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use of plethysmographic variability index and perfusion index to evaluate changes in arterial blood pressure in anesthetized tigers (panthera tigris).objective to investigate use of the plethysmographic variability index (pvi) and perfusion index (pi) for evaluating changes in arterial blood pressure in anesthetized tigers (panthera tigris). animals 8 adult tigers. procedures each tiger was anesthetized once with a combination of ketamine, midazolam, medetomidine, and isoflurane. anesthetic monitoring included assessment of pi, pvi, direct blood pressure measurements, anesthetic gas concentrations, esophageal temperature, and results of capno ...201830058850
effects of human impacts on habitat use, activity patterns and ecological relationships among medium and small felids of the atlantic forest.competition theory and niche theory suggest that two morphologically similar species may coexist by reducing the overlap of at least one dimension of their ecological niche. the medium and small neotropical felids are an interesting group of carnivore species for studying intraguild competition. due to differences in size it is expected that the larger ocelot exert strong interference competition on the smaller felids (southern tiger cat, margay and jaguarundi); which, in turn, may exert exploit ...201830067785
rapid evolution and the genomic consequences of selection against interspecific mating.while few species introduced into a new environment become invasive, those that do provide critical information on ecological mechanisms that determine invasions success and the evolutionary responses that follow invasion. aedes albopictus (the asian tiger mosquito) was introduced into the naturalized range of aedes aegypti (the yellow fever mosquito) in the united states in the mid-1980s, resulting in the displacement of a. aegypti in much of the south-eastern united states. the rapid displacem ...201830069966
delivery and evaluation of participatory education for animal keepers led by veterinarians and para-veterinarians around the kanha tiger reserve, madhya pradesh, india.we aim to investigate local perceptions of animal health challenges; current animal health knowledge; and methods to provide effective, relevant education to animal keepers in the kanha tiger reserve area.201830071034
genetic and environmental influences on the size-fecundity relationship in aedes albopictus (diptera: culicidae): impacts on population growth estimates?population growth models are integral to ecological studies by providing estimates of population performance across space and time. several models have been developed that estimate population growth through correlates of demographic traits, as measuring each parameter of the model can be prohibitive in experimental studies. since differences in female size can accurately reflect changes in fecundity for many taxa, livdahl and sugihara developed a population growth index that incorporates size-fe ...201830071049
identification of micrornas and their target genes associated with ovarian development in black tiger shrimp (penaeus monodon) using high-throughput sequencing.plenty of evidence showing that micrornas (mirnas) post-transcriptionally regulate gene expression and are involved in a wide range of biological processes. however, the roles of mirnas in ovarian development process remain largely unknown in shrimp. in the present study, high-throughput sequencing of small rnas was performed to find specific mirnas that are involved in ovarian development process in penaeus monodon. two small rna libraries were constructed from undeveloped (undev group) and dev ...201830072718
attractant or repellent? behavioral responses to mammalian blood odor and to a blood odor component in a mesopredator, the meerkat (suricata suricatta).it is well-established that the odor of mammalian blood is attractive to top predators such as tigers and wolves and aversive to prey species such as mice and rats. recent studies have shown that the mammalian blood odor component trans-4,5-epoxy-(e)-2-decenal (ted) elicits corresponding behavioral responses in these two groups of mammals. here we assess whether a mesopredator, that is, a small-bodied carnivorous mammal that is both predator and prey, is attracted to or repelled by the odor of m ...201830083096
land use and larval habitat increase aedes albopictus (diptera: culicidae) and culex quinquefasciatus (diptera: culicidae) abundance in lowland hawaii.vector abundance plays a key role in transmission of mosquito-borne disease. in hawaii, aedes albopictus (skuse) (diptera: culicidae), the asian tiger mosquito, has been implicated in locally-transmitted dengue outbreaks, while culex quinquefasciatus say (diptera: culicidae), the southern house mosquito, is the primary vector of avian malaria, a wildlife disease that has contributed to declines and extinctions of native hawaiian birds. despite the importance of these introduced species to human ...201830085189
laparoscopic-assisted ovariohysterectomy for the treatment of pyometra in a bengal tiger (panthera tigris tigris).a laparoscopic-assisted ovariohysterectomy was performed in a 19-year-old intact, female bengal tiger (panthera tigris tigris) presented for surgical treatment of pyometra. a multi-port technique was used with intra-corporeal sealing of the ovarian pedicles and extra-corporeal ligation of the uterine vessels and body. the tiger recovered from surgery and anesthesia without complication, was released into its enclosure the same day, and has remained clinically normal. laparoscopic-assisted ovario ...201830104782
a morphological study of the male reproductive tract, post-testicular acrosome maturation and spermatophore formation in the black tiger prawn (penaeus monodon).inferior larval production rates of domesticated penaeus monodon broodstock has been a major hurdle to the expansion of its aquaculture, so that a better understanding of basic male reproductive biology is critical to improve the reproductive performance of this commercially important penaeid species. following our previous study of spermatogenesis in the species, this study explored the mechanism of spermatophore formation with regards to the contribution of the reproductive tract epithelium by ...201830117606
