Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| improved bleeding scores using gelfoam(®) powder with incremental concentrations of bovine thrombin in a swine liver lesion model. | topical hemostatic agents are used intra-operatively to prevent uncontrolled bleeding. gelfoam(®) powder contains a hemostatic agent prepared from purified pork skin gelatin, the efficacy of which is increased when combined with thrombin. however, the effect of increasing concentrations of thrombin on resultant hemostasis is not known. this study sought to evaluate the ability of various concentrations of thrombin in combination with gelfoam powder to control bleeding using a swine liver lesion ... | 0 | 27334382 |
| an mrna vaccine encoding rabies virus glycoprotein induces protection against lethal infection in mice and correlates of protection in adult and newborn pigs. | rabies is a zoonotic infectious disease of the central nervous system (cns). in unvaccinated or untreated subjects, rabies virus infection causes severe neurological symptoms and is invariably fatal. despite the long-standing existence of effective vaccines, vaccine availability remains insufficient, with high numbers of fatal infections mostly in developing countries. nucleic acid based vaccines have proven convincingly as a new technology for the fast development of vaccines against newly emer ... | 2016 | 27336830 |
| cross-reactivity of porcine immunoglobulin a antibodies with fecal immunoglobulins of wild boar (sus scrofa) and other animal species. | fecal samples obtained from wild boar habitats are useful for the surveillance of diseases in wild boar populations; however, it is difficult to determine the species of origin of feces collected in natural habitats. in this study, a fecal iga elisa was evaluated as a method for identifying the porcine species from fecal samples. both domestic pigs (sus scrofa domestica) and wild boars (sus scrofa coreanus) showed significantly higher levels of fecal iga than other animal species. additionally, ... | 2016 | 27340389 |
| dynamics of myosin heavy chain isoform transition in the longissimus muscle of domestic and wild pigs during growth: a comparative study. | dynamics of myofiber differentiation/maturation in porcine skeletal muscle is associated with domestication, breeding and rearing conditions. this study was aimed to comparatively elucidate the age-dependent myosin heavy chain (myhc) isoform expression and transition pattern in domestic and wild pig (wp) skeletal muscle from birth until adulthood. domestic pigs (dps) of large white breed raised in conventional production system were compared with wps reared in a large hunting enclosure. muscle s ... | 2017 | 27345286 |
| brain mass and encephalization quotients in the domestic industrial pig (sus scrofa). | in the present study we examined the brain of fetal, newborn, and adult pigs raised for meat production. the fresh and formalin-fixed weights of the brain have been recorded and used, together with body weight, to calculate the encephalization quotient (eq). the weight of the cerebellum has been used to calculate the cerebellar quotient (cq). the results have been discussed together with analogue data obtained in other terrestrial cetartiodactyla (including the domestic bovine, sheep, goat, and ... | 2016 | 27351807 |
| evidence of aujeszky's disease in wild boar in serbia. | aujeszky's disease is a viral disease of suids caused by suid herpesvirus 1. the disease has worldwide distribution with significant economic impact. in serbia, there is neither an aujeszky's disease eradication nor national vaccination programme of domestic pigs. since clinical symptoms of aujeszky's disease are not specific, it is important to establish a link between clinical signs and presence of adv active infection in wild boars. the aim of this study was to investigate the possibility of ... | 2016 | 27357597 |
| evolutionary diversity of suid herpesvirus 1 based on ul44 partial sequences. | the aim of this study was to use partial ul44 sequences (glycoprotein c) of suid herpesvirus 1 to examine the evolution and dynamics of the virus in different periods and hosts. | 2016 | 27362774 |
| experimental taphonomy: post-mortem microstructural modifications in sus scrofa domesticus bone. | bone is a highly specialised form of hard and rigid connective tissue whose histological structure undergoes post-mortem modifications. in taphonomic research, histological examination of bone thin sections is used to investigate these post-mortem microstructural changes in skeletal tissues. in this study, diagenetic modifications in pig skeletal remains (sus scrofa domesticus) which were exposed to different taphonomic conditions as part of a long-term, real-time experiment were examined under ... | 2016 | 27368073 |
| using na3po4 to enhance in vitro animal models of aortic valve calcification. | the pathogenesis of calcific aortic valvular disease (cavd) involves an active inflammatory process of valvular interstitial cells (vics) characterized by the activation of specific osteogenic signaling pathways and apoptosis. this process can be studied by analyzing certain molecular markers and gene expression pathways of spontaneous calcification. the purpose of our study is to investigate the role of sodium phosphate (na3po4) as a calcification promoter, with the aim of improving in vitro an ... | 0 | 27376093 |
| identification of bapa in strains of salmonella enterica subsp. enterica isolated from wild animals kept in captivity in sinaloa, mexico. | bapa, previously named stm2689, encodes the bapa protein, which, along with cellulose and fimbriae, constitutes biofilms. biofilms are communities of microorganisms that grow in a matrix of exopolysaccharides and may adhere to living tissues or inert surfaces. biofilm formation is associated with the ability to persist in different environments, which contributes to the pathogenicity of several species. we analyzed the presence of bapa in 83 strains belonging to 17 serovars of salmonella enteric ... | 2016 | 27379195 |
| partially protective immunity induced by the 14-3-3 protein from trichinella spiralis. | trichinellosis, a widespread zoonosis, is considered to be an emerging or re-emerging infectious parasitic disease. the development of vaccines to prevent trichinella infection in domestic animals and humans is important for disease control. in a previous study, we identified ts14-3-3 as an immunodominant protein from trichinella spiralis (t. spiralis) adult worms recognized by early infection sera from pigs and mice. in this study, we further confirmed that ts14-3-3 mrna is expressed in both ad ... | 2016 | 27387374 |
| coating of intravascular balloon with paclitaxel prevents constrictive remodeling of the dilated porcine femoral artery due to inhibition of intimal and media fibrosis. | here we investigated how a coating of intravascular balloon with paclitaxel (drug-coated balloon; dcb, freeway™) impacted porcine peripheral artery vascular function and remodeling. domestic swine (n = 54) underwent percutaneous overstretch balloon dilation of femoral and iliac arteries, controlled by angiography and optical coherence tomography (oct). paclitaxel tissue uptake was measured at 1 h and 1, 3, and 9 days post-dilation. at these time-points and at 32 ± 2 days, vascular function of th ... | 2016 | 27388164 |
