Publications

bipolar multi-electrode balloon catheter radiofrequency renal denervation with the vessix system: preclinical safety evaluation.	a bipolar multi-electrode 7 fr-compatible balloon-catheter radiofrequency (rf) renal denervation system (vessix™ renal denervation system; boston scientific, marlborough, ma, usa) was evaluated for safety in domestic swine.	2015	25701511
changes in lens stiffness due to capsular opacification in accommodative lens refilling.	accommodation may be restored to presbyopic lenses by refilling the lens capsular bag with a soft polymer. after this accommodative lens refilling prevention of capsular opacification is a requirement, since capsular opacification leads to a decreased clarity of the refilled lens. it has been hypothesized that capsular fibrosis causing the capsular opacification results in increased stiffness of the lens capsular bag, therewith contributing to a decrease in accommodative amplitude of the lens. h ...	2015	25704214
evaluation of the plasmatic and parenchymal elution kinetics of two different irinotecan-loaded drug-eluting embolics in a pig model.	to evaluate and compare irinotecan elution kinetics of two drug-eluting embolic agents in a porcine model.	2015	25704223
reproductive ratio for the local spread of african swine fever in wild boars in the russian federation.	african swine fever (asf) has caused the swine industry of the russian federation substantial economic losses over the last 7 years, and the disease spread from there to a number of neighbouring countries. wild boar has been involved in the spread of the disease both at local and at transboundary levels. understanding asf dynamics in wild boars is prerequisite to preventing the spread and to designing and applying effective surveillance and control plans. the reproductive ratio (r0) is an epidem ...	2016	25704269
tissue concentrations of sulfamethazine and tetracycline hydrochloride of swine (sus scrofa domestica) as it relates to withdrawal methods for international export.	the use of water medications is a common practice in the us swine industry to treat and prevent infections in swine herds with minimal labor and without risk of needle breakage. there are concerns that fda-approved withdrawal times (wdt) may be inadequate for several water medications when exporting pork products to countries where mrls (maximum residue limits) are lower than us tolerance levels. in this study, withdrawal intervals (wdi) were estimated for pigs when dosed with tetracycline and s ...	2015	25707857
[genetic components and the uncertainty of the phenotypic realization of the mass of newborns in domestic pigs sus scrofa l].	in this article, we discuss the features of the genetic determination of a continuous quantitative trait, the mass of newborn offspring in populations of the domestic pig. we defined several components that determine the phenotypic trait, such as the maternal effect, complete dominance, interaction of the parental alleles in the genotype of the offspring, and the uncertainty of phenotypic realization of genotype. we found that a phenotypic trait of high genetic determinacy can also have a maximu ...	2014	25711014
[classifier of domestic pig (sus scrofa domesticus) automatic chromosome identification].	automated chromosome classification is an essential task in cytogenetics of animals and plants. until now the automatic karyotyping systems were obtained only for human chromosomes. the main aim of this study was to develop the automatic pig chromosome classifier using image processing software "videotest-karyo 3.1". to solve this problem 1578 chromosomes from 47 metaphases were used. the constructed classifier was checked with the use of additional sample of metaphases classified in fully autom ...	2014	25711088
on genetic differentiation between domestic pigs and tibetan wild boars.		2015	25711861
reply to 'on genetic differentiation between domestic pigs and tibetan wild boars'.		2015	25711862
secondary structure analysis of swine pasivirus (family picornaviridae) rna reveals a type-iv ires and a parechovirus-like 3' utr organization.	the potential rna structures of the 5' and 3' untranslated regions (utrs) and cis-acting replication elements (cres) of a novel pasivirus (pav) genotype (family picornaviridae) were analysed. pav-a3 (km259923) was identified in a faecal sample from a domestic pig in hungary with posterior paraplegia of unknown etiology. based on likely structural features of the 5' utr, the pasiviruses were inferred to possess hepacivirus/pestivirus-like type-iv ires. the pasivirus cre was mapped to the 2b genom ...	2015	25716922
the relation between catheter occlusion and backflow during intraparenchymal cerebral infusions.	background/aims: the distribution of infusate into the brain by convection-enhanced delivery can be affected by backflow along the catheter shaft. this work assesses the following: (1) whether tissue coring and occlusion of the catheter lumen occurs when an open end-port catheter is inserted, (2) whether there is a relationship between intracatheter pressure and backflow, and (3) whether catheter occlusion increases backflow. methods: freshly excised monkey brains were used to assess tissue cori ...	2015	25721097
west nile virus serosurveillance in pigs, wild boars, and roe deer in serbia.	west nile virus (wnv) is maintained in nature in an enzootic transmission cycle between birds and mosquitoes, but it also infects many other vertebrates, including humans and horses, in which it can induce severe neurological diseases; however, data about virus circulation in other mammals is scarce. wnv has a history of recent outbreaks in europe, including serbia, where it was identified for the first time in 2010 in mosquitoes and in 2012 in birds and humans, being responsible for over 300 co ...	2015	25724332
evidence for a link between tail biting and central monoamine metabolism in pigs (sus scrofa domestica).	tail biting in pigs is a major welfare problem within the swine industry. even though there is plenty of information on housing and management-related risk factors, the biological bases of this behavioral problem are poorly understood. the aim of this study was to investigate a possible link between tail biting, based on behavioral recordings of pigs during an ongoing outbreak, and certain neurotransmitters in different brain regions of these pigs. we used a total of 33 pigs at a farm with a lon ...	2015	25728243
effectiveness of microsatellite and single nucleotide polymorphism markers for parentage analysis in european domestic pigs.	parentage analysis and individual identification are recent, promising methods that have been applied to evolutionary and ecological studies, as well as conservation management. parental exclusion relying on polymorphic microsatellites has been used worldwide in parentage determination, while the low mutation rate and genotyping error rate of single nucleotide polymorphisms (snps) make them another important marker for pedigree tracing. here, we compared the effectiveness of microsatellites and ...	2015	25730075