the functional anatomy of external genitalia in the black tiger prawn (penaeus monodon) studied by micro-computed tomography and scanning electron microscopy.although artificial insemination has been used for decades in penaeus monodon aquaculture, the interaction of male and female external genitalia during spermatophore transfer has not been fully documented. as a result, studying the functional anatomy of this process may help to better refine the insemination technique. the sexual act in penaeoid prawns is virtually impossible to observe directly; as a result, this study aimed to describe the functional anatomy and interaction of external genital ...201830117611
bioinformatic analysis of endogenous and exogenous small rnas on lipoproteins.to comprehensively study extracellular small rnas (srna) by sequencing (srna-seq), we developed a novel pipeline to overcome current limitations in analysis entitled, "tools for integrative genome analysis of extracellular srnas (tiger)". to demonstrate the power of this tool, srna-seq was performed on mouse lipoproteins, bile, urine and livers. a key advance for the tiger pipeline is the ability to analyse both host and non-host srnas at genomic, parent rna and individual fragment levels. tiger ...201830128086
species, sex and geo-location identification of seized tiger (panthera tigris tigris) parts in nepal-a molecular forensic approach.tiger (panthera tigris) populations are in danger across their entire range due to habitat loss, poaching and the demand for tiger parts. the bengal tiger (panthera tigris tigris) is an endangered apex predator with a population size estimated to be less than 200 in nepal. in spite of strict wildlife protection laws, illegal trade of tiger parts is increasing; and nepal has become one of the major sources and transit routes for poached wildlife parts. identification of wildlife parts is often ch ...201830138352
the asian tiger mosquito aedes albopictus (diptera: culicidae) in central germany: surveillance in its northernmost distribution area.the invasive asian tiger mosquito aedes albopictus has recently been observed in southern germany for the first time to reproduce and even overwinter north of the alps. after the accidental capture of adult specimens in jena, german federal state of thuringia, in mid-2015, regular inspections brought forth developmental stages until autumn 2015, indicating local reproduction. surveillance activities implemented in 2016 showed larvae already in early may, suggesting overwintering, and throughout ...201830145257
ambystoma mavortium (barred tiger salamander). behavior underneath the ice. 201330147128
correction: delivery and evaluation of participatory education for animal keepers led by veterinarians and para-veterinarians around the kanha tiger reserve, madhya pradesh, india.[this corrects the article doi: 10.1371/journal.pone.0200999.].201830148892
addressing human-tiger conflict using socio-ecological information on tolerance and risk.tigers are critically endangered due to deforestation and persecution. yet in places, sumatran tigers (panthera tigris sumatrae) continue to coexist with people, offering insights for managing wildlife elsewhere. here, we couple spatial models of encounter risk with information on tolerance from 2386 sumatrans to reveal drivers of human-tiger conflict. risk of encountering tigers was greater around populated villages that neighboured forest or rivers connecting tiger habitat; geographic profiles ...201830150649
the invasive mosquito aedes albopictus (diptera, culicidae) firstly recorded in bohemia, czech republic.in 2016-2017, the monitoring of possible introduction of an invasive mosquito species, the asian tiger mosquito aedes (stegomyia) albopictus (skuse, 1894) (diptera, culicidae), was conducted in eastern, southern, central and western parts of bohemia, czech republic.201930157658
diagnosis and treatment of lower motor neuron disease in australian dogs and cats.diseases presenting with lower motor neuron (lmn) signs are frequently seen in small animal veterinary practice in australia. in addition to the most common causes of lmn disease seen world-wide, such as idiopathic polyradiculoneuritis and myasthenia gravis, there are several conditions presenting with lmn signs that are peculiar to the continent of australia. these include snake envenomation by tiger (notechis spp.), brown (pseudonaja spp.), and black snakes (pseudechis spp.), tick paralysis as ...201830159335
characterizing the influence of metabolism on the halogenated organic contaminant biomagnification in two artificial food chains using compound- and enantiomer-specific stable carbon isotope analysis.two artificial food chains, food tiger barb-oscar fish and food tiger barb-redtail catfish, were established in the laboratory. the species-specific biotransformation of ortho, para'-dichlorodiphenyltrichloroethane, 12 polychlorinated biphenyl, and five polybrominated diphenyl ether congeners were characterized by measuring the compound- and enantiomer-specific stable carbon isotope composition (δ13c), enantiomeric fraction of the chiral chemicals, and metabolites in the fish. compound- and enan ...201830160487
a simple method for data partitioning based on relative evolutionary rates.multiple studies have demonstrated that partitioning of molecular datasets is important in model-based phylogenetic analyses. commonly, partitioning is done a priori based on some known properties of sequence evolution, e.g. differences in rate of evolution among codon positions of a protein-coding gene. here we propose a new method for data partitioning based on relative evolutionary rates of the sites in the alignment of the dataset being analysed. the rates are inferred using the previously p ...201830186687
ingestion of inorganic mercury by juvenile black tiger prawns (penaeus monodon) alters biochemical markers.there is a lack of information regarding the effects on biochemical markers in invertebrates diet-exposed to inorganic mercury. in the present study, juvenile black tiger prawn (penaeus monodon) were fed with food dosed with mercuric chloride (low: 0.2 mg kg-1; medium: 0.77 mg kg-1; high: 1.41 mg kg-1; higher: 2.52 mg kg-1) and control (0.03 mg kg-1) over 12 days. at the end of exposure periods, ventral nerve cord, compound eyes and muscle were dissected for biochemical marker analyses (acetylch ...201830187358