| recombinant protein p30 for serological diagnosis of african swine fever by immunoblotting assay. | this article is devoted to the development and evaluation of the immunoblotting test system for serological diagnosis of african swine fever (asf), based on the highly purified recombinant p30 of asf virus (asfv) strain stavropol 01/08 (stavropol 2008), representative of the asfv currently circulating in the russian federation. the main project stages are as follows: (i) cloning of the central hydrophilic region of the asfv gene cp204l (p30) into a prokaryotic vector; (ii) expression and chromat ... | 2016 | 27390151 |
| histopathological comparative analysis of periimplant bone inflammatory response after dental implant insertion using flap and flapless surgical technique. an experimental study in pigs. | to evaluate by histopathological analysis the peri-implant bone inflammation degree, in certain time intervals (7, 14, 21 and 28 days), following mini-incision flapless and flap implant placement. | 2017 | 27393033 |
| rapid detection and subtyping of european swine influenza viruses in porcine clinical samples by haemagglutinin- and neuraminidase-specific tetra- and triplex real-time rt-pcrs. | a diversifying pool of mammalian-adapted influenza a viruses (iav) with largely unknown zoonotic potential is maintained in domestic swine populations worldwide. the most recent human influenza pandemic in 2009 was caused by a virus with genes originating from iav isolated from swine. swine influenza viruses (siv) are widespread in european domestic pig populations and evolve dynamically. knowledge regarding occurrence, spread and evolution of potentially zoonotic siv in europe is poorly underst ... | 2016 | 27397600 |
| reconstructing jaw defects with mscs and plga-encapsulated growth factors. | cell and growth factor-based tissue engineering has shown great potentials for skeletal regeneration. this study tested its feasibility in reconstructing large mandibular defects and compared the efficacy of varied construction materials and sealing methods. bilateral mandibular critical-size (5-cm(3)) defects were created on six 4-month-old domestic pigs, and grafted with β-tricalcium phosphate (βtcp) only (group-a), βtcp with autologous bone marrow-derived mesenchymal stem cells (bm-mscs) (gro ... | 2016 | 27398152 |
| extracorporeal membrane oxygenation improves survival in a novel 24-hour pig model of severe acute respiratory distress syndrome. | extracorporeal membrane oxygenation (ecmo) is increasingly being used to treat severe acute respiratory distress syndrome (ards). however, there is limited clinical evidence about how to optimize the technique. experimental research can provide an alternative to fill the actual knowledge gap. the purpose of the present study was to develop and validate an animal model of acute lung injury (ali) which resembled severe ards, and which could be successfully supported with ecmo. eighteen pigs were r ... | 2016 | 27398166 |
| development of a duplex lateral flow assay for simultaneous detection of antibodies against african and classical swine fever viruses. | classical swine fever (csf) and african swine fever (asf) are both highly contagious diseases of domestic pigs and wild boar and are clinically indistinguishable. for both diseases, antibody detection is an integral and crucial part of prevention and control measures. the purpose of our study was to develop and initially validate a duplex pen-side test for simultaneous detection and differentiation of specific antibodies against csf virus (csfv) and asf virus (asfv). the test was based on the ma ... | 2016 | 27400954 |
| experimental challenge of atlantic cod (gadus morhua) with a brucella pinnipedialis strain from hooded seal (cystophora cristata). | pathology has not been observed in true seals infected with brucella pinnipedialis. a lack of intracellular survival and multiplication of b. pinnipedialis in hooded seal (cystophora cristata) macrophages in vitro indicates a lack of chronic infection in hooded seals. both epidemiology and bacteriological patterns in the hooded seal point to a transient infection of environmental origin, possibly through the food chain. to analyse the potential role of fish in the transmission of b. pinnipediali ... | 2016 | 27415626 |
| feral swine virome is dominated by single-stranded dna viruses and contains a novel orthopneumovirus which circulates both in feral and domestic swine. | feral swine are known reservoirs for various pathogens that can adversely affect domestic animals. to assess the viral ecology of feral swine in the usa, metagenomic sequencing was performed on 100 pooled nasal swabs. the virome was dominated by small, ssdna viruses belonging to the families circoviridae, anelloviridae and parvovirinae. only four rna viruses were identified: porcine kobuvirus, porcine sapelovirus, atypical porcine pestivirus and a novel orthopneumovirus, provisionally named swin ... | 2016 | 27417702 |
| romanian wild boars and mangalitza pigs have a european ancestry and harbour genetic signatures compatible with past population bottlenecks. | we aimed to analyse the genetic diversity of romanian wild boars and to compare it with that from other wild boar and pig populations from europe and asia. partial sequencing of the mitochondrial encoded cytochrome b (mt-cyb) gene from 36 romanian wild boars and 36 domestic pigs (mangalitza, bazna and vietnamese breeds) showed that the diversity of romanian wild boars and mangalitza pigs is fairly reduced, and that most of the members of these two populations share a common mt-cyb haplotype. bes ... | 2016 | 27418428 |
| allometric scaling of the elevation of maternal energy intake during lactation. | in most mammals, lactating mothers dramatically increase their food intake after parturition and reach a peak intake rate after a certain time while their offspring continue to grow. a common view, perpetuated by the metabolic theory of ecology, is that the allometric scaling of maternal metabolic rate with body mass limits the changes in energy intake and expenditure. therefore these potential effects of metabolic scaling should be reflected in the elevation of maternal energy intake during lac ... | 2016 | 27418939 |
| application of a handheld pressure application measurement device for the characterisation of mechanical nociceptive thresholds in intact pig tails. | the assessment of nociceptive thresholds is employed in animals and humans to evaluate changes in sensitivity potentially arising from tissue damage. its application on the intact pig tail might represent a suitable method to assess changes in nociceptive thresholds arising from tail injury, such as tail docking or tail biting. the pressure application measurement (pam) device is used here for the first time on the tail of pigs to determine the reliability of the methods and to provide novel dat ... | 2016 | 27422675 |
| acoustic signalling reflects personality in a social mammal. | social interactions among individuals are often mediated through acoustic signals. if acoustic signals are consistent and related to an individual's personality, these consistent individual differences in signalling may be an important driver in social interactions. however, few studies in non-human mammals have investigated the relationship between acoustic signalling and personality. here we show that acoustic signalling rate is repeatable and strongly related to personality in a highly social ... | 2016 | 27429775 |