[assessment of bovine pericardium (tutomesh®) and coated polypropylene (proceed®) in the para-oesophageal position in a pig model].	hiatal hernias are nowadays increasingly treated with meshes. often, biological implants are being used for this application. oesophageal perforations have been reported as rare but serious complications from the application of synthetic meshes at the oesophageal hiatus. the role of the different mesh types has not been clearly established by experimental research so far. in the present large animal model, we investigated two implant types (tutomesh® and proceed®) with respect to their biocompat ...	2015	25738433
a novel porcine model for chemically inducible crohn's-like reaction.	new animal models provide insights into the pathogenesis of different types of inflammatory bowel disease as well as novel pathways for new therapeutic options. however, the scarcity of large animal models hinders the research and development of new surgical procedures and technological devices in inflammatory bowel disease surgery. common small animal inducible models involve chemical agents that result in the development of acute intestinal inflammation.	2015	25739171
experimental infection with the toxoplasma gondii me-49 strain in the brazilian br-1 mini pig is a suitable animal model for human toxoplasmosis.	toxoplasma gondii causes toxoplasmosis, a worldwide disease. experimentation with pigs is necessary for the development of new therapeutic approaches to human diseases. br-1 mini pigs were intramuscularly infected with t. gondii with tachyzoites (rh strain) or orally infected with cysts (me-49 strain). haematology and serum biochemistry were analysed and buffy coat cells were inoculated in mice to determine tachyzoite circulation. no alterations were observed in erythrocyte and platelet values; ...	2015	25742268
prevalence of emerging porcine parvoviruses and their co-infections with porcine circovirus type 2 in china.	a total of 450 samples from domestic pigs in china were tested for porcine parvoviruses (ppvs) and co-infections with porcine circovirus type 2 (pcv2), and their complete capsid genes were sequenced. the prevalence of ppv1, ppv2, ppv3, ppv4, and pcv2 was 5.56 %, 39.56 %, 45.11 %, 21.56 %, and 47.33 %, respectively, and co-infection with pcv2 occurred in 4 % (ppv1), 22.44 % (ppv2), 24 % (ppv3), and 12 % (ppv4) of the samples. phylogenetic analysis revealed two main lineages for each virus, and re ...	2015	25742931
crimped braided sleeves for soft, actuating arm in robotic abdominal surgery.	this paper investigates different types of crimped, braided sleeve used for a soft arm for robotic abdominal surgery, with the sleeve required to contain balloon expansion in the pneumatically actuating arm while it follows the required bending, elongation and diameter reduction of the arm.	2015	25744663
investigating the role of free-ranging wild boar (sus scrofa) in the re-emergence of enzootic pneumonia in domestic pig herds: a pathological, prevalence and risk-factor study.	enzootic pneumonia (ep) caused by mycoplasma hyopneumoniae has a significant economic impact on domestic pig production. a control program carried out from 1999 to 2003 successfully reduced disease occurrence in domestic pigs in switzerland, but recurrent outbreaks suggested a potential role of free-ranging wild boar (sus scrofa) as a source of re-infection. since little is known on the epidemiology of ep in wild boar populations, our aims were: (1) to estimate the prevalence of m. hyopneumoniae ...	2015	25747151
establishing a reproducible hypertrophic scar following thermal injury: a porcine model.	our complete understanding of hypertrophic scarring is still deficient, as portrayed by the poor clinical outcomes when treating them. to address the need for alternative treatment strategies, we assess the swine animal burn model as an initial approach for immature scar evaluation and therapeutic application.	2015	25750848
enhanced perfusion during advanced life support improves survival with favorable neurologic function in a porcine model of refractory cardiac arrest.	to improve the likelihood for survival with favorable neurologic function after cardiac arrest, we assessed a new advanced life support approach using active compression-decompression cardiopulmonary resuscitation plus an intrathoracic pressure regulator.	2015	25756411
a genome-wide scan for signatures of directional selection in domesticated pigs.	animal domestication involved drastic phenotypic changes driven by strong artificial selection and also resulted in new populations of breeds, established by humans. this study aims to identify genes that show evidence of recent artificial selection during pig domestication.	2015	25765548
prevalence of hepatitis e virus in populations of wild animals in comparison with animals bred in game enclosures.	hepatitis e virus (hev) is now accepted as a zoonotic virus, and domestic pigs, wild boars and deer are recognised as natural reservoirs of the pathogen. in this study, 762 animals (wild boars, fallow deer, red deer, sika deer, roe deer and mouflons) originating from the wild and from game enclosures were tested for the presence of hev rna by qrt-pcr. hev rna was detected in wild boars (96/450), red deer (2/169), roe deer (1/30) and mouflons (5/39). the sequence relationship between hev isolates ...	2015	25771162
atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins.	atrial fibrillation (af) and heart failure (hf) are two of the most common cardiovascular diseases. they often coexist and account for significant morbidity and mortality. alterations in cellular ca2+ homeostasis play a critical role in af initiation and maintenance. this study was designed to specifically elucidate af-associated remodeling of atrial ca2+ cycling in the presence of mild hf. af was induced in domestic pigs by atrial burst pacing. the animals underwent electrophysiologic and echoc ...	2015	25775120
serological and molecular evidence of hepadnavirus infection in swine.	recently, investigations in a swine herd identified evidence of the existence of a novel member of the hepadnavirus family endemic in swine. the aim of this study was to investigate the serological and molecular markers of hepadnavirus circulation in brazilian domestic swine and wild boar herds, and to evaluate the identity with hbv and other hepadnaviruses reported previously.	2015	25780820
the investigation of interspecies diversity of erythrocyte aggregation properties by two different photometric methods in four animal species.	among the haemorheological parameters, red blood cell (rbc) aggregation shows the largest interspecies diversity, and often controversial data can be found in the literature, besides the methodology-dependent issues. in this present investigation, we compared four experimental/laboratory animal species' rbc aggregation by two different photometric methods for better revealing the differences. blood samples (k3-edta, 1.5 mg/ml) were taken from female animals: 16 inbred mice (mus musculus, cardiac ...	2015	25788141
