Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| new insights into the role of ticks in african swine fever epidemiology. | african swine fever (asf), one of the most important diseases of swine, is present in many african countries, as well as in eastern europe, russia and sardinia. it is caused by a complex virus, asf virus (asfv), for which neither vaccine nor treatment is available. asfv affects swine of all breeds and ages, and also replicates in soft ticks of the genus ornithodoros, facilitating asfv persistence and reocurrence of disease. depending on the involvement of these ticks, and the presence or not of ... | 2015 | 26601452 |
| african swine fever virus georgia 2007 with a deletion of virulence-associated gene 9gl (b119l), when administered at low doses, leads to virus attenuation in swine and induces an effective protection against homologous challenge. | african swine fever virus (asfv) is the etiological agent of an often lethal disease of domestic pigs. disease control strategies have been hampered by the unavailability of vaccines against asfv. since its introduction in the republic of georgia, a highly virulent virus, asfv georgia 2007 (asfv-g), has caused an epizootic that spread rapidly into eastern european countries. currently no vaccines are available or under development to control asfv-g. in the past, genetically modified asfvs harbor ... | 2015 | 26063424 |
| [on the situation of african swine fever and the biological characterization of recent virus isolates]. | african swine fever (asf), a disease notifiable to the world organization of animal health (oie), is characterized by severe, unspecific clinical signs and high mortality rates. hosts for asf virus (asfv) are only members of the family suidae and soft ticks of the genus ornithodoros. currently, no vaccine is available and therefore, the control is primarily based on strict sanitary measures. the most important part is the early detection of the disease within affected animal holdings and the fas ... | 2015 | 26054220 |
| a longitudinal survey of african swine fever in uganda reveals high apparent disease incidence rates in domestic pigs, but absence of detectable persistent virus infections in blood and serum. | african swine fever (asf) is a fatal, haemorrhagic disease of domestic pigs, that poses a serious threat to pig farmers and is currently endemic in domestic pigs in most of sub-saharan africa. to obtain insight into the factors related to asf outbreaks at the farm-level, a longitudinal study was performed in one of the major pig producing areas in central uganda. potential risk factors associated with outbreaks of asf were investigated including the possible presence of apparently healthy asf-vi ... | 2015 | 25967670 |
| course and transmission characteristics of oral low-dose infection of domestic pigs and european wild boar with a caucasian african swine fever virus isolate. | in 2007, african swine fever virus (asfv) was introduced into the transcaucasian countries and russia. since then, it has spread alarmingly and reached the european union. asfv strains are highly virulent and lead to almost 100% mortality under experimental conditions. however, the possibility of dose-dependent disease courses has been discussed. for this reason, a study was undertaken to assess the risk of chronic disease and the establishment of carriers upon low-dose oronasal infection of dom ... | 2015 | 25916610 |
| african swine fever virus georgia isolate harboring deletions of mgf360 and mgf505 genes is attenuated in swine and confers protection against challenge with virulent parental virus. | african swine fever virus (asfv) is the etiological agent of a contagious and often lethal disease of domestic pigs that has significant economic consequences for the swine industry. the control of african swine fever (asf) has been hampered by the unavailability of vaccines. experimental vaccines have been developed using genetically modified live attenuated asfvs where viral genes involved in virus virulence were removed from the genome. multigene family 360 (mgf360) and mgf505 represent a gro ... | 2015 | 25810553 |
| a study of lymphoid organs and serum proinflammatory cytokines in pigs infected with african swine fever virus genotype ii. | african swine fever virus (asfv), the causative agent of one of the most important viral diseases of domestic pigs for which no vaccine is available, causes immune system disorders in infected animals. in this study, the serum levels of proinflammatory cytokines, as well as the histological and cellular constitution of lymphoid organs of pigs infected with asfv genotype ii were investigated. the results showed a high degree of lymphocyte depletion in the lymphoid organs, particularly in the sple ... | 2015 | 25797197 |
| the progressive adaptation of a georgian isolate of african swine fever virus to vero cells leads to a gradual attenuation of virulence in swine corresponding to major modifications of the viral genome. | african swine fever virus (asfv) causes a contagious and often lethal disease of feral and domestic swine. experimental vaccines derived from naturally occurring, genetically modified, or cell culture-adapted asfv have been evaluated, but no commercial vaccine is available to control african swine fever (asf). we report here the genotypic and phenotypic analysis of viruses obtained at different passages during the process of adaptation of a virulent asfv field isolate from the republic of georgi ... | 2015 | 25505073 |
| alaria alata mesocercariae among feral cats and badgers, denmark. | 2015 | 26402302 | |
| chinese border disease virus strain jsls12-01 infects piglets and down-regulates the antibody responses of classical swine fever virus c strain vaccination. | during 2012 and 2013, several border disease virus (bdv) strains were identified from chinese goat and sheep herds. at the same time, pigs from the same areas were found to be seropositive to bdv by elisa, without showing clinical signs (unpublished data). to examine the susceptibility of pigs to the chinese bdv strains, bdv isolate jsls12-01, isolated from naturally infected sheep, was used to infect pigs. antibody responses, viremia, clinical signs and pathological changes of the infected anim ... | 2015 | 26117151 |
| a serosurvey for brucellosis in wild boar (sus scrofa) in sardinia, italy. | porcine brucellosis is a zoonotic disease caused by brucella suis and hosted by pigs (sus scrofa). both domestic pigs and wild boars are affected. we measured the prevalence of antibody to brucella spp. in wild boars in sardinia, italy. during 1 november 2009 to 31 january 2010, we collected 570 serum samples from legally hunted wild boars and tested them using a commercial competitive enzyme-linked immunosorbent assay. sex and age class of the sampled wild boars were also recorded. thirty-five ... | 2015 | 26267463 |
