Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| using genome-wide measures of coancestry to maintain diversity and fitness in endangered and domestic pig populations. | conservation and breeding programs aim at maintaining the most diversity, thereby avoiding deleterious effects of inbreeding while maintaining enough variation from which traits of interest can be selected. theoretically, the most diversity is maintained using optimal contributions based on many markers to calculate coancestries, but this can decrease fitness by maintaining linked deleterious variants. the heterogeneous patterns of coancestry displayed in pigs make them an excellent model to tes ... | 2015 | 26063737 |
| comparison of ketamine-dexmedetomidine-methadone and tiletamine-zolazepam-methadone combinations for short-term anaesthesia in domestic pigs. | cardiorespiratory effects, quality of induction, depth of anaesthesia and quality of recovery were compared in pigs anaesthetised with 8 mg/kg ketamine, 20 µg/kg dexmedetomidine and 0.2 mg/kg methadone (kdm, n = 18) or 8 mg/kg tiletamine-zolazepam and 0.2 mg/kg methadone (tzm, n = 9). anaesthesia with kdm was partially reversed in nine animals with 0.2 mg/kg atipamezole (kdmat). sedation was observed earlier in the tzm group (47.2 ± 25.3 s) than the kdm group (91.5 ± 37.4 s). sternal and lateral ... | 2015 | 26070949 |
| the interactive effect of the degradation of cotton clothing and decomposition fluid production associated with decaying remains. | textiles are a commonly encountered source of evidence in forensic cases. in the past, most research has been focused on how textiles affect the decomposition process while little attention has been paid to how the decomposition products interact with the textiles. while some studies have shown that the presence of remains will have an effect on the degradation of clothing associated with a decaying body, very little work has been carried out on the specific mechanisms that prevent or delay text ... | 2015 | 26074165 |
| xromm analysis of tooth occlusion and temporomandibular joint kinematics during feeding in juvenile miniature pigs. | like humans, domestic pigs are omnivorous and thus are a common model for human masticatory function. prior attempts to characterize food-tooth interactions and jaw movements associated with mastication have been limited to aspects of the oral apparatus that are visible externally (with videography) and/or to 2d movements of oral structures (with monoplanar videofluoroscopy). we used xromm, a 3d technique that combines ct-based morphology with biplanar videofluoroscopy, to quantify mandibular ki ... | 2015 | 26089531 |
| hepatitis e virus of subtype 3a in a pig farm, south-eastern france. | hepatitis e virus (hev) has emerged during the past decade as a causative agent of autochthonous hepatitis and is a clinical concern in western developed countries. it has been increasingly recognized that pigs are a major reservoir of hev of genotypes 3 and 4 worldwide and pig-derived food items represent a potential source of infections by these viruses in humans. hepatitis e virus rna testing was performed here on faeces from rectal swabs sampled in 2012 from 50 3-month-old farm pigs from the ... | 2015 | 26102074 |
| vasopressin infusion with small-volume fluid resuscitation during hemorrhagic shock promotes hemodynamic stability and survival in swine. | current management of hemorrhagic shock (hs) in the battlefield and civilian settings favors small-volume fluid resuscitation before controlling the source of bleeding. we investigated in a swine model of hs the effects of vasopressin infusion along with small-volume fluid resuscitation; with erythropoietin (epo) and hs severity as additional factors. | 2015 | 26107942 |
| poly-d-l-lactic acid membranes for bone regeneration. | to histologically and histomorphometrically evaluate the biocompatibility and the biological properties of perforated and nonperforated poly-d-l-lactic acid (pdlla) resorbable membranes, using a model of calvarial monocortical bone defects in domestic pigs. | 2015 | 26114511 |
| isolation and characterization of a novel rhabdovirus from a wild boar (sus scrofa) in japan. | a novel rhabdovirus was isolated from the serum of a healthy japanese wild boar (sus scrofa leucomystax) and identified using the rapid determination system for viral nucleic acid sequences (rdv), next-generation sequencing, and electron microscopy. the virus was tentatively named wild boar rhabdovirus 1 (wbrv1). phylogenetic analysis of the entire genome sequence indicated that wbrv1 is closely related to tupaia rhabdovirus (trv), which was isolated from cultured cells of hepatocellular carcino ... | 2015 | 26116524 |
| chinese border disease virus strain jsls12-01 infects piglets and down-regulates the antibody responses of classical swine fever virus c strain vaccination. | during 2012 and 2013, several border disease virus (bdv) strains were identified from chinese goat and sheep herds. at the same time, pigs from the same areas were found to be seropositive to bdv by elisa, without showing clinical signs (unpublished data). to examine the susceptibility of pigs to the chinese bdv strains, bdv isolate jsls12-01, isolated from naturally infected sheep, was used to infect pigs. antibody responses, viremia, clinical signs and pathological changes of the infected anim ... | 2015 | 26117151 |
| electrophysiological, haemodynamic, and mitochondrial alterations induced by levobupivacaine during myocardial ischemia in a pig model: protection by lipid emulsions? | accidental intravascular or high-dose injection of local anesthetics (la) can result in serious, potentially life-threatening complications. indeed, adequate supportive measures and the administration of lipid emulsions are required in such complications. the study's objectives were threefold: (i) evaluate the myocardial toxicity of levobupivacaine when administered intravenously; (ii) investigate levobupivacaine toxicity on cardiomyocytes mitochondrial functions and cellular structure; (iii) as ... | 2015 | 26118736 |
| chemical and metabolic profiling of si-ni decoction analogous formulae by high performance liquid chromatography-mass spectrometry. | along with an indispensable role in healthcare system of china for centuries, traditional chinese medicine (tcm) shows increasing usages as complementary therapy in western countries. to improve our understanding on their therapeutic effects, it's critical to unveil chemical compositions of tcm formula, the predominant form of therapy in tcm. however, intrinsic chemical complexity makes it a challenging task to perform analysis on each individual tcm formula even with most current state-of-art a ... | 2015 | 26118924 |
| [18f]fdg accumulation in early coronary atherosclerotic lesions in pigs. | inflammation is an important contributor to atherosclerosis progression. a glucose analogue 18f-fluorodeoxyglucose ([18f]fdg) has been used to detect atherosclerotic inflammation. however, it is not known to what extent [18f]fdg is taken up in different stages of atherosclerosis. we aimed to study the uptake of [18f]fdg to various stages of coronary plaques in a pig model. | 2015 | 26120829 |