plant-derived compounds regulate formation of the insect juvenile hormone receptor complex.insect growth regulators (igrs) are attractive pest control agents due to their high target specificity and relative safety to the environment. recently, plants have been shown to synthesize igrs that affect the insect juvenile hormone (jh) as a part of their defense mechanisms. using a yeast two-hybrid system transformed with the aedes aegypti jh receptor as a reporter system, we identified several jh agonists (jhas) and antagonists (jhans) causing retardation in the ovarian development of fema ...201830195384
counter-urbanisation during ireland's 'celtic tiger' period - mental health implications.this study (a) describes the clinical and demographic profile of urban to rural migrants attending a psychiatric outpatient clinic and (b) explores the impact of the move on patients' mental health and lifestyle.201130200020
de novo assembly, characterization, functional annotation and expression patterns of the black tiger shrimp (penaeus monodon) transcriptome.the black tiger shrimp (penaeus monodon) remains the second most widely cultured shrimp species globally; however, issues with disease and domestication have seen production levels stagnate over the past two decades. to help identify innovative solutions needed to resolve bottlenecks hampering the culture of this species, it is important to generate genetic and genomic resources. towards this aim, we have produced the most complete publicly available p. monodon transcriptome database to date bas ...201830202061
transcriptome reveals involvement of immune defense, oxidative imbalance, and apoptosis in ammonia-stress response of the black tiger shrimp (penaeus monodon).ammonia is a major aquatic environmental pollutant that negatively impacts shrimp health and commercial productivity. however, we currently do not fully understand the underlying molecular mechanisms of ammonia stress in shrimp. we therefore performed transcriptomic analysis of hepatopancreas from black tiger shrimp (penaeus monodon) treated with ammonia-stress. we obtained 146,410,174 and 115,241,048 clean reads for the control and treatment groups, respectively. a total of 64,475 unigenes with ...201830205201
nutrient evaluation of a pork by-product and its use as environmental enrichment for managed large exotic cats.north american zoological institutions typically feed ground raw meat diets to large exotic cats. these diets typically are nutritionally complete, but lack physical properties characteristic of whole prey. lack of mastication and prey manipulation may contribute to behavioral and health challenges. pork by-products may provide environmental enrichment to mitigate these challenges. the objectives of this study were to evaluate a pig head for nutritional composition and to determine if a pig head ...201830208036
gastric dilatation and enterotoxemia in ten captive felids.case description 10 large felids at 8 facilities were determined or suspected to have developed gastric dilatation with or without enterotoxemia over a 20-year period. four felids were found dead with no premonitory signs. clinical findings 4 felids (2 male snow leopards [uncia uncia], 1 male amur tiger [panthera tigris altaica], and 1 male sumatran tiger [panthera tigris sumatrae]) were found dead or died before they could be evaluated. six felids had hematemesis (1 male and 1 female african li ...201830211645
performance of one- compared with two-catheter concepts in transradial coronary angiography (from the randomized use of different diagnostic catheters-radial-trial).the use of different diagnostic catheters-radial-trial sought to compare the safety and efficacy of one-catheter concepts (occ) using tiger ii or blk catheters with two-catheter concepts (tcc) using standard judkins catheters for transradial coronary angiography. a total of 300 patients planed for coronary angiography were enrolled into this single-center, single-blinded trial. patients were randomized in a 2:1 ratio to either occ by tiger ii (n = 100) and blk (n = 100) or tcc by judkins (n = 10 ...201830217374
left atrial appendage-beyond being a thrombus-spreading tiger. 201830225533
characterizing conflict between humans and big cats panthera spp: a systematic review of research trends and management opportunities.conservation of big cats (panthera spp.), a taxonomic group including tigers, lions, jaguars, leopards and snow leopards, is a daunting challenge. as expanding human populations across panthera range countries exacerbate competition for land and prey, conflicts between humans and big cats are inevitable. through a systematic review of the peer-reviewed literature published from 1991 to 2014 and indexed in web of science and google scholar (186 articles), our study explored the current state of k ...201830226894
clinical effect of a mutation (p.glu322asp, c.966 g>t) in pank2 gene in a family with atypical pantothenate kinase-associated neurodegeneration.pantothenate-kinase-associated neurodegeneration (pkan) is a rare autosomal recessive disorder caused by mutations in the pantothenate kinase 2 (pank2) gene. many different mutations in the pank2 gene have been detected in association with pkan. a 20 year old female patient who had been suffering from progressive gait disorder for 1 year was found to have the 'eye-of-the-tiger sign' from the brain magnetic resonance imaging (mri). the same brain imaging findings were shown in the father and brot ...201830226968
management of refractory germ cell cancer.over the past 5 decades, the use of well-validated, guideline-based strategies has resulted in high cure rates in newly diagnosed patients with germ cell cancer. however, about 30% of those with metastatic disease at initial presentation will experience refractory disease. salvage treatment is far more complex and less validated than first-line treatment because it is rare, patient cohorts are more heterogeneous, and prognostic factors seem to have greater impact. prior to the initiation of any ...201830231348
autosomal-recessive iron deficiency anemia, dystonia and hypermanganesemia caused by new variant mutation of the manganese transporter gene slc39a14.this inborn error of manganese metabolism has only recently been identified. a total of 28 affected individuals from ten families are known worldwide. mutations in slc39a14, encoding a mn uptake transporter, have recently been recognized to cause excessive mn concentrations in the blood which is believed to be neurotoxic and lead to a parkinsonian-like movement disorder caused by accumulation of mn in the basal ganglia. we are reporting a new variant of slc39a14 gene mutation (omim 608736 8p21.3 ...201930232769