| brucella suis infection in domestic pigs in sardinia (italy). | during a 4-year (2007-2010) survey, the presence of brucella suis infection in domestic pigs in sardinia was investigated. serum samples were collected from breeding pigs located on 108 commercial farms with documented reproductive problems and analysed using the rose bengal (rbt) and complement fixation (cft) tests for screening and confirmation of brucella, respectively. of the 1251 serum samples analysed by rbt, 406 sera, originating from 36 farms, were positive for b. suis. cft was positive ... | 2015 | 25530227 |
| the porcine tspy gene is tricopy but not a copy number variant. | the testis-specific protein y-encoded (tspy) gene is situated on the mammalian y-chromosome and exhibits some remarkable biological characteristics. it has the highest known copy number (cn) of all protein coding genes in the human and bovine genomes (up to 74 and 200, respectively) and also shows high individual variability. although the biological function of tspy has not yet been elucidated, its specific expression in the testis and several identified binding domains within the protein sugges ... | 2015 | 26133983 |
| species differences in the geometry of the anterior segment differentially affect anterior chamber cell scoring systems in laboratory animals. | to determine the impact of anterior segment geometry on ocular scoring systems quantifying anterior chamber (ac) cells in humans and 7 common laboratory species. | 2015 | 26539819 |
| sexually antagonistic selection during parental care is not generated by a testosterone-related intralocus sexual conflict-insights from full-sib comparisons. | the evolution of shared male and female traits can be hampered if selection favours sex-specific optima. however, such genomic conflicts can be resolved when independent male and female mechanisms evolve. the existence, extent and consequences of conflict and/or conflict resolution are currently debated. endocrinological traits like plasma testosterone (t) are suitable test cases, given their important role in mediating correlated traits, plus their opposing sex-specific fitness effects. we comp ... | 2015 | 26625951 |
| a breath of fresh air: a quality-improvement study comparing an air-circulating technique versus conventional technique to prevent nasogastric tube dysfunction. | nasogastric tubes are an important component of care in patients with gastrointestinal obstructions. however, they are prone to malfunction despite conventional flushing techniques, with potentially severe consequences. there is no widely accepted, gold-standard way to ensure that a nasogastric tube succeeds in maintaining an empty stomach following flushing. | 2015 | 26617465 |
| a comparative study of the morphology of mammalian chordae tendineae of the mitral and tricuspid valves. | it is assumed that the human heart is almost identical to domestic mammalian species, but with limited literature to support this. one such area that has been underinvestigated is that of the subvalvular apparatus level. the authors set out to examine the morphology of the subvalvular apparatus of the mammalian atrioventricular valves through gross dissection and microscopic analysis in a small-scale pilot study. the authors examined the chordae tendineae of the mitral and tricuspid valves in sh ... | 2015 | 26644912 |
| selection and validation of reference genes for qrt-pcr expression analysis of candidate genes involved in olfactory communication in the butterfly bicyclus anynana. | real-time quantitative reverse transcription pcr (qrt-pcr) is a technique widely used to quantify the transcriptional expression level of candidate genes. qrt-pcr requires the selection of one or several suitable reference genes, whose expression profiles remain stable across conditions, to normalize the qrt-pcr expression profiles of candidate genes. although several butterfly species (lepidoptera) have become important models in molecular evolutionary ecology, so far no study aimed at identify ... | 2015 | 25793735 |
| identification of specialists and abundance-occupancy relationships among intestinal bacteria of aves, mammalia, and actinopterygii. | the coalescence of next-generation dna sequencing methods, ecological perspectives, and bioinformatics analysis tools is rapidly advancing our understanding of the evolution and function of vertebrate-associated bacterial communities. delineation of host-microbe associations has applied benefits ranging from clinical treatments to protecting our natural waters. microbial communities follow some broad-scale patterns observed for macroorganisms, but it remains unclear how the specialization of int ... | 2015 | 26712546 |
| mechanistic insights into glucan phosphatase activity against polyglucan substrates. | glucan phosphatases are central to the regulation of starch and glycogen metabolism. plants contain two known glucan phosphatases, starch excess4 (sex4) and like sex four2 (lsf2), which dephosphorylate starch. starch is water-insoluble and reversible phosphorylation solubilizes its outer surface allowing processive degradation. vertebrates contain a single known glucan phosphatase, laforin, that dephosphorylates glycogen. in the absence of laforin, water-soluble glycogen becomes insoluble, leadi ... | 2015 | 26231210 |
| the evolution of stomach acidity and its relevance to the human microbiome. | gastric acidity is likely a key factor shaping the diversity and composition of microbial communities found in the vertebrate gut. we conducted a systematic review to test the hypothesis that a key role of the vertebrate stomach is to maintain the gut microbial community by filtering out novel microbial taxa before they pass into the intestines. we propose that species feeding either on carrion or on organisms that are close phylogenetic relatives should require the most restrictive filter (meas ... | 2015 | 26222383 |
| hypoxanthine-guanine phosphoribosyltransferase and inosine 5'-monophosphate dehydrogenase activities in three mammalian species: aquatic (mirounga angustirostris), semi-aquatic (lontra longicaudis annectens) and terrestrial (sus scrofa). | aquatic and semiaquatic mammals have the capacity of breath hold (apnea) diving. northern elephant seals (mirounga angustirostris) have the ability to perform deep and long duration dives; during a routine dive, adults can hold their breath for 25 min. neotropical river otters (lontra longicaudis annectens) can hold their breath for about 30 s. such periods of apnea may result in reduced oxygen concentration (hypoxia) and reduced blood supply (ischemia) to tissues. production of adenosine 5'-tri ... | 2015 | 26283971 |
| haemodynamic differences between pancuronium and vecuronium in an experimental pig model. | to compare baseline cardiovascular function in anesthetised pigs using either pancuronium or vecuronium as a neuromuscular blocker. | 2015 | 24985148 |
| histopathological comparative analysis of peri-implant soft tissue response after dental implant placement with flap and flapless surgical technique. experimental study in pigs. | the aim of this study was comparing the effect of flapless vs. flap technique of implant placement on inflammation degree of peri-implant soft tissue, through histopathological analysis. | 2015 | 25039366 |