selection and validation of reference genes for qrt-pcr expression analysis of candidate genes involved in olfactory communication in the butterfly bicyclus anynana.	real-time quantitative reverse transcription pcr (qrt-pcr) is a technique widely used to quantify the transcriptional expression level of candidate genes. qrt-pcr requires the selection of one or several suitable reference genes, whose expression profiles remain stable across conditions, to normalize the qrt-pcr expression profiles of candidate genes. although several butterfly species (lepidoptera) have become important models in molecular evolutionary ecology, so far no study aimed at identify ...	2015	25793735
standardization of the experimental autoimmune myasthenia gravis (eamg) model by immunization of rats with torpedo californica acetylcholine receptors--recommendations for methods and experimental designs.	myasthenia gravis (mg) with antibodies against the acetylcholine receptor (achr) is characterized by a chronic, fatigable weakness of voluntary muscles. the production of autoantibodies involves the dysregulation of t cells which provide the environment for the development of autoreactive b cells. the symptoms are caused by destruction of the postsynaptic membrane and degradation of the achr by igg autoantibodies, predominantly of the g1 and g3 subclasses. active immunization of animals with ach ...	2015	25796590
a study of lymphoid organs and serum proinflammatory cytokines in pigs infected with african swine fever virus genotype ii.	african swine fever virus (asfv), the causative agent of one of the most important viral diseases of domestic pigs for which no vaccine is available, causes immune system disorders in infected animals. in this study, the serum levels of proinflammatory cytokines, as well as the histological and cellular constitution of lymphoid organs of pigs infected with asfv genotype ii were investigated. the results showed a high degree of lymphocyte depletion in the lymphoid organs, particularly in the sple ...	2015	25797197
humans (homo sapiens) judge the emotional content of piglet (sus scrofa domestica) calls based on simple acoustic parameters, not personality, empathy, nor attitude toward animals.	the vocal expression of emotion is likely driven by shared physiological principles among species. however, which acoustic features promote decoding of emotional state and how the decoding is affected by their listener's psychology remain poorly understood. here we tested how acoustic features of piglet vocalizations interact with psychological profiles of human listeners to affect judgments of emotional content of heterospecific vocalizations. we played back 48 piglet call sequences recorded in ...	2015	25798794
snp marker discovery in koala tlr genes.	toll-like receptors (tlrs) play a crucial role in the early defence against invading pathogens, yet our understanding of tlrs in marsupial immunity is limited. here, we describe the characterisation of nine tlrs from a koala immune tissue transcriptome and one tlr from a draft sequence of the koala genome and the subsequent development of an assay to study genetic diversity in these genes. we surveyed genetic diversity in 20 koalas from new south wales, australia and showed that one gene, tlr10 ...	2015	25799012
functional characterization of a novel lytic phage ecsw isolated from sus scrofa domesticus and its potential for phage therapy.	in this study, multi-drug resistant escherichia coli sw1 (e. coli sw1) and active lytic phage ecsw was isolated from feces samples of sus scrofa domesticus (piglet) suffering from diarrhea. transmission electron microscopy (tem) indicated that isolated ecsw belongs to the myoviridae family with an icosahedral head (80 ± 4) and a long tail (180 ± 5 nm). the ecsw phage genome size was estimated to be approximately 75 kb of double-stranded dna (dsdna). phage dynamic studies show that the latent per ...	2015	25805216
effects of bovine lactoferrin in surgically created bone defects on bone regeneration around implants.	the aim of this experimental study was to evaluate the effect of bovine lactoferrin (blf)-loaded gelatin microspheres (gm) used in combination with anorganic bovine bone on bone regeneration in surgically created bone defects around tooth implants. twenty-four uniform bone defects were created in the frontal bone via an extraoral approach in 12 domestic pigs. twenty-four implants were placed at the center of the defects. in eight animals one of these defects was filled with 0.3 ml anorganic bovi ...	2015	25807903
experimental infection of domestic pigs with african swine fever virus lithuania 2014 genotype ii field isolate.	an experimental infection was conducted to evaluate horizontal transmission, clinical, virological and humoral response induced in domestic pigs infected with african swine fever (asf) genotype ii virus circulating in 2014 into the european union (eu). ten naive pigs were placed in contact with eight pigs experimentally inoculated with the lithuanian lt14/1490 asf virus (asfv) responsible for the first asf case detected in wild boar in lithuania in january 2014. clinical examination and rectal t ...	2017	25808027
wild boars from sweden, austria, the czech republic and japan possess intact mannose-binding lectin 2 (mbl2) genes.	the two-nucleotide deletion recently detected in the mannose-binding lectin 2 gene in purebred and crossbred domestic pigs was not found among 68 wild boars representing 4 populations from europe and asia. this suggests that the deletion is a result of breeding and/or genetic drift/bottle necks.	2015	25809846
african swine fever virus georgia isolate harboring deletions of mgf360 and mgf505 genes is attenuated in swine and confers protection against challenge with virulent parental virus.	african swine fever virus (asfv) is the etiological agent of a contagious and often lethal disease of domestic pigs that has significant economic consequences for the swine industry. the control of african swine fever (asf) has been hampered by the unavailability of vaccines. experimental vaccines have been developed using genetically modified live attenuated asfvs where viral genes involved in virus virulence were removed from the genome. multigene family 360 (mgf360) and mgf505 represent a gro ...	2015	25810553
flexible growing rods: a pilot study to determine if polymer rod constructs may provide stability to skeletally immature spines.	surgical treatments for early onset scoliosis (eos), including growing rod constructs, involve many complications. some are due to biomechanical factors. a construct that is more flexible than current instrumentation systems may reduce complications. the purpose of this preliminary study was to determine spine range of motion (rom) after implantation of simulated growing rod constructs with a range of clinically relevant structural properties. the hypothesis was that rom of spines instrumented w ...	2015	25810752