nutritional evaluation of processing discards from tiger tooth croaker, otolithes ruber.processing discards from tiger tooth croaker, such as head, viscera, and swim bladder, were analyzed for proximate, biochemical, fatty acid, and mineral composition. the proximate analysis showed high protein content (21.04%) in the swim bladder and high fat content (4.10%) and ash content (2.26%) in the head. the biochemical quality indices such as non-protein nitrogen, total volatile base, trimethylamine, free fatty acids, peroxide value, and thiobarbituric acid reactive substances in the visc ...201630263402
high fibre tiger nut pasta and xanthan gum: cooking quality, microstructure, physico-chemical properties and consumer acceptance.the feasibility of replacing wheat semolina by tiger nut flour (20 and 40%) and xanthan gum (1%) in order to obtain high fibre dry pappardelle with fair techno-functional, structural and sensory attributes, was assessed. the cooking properties, texture, colour and sensory acceptance of uncooked and cooked pasta were evaluated. the proximate chemical composition of the raw materials, and the microstructure of the dry pasta were also assessed. the results in this manuscript address the improved nu ...201830263837
encoding actions and verbs: tracking the time-course of relational encoding during message and sentence formulation.two experiments tracked the encoding of relational information (actions at the level of the prelinguistic message and verbs at the level of the sentence) during formulation of transitive event descriptions (e.g., the tiger is scratching the photographer). at what point during message and sentence formulation do speakers encode actions and verbs? participants described pictures of transitive events in response to neutral questions ("what is happening?"), agent questions ("what is [the agent] doin ...201930265052
new records of sharks (elasmobranchii) from the andaman and nicobar archipelago in india with notes on current checklists.the diversity of sharks occurring off the andaman and nicobar archipelago in india has received increased attention in recent years. yet, available checklists are out of date, often with inaccurate information and a number of commercially important species have not been documented through research and fish landing surveys. here we report on shark species examined during fish landing surveys conducted from january 2017 to april 2018. records of twelve previously unreported species from the archip ...201830271254
enhancing bioactive antioxidants' extraction from "horchata de chufa" by-products.during the production of a traditional drink produced from the tubers of cyperus esculentus l. also known as "horchata de chufa," a high quantity of by-products are generated. these by-products are rich with valuable biological compounds, hence, there is a need to report their extraction conditions for further use in food production as raw materials. therefore, the objective of this study was to evaluate and improve the conventional extraction process, applied for recovery of phenolic compounds, ...201830275430
cell-free dna next-generation sequencing prediction of response and resistance to third-generation egfr inhibitor.the genomic alterations driving resistance to third-generation egfr tyrosine kinase inhibitors (tkis) are not well established, and collecting tissue biopsy samples poses potential complications from invasive procedures. cell-free circulating dna (cfdna) testing provides a noninvasive approach to identify potentially targetable mechanisms of resistance. here we utilized a 70-gene cfdna next-generation sequencing test to interrogate pretreatment and progression samples from 77 egfr-mutated non-sm ...201830279111
long-distance seed dispersal affects the resilience of banded vegetation patterns in semi-deserts.landscape-scale vegetation stripes (tiger bush) observed on the gentle slopes of semi-arid regions are useful indicators of future ecosystem degradation and catastrophic shifts towards desert. mathematical models like the klausmeier model-a set of coupled partial differential equations describing vegetation and water densities in space and time-are central to understanding their formation and development. one assumption made for mathematical simplicity is the local dispersal of seeds via a diffu ...201930292800
thermoplastic starch/polybutylene adipate terephthalate film coated with gelatin containing nisin z and lauric arginate for control of foodborne pathogens associated with chilled and frozen seafood.in order to control foodborne pathogens on seafood products, an antimicrobial, thermoplastic starch/polybutylene adipate terephthalate (tps/pbat; 40/60) film was produced by coating gelatin (15% v/v) containing lauric arginate (lae; 0.8 mg/cm2), alone or combination with nisin z (69.4 au/cm2) to produce lae-gelatin-tps/pbat and nisin-lae-gelatin-tps/pbat films, respectively. both films were investigated for control of vibrio parahaemolyticus atcc 17802 and salmonella typhimurium atcc 14028 on bi ...201930296637
genomic data recover previously undetectable fragmentation effects in an endangered amphibian.a critical consideration when using molecular ecological methods to detect trends and parameterize models at very fine spatial and temporal scales has always been the technical limits of resolution. key landscape features, including most anthropogenic modifications, can cause biologically important, but very recent changes in gene flow that require substantial statistical power to detect. the problem is one of temporal scale: human change is rapid and recent, while genetic changes accumulate slo ...201830307076
the feeding system of terrestrial tiger salamanders (ambystoma tigrinum melanostictum baird).high speed cinematography was used to record the feeding activities of terrestrial ambystoma tigrinum melanostictum. a description of these activities based on films of more than 50 feeding sequences is presented, and the mechanical units involved are defined, described, and functionally analyzed. evolutionary implications of the feeding system are discussed. in a typical feeding sequence, a. t. melanostictum stations and maintains its lower jaw 3-5 mm from the prey. the mouth is then opened to ...197530309060