| possible foodborne transmission of hepatitis e virus from domestic pigs and wild boars from corsica. | 2016 | 27437570 | |
| a naturally hypersensitive glucocorticoid receptor elicits a compensatory reduction of hypothalamus-pituitary-adrenal axis activity early in ontogeny. | we comprehensively characterized the effects of a unique natural gain-of-function mutation in the glucocorticoid receptor (gr), grala610val, in domestic pigs to expand current knowledge of the phenotypic consequences of gr hypersensitivity. cortisol levels were consistently reduced in one-week-old piglets, at weaning and in peripubertal age, probably due to a reduced adrenal capacity to produce glucocorticoids (gc), which was indicated by an adrenocortical thinning in grala610val carriers. adren ... | 2016 | 27440422 |
| establishing the volatile profile of pig carcasses as analogues for human decomposition during the early postmortem period. | following a mass disaster, it is important that victims are rapidly located as the chances of survival decrease greatly after approximately 48 h. urban search and rescue (usar) teams may use a range of tools to assist their efforts but detector dogs still remain one of the most effective search tools to locate victims of mass disasters. usar teams can choose to deploy human scent dogs (trained to locate living victims) or human remains detection (hrd) dogs (trained to locate deceased victims). h ... | 2016 | 27441249 |
| denosumab could be a potential inhibitor of valvular interstitial cells calcification in vitro. | denosumab is a fully human monoclonal antibody and novel antiresorptive agent that works by binding receptor activator of nuclear factor kappa-β ligand (rankl) and inhibiting the signaling cascade that causes osteoclast maturation, activity, and survival. we aimed to elucidate the effect of denosumab in the process of spontaneous and induced calcification in an in vitro porcine valvular interstitial cells (vics) model. | 0 | 27468412 |
| the challenge of detecting classical swine fever virus circulation in wild boar (sus scrofa): simulation of sampling options. | classical swine fever (csf) is one of the most important viral diseases of domestic pigs ( sus scrofa domesticus) and wild boar ( sus scrofa ). for at least 4 decades, several european union member states were confronted with outbreaks among wild boar and, as it had been shown that infected wild boar populations can be a major cause of primary outbreaks in domestic pigs, strict control measures for both species were implemented. to guarantee early detection and to demonstrate freedom from diseas ... | 2016 | 27479901 |
| prevalence of antibody to and dna of lawsonia intracellularis in samples from wild animals in korea. | we evaluated the prevalence of lawsonia intracellularis infection in three wild animal species in korea; the korean water deer ( hydropotes inermis ), siberian roe deer ( capreolus pygargus ), and raccoon dogs ( nyctereutes procyonoides ). we collected 136 sera and 109 fecal samples from individuals in 10 wildlife rescue and conservation centers. serum samples were tested for anti- l. intracellularis antibodies using a blocking enzyme-linked immunosorbent assay (belisa), and fecal samples were s ... | 2016 | 27487235 |
| technical note: evaluation of a 160-mm/256-row ct scanner for whole-heart quantitative myocardial perfusion imaging. | the authors investigated the performance of a recently introduced 160-mm/256-row ct system for low dose quantitative myocardial perfusion (mp) imaging of the whole heart. this platform is equipped with a gantry capable of rotating at 280 ms per full cycle, a second generation of adaptive statistical iterative reconstruction (asir-v) to correct for image noise arising from low tube voltage potential/tube current dynamic scanning, and image reconstruction algorithms to tackle beam-hardening, cone- ... | 2016 | 27487900 |
| an oral aujeszky's disease vaccine (ys-400) induces neutralizing antibody in pigs. | aujeszky's disease (ad) is an economically important disease affecting both wild and domestic pigs of the species sus scrofa. a previous study yielded serological evidence of ad in korean wild boars, which could spread ad to other animals. a new aujeszky's disease virus (adv) bait vaccine is required to prevent ad outbreaks in swine. in the present study, we investigated the safety and immunogenicity of a ge-deleted marker vaccine, strain ys-400, in young domestic pigs. | 2016 | 27489803 |
| a recombinant rabies virus (erags) for use in a bait vaccine for swine. | rabies viruses (rabv) circulating worldwide in various carnivores occasionally cause fatal encephalitis in swine. in this study, the safety and immunogenicity of a recombinant rabies virus, the erags strain constructed with a reverse genetics system, was evaluated in domestic pigs. | 2016 | 27489807 |
| cholinergic profiles in the goettingen miniature pig (sus scrofa domesticus) brain. | central cholinergic structures within the brain of the even-toed hoofed goettingen miniature domestic pig (sus scrofa domesticus) were evaluated by immunohistochemical visualization of choline acetyltransferase (chat) and the low-affinity neurotrophin receptor, p75ntr. chat-immunoreactive (-ir) perikarya were seen in the olfactory tubercle, striatum, medial septal nucleus, vertical and horizontal limbs of the diagonal band of broca, and the nucleus basalis of meynert, medial habenular nucleus, z ... | 2017 | 27490949 |
| force per cross-sectional area from molecules to muscles: a general property of biological motors. | we propose to formally extend the notion of specific tension, i.e. force per cross-sectional area-classically used for muscles, to quantify forces in molecular motors exerting various biological functions. in doing so, we review and compare the maximum tensions exerted by about 265 biological motors operated by about 150 species of different taxonomic groups. the motors considered range from single molecules and motile appendages of microorganisms to whole muscles of large animals. we show that ... | 2016 | 27493785 |
| onchocerciasis transmission in ghana: the human blood index of sibling species of the simulium damnosum complex. | vector-biting behaviour is important for vector-borne disease (vbd) epidemiology. the proportion of blood meals taken on humans (the human blood index, hbi), is a component of the biting rate per vector on humans in vbd transmission models. humans are the definitive host of onchocerca volvulus, but the simuliid vectors feed on a range of animals and hbi is a key indicator of the potential for human onchocerciasis transmission. ghana has a diversity of simulium damnosum complex members, which are ... | 2016 | 27494934 |
| the ep152r orf of african swine fever virus strain georgia encodes for an essential gene that interacts with host protein bag6. | african swine fever virus (asfv) is the etiological agent of a contagious and often lethal disease of domestic pigs that has significant economic consequences for the swine industry. the viral genome encodes for more than 150 genes, and only a select few of these genes have been studied in some detail. here we report the characterization of open reading frame ep152r that has a predicted complement control module/scr domain. this domain is found in vaccinia virus proteins that are involved in blo ... | 2016 | 27497620 |