distribution and molecular characterization of hepatitis e virus in domestic animals and wildlife in croatia.	hepatitis e is becoming a growing health concern in european countries as an increase of sporadic human cases of unknown origin has been recorded lately. its causative agent, hepatitis e virus (hev), is known to have zoonotic potential and thus the role of domestic and wild animals in the chain of viral spread should be considered when investigating risk factors and the epidemiology of the disease. a comprehensive survey based on viral rna detection was carried out in croatia including blood, sp ...	2015	25820413
the chimerical genome of isla del coco feral pigs (costa rica), an isolated population since 1793 but with remarkable levels of diversity.	the history of domestic species and of their wild ancestors is not a simple one, and feral processes can clarify key aspects of this history, including the adaptive processes triggered by new environments. here, we provide a comprehensive genomic study of isla del coco (costa rica) feral pigs, a unique population that was allegedly founded by two individuals and has remained isolated since 1793. using snp arrays and genome sequencing, we show that cocos pigs are hybrids between asian and europea ...	2015	25827466
the effects of altered maxillary growth on patterns of mandibular rotation in a pig model.	a thorough understanding of influence of maxillary growth on patterns of mandibular rotation during development is important with regard to the treatment of skeletal discrepancies. in the present study, we examined whether experimentally altered maxillary position has a significant influence on patterns of mandibular rotation in a pig model.	2015	25841069
trichinellosis in vietnam.	trichinellosis is a zoonotic parasitic disease with a worldwide distribution. the aim of this work was to describe the epidemiological and clinical data of five outbreaks of trichinellosis, which affected ethnic minorities living in remote mountainous areas of northwestern vietnam from 1970 to 2012. trichinellosis was diagnosed in 126 patients, of which 11 (8.7%) were hospitalized and 8 (6.3%) died. all infected people had consumed raw pork from backyard and roaming pigs or wild boar at wedding, ...	2015	25846295
staphylococcus aureus induces hypoxia and cellular damage in porcine dermal explants.	we developed a porcine dermal explant model to determine the extent to which staphylococcus aureus biofilm communities deplete oxygen, change ph, and produce damage in underlying tissue. microelectrode measurements demonstrated that dissolved oxygen (do) in biofilm-free dermal tissue was 4.45 ± 1.17 mg/liter, while do levels for biofilm-infected tissue declined sharply from the surface, with no measurable oxygen detectable in the underlying dermal tissue. magnetic resonance imaging demonstrated ...	2015	25847960
corrigendum: cellular scaling rules for the brain of artiodactyla include a highly folded cortex with few neurons.	[this corrects the article on p. 128 in vol. 8, pmid: 25429261.].	2015	25859187
neurophysiological monitoring during acute and progressive experimentally induced compression injury of the spinal cord in pigs.	to evaluate the degree of acute or progressive lateral compression needed to cause neurologic injury to the spinal cord assessed by electrophysiological monitoring.	2017	25862652
analysis of carcass and meat quality traits and nutritional values of hybrid wild boars under different crossing systems.	the aim of this study was to analyze the results of two crossing systems between wild boars and different domesticated pig breeds. hybrid wild boars were produced by crossing captured wild boars with meishan pigs and ly sows according to the traditional production system. the resultant commercial hybrids were black and white in coat color, respectively. significant differences were found in the carcass and meat quality traits and nutritional values between these two hybrid wild boars. compared w ...	2015	25867408
effect of body mass and clothing on carrion entomofauna.	carcass mass largely affects pattern and rate of carrion decomposition. supposedly, it is similarly important for carrion entomofauna; however, most of its likely effects have not been tested experimentally. here, simultaneous effects of carcass mass and clothing are analyzed. a factorial block experiment with four levels of carcass mass (small carcasses 5-15 kg, medium carcasses 15.1-30 kg, medium/large carcasses 35-50 kg, large carcasses 55-70 kg) and two levels of carcass clothing (clothed an ...	2015	25874664
the first detection of prrsv in wild boars in slovakia.	prrsv was confirmed by a nested rt-pcr in two out of 129 samples originating from wild boars from eastern slovakia. virus isolates were genetically typed in orf7 as prrsv-1, eu-1 with 99.7% (sample 14wb) and 100% (sample 10wb) nucleotide sequence similarity to lelystad reference strain and porcilis prrs vaccine strain, respectively. the origin of prrsv in these wild boars is unclear but it is highly likely that virus was transmitted from vaccinated domestic pigs to wild boars.	2015	25876282
development of a new lamp assay for the detection of csfv strains from cuba: a proof-of-concept study.	classical swine fever (csf) is a devastating animal disease of great economic impact worldwide. in many countries, csf has been endemic for decades, and vaccination of domestic pigs is one of the measures to control the disease. consequently, differentiating infected from vaccinated animals by antibody elisa screening is not applicable. in some countries, such as cuba, lack of molecular techniques for sensitive, rapid and reliable detection of virus genomes is a critical point. to overcome this ...	2015	25877822
spatial characterization of colonies of the flying fox bat, a carrier of nipah virus in thailand.	a major reservoir of nipah virus is believed to be the flying fox genus pteropus, a fruit bat distributed across many of the world's tropical and sub-tropical areas. the emergence of the virus and its zoonotic transmission to livestock and humans have been linked to losses in the bat's habitat. nipah has been identified in a number of indigenous flying fox populations in thailand. while no evidence of infection in domestic pigs or people has been found to date, pig farming is an active agricultu ...	2015	25880385
prevalence of infection with toxoplasma gondii in landrace and mixed breed pigs slaughtered in baja california sur state, mexico.	we performed a cross-sectional study to determine the seroprevalence of toxoplasma gondii infection in 308 domestic pigs slaughtered in la paz, baja california sur state, mexico using the modified agglutination test (mat, cut off 1:25). forty (13%) of the 308 pigs were seropositive with mat titers of 1:25 in 16, 1:50 in 5, 1:100 in 4, 1:200 in 5, 1:400 in 3, 1:800 in 3, 1:1600 in 2, and 1:3200 in 2. multivariate analysis of pigs' characteristics showed that seropositivity to t. gondii was negati ...	2015	25883799
exploring the genetic signature of body size in yucatan miniature pig.	since being domesticated about 10,000-12,000 years ago, domestic pigs (sus scrofa domesticus) have been selected for traits of economic importance, in particular large body size. however, yucatan miniature pigs have been selected for small body size to withstand high temperature environment and for laboratory use. this renders the yucatan miniature pig a valuable model for understanding the evolution of body size. we investigate the genetic signature for selection of body size in the yucatan min ...	2015	25885114
