep due to the number of lambs reared.the pre-weaning growth rate of lambs, an important component of meat market production, is affected by maternal and direct genetic effects. the french genetic evaluation model takes into account the number of lambs suckled by applying a multiplicative factor (1 for a lamb reared as a single, 0.7 for twin-reared lambs) to the maternal genetic effect, in addition to including the birth*rearing type combination as a fixed effect, which acts on the mean. however, little evidence has been provided to ...201121896209
Modification of milk fatty acid composition by feeding forages and agro-industrial byproducts from dry areas to Awassi sheep.The study tested the hypothesis that certain underused forages and agro-industrial byproducts available in dry areas may positively influence fatty acid (FA) composition and antioxidative properties of milk by their contents of residual oil or phenolic compounds or both. Sixty multiparous fat-tailed Awassi ewes were allocated to 6 groups in a completely randomized block design. During 50 d, the ewes were group-fed 2.5 kg of dry matter/d per ewe 1 of 6 isonitrogenous and isoenergetic diets (forag ...201121854938
Antibody repertoire development in fetal and neonatal piglets. XX. B cell lymphogenesis is absent in the ileal Peyer's patches, their repertoire development is antigen dependent, and they are not required for B cell maintenance.The continuous ileal Peyer's patches (IPP) of sheep are regarded as a type of mammalian bursal equivalent where B cells diversify their repertoire in an Ag-independent fashion. Anatomically and developmentally similar IPP occur in swine. Resection of ~90% of the IPP in piglets at birth did not alter Ig levels in serum and secretions or retard diversification of the Ab repertoire when animals were maintained in isolators and colonized with a defined gut flora. Resection or sham surgery elevated I ...201122013126
A Clinical Case of Severe Anemia in a Sheep Coinfected with Mycoplasma ovis and 'Candidatus Mycoplasma haemovis' in Hokkaido, Japan.A 2-year-old East Friesian sheep imported from Australia exhibited severe anemia after contagious pustular dermatitis in Hokkaido, Japan. Hemoplasma infection was confirmed in blood smears. Both Mycoplasma ovis and 'Candidatus Mycoplasma haemovis' were detected by PCR and sequence analyses. In the epidemiological analysis, dual pathogens were detected in 6 of 12 (50.0%) sheep imported from Australia with the infected ewe at the same time, 1 of 5 (20.0%) sheep introduced from a domestic farm in H ...201121869571
Prenatal repair of myelomeningocele with aligned nanofibrous scaffolds-a pilot study in sheep.Spinal cord damage in myelomeningocele (MMC) results from abnormal cord development and subsequent local trauma. Prenatal surgery prevents additional neural injury. However, existing damage is not reversed. Biodegradable nanofibrous scaffolds (NSs) promote regeneration of neural tissues. They mimic the microtopography of the extracellular matrix and guide tissue formation and organization. The purpose of this pilot study was to evaluate the practicality and safety of using biodegradable NS as a ...201122152865
???????Bactericidal effect of ultraviolet C (UVC), direct and filtered through transparent plastic, on gram-positive cocci: an in vitro study.The prevalence of wound infections caused by multidrug-resistant (MDR) bacteria is increasing along with concern about widespread use of antibiotics. In vitro studies have shown that ultraviolet radiation, especially UVC, is both an effective bactericidal and antifungal. However, evidence about its bactericidal effect on wounds covered with transparent dressings remains inconclusive. Transparent dressings are used to retain moisture over the wound as part of an intermittent negative pressure dre ...201121904015
Stem cell niche and postnatal muscle growth.Stem cell niche plays a critical role in regulating the behavior and function of adult stem cells that underlie tissue growth, maintenance and regeneration. In the skeletal muscle, stem cells, called satellite cells, contribute to postnatal muscle growth and hypertrophy, and thus, meat production in agricultural animals. Satellite cells are located adjacent to mature muscle fibers underneath a sheath of basal lamina. Microenvironmental signals from extracellular matrix mediated by the basal lami ...201122100594
analysis and comparison of proteomic profiles of tear fluid from human, cow, sheep, and camel eyes.to investigate the tear proteome profiles of human, cow, sheep, and camel comparatively and to explore the difference of tear protein profiles among different species.201122025569
Canine periodontitis: The dog as an important model for periodontal studies.Periodontal disease (PD) refers to a group of inflammatory diseases caused by bacterial plaque in the periodontium and ranges from an early stage (gingivitis) to an advanced stage (periodontitis). It is a multifactorial disease that results from the interaction of the host defence mechanisms with the plaque microorganisms. Early detection, diagnosis and treatment are essential in the control of this disease. PD has an enormous impact on human and veterinary medicine due to its high prevalence. T ...201121940182
ToF-SIMS analysis of dexamethasone distribution in the isolated perfused eye.To illustrate the ability of time-of-flight secondary ion mass spectrometry (ToF-SIMS) to characterize and demonstrate the spatial distribution of dexamethasone within ocular tissues.201121948549
laparoscopic ovum collection in sheep: gross and microscopic evaluation of the ovary and influence on ooctye production.gross and microscopic evaluations of the ovaries of sheep subjected to successive ovum collection were performed with the objective of identifying if there was interference in oocyte production and morphology of gonads. gross evaluation of the internal genital tract was also performed. eighteen ewes of the santa inês breed were randomly distributed into three experimental groups of six animals each; g0, g1 and g9 with no, one and nine repetitions, respectively. estrous synchronization was achiev ...201121907507
Proteinase K-resistant material in ARR/VRQ sheep brain affected with classical scrapie is composed mainly of VRQ prion protein.Classical scrapie is a prion disease in sheep and goats. In sheep, susceptibility to disease is genetically influenced by single amino acid substitutions. Genetic breeding programs aimed at enrichment of arginine-171 (171R) prion protein (PrP), the so-called ARR allele, in the sheep population have been demonstrated to be effective in reducing the occurrence of classical scrapie in the field. Understanding the molecular basis for this reduced prevalence would serve the assessment of ARR adaptati ...201121917981