| dynamic variables fail to predict fluid responsiveness in an animal model with pericardial effusion. | the reliability of dynamic and volumetric variables of fluid responsiveness in the presence of pericardial effusion is still elusive. the aim of the present study was to investigate their predictive power in a porcine model with hemodynamic relevant pericardial effusion. | 2016 | 27499343 |
| foraging choices of vampire bats in diverse landscapes: potential implications for land-use change and disease transmission. | in latin america, the common vampire bat desmodus rotundus is the primary reservoir of rabies, a zoonotic virus that kills thousands of livestock annually and causes sporadic and lethal human rabies outbreaks. the proliferation of livestock provides an abundant food resource for this obligate blood-feeding species that could alter its foraging behaviour and rabies transmission, but poor understanding of the dietary plasticity of vampire bats limits understanding of how livestock influences rabie ... | 2016 | 27499553 |
| an experimental comparative study on classic tube thoracostomy and thoracostomy with a newly designed thorax drainage catheter. | the collection of fluids, blood, pus, or air in the pleural cavity is a pathological condition requiring pleural drainage. a newly designed thorax drainage catheter in the prototype phase was used in this experimental study to test its efficacy. | 2016 | 27511340 |
| antigenic characterization of classical swine fever virus yc11wb isolates from wild boar. | classical swine fever (csf), a highly contagious disease that affects domestic pigs and wild boar, has serious economic implications. the present study examined the virulence and transmission of strain yc11wb (isolated from a wild boar in 2011) in breeding wild boar. virulence in domestic pigs was also examined. based on the severe clinical signs and high mortality observed among breeding wild boar, the pathogenicity of strain yc11wb resembled that of typical acute csf. surprisingly, in contrast ... | 2016 | 27515269 |
| evaluation of a microfluidic chip system for preparation of bacterial dna from swabs, air, and surface water samples. | the detection of bacterial pathogens from complex sample matrices by pcr requires efficient dna extraction. in this study, a protocol for extraction and purification of dna from swabs, air, and water samples using a microfluidic chip system was established. the optimized protocol includes a combination of thermal, chemical and enzymatic lysis followed by chip-based dna purification using magnetic particles. the procedure was tested using gram-positive bacillus thuringiensis berliner var. kurstak ... | 2016 | 27520284 |
| deletion of african swine fever virus interferon inhibitors from the genome of a virulent isolate reduces virulence in domestic pigs and induces a protective response. | african swine fever virus (asfv) encodes multiple copies of mgf360 and mgf530/505 gene families. these genes have been implicated in the modulation of the type i interferon (ifn) response. we investigated the effect of modulating the ifn response on virus attenuation and induction of protective immunity by deleting genes mgf360 (mgf360-10l, 11l, 12l, 13l, 14l) and mgf530/505 (mgf530/505-1r, 2r and 3r) and interrupting genes (mgf360-9l and mgf530/505-4r) in the genome of the virulent asfv isolate ... | 2016 | 27521231 |
| companion animals as a source of viruses for human beings and food production animals. | companion animals comprise a wide variety of species, including dogs, cats, horses, ferrets, guinea pigs, reptiles, birds and ornamental fish, as well as food production animal species, such as domestic pigs, kept as companion animals. despite their prominent place in human society, little is known about the role of companion animals as sources of viruses for people and food production animals. therefore, we reviewed the literature for accounts of infections of companion animals by zoonotic viru ... | 2016 | 27522300 |
| porcine circovirus 2 (pcv2) increases the expression of endothelial adhesion/junction molecules. | porcine circovirus type 2 (pcv2) is the primary causative agent of porcine circovirus disease, a complex multisystem syndrome in domestic pigs. despite the significant economic losses caused by porcine circovirus disease, the mechanisms of pathogenesis underlying the clinical findings remain largely unclear. as various reports have highlighted the potential key role of vascular lesions in the pathogenesis of porcine circovirus disease, the aim of this work was to investigate effects of pcv2 infe ... | 2016 | 27522934 |
| whole-genome mapping reveals a large chromosomal inversion on iberian brucella suis biovar 2 strains. | optical mapping is a technology able to quickly generate high resolution ordered whole-genome restriction maps of bacteria, being a proven approach to search for diversity among bacterial isolates. in this work, optical whole-genome maps were used to compare closely-related brucella suis biovar 2 strains. this biovar is the unique isolated in domestic pigs and wild boars in portugal and spain and most of the strains share specific molecular characteristics establishing an iberian clonal lineage ... | 2016 | 27527786 |
| periconceptional undernutrition affects in utero methyltransferase expression and steroid hormone concentrations in uterine flushings and blood plasma during the peri-implantation period in domestic pigs. | female undernutrition during early pregnancy may affect the physiological pattern of genomic dna methylation. we hypothesised that in utero dna methylation may be impaired in females fed a restrictive diet in early pregnancy. in this study we evaluated whether poor maternal nutritional status, induced by applying a restricted diet during the peri-conceptional period, may influence: (1) the potential for in utero dna methylation, expressed as changes in the mrna expression and protein abundance o ... | 2017 | 27534526 |
| influence of on-farm pig salmonella status on salmonella shedding at slaughter. | the risk of salmonella shedding among pigs at slaughter with regard to their previous on-farm salmonella status was assessed in a group of pigs from a farm from ne of spain. a total of 202 pigs that had been serologically monitored monthly during the fattening period and from which mesenteric lymph nodes (mln) and faecal (sfec) samples were collected at slaughter for salmonella isolation were included. a repeated-measures anova was used to assess the relationship between mean od% values during t ... | 2017 | 27549508 |
| assessment of domestic pigs, wild boars and feral hybrid pigs as reservoirs of hepatitis e virus in corsica, france. | in corsica, extensive pig breeding systems allow frequent interactions between wild boars and domestic pigs, which are suspected to act as reservoirs of several zoonotic diseases including hepatitis e virus (hev). in this context, 370 sera and 166 liver samples were collected from phenotypically characterized as pure or hybrid wild boars, between 2009 and 2012. in addition, serum and liver from 208 domestic pigs belonging to 30 farms were collected at the abattoir during the end of 2013. anti-he ... | 2016 | 27556478 |
| multispecies purification of testicular germ cells. | advanced methods of cellular purification are required to apply genome technology to the study of spermatogenesis. one approach, based on flow cytometry of murine testicular cells stained with hoechst-33342 (ho-facs), has been extensively optimized and currently allows the isolation of 9 germ cell types. this staining technique is straightforward to implement, highly effective at purifying specific germ cell types and yields sufficient cell numbers for high throughput studies. ho-facs is a techn ... | 2016 | 27557646 |