| comparisons and uncertainty in fat and adipose tissue estimation techniques: the northern elephant seal as a case study. | fat mass and body condition are important metrics in bioenergetics and physiological studies. they can also link foraging success with demographic rates, making them key components of models that predict population-level outcomes of environmental change. therefore, it is important to incorporate uncertainty in physiological indicators if results will lead to species management decisions. maternal fat mass in elephant seals (mirounga spp) can predict reproductive rate and pup survival, but no one ... | 2015 | 26121044 |
| the porcine tspy gene is tricopy but not a copy number variant. | the testis-specific protein y-encoded (tspy) gene is situated on the mammalian y-chromosome and exhibits some remarkable biological characteristics. it has the highest known copy number (cn) of all protein coding genes in the human and bovine genomes (up to 74 and 200, respectively) and also shows high individual variability. although the biological function of tspy has not yet been elucidated, its specific expression in the testis and several identified binding domains within the protein sugges ... | 2015 | 26133983 |
| allogenic ipsc-derived rpe cell transplants induce immune response in pigs: a pilot study. | stem cell strategies focused on replacement of rpe cells for the treatment of geographic atrophy are under intense investigation. although the eye has long been considered immune privileged, there is limited information about the immune response to transplanted cells in the subretinal space of large animals. the purpose of this study was to evaluate the survival of allogenic induced pluripotent stem cell-derived rpe cells (ipsc-rpe) delivered to the subretinal space of the pig as well as determi ... | 2015 | 26138532 |
| cross-reactivity between aeroallergens and food allergens. | in patients with respiratory allergy, cross-reactivity between aeroallergens and foods may induce food allergy, symptoms ranging from oral allergy syndrome to severe anaphylaxis. clinical entities due to ige sensitization to cross-reactive aeroallergen and food allergen components are described for many sources of plant origin (pollen-food syndromes and associations, such as birch-apple, cypress-peach and celery-mugwort-spice syndromes, and mugwort-peach, mugwort-chamomile, mugwort-mustard, ragw ... | 2015 | 26140270 |
| hepatitis e virus in domestic pigs, wild boars, pig farm workers, and hunters in estonia. | while hepatitis e is a growing health concern in europe, epidemiological data on hepatitis e virus (hev) in estonia are scarce. along with imported hev infections, autochthonous cases are reported from european countries. both domestic and wild animals can be a source of human cases of this zoonosis. here, we investigated the presence of anti-hev antibodies and hev rna in domestic pigs and wild boars, as well as in pig farm workers and hunters in estonia. anti-hev antibodies were detected in 234 ... | 2015 | 26141050 |
| optical coherence tomography of pulmonary arterial walls in humans and pigs (sus scrofa domesticus). | pulmonary arterial hypertension (pah) is a devastating disorder characterized by progressive elevation of the pulmonary pressures that, in the absence of therapy, results in chronic right-heart failure and premature death. the vascular pathology of pah is characterized by progressive loss of small (diameter, less than 50 μm) peripheral pulmonary arteries along with abnormal medial thickening, neointimal formation, and intraluminal narrowing of the remaining pulmonary arteries. vascular pathology ... | 0 | 26141446 |
| validation of respiratory inductance plethysmography for measuring tidal volume in swine. | measuring tidal volume (vt) in nonintubated swine or swine with leaking breathing circuits is challenging. the aim of this study was to validate respiratory inductance plethysmography (rip) for measuring vt in swine that are comparable in size to adult humans. to determine calibration curves, vt and rip readings were obtained from anesthetized swine (n = 8; weight, 46-50 kg) during positive-pressure (mechanical) ventilation and spontaneous breathing. for positive-pressure ventilation, 6 pigs wer ... | 0 | 26141447 |
| alpha-crystallin-derived peptides as therapeutic chaperones. | the demonstration of chaperone-like activity in peptides (mini-chaperones) derived from α-crystallin's chaperone region has generated significant interest in exploring the therapeutic potential of peptide chaperones in diseases of protein aggregation. recent studies in experimental animals show that mini-chaperones could reach intended targets and alter the disease phenotype. although mini-chaperones show potential benefits against protein aggregation diseases, they do tend to form aggregates on ... | 2015 | 26141743 |
| submerged flapless technique vs. conventional flap approach for implant placement: experimental domestic pig study with 12-month follow-up. | the aim of our study was to compare osseointegration and peri-implant crestal bone resorption in submerged flapless and conventional flap surgery over a 12-month follow-up. | 2016 | 26147852 |
| genome wide distributions and functional characterization of copy number variations between chinese and western pigs. | copy number variations (cnvs) refer to large insertions, deletions and duplications in the genomic structure ranging from one thousand to several million bases in size. since the development of next generation sequencing technology, several methods have been well built for detection of copy number variations with high credibility and accuracy. evidence has shown that cnv occurring in gene region could lead to phenotypic changes due to the alteration in gene structure and dosage. however, it stil ... | 2015 | 26154170 |
| evaluation of a commercial elisa for the detection of antibodies to sarcoptes scabiei in wild boar (sus scrofa). | sarcoptic mange occurs in free-ranging wild boar (sus scrofa) but has been poorly described in this species. we evaluated the performance of a commercial indirect enzyme-linked immunosorbent assay (elisa) for serodiagnosis of sarcoptic mange in domestic swine when applied to wild boar sera. we tested 96 sera from wild boar in populations without mange history ("truly noninfected") collected in switzerland between december 2012 and february 2014, and 141 sera from free-ranging wild boar presentin ... | 2015 | 26161723 |
| new porcine epidemic diarrhoea virus variant with a large deletion in the spike gene identified in domestic pigs. | from october 2013 to date, approximately 1,000 outbreaks of porcine epidemic diarrhoea virus (pedv) have occurred in japan. porcine epidemic diarrhoea with non-lethal effects in piglets was identified in tottori prefecture in october 2014. complete genome analysis revealed that the causative pathogen, tottori2, is a new pedv variant with a large (582 nt) deletion in the spike gene. phylogenetic analysis indicated that the tottori2 pedv strain might have been derived from the current pedv strains ... | 2015 | 26162305 |
| radionuclide therapy of unresectable tumors with avidinox and (90)y-biotindota: tongue cancer paradigm. | local treatment of unresectable tumors is challenging, particularly with radioactivity. current practice relies on external beam irradiation or on a variety of medical devices for brachytherapy. both approaches proved useful in controlling tumor growth, but are characterized by poor compliance of the patient, significant side-effects, high costs, and technological complexity, which hamper widespread use. the authors recently described a novel form of radionuclide therapy based on the oxidized fo ... | 2015 | 26167947 |