genome-wide association analyses reveal significant loci and strong candidate genes for growth and fatness traits in two pig populations.	recently, genome-wide association studies (gwas) have been reported on various pig traits. we performed a gwas to analyze 22 traits related to growth and fatness on two pig populations: a white duroc × erhualian f2 intercross population and a chinese sutai half-sib population.	2015	25885760
serological prevalence of viral agents that induce reproductive failure in south korean wild boar.	viral agents associated with reproductive failure such as aujeszky's disease virus (adv), encephalomyocarditis virus (emcv), and porcine parvovirus (ppv) have also been identified in european wild boar. to screen for the presence of antibodies against adv, emcv, and ppv from wild boar (sus scrofa) in south korea, 481 serum samples were collected from wild boar hunted between december 2010 and may 2011.	2015	25888836
pluripotent and metabolic features of two types of porcine ipscs derived from defined mouse and human es cell culture conditions.	the domestic pig is an excellent animal model for stem cell research and clinical medicine. there is still no suitable culture condition to generate authentic porcine embryonic stem cells (pescs) and high quality porcine induced pluripotent stem cells (pipscs). in this study, we found that culture conditions affected pluripotent and metabolic features of pipscs. using defined human embryonic stem cell (hesc) and mouse esc (mesc) culture conditions, we generated two types of pipscs, one of which ...	2015	25893435
tvp1022: a novel cardioprotective drug attenuates left ventricular remodeling after ischemia/reperfusion in pigs.	the current cornerstone treatment of myocardial infarction (mi) is restoration of coronary blood flow by means of thrombolytic therapy or primary percutaneous coronary intervention. however, reperfusion of ischemic myocardium can actually provoke tissue damage, defined as "ischemia-reperfusion (i/r) injury." tvp1022 [the s-isomer of rasagiline (azilect), fda-approved anti-parkinson's drug] was found to exert cardioprotective activities against various cardiac insults, such as chronic heart failu ...	2015	25900266
autophagy portends the level of cardiac hypertrophy in experimental hypertensive swine model.	left ventricular (lv) hypertrophy (lvh) plays an important role in hypertensive heart disease, and may be accompanied by myocardial autophagy. however, the pattern of autophagy during evolution of lvh is unclear. we hypothesized that autophagy activation indicates advancing cardiac lvh with tissue remodeling.	2016	25904651
real-time in-vivo imaging of pulmonary capillary perfusion using probe-based confocal laser scanning endomicroscopy in pigs: an interventional laboratory study.	little is known about real-time in-vivo microscopy of pulmonary capillary perfusion because current microscopy requires direct access to lung tissue with surgical intervention such as the thoracic-window technique and open-lung model.	2015	25906262
detection and molecular analysis of pseudorabies virus strains isolated from dogs and a wild boar in italy.	aujeszky's disease (ad) is one of the most economically important diseases of farmed pigs. wild boars can act as reservoirs and might represent a potential threat for domestic animals, including dogs. the aim of this study was to report the results of an ad survey based on the pseudorabies virus (prv) genome detection in samples of dogs clinically suspected of ad and of wild boars collected during four consecutive hunting seasons in the period 2010-2014. genomic characterization was based on the ...	2015	25912160
fluid dynamics simulation of right ventricular outflow tract oversizing.	repair of the right ventricular outflow tract (rvot) in paediatric cardiac surgery remains challenging due to the high reoperation rate. intimal hyperplasia and consequent arteriosclerosis is one of the most important limitation factors for graft durability. since local shear stress and pressure are predictive elements for intimal hyperplasia and wall degeneration, we sought to determine in an oversized 12-mm rvot model, with computed fluid dynamics simulation, the local haemodynamical factors t ...	2015	25912476
trichinella pseudospiralis in the iberian peninsula.	nematode worms of the genus trichinella are zoonotic parasites circulating in most continents, including europe. in spain, trichinella spiralis and trichinella britovi are highly prevalent in wildlife but seldom in domestic pigs. in portugal, only t. britovi was documented in wild carnivores. in the period 2006-2013 in spain, 384 (0.0001%) pigs and 1399 (0.20%) wild boars (sus scrofa) were positive for trichinella spp. larvae, which were identified as t. spiralis or t. britovi. in 2014, trichine ...	2015	25913596
course and transmission characteristics of oral low-dose infection of domestic pigs and european wild boar with a caucasian african swine fever virus isolate.	in 2007, african swine fever virus (asfv) was introduced into the transcaucasian countries and russia. since then, it has spread alarmingly and reached the european union. asfv strains are highly virulent and lead to almost 100% mortality under experimental conditions. however, the possibility of dose-dependent disease courses has been discussed. for this reason, a study was undertaken to assess the risk of chronic disease and the establishment of carriers upon low-dose oronasal infection of dom ...	2015	25916610
a next generation semiconductor based sequencing approach for the identification of meat species in dna mixtures.	the identification of the species of origin of meat and meat products is an important issue to prevent and detect frauds that might have economic, ethical and health implications. in this paper we evaluated the potential of the next generation semiconductor based sequencing technology (ion torrent personal genome machine) for the identification of dna from meat species (pig, horse, cattle, sheep, rabbit, chicken, turkey, pheasant, duck, goose and pigeon) as well as from human and rat in dna mixt ...	2015	25923709
expression of vpac1 receptor at the level of mrna and protein in the porcine female reproductive system.	the presence and distribution of vasoactive intestinal polypeptide (vip) receptor vpac1 was studied in the ovary, oviduct and uterus (uterine horn and cervix) of the domestic pig using methods of molecular biology (rt-pcr and immunoblot) and immunohistochemistry. the expression of vpac1 receptor at mrna level was confirmed with rt-pcr in all the studied parts of the porcine female reproductive system by the presence of 525 bp pcr product and at the level of proteins by the detection of 46 kda pr ...	2015	25928928