| novel y-chromosome short tandem repeats in sus scrofa and their variation in european wild boar and domestic pig populations. | y-chromosome markers are important tools for studying male-specific gene flow within and between populations, hybridization patterns and kinship. however, their use in non-human mammals is often hampered by the lack of y-specific polymorphic markers. we identified new male-specific short tandem repeats (strs) in sus scrofa using the available genome sequence. we selected four polymorphic loci (5-10 alleles per locus), falling in one duplicated and two single-copy regions. a total of 32 haplotype ... | 2016 | 27558303 |
| mechanically stable intraspinal microstimulation implants for human translation. | the goal of this study was to develop stable intraspinal microstimulation (isms) implants for use in humans to restore standing and walking after spinal cord injury. isms electrically activates locomotor networks within the lumbar region of the spinal cord. in animals, isms produced better functional outcomes than those obtained by other interventions, and recent efforts have focused on translating this approach to humans. this study used domestic pigs to: (1) quantify the movements and length c ... | 2017 | 27562143 |
| development of primer pairs from molecular typing of rabies virus variants present in mexico. | nucleoprotein (n) gene from rabies virus (rabv) is a useful sequence target for variant studies. several specific rabv variants have been characterized in different mammalian hosts such as skunk, dog, and bats by using anti-nucleocapsid monoclonal antibodies (mabs) via indirect fluorescent antibody (ifa) test, a technique not available in many laboratories in mexico. in the present study, a total of 158 sequences of n gene from rabv were used to design eight pairs of primers (four external and f ... | 2016 | 27563666 |
| urinary bladder matrix scaffolds strengthen esophageal hiatus repair. | laparoscopic repair of the hiatal hernia is associated with a recurrence rate between 12% and 42% depending on the defect size. although the impact of hiatal reinforcement on long-term recurrence remains controversial, the main limitation of this approach has been the risk of adverse events related with the use of synthetic materials in the vicinity of the esophagus. | 2016 | 27565070 |
| in vitro inhibition of african swine fever virus-topoisomerase ii disrupts viral replication. | african swine fever virus (asfv) is the etiological agent of a highly-contagious and fatal disease of domestic pigs, leading to serious socio-economic impact in affected countries. to date, neither a vaccine nor a selective anti-viral drug are available for prevention or treatment of african swine fever (asf), emphasizing the need for more detailed studies at the role of asfv proteins involved in viral dna replication and transcription. notably, asfv encodes for a functional type ii topoisomeras ... | 2016 | 27568922 |
| cerebral autoregulation real-time monitoring. | cerebral autoregulation is a mechanism which maintains constant cerebral blood flow (cbf) despite changes in mean arterial pressure (map). assessing whether this mechanism is intact or impaired and determining its boundaries is important in many clinical settings, where primary or secondary injuries to the brain may occur. herein we describe the development of a new ultrasound tagged near infra red light monitor which tracks cbf trends, in parallel, it continuously measures blood pressure and co ... | 2016 | 27571474 |
| molecular, serological and epidemiological observations after a suspected outbreak of plague in nyimba, eastern zambia. | plague is a re-emerging zoonotic disease caused by the bacterium yersinia pestis. the disease has caused periodic global devastation since the first outbreak in the 6th century. two months after a suspected plague outbreak in nyimba district, samples were collected from 94 livestock (goats and pigs), 25 rodents, 6 shrews and 33 fleas. enzyme-linked immunosorbent assay (elisa) and polymerase chain reaction (pcr) techniques were used to investigate the presence of y. pestis, which showed that 16.0 ... | 2017 | 27578859 |
| first identification of porcine parvovirus 6 in poland. | porcine parvovirus type 1 is a major causative agent of swine reproductive failure. during the past decade, several new parvoviruses have been discovered in pigs. porcine parvovirus type 6 (ppv6), recently identified, has been reported in pigs in china and in the usa while the ppv6 status in the european pig population remains undetermined. in the present study, ppv6 dna was identified in serum samples collected from domestic pigs in poland. in investigated herds, the prevalence of ppv6 was 14.9 ... | 2017 | 27590228 |
| copy number variation contributes to cryptic genetic variation in outbreak lineages of cryptococcus gattii from the north american pacific northwest. | copy number variants (cnvs) are a class of structural variants (svs) and are defined as fragments of dna that are present at variable copy number in comparison with a reference genome. recent advances in bioinformatics methodologies and sequencing technologies have enabled the high-resolution quantification of genome-wide cnvs. in pathogenic fungi svs have been shown to alter gene expression, influence host specificity, and drive fungicide resistance, but little attention has focused specificall ... | 2016 | 27590805 |
| feasibility of three-dimensional placement of human therapeutic stem cells using the intracerebral microinjection instrument. | the ability to safely place viable intracerebral grafts of human-derived therapeutic stem cells in three-dimensional (3d) space was assessed in a porcine model of human stereotactic surgery using the intracerebral microinjection instrument (imi) compared to a conventional straight cannula. | 2016 | 27593216 |
| a cartographic tool for managing african swine fever in eurasia: mapping wild boar distribution based on the quality of available habitats. | the current african swine fever (asf) epidemic in eurasia represents a risk for the swine industry with devastating socio-economic and political consequences. wild boar appears to be a key factor in maintaining the disease in endemic areas (mainly the russian federation) and spreading the disease across borders, including within the european union. to help predict and interpret the dynamics of asf infection, we developed a standardized distribution map based on global land cover vegetation (glob ... | 2017 | 27596983 |
| simulating the epidemiological and economic effects of an african swine fever epidemic in industrialized swine populations. | african swine fever (asf) is a notifiable infectious disease with a considerable impact on animal health and is currently one of the most important emerging diseases of domestic pigs. asf was introduced into georgia in 2007 and subsequently spread to the russian federation and several eastern european countries. consequently, there is a non-negligible risk of asf spread towards western europe. therefore it is important to develop tools to improve our understanding of the spread and control of as ... | 2016 | 27599924 |