| experimental study of temperature-sensitive chitosan/β-glycerophosphate embolic material in embolizing the basicranial rete mirabile in swines. | the aim of the present study was to evaluate the feasibility of the non-adhesive temperature-sensitive liquid embolic material, chitosan/β-glycerophosphate (c/gp), in embolizing the basicranial rete mirabile (rem) in a swine model of cerebral arteriovenous malformation (cavm). a total of 24 domestic swines were used as the experimental animals, among which 12 pigs underwent direct embolization of one side of the rem, while the other 12 pigs underwent embolization of the bilateral rem following a ... | 2015 | 26170955 |
| increased b and t cell responses in m. bovis bacille calmette-guérin vaccinated pigs co-immunized with plasmid dna encoding a prototype tuberculosis antigen. | the only tuberculosis vaccine currently available, bacille calmette-guérin (bcg) is a poor inducer of cd8(+) t cells, which are particularly important for the control of latent tuberculosis and protection against reactivation. as the induction of strong cd8(+) t cell responses is a hallmark of dna vaccines, a combination of bcg with plasmid dna encoding a prototype tb antigen (ag85a) was tested. as an alternative animal model, pigs were primed with bcg mixed with empty vector or codon-optimized ... | 2015 | 26172261 |
| pbmc transcriptomic responses to primary and secondary vaccination differ due to divergent lean growth and antibody titers in a pig model. | the genetic relationship between immune responsiveness and performance is not well understood, but a major topic of the evolution of resource allocation and of relevance in human medicine and livestock breeding, for instance. this study aims to show differences of transcript abundance changes during the time intervals before and after two tetanus toxoid (tt) vaccinations in domestic pigs differing in lean growth (lg) and anti-tt-antibody titers (ab) parameters of performance and immunocompetence ... | 2015 | 26175500 |
| evaluation of an erns-based enzyme-linked immunosorbent assay to distinguish classical swine fever virus-infected pigs from pigs vaccinated with cp7_e2alf. | infections with classical swine fever virus (csfv) are a major economic threat to pig production. to combat csf outbreaks and to maintain trade, new marker vaccines were developed that allow differentiation of infected from vaccinated animals (diva principle). the chimeric pestivirus cp7_e2alf was shown to be safe and efficacious. its diva strategy is based on the detection of csfv e(rns)-specific antibodies that are only developed on infection. however, for the new marker vaccine to be consider ... | 2015 | 26179095 |
| introducer development for coronary sinus access from parasternal mediastinotomy. | right parasternal mediastinotomy with right atriotomy has been used clinically for pacemaker insertion. a similar approach might facilitate access to the coronary sinus for biventricular pacing and other manipulations when more conventional approaches are not feasible. the primary barrier to this is lack of appropriate introducers and techniques. | 2015 | 26181586 |
| molecular evidence of ebola reston virus infection in philippine bats. | in 2008-09, evidence of reston ebolavirus (restv) infection was found in domestic pigs and pig workers in the philippines. with species of bats having been shown to be the cryptic reservoir of filoviruses elsewhere, the philippine government, in conjunction with the food and agriculture organization of the united nations, assembled a multi-disciplinary and multi-institutional team to investigate philippine bats as the possible reservoir of restv. | 2015 | 26184657 |
| the red fox (vulpes vulpes) plays a minor role in the epidemiology of the domestic cycle of trichinella in romania. | nematode worms of the genus trichinella are zoonotic parasites with a worldwide distribution. the majority of the biomass of these nematodes circulates among wildlife, but when humans fail in the proper management of domestic animals and wildlife, trichinella infections are transmitted from the sylvatic to the domestic environment. such failures occur in romania, where a high prevalence of trichinella spiralis has been detected in domestic pigs. the aim of the present study was to provide data a ... | 2015 | 26185060 |
| experimental evaluation of faecal escherichia coli and hepatitis e virus as biological indicators of contacts between domestic pigs and eurasian wild boar. | domestic pigs and eurasian wild boar (sus scrofa) share several important viral and bacterial pathogens. therefore, direct and indirect contacts between domestic pigs and wild boar present a risk of pathogen spillover and can lead to long-term perpetuation of infection. biological indicators could be a powerful tool to understand and characterize contacts between wild boar and domestic pigs. here, faecal escherichia coli and hepatitis e virus (hev) were explored as potential biological indicator ... | 2017 | 26190581 |
| statistical exploration of local transmission routes for african swine fever in pigs in the russian federation, 2007-2014. | african swine fever (asf) is a devastating viral disease of swine that is present in both pigs and wild boar in the western part of the russian federation and the eastern part of the european union. it represents a significant threat for the european pig production industry as neither treatment nor vaccine is available. this study analysed the spatial and spatio-temporal distributions of asf cases that were reported in domestic pigs and wild boar for assessing the likelihood of wild boar-to-dome ... | 2017 | 26192820 |
| serologic and molecular survey for hepatitis e virus in wild boar (sus scrofa) in central italy. | the aim of this study was to further investigate the role of wild boar (sus scrofa) as a reservoir for hepatitis e virus (hev). sixty-four blood and faecal samples collected from wild boar hunted in central italy in 2011-2012 were examined by indirect enzyme-linked immunosorbent assay and rt-pcr analysis. positive rt-pcr samples were further examined by nucleotide sequence determination and subsequent phylogenetic analysis. thirty-six sera (56.2%) were positive for hev-specific antibodies, and s ... | 2015 | 26199731 |
| topical antibiotic ointment versus silver-containing foam dressing for second-degree burns in swine. | second-degree burns are very common but their management is controversial. these burns may be treated with either topical antimicrobial agents or advanced occlusive dressings; however, there is no established treatment comparator for preclinical studies. this study was designed to determine which of two commonly used comparator therapies (a silver-containing advanced dressing or a topical antibiotic ointment) resulted in faster reepithelialization and less scarring. the hypothesis was that secon ... | 2015 | 26202791 |
| the first report of trichinella pseudospiralis presence in domestic swine and t. britovi in wild boar in bosnia and herzegovina. | the balkans is endemic for nematodes of the genus trichinella in both domestic and wild animals. the high prevalence of these zoonotic pathogens in animals linked with the food habits to consume raw meat and meat derived products resulted in a very high prevalence of trichinellosis in humans living in this european region. in spite of numerous epidemiological investigations carried out in this region, very few information is available on the trichinella species circulating in bosnia and herzegov ... | 2015 | 26204186 |