Development of a panel of highly sensitive, equivalent assays for detection of antibody responses to velaglucerase alfa or imiglucerase enzyme replacement therapy in patients with Gaucher disease.Anti-drug antibodies are elicited by virtually all therapeutic proteins, and standardized assays are required for clinical monitoring of patients as well as for comparing antibody response to different therapeutic proteins in clinical trials. Velaglucerase alfa and imiglucerase are enzyme replacement therapies for the long-term treatment of type 1 Gaucher disease, a lysosomal storage disease resulting from an inherited deficiency of the enzyme glucocerebrosidase. We used state-of-the-art tools t ...201121846471
Insecticidal and repellent effects of tea tree (Melaleuca alternifolia) oil against Lucilia cuprina.Laboratory studies were conducted to assess the effect of tea tree oil (TTO) from Melaleuca alternifolia (terpinen-4-ol chemotype) against different stages of the Australian sheep blowfly Lucilia cuprina. When applied to wool, 3% TTO formulation repelled gravid female L. cuprina and prevented oviposition for six weeks. Formulations containing 1% TTO caused 100% mortality of L. cuprina eggs and 1st instar larvae and 2.5% TTO caused mortality of most second and third instar larvae in agar feeding ...201121899953
Neurochemical Properties of Aquaporin 1-Expressing Sensory Neurons from the Ovine Trigeminal Ganglion.Aims of the present study were to investigate the distribution and morphology of aquaporin 1-immunoreactive (AQP1-IR) neurons in the sensory ganglia of the sheep. Double immunohistochemical staining was applied to figure out whether substance P (SP), calcitonin gene-related peptide (CGRP) and galanin are present in AQP1-bearing primary afferent neurons. The expression of AQP1 was present only in trigeminal ganglion, whereas in nodose ganglion, jugular ganglion as well as C(1) -C(7) dorsal root ...201122150518
The osteoinductivity of silicate-substituted calcium phosphate.The osteoinductivity of silicate-substituted calcium phosphate and stoichiometric calcium phosphate was investigated with use of ectopic implantation. Implants with a macroporosity of 80% and a strut porosity of 30% were inserted into sites located in the left and right paraspinal muscles of six female sheep.201122159858
myocardial performance and its acute response to angiotensin ii infusion in fetal sheep adapted to chronic anemia.fetal chronic anemia causes lengthening of cardiomyocytes. in adults, severe left ventricular overload may lead to irreversible ventricular dysfunction. we hypothesized that in sheep fetuses with chronic anemia, remodeled myocardium would less successfully respond to angiotensin ii (at ii) infusion than in fetuses without anemia. a total of 14 ewes with twin pregnancy underwent surgery at 113 ± 1 days of gestation. after a recovery period, anemia was induced by isovolumic hemorrhage in 1 fetus o ...201122051849
evaluation of antioxidant status and oxidative stress in sheep naturally infected with babesia ovis.the present study aimed to assess antioxidant status and oxidative stress in sheep naturally infected with babesia ovis. red blood cell (rbc) count, hemoglobin (hb) concentration, packed cell volume (pcv), piroplasm parasitemia percentage, malondialdehyde (mda) concentration, erythrocyte superoxide dismutase (sod), glutathione peroxidase (gsh-px), catalase (cat) activities and total antioxidant capacity (tac) were determined in 52 sheep naturally infected with b. ovis as well as same number of h ...201122030375
inflammation in utero exacerbates ventilation-induced brain injury in preterm lambs.background: cerebral blood flow disturbance is a major contributor to brain injury in the preterm infant. the initiation of ventilation may be a critical time for cerebral haemodynamic disturbance leading to brain injury in preterm infants, particularly if they are exposed to inflammation in utero. we aimed to determine whether exposure to inflammation in utero alters cardiopulmonary haemodynamics resulting in cerebral hemodynamic disturbance and related brain injury during the initiation of ven ...201122052871
Genetic and antigenic characterization of small ruminant lentiviruses circulating in Poland.Small ruminant lentivirus (SRLV) infections are widespread in Poland, but the genetic features of sheep viruses are still lacking and limited to partial gag sequences for goat viruses. In this study, segments from the gag and env genes of Polish SRLV strains screened by heteroduplex mobility assay were subjected to genetic analyses. Subtype A1 was found in both sheep and goats, while subtypes B1 and B2 were found in goats and sheep, respectively. In addition, two novel subtypes (named A12 and A1 ...201122155513
Brucellosis control in Tajikistan using Rev 1 vaccine: change in seroprevalence in small ruminants from 2004 to 2009.Cross-sectional serological surveys of 13,006 small ruminants were conducted in 2003 to establish baseline levels of seropositivity to brucellosis and of 12,102 again in 2009 to evaluate the efficacy of controls based on biannual conjunctival vaccination with Rev 1. Seroprevalence dropped by 80 per cent in five years in eight pilot programme districts where vaccination was generally well implemented, and the prevalence of households with evidence of infection in their animals dropped from 25.1 t ...201122121155
The development of PCR methodology for the identification of species of the tapeworm Moniezia from cattle, goats and sheep in central Vietnam.The aims of this study were to investigate the prevalence of Moniezia spp. in domestic ruminants in central Vietnam and to develop a polymerase chain reaction (PCR) technique to distinguish M. expansa from M. benedeni. Among 2040 examined domestic animals (540 cattle, 800 goats, 700 sheep) Moniezia was recovered from 5.4% of cattle, 16.4% of sheep and 20.6% of goats. A set of primers for PCR was designed to classify M. expansa and M. benedeni based on the amplification of DNA corresponding to th ...201122071022
a possible case of caprine-associated malignant catarrhal fever in a domestic water buffalo (bubalus bubalis) in switzerland.abstract:201122132808
northern ireland disease surveillance, april to june 2011. 201121936069
Seroprevalence of Coxiella burnetii antibodies in sheep and goats in the Republic of Ireland. 201121881024
ammonia emissions during vermicomposting of sheep manure.the effect of c:n ratio, temperature and water content on ammonia volatilization during two-phase composting of sheep manure was evaluated. the aerobic phase was conducted under field conditions. this was followed by phase ii, vermicomposting, conducted in the laboratory under controlled conditions of water content (70% and 80%) and temperature (15 and 22 °c). the addition of extra straw lead to a 10% reduction in nh3 volatilization compared to sheep manure composted without extra straw. tempera ...201121996478