| survey of trichinella infection from domestic pigs in the historical endemic areas of henan province, central china. | the aim of this work was to investigate the current situation of trichinella infection from domestic pigs in the historical endemic areas of henan province, central china. a total of 823 diaphragm samples from the indoor-raised pigs were collected in five cities of henan during 2014-2015 and examined by artificial digestion method. the overall prevalence of trichinella infection in pigs was 0.61 % (5/823). trichinella larvae were detected in 0.91 % (5/550) of pigs from nanyang city of henan. the ... | 2016 | 27601238 |
| alteration of a second putative fusion peptide of structural glycoprotein e2 of classical swine fever virus alters virus replication and virulence in swine. | e2, the major envelope glycoprotein of classical swine fever virus (csfv), is involved in several critical virus functions, including cell attachment, host range susceptibility, and virulence in natural hosts. functional structural analysis of e2 based on a wimley-white interfacial hydrophobicity distribution predicted the involvement of a loop (residues 864 to 881) stabilized by a disulfide bond ((869)ckwggnwtcv(878), named fpii) in establishing interactions with the host cell membrane. this lo ... | 2016 | 27605674 |
| detection and molecular characterization of porcine reproductive and respiratory syndrome virus in lithuanian wild boar populations. | porcine reproductive and respiratory syndrome virus (prrsv) is recognized worldwide as an important and economically devastating pathogen in pig production. although prrsv is widespread in domestic swine, there is a lack of information regarding prrsv infection in european wild boars (sus scrofa). currently available information does not provide conclusive evidence that wild boars are a reservoir of prrsv. nevertheless, wild boars may be likely to become infected by domestic swine through occasi ... | 2016 | 27608974 |
| hepatitis e: discovery, global impact, control and cure. | hepatitis e was identified as an epidemic of non-a, non-b hepatitis from kashmir, india in 1978. hepatitis e virus (hev), the etiological agent is the sole member of family hepeviridae. the virus has marked heterogeneity and infects many animals like bats, camel, chicken, deer, boar, mongoose, pigs, rats, rabbit and cutthroat trout. hepatitis e is a disease with a major global impact and has two distinct epidemiological patterns. hepatitis e is an imperative health issue in developing nations, t ... | 2016 | 27610014 |
| a simulation model to determine sensitivity and timeliness of surveillance strategies. | animal surveillance systems need regular evaluation. we developed an easily applicable simulation model of the german wild boar population to investigate two evaluation attributes: the sensitivity and timeliness (i.e. the ability to detect a disease outbreak rapidly) of a surveillance system. classical swine fever (csf) was used as an example for the model. csf is an infectious disease that may lead to massive economic losses. it can affect wild boar as well as domestic pigs, and csf outbreaks i ... | 2017 | 27619421 |
| prevalence and consequences of chromosomal abnormalities in canadian commercial swine herds. | structural chromosome abnormalities are well known as factors that reduce fertility rate in domestic pigs. according to large-scale national cytogenetic screening programs that are implemented in france, it is estimated that new chromosome abnormalities occur at a rate of 0.5 % in fertility-unproven boars. | 2016 | 27620715 |
| the impact of proteolytic pork hydrolysate on microbial, flavor and free amino acids compounds of yogurt. | the aim of this study was to investigate the influence of proteolytic pork hydrolysate (pph) on yoghurt production by lactobacillus delbrueckii subsp. bulgaricus and streptococcus thermophilus. fresh lean pork was cut into pieces and mixed with deionized water and dealt with protease, then the resulting pph was added to milk to investigate the effects of pph on yoghurt production. the fermentation time, the viable cell counts, the flavor, free amino acids compounds, and sensory evaluation of yog ... | 2016 | 27621698 |
| prioritization of zoonotic viral diseases in feral pigs, domestic pigs and humans interface. | understanding the ecology of diseases requires the comprehension of pathogens in wild life-livestock interface. feral pigs (sus scrofa) are a health problem when countries work to prevent and control zoonotic diseases, as their populations raise environmental and health concerns due to infectious agents transmissible to domestic pigs and other animal species, including humans. | 2015 | 27622793 |
| induction of robust immune responses in swine by using a cocktail of adenovirus-vectored african swine fever virus antigens. | the african swine fever virus (asfv) causes a fatal hemorrhagic disease in domestic swine, and at present no treatment or vaccine is available. natural and gene-deleted, live attenuated strains protect against closely related virulent strains; however, they are yet to be deployed and evaluated in the field to rule out chronic persistence and a potential for reversion to virulence. previous studies suggest that antibodies play a role in protection, but induction of cytotoxic t lymphocytes (ctls) ... | 2016 | 27628166 |
| simulation of robotic radical hysterectomy using the porcine model. | this paper describes simulation of robotically-assisted radical hysterectomy using the porcine model. a technique for robotically-assisted radical hysterectomy was developed using 3 domestic pigs. an edited videoclip of the model was assessed by 3 gynecologic oncologists. the steps of the operation are described in detail and shown in a videoclip. overall the procedure simulated that done in the human, both anatomically and surgically. surgical plane development and ureterolysis appears to be ea ... | 2012 | 27638278 |
| apigenin inhibits african swine fever virus infection in vitro. | african swine fever virus (asfv) is one of the most devastating diseases of domestic pigs for which no effective vaccines are available. flavonoids, natural products isolated from plants, have been reported to have significant in vitro and in vivo antiviral activity against different viruses. here, we tested the antiviral effect of five flavonoids on the replication of asfv in vero cells. our results showed a potent, dose-dependent anti-asfv effect of apigenin in vitro. time-of-addition experime ... | 2016 | 27638776 |
| cardioprotective effects of intracoronary administration of 4-chlorodiazepam in small and large animal models of ischemia-reperfusion. | the translocator protein (tspo) of the mitochondrial membrane has been recognized as a potential therapeutic target for mitigation of myocardial ischemia-reperfusion injury. administration of 4-chlorodiazepam (4-cld), a tspo ligand, has been shown to confer acute cardioprotective effects in small animals; however, long-term studies and studies in clinically-relevant large animal models are lacking. in the present study we investigated a potential cardioprotective effect of intracoronary administ ... | 2016 | 27643472 |