| safety and efficacy of new sirolimus-eluting stent models in a preclinical study. | initial preclinical studies are required during the process of improving polymers, platforms, and drug-eluting systems for new coronary stent designs. our objective was to analyze the efficacy and safety of new drug-eluting stent models compared with a conventional stent and commercialized drug-eluting stents in an experimental model with healthy porcine coronary arteries. | 2015 | 26206247 |
| 1ppab5. acoustic radiation force to reposition kidney stones. | our group has introduced transcutaneous ultrasound to move kidney stones in order to expel small stones or relocate an obstructing stone to a nonobstructing location. human stones and metalized beads (2-8 mm) were implanted ureteroscopically in kidneys of eight domestic swine. ultrasonic propulsion was performed using a diagnostic imaging transducer and a verasonics ultrasound platform. stone propulsion was visualized using fluoroscopy, ultrasound, and the ureteroscope. successful stone movement ... | 2017 | 26207161 |
| epitope recognition in the human-pig comparison model on fixed and embedded material. | the conditions and the specificity by which an antibody binds to its target protein in routinely fixed and embedded tissues are unknown. direct methods, such as staining in a knock-out animal or in vitro peptide scanning of the epitope, are costly and impractical. we aimed to elucidate antibody specificity and binding conditions using tissue staining and public genomic and immunological databases by comparing human and pig-the farmed mammal evolutionarily closest to humans besides apes. we used ... | 2015 | 26209082 |
| effect of body position on ventilation distribution during peep titration in a porcine model of acute lung injury using advanced respiratory monitoring and electrical impedance tomography. | lung failure after acute lung injury remains a challenge in different clinical settings. various interventions for restoration of gas exchange have been investigated. recruitment of collapsed alveoli by positive end expiratory pressure (peep) titration and optimization of ventilation-perfusion ratio by prone positioning have been extensively described in animal and clinical trials. this animal study was conducted to investigate the effects of peep and positioning by means of advanced respiratory ... | 2015 | 26215805 |
| a novel porcine bocavirus harbors a variant np gene. | porcine bocavirus is classified within the genus bocaparvovirus, family parvoviridae. unlike other parvoviruses, the members of genus bocaparvovirus (bocaparvoviruses) encode an additional open reading frame (np1). many strains of pbovs have been identified in domestic pigs and recognized as a potential emerging pathogen causing respiratory and gastrointestinal disease. | 2015 | 26217547 |
| using bacterial and necrophagous insect dynamics for post-mortem interval estimation during cold season: novel case study in romania. | considering the biogeographical characteristics of forensic entomology, and the recent development of forensic microbiology as a complementary approach for post-mortem interval estimation, the current study focused on characterizing the succession of necrophagous insect species and bacterial communities inhabiting the rectum and mouth cavities of swine (sus scrofa domesticus) carcasses during a cold season outdoor experiment in an urban natural environment of bucharest, romania. we monitored the ... | 2015 | 26217916 |
| lymphatic territories (lymphosomes) in swine: an animal model for future lymphatic research. | the swine is a common preclinical large-animal model for medical research because of the resemblance of its tissue structures to those of humans. however, the lymphatic system in swine is poorly understood. the authors investigated the lymphatic system and defined territories (lymphosomes) in swine using the microinjection technique. | 2015 | 26218378 |
| influence of internal fixation systems on radiation therapy for spinal tumor. | in this study, the influence of internal fixation systems on radiation therapy for spinal tumor was investigated in order to derive a theoretical basis for adjustment of radiation dose for patients with spinal tumor and internal fixation. based on a common method of internal fixation after resection of spinal tumor, different models of spinal internal fixation were constructed using the lumbar vertebra of fresh domestic pigs and titanium alloy as the internal fixation system. variations in radia ... | 2015 | 26219011 |
| the evolution of stomach acidity and its relevance to the human microbiome. | gastric acidity is likely a key factor shaping the diversity and composition of microbial communities found in the vertebrate gut. we conducted a systematic review to test the hypothesis that a key role of the vertebrate stomach is to maintain the gut microbial community by filtering out novel microbial taxa before they pass into the intestines. we propose that species feeding either on carrion or on organisms that are close phylogenetic relatives should require the most restrictive filter (meas ... | 2015 | 26222383 |
| quantitative risk assessment of human trichinellosis caused by consumption of pork meat sausages in argentina. | in argentina, there are three known species of genus trichinella; however, trichinella spiralis is most commonly associated with domestic pigs and it is recognized as the main cause of human trichinellosis by the consumption of products made with raw or insufficiently cooked pork meat. in some areas of argentina, this disease is endemic and it is thus necessary to develop a more effective programme of prevention and control. here, we developed a quantitative risk assessment of human trichinellos ... | 2016 | 26227185 |
| mechanistic insights into glucan phosphatase activity against polyglucan substrates. | glucan phosphatases are central to the regulation of starch and glycogen metabolism. plants contain two known glucan phosphatases, starch excess4 (sex4) and like sex four2 (lsf2), which dephosphorylate starch. starch is water-insoluble and reversible phosphorylation solubilizes its outer surface allowing processive degradation. vertebrates contain a single known glucan phosphatase, laforin, that dephosphorylates glycogen. in the absence of laforin, water-soluble glycogen becomes insoluble, leadi ... | 2015 | 26231210 |
| segmental and age differences in the elastin network, collagen, and smooth muscle phenotype in the tunica media of the porcine aorta. | the porcine aorta is often used in studies on morphology, pathology, transplantation surgery, vascular and endovascular surgery, and biomechanics of the large arteries. using quantitative histology and stereology, we estimated the area fraction of elastin, collagen, alpha-smooth muscle actin, vimentin, and desmin within the tunica media in 123 tissue samples collected from five segments (thoracic ascending aorta; aortic arch; thoracic descending aorta; suprarenal abdominal aorta; and infrarenal ... | 2015 | 26232584 |
