| electron microscopic mapping of proteins bound to herpes simplex virus dna. | exonuclease digestion experiments have suggested that there is a protein(s) bound close to one or both ends of herpes simplex virus-1 (hsv) dna. the existence of such bound proteins has been positively demonstrated and their positions on the hsv genome determined by application of a newly developed method for electron microscopic mapping of proteins bound to nucleic acids. purified hsv dna was treated with dinitrofluorobenzene under conditions that covalently attach the dinitrophenyl (dnp) group ... | 1979 | 226938 |
| susceptibility of mink to certain viral animal diseases foreign to the united states. | mink (mustela vison) were inoculated with viruses: african horse sickness (ahs), african swine fever (asf), bovine herpes virus ii (bhv2), foot-and-mouth disease (fmd), goat pox (gp), hog cholera (hc), peste des petits ruminants (ppr), rinderpest (rp), swine vesicular disease (svd), vesicular exanthema of swine (ves) and vesicular stomatitis (vs). their susceptibility was measured by development of clinical signs, virus isolation and detection of precipitin and/or virus neutralizing antibodies. ... | 1979 | 228092 |
| pas-positive inclusions in the alveolar type ii cells of the caprine lung. | histochemical analysis of inclusions in the alveolar type ii cells was done with the alcian blue-pas technique. pas-positive inclusions, which were absent in the sheep and buffalo, were detected in the alveolar type ii cells of the goat lung. mixed diamine-sodium chloride did not stain these inclusions which also showed orthochromatic blue reaction with toluidine blue. the high percentage of alveolar type ii cells showing these inclusions suggested that this difference could be explained by the ... | 1979 | 228366 |
| the effect of dietary composition on the binding of insulin and glucagon to goat hepatocytes [proceedings]. | | 1979 | 229040 |
| incidence of bluetongue virus precipitating antibodies in sera of some domestic animals in the sudan. | to determine the presence and prevalence of bluetongue (bt) infection in a variety of domestic animal species in different geographical regions of the sudan, a serological study using the agar gel precipitation technique was initiated. a total of 2142 serum samples were examined. of the numbers tested approximately 28% of sheep, 11.2% of goats, 8% of cattle and 4.9% of camels were positive for group-specific antibodies to bt virus antigen, indicating previous exposure to bt infection. none of th ... | 1979 | 229163 |
| 'aspira': a new sensitive assay for the detection of hepatitis b surface antigen. | a sensitive method for the detection of hepatitis b surface antigen (hbsag) is described whereby the separation of specific antibody by affinity chromatography is incorporated into one of the assay reagents. hbsag, which may be obtained from tissue culture fluids of a human hepatoma cell line, is first reacted with polystyrene beads. unoccupied spaces on the plastic are next covered with a non-specific protein (e.g. albumin). the plastic is then reacted with either whole antiserum or an igg frac ... | 1979 | 230261 |
| immunofluorescent localization of angiotensin converting enzyme in epithelioid and giant cells of sarcoidosis granulomas. | angiotensin converting enzyme (ace) (dipeptidyl carboxypeptidase; peptidyldipeptide hydrolase, ec 3.4.15.1) was localized in epithelioid and giant cells within ace-rich sarcoidosis granulomas, but not control granulomas, by immunofluorescence by using a rabbit anti-human ace immunoglobulin for localization and fluorescein-labeled goat anti-rabbit immunoglobulin for detection. ace was frequently not detected in the very central epithelioid cells, perhaps due to a paucity of cellular or molecular ... | 1979 | 230518 |
| endocrinology of lactation. | hormones are necessary for the growth of mammary glands, for initiation of the secretory process, and for the maintenance of an established lactation in all mammals. hormonal changes which occur in late pregnancy and the early postpartum period are described and graphed. this data was derived from research with animal models and relevant human data. enzymatic and cytologic differentiation of the mammary gland and limited mammary secretion begin during late pregnancy. this constitutes the 1st ... | 1979 | 230600 |
| experimental infection of goats with parainfluenza virus type 3. | | 1979 | 230666 |
| antibody to rotavirus in various animal species. | | 1979 | 231743 |
| [herpesvirus infection in kids in switzerland]. | | 1979 | 231824 |
| [cysticercus in animals slaughtered in the port-bouet abattoir (abidjan)]. | | 1978 | 232291 |
| chemically modified porous silica gel as a bioadsorbent and a biocatalyst. | | 1979 | 232385 |
| isolation of parapox viruses from man and animals: cultivation and cellular changes in bovine fetal spleen cells. | | 1979 | 232437 |