Paratuberculosis control: a review with a focus on vaccination.ABSTRACT: Mycobacterium avium subsp. paratuberculosis (MAP) infection causes in ruminants a regional chronic enteritis that is increasingly being recognized as a significant problem affecting animal health, farming and the food industry due to the high prevalence of the disease and to recent research data strengthening the link between the pathogen and human inflammatory bowel disease (IBD). Control of the infection through hygiene-management measures and test and culling of positive animals has ...201122035107
A suitable duplex PCR for ovine embryo sex and genotype of PrnP gene determination for MOET-based selection programmes.The objective of this study was to test the suitability of a duplex PCR assay for sex and scrapie resistance genotype determination in fresh embryos. Duplex PCR amplified a repetitive and specific fragment of Y chromosome, used for sex diagnosis, and a PrnP fragment. PrnP codons 134 and 156, and codon 171 were genotyped by restriction fragment length polymorphisms and allele-specific PCR, respectively, after re-amplification of PrnP fragment. The specificity of the method was first assessed by t ...201121851426
association between variation in faecal egg count for a mixed field-challenge of nematode parasites and ovine mhc-dqa2 polymorphism.the selection of sheep that are resistant to gastrointestinal parasites and have lower faecal egg counts (fecs) has been the subject of extensive research. this has led to the speculation that the major histocompatibility complex (mhc) genes could be used as markers to reduce fec. in this study, associations between variation in ovine mhc-dqa2 and various measures of fec recorded at two times (approximately 4 and 9 months of age) were investigated in a large group of new zealand lambs (n=4676), ...201121945177
in vivo evaluation of virtual electrode mapping and ablation utilizing a direct endocardial visualization ablation catheter.visualization catheter with virtual electrode ablation. background: radiofrequency (rf) ablation utilizing direct endocardial visualization (dev) requires a "virtual electrode" to deliver rf energy while preserving visualization. this study aimed to: (1) examine the virtual electrode rf ablation efficacy; (2) determine the optimal power and duration settings; and (3) evaluate the utility of virtual electrode unipolar electrograms. methods and results: the dev catheter lesions were compared to le ...201121914025
klebsiella pneumoniae type 3 fimbriae agglutinate yeast in a mannose resistant manner.the ability of bacterial pathogens to express different fimbrial adhesins plays a significant role in virulence. thus, specific detection of fimbrial expression is an important task in virulence characterization and epidemiological studies. most clinical klebsiella pneumoniae isolates express type 1 and type 3 fimbriae characterized by mediating mannose sensitive agglutination of yeast cells, and agglutination of tannic acid treated ox red blood cells (rbcs), respectively. we have observed that ...201122016558
Polymeric cyclosporine-A nanoparticles for ocular application.In the present study, cyclosporine A (CsA) was incorporated successfully into cationic Eudragit RS 100 nanoparticles (EPNs) aiming ocular application. Physicochemical characterization of the EPNs prepared was performed during the storage period of 6 months. Following in vitro release tests, sheep were used in in vivo studies where 100 microL of formulation was applied to both eyes (n = 6) under veterinarian supervision. Aqueous and vitreous humour samples were collected at predetermined time int ...201122195490
an overview of brucellosis.brucellosis is the most important zoonotic disease caused by brucella species comprising gram negative, facultative, intracellular pathogens. the true incidence of human brucellosis is unknown for most countries of the world including bangladesh. but brucellosis is not uncommon in our country. due to its increasing incidence in many countries of the world it is an important issue now days. domestic animals such as cattle, goats, sheep, pigs, camel, buffalo and dogs serve as a reservoir hosts. tr ...201122081201
Effect of reduced ferulate-mediated lignin/arabinoxylan cross-linking in corn silage on feed intake, digestibility, and milk production.Cross-linking of lignin to arabinoxylan by ferulates limits in vitro rumen digestibility of grass cell walls. The effect of ferulate cross-linking on dry matter intake (DMI), milk production, and in vivo digestibility was investigated in ad libitum and restricted-intake digestion trials with lambs, and in a dairy cow performance trial using the low-ferulate sfe corn mutant. Silages of 5 inbred corn lines were fed: W23, 2 W23sfe lines (M04-4 and M04-21), B73, and B73bm3. As expected, the W23sfe s ...201121943763
Effect of field pea (Pisum sativum L.) level on intake, digestion, ruminal fermentation and in vitro gas production in sheep fed maintenance diets.The objective of this study was the characterization of the nutritive value, ruminal fermentation and nutrient digestibility of field pea (Pisum sativum L.) hay (FPH) by-product through in situ, in vitro and in vivo tests. A FPH diet was administered ad libitum to four sheep (live weight (LW) 38.0 ± 2.0 kg) at four inclusion levels (0, 25, 50 and 75%) over a conventional sorghum grain/oat straw ration in a 4 × 4 Latin square design. The in situ degradability of FPH was 67% dry matter (DM), 71% c ...201121951901
prevalence of neospora caninum antibodies in sheep and goats in pakistan.abstract the purpose of the present study was to obtain seroepidemiological information on the neospora caninum infection status of sheep and goats in different areas of punjab province and azad kashmir (pakistan). a cross-sectional study, using a competitive elisa showed an overall 27.7% (35 of 128) (95% ci ? 7.7%) and 8.6% (13 of 142)(95% ci ? 4.6%) seroprevalence of n. caninum antibodies in sheep and goats, respectively. the difference in seroprevalence between sheep and goat populations wa ...201121854220
evaluation of serological tests for detecting tick-borne encephalitis virus (tbev) antibodies in animals.tick-borne encephalitis (tbe) in animals is not well understood yet. tbe virus (tbev) serology in several host species could be valuable for epidemiological analyses in the field as well as for the detection of clinical cases. however, performance and suitability of the available test systems are not well assessed. therefore, we evaluated two commercial tbev-elisa kits in a pilot study and compared them for their suitability in veterinary applications. for this purpose, we tested 163 field colle ...201122191165