| characterization of a porcine model for associating liver partition and portal vein ligation for a staged hepatectomy. | publications using the alpps (associating liver partition and portal vein ligation for a staged hepatectomy) procedure have demonstrated a future liver remnant growth of 40-160% in only 6-9 days. the present study aimed to develop and describe the first large animal model of alpps that can be used for future studies. | 2015 | 26234167 |
| new insights into the molecular epidemiology of trichinella infection in domestic pigs, wild boars, and bears in romania. | trichinellosis is a food-borne zoonosis caused by the parasitic nematode trichinella, characterized by an extremely wide host range and geographical distribution. in romania, it is recognized as one of the most serious zoonotic diseases. a cross-sectional study, covering all regions of romania, was conducted in 2014 to investigate and update the prevalence of trichinella infection among domestic pigs, wild boars, and bears. additional, molecular identification of trichinella species circulating ... | 2015 | 26238657 |
| a pharmacologic activator of endothelial kca channels increases systemic conductance and reduces arterial pressure in an anesthetized pig model. | ska-31, an activator of endothelial kca2.3 and kca3.1 channels, reduces systemic blood pressure in mice and dogs, however, its effects in larger mammals are not well known. we therefore examined the hemodynamic effects of ska-31, along with sodium nitroprusside (snp), in anesthetized, juvenile male domestic pigs. experimentally, continuous measurements of left ventricular (lv), aortic and inferior vena cava (ivc) pressures, along with flows in the ascending aorta, carotid artery, left anterior d ... | 2016 | 26239885 |
| a triple culture model of the blood-brain barrier using porcine brain endothelial cells, astrocytes and pericytes. | in vitro blood-brain barrier (bbb) models based on primary brain endothelial cells (becs) cultured as monoculture or in co-culture with primary astrocytes and pericytes are useful for studying many properties of the bbb. the becs retain their expression of tight junction proteins and efflux transporters leading to high trans-endothelial electric resistance (teer) and low passive paracellular permeability. the becs, astrocytes and pericytes are often isolated from small rodents. larger species as ... | 2015 | 26241648 |
| genomic diversity and differentiation of a managed island wild boar population. | the evolution of island populations in natural systems is driven by local adaptation and genetic drift. however, evolutionary pathways may be altered by humans in several ways. the wild boar (wb) (sus scrofa) is an iconic game species occurring in several islands, where it has been strongly managed since prehistoric times. we examined genomic diversity at 49 803 single-nucleotide polymorphisms in 99 sardinian wbs and compared them with 196 wild specimens from mainland europe and 105 domestic pig ... | 2015 | 26243137 |
| disease risks associated with free-ranging wild boar in saskatchewan. | this study investigated the disease status of saskatchewan's feral wild boar population. whole carcasses, tissue samples, and/or serum from 81 hunter-killed boars from saskatchewan were submitted to the canadian wildlife health cooperative (cwhc) between 2009 and 2014. serological tests were negative for prrs, h1n1, and h3n2 swine influenza, pcv-2, and tge/prcv in 22/22 boars and for toxoplasma gondii and mycoplasma hyopneumoniae in 20/20 boars. of 20 boars whose sera were tested 20 were positiv ... | 0 | 26246630 |
| a large-scale study of the trichinella genus in the golden jackal (canis aureus) population in serbia. | over the last decades the golden jackal (canis aureus) has significantly expanded its range throughout southeast and central europe, and the balkan peninsula is considered to be a core area of the species distribution in this part of the range. due to its increasing number, ability of long distance movement through a wide range of landscapes and opportunistic feeding habits, the golden jackal may represent an important reservoir and transmitter of a variety of zoonotic agents, including parasite ... | 2015 | 26260409 |
| a serosurvey for brucellosis in wild boar (sus scrofa) in sardinia, italy. | porcine brucellosis is a zoonotic disease caused by brucella suis and hosted by pigs (sus scrofa). both domestic pigs and wild boars are affected. we measured the prevalence of antibody to brucella spp. in wild boars in sardinia, italy. during 1 november 2009 to 31 january 2010, we collected 570 serum samples from legally hunted wild boars and tested them using a commercial competitive enzyme-linked immunosorbent assay. sex and age class of the sampled wild boars were also recorded. thirty-five ... | 2015 | 26267463 |
| increasing venoarterial extracorporeal membrane oxygenation flow negatively affects left ventricular performance in a porcine model of cardiogenic shock. | the aim of this study was to assess the relationship between extracorporeal blood flow (ebf) and left ventricular (lv) performance during venoarterial extracorporeal membrane oxygenation (va ecmo) therapy. | 2015 | 26275717 |
| effects of dietary protein/energy ratio on growth performance, carcass trait, meat quality, and plasma metabolites in pigs of different genotypes. | the protein/energy ratio is important for the production performance and utilization of available feed resources by animals. increased protein consumption by mammals leads to elevated feed costs and increased nitrogen release into the environment. this study aimed to evaluate the effects of dietary protein/energy ratio on the growth performance, carcass traits, meat quality, and plasma metabolites of pigs of different genotypes. | 2015 | 26279834 |
| clonal transmission of a colistin-resistant escherichia coli from a domesticated pig to a human in laos. | 2015 | 26283671 | |
| hypoxanthine-guanine phosphoribosyltransferase and inosine 5'-monophosphate dehydrogenase activities in three mammalian species: aquatic (mirounga angustirostris), semi-aquatic (lontra longicaudis annectens) and terrestrial (sus scrofa). | aquatic and semiaquatic mammals have the capacity of breath hold (apnea) diving. northern elephant seals (mirounga angustirostris) have the ability to perform deep and long duration dives; during a routine dive, adults can hold their breath for 25 min. neotropical river otters (lontra longicaudis annectens) can hold their breath for about 30 s. such periods of apnea may result in reduced oxygen concentration (hypoxia) and reduced blood supply (ischemia) to tissues. production of adenosine 5'-tri ... | 2015 | 26283971 |
| exploring evidence of positive selection reveals genetic basis of meat quality traits in berkshire pigs through whole genome sequencing. | natural and artificial selection following domestication has led to the existence of more than a hundred pig breeds, as well as incredible variation in phenotypic traits. berkshire pigs are regarded as having superior meat quality compared to other breeds. as the meat production industry seeks selective breeding approaches to improve profitable traits such as meat quality, information about genetic determinants of these traits is in high demand. however, most of the studies have been performed u ... | 2015 | 26289667 |