| analysis of nonspecific reactions in enzyme-linked immunosorbent assay testing for human rotavirus. | solid-phase enzyme immunoassays can be utilized to detect antigens directly in clinical specimens. however, a small number of stools which we tested for human rotavirus by enzyme-linked immunosorbent assay (elisa) were found to have nonspecific activity in the absence of rotaviral antigen. similar nonspecific activity was found in eight of eight sera which contained rheumatoid factor. this nonspecific activity was markedly reduced by pretreatment of the specimens with reducing agents, normal goa ... | 1979 | 232506 |
| isolation and identification of the sersenk strain of goat pox virus in iraq. | goat pox virus was isolated during an outbreak of pox infection among goats in the sersenk district, iraq. the isolated virus grew on the chorioallantoic membranes of developing chick embryos and in primary lamb testis cell cultures. it was identified morphologically as a pox virus and serologically as a member of the capripoxvirus group in the family poxviridae. the isolated virus was designated the "sersenk" strain. | 1979 | 233273 |
| immunochemical evidence for glutamine-mediated degradation of glutamine synthetase in cultured chinese hamster cells. | the specific activity of glutamine synthetase in cultured chinese hamster cells is inversely related to the concentration of glutamine in the surrounding solution. enzyme specific activity increases 8- to 10-fold when glutamine is removed from serum-free f12 growth media. the induction of glutamine synthetase activity occurs only after glutamine removal and not after the removal of other amino acids (methionine, leucine, or isoleucine). the analysis of the glutamine-mediated decrease in glutamin ... | 1975 | 234454 |
| chlorpheniramine-dependent thrombocytopenia. | | 1975 | 234545 |
| volume, adherence properties, membrane antigens and mitogen responsiveness of rabbit lymphoid cell subpopulations. | subpopulations of rabbit spleen cells which respond to t and b mitogens, respectively, can be distinguished by sedimentation velocity in the earth's gravitational field. t cell subpopulations which differed in their responsiveness to con a and to pha could be identified by differences in adherence properties and by their sensitivity to complement mediated cell kill with rtla-antiserum. | 1975 | 234909 |
| placental transfer and fetal urinary excretion of gentamicin during constant rate maternal infusion. | placental transfer and fetal urinary excretion of gentamicin was studied in midtrimester goat and human previable fetuses during constant rate maternal infusion with the drug. gentamicin was not detected in the serum of any of the goat fetuses, even when maternal serum concentrations ranged from 15.2 mug/ml to 20.9 mug/ml. however, gentamicin was present in the amniotic fluid of four animals. gentamicin was also present in fetal urine collected from three animals. in contrast human fetal central ... | 1975 | 235108 |
| pharmacological receptors of the cerebral arteries of the goat. | 5-hydroxytryptamine (5-ht), norepinephrine (ne), histamine (h) and potassium (k+) chloride induce dose-dependent changes in tension of the isolated middle crerbral artery of the goat. vasopressin produces highly variable responses followed by tachyphylaxis; angiotensin ii is ineffective over a wide dose range. the order of potencies of these vasoactive agents is 5-ht greater than ne greater than h greater than k+. with regard to their ability to induce maximal contractile responses, the order is ... | 1975 | 235340 |
| the presence of collagenase in collagen preparations. | the frequently observed instability of neutral salt solutions of native collagen extracted from various sources and partially purified by standard procedures has been studied by disc electrophoresis in polyacrylamide gel and by electron microscopic examination of segment long spacing crystallites. the phenomenon has revealed time and temperature dependency, ph optima near neutrality, and inhibition by sodium edta and serummin addition, collagen breakdown has been found to be quantitatively relat ... | 1975 | 236042 |
| galactose-6-phosphate dehydrogenase. purification and partial characterization. | a new enzyme, galactose-6-phosphate dehydrogenase has been purified about 50-fold from goat liver. the enzyme can be distinguished from the nonspecific hexose-6-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase by its high substrate specificity and absolute pyridine nucleotide requirement. in contrast to the hexose-6-phosphate dehydrogenase, this enzyme is located exclusively in the cytoplasmic fraction of the cell. the enzyme is a metalloprotein and is highly sensitive to mercurials ... | 1975 | 236296 |