estimating the basic reproductive number (r0) for african swine fever virus (asfv) transmission between pig herds in uganda.	african swine fever (asf) is a highly contagious, lethal and economically devastating haemorrhagic disease of domestic pigs. insights into the dynamics and scale of virus transmission can be obtained from estimates of the basic reproduction number (r0). we estimate r0 for asf virus in small holder, free-range pig production system in gulu, uganda. the estimation was based on data collected from outbreaks that affected 43 villages (out of the 289 villages with an overall pig population of 26,570) ...	2015	25938429
the assessment of african swine fever virus risk to belgium early 2014, using the quick and semiquantitative pandora screening protocol.	a risk assessment was organized during the early eu asf outbreaks of early 2014 (february-april) and performed in cooperation with 15 belgian and european experts on asfv and its epidemiology in pigs/wild boar. african swine fever (asf) is considered as one of the most dangerous infectious pig diseases, causing many outbreaks. since the end of 2013 - early 2014, several outbreaks within the european union (lithuania, poland, estonia and latvia) were reported to oie, which prompted several risk a ...	2017	25939453
a nuclear magnetic resonance spectroscopy as a method for evaluation of in vivo poly-l-lactide biodegradation kinetics from stent-polymer matrices: an experimental study utilizing porcine model of in-stent restenosis.	we aimed to comprehensively evaluate poly-lactide polymer degradation and sirolimus release kinetics from a drug-eluting stent matrix in the in vivo setting using a nuclear magnetic resonance (nmr) method.	2016	25944843
malignant catarrhal fever in a vietnamese pot-bellied pig. a potential threat to pigs in mixed-species exhibits?	malignant catarrhal fever (mcf) represents a sporadic and often fatal disease in various ungulate species including rarely swine. a close contact between susceptible and reservoir species of ovine herpesvirus-2 (ovhv-2) is a requirement for virus transmission. as in ruminants, a rapid course of disease with lymphohistiocytic meningoencephalitis and necrotizing vasculitis in multiple organs is frequently seen in porcine mcf. this report describes a case of mcf in a vietnamese pot-bellied pig, whi ...	2015	25947878
pirnas from pig testis provide evidence for a conserved role of the piwi pathway in post-transcriptional gene regulation in mammals.	piwi-interacting (pi-) rnas guide germline-expressed piwi proteins in order to suppress the activity of transposable elements (tes). but notably, the majority of pachytene pirnas in mammalian testes is not related to tes. this raises the question of whether the piwi/pirna pathway exerts functions beyond te silencing. although gene-derived pirnas were described many times, a possible gene-regulatory function was doubted due to the absence of antisense pirnas. here we sequenced and analyzed pirnas ...	2015	25950437
yersinia enterocolitica isolates from wild boars hunted in lower saxony, germany.	yersiniosis is strongly associated with the consumption of pork contaminated with enteropathogenic yersinia enterocolitica, which is harbored by domestic pigs without showing clinical signs of disease. in contrast to data on y. enterocolitica isolated from conventionally reared swine, investigations into the occurrence of y. enterocolitica in wild boars in germany are rare. the objectives of the study were to get knowledge about these bacteria and their occurrence in wild boars hunted in norther ...	2015	25956779
a novel swine model of spontaneous hypertension with sympathetic hyperactivity responds well to renal denervation.	the large animal model of arterial hypertension is very valuable to test the antihypertensive drugs and devices. we characterized a novel swine model of spontaneous hypertension and investigated its response to renal denervation (rdn).	2016	25958303
transfection of rna from organ samples of infected animals represents a highly sensitive method for virus detection and recovery of classical swine fever virus.	translation and replication of positive stranded rna viruses are directly initiated in the cellular cytoplasm after uncoating of the viral genome. accordingly, infectious virus can be generated by transfection of rna genomes into susceptible cells. in the present study, efficiency of conventional virus isolation after inoculation of cells with infectious sample material was compared to virus recovery after transfection of total rna derived from organ samples of pigs infected with classical swine ...	2015	25961582
the influence of antral ulcers on intramural gastric nerve projections supplying the pyloric sphincter in the pig (sus scrofa domestica)-neuronal tracing studies.	gastric ulcerations in the region of antrum pylori represent a serious medical problem in humans and animals. such localization of ulcers can influence the intrinsic descending nerve supply to the pyloric sphincter. the pyloric function is precisely regulated by intrinsic and extrinsic nerves. impaired neural regulation could result in pyloric sphincter dysfunction and gastric emptying malfunction. the aim of the study was to determine the effect of gastric antral ulcerations on the density and ...	2015	25962176
mitochondrial dna diversity of feral pigs from karukinka natural park, tierra del fuego island, chile.	control or eradication of exotic species is one of the greatest challenges facing biodiversity and ecosystem conservation. domestic pigs (sus scrofa domestica) were released and became feral in the southern region of chilean tierra del fuego island in the 1900s. currently, they inhabit part of karukinka natural park, an area of global conservation concern. to gain insight into the control of this invasive species, we analyzed genetic variation in the mitochondrial dna control region to determine ...	2015	25966196
a longitudinal survey of african swine fever in uganda reveals high apparent disease incidence rates in domestic pigs, but absence of detectable persistent virus infections in blood and serum.	african swine fever (asf) is a fatal, haemorrhagic disease of domestic pigs, that poses a serious threat to pig farmers and is currently endemic in domestic pigs in most of sub-saharan africa. to obtain insight into the factors related to asf outbreaks at the farm-level, a longitudinal study was performed in one of the major pig producing areas in central uganda. potential risk factors associated with outbreaks of asf were investigated including the possible presence of apparently healthy asf-vi ...	2015	25967670
early postnatal respiratory viral infection induces structural and neurochemical changes in the neonatal piglet brain.	infections that cause inflammation during the postnatal period are common, yet little is known about their impact on brain development in gyrencephalic species. to address this issue, we investigated brain development in domestic piglets which have brain growth and morphology similar to human infants, after experimentally infecting them with porcine reproductive and respiratory syndrome virus (prrsv) to induce an interstitial pneumonia piglets were inoculated with prrsv on postnatal day (pd) 7 a ...	2015	25967923