| distribution of porcine endogenous retrovirus in different organs of the hybrid of a landrace and a jeju domestic pig in korea. | xenotransplantation offers a solution to the shortage of available organs for transplantation, and the pig represents an ideal source of such organs. however, porcine endogenous retrovirus (perv), whose genome is integrated in pigs, has been suggested to pose a potential risk of xenotransmission. expression of pervs in different organs of pigs was carefully measured at dna, mrna, and protein levels, providing information valuable for the application of pig organs in xenotransplantation. an analy ... | 2015 | 26293098 |
| dynamics of virus shedding and antibody responses in influenza a virus-infected feral swine. | given their free-ranging habits, feral swine could serve as reservoirs or spatially dynamic 'mixing vessels' for influenza a virus (iav). to better understand virus shedding patterns and antibody response dynamics in the context of iav surveillance amongst feral swine, we used iav of feral swine origin to perform infection experiments. the virus was highly infectious and transmissible in feral swine, and virus shedding patterns and antibody response dynamics were similar to those in domestic swi ... | 2015 | 26297148 |
| first molecular assessment of the african swine fever virus status of ornithodoros ticks from swaziland. | african swine fever (asf) is an economically significant haemorrhagic disease of domestic pigs. it is caused by the african swine fever virus (asfv), a deoxyribonucleic acid (dna)arbovirus. argasid ticks of the genus ornithodoros, which are widely distributed throughout southern africa, play a primary role in virus maintenance and spread within the endemic sylvatic cycle. the asf status of swaziland is unknown, but this land-locked country is surrounded by asf-positive countries, has a burgeonin ... | 2014 | 26304186 |
| porcine survival model to simulate acute upper gastrointestinal bleedings. | the existing animal models used for the simulation of acute gastrointestinal bleedings are usually non-survival models. we developed and evaluated a new porcine model (domestic pig, german landrace) in which the animal remains alive and survives the artificial bleeding without any cardiovascular impairment. this consists of a bleeding catheter which is implanted into the stomach, then subcutaneously tunnelled from the abdomen to the neck where it is exteriorized and fixed with sutures. using the ... | 2016 | 26306615 |
| one health, multiple challenges: the inter-species transmission of influenza a virus. | influenza a viruses are amongst the most challenging viruses that threaten both human and animal health. influenza a viruses are unique in many ways. firstly, they are unique in the diversity of host species that they infect. this includes waterfowl (the original reservoir), terrestrial and aquatic poultry, swine, humans, horses, dog, cats, whales, seals and several other mammalian species. secondly, they are unique in their capacity to evolve and adapt, following crossing the species barrier, i ... | 0 | 26309905 |
| assessing the potential role of pigs in the epidemiology of ebola virus in uganda. | uganda has experienced 4 ebola outbreaks since the discovery of the virus. recent epidemiological work has shown pigs are hosts for ebola viruses. due to their high reproduction rates, rapid weight gain, potential to provide quick financial returns and rising demand for pork, pig production in uganda has undergone massive expansion. the combination of pork sector growth supported by development programmes and ebola virus risk prompted a foresight exercise using desk, interview and spatial method ... | 2017 | 26310206 |
| spontaneous dilated cardiomyopathy and right-sided heart failure as a differential diagnosis for hepatosis dietetica in a production pig. | an experimentally naïve 37.7-kg yorkshire-crossbred gilt died unexpectedly 2 d after arrival. necropsy revealed severe dilated cardiomyopathy characterized grossly by markedly dilated ventricles and thinned ventricular walls and interventricular septum. histologically there was multifocal myofiber attenuation and patchy loss of myofiber cross striations. the liver contained submassive to massive, diffuse hepatic centrilobular hemorrhage and degeneration. these lesions supported a diagnosis of di ... | 0 | 26310462 |
| high prevalence of tritrichomonas foetus 'bovine genotype' in faecal samples from domestic pigs at a farm where bovine trichomonosis has not been reported for over 30 years. | bovine venereal trichomonosis caused by the flagellate tritrichomonas foetus is a notifiable disease in australia. while, t. foetus is pathogenic in both cattle and cats, it has long been established that the same t. foetus colonises the stomach, caecum and nasal cavity of pigs without apparent clinical significance. multi-locus genotyping grouped the non-pathogenic porcine t. foetus with the pathogenic 'bovine genotype', rather than with the 'feline genotype' t. foetus. bovine trichomonosis is ... | 2015 | 26315128 |
| domestication and cereal feeding developed domestic pig-type intestinal microbiota in animals of suidae. | intestinal microbiota are characterized by host-specific microorganisms, which have been selected through host-microbe interactions under phylogenetic evolution and transition of feeding behavior by the host. although many studies have focused on disease-related intestinal microbiota, the origin and evolution of host-specific intestinal microbiota have not been well elucidated. pig is the ideal mammal model to reveal the origin and evolution of host-specific intestinal microbiota because their d ... | 2016 | 26315488 |
| soil fungal community shift evaluation as a potential cadaver decomposition indicator. | fungi metabolise organic matter in situ and so alter both the bio-/physico-chemical properties and microbial community structure of the ecosystem. in particular, they are responsible reportedly for specific stages of decomposition. therefore, this study aimed to extend previous bacteria-based forensic ecogenomics research by investigating soil fungal community and cadaver decomposition interactions in microcosms with garden soil (20 kg, fresh weight) and domestic pig (sus scrofa domesticus) carc ... | 2015 | 26322496 |
| evidence of long-term gene flow and selection during domestication from analyses of eurasian wild and domestic pig genomes. | traditionally, the process of domestication is assumed to be initiated by humans, involve few individuals and rely on reproductive isolation between wild and domestic forms. we analyzed pig domestication using over 100 genome sequences and tested whether pig domestication followed a traditional linear model or a more complex, reticulate model. we found that the assumptions of traditional models, such as reproductive isolation and strong domestication bottlenecks, are incompatible with the geneti ... | 2015 | 26323058 |
| urinary hepcidin levels in iron-deficient and iron-supplemented piglets correlate with hepcidin hepatic mrna and serum levels and with body iron status. | among livestock, domestic pig (sus scrofa) is a species, in which iron metabolism has been most intensively examined during last decade. the obvious reason for studying the regulation of iron homeostasis especially in young pigs is neonatal iron deficiency anemia commonly occurring in these animals. moreover, supplementation of essentially all commercially reared piglets with iron entails a need for monitoring the efficacy of this routine practice followed in the swine industry for several decad ... | 2015 | 26323096 |