| genome-wide analysis reveals artificial selection on coat colour and reproductive traits in chinese domestic pigs. | pigs from asia and europe were independently domesticated from c. 9000 years ago. during this period, strong artificial selection has led to dramatic phenotypic changes in domestic pigs. however, the genetic basis underlying these morphological and behavioural adaptations is relatively unknown, particularly for indigenous chinese pigs. here, we performed a genome-wide analysis to screen 196 regions with selective sweep signals in tongcheng pigs, which are a typical indigenous chinese breed. gene ... | 2015 | 25132237 |
| active dynamic infrared thermal imaging in burn depth evaluation. | the aim of this study was to find the relationship between active dynamic thermography (adt) with cold excitation and burn depth. this new modality of evaluation of burns seems to be an attractive proposal for quantitative classification, allowing proper choice of burn wound treatment: conservative or surgical, especially compared with static thermography. the work was an in vivo experiment on domestic pigs, and a small number of patients were also diagnosed as part of the study. statistical ana ... | 2015 | 25144810 |
| mitochondrial dna sequence and phylogenetic evaluation of geographically disparate sus scrofa breeds. | next generation sequencing of mitochondrial dna (mtdna) facilitates studies into the metabolic characteristics of production animals and their relation to production traits. sequence analysis of mtdna from pure-bred swine with highly disparate production characteristics (mangalica blonde, mangalica swallow-bellied, meishan, turopolje, and yorkshire) was initiated to evaluate the influence of mtdna polymorphisms on mitochondrial function. herein, we report the complete mtdna sequences of five sus ... | 2015 | 25153451 |
| voluderm microneedle technology for skin treatments-in vivo histological evidence. | the growing demand for skin rejuvenation procedures with minimal down time and low risk has led to the development of fractional radiofrequency (rf) systems. the new voluderm™ rf microneedle technology creates minute columns of tissue thermal microablation. treatment triggers natural fractional healing, resulting in dermal volumizing and skin renewal. this preclinical research assessed the safety and efficacy of the voluderm through histological evaluation of morphological changes in the target ... | 2015 | 25151912 |
| efficacy and safety of novel epicardial circumferential left atrial ablation with pulmonary vein isolation in sustained atrial fibrillation. | the aim of this study was to examine the efficacy and safety of this novel epicardial circumferential left atrial ablation (claa) with pulmonary vein isolation (pvi) in sustained atrial fibrillation (af). thirty domestic pigs were divided equally into 3 groups: af without ablation (af group), af with pvi (pvi group), and af with claa and pvi (claa + pvi group). af was induced by rapid atrial pacing. after af was induced, claa and pvi were performed for pigs in claa + pvi group, and pvi was perfo ... | 2015 | 25323730 |
| immunohistochemical evidence of leptin and its receptor in the carpal glands of domestic pigs and wild boar. | in recent years, the protein hormone leptin has been the subject of numerous studies designed to clarify and interpret its functional significance; it has been speculated that this goes well beyond the control of appetite and energy metabolism. in particular, the presence of leptin and its receptor has been observed in various glands anatomically and functionally related to the reproductive apparatus. this has led to the hypothesis that leptin may act directly in the functional control of these ... | 2015 | 25292333 |
| generation of crispr/cas9-mediated gene-targeted pigs via somatic cell nuclear transfer. | the domestic pig has been widely used as an important large animal model. precise and efficient genetic modification in pig provides a great promise in biomedical research. recently, clustered regularly interspaced short palindromic repeat (crispr)/crispr-associated (cas) system has been successfully used to produce many gene-targeted animals. however, these animals have been generated by co-injection of cas9 mrna and single-guide rna (sgrna) into one-cell stage embryos, which mostly resulted in ... | 2015 | 25274063 |
| safety and biocompatibility of a novel self-expanding nitinol carotid stent with hybrid cell design in a porcine model of neointimal hyperplasia. | stent design may influence the outcomes, suggesting that adverse event rates vary according to free cell area and cell design. open cell design technology of self-expandable stents, dedicated for carotid revascularisation has better deliverability, although closed cell technology is expected to cause fewer thromboembolic events. | 2015 | 25371312 |
| advances in research in animal models of burn-related hypertrophic scarring. | skin burn injuries affect approximately 500,000 people per year in france. after deep burns, functional sequelae associated with hypertrophic and retractile scars are an important public health problem. to understand the pathophysiology of sequelae and evaluate new therapeutic approaches, the use of animal models that should be standard tools is necessary. some pre-clinical models of hypertrophic scars after burns have been described, but the choice of the appropriate and relevant experimental m ... | 2015 | 25356852 |
| a tourniquet assisted cardiopulmonary resuscitation augments myocardial perfusion in a porcine model of cardiac arrest. | during cardiopulmonary resuscitation (cpr), myocardial blood flow generated by chest compression rarely exceeds 35% of its normal level. cardiac output generated by chest compression decreases gradually with the prolongation of cardiac arrest and resuscitation. early studies have demonstrated that myocardial blood flow during cpr is largely dependent on peripheral vascular resistance. in this study, we investigated the effects of chest compression in combination with physical control of peripher ... | 2015 | 25447436 |
| evaluation of a commercial elisa for the detection of antibodies to sarcoptes scabiei in wild boar (sus scrofa). | sarcoptic mange occurs in free-ranging wild boar (sus scrofa) but has been poorly described in this species. we evaluated the performance of a commercial indirect enzyme-linked immunosorbent assay (elisa) for serodiagnosis of sarcoptic mange in domestic swine when applied to wild boar sera. we tested 96 sera from wild boar in populations without mange history ("truly noninfected") collected in switzerland between december 2012 and february 2014, and 141 sera from free-ranging wild boar presentin ... | 2015 | 26161723 |
| preliminary observations of arthropods associated with buried carrion on oahu. | several studies in hawaii have focused on arthropod succession and decomposition patterns of surface remains, but the current research presents the first study to focus on shallow burials in this context. three domestic pig carcasses (sus scrofa l.) were buried at the depths of 20-40 cm in silty clay loam soil on an exposed ridge on the leeward side of the volcanically formed koolau mountain range. one carcass was exhumed after 3 weeks, another after 6 weeks, and the last carcass was exhumed aft ... | 2015 | 25413711 |