A comparative anatomical study of the human knee and six animal species.PURPOSE: Animal models are an indispensable tool for developing and testing new clinical applications regarding the treatment of acute injuries and chronic diseases of the knee joint. Therefore, the purpose of this study was to compare the anatomy of the intra-articular structures of the human knee to species commonly used in large animal research studies. METHODS: Fresh frozen cow (n=4), sheep (n=3), goat (n=4), dog (n=4), pig (n=5), rabbit (n=5), and human (n=4) cadaveric knees were used. Pass ...201121852139
survey on coenurosis in sheep and goats in egypt.a total of 75 sheep and goats from apparently healthy and from clinically affected flocks were examined for coenurus cerebralis cysts from different localities in egypt. of 25 animals examined from clinically diseased sheep and goats, 25 (100%) revealed the presence of infestation with one to four coenuri in the brain. the sites of predilection were the left hemisphere (48%), followed by the right hemisphere (40%) and the cerebellum (12%). there was no apparent effect of the age of sheep and goa ...201121947971
Accumulation of ammonia and other potentially noxious gases on live export shipments from Australia to the Middle East.Noxious gases on ships are irritant pollutants that have potential impacts on the comfort and health of both livestock and humans. Identification of environmental influences on the pollutants will assist live exporters to control them. Ammonia, hydrogen sulphide and carbon dioxide, as well as wet and dry bulb temperature, dew point, air speed and depth of faeces that the sheep stood in, were measured on two ship voyages in which sheep were transported from Australia to the Middle East. Daily mea ...201121912800
evaluation of cymbopogon schoenanthus essential oil in lambs experimentally infected with haemonchus contortus.hematophagous gastrointestinal parasites cause significant economic losses in small ruminant grazing systems. the growing reports of multi-drug resistant parasites call for intensive research on alternative treatments for anthelmintics to help small ruminants cope with these parasites. two-month-old lambs with mean body weight (bw) of 22.5kg were experimentally infected with a multidrug-resistant haemonchus contortus strain. infected animals were dosed orally with cymbopogon schoenanthus essenti ...201122206645
development of a loop-mediated isothermal amplification assay for rapid, sensitive and specific detection of a campylobacter jejuni clone.loop-mediated isothermal amplification (lamp) assay is a simple, rapid and specific detection method and has been used for detection and identification of different campylobacter species. in this study, we develop a lamp assay specific for detection of a particular clone (clone sa) of campylobacter jejuni, associated with the vast majority of recent sheep abortions in the u.s. using a set of specific primers for c. jejuni ia3902 (a clone sa isolate) and genomic dna or boiled cell extract as temp ...201122188995
bone marrow modified acrylic bone cement for augmentation of osteoporotic cancellous bone.the use of polymethylmethacrylate (pmma) cement to reinforce fragile or broken vertebral bodies (vertebroplasty) leads to extensive bone stiffening. this might be one reason for fractures at the adjacent vertebrae following this procedure. pmma with a reduced young's modulus may be more suitable. the goal of this study was to produce and characterize pmma bone cements with a reduced young's modulus by adding bone marrow. bone cements were produced by combining pmma with various volume fractions ...201122098908
evaluation of chondrocyte behavior in a new equine collagen scaffold useful for cartilage repair.association of biomaterials with autologous cells can provide a new generation of implantable devices for cartilage repair. an ideal scaffold should possess a preformed three-dimensional shape, fix the cells to the damaged area and prevent their migration into the articular cavity. furthermore, the constructs should have sufficient mechanical strength to facilitate handling in a clinical setting and stimulate the uniform spreading of cells and a phenotype re-differentiation process. the aim of t ...201122051171
Detection of PrPres in Genetically Susceptible Fetuses from Sheep with Natural Scrapie.Scrapie is a transmissible spongiform encephalopathy with a wide PrPres dissemination in many non-neural tissues and with high levels of transmissibility within susceptible populations. Mechanisms of transmission are incompletely understood. It is generally assumed that it is horizontally transmitted by direct contact between animals or indirectly through the environment, where scrapie can remain infectious for years. In contrast, in utero vertical transmission has never been demonstrated and ha ...201122194786
The Photobacterium damselae subsp. damselae hemolysins damselysin and HlyA are encoded within a new virulence plasmid.Photobacterium damselae subsp. damselae (formerly Vibrio damsela) is a marine bacterium that causes infections and fatal disease in a wide range of marine animals and in humans. Highly hemolytic strains produce damselysin (Dly), a cytolysin encoded by the dly gene that is lethal for mice and has hemolytic activity. We found that Dly is encoded in the highly hemolytic strain RM-71 within a 153,429-bp conjugative plasmid that we dubbed pPHDD1. In addition to Dly, pPHDD1 also encodes a homologue of ...201121875966
[Mastectomy in goats with inappropriate lactation syndrome].Objective: Hobby keeping of goats and sheep confronts veterinarians with new challenges that rarely have to be faced in livestock husbandry. During the last five years five goats were presented to the Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, Germany, with inappropriate lactation syndrome. Four of these animals had been previously treated with cabergoline without enduring success. According to the request of the owners (informed consent) and the c ...201122138742
[Epidemiological enquiries in two Q fever outbreaks in a community of Baden-Württemberg during 2008 and 2009].In 2008 and 2009, two consecutive outbreaks of Q fever in humans were recorded in the district of Freudenstadt, northern Black Forrest, Baden-Württemberg, Germany. In 2008, a total of 41 persons from a single local community fell ill and were found infected with Coxiella burnetii. Although comprehensive diagnostic and epidemiological outbreak investigations were conducted and control measures taken which included vaccination of ruminants at risk in three parts of the affected community, re-occur ...201121848037