| on-pump nonrobotic total endoscopic coronary artery bypass grafting: an animal study. | total endoscopic coronary artery bypass grafting (tecab) is currently performed exclusively with the help of a telemanipulator. after extensive practice in a biomechanical model, the operation was performed as a nonrobotic approach in an animal study. | 2016 | 26331233 |
| identification and genetic characterization of two porcine astroviruses from domestic piglets in china. | porcine astrovirus are divided into five genotypes. in this study, we identified two porcine astroviruses (astv-ll-1 and astv-ll-2) by using sequence-independent single-primer amplification (sispa) on faecal specimens of healthy domestic piglets younger than 15 days. the detection rate for both was 2.82% (14/497). astv-lls were then sequenced and characterised. phylogenetic analysis revealed that they have the characteristics of porcine astrovirus (pastv) 2 and 5 and have some unique genetic fea ... | 2015 | 26338091 |
| chronically infected wild boar can transmit genotype 3 hepatitis e virus to domestic pigs. | hepatitis e virus (hev) causes acute hepatitis e in humans in developing countries, but sporadic and autochthonous cases do also occur in industrialized nations. in europe, food-borne zoonotic transmission of genotype 3 (gt3) has been associated with the consumption of raw and undercooked products from domestic pig and wild boar. as shown recently, naturally acquired hev gt3 replicates efficiently in experimentally infected wild boar and is transmissible from a wild boar to domestic pigs. genera ... | 2015 | 26344041 |
| hepatitis e: an emerging global disease - from discovery towards control and cure. | hepatitis e is a systemic disease affecting the liver predominantly and caused by infection with the hepatitis e virus (hev). hev has marked genetic heterogeneity and is known to infect several animal species including pigs, boar, deer, mongoose, rabbit, camel, chicken, rats, ferret, bats and cutthroat trout. hev is the sole member of the family hepeviridae and has been divided into 2 genera: orthohepevirus (mammalian and avian hev) and piscihepevirus (trout hev). human hevs included within the ... | 2016 | 26344932 |
| genome-wide analysis of dna methylation in pigs using reduced representation bisulfite sequencing. | dna methylation plays a major role in the epigenetic regulation of gene expression. although a few dna methylation profiling studies of porcine genome which is one of the important biomedical models for human diseases have been reported, the available data are still limited. we tried to study methylation patterns of diverse pig tissues as a study of the international swine methylome consortium to generate the swine reference methylome map to extensively evaluate the methylation profile of the pi ... | 2015 | 26358297 |
| focused post mortem dissection technique for harvest of rete mirabile in domestic swine (sus scrofa). | rete mirabile (rm) of the domestic pig is a popular animal model of arteriovenous malformations. the rm (latin for 'wonderful net)' comprises the arterioarterial portal connecting ascending pharyngeal arteries and the internal carotid arteries, which exists in the skull base of even-toed ungulates. although angiographic access of the rm is relatively easy, its post mortem procurement is complicated and its detailed technique has not been well described. | 2016 | 26359213 |
| experimental infection of ornithodoros erraticus sensu stricto with two portuguese african swine fever virus strains. study of factors involved in the dynamics of infection in ticks. | african swine fever (asf) is a frequently devastating hemorrhagic disease of domestic pigs and wild boar and ornithodoros erraticus sensu stricto argasid ticks are the only biological vectors of african swine fever virus (asfv) known to occur in europe. recently this disease emerged in eastern europe and russian federation, showing a huge potential for a rapid spread between countries. there is some risk of re-emergence of asf in the countries where these ticks exist, that can contribute for the ... | 2015 | 26366570 |
| mitochondrial genome analyses suggest multiple trichuris species in humans, baboons, and pigs from different geographical regions. | the whipworms trichuris trichiura and trichuris suis are two parasitic nematodes of humans and pigs, respectively. although whipworms in human and non-human primates historically have been referred to as t. trichiura, recent reports suggest that several trichuris spp. are found in primates. | 2015 | 26367282 |
| epidemiology of leptospirosis in africa: a systematic review of a neglected zoonosis and a paradigm for 'one health' in africa. | leptospirosis is an important but neglected bacterial zoonosis that has been largely overlooked in africa. in this systematic review, we aimed to summarise and compare current knowledge of: (1) the geographic distribution, prevalence, incidence and diversity of acute human leptospirosis in africa; and (2) the geographic distribution, host range, prevalence and diversity of leptospira spp. infection in animal hosts in africa. | 2015 | 26368568 |
| the fate of human platelets exposed to porcine renal endothelium: a single-pass model of platelet uptake in domestic and genetically modified porcine organs. | thrombocytopenia may represent a significant challenge to the clinical application of solid-organ xenotransplantation. when studied in a pig-to-primate model, consumptive coagulopathy has challenged renal xenografts. new strategies of genetic manipulation have altered porcine carbohydrate profiles to significantly reduce human antibody binding to pig cells. as this process continues to eliminate immunologic barriers to clinical xenotransplantation, the relationship between human platelets and pi ... | 2016 | 26375504 |
| experimental evidence of hepatitis a virus infection in pigs. | hepatitis a virus (hav) is the leading cause of acute viral hepatitis worldwide, with hav infection being restricted to humans and nonhuman primates. in this study, hav infection status was serologically determined in domestic pigs and experimental infections of hav were attempted to verify hav infectivity in pigs. antibodies specific to hav or hav-like agents were detected in 3.5% of serum samples collected from pigs in swine farms. when the pigs were infected intravenously with 2 × 10(5) 50% t ... | 2016 | 26381440 |
| coronary injury score correlates with proliferating cells and alpha-smooth muscle actin expression in stented porcine coronary arteries. | neointimal formation and cell proliferation resulting into in-stent restenosis is a major pathophysiological event following the deployment of stents in the coronary arteries. in this study, we assessed the degree of injury, based on damage to internal elastic lamina, media, external elastic lamina, and adventitia following the intravascular stenting, and its relationship with the degree of smooth muscle cell proliferation. we examined the smooth muscle cell proliferation and their phenotype at ... | 2015 | 26382957 |