| evaluation of the distribution of paclitaxel by immunohistochemistry and nuclear magnetic resonance spectroscopy after the application of a drug-eluting balloon in the porcine ureter. | the urothelium represents "the tightest and most impermeable barrier in the body." we investigated the distribution of paclitaxel (ptx) in the ureteral wall after the inflation of a paclitaxel-eluting balloon (peb) in an attempt to elucidate the possibility of clinical application of pebs in the ureter. | 2015 | 25441059 |
| submucosal endoscopy as an aid to full-thickness resection: pilot study in the porcine stomach. | endoscopic full-thickness resection (eftr) is commonly performed with laparoscopic assistance. submucosal endoscopy with full-thickness resection (seftr) is a new technique that combines submucosal endoscopy with the mucosal safety valve flap method to enable eftr. | 2015 | 25440673 |
| two-in-one aortic valve sizing and valvuloplasty conductance balloon catheter. | inaccurate aortic valve sizing and selection is linked to paravalvular leakage in transcatheter aortic valve replacement (tavr). here, a novel sizing valvuloplasty conductance balloon (svcb) catheter is shown to be accurate, reproducible, unbiased, and provides real-time tool for aortic valve sizing that fits within the standard valvuloplasty procedure. | 2015 | 25510238 |
| comparison of osteoconductive properties of three different β-tricalcium phosphate graft materials: a pilot histomorphometric study in a pig model. | the aim of this study was to compare the de novo bone formation ability and osteoconductive effects of three different β-tricalcium phosphate (β-tcp) graft materials. the micro-architectural parameters of the newly formed bone tissues were also compared among the different graft materials. | 2015 | 25491275 |
| sodium nitroprusside-enhanced cardiopulmonary resuscitation facilitates intra-arrest therapeutic hypothermia in a porcine model of prolonged ventricular fibrillation. | the aim of this study was to assess the effect of sodium nitroprusside-enhanced cardiopulmonary resuscitation on heat exchange during surface cooling. we hypothesized that sodium nitroprusside-enhanced cardiopulmonary resuscitation would decrease the time required to reach brain temperature less than 35°c compared to active compression-decompression plus impedance threshold device cardiopulmonary resuscitation alone, in the setting of intra-cardiopulmonary resuscitation cooling. we further hypot ... | 2015 | 25525755 |
| ccaat-enhancer binding protein (c/ebp) β regulates insulin-like growth factor (igf) 1 expression in porcine liver during prenatal and postnatal development. | igf1 expression regulation attracts numerous interests because of its important role during mammalian growth and development. domestic pig can be used as a valuable animal model to investigate human development since they share the high similarity in general physiology and metabolism. in this study, we examined the expression pattern of igf1 and found it associated with liver c/ebp β expression pattern in porcine liver during embryonic and postnatal development. both igf1 and c/ebp β expression ... | 2015 | 25543525 |
| pathological and molecular diagnosis of the 2013 african swine fever outbreak in lusaka, zambia. | african swine fever (asf) is a highly contagious and fatal hemorrhagic viral disease of domestic pigs. the disease is widespread in sub-saharan africa and has repeatedly been introduced into other continents. the current study describes the diagnostic investigations of a hemorrhagic disease that was reported in pigs in lusaka (october 2013), zambia. necropsy, histopathology, and molecular diagnosis using polymerase chain reaction and sequence analysis confirmed the disease to be asf. the sequenc ... | 2015 | 25535151 |
| adipose tissue remodeling in a novel domestic porcine model of diet-induced obesity. | to establish and characterize a novel domestic porcine model of obesity. | 2015 | 25627626 |
| quantitative characterization of endothelial cell morphologies depending on shear stress in different blood vessels of domestic pigs using a focused ion beam and high resolution scanning electron microscopy (fib-sem). | microstructured surfaces mimicking the endothelial cell (ec) morphology is a new approach to improve the blood compatibility of synthetic vascular grafts. the ecs are capable of changing their shapes depending on different shear conditions. however, the quantitative correlation between ec morphology and shear stress has not yet been investigated statistically. the aim of this study was to quantitatively investigate the morphology of ecs in dependence on the shear stress. blood flow rates in diff ... | 2015 | 25622890 |
| adaptation and possible ancient interspecies introgression in pigs identified by whole-genome sequencing. | domestic pigs have evolved genetic adaptations to their local environmental conditions, such as cold and hot climates. we sequenced the genomes of 69 pigs from 15 geographically divergent locations in china and detected 41 million variants, of which 21 million were absent from the dbsnp database. in a genome-wide scan, we identified a set of loci that likely have a role in regional adaptations to high- and low-latitude environments within china. intriguingly, we found an exceptionally large (14- ... | 2015 | 25621459 |
| liposarcoma in animals: literature review and case report in a domestic pig (sus scrofa). | liposarcomas are malignant tumors of adipocytes. the current report describes a liposarcoma in a 2.5-year-old, mixed-breed commercial sow that was detected during meat inspection. on gross examination, a firm, whitish, multinodular, 20 cm ×10 cm mass was observed in the perirenal area along with smaller nodules multifocally scattered within the renal parenchyma. histological examination revealed an anaplastic sarcoma with clear intracytoplasmic lipidic vacuoles that were positive for sudan black ... | 2015 | 25613042 |
| light microscopy and scanning electron microscopy study on microstructure of gallbladder mucosa in pig. | the present light microscopy (lm) and scanning electron microscopy (sem) studies on porcine gallbladder mucosa provide a description of the microstructures of great functional importance such as mucosal folds, the epithelium, glands, and lymphatic nodules. the results showed the regional structural differences of the porcine gallbladder wall. depending on the part of the gallbladder, three types of mucosal structures were described: simple and branched folds and mucosal crypts. an important stru ... | 2015 | 25604381 |
| profiles of reproductive hormone in the microminipig during the normal estrous cycle. | we investigated changes in the peripheral plasma concentrations of ovarian steroids and gonadotropins throughout the estrous cycle of the microminipig (mmpig), a novel experimental animal model. the mean (± standard error of the mean (sem)) duration of estrous and the estrous cycle in seven animals was 66.0 ± 5.2 h and 20.6 ± 0.3 days, respectively. a luteinizing hormone (lh) surge was observed 16.5 ± 6.5 h after the onset of estrous in six out of the seven animals. the lh peak and the duration ... | 2015 | 25600524 |