| the 'heritability' of domestication and its functional partitioning in the pig. | we propose to estimate the proportion of variance explained by regression on genome-wide markers (or genomic heritability) when wild/domestic status is considered the phenotype of interest. this approach differs from the standard fst in that it can accommodate genetic similarity between individuals in a general form. we apply this strategy to complete genome data from 47 wild and domestic pigs from asia and europe. when we partitioned the total genomic variance into components associated to subs ... | 2017 | 27649617 |
| like a pig out of water: seaborne spread of domestic pigs in southern italy and sardinia during the bronze and iron ages. | southern italy has a long history of human occupation and passage of different cultures since the early holocene. repeated, ancient introductions of pigs in several geographic areas in europe make it difficult to understand pig translocation and domestication in italy. the archeozoological record may provide fundamental information on this, hence shedding light on peopling and on trading among different ancient cultures in the mediterranean. yet, because of the scanty nature of the fossil record ... | 2017 | 27649620 |
| report of mecc-carrying mrsa in domestic swine. | we unexpectedly identified mrsa isolates carrying mecc (mecc-mrsa) from a danish swine farm located in eastern zealand. the objective of the present study was to investigate the origin of these isolates and their genetic relatedness to other mecc-mrsa isolates from zealand. | 2017 | 27650187 |
| the effect of long-term amiodarone administration on myocardial fibrosis and evolution of left ventricular remodeling in a porcine model of ischemic cardiomyopathy. | amiodarone is effective in suppressing arrhythmias in heart failure patients. we investigated the effect of long-term amiodarone administration on myocardial fibrosis and left ventricular (lv) remodeling in a porcine model of ischemic cardiomyopathy. eighteen infarcted farm pigs were randomized to receive long-term amiodarone administration for 3 months (n = 9) or conventional follow-up (n = 9). evolution of lv remodeling over 3 months post-myocardial infarction was examined at tissue level (myo ... | 2016 | 27652141 |
| comparison of microchip transponder and noncontact infrared thermometry with rectal thermometry in domestic swine (sus scrofa domestica). | during disease outbreaks, core temperature is a useful health metric in swine, due to the presence of pyrexia especially during the acute phase of infection. despite technologic advances in other facets of swine production and health management, rectal thermometry continues to be the 'gold standard' for measuring core body temperature. however, for various reasons, collecting rectal temperatures can be difficult and unsafe depending on the housing modality. in addition, the delay between inserti ... | 2016 | 27657715 |
| pharmacokinetics of heroin and its metabolites in vitreous humor and blood in a living pig model. | vitreous humor (vh) is an alternative matrix for drug analysis in forensic toxicology. however, little is known about the distribution of xenobiotics, such as opioids, into vh in living organisms. the aim of this study was to simultaneously measure heroin and metabolite concentrations in blood and vh after injection of heroin in a living pig model. six pigs were under non-opioid anesthesia during the surgical operation and experiment. ocular microdialysis was used to acquire dialysate from vh, a ... | 2016 | 27660664 |
| divergent sapovirus strains and infection prevalence in wild carnivores in the serengeti ecosystem: a long-term study. | the genus sapovirus, in the family caliciviridae, includes enteric viruses of humans and domestic animals. information on sapovirus infection of wildlife is limited and is currently lacking for any free-ranging wildlife species in africa. by screening a large number of predominantly fecal samples (n = 631) obtained from five carnivore species in the serengeti ecosystem, east africa, sapovirus rna was detected in the spotted hyena (crocuta crocuta, family hyaenidae), african lion (panthera leo, f ... | 2016 | 27661997 |
| hyperpolarized13c,15n2-urea mri for assessment of the urea gradient in the porcine kidney. | a decline in cortico-medullary osmolality gradient of the kidney may serve as an early indicator of pathological disruption of the tubular reabsorption process. the purpose of this study was to investigate the feasibility of hyperpolarized13c,15n2-urea mri as a biomarker of renal function in healthy porcine kidneys resembling the human physiology. | 2016 | 27670826 |
| microbial biofilm community variation in flowing habitats: potential utility as bioindicators of postmortem submersion intervals. | biofilms are a ubiquitous formation of microbial communities found on surfaces in aqueous environments. these structures have been investigated as biomonitoring indicators for stream heath, and here were used for the potential use in forensic sciences. biofilm successional development has been proposed as a method to determine the postmortem submersion interval (pmsi) of remains because there are no standard methods for estimating the pmsi and biofilms are ubiquitous in aquatic habitats. we soug ... | 2016 | 27681897 |
| feasibility of electromagnetically guided transjugular intrahepatic portosystemic shunt procedure. | to develop an electromagnetic navigation technology for transjugular intrahepatic portosystemic shunt (tips) creation and translate it from phantom to an in-vivo large animal setting. | 2017 | 27686414 |
| flexible growing rods: a biomechanical pilot study of polymer rod constructs in the stability of skeletally immature spines. | surgical treatments for early onset scoliosis (eos) correct curvatures and improve respiratory function but involve many complications. a distractible, or 'growing rod,' implant construct that is more flexible than current metal rod systems may sufficiently correct curves in small children and reduce complications due to biomechanical factors. the purpose of this pilot study was to determine ranges of motion (rom) after implantation of simulated growing rod constructs with a range of clinically ... | 2016 | 27689140 |
| minimally invasive beat-by-beat monitoring of cardiac power in normal hearts and during acute ventricular dysfunction. | cardiac power, the product of aortic flow and blood pressure, appears to be a fundamental cardiovascular parameter. the simplified version named cardiac power output (cpo), calculated as the product of cardiac output (co) in l/min and mean arterial pressure (map) in mmhg divided by 451, has shown great ability to predict outcome in a broad spectrum of cardiac disease. beat-by-beat evaluation of cardiac power (pwr) therefore appears to be a possibly valuable addition when monitoring circulatory u ... | 2016 | 27702881 |
| frequency of vascular inflammation and impact on neointimal proliferation of drug eluting stents in porcine coronary arteries. | we aimed to describe the frequency of vascular inflammatory reactions with second generation drug eluting stents (des) compared to first generation des, and analyze the impact on inflammation and neointimal proliferation in a porcine coronary model. | 2016 | 27713606 |
| expressed alleles of imprinted igf2, dlk1 and meg3 colocalize in 3d-preserved nuclei of porcine fetal cells. | to explore the relationship between spatial genome organization and gene expression in the interphase nucleus, we used a genomic imprinting model, which offers parental-specific gene expression. using 3d fish in porcine fetal liver cells, we compared the nuclear organization of the two parental alleles (expressed or not) of insulin-like growth factor 2 (igf2), a paternally imprinted gene located on chromosome 2. we investigated whether its nuclear positioning favors specific locus associations. ... | 2016 | 27716032 |