comparison of autologous (111)in-leukocytes, (18)f-fdg, (11)c-methionine, (11)c-pk11195 and (68)ga-citrate for diagnostic nuclear imaging in a juvenile porcine haematogenous staphylococcus aureus osteomyelitis model.	the aim of this study was to compare (111)in-labeled leukocyte single-photon emission computed tomography (spect) and (18)f-fluorodeoxyglucose ((18)f-fdg) positron emission tomography (pet) to pet with tracers that potentially could improve detection of osteomyelitis. we chose (11)c-methionine, (11)c-pk11195 and (68)ga-citrate and validated their diagnostic utility in a porcine haematogenous osteomyelitis model. four juvenile 14-15 weeks old female pigs were scanned seven days after intra-arteri ...	2015	25973338
when pigs fly, ucp1 makes heat.	brown and beige adipose tissue may represent important therapeutic targets for the treatment of diabetes and obesity as these organs dissipate nutrient energy as heat through the thermogenic uncoupling protein 1 (ucp1). while mice are commonly used to mimic the potential effects of brown/beige adipose tissue that may act in human metabolism, new animal models are edging into the market for translational medicine. pigs reflect human metabolism better than mice in multiple parameters such as obesi ...	2015	25973382
characterization of porcine parvovirus type 3 and porcine circovirus type 2 in wild boars (sus scrofa) in slovakia.	as the number of free-living wild boars (sus scrofa l.) continues to rise in slovakia, the probability of pathogen transmission between susceptible species increases. we investigated the distribution and genetic characterization of porcine parvovirus type 3 (ppv3), porcine circovirus type 2 (pcv2), and their coinfection in wild boars. among 194 animals tested, 19.1% were positive for ppv3 and 43.8% for pcv2. similar rates of coinfection with both viruses reaching 11.0% and 11.8% were observed in ...	2015	25973618
genetic characterisation of porcine circovirus type 2 (pcv2) strains from feral pigs in the brazilian pantanal: an opportunity to reconstruct the history of pcv2 evolution.	since its discovery, porcine circovirus type 2 has emerged as one of the most relevant swine infectious diseases, causing relevant economic losses for the pig industry. while four genotypes were identified, only three (pcv2a, pcv2b and pcv2d) are currently circulating and display a worldwide distribution. another genotype, pcv2c, has been described only once in danish archive samples collected between 1980 and 1990. in addition to commercial pigs, pcv2 has been demonstrated to infect wild boars ...	2015	25975522
dose-dependent vascular response following delivery of sirolimus via fast releasing, biodegradable polymer stent matrix: an experimental study in the porcine coronary model of restenosis.	fast releasing, rapamycin-eluting stents, although safe, showed inferior results with regard to inhibition of restenosis.	2015	25985728
experimental pig-to-pig transmission dynamics for african swine fever virus, georgia 2007/1 strain.	african swine fever virus (asfv) continues to cause outbreaks in domestic pigs and wild boar in eastern european countries. to gain insights into its transmission dynamics, we estimated the pig-to-pig basic reproduction number (r 0) for the georgia 2007/1 asfv strain using a stochastic susceptible-exposed-infectious-recovered (seir) model with parameters estimated from transmission experiments. models showed that r 0 is 2·8 [95% confidence interval (ci) 1·3-4·8] within a pen and 1·4 (95% ci 0·6- ...	2016	25989921
human microbiota-associated swine: current progress and future opportunities.	gnotobiotic (gn) rodent models have provided insight into the contributions of the gut microbiota to host health and preventing disease. however, rodent models are limited by several important physiological and metabolic differences from humans, and many rodent models do not dependably replicate the clinical manifestations of human diseases. due to the high degree of similarity in anatomy, physiology, immunology and brain growth, the domestic pig (sus scrofa) is considered a clinically relevant ...	2015	25991699
first detection of sarcoptes scabiei from domesticated pig (sus scrofa) and genetic characterization of s. scabiei from pet, farm and wild hosts in israel.	in this report we describe for the first time the detection of sarcoptes scabiei type suis mites on domestic pigs in israel and examine its genetic variation compared with s. sabiei from other hosts. microscopic examination of skin samples from s. scabiei-infested pigs (sus scrofa domesticus) revealed all developmental stages of s. scabiei. to detect genetic differences between s. scabiei from different hosts, samples obtained from pig, rabbits (orictolagus cuniculus), fox (vulpes vulpes), jacka ...	2015	26002310
a quantitative ethnozoological study of traditionally used animals in pachamalai hills of tamil nadu, india.	the purpose of this study was designed to gather primary folk knowledge on different animal based therapies used by malayalis in pachamalai hills. this is the first ethnozoological study in pachamalai hills; the data regarding the medicinal animals/animal products were documented and their usages were analyzed quantitatively.	2015	26002766
hepatitis e virus genotype 3 diversity: phylogenetic analysis and presence of subtype 3b in wild boar in europe.	an increasing number of indigenous cases of hepatitis e caused by genotype 3 viruses (hev-3) have been diagnosed all around the word, particularly in industrialized countries. hepatitis e is a zoonotic disease and accumulating evidence indicates that domestic pigs and wild boars are the main reservoirs of hev-3. a detailed analysis of hev-3 subtypes could help to determine the interplay of human activity, the role of animals as reservoirs and cross species transmission. although complete genome ...	2015	26008708
the synergy biodegradable polymer everolimus eluting coronary stent: porcine vascular compatibility and polymer safety study.	synergy is a novel platinum chromium alloy stent that delivers abluminal everolimus from an ultrathin poly-lactide-co-glycide (plga) biodegradable polymer. this study evaluated the in vivo degradation of the polymer coating, everolimus release time course, and vascular compatibility of the synergy stent.	2015	26009986
east african pigs have a complex indian, far eastern and western ancestry.	in this study, we have characterized the mitochondrial diversity of 81 swine from uganda. median-joining network analysis of d-loop sequences from these individuals and others characterized in previous studies allowed us to determine that ugandan pigs cluster with populations from the west (europe/north africa), far east and india. in addition, partial sequencing of the y-chromosome uty locus in 18 ugandan domestic pigs revealed the segregation of a single hy1 lineage that has a cosmopolitan dis ...	2015	26011180
chest compression synchronized ventilation versus intermitted positive pressure ventilation during cardiopulmonary resuscitation in a pig model.	guidelines recommend mechanical ventilation with intermitted positive pressure ventilation (ippv) during resuscitation. the influence of the novel ventilator mode chest compression synchronized ventilation (ccsv) on gas exchange and arterial blood pressure compared with ippv was investigated in a pig model.	2015	26011525