| qtl for seed protein and amino acids in the benning × danbaekkong soybean population. | we identified qtl associated with protein and amino acids in a soybean mapping population that was grown in five environments. these qtl could be used in mas to improve these traits. soybean, rather than nitrogen-containing forages, is the primary source of quality protein in feed formulations for domestic swine, poultry, and dairy industries. as a sole dietary source of protein, soybean is deficient in the amino acids lysine (lys), threonine (thr), methionine (met), and cysteine (cys). increasi ... | 2015 | 25673144 |
| genetic variation of the east balkan swine (sus scrofa) in bulgaria, revealed by mitochondrial dna and y chromosomal dna. | east balkan swine (ebs) sus scrofa is the only aboriginal domesticated pig breed in bulgaria and is distributed on the western coast of the black sea in bulgaria. to reveal the breed's genetic characteristics, we analysed mitochondrial dna (mtdna) and y chromosomal dna sequences of ebs in bulgaria. nucleotide diversity (πn ) of the mtdna control region, including two newly found haplotypes, in 54 ebs was higher (0.014 ± 0.007) compared with that of european (0.005 ± 0.003) and asian (0.006 ± 0.0 ... | 2015 | 25662601 |
| the effects of hemorrhagic shock secondary to hepatectomy in a swine model. | ischemia-reperfusion injury caused by severe hemorrhagic shock and subsequent resuscitation leads to deterioration of hepatic homeostasis and possibly to liver failure. the present study focuses on determining whether there is a different biological response to hemorrhagic shock by different sources of hemorrhage, hepatic hemorrhage (hh) versus peripheral hemorrhage. | 2015 | 25659614 |
| mechanism by which shock wave lithotripsy can promote formation of human calcium phosphate stones. | human stone calcium phosphate (cap) content correlates with higher urine cap supersaturation (ss) and urine ph as well as with the number of shock wave lithotripsy (swl) treatments. swl does damage medullary collecting ducts and vasa recta, sites for urine ph regulation. we tested the hypothesis that swl raises urine ph and therefore cap ss, resulting in cap nucleation and tubular plugging. the left kidney (t) of nine farm pigs was treated with swl, and metabolic studies were performed using bil ... | 2015 | 25656372 |
| domestic pigs' (sus scrofa domestica) use of direct and indirect visual and auditory cues in an object choice task. | recently, foraging strategies have been linked to the ability to use indirect visual information. more selective feeders should express a higher aversion against losses compared to non-selective feeders and should therefore be more prone to avoid empty food locations. to extend these findings, in this study, we present a series of studies investigating the use of direct and indirect visual and auditory information by an omnivorous but selective feeder-the domestic pig. subjects had to choose bet ... | 2015 | 25650328 |
| inhibition of nitric oxide synthases, but not inducible nitric oxide synthase, selectively worsens left ventricular function after successful resuscitation from cardiac arrest in swine. | nitric oxide (no) is a critical regulator of vascular tone and signal transduction in the cardiovascular system. no is synthesized by three unique enzymes (nitric oxide synthases [nos]): endothelial and neuronal nos, both constitutively expressed, and inducible nos (inos), which is induced by proinflammatory stimuli and subsequently produces a burst of no. no has been implicated as both an injurious and a beneficial mediator after cardiac arrest and resuscitation. a previous study in swine found ... | 2015 | 25639298 |
| tissue concentrations of sulfamethazine and tetracycline hydrochloride of swine (sus scrofa domestica) as it relates to withdrawal methods for international export. | the use of water medications is a common practice in the us swine industry to treat and prevent infections in swine herds with minimal labor and without risk of needle breakage. there are concerns that fda-approved withdrawal times (wdt) may be inadequate for several water medications when exporting pork products to countries where mrls (maximum residue limits) are lower than us tolerance levels. in this study, withdrawal intervals (wdi) were estimated for pigs when dosed with tetracycline and s ... | 2015 | 25707857 |
| evaluation of the plasmatic and parenchymal elution kinetics of two different irinotecan-loaded drug-eluting embolics in a pig model. | to evaluate and compare irinotecan elution kinetics of two drug-eluting embolic agents in a porcine model. | 2015 | 25704223 |
| changes in lens stiffness due to capsular opacification in accommodative lens refilling. | accommodation may be restored to presbyopic lenses by refilling the lens capsular bag with a soft polymer. after this accommodative lens refilling prevention of capsular opacification is a requirement, since capsular opacification leads to a decreased clarity of the refilled lens. it has been hypothesized that capsular fibrosis causing the capsular opacification results in increased stiffness of the lens capsular bag, therewith contributing to a decrease in accommodative amplitude of the lens. h ... | 2015 | 25704214 |
| bipolar multi-electrode balloon catheter radiofrequency renal denervation with the vessix system: preclinical safety evaluation. | a bipolar multi-electrode 7 fr-compatible balloon-catheter radiofrequency (rf) renal denervation system (vessix™ renal denervation system; boston scientific, marlborough, ma, usa) was evaluated for safety in domestic swine. | 2015 | 25701511 |
| cardiac remodeling in a new pig model of chronic heart failure: assessment of left ventricular functional, metabolic, and structural changes using pet, ct, and echocardiography. | large animal models are needed to study disease mechanisms in heart failure (hf). in the present study we characterized the functional, metabolic, and structural changes of myocardium in a novel pig model of chronic myocardial infarction (mi) by using multimodality imaging and histology. | 2015 | 25698475 |
| randomized trial on the physiologic impact of notes. | our previous work revealed significantly less acidosis in swine undergoing natural orifice translumenal endoscopic surgery (notes) using endoscopic air insufflation than swine undergoing standard laparoscopy. we wanted to evaluate the differential effects of co2 versus intra-abdominal pressure as source for this finding. in addition, we investigated the endocrine stress response between swine undergoing notes peritoneoscopy with co2 insufflation and animals undergoing standard diagnostic laparos ... | 2015 | 25698397 |