| osteoblast-like cells with different embryologic origins behave differently in increasing zoledronic acid concentrations: a pilot study in pigs. | we compared the intraindividual effects of increasing zoledronic acid (za) concentrations on osteoblast-like cells with different embryologic origins. | 2017 | 27720655 |
| the habitat of coccidioides spp. and the role of animals as reservoirs and disseminators in nature. | coccidioidomycosis, a potentially fatal fungal infection, is considered an emergent mycotic disease because of the increased incidence of fungal infections registered over recent years. infection occurs through the inhalation of arthroconidia from two main species of coccidioides: coccidioides immitis and c. posadasii, which are both endemic to arid and semi-arid regions of north america. coccidioides species not only infect humans but can also infect other mammals (land, aquatic, wild or domest ... | 2016 | 27724885 |
| an in vivo multi-modal structural template for neonatal piglets using high angular resolution and population-based whole-brain tractography. | an increasing number of applications use the postnatal piglet model in neuroimaging studies, however, these are based primarily on t1 weighted image templates. there is a growing need for a multimodal structural brain template for a comprehensive depiction of the piglet brain, particularly given the growing applications of diffusion weighted imaging for characterizing tissue microstructures and white matter organization. in this study, we present the first multimodal piglet structural brain temp ... | 2016 | 27729850 |
| preclinical study of single-dose moxidectin, a new oral treatment for scabies: efficacy, safety, and pharmacokinetics compared to two-dose ivermectin in a porcine model. | scabies is one of the commonest dermatological conditions globally; however it is a largely underexplored and truly neglected infectious disease. foremost, improvement in the management of this public health burden is imperative. current treatments with topical agents and/or oral ivermectin (ivm) are insufficient and drug resistance is emerging. moxidectin (mox), with more advantageous pharmacological profiles may be a promising alternative. | 2016 | 27732588 |
| new experiments and a model-driven approach for interpreting middle stone age lithic point function using the edge damage distribution method. | the middle stone age (msa) is associated with early evidence for symbolic material culture and complex technological innovations. however, one of the most visible aspects of msa technologies are unretouched triangular stone points that appear in the archaeological record as early as 500,000 years ago in africa and persist throughout the msa. how these tools were being used and discarded across a changing pleistocene landscape can provide insight into how msa populations prioritized technological ... | 2016 | 27736886 |
| evaluation of the human adaptation of influenza a/h7n9 virus in pb2 protein using human and swine respiratory tract explant cultures. | novel avian h7n9 virus emerged in china in 2013 resulting in a case fatality rate of around 39% and continues to pose zoonotic and pandemic risk. amino acid substitutions in pb2 protein were shown to influence the pathogenicity and transmissibility of h7n9 following experimental infection of ferrets and mice. in this study, we evaluated the role of amino acid substitution pb2-627k or compensatory changes at pb2-591k and pb2-701n, on the tropism and replication competence of h7n9 viruses for huma ... | 2016 | 27739468 |
| between the vinča and linearbandkeramik worlds: the diversity of practices and identities in the 54th-53rd centuries cal bc in southwest hungary and beyond. | perhaps nowhere in european prehistory does the idea of clearly-defined cultural boundaries remain more current than in the initial neolithic, where the southeast-northwest trend of the spread of farming crosses what is perceived as a sharp divide between the balkans and central europe. this corresponds to a distinction between the vinča culture package, named for a classic site in serbia, with its characteristic pottery assemblage and absence of longhouses, and the linearbandkeramik (lbk), with ... | 2016 | 27746586 |
| biological effect on restenosis and vascular healing of encapsulated paclitaxel nanocrystals delivered via coated balloon technology in the familial hypercholesterolaemic swine model of in-stent restenosis. | the aim of this study was to evaluate the biological efficacy of a novel lower-dose (2.5 µg/mm2) encapsulated paclitaxel nanocrystal-coated balloon (nano-pcb) in the familial hypercholesterolaemic swine (fhs) model of iliofemoral in-stent restenosis. | 2016 | 27753601 |
| evidence for karyotype polymorphism in the free-living flatworm, macrostomum lignano, a model organism for evolutionary and developmental biology. | over the past decade, the free-living flatworm macrostomum lignano has been successfully used in many areas of biology, including embryology, stem cells, sexual selection, bioadhesion and aging. the increased use of this powerful laboratory model, including the establishment of genomic resources and tools, makes it essential to have a detailed description of the chromosome organization of this species, previously suggested to have a karyotype with 2n = 8 and one pair of large and three pairs of ... | 2016 | 27755577 |
| challenges for african swine fever vaccine development-"… perhaps the end of the beginning." | african swine fever (asf), an acute, viral hemorrhagic disease in domestic swine with mortality rates approaching 100%, is arguably the most significant emerging disease threat for the swine industry worldwide. devastating asf outbreaks and continuing epidemic in the caucasus region and russia (2007-to date) highlight significance of this disease threat. there is no vaccine for asf, thus leaving animal slaughter the only effective disease control option. it is clear, however, that vaccination is ... | 2016 | 27756505 |
| a usability comparison of laser suction handpieces for percutaneous nephrolithotomy. | the holmium laser has revolutionized the practice of minimally invasive endoscopy for kidney stones. recently, a novel, rigid handpiece for use in percutaneous nephrolithotomy (pcnl) that couples the holmium laser with suction has been developed. to date, limited data exist regarding the usability and ergonomics of such treatment systems. we thus sought to compare surgeon-rated usability with three different suction laser handpieces in a porcine model. | 2016 | 27758119 |
| leading edge analysis of transcriptomic changes during pseudorabies virus infection. | eight rna samples taken from the tracheobronchial lymph nodes (tbln) of pigs that were either infected or non-infected with a feral isolate of porcine pseudorabies virus (prv) were used to investigate changes in gene expression related to the pathogen. the rna was processed into fastq files for each library prior to being analyzed using illumina digital gene expression tag profiling sequences (dgetp) which were used as the downstream measure of differential expression. analyzed tags consisted of ... | 2016 | 27766207 |