Sensitive and specific detection of sporadic Creutzfeldt-Jakob disease brain prion protein using real-time quaking induced conversion.Real-time quaking induced conversion (RT-QuIC) is an assay in which disease-associated prion protein initiates a rapid conformational transition in recombinant prion protein resulting in the formation of amyloid that can be monitored in real time using the dye thioflavin T. It therefore has potential advantages over analogous cell-free prion protein conversion assays such as protein misfolding cyclic amplification (PMCA). The QuIC assay and the related amyloid seeding assay have largely been dev ...201122031526
Copper-induced hepatitis: the COMMD1 deficient dog as a translational animal model for human chronic hepatitis.Chronic inflammatory liver disease regardless of aetiology leads to failing regeneration and fibrosis, ending in cirrhosis. Both in man and in animals this worldwide health problem has no definitive cure. Chronic liver injury causes hepatic stellate cells to proliferate and differentiate into matrix-producing cells. New therapeutic options will be developed upon detailed understanding of the molecular mechanisms driving liver fibrosis. This may lead to new anti-fibrotic therapies which need to b ...201122029820
Regulation of Apoptotic Pathways by Stylophora pistillata (Anthozoa, Pocilloporidae) to Survive Thermal Stress and Bleaching.Elevated seawater temperatures are associated with coral bleaching events and related mortality. Nevertheless, some coral species are able to survive bleaching and recover. The apoptotic responses associated to this ability were studied over 3 years in the coral Stylophora pistillata from the Gulf of Eilat subjected to long term thermal stress. These include caspase activity and the expression profiles of the S. pistillata caspase and Bcl-2 genes (StyCasp and StyBcl-2-like) cloned in this study. ...201122194880
The interplay between gonadal steroids and immune defence in affecting a carotenoid-dependent trait.The hypothesis that sexual ornaments are honest signals of quality because their expression is dependent on hormones with immune-depressive effects has received ambiguous support. The hypothesis might be correct for those signals that are carotenoid-dependent because the required carotenoid deposition in the signal, stimulated by testosterone, might lower the carotenoid-dependent immune defence of the organism. Two pathways underlying this androgen-dependent honest signaling have been suggested. ...201121957328
Differences in extrinsic innervation patterns of the small intestine in the cattle and sheep.After oral challenge of the pathological prion protein, the pathogen was first detected in the distal ileum and then deposited in the brain. The present study aims determining the possible neuronal transport pathways from the small intestine to the brain in the cattle and sheep using a tracer protein. After horseradish peroxidase was injected into the wall in the duodenum of the calf and lamb and in the ileum of the lamb, the greater part of labeled neurons was detected in the celiac and cranial ...201122209656
Apelin is involved in postprandial responses and stimulates secretion of arginine-vasopressin, adrenocorticotropic hormone, and growth hormone in the ruminant.Apelin and its mRNA are expressed in several tissues, including the supraoptic and paraventricular nuclei in the hypothalamus. Although apelin is reported to be involved in the regulation of fluid homeostasis, little is known about the postprandial dynamics of apelin in plasma and its regulatory effects on the anterior pituitary hormones of ruminants. Therefore, the aims of this study were to investigate the following: (1) changes in plasma apelin concentrations in response to food intake under ...201122177697
A novel role for an endothelial adrenergic receptor system in mediating catecholestradiol-induced proliferation of uterine artery endothelial cells.Sequential conversion of estradiol-17ß to its biologically active catecholestradiols, 2-hydroxyestradiol (OHE(2)) and 4-OHE(2), contributes importantly to its angiogenic effects on uterine artery endothelial cells (UAECs) derived from pregnant, but not nonpregnant ewes via an estrogen receptor-independent mechanism. Because catecholestradiols and catecholamines exhibit structural similarities and have high affinity for a- and ß-adrenergic receptors (ARs), we investigated whether the endothelial ...201121947467
gene cloning, expression, and localization of antigen 5 in the life cycle of echinococcus granulosus.antigen 5 (ag5) has been identified as a dominant component of cyst fluid of echinococcus granulosus and is considered as a member of serine proteases family, which in other helminth, plays an important role in the egg hatch and larva invasion. however, whether ag5 is expressed and secreted in all life stages is unknown. in this study, according to the sequence in genbank, we cloned and sequenced the open reading frame (orf) of ag5 gene from the protoscolices of e. granulosus isolated from the s ...201122200957
The effects of flumethrin (Bayticol® pour-on) on European ticks exposed to treated hairs of cattle and sheep.Tick infestations in cattle and sheep pose serious health problems when agents of diseases are transmitted. In addition, blood feeding of ticks induces enormous economic losses due to reduced weight gain of infested animals. The present study was designed to investigate the effects of exposure to hairs clipped from cattle and sheep treated with flumethrin (Bayticol®) on European ticks. The dose used was 10 ml/100 kg body weight for both animal species. At intervals of 7 days (days 7, 14, 21, 28 ...201122205348
Neurological diseases of ruminant livestock in Australia. IV: viral infections.Most viral infections that affect the central nervous system of ruminants are exotic to Australia. As such, this review focuses on viruses of importance in Australian ruminants, including Akabane virus and the ruminant pestiviruses, bovine viral diarrhoea virus and border disease virus, as well as bluetongue virus. Each virus is discussed in terms of pathogenesis, clinical signs and diagnosis.201121864304
The effect of intermittent umbilical cord occlusion on elastin composition in the ovine fetus.This study aimed to determine the effect of varying degrees of intermittent umbilical cord occlusion (UCO) on arterial elastin composition. Over 4 days, chronically catheterized late gestation fetal sheep received 5 total UCO per day lasting 1 min/h (mild group: n = 6), 2 min/h (moderate group: n = 4), 3 min/h (severe group; n = 6); or no occlusion (control group: n = 7). Each group was evaluated for elastin content of the carotid and superior mesenteric artery (SMA), the arterial pressure respo ...201121960511