| a taphonomic study exploring the differences in decomposition rate and manner between frozen and never frozen domestic pigs (sus scrofa). | this research examined differences in decomposition rate and manner of domestic pig subjects (sus scrofa) in never frozen (control) and previously frozen (experimental) research conditions. eight control and experimental subjects were placed in an identical outdoor research environment. daily quantitative and qualitative measurements were collected: abdominal circumference, total body score (tbs), temperature, photographs, descriptive decomposition stages, and visual observations. field necropsi ... | 2015 | 25693630 |
| modeling cardiac arrest and resuscitation in the domestic pig. | cardiac arrest remains a leading cause of death and permanent disability worldwide. although many victims are initially resuscitated, they often succumb to the extensive ischemia-reperfusion injury inflicted on the internal organs, especially the brain. cardiac arrest initiates a complex cellular injury cascade encompassing reactive oxygen and nitrogen species, ca(2+) overload, atp depletion, pro- and anti-apoptotic proteins, mitochondrial dysfunction, and neuronal glutamate excitotoxity, which ... | 2015 | 25685718 |
| transgastric endoscopic gastrojejunostomy using holing followed by interrupted suture technique in a porcine model. | to demonstrate the feasibility and reproducibility of a pure natural orifice transluminal endoscopic surgery (notes) gastrojejunostomy using holing followed by interrupted suture technique using a single endoloop matched with a pair of clips in a non-survival porcine model. | 2015 | 26504508 |
| lungs exposed to 1 hour warm ischemia without heparin before harvesting might be suitable candidates for transplantation. | the limiting factor for lung transplantation is the lack of donor organs. the usage of lungs from donation after cardiac death (dcd) would dramatically increase donor availability. in the present paper we wanted to investigate lungs exposed to 1 h of warm ischemia without heparin followed by flush-perfusion and cold storage compared to lungs harvested from heart beating donors (hbd) using standard harvesting technique. | 2015 | 26500001 |
| intraindividual comparison of image quality using retrospective and prospective respiratory gating for the acquisition of thin sliced four dimensional multidetector ct of the thorax in a porcine model. | to intraindividually compare image quality and anatomical depiction of the lung and mediastinum using retrospective and prospective respiratory gating techniques for the acquisition of 4d-multidetector computed tomography (mdct) of the chest in a porcine model. | 2015 | 26495957 |
| effects of hydrogen sulfide (h2s) on mesenteric perfusion in experimental induced intestinal ischemia in a porcine model. | insufficient mesenteric perfusion is a dramatic complication in critically ill patients. hydrogen sulfide, a newly recognized endogenous gaseous mediator, acts as an intestinal vasoactive agent and seems to protect against mesenteric ischemic damage. we investigated whether sodium hydrogen sulfide, a hydrogen sulfide donor, can improve mesenteric perfusion in an experimental model of pigs, both in physiological and ischemic conditions. | 2015 | 26495269 |
| pulse oximetry: a non-invasive, novel marker for the quality of chest compressions in porcine models of cardiac arrest. | pulse oximetry, which noninvasively detects the blood flow of peripheral tissue, has achieved widespread clinical use. we have noticed that the better the quality of cardiopulmonary resuscitation (cpr), the better the appearance of pulse oximetry plethysmographic waveform (pop). we investigated whether the area under the curve (auc) and/or the amplitude (amp) of pop could be used to monitor the quality of cpr. | 2015 | 26485651 |
| morphological aspects and physical properties of enamel and dentine of sus domesticus: a tooth model in laboratory research. | this study aims to describe and analyze morphological and physical properties of deciduous teeth of sus domesticus. ultrastructural analysis, mineral composition and microhardness of enamel and dentine tissues were performed on 10 skulls of s. domesticus. external anatomic characteristics and the internal anatomy of the teeth were also described. data regarding microhardness and ultrastructural analysis were subjected to statistical tests. for ultrastructural analysis, we used the analysis of va ... | 2015 | 26434756 |
| experimental transmission of african swine fever (asf) low virulent isolate nh/p68 by surviving pigs. | african swine fever (asf) has persisted in eastern europe since 2007, and two endemic zones have been identified in the central and southern parts of the russian federation. moderate- to low-virulent asf virus isolates are known to circulate in endemic asf-affected regions. to improve our knowledge of virus transmission in animals recovered from asf virus infection, an experimental in vivo study was carried out. four domestic pigs were inoculated with the nh/p68 asf virus, previously characteriz ... | 2015 | 26432068 |
| evolution of coronary flow in an experimental slow flow model in swines: angiographic and pathological insights. | pathomechanism of coronary slow flow phenomenon remains largely unclear now. present study observed the pathological and angiographic evolution in a pig model of coronary slow flow. | 2015 | 26539516 |
| developing a kinematic understanding of chest compressions: the impact of depth and release time on blood flow during cardiopulmonary resuscitation. | effective cardiopulmonary resuscitation is a critical component of the pre-hospital treatment of cardiac arrest victims. mechanical chest compression (mcc) devices enable the delivery of mcc waveforms that could not be delivered effectively by hand. while chest compression generated blood flow has been studied for more than 50 years, the relation between sternum kinematics (depth over time) and the resulting blood flow have not been well described. using a five parameter mcc model, we studied th ... | 2015 | 26537881 |
| experimental study of kinematic activity of left ventricular myocardium. | a method of invasive and non-invasive examination of epicardial motion was tested on domestic pigs (n=15). the invasive study employed a contact kinematic sensor of the epicardial wall motion, which determined the amplitude, velocity, and angular parameters of examined epicardial region. a non-invasive assessment of the epicardial kinematics based on echocardiographic 2d velocity vector imaging and ecg recoding used to determine the heart electrical axis. the 4-chamber views were uses to analyze ... | 2015 | 26531214 |
| [uterine neoplasia in two geriatric miniature pet pigs - diagnostics and therapy. two case reports]. | miniature pet pigs are becoming ever more common among the patients of small animal practices. due to an advanced age attained in these animals in comparison with conventional pigs in pork production, miniature pet pigs develop age-related diseases that are infrequently observed in farm pigs, including neoplastic alterations of the uterus. we describe two cases of a uterine adenocarcinoma and a leiomyoma, respectively, in two miniature pet pig sows. options and limits of diagnostic measures (uns ... | 2015 | 26530159 |