| the effect of anemia on the ventilatory response to transient and steady-state hypoxia. | the effects of anemia upon the ventilatory responses to transient and steady-state hypoxia were studied in unanesthetized goats. responses to transient hypoxia (inhalation of several breaths of nitrogen) were considered to reflect peripheral chemoreceptor and non-chemoreceptor influences of hypoxia upon ventilatory control. in all goats, severe anemia (hemoglobin 3.1-4.8 g/100ml) markedly heightened the responses to transient hypoxia (from a mean of 0.27 to a mean of 0.75 liter/min/percent fall ... | 1975 | 236329 |
| the distribution and movements of carbon dioxide, carbonic acid and bicarbonate between blood and milk in the goat. | 1. a-v differences and milk concentrations of respiratory gases, ph, hco3 and h2co3 have been measured in lactating goats and cows. 2. the ph and [hco3 minus] of milk were significantly lower than those of plasma while milk pco2 was virtually identical to that of mammary venous blood. [h2co3+ dissolved co2] was similar in milk and blood. 3. 14-c (from injected [14-c]hco3 minus was found to cross the mammary epithelium in both directions. 14-c also passed across the duct epithelium and since this ... | 1975 | 237118 |
| experimental and theoretical aspects of protein folding. | | 1975 | 237413 |
| comparative micelle structure. iv. the similarity between caprine alphas-casein and bovine alphas-3- casein. | the major component of caprine (goat) alphas-casein has been isolated by deae-and cm-cellulose chromatography in buffers containing urea and 2-mercaptoethanol. the protein has a molecular weight of 25700 as determined by gel filtration on sepharose 6b in guanidine hydrochloride. its composition, asp17, thr14, ser14, glu45, pro18, gly4, ala10, cys2, val12, met4, ile12, leu12, tyr11, phe8, his5, lys22, arg6, trp2 and 7 phosphate residues, is much closer to that of bovine alphas3-casein than to bov ... | 1975 | 237571 |
| intrinsic and chemically produced microheterogeneity of staphylococcus aureus enterotoxin type c. | staphylococcus aureus enterotoxins c1 (sec1) and c2 (sec2) produced from 50-liter quantities of crude culture supernatants were purified chromatographically in a neutral or acid milieu. microheterogenity of sec1 was markedly increased by treatment of the purified toxin with alkali, and new more acidic charged species appeared. sec2 was more heterogenous than any of the other s. aureus enterotoxins and was affected only slightly by treatment with alkali. prolonged incubation of the organism durin ... | 1975 | 237837 |
| acid phosphatases activity in cheese and starters. | the acid phosphatase activity levels in a number of greek cheeses and in cheddar cheeses were found to be unaffected by storage for up to 18 months and 12 months respectively. in cheddar cheese, starter organisms made an insignificant contribution to this activity. studies of acid phosphatase prepared from streptococcus cremoris-lactis ncdo 762 starter cultures showed that the enzyme was of high molecular weight and largely particle-bound. the ph of optimum activity was 5-2 and the enzyme was in ... | 1975 | 237943 |
| serologic evidence of venezuelan equine encephalitis in some wild and domestic populations of southern texas. | more than 2,500 sera from approximately 30 wild and domestic species in southern texas were tested for neutralizing antibodies to venezuelan equine encephalitis (vee). virus isolations were also attempted from blood and tissue samples of many of the wild specimens. vee neutralizing substances were present in a variety of species collected prior to the 1971 epizootic, suggesting that vee was present and perhaps enzootic in this area before the recent epizootic. serologic results of this study sug ... | 1975 | 238050 |
| studies on stimulation and properties of antiglobulin antibodies. ii. antiglobulin antibodies against homologous globulins and antigens of thermally denatured autologous sera in the sera of rabbits immunized with various antigens. | sera of 48 rabbits after long-term immunization with suspensions of diplococcus pneumoniae, klebsiella friedländeri, proteus ox19 and solution of bovine albumin were examined for antibodies reacting with homologous gamma-globulins and antigens of thermally denatured autologous sera. appearance of antibodies of this type was related to duration of immunization, but not related to the type of antigen used. the experimental results were explained as a result of altered antigenic properties of antib ... | 1975 | 238486 |
| transplantation in severe combined immunodeficiency disease with hl-a identical bone marrow. | the immunologic reconstitution of 7 patients with severe combined immunodeficiency disease was attempted with bone marrow transplantation from histoidentical donors. four patients were successfully reconstituted and discharged from the hospital. two patients died with sepsis. one patient died from a preexisting neurologic disease. all the patients who have been successfully reconstituted have had some degree of graft-vs-host disease. a dose of 50 x 10(6) nucleated bone marrow cells per kg seems ... | 1975 | 238684 |