Novel anti-inflammatory effects of repaglinide in rodent models of inflammation.Background: Repaglinide is an FDA-approved treatment for type 2 diabetes mellitus. The anti-inflammatory effect of repaglinide in the absence of diabetes has not been reported previously. It is the objective of this set of studies to investigate the potential anti-inflammatory effects of repaglinide. Method: The in vivo anti-inflammatory effects of repaglinide were studied in two different models of delay type hyperreactivity (DTH) response induced by sheep red blood cells (sRBC) and 2,5'-dinitr ...201122086064
haemodynamic responses to angiotensin ii in conscious lambs: role of nitric oxide and prostaglandins.it was hypothesized that nitric oxide (no) and prostaglandins (pgs) play a synergistic role in modulating haemodynamic responses to angiotensin ii (ang ii) in an age-dependent manner. to this end, experiments were carried out in conscious, chronically instrumented lambs aged ∼1 week (n = 9) and ∼6 weeks (n = 10) to evaluate the haemodynamic responses to ang ii, before and after treatment with the l: -arginine analogue, n-nitro-l: -arginine methyl ester (l: -name), as well as the cyclooxygenase i ...201122170097
Methanotrophic community structure and activity under warming and grazing of alpine meadow on the Tibetan Plateau.Knowledge about methanotrophs and their activities is important to understand the microbial mediation of the greenhouse gas CH(4) under climate change and human activities in terrestrial ecosystems. The effects of simulated warming and sheep grazing on methanotrophic abundance, community composition, and activity were studied in an alpine meadow soil on the Tibetan Plateau. There was high abundance of methanotrophs (1.2-3.4?×?10(8) pmoA gene copies per gram of dry weight soil) assessed by real-t ...201121847510
Masculinization of the Distal Tubular and External Genitalia in Female Sheep With Prenatal Androgen Exposure.Prenatal exposure to endogenous or exogenous androgens alters the development of the female reproductive tract. Although lesions in ovaries and external genitalia of androgenized female sheep have been reported, lesions of the tubular genitalia have not. Testosterone propionate (TP) or dihydrotestosterone (DHT) was administered by intramuscular injection twice weekly to 32 ewes from 30 to 90 days of pregnancy. The ewes lambed normally. The reproductive tracts from 24 treated and 13 control postp ...201121934102
molecular modeling: a search for a calpain inhibitor as a new treatment for cataractogenesis.studies of 17 analoges of 3 (sja6017) in an in silico calpain model are reconciled to measured ic(50) values against ovine calpain. the studies validate the potential of the "model" and criteria established for inhibition as a tool to select structures for synthesis to test as calpain inhibitors. using this screening methodology of virtual libraries led us to synthesize several inhibitors including macrocycle 33, which in vitro sheep eye lens culture experiments showed to substantially slow opac ...201121955158
cases of bacterial pneumonia in cattle and sheep. 201121870358
BSE: where are we now? 201121965237
visna/maedi virus genetic characterization and serological diagnosis of infection in sheep from a neurological outbreak.an extensive outbreak characterized by the appearance of neurological symptoms in small ruminant lentivirus (srlv) infected sheep has been identified in spain, but the genetic characteristics of the strain involved and differential diagnostic tools for this outbreak remain unexplored. in this work, 23 visna-affected naturally infected animals from the outbreak, 11 arthritic animals (both groups presenting anti-visna/maedi virus serum antibodies), and 100 seronegative animals were used. eight of ...201121940116
Pulmonary vascular effects of serotonin and selective serotonin reuptake inhibitors in the late-gestation ovine fetus.Maternal use of selective serotonin (5-HT) reuptake inhibitors (SSRIs) is associated with an increased risk for persistent pulmonary hypertension of the newborn (PPHN), but little is known about 5-HT signaling in the developing lung. We hypothesize that 5-HT plays a key role in maintaining high pulmonary vascular resistance (PVR) in the fetus and that fetal exposure to SSRIs increases 5-HT activity and causes pulmonary hypertension. We studied the hemodynamic effects of 5-HT, 5-HT receptor antag ...201121908589
Effects of different mandibular fracture patterns on the stability of miniplate screw fixation in angle mandibular fractures.The aim of the present study was to evaluate the effects of horizontally favourable and unfavourable mandibular fracture patterns on the fixation stability of titanium plates and screws by simulating chewing forces. Favourable and unfavourable mandibular fractures on 22 sheep hemimandibles were fixed with 4-hole straight titanium plates and 2.0mm×7mm titanium screws according to the Champy technique. Hemimandibles were mounted with a fixation device in a servohydraulic testing unit for compressi ...201122178275
Evaluation of the effects of guided bone regeneration and periosteum on newly formed bone in a distraction gap.We determined the causes of bone resorption within a distraction gap and determined whether it could be prevented via guided bone regeneration during distraction. Another goal was to determine the effect of periosteum in bone healing in a distraction gap.Twelve sheep mandibles were bilaterally distracted. One side of the 6 sheep mandibles formed the control group; the other side was the study group, from which the periosteum was excised and distraction was performed. In the other 6 sheep, on the ...201121959452
Functional factor VIII made with von Willebrand factor at high levels in transgenic milk.Current manufacturing methods for recombinant human factor VIII (rFVIII) within mammalian cell cultures are inefficient, hampering the production of sufficient amounts for affordable, worldwide treatment of hemophilia A. However, rFVIII has been expressed at very high levels by the transgenic mammary glands of mice, rabbits, sheep, and pigs. Unfortunately, it is secreted into milk with low specific activity, owing in part to the labile, heterodimeric structure that results from furin processing ...201121920013
wormkill to wormboss-can we sell sustainable sheep worm control?despite the economic importance of nematodes for sheep enterprises, it is often difficult to convince producers to adopt best practice in worm control. in this paper we discuss key issues in effecting change by communicating worm control messages. the adoption of new strategies is affected by many factors including the relative advantage, complexity, compatibility and the ability to trial the proposed change. the visibility of the problem to be solved and proposed positive outcome are also impor ...201122153257