| changes in saliva biomarkers of stress and immunity in domestic pigs exposed to a psychosocial stressor. | a combination of salivary biomarkers measured at the same time, reflecting the different systems that are involved in the stress mechanism, could be the best tool for its evaluation. in this study, changes in a panel of salivary biomarkers of stress and immunity including chromogranin a (cga), iga, cortisol, testosterone, haptoglobin (hp) and c-reactive protein (crp), were evaluated. a total of 14 (7 control and 7 test) crossbred duroc × (landrace × large white) males of 190 days of age were use ... | 2015 | 26412517 |
| human recombinant activated protein c-coated stent for the prevention of restenosis in porcine coronary arteries. | activated protein c (apc), an endogenous protein, inhibits inflammation and thrombosis and interrupts the coagulation cascade. here, we investigated the effect of human recombinant apc on the development of neointimal hyperplasia in porcine coronary arteries. yukon choice bare metal stents were coated with 2.6 µg apc/mm(2). under general anesthesia, apc-coated and bare stents were implanted in the left anterior descending and circumflex coronary arteries of 10 domestic pigs. during the 4-week fo ... | 2015 | 26411437 |
| blood collection from the orbital sinus of swine. | swine (sus scrofa domesticus) are increasingly used as animal models in biomedical research. often blood samples from these animals are needed for research protocols, but phlebotomy can be difficult, especially with young swine. this column describes a relatively simple technique for blood collection that can be used specifically with young swine. | 2015 | 26398611 |
| longitudinal study of the early-life fecal and nasal microbiotas of the domestic pig. | the mammalian microbiota plays a key role in host health and disease susceptibility. however, knowledge of the early-age microbiota of pigs is lacking. the purpose of this study was to use high-throughput next-generation sequencing to characterize the fecal and nasal microbiotas of pigs during early life. | 2015 | 26391877 |
| coronary injury score correlates with proliferating cells and alpha-smooth muscle actin expression in stented porcine coronary arteries. | neointimal formation and cell proliferation resulting into in-stent restenosis is a major pathophysiological event following the deployment of stents in the coronary arteries. in this study, we assessed the degree of injury, based on damage to internal elastic lamina, media, external elastic lamina, and adventitia following the intravascular stenting, and its relationship with the degree of smooth muscle cell proliferation. we examined the smooth muscle cell proliferation and their phenotype at ... | 2015 | 26382957 |
| soil fungal community shift evaluation as a potential cadaver decomposition indicator. | fungi metabolise organic matter in situ and so alter both the bio-/physico-chemical properties and microbial community structure of the ecosystem. in particular, they are responsible reportedly for specific stages of decomposition. therefore, this study aimed to extend previous bacteria-based forensic ecogenomics research by investigating soil fungal community and cadaver decomposition interactions in microcosms with garden soil (20 kg, fresh weight) and domestic pig (sus scrofa domesticus) carc ... | 2015 | 26322496 |
| characterization of a porcine model for associating liver partition and portal vein ligation for a staged hepatectomy. | publications using the alpps (associating liver partition and portal vein ligation for a staged hepatectomy) procedure have demonstrated a future liver remnant growth of 40-160% in only 6-9 days. the present study aimed to develop and describe the first large animal model of alpps that can be used for future studies. | 2015 | 26234167 |
| segmental and age differences in the elastin network, collagen, and smooth muscle phenotype in the tunica media of the porcine aorta. | the porcine aorta is often used in studies on morphology, pathology, transplantation surgery, vascular and endovascular surgery, and biomechanics of the large arteries. using quantitative histology and stereology, we estimated the area fraction of elastin, collagen, alpha-smooth muscle actin, vimentin, and desmin within the tunica media in 123 tissue samples collected from five segments (thoracic ascending aorta; aortic arch; thoracic descending aorta; suprarenal abdominal aorta; and infrarenal ... | 2015 | 26232584 |
| influence of internal fixation systems on radiation therapy for spinal tumor. | in this study, the influence of internal fixation systems on radiation therapy for spinal tumor was investigated in order to derive a theoretical basis for adjustment of radiation dose for patients with spinal tumor and internal fixation. based on a common method of internal fixation after resection of spinal tumor, different models of spinal internal fixation were constructed using the lumbar vertebra of fresh domestic pigs and titanium alloy as the internal fixation system. variations in radia ... | 2015 | 26219011 |
| a novel porcine bocavirus harbors a variant np gene. | porcine bocavirus is classified within the genus bocaparvovirus, family parvoviridae. unlike other parvoviruses, the members of genus bocaparvovirus (bocaparvoviruses) encode an additional open reading frame (np1). many strains of pbovs have been identified in domestic pigs and recognized as a potential emerging pathogen causing respiratory and gastrointestinal disease. | 2015 | 26217547 |
| effect of body position on ventilation distribution during peep titration in a porcine model of acute lung injury using advanced respiratory monitoring and electrical impedance tomography. | lung failure after acute lung injury remains a challenge in different clinical settings. various interventions for restoration of gas exchange have been investigated. recruitment of collapsed alveoli by positive end expiratory pressure (peep) titration and optimization of ventilation-perfusion ratio by prone positioning have been extensively described in animal and clinical trials. this animal study was conducted to investigate the effects of peep and positioning by means of advanced respiratory ... | 2015 | 26215805 |
| safety and efficacy of new sirolimus-eluting stent models in a preclinical study. | initial preclinical studies are required during the process of improving polymers, platforms, and drug-eluting systems for new coronary stent designs. our objective was to analyze the efficacy and safety of new drug-eluting stent models compared with a conventional stent and commercialized drug-eluting stents in an experimental model with healthy porcine coronary arteries. | 2015 | 26206247 |
| topical antibiotic ointment versus silver-containing foam dressing for second-degree burns in swine. | second-degree burns are very common but their management is controversial. these burns may be treated with either topical antimicrobial agents or advanced occlusive dressings; however, there is no established treatment comparator for preclinical studies. this study was designed to determine which of two commonly used comparator therapies (a silver-containing advanced dressing or a topical antibiotic ointment) resulted in faster reepithelialization and less scarring. the hypothesis was that secon ... | 2015 | 26202791 |