targeting restoration sites to improve connectivity in a tiger conservation landscape in india.maintaining and restoring connectivity between source populations is essential for the long term viability of wide-ranging species, many of which occur in landscapes that are under pressure to meet increasing infrastructure needs. identifying barriers in corridors can help inform conservation and infrastructure development agencies so that development objectives can be achieved without compromising conservation goals. here, we use the tiger landscape in central india as a case study to identify ...201830310737
construction of an efficient genomic editing system with crispr/cas9 in the vector mosquito aedes albopictus.aedes (stegomyia) albopictus, also known as the asian tiger mosquito, is a mosquito which originated in asia. in recent years, it has become increasingly rampant throughout the world. this mosquito can transmit several arboviruses, including dengue, zika and chikungunya viruses, and is considered a public health threat. despite the urgent need of genome engineering to analyze specific gene functions, progress in genetical manipulation of ae. albopictus has been slow due to a lack of efficient me ...201930311353
erratum: jiří moravec jan vybíra  (2018) new or rare madagascar tiger beetles-17. description of pogonostoma (microstenocera) noheli sp. nov. and redescription of p. (m.) flavomaculatum w. horn (coleoptera: cicindelidae). zootaxa, 4388 (1): 076-088. 201830313183
a new species of genus neoaloa singh kirti, 2015 from bihar, india (lepidoptera: erebidae: arctiinae: arctiini).a new species, neoaloa cernyi singh joshi, sp. nov. is described from gular-ghat of valmiki tiger reserve, bihar, india. additionally, the original combination of aloa collaris hampson, 1891, comb. rev. is restored.201830313761
the only way is up for bengal tigers in nepal.georgina mills reports on how initiatives and collaborations have led to an increase in tiger numbers in nepal.201830315054
dynamics of fatty acid regulatory genes during ovarian development in penaeus monodon.the delay in ovarian maturation in farmed black tiger shrimp penaeus monodon has resulted in the widespread practice of feeding broodstock with the polychaete perinereis nuntia and their unilateral eyestalk ablation. although this practice alters fatty acid content in shrimp ovaries and hepatopancreas, its effects on fatty acid regulatory genes are yet to be systematically examined. here, microarray analysis was performed on hepatopancreas and ovary cdna collected from p. monodon at different ov ...201830328347
a new species of hypoprepia from the mountains of central arizona (lepidoptera, erebidae, arctiinae, lithosiini).a new firefly-mimicking lichen moth of the genus hypoprepia, h.lampyroides palting & ferguson, sp. n., is described from the mountains of east-central arizona and the sierra madre occidental of mexico. hypoprepia hübner, 1831 is a north american genus of lithosiine tiger moths, previously consisting of five species: h.fucosa hübner, 1831 and h.miniata (kirby, 1837), both of eastern and central north america; h.cadaverosa strecker, 1878 from the rocky mountains into new mexico and west texas; h.i ...201830337821
diabetic kidney disease: the tiger may have new stripes. 201830343731
neighbors help neighbors control urban mosquitoes.the worldwide spread of invasive aedes mosquitoes and arboviral disease, have renewed the pressure for effective and sustainable urban mosquito control. we report on the success of a model we are confident will usher in a new era of urban mosquito control. the key innovation is the mobilization of neighbors guided by scientific advisors, an approach we termed citizen action through science (citizen acts). this approach was tested in a ne us town of approximately 1,000 residential yards infested ...201830361483
detection of tiger podoplanin using the anti-cat podoplanin monoclonal antibody pmab-52.podoplanin (pdpn) is expressed in type i alveolar cells of lung but not in type ii alveolar cells. pdpn is also known as a specific lymphatic endothelial cell marker because pdpn is not expressed in vascular endothelial cells. pdpns of several animals have been characterized using specific anti-pdpn monoclonal antibodies (mabs): pmab-1, pmab-2, pmab-32, pmab-38, pmab-44, and pmab-52 for mouse, rat, rabbit, dog, bovine, and cat pdpns, respectively. in this study, we investigated the possible cros ...201830362928
pantothenate kinase-associated neurodegeneration (pkan) in a child with down syndrome. a case report and follow-up with mri.neurodegeneration with brain iron accumulation (nbia) is a subtype of inherited metabolic disorders. it includes pantothenate kinase-associated neurodegeneration (pkan), which is a rare autosomal recessive disorder caused by the mutation of pantothenate kinase 2-gene (pank2). it affects the deep grey matter nuclei causing progressive extrapyramidal motor impairment.1 we present a case of genetically proven pkan in a child with mosaic trisomy 21 in which t2- hyperintensity of the basal ganglia wa ...201530363610
potential of bacteriocins produced by probiotic bacteria isolated from tiger shrimp and prawns as antibacterial to vibrio,  pseudomonas, and aeromonas species on fish.backgrounds: bacteriocin has been used widely in industry as a biopreservative agent. the objective of the present study was to investigate the potency of bacteriocin isolated from tiger prawn penaeus monodon and freshwater shrimp macrobrachium rosenbergii as an anti-bacterial on fish. methods: a total of ten candidates of probiotic bacteria consisted of five isolates from tiger shrimps (h1, h2, h3, h4, h5) and five isolates from freshwater prawns (w1, w2, w3, w4, w5) were evaluated. bacteriocin ...201830363877
evaluation of baseline haemolymph biochemistry, volume and total body energetics to determine an accurate condition index in the black tiger shrimp, penaeus monodon.crustaceans are exposed to a range of environmental factors that can impact their condition, physiological function and growth. condition indices are broadly defined as the extent to which stored nutrient reserves allow normal physiological function and growth, but can also represent more than nutrition alone. there is currently no reliable indicator to non-destructively measure shrimp physiological or nutritional condition. in this study, haemolymph and biochemical indices were benchmarked agai ...201930366056
clinical perspective on dysplastic gangliocytoma of the cerebellum (lhermitte-duclos disease).dysplastic gangliocytoma of the cerebellum, also called lhermitte-duclos disease (ldd), is known as a rare, benign brain tumor. around 200 cases have been reported.201930366139