| effect of liver failure on the response of ventilation and cerebral criculation to carbon dioxide in man and in the goat. | 1. the acid-base state of arterial blood and cerebrospinal fluid, and the ventilatory response to co2, were measured in twelve patients with liver disease. the co2 response was also measured in eight goats before and after the experimental production of liver failure. arterial pco2 and ph, cerebral blood flow and the cerebral metabolic rate for oxygen were also measured in four of the goats while they breathed air and various co2-enriched gas mixtures. 2. liver failure was accompanied by a respi ... | 1975 | 238783 |
| the existence during gestation of an immunological buffer zone at the interface between maternal and foetal tissues. | in mammalian pregnancy the trophoblast normally constitutes an uninterrupted boundary of foetal tissue in immediate contact with maternal tissue, including blood in some species, and is the decisive immunological barrier to rejection of the foetus as an allograft. the ability of the trophoblast to function as a barrier evidently results from its capacity to resist immunological attack by either alloantibody or alloimmune cells and to prevent immunocompetent cells from reaching and damaging the f ... | 1975 | 239427 |
| blood oxygen affinity and alveolar ventilation in relation in body weight in mammals. | the validity of the concept relating blood oxygen affinity and alveolar ventilation to body weight in homeothermic mammals was reexamined with blood ph used as the fixed variable. blood po2 at 50% saturation at ph 7.4 (p50(7.4)) and pco2 of oxygenated blood at ph 7.4 (pco2(7.4)) from a variety of homeothermic mammals were determined at 37 degrees c by in vitro equilibration techniques. in some species, arterial pco2, po2, and ph were also measured. pco2(7.4), which was similar to arterial pco2, ... | 1975 | 240284 |
| folic acid conjugase from plasma. i. partial purification and properties. | human plasma conjugase was partially purified by ammonium sulphate and deae-cellulose column fractionation. the ph optimum was found to be 4.5. the enzyme followed normal michaelis-menten kinetics. the km value was found to be 1.4 x 10(-7)m for folylheptaglutatmate assuming this compound to be the predominant form of folate in yeast extract. the enzyme required sulfhydryl compounds for full activity. differential microbiological assay of the conjugase reaction product using l. casei and s. faeca ... | 1975 | 240785 |
| chimaerism of immunocompetent cells in allogeneic bone marrow-reconstituted lethally irradiated chickens. | injection of parental bone marrow cells into 12-day-old lethally irradiated f1 hybrid chickens resulted in chimaerism of donor-type graft-versus-host (gvh)-reactive cells and suppression of antisheep red blood cell antibody response. these manifestations of a chronic graft-versus-host reaction were prevented by pretreatment of the donor marrow with specific anti-t cell globulin. in some chimaeras donor-type gvh-reactive cells developed gradually from t cells precursors of donor origin. transplan ... | 1975 | 241144 |
| folic acid conjugase from plasma. iii. use of the enzyme in the estimation of folate activity in foods. | a convenient method for the estimation of food folates using human plasma as a source of folic acid conjugase has been standardized. bovine, goat and dog plasma could not be used for the purpose because of low enzyme activity and slow reaction rates. human plasma conjugase released food folates from conjugated forms over a ph range of 4.5 to 6.0. the food folate values obtained by employing rat liver and chicken pancreas conjugase closely agreed with the values obtained by using human plasma con ... | 1975 | 241729 |
| the autoregulatory capability of galen's rete cerebri and its connections. | the rete, a vascular system between the extra- and intracerebral arteries of certain animals, may have a variety of functions in different species. the calibres of the vessels of the rete and its arteries of supply have been measured under different conditions of blood pressure and paco2. it has been shown that the rete and its afferent arteries behave in an autoregulatory fashion down to a blood pressure of 60 mmhg. they also dilate with a rise in paco2. it has also been found that some parts o ... | 1975 | 241946 |
| effect of volatile fatty acids on water and ion absorption from the goat colon. | the absorption of volatile fatty acids (vfa) and the influence of vfa on the net transport of inorganic electrolytes and water were examined in the temporarily isolated colon of the conscious goat. perfusion of the colon with a solution similar in content to that normally present in the cecal outflow showed that the net absorption of vfa was more rapid than that of any other ion present in the solution. when the vfa were replaced with cl, the net absorption of na and water was reduced nearly two ... | 1975 | 242224 |
| optical properties of bilirubin-serum albumin complexes in aqueous solution. a comparison among albumins from different species. | | 1975 | 242257 |