| allogenic ipsc-derived rpe cell transplants induce immune response in pigs: a pilot study. | stem cell strategies focused on replacement of rpe cells for the treatment of geographic atrophy are under intense investigation. although the eye has long been considered immune privileged, there is limited information about the immune response to transplanted cells in the subretinal space of large animals. the purpose of this study was to evaluate the survival of allogenic induced pluripotent stem cell-derived rpe cells (ipsc-rpe) delivered to the subretinal space of the pig as well as determi ... | 2015 | 26138532 |
| [18f]fdg accumulation in early coronary atherosclerotic lesions in pigs. | inflammation is an important contributor to atherosclerosis progression. a glucose analogue 18f-fluorodeoxyglucose ([18f]fdg) has been used to detect atherosclerotic inflammation. however, it is not known to what extent [18f]fdg is taken up in different stages of atherosclerosis. we aimed to study the uptake of [18f]fdg to various stages of coronary plaques in a pig model. | 2015 | 26120829 |
| electrophysiological, haemodynamic, and mitochondrial alterations induced by levobupivacaine during myocardial ischemia in a pig model: protection by lipid emulsions? | accidental intravascular or high-dose injection of local anesthetics (la) can result in serious, potentially life-threatening complications. indeed, adequate supportive measures and the administration of lipid emulsions are required in such complications. the study's objectives were threefold: (i) evaluate the myocardial toxicity of levobupivacaine when administered intravenously; (ii) investigate levobupivacaine toxicity on cardiomyocytes mitochondrial functions and cellular structure; (iii) as ... | 2015 | 26118736 |
| poly-d-l-lactic acid membranes for bone regeneration. | to histologically and histomorphometrically evaluate the biocompatibility and the biological properties of perforated and nonperforated poly-d-l-lactic acid (pdlla) resorbable membranes, using a model of calvarial monocortical bone defects in domestic pigs. | 2015 | 26114511 |
| vasopressin infusion with small-volume fluid resuscitation during hemorrhagic shock promotes hemodynamic stability and survival in swine. | current management of hemorrhagic shock (hs) in the battlefield and civilian settings favors small-volume fluid resuscitation before controlling the source of bleeding. we investigated in a swine model of hs the effects of vasopressin infusion along with small-volume fluid resuscitation; with erythropoietin (epo) and hs severity as additional factors. | 2015 | 26107942 |
| the interactive effect of the degradation of cotton clothing and decomposition fluid production associated with decaying remains. | textiles are a commonly encountered source of evidence in forensic cases. in the past, most research has been focused on how textiles affect the decomposition process while little attention has been paid to how the decomposition products interact with the textiles. while some studies have shown that the presence of remains will have an effect on the degradation of clothing associated with a decaying body, very little work has been carried out on the specific mechanisms that prevent or delay text ... | 2015 | 26074165 |
| comparison of ketamine-dexmedetomidine-methadone and tiletamine-zolazepam-methadone combinations for short-term anaesthesia in domestic pigs. | cardiorespiratory effects, quality of induction, depth of anaesthesia and quality of recovery were compared in pigs anaesthetised with 8 mg/kg ketamine, 20 µg/kg dexmedetomidine and 0.2 mg/kg methadone (kdm, n = 18) or 8 mg/kg tiletamine-zolazepam and 0.2 mg/kg methadone (tzm, n = 9). anaesthesia with kdm was partially reversed in nine animals with 0.2 mg/kg atipamezole (kdmat). sedation was observed earlier in the tzm group (47.2 ± 25.3 s) than the kdm group (91.5 ± 37.4 s). sternal and lateral ... | 2015 | 26070949 |
| introducer development for coronary sinus access from parasternal mediastinotomy. | right parasternal mediastinotomy with right atriotomy has been used clinically for pacemaker insertion. a similar approach might facilitate access to the coronary sinus for biventricular pacing and other manipulations when more conventional approaches are not feasible. the primary barrier to this is lack of appropriate introducers and techniques. | 2015 | 26181586 |
| experimental study of temperature-sensitive chitosan/β-glycerophosphate embolic material in embolizing the basicranial rete mirabile in swines. | the aim of the present study was to evaluate the feasibility of the non-adhesive temperature-sensitive liquid embolic material, chitosan/β-glycerophosphate (c/gp), in embolizing the basicranial rete mirabile (rem) in a swine model of cerebral arteriovenous malformation (cavm). a total of 24 domestic swines were used as the experimental animals, among which 12 pigs underwent direct embolization of one side of the rem, while the other 12 pigs underwent embolization of the bilateral rem following a ... | 2015 | 26170955 |
| intermediate veins in swine (sus scrofa domestica) kidney: authors' own anatomical classification. | the use of domestic swine as an experimental animal is increasing steadily. swine organs are the best animal model for urological experiments. the aim of the study was to evaluate the course and size of intermediate veins in a swine kidney. the research results were compared with the results obtained from studies on venous vascularisation of human kidneys. the knowledge of the above-mentioned vessels is important both in human and veterinary medicine and will enable researchers to compare and no ... | 2015 | 26050812 |
| vegf-c and vegf-c156s in the pro-lymphangiogenic growth factor therapy of lymphedema: a large animal study. | vegf-c156s, a lymphangiogenesis-specific form of vascular endothelial growth factor c (vegf-c), has been considered as a promising candidate for the experimental pro-lymphangiogenic treatment, as it lacks potential angiogenic effects. as a precursor to future clinical trials, the therapeutic efficacy and blood vascular side effects of vegf-c and vegf-c156s were compared in a large animal model of secondary lymphedema. combination of lymphatic growth factor treatment and autologous lymph node tra ... | 2015 | 26018927 |
| the synergy biodegradable polymer everolimus eluting coronary stent: porcine vascular compatibility and polymer safety study. | synergy is a novel platinum chromium alloy stent that delivers abluminal everolimus from an ultrathin poly-lactide-co-glycide (plga) biodegradable polymer. this study evaluated the in vivo degradation of the polymer coating, everolimus release time course, and vascular compatibility of the synergy stent. | 2015 | 26009986 |
| dose-dependent vascular response following delivery of sirolimus via fast releasing, biodegradable polymer stent matrix: an experimental study in the porcine coronary model of restenosis. | fast releasing, rapamycin-eluting stents, although safe, showed inferior results with regard to inhibition of restenosis. | 2015 | 25985728 |