vegf-c and vegf-c156s in the pro-lymphangiogenic growth factor therapy of lymphedema: a large animal study.	vegf-c156s, a lymphangiogenesis-specific form of vascular endothelial growth factor c (vegf-c), has been considered as a promising candidate for the experimental pro-lymphangiogenic treatment, as it lacks potential angiogenic effects. as a precursor to future clinical trials, the therapeutic efficacy and blood vascular side effects of vegf-c and vegf-c156s were compared in a large animal model of secondary lymphedema. combination of lymphatic growth factor treatment and autologous lymph node tra ...	2015	26018927
carcass mass has little influence on the structure of gravesoil microbial communities.	little is known about how variables, such as carcass mass, affect the succession pattern of microbes in soils during decomposition. to investigate the effects of carcass mass on the soil microbial community, soils associated with swine (sus scrofa domesticus) carcasses of four different masses were sampled until the 15th day of decomposition during the month of june in a pasture near lincoln, nebraska. soils underneath swine of 1, 20, 40, and 50 kg masses were investigated in triplicate, as well ...	2016	26024793
prevalence and characterization of influenza viruses in diverse species in los llanos, colombia.	while much is known about the prevalence of influenza viruses in north america and eurasia, their prevalence in birds and mammals in south america is largely unknown. to fill this knowledge gap and provide a baseline for future ecology and epidemiology studies, we conducted 2 years of influenza surveillance in the eastern plains (los llanos) region of colombia. real-time reverse transcriptase polymerase chain reaction (rt-pcr) identified influenza viruses in wild birds, domestic poultry, swine a ...	2013	26038461
anaplasma sp. and hemoplasma infection in leopard cats (prionailurus bengalensis euptilurus) from korea.	this study examined the occurrence of anaplasma spp. and hemoplasma infection in leopard cats, prionailurus bengalensis euptilurus, in korea. twenty-nine biological samples were tested by molecular analysis. two (6.9%) and eight (27.6%) tested specimens were positive for anaplasma bovis and hemoplasma infection, respectively. based on our results, anaplasma/ehrlichia spp. and hemoplasma are regularly infecting leopard cat populations of korea. considering their endangered status, regular monitor ...	2015	26040618
intermediate veins in swine (sus scrofa domestica) kidney: authors' own anatomical classification.	the use of domestic swine as an experimental animal is increasing steadily. swine organs are the best animal model for urological experiments. the aim of the study was to evaluate the course and size of intermediate veins in a swine kidney. the research results were compared with the results obtained from studies on venous vascularisation of human kidneys. the knowledge of the above-mentioned vessels is important both in human and veterinary medicine and will enable researchers to compare and no ...	2015	26050812
evaluation of the zoonotic potential of a novel reassortant h1n2 swine influenza virus with gene constellation derived from multiple viral sources.	in 2011-2012, contemporary north american-like h3n2 swine influenza viruses (sivs) possessing the 2009 pandemic h1n1 matrix gene (h3n2pm-like virus) were detected in domestic pigs of south korea where h1n2 siv strains are endemic. more recently, we isolated novel reassortant h1n2 sivs bearing the eurasian avian-like swine h1-like hemagglutinin and korean swine h1n2-like neuraminidase in the internal gene backbone of the h3n2pm-like virus. in the present study, we clearly provide evidence on the ...	2015	26051886
[on the situation of african swine fever and the biological characterization of recent virus isolates].	african swine fever (asf), a disease notifiable to the world organization of animal health (oie), is characterized by severe, unspecific clinical signs and high mortality rates. hosts for asf virus (asfv) are only members of the family suidae and soft ticks of the genus ornithodoros. currently, no vaccine is available and therefore, the control is primarily based on strict sanitary measures. the most important part is the early detection of the disease within affected animal holdings and the fas ...	2015	26054220
detection of porcine circovirus type 2 and its association with pmws in wild boars and domestic pigs in germany: a histopathological, immunohistochemical and molecular biological study.	beside domestic pigs wild boars can also be affected by postweaning multisystemic wasting syndrome (pmws). for the first time a nationwide survey of wild boars (n = 356) and domestic pigs (n = 340) was carried out in germany by histopathology, immunohistochemistry (ihc) and quantitative pcr (qpcr). whereas 102/340 domestic pigs were immunoreactive for pcv2 antigen in at least one examined tissue, only 8/356 wild boars reacted positively. similar findings could be found in qpcr: all domestic pigs ...	2015	26054225
expansion of the hsfy gene family in pig lineages : hsfy expansion in suids.	amplified gene families on sex chromosomes can harbour genes with important biological functions, especially relating to fertility. the y-linked heat shock transcription factor (hsfy) family has become amplified on the y chromosome of the domestic pig (sus scrofa), in an apparently independent event to an hsfy expansion on the y chromosome of cattle (bos taurus). although the biological functions of hsfy genes are poorly understood, they appear to be involved in gametogenesis in a number of mamm ...	2015	26055083
genetic variation of the porcine nr5a1 is associated with meat color.	because of the central role of steroidogenic factor 1 in the regulation of the development and function of steroidogenic tissues, including the adrenal gland, we chose the encoding gene nr5a1 as a candidate for stress response, meat quality and carcass composition in the domestic pig. to identify polymorphisms of the porcine nr5a1 we comparatively sequenced the coding, untranslated and regulatory regions in four commercial pig lines. single nucleotide polymorphisms could be found in the 3' utr a ...	2016	26055431
african swine fever virus georgia 2007 with a deletion of virulence-associated gene 9gl (b119l), when administered at low doses, leads to virus attenuation in swine and induces an effective protection against homologous challenge.	african swine fever virus (asfv) is the etiological agent of an often lethal disease of domestic pigs. disease control strategies have been hampered by the unavailability of vaccines against asfv. since its introduction in the republic of georgia, a highly virulent virus, asfv georgia 2007 (asfv-g), has caused an epizootic that spread rapidly into eastern european countries. currently no vaccines are available or under development to control asfv-g. in the past, genetically modified asfvs harbor ...	2015	26063424