| an abnormal estrual pattern in a goat (a case history). | | 1977 | 242890 |
| thiamylal sodium anesthesia in the goat. | | 1977 | 243338 |
| frozen storage of embryos of farm animals: progress and implications. | | 1977 | 244396 |
| dilatation of the uterine cervix. | dilatation of the cervix at parturition necessitates changes in the physical characteristics of cervical collagen and glycosaminoglycans which are probably hormone-dependent. the infusion of prostaglandin f2alpha (pgf2alpha) into the arterial blood supply to uterus and cervix of sheep pregnant for about 125 days resulted in cervical dilatation after 24 hours but this response was inconsistent; one factor affecting the response appeared to be the progesterone status, low circulating progesterone ... | 1977 | 246393 |
| the fetal role in timing the initiation of parturition in the goat. | | 1977 | 246396 |
| ketamine and xylazine anesthesia in the goat. | | 1978 | 249136 |
| viral leukoencephalomyelitis in two minnesota goats. | | 1978 | 251362 |
| examination of bucks for breeding soundness. | | 1978 | 252347 |
| mastectomy in a goat (a case report). | | 1978 | 252359 |
| hemivertebra in a goat. | | 1979 | 255013 |
| effect of cold exposure on mammary gland uptake of fat precursors and secretion of milk fat and carnitine in the goat. | | 1979 | 256169 |
| viral leukoencephalomyelitis in a goat in michigan. | | 1979 | 256362 |
| a survey of caseous lymphadenitis and its etiology in goats in the united states. | | 1979 | 260549 |
| prevention and control of mastitis in dairy goats. | | 1979 | 260575 |
| serological studies with the virus of peste des petits ruminants in nigeria. | it was possible to distinguish separate serological responses when experimental goats were inoculated with either rinderpest virus or peste des petits ruminants virus. examination of field samples established that peste des petits ruminants occur commonly in nigerian sheep and goats although some villages have escaped recent infection. there was no evidence of infection with rinderpest in these animals. | 1979 | 262608 |
| [the fine structure of hemal nodes in goats, with special reference to the passage of intranodal migration of erythrocytes]. | | 1979 | 262771 |
| [brucellosis in goats in san luis province]. | a study on goat brucellosis has been done in the province of san luis, a semidesertic area (annual precipitation ca. 500 ml) located in the center west of argentina. flocks were selected by an statistical method. blood samples were obtained from young and adult females set apart for reproduction. sera were submitted to a fast plate agglutination test with a standard antigen. titres were expressed in international units per ml, accorded to the recommendations of the centro panamericano de zoonosi ... | 1979 | 263179 |
| path analysis: application in an epidemiological study of echinococcosis in new zealand. | the method of path analysis is described in detail. application of this analyogical systems is demonstrated using data from an epidemiological study of echinococcosis in new zealand. the results identified the major causal pathways determining echinococcus granulosus prevalence in dogs, based on multiple regression analysis of a linear causal model constructed from prior biological and epidemiological knowledge. only ethnic and sheep husbandry variables had important direct effect on prevalence ... | 1977 | 265339 |
| epidemiological analysis of factors influencing rate of progress in echinococcus granulosus control in new zealand. | the factors influencing the rate of progress in echinococcus granulosus control in new zealand were analysed by hydatid control area using stepwise multiple regression techniques. the results indicated that the rate of progress was related positively to initial e. granulosus prevalence in dogs and the efficiency with which local authorities implemented national control policy, and negatively to the maori proportion in the local population and the number of dogs per owner. problems in analysis of ... | 1977 | 265340 |
| influence of cholinergic receptors on cerebral blood flow of the goat. | | 1977 | 268813 |
| antibodies to estrogen receptor: immunochemical similarity of estrophilin from various mammalian species. | immunoglobulin obtained from the serum of rabbits immunized with a highly purified preparation of estradiol-receptor complex from calf uterine nuclei has been shown to contain specific antibodies to the receptor protein (estrophilin) by four criteria: (a) precipitation of the radioactive steroid upon addition of goat antibody against rabbit gamma globulin to a mixture of the tritiated estradiol-receptor complex and the immunoglobulin, (b) adsorption of the estradiol-receptor complex by the immun ... | 1977 | 269423 |