| increased mycoplasma hyopneumoniae disease prevalence in domestic hybrids among free-living wild boar. | wildlife immune genes are subject to natural selection exerted by pathogens. in contrast, domestic immune genes are largely protected from pathogen selection by veterinary care. introgression of domestic alleles into the wild could lead to increased disease susceptibility, but observations are scarce due to low introgression rates, low disease prevalence and reduced survival of domestic hybrids. here we report the first observation of a deleterious effect of domestic introgression on disease pre ... | 2015 | 26391376 |
| longitudinal study of the early-life fecal and nasal microbiotas of the domestic pig. | the mammalian microbiota plays a key role in host health and disease susceptibility. however, knowledge of the early-age microbiota of pigs is lacking. the purpose of this study was to use high-throughput next-generation sequencing to characterize the fecal and nasal microbiotas of pigs during early life. | 2015 | 26391877 |
| spatio-temporal analysis of african swine fever in sardinia (2012-2014): trends in domestic pigs and wild boar. | african swine fever (asf) is a notifiable viral disease affecting domestic pigs and wild boars that has been endemic in sardinia since 1978. several risk factors complicate the control of asf in sardinia: generally poor level of biosecurity, traditional breeding practices, illegal behaviour in movements and feeding of pigs, and sporadic occurrence of long-term carriers. a previous study describes the disease in sardinia during 1978-2013. the aim of this study was to gain more in-depth knowledge ... | 2017 | 26392004 |
| novel real-time pcr detection assay for brucella suis. | brucella suis is the causative agent of brucellosis in suidae and is differentiated into five biovars (bv). biovars 1 and 3 possess zoonotic potential and can infect humans, whereas biovar 2 represents the main source of brucellosis in feral and domestic pigs in europe. both aspects, the zoonotic threat and the economic loss, emphasize the necessity to monitor feral and domestic pig populations. available serological or pcr based methods lack sensitivity and specificity. | 2015 | 26392898 |
| signaling pathways related to protein synthesis and amino acid concentration in pig skeletal muscles depend on the dietary protein level, genotype and developmental stages. | muscle growth is regulated by the homeostatic balance of the biosynthesis and degradation of muscle proteins. to elucidate the molecular interactions among diet, pig genotype, and physiological stage, we examined the effect of dietary protein concentration, pig genotype, and physiological stages on amino acid (aa) pools, protein deposition, and related signaling pathways in different types of skeletal muscles. the study used 48 landrace pigs and 48 pure-bred bama mini-pigs assigned to each of 2 ... | 2015 | 26394157 |
| histochemical study of heparin-positive mast cells in the terminal part of porcine ductus choledochus and papilla duodeni major. | the study presented in detail the localization and density of mast cells (mcs) in the intramural part of the common bile duct (cbd) and in the major duodenal papilla (mdp) of domestic swine. mcs' density (number/mm(2) ) in different layers of both of the duct and papilla was evaluated after toluidine blue staining. their number was higher in the lamina propria mucosae than in the tunica muscularis of the studied structures. the localization of berberine-positive, (heparin containing) mcs and the ... | 2016 | 26394797 |
| authentication of beef, carabeef, chevon, mutton and pork by a pcr-rflp assay of mitochondrial cytb gene. | authentication of meat assumes significance in view of religious, quality assurance, food safety, public health, conservation and legal concerns. here, we describe a pcr-rflp (polymerase chain reaction- restriction fragment length polymorphism) assay targeting mitochondrial cytochrome-b gene for the identification of meats of five most common food animals namely cattle, buffalo, goat, sheep and pig. a pair of forward and reverse primers (vph-f & vph-r) amplifying a conserved region (168-776 bp) ... | 2012 | 26396346 |
| blood collection from the orbital sinus of swine. | swine (sus scrofa domesticus) are increasingly used as animal models in biomedical research. often blood samples from these animals are needed for research protocols, but phlebotomy can be difficult, especially with young swine. this column describes a relatively simple technique for blood collection that can be used specifically with young swine. | 2015 | 26398611 |
| alaria alata mesocercariae among feral cats and badgers, denmark. | 2015 | 26402302 | |
| development of real-time pcr assay for detection of porcine circovirus-like virus p1 in domestic pigs in china. | the porcine circovirus-like agent p1 is a newly discovered dna virus with a single-stranded circular genome that is highly homologous to that of porcine circovirus type 2. p1 infection can cause symptoms resembling postweaning multisystemic wasting syndrome. this study aims to develop a rapid, sensitive and specific method to detect p1. | 2015 | 26404908 |
| assessment of ttx-s and ttx-r action potential conduction along neurites of ngf and gdnf cultured porcine drg somata. | nine isoforms of voltage-gated sodium channels (nav) have been characterized and in excitable tissues they are responsible for the initiation and conduction of action potentials. for primary afferent neurons residing in dorsal root ganglia (drg), individual neurons may express multiple nav isoforms extending the neuron's functional capabilities. since expression of nav isoforms can be differentially regulated by neurotrophic factors we have examined the functional consequences of exposure to eit ... | 2015 | 26407014 |
| distinction of non-ischemia inducing versus ischemia inducing coronary stenosis by fluorescent cardiac imaging. | intraoperative fluorescent cardiac imaging (fci) can quantitatively assess myocardial perfusion abnormalities produced by graded flow-limiting coronary stenosis (fls), but there are no data to distinguish fls from non flow-limiting stenosis (nfls) to determine their functional significance. the aim of the study was to evaluate whether non-ischemia inducing nfls can be quantified and differentiated from fls by fci technology. data are compared to gold standard fluorescent microspheres (fm). 11 do ... | 2016 | 26408104 |
| human recombinant activated protein c-coated stent for the prevention of restenosis in porcine coronary arteries. | activated protein c (apc), an endogenous protein, inhibits inflammation and thrombosis and interrupts the coagulation cascade. here, we investigated the effect of human recombinant apc on the development of neointimal hyperplasia in porcine coronary arteries. yukon choice bare metal stents were coated with 2.6 µg apc/mm(2). under general anesthesia, apc-coated and bare stents were implanted in the left anterior descending and circumflex coronary arteries of 10 domestic pigs. during the 4-week fo ... | 2015 | 26411437 |
| spatiotemporal trends in the discovery of new swine infectious agents. | a literature review was conducted to assess the spatiotemporal trend and diversity of infectious agents that were newly found in pigs between 1985 and 2010. we identified 173 new variants from 91 species, of which 73 species had not been previously described in pigs. these new species, of which one third was zoonotic, were taxonomically diverse. they were identified throughout the study period, predominantly in the main pork producing countries, with the rate of discovery of new virus variants d ... | 2015 | 26412219 |