Identification, structure, and characterization of an exopolysaccharide produced by Histophilus somni during biofilm formation.Histophilus somni, a gram-negative coccobacillus, is an obligate inhabitant of bovine and ovine mucosal surfaces, and an opportunistic pathogen responsible for respiratory disease and other systemic infections in cattle and sheep. Capsules are important virulence factors for many pathogenic bacteria, but a capsule has not been identified on H. somni. However, H. somni does form a biofilm in vitro and in vivo, and the biofilm matrix of most bacteria consists of a polysaccharide.201121854629
Novel intracranial brain cooling catheter to mitigate brain injuries.Background The neuroprotective effects of cooling the spinal cord in a sheep model by a self-contained intrathecal catheter was reported recently by the authors. The present study was designed to determine if cooling catheters in the lateral ventricles of the brain can effectively cool the CSF and thereby reduce brain temperature while maintaining systemic normothermia. Methods The cooling catheter is a self-contained system that circulates a cold fluid and cools the CSF that circulates in the ...201121990455
Gastrointestinal nematode infections in German sheep.The objective of the present study was to determine the prevalence and variation of natural gastrointestinal nematode (GIN) infections in lambs according to birth type, gender and breed based on individual faecal egg counts (FEC) from various regions in Germany. A total of 3,924 lambs (3 to 15 months old) with different genetic backgrounds (Merinoland, German Blackhead Mutton, Rhoen, Texel and Merino long-wool) were individually sampled during the grazing period between 2006 and 2008. Furthermor ...201121979784
Effect of selenium supplementation with sodium selenite and selenium nanoparticles on iron homeostasis and transferrin gene expression in sheep: A preliminary study.The present research aimed at evaluating the effects of sodium selenite and selenium nanoparticles (Se NPs) on iron homeostasis and the expression of transferrin and its receptor-binding protein genes. Twenty one Lori-Bakhtiary sheep were randomly allocated into 3 groups. Groups 1 and 2 orally received Se NPs and sodium selenite (1mgkg(-1)) for 10 consecutive days, respectively. Group 3 served as the control. Blood and sternal bone marrow samples were collected at different supplementation inter ...201121868048
Effects of shearing and fleece cleanliness on microbiological contamination of lamb carcasses.The meat industry in Norway has developed national guidelines for Good Hygiene Practices for slaughtering and skinning, based on categorisation of animals. These include shearing sheep and lambs in the abattoirs immediately before slaughter. The aim of this study was to investigate microbiological carcass contamination associated with: (i) different shearing regimes; (ii) fleece cleanliness; and (iii) the slaughter process. In addition, the efficacy of the national guidelines in reducing microbi ...201121862163
purification of carbonic anhydrase-ii from sheep liver and inhibitory effects of some heavy metals on enzyme activity.in this study; sheep carbonic anhydrase-ii (sca-ii) (e.c: 4.2.1.1) was purified from sheep liver and in vitro effects of heavy metals on the enzyme was examined. sca-ii isozyme was purified with about 203 purification fold, a specific activity of 2320 eu/mg-protein and a yield of 72 by using sepharose-4b-l tyrosine-sulfanilamide affinity gel chromatography. purity of the sca-ii enzyme was verified by sds-page technique and subunit molecular mass of the enzyme was found as 29 kda. in addition to ...201121985503
all clinically-relevant blood components transmit prion disease following a single blood transfusion: a sheep model of vcjd.variant cjd (vcjd) is an incurable, infectious human disease, likely arising from the consumption of bse-contaminated meat products. whilst the epidemic appears to be waning, there is much concern that vcjd infection may be perpetuated in humans by the transfusion of contaminated blood products. since 2004, several cases of transfusion-associated vcjd transmission have been reported and linked to blood collected from pre-clinically affected donors. using an animal model in which the disease mani ...201121858015
arginine nutrition and fetal brown adipose tissue development in nutrient-restricted sheep.intrauterine growth restriction is a significant problem worldwide, resulting in increased rates of neonatal morbidity and mortality, as well as increased risks for metabolic and cardiovascular disease. the present study investigated the role of maternal undernutrition and l: -arginine administration on fetal growth and development. embryo transfer was utilized to generate genetically similar singleton pregnancies. on day 35 of gestation, ewes were assigned to receive either 50 or 100% of their ...201122130738
assessment of peripheral airway function following chronic allergen challenge in a sheep model of asthma.there is increasing evidence that the small airways contribute significantly to the pathophysiology of asthma. however, due to the difficulty in accessing distal lung regions in clinical settings, functional changes in the peripheral airways are often overlooked in studies of asthmatic patients. the aim of the current study was to characterize progressive changes in small airway function in sheep repeatedly challenged with house dust mite (hdm) allergen.201122174883
high-resolution in utero 3d mr imaging of inner ear microstructures in fetal sheep.developmental inner ear abnormalities can occur due to embryopathies as well as in the context of syndromal diseases like the charge association. in severe cases, an early and definite in utero diagnosis is important for decision-making; here, fetal mr imaging can be a helpful tool. we present results of performing high-resolution mr imaging of the inner ear structures of fetal sheep in vivo.201122033721
seroprevalence of toxoplasma gondii in domestic and wild animals from the fernando de noronha, brazil.abstract fernando de noronha is an archipelago of 21 islands and islets in the atlantic ocean, state of pernambuco, brazil, and has a varied biodiversity, including alien species or sinantropic animals. the objective here was to determine seroprevalence of toxoplasma gondii in domestic and wild animals from fernando de noronha archipelago, brazil. between july 2007 to may 2010, blood samples were collected from 764 animals (533 domestic and 231 wild animals). sera were tested by the indirect f ...201122150091