| hemoglobin switching in sheep and goats: induction of hemoglobin c synthesis in cultures of sheep fetal erythroid cells. | synthesis of hbf (alpha2gamma2) is replaced by synthesis of hb a (alpha2betaa2) shortly before birth in sheep homozygous for the betaa globin chain whereas hb c (alpha2betac2) is produced transiently during the neonatal period. we have obtained a hb f-to-hb c switch by generating erythroid colonies at high erythropoietin concentration in plasma clot cultures of mid-gestation fetal bone marrow or liver. furthermore, high erythropoietin concentration appeared specifically to activate the gene for ... | 1977 | 270742 |
| abnormal cellular localization of thyroglobulin mrna associated with hereditary congenital goiter and thyroglobulin deficiency. | the goiters in a breed of hypothyroid goats contain only minute amounts of thyroblobulin-related antigens (0.01% of normal value). we have analyzed these goiters for the presence of mrna coding for thyroglobulin. using dna complementary to beef 33s thyroglobulin mrna as a probe, we found that the mrna sequence is present in the goat goiter but at a concentration 1/10-1/40 that of normal goat thyroid. hybrids of cdna with either goiter or normal thyroid rna exhibited identical sharp melting curve ... | 1978 | 272675 |
| selective complexing of the "nuclear" 5s estradiol receptor by a serum component, 5s-ca. | a component present in several sera interacts selectively with the 5s "nuclear" form of estradiol receptor but not with the 4s "cytosol" form. this component is therefore referred to as "5s-complexing activity (5s-ca)." this selective interaction occurs with 5s receptor from mouse, calf, or rat. in addition, 5s-ca recognizes the nuclear form of the receptor from hypothalamic as well as uterine tissue thus, 5s-ca indicates a general similarity in the chemical nature of 5s nuclear receptors prepar ... | 1978 | 275834 |
| goat milk in infant feeding. | | 1978 | 276718 |
| localization of acetylcholine receptor in excitable membrane from the electric organ of torpedo: evidence for exposure of receptor antigenic sites on both sides of the membrane. | nicotinic acetylcholine receptor was localized in a receptor-rich membrane preparation from the electric organ of torpedo californica by applying an immunoferritin technique. the membrane preparation was incubated with (fab')2 fragments derived from specific rabbit antibodies against the purified acetylcholine receptor and subsequently with ferritin-conjugated goat antiserum to rabbit immunoglobulin. more than 50% of the vesicles were found to be labeled with ferritin while the rest remained unl ... | 1978 | 276886 |
| roux-y intestinal bypass for administration of sorbents in uremia. | in order to minimize interaction of sorbents with food and digestive secretions, an intestinal bypass was created for sorbent administration in normal and uremic rats (n = 18) and goats (n = 5). two separate limbs of small intestine were fashioned, one for food absorption and one for sorbent function, which joined at a roux-y anastomosis before the cecum. particulate sorbent suspensions were injected into the intestine via a cutaneous stoma, and were excreted with food wastes in the feces. in an ... | 1978 | 278884 |
| problems of sheep and goats. | | 1978 | 287103 |
| selected mycoplasmosis of goats in the united states. | | 1978 | 287104 |
| isolation of mycoplasma and ureaplasma from sheep and goats in west texas (1970-78). | | 1978 | 287105 |
| mycoplasmosis in arizona goats. | | 1978 | 287107 |
| isolation of mycoplasma from pneumonic lungs of sheep and goats in mexico. | | 1978 | 287108 |
| complement-dependent cytotoxic antitumor antibody. ii. quantitative determination of cell-bound immunoglobulin m1. | a method for the quantitative determination of rabbit igm bound to cell surfaces has been developed. this method was based on the ability of goat igg specific for rabbit igm heavy chains to bind 125i-labeled protein a when bound to the antigen. with the use of this technique the production of specific igm antitumor antibodies in new zealand white rabbits after immunization with guinea pig hepatoma cells line-1 and line-10 was followed. differences in the production of igm were observed between t ... | 1979 | 286124 |
| muscle membrane chloride conductance and the myotonic syndromes. | the myotonic syndrome of delayed relaxation in striated muscle associated with repetitive membrane electrical activity is characteristic of a number of hereditary disorders in animals and man. this state of pathologic membrane hyperexcitability is also seen as a consequence of intoxication with several classes of chemicals. in most of these states an increase in muscle membrane resistance due to a specific reduction in sarcolemmal gcl can be demonstrated. computer simulations have indicated that ... | 1978 | 285855 |
| aspects on the choice of experimental animals when reproducing missile trauma. | | 1979 | 291246 |
| the histocompatibility complex gla in the goat. | the current status of research on the major histocompatibility complex of the goat (gla) is discussed, as well as its similarities to other species, although some of the results are preliminary. | 1979 | 292353 |