mapping and predicting forest loss in a sumatran tiger landscape from 2002 to 2050.riau province in central sumatra, with its peatland, lowland, and montane forest habitats, was once a stronghold for sumatran tiger (panthera tigris sumatrae) populations. today, riau may have one of the highest deforestation rates in the world and wildlife populations are dwindling, with natural forest now comprising approximately only 18% of the province, mostly contained within protected areas. agriculture (acacia, rubber, and oil palm) makes up the majority of riau's land cover and deforesta ...201930368149
gonadal response of juvenile protogynous grouper (epinephelus fuscoguttatus) to long-term recombinant follicle-stimulating hormone administration†.the role of follicle-stimulating hormone (fsh) in the gonadal development of protogynous hermaphroditic grouper (epinephelus fuscoguttatus) was investigated. recombinant giant grouper (e. lanceolatus) fsh (rggfsh) was produced in yeast. its receptor-binding capacity and steroidogenic potency were confirmed in vitro. weekly injections of rggfsh to juvenile tiger grouper for 8 weeks (100 μg/kg body weight, bw) resulted in significantly larger and more advanced oocytes (cortical alveolar stage vs p ...201930371741
acoustic analysis of voice in school teachers.the term 'voice' is the acoustic energy generated from the vocal tract that are characterized by their dependence on vocal fold vibratory pattern. teachers as professional voice users are afflicted with dysphonia and are discouraged with their jobs and seek alternative employment. loud speaking and voice straining may lead to vocal fatigue and vocal fold tissue damage. the objective of the study is to assess the quality of voice of school teachers before and after teaching practice.201930381759
diapause-associated changes in the lipid and metabolite profiles of the asian tiger mosquito, aedes albopictus.diapause is an alternative life-history strategy that allows organisms to enter developmental arrest in anticipation of unfavorable conditions. diapause is widespread among insects and plays a key role in enhancing overwinter survival as well as defining the seasonal and geographic distributions of populations. next-generation sequencing has greatly advanced our understanding of the transcriptional basis for this crucial adaptation but less is known about the regulation of embryonic diapause phy ...201830385483
the complete mitochondrial genome of parantica sita sita (lepidoptera: nymphalidae: danainae) revealing substantial genetic divergence from its sibling subspecies p. s. niphonica.currently, there are two subspecies of the chestnut tiger butterfly (parantica sita) recognized in china. p. s. sita is widely distributed in southwest china and p. s. niphonica in taiwan. periodically, taiwan island and the chinese mainland have been connected and separated because of sea level changes caused by pleistocene glaciations, most likely influencing the genetic structure among p. sita populations on both sides of the taiwan strait. also, p. s. niphonica's well-documented long-distanc ...201930391439
magnetic resonance imaging, susceptibility weighted imaging and quantitative susceptibility mapping findings of pantothenate kinase-associated neurodegeneration.pantothenate kinase-associated neurodegeneration (pkan) is extremely rare. in this study, we aimed to evaluate the magnetic resonance imaging (mri) findings of pkan patients. conventional mri and susceptibility weighted imaging (swi) sequences and quantitative susceptibility mapping (qsm) maps of six patients from three pkan families and eight healthy male volunteers were retrospectively analyzed. iron content was represented by qsm values. the typical eye-of-the-tiger sign (n = 4) and hyperinte ...201930392836
state space and movement specification in open population spatial capture-recapture models.with continued global changes, such as climate change, biodiversity loss, and habitat fragmentation, the need for assessment of long-term population dynamics and population monitoring of threatened species is growing. one powerful way to estimate population size and dynamics is through capture-recapture methods. spatial capture (scr) models for open populations make efficient use of capture-recapture data, while being robust to design changes. relatively few studies have implemented open scr mod ...201830397470
amphibians.since the dawn of history, amphibians have been a part of human culture. western europeans built fires for cooking and warmth, adding large logs as needed. what occasionally emerged was astounding: large black animals (which had found shelter in the logs) with four legs and a tail, jet black with striking bright yellow spots. these fire salamanders were variously thought to be the product of the fire itself, or, as aristotle reported, capable of extinguishing fire. pliny the elder is said to hav ...201830399342
corrigendum: phytoplasmas-the "crouching tiger" threat of australian plant pathology.[this corrects the article doi: 10.3389/fpls.2017.00599.].202030402053
recovery planning towards doubling wild tiger panthera tigris numbers: detailing 18 recovery sites from across the range.with less than 3200 wild tigers in 2010, the heads of 13 tiger-range countries committed to doubling the global population of wild tigers by 2022. this goal represents the highest level of ambition and commitment required to turn the tide for tigers in the wild. yet, ensuring efficient and targeted implementation of conservation actions alongside systematic monitoring of progress towards this goal requires that we set site-specific recovery targets and timelines that are ecologically realistic. ...201830408090
spop: age-structured discrete-time population dynamics model in c, python, and r.this article describes the spop packages implementing the deterministic and stochastic versions of an age-structured discrete-time population dynamics model. the packages enable mechanistic modelling of a population by monitoring the age and development stage of each individual. survival and development are included as the main effectors and they progress at a user-defined pace: follow a fixed rate, delay for a given time, or progress at an age-dependent manner. the model is implemented in c, py ...201830410730
advanced dietary patterns analysis using sparse latent factor models in young adults.principal components analysis (pca) has been the most widely used method for deriving dietary patterns to date. however, pca requires arbitrary ad hoc decisions for selecting food variables in interpreting dietary patterns and does not easily accommodate covariates. sparse latent factor models can be utilized to address these issues.201830418566