genetic diversity of ovis aries populations near domestication centers and in the new world.domestic sheep in kazakhstan may provide an interesting source of genetic variability due to their proximity to the center of domestication and the silk route. additionally, those breeds have never been compared to new world sheep populations. this report compares genetic diversity among five kazakhstan (kz) and 13 united states (us) sheep breeds (n = 442) using 25 microsatellite markers from the fao panel. the kz breeds had observed and expected measures of heterozygosity greater than 0.60 and ...201122105875
A survey of helminth control practices on sheep farms in Great Britain and Ireland.A telephone survey of 600 farmers throughout Great Britain and Ireland was conducted in order to characterise helminth control practices, and identify factors correlated with perceived anthelmintic failure. Overall, 93% of surveyed farmers routinely treated their sheep against nematodes, 67% against liver fluke and 58% against tapeworms. Anthelmintic resistance in nematodes was perceived by farmers to be present on 10% of farms. Farmers who dosed ewes at mating were more likely to have observed ...201121908211
Structural analysis of effector functions related motifs, complement activation and hemagglutinating activities in Lama glama heavy chain antibodies.Heavy chain antibodies (HCAbs), devoid of the light chains and the CH(1) domain, are present in the serum of camelids. IgG(2) and IgG(3) are HCAbs; whereas IgG(1) has the conventional structure. In order to study the immunological properties of llama HCAbs, from which to date little is known, llamas (Lama glama) HCAbs cDNA were cloned, sequenced and compared with other mammalian Igs. The sequence analysis showed that llama HCAbs cDNA organization is similar to other mammalian Igs and the presenc ...201122197565
ileal peyer's patches are not necessary for systemic b cell development and maintenance and do not contribute significantly to the overall b cell pool in swine.based on studies of sheep, ileal peyer's patches (ipp) have been regarded as a type of primary lymphoid tissue similar to the bursa of fabricius in chicken. because bursectomy results in b cell deficiency, we wondered whether resection of the ipp of piglets would have a similar effect. comparison of ipp-resected, surgical shams and untreated germ-free piglets, all of which were later colonized with a defined commensal flora, demonstrated that resection of the ipp did not alter the level and phen ...201122013120
effects of lairage time after road transport on some blood indicators of welfare and meat quality traits in sheep.the study was conducted to evaluate the effects of different lairage time after 8 h road transport on some blood indicators of welfare and meat quality traits in sheep. a total of 84 ujimqin male sheep (average body weight 27.5 kg, 6 months old) were randomly allotted to one of seven groups: one control group (untransported) and six lairage groups (8 h road transport with 0, 2, 6, 12, 24 and 48 h lairage times respectively). no significant lairage time effects were observed on weight loss. sheep ...201121929728
double-plating of ovine critical sized defects of the tibia: a low morbidity model enabling continuous in vivo monitoring of bone healing.recent studies using sheep critical sized defect models to test tissue engineered products report high morbidity and complications rates. this study evaluates a large bone defect model in the sheep tibia, stabilized with two, a novel carbon fibre poly-ether-ether-ketone (cf-peek) and a locking compression plate (lcp) which could sustain duration for up to 6 month with an acceptable low complication rate.201121958221
Paraffin-embedded tissue blot as a sensitive method for discrimination between classical scrapie and experimental bovine spongiform encephalopathy in sheep.The paraffin-embedded tissue (PET) blot was modified for use as a tool to differentiate between classical scrapie and experimental bovine spongiform encephalopathy (BSE) in sheep. Medulla (obex) from 21 cases of classical scrapie and 6 cases of experimental ovine BSE were used to develop the method such that it can be used as a tool to differentiate between BSE and scrapie in the same way that differential immunohistochemistry (IHC) has been used previously. The differential PET blot successfull ...201121908277
Biomechanical evaluation of malleable noncompression miniplates in mandibular angle fractures: an experimental study.The purpose of this experimental study was to test the reliability of a single malleable titanium miniplate using Champy's method of fixing fractures of the mandibular angle. Eighteen sheep hemimandibles were used to evaluate 2 plating techniques. The groups were tested with either a single non-compression titanium miniplate or a single malleable titanium miniplate. A cantilever bending biomechanical test model was used for the samples. Each group was tested with vertical forces using a servohyd ...201122118919
Structural and biological characterization of mastoparans in the venom of Vespa species in Taiwan.Mastoparans, a family of small peptides, are isolated from the wasp venom. In this study, six mastoparans were identified in the venom of six Vespa species in Taiwan. The precursors of these mastoparans are composed of N-terminal signal sequence, prosequence, mature mastoparan, and appendix glycine at C-terminus. These mature mastoparans all have characteristic features of linear cationic peptides rich in hydrophobic and basic amino acids without disulfide bond. Therefore, these peptides could b ...201121884742
Tracing genetic differentiation of Chinese Mongolian sheep using microsatellites.The genetic consequences of population differentiation and isolation have been the subject of conservation biology. In this study, we analysed the genetic diversity and structure of Mongolian sheep in China. These animals belong to a traditional local breed with high production, extensive adaption, early maturity and roughage resistance. For this purpose, 26 microsatellites were genotyped for five Mongolian sheep populations. The Bayesian clustering indicated five clusters as the most probable g ...201121906110
Effect of the administration program of two beta-adrenergic agonists on growth performance, carcass, and meat characteristics of feedlot ram lambs.The aim of the study was to determine the effects of three feeding dose-programs of the ß-adrenergic agonists (ß-AA) ractopamine hydrochloride (RH) or zilpaterol hydrochloride (ZH) for the final 30 d before harvest, on growth performance, carcass and meat characteristics of feedlot ram lambs. Eighty-four Dorper x Katahdin ram lambs (30.0 ± 1.6 kg) were blocked by weight and randomly assigned to pens (four lambs per pen and three pens per treatment). Pens within a block were assigned randomly to ...201122147472
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