| a veterinary group for goats? | | 1979 | 292904 |
| humoral factor that specifically regulates prothrombin (factor ii) levels (coagulopoietin-ii). | prothrombin (factor ii) was decreased in rabbits by injection of a goat antiserum to prothrombin. when plasma from these rabbits was injected into normal animals, circulating prothrombin increased in the recipients, suggesting transfer of a humoral regulating factor ("coagulopoietin-ii"). this coagulopoietin-ii resisted boiling for 20 min. on sephadex g-50 gel filtration, it remained in the retained volume, suggesting a molecular weight of less than 30,000. in an in vitro liver system, prothromb ... | 1979 | 284365 |
| neuromuscular diseases of domestic animals: a summary of muscle biopsies from 159 cases. | | 1979 | 289312 |
| myotonia in the goat. | | 1979 | 289314 |
| electron microscopic visualization of n-acetoxy-n-2-acetylaminofluorene binding sites in cole1 dna by means of specific antibodies. | cole1 dna has been allowed to react in vitro with n-acetoxy-n-2-[14c]acetylaminofluorene in the range of 0-15 n-2-[14c]acetylaminofluorene residues bound per molecule of dna, at the c8 of guanine residues. purified rabbit antibodies to both n-2-(guanosine-8-yl)-acetylaminofluorene and native dna that had reacted with n-acetoxy-n-2-acetylaminofluorene were shown by electron microscopy to recognize specifically the acetylaminofluorene-modified cole1 dna. the antibodies bound to dna were visualized ... | 1979 | 293703 |
| immunochemical studies of infectious mononucleosis. vi. a radioimmunoassay for the detection of infectious mononucleosis heterophile antibody and antigen. | the coated-tube method of solid-phase radioimmunoassay has been adapted to the detection of heterophile antibodies and antigens of infectious mononucleosis. disposable plastic hemagglutination trays were coated with purified glycoprotein from horse erythrocytes and the subsequent uptake of antibody from test sera was detected by radio-iodinated horse erythrocyte glycoprotein. in a preliminary survey of sera from patients with infectious mononucleosis and sera from controls, the assay proved high ... | 1977 | 299865 |
| a method for radioactive antiglobulin testing with 125i labeled anti-igg. | the present report describes a method for 125i anti-igg antiglobulin testing. the method involves the use of a labeled deae cellulose igg fraction of goat anti-human igg globulin. leukocyte-free red blood cells were used in the test procedure. both the labeled antiserum and the red blood cell suspensions were diluted in normal rabbit serum to prevent non-specific uptake of labeled antiserum by normal, uncoated red blood cells. the results of the study show that the method is highly reproducible ... | 1977 | 300195 |
| relationship of surface immunoglobulin-bearing cells, plasma cells, and tumor development in anaplastic carcinoma-bearing a/j mice. | since the humoral immune response has been shown to be associated with immunological enhancement of tumor growth, the study of surface immunoglobulin-bearing cells and plasma cell antigen (pca)-bearing cells during neoplastic development may provide new approaches to the study of tumor immunology. peripheral blood was collected every other day from normal and carcinoma-bearing mice. lymphocytes obtained by ficoll-hypaque density centrifugation were assayed for immunoglobulin-bearing cells and pc ... | 1977 | 300278 |
| termination of pregnancy in goats using cloprostenol. | | 1979 | 295100 |
| a veterinarian's glimpse at animal health in the people's republic of china. | | 1979 | 298910 |
| immune response of steers, goats and sheep to inactivated rift valley fever vaccine. | | 1979 | 298921 |
| double-antibody radioimmunoassay for factor viii-related antigen. | a plasma protein required for the support of ristocetin-induced platelet aggregation was isolated from antihemophilic factor concentrate and radiolabeled with 125i. a double-antibody radioimmunoassay was developed, with use of specific rabbit anti-viii related antigen serum and goat anti-rabbit globulin. the assay is sensitive, reproducible, and technically simple to perform. values obtained in normal subjects ranged from 0.65 to 1.53 units, similar to our normal range for viii coagulant activit ... | 1977 | 302160 |
| studies on dna synthesis in goat mammary organ culture under the influence of various hormones. | a study has been made of the hormones required for stimulation of dna synthesis in organ culture of mammary tissue of goats. on culture in waymouth's medium without added hormones the incorporation of 6-3h-thymidine into dna was low. the addition of insulin caused a high stimulation of thymidine incorporation. however, the addition of cortisol, prolactin, growth hormone, 17beta-estradiol, progesterone alone or in combination had no effect on dna synthesis. the minimal effective concentration of ... | 1977 | 301818 |