alien species and human health: austrian stakeholder perspective on challenges and solutions.no saturation in the introduction, acceleration of spread and the increasing impacts of alien species are a characteristic feature of the anthropocene. concomitantly, alien species affecting human health are supposed to increase, mainly due to increasing global trade and climate change. in this study, we assess challenges and solutions posed by such species to the public health sector in austria over the next few decades. we did so using an online questionnaire circulated to 131 experts and stak ...201830424500
empirical dynamic modelling identifies different responses of aedes polynesiensis subpopulations to natural environmental variables.to control mosquito populations for managing vector-borne diseases, a critical need is to identify and predict their response to causal environmental variables. however, most existing attempts rely on linear approaches based on correlation, which cannot apply in complex, nonlinear natural systems, because correlation is neither a necessary nor sufficient condition for causation. applying empirical dynamic modelling that acknowledges nonlinear dynamics on nine subpopulations of tiger mosquitos fr ...201830425277
tolerating tigers: gaining local and spiritual perspectives on human-tiger interactions in sumatra through rural community interviews.religious beliefs and spiritual connections to biodiversity have the potential to reduce animosity towards wildlife that might otherwise present a real or perceived threat to local people. understanding this social dynamic can therefore be important for formulating locally-appropriate species-specific conservation strategies. using semi-structured interviews which incorporated human-tiger conflict scenarios, we investigated how beliefs towards tigers varied between ethnic groups living around a ...201830427846
a membraneless organelle associated with the endoplasmic reticulum enables 3'utr-mediated protein-protein interactions.approximately half of human genes generate mrnas with alternative 3' untranslated regions (3'utrs). through 3'utr-mediated protein-protein interactions, alternative 3'utrs enable multi-functionality of proteins with identical amino acid sequence. while studying how information on protein features is transferred from 3'utrs to proteins, we discovered that the broadly expressed rna-binding protein tis11b forms a membraneless organelle, called tis granule, that enriches membrane protein-encoding mr ...201830449617
serological surveillance of avian influenza virus and canine distemper virus in captive siberian tigers in northeastern china.in order to understand infection of avian influenza a virus (aiv) and canine distemper virus (cdv) in the siberian tiger in northeast china, 75 siberian tiger serum samples from three cap- tive facilities in northeastern china were collected. aiv and cdv antibody surveillance was test- ed by using hemagglutination inhibition and serum neutralization methods. the results showed that the seroprevalence of h5 aiv, h9 aiv and cdv was respectively 9.33% (7/75), 61.33% (46/75) and 16% (12/75). in the ...201830468333
redescriptions, lectotype designations, new synonyms and new geographic records for the "tiger" species of mycotretus lacordaire, 1842 (coleoptera: erotylidae: tritomini).the neotropical mycotretus lacordaire, 1842 is one of the largest and most widespread genera of the erotylidae, encompassing more than 200 described species. among the species with a similar body coloration, there is a "group" of six valid species-called here the "tiger" mycotretus-that possess several pronotal and elytral black spots, as follows: m. tigrinus (olivier, 1792); m. multimaculatus taschenberg, 1870; m. centralis arrow, 1909; m. tigrinoides mader, 1942; m. tigripennis mader, 1942; an ...201830469485
protein trafficking through tiger domains. 201930479375
genome-wide evolutionary analysis of natural history and adaptation in the world's tigers.no other species attracts more international resources, public attention, and protracted controversies over its intraspecific taxonomy than the tiger (panthera tigris) [1, 2]. today, fewer than 4,000 free-ranging tigers survive, covering only 7% of their historical range, and debates persist over whether they comprise six, five, or two subspecies [3-6]. the lack of consensus over the number of tiger subspecies has partially hindered the global effort to recover the species from the brink of exti ...201830482605
a new species of freshwater crab of the genus teretamon yeo ng, 2007 (decapoda: brachyura: potamidae) from arunachal pradesh, northeastern india.a new species of potamid crab of the genus teretamon yeo ng, 2007, is described from namdapha tiger reserve in changlang district of arunachal pradesh, india. the new species, t. kempi n. sp., can be distinguished from its congeners by a distinct combination of carapace and gonopod characters: a relatively high carapace with a bilobed frontal margin; subquadrate sixth abdominal somite with nearly parallel lateral margins; and a relatively small g1 terminal segment with a semicircular to bluntly ...201830486053
a scoping review of published literature on chikungunya virus.chikungunya virus (chikv) has caused several major epidemics globally over the last two decades and is quickly expanding into new areas. although this mosquito-borne disease is self-limiting and is not associated with high mortality, it can lead to severe, chronic and disabling arthritis, thereby posing a heavy burden to healthcare systems. the two main vectors for chikv are aedes aegypti and aedes albopictus (asian tiger mosquito); however, many other mosquito species have been described as com ...201830496207
catch fast and kill quickly: do tiger beetles use the same strategies when hunting different types of prey?tiger beetles (coleoptera: cicindelidae) are fast running predatory insects preying on different small insects and other terrestrial arthropods. prey is located by sight and captured after short and fast pursuit interspersed with pause-and-look behaviour. at least some tiger beetle species can recognise the size and location of prey using memory, which probably allows them to achieve greater hunting success.201830498639