| antisera against leukaemia-associated antigens on human lymphocytes. | antisera were raised in rabbits against leukaemic lymphosarcoma (lsl) cells which carried surface markers of both thymus-derived t lymphocytes (t cells) and bone marrow-derived b lymphocytes (b cells). after absorption with leucocytes, erythrocytes and serum proteins from normal individuals, the antisera demonstrated significant complement-dependent cytotoxicity against leukaemic cells from patients with acute lymphoblastic leukaemia (all) (9/11), lsl (7/9) and chronic lymphocytic leukaemia (cll ... | 1977 | 301073 |
| preparation of a phospholipase c-antihuman igg conjugate, and inhibition of its enzymatic activity by human igg. | we labeled igg with phospholipase c, using 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide. enzymaticactivity of the resulting conjugate was inhibited when it was complexed with human igg, but rabbit or goat igg was not effective in suppressing the enzyme activity. normal erythrocytes were used as substrate for the enzyme, enzymatic activity being assessed by measuring the release of hemoglobin. the substrates for phospholipase c are phospholipids, which are major components of the erythrocyte m ... | 1977 | 301445 |
| inactivation of antigen-responsive clones with antisera specific for igm or igd. | the present study examines the isotype of cell surface immunoglobulins involved in triggering a response to thymus-dependent and thymus-independent antigens. antibodies to immunoglobulin isotypes were used to block the in vitro interaction of receptor igd and igm with antigen. the results indicate that both igd and igm are necessary to trigger a response to tnp-srbc but that only igm is required for responsiveness to tnp-brucella. limiting dilution studies indicate that the inhibition of the imm ... | 1978 | 305934 |
| isolation and characterization of a rabbit t lymphocyte-specific antigen. | rabbit t lymphocytes may be differentiated from b lymphocytes by the presence of a t lymphocyte-specific surface antigen. this unique antigen has been extracted from the plasma membrane of rabbit thymocytes by 3 m kcl. the presence of the antigen in the membrane extract was demonstrated by inhibition of cytotoxicity with goat anti-rabbit t cell serum (ats). the crude membrane extract was fractionated by gel electrophoresis and the fractions containing the t cell antigen identified by inhibition ... | 1978 | 305942 |
| further characterization of the suppressor cells, activated by goat anti-th-b antibody reagent and responsible for enhanced growth of sarcoma 180 in akr mice. | | 1978 | 306887 |
| regulation of delayed-type hypersensitivity. ii. specific suppressor factor for delayed-type hypersensitivity to sheep erythrocytes in mice. | an antigen-specific suppressor factor for delayed-type hypersensitivity (dth) to sheep red blood cells (srbc) in mice is described. lymph node cells and spleen cells from mice injected intravenously with 1 x 10(9) srbc 4 days previously were incubated in vitro for 48 h in culture medium. supernatant obtained from the culture inhibited the induction of dth to srbc in normal mice. it also suppressed the expression of dth in presensitized mice. the suppression is specific as the suppressor factor h ... | 1978 | 306924 |
| a double-antibody radioimmunoassay for serum progesterone using progesterone-3-(o-carboxymethyl) oximino-[125i]-iodo-histamine as radioligand. | a reliable, convenient and economical radioimmunoassay (ria) for serum progesterone has been established and tested. this procedure employs diethyl ether extraction followed by ria utilizing rabbit anti-11 alpha-hydroxyprogesterone 11-hemisuccinyl-bovine serum albumin (progesterone-11 alpha-bsa) serum, progresterone-3-(o-carboxymethyl) oximino-[125i]-iodohistamine (progesterone-3-[125i]) as radioligand and goat anti-rabbit gamma globulin as second antibody. in conjunction with antiprogesterone-1 ... | 1978 | 307289 |
| preparation of anti-human globulin serum in bangladeshi rabbits and goats. | anti-human globulin was produced in local rabbits and goats by using human o serum mixed with freund's adjuvants (complete or incomplete) for immunising the animals. a titre of 1/1024 was obtained in rabbit and a titre of 1/8192 was obtained in goat. quality-and quantity-wise, goat's serum appeared to be better than rabbit's serum. | 1977 | 307952 |
| rosette formation with goat erythrocytes. a marker for human t lymphocytes. | | 1978 | 308424 |
| requirement for antigen in lipopolysaccharide-dependent induction of b cells. | the magnitude of the igm response to a variety of antigens, induced on the in vivo administration of lipopolysaccharide (lps) to mice, is approximately proportional to the magnitude of the background response observed in untreated animals. this striking correlation suggests that the administration of lps alone cannot in general induce a specific response in the absence of a background response. such is the case for the antigen rat erythrocytes against which no lps-dependent or background respons ... | 1978 | 308459 |