audit of toxic effects of body paint in the tiger dancers (hulivesha) of mangalore, india: an investigational study.tiger dancing or hulivesha, where the volunteers paint their bare body like a tiger and dance in a ritual during the navarathri festival in mangalore, india. there are no scientific studies done with the hulivesha dancers at all, and therefore, we investigated the adverse effects of painting body like a tiger and dancing in the volunteers.201930501442
metagenomic analysis of captive amur tiger faecal microbiome.the gastrointestinal tracts of animals are home to large, complex communities of microbes. the compositions of these communities ultimately reflect the coevolution of microorganisms with their animal host and are influenced by the living environment, diet and immune status of the host. gut microbes have been shown to be important for human disease and health, but little research exists in the gut microbiome of the amur tiger, which is one of the most endangered species in the world.201830509257
3' utrs in the eye of the tiger.many human genes generate mrnas with alternative 3' untranslated regions (3' utrs), which can modulate protein function. in a recent cell paper, ma and mayr (2018) unveil a major membraneless organelle, termed the tis granule-endoplasmic reticulum (tiger), which enables specific 3' utrs to encipher protein localization.201830513300
secreted cysteine-rich repeat proteins "screps": a novel multi-domain architecture.peptide toxins isolated from animal venom secretions have proven to be useful pharmacological tools for probing the structure and function of a number of molecular receptors. their molecular structures are stabilized by posttranslational formation of multiple disulfide bonds formed between sidechain thiols of cysteine residues, resulting in high thermal and chemical stability. many of these peptides have been found to be potent modulators of ion channels, making them particularly influential in ...201830524283
tn-core: a toolbox for integrating tn-seq gene essentiality data and constraint-based metabolic modeling.the design of synthetic cells requires a detailed understanding of the relevance of genes and gene networks underlying complex cellular phenotypes. transposon-sequencing (tn-seq) and constraint-based metabolic modeling can be used to probe the core genetic and metabolic networks underlying a biological process. integrating these highly complementary experimental and in silico approaches has the potential to yield a highly comprehensive understanding of the core networks of a cell. specifically, ...201930525460
functional characterization of a cdna encoding piwi protein in penaeus monodon and its potential roles in controlling transposon expression and spermatogenesis.piwi proteins comprise a subfamily of argonaute that plays a major role in germline development by association with a distinct class of small rnas called piwi interacting rna (pirna). although the functions of piwi in the development of germline cells as well as transposon regulation were reported in a number of mammalians and insects, developmental expression and function of piwi subfamily in crustaceans is poorly known. this study is aimed at cloning and characterization of a piwi cdna in the ...201930529395
natural concurrent infection of vibrio harveyi and v. alginolyticus in cultured hybrid groupers in malaysia.in september 2016, a marine fish farm operator in selangor, malaysia, reported a disease outbreak affecting juvenile hybrid groupers (camouflage grouper epinephelus polyphekadion × tiger grouper e. fuscoguttatus). the average daily mortality was 120 fish, resulting in a cumulative mortality rate of 29% within 10 d. the affected hybrid groupers displayed lethargy, excessive mucus production, rotten fins, congestion of livers and kidneys, and enlargement of spleens. microscopically, general conges ...201930536485
leydig cell tumor in an amur tiger (panthera tigris altaica).a 14-year and 8-month-old intact male amur tiger presented with an enlarged left testis, measuring 5.7 × 5.5 × 4.5 cm. the cut surface was mottled dark red to reddish brown in color. microscopically, the enlarged left testis comprised round or polygonal neoplastic cells arranged in a diffuse sheet pattern. these neoplastic cells had a hyperchromatic nucleus and an abundant eosinophilic cytoplasm. immunohistochemically, these neoplastic cells were positive for vimentin, chromogranin a, synaptophy ...201930541983
noninvasively diagnosing for fetal trisomy 21 by examining heterozygous single nucleotide polymorphisms in the placental specific genes on chromosome 21.noninvasive prenatal diagnosing for fetal trisomy 21 could help to avoid unnecessary amniocentesis. it is feasible to target the cell-free placental-specific mrna in maternal plasma, thus eliminating the interference from the maternal genetic background.201930544028
identification and in vitro evaluation of lipids from sclerotia of lignosus rhinocerotis for antioxidant and anti-neuroinflammatory activities.lignosus rhinocerotis (cooke) ryvarden (tiger milk mushroom) is traditionally used to treat inflammation triggered symptoms and illnesses such as cough, fever and asthma. the present study evaluated the in vitro antioxidant, cytotoxic and anti-neuroinflammatory activities of the extract and fractions of selerotia powder of l. rhinocerotis on brain microglial (bv2) cells. the ethyl acetate fraction had a total phenolic content of 0.30 ± 0.11 mg gae/g. this fraction had ferric reducing capacity of ...201630549604
noninvasive dna-based species and sex identification of asiatic wild dog (cuon alpinus).asiatic wild dog (cuon alpinus) or dhole is an endangered canid with fragmented distribution in south, east and southeast asia. the remaining populations of this species face severe conservation challenges from anthropogenic interventions, but only limited information is available at population and demography levels. here, we describe the novel molecular approaches for unambiguous species and sex identification from noninvasively collected dhole samples. we successfully tested these assays on 13 ...201830555094
caudal musculature in ground sharks, carcharhiniformes, with remarks on their phylogenetic interrelationships.relationships among families of the shark order carcharhiniformes are still being debated, for example, in relation to the inclusion of hammerhead sharks (sphyrnidae) and the tiger shark (galeocerdo cuvier) in the carcharhinidae. previous morphological studies have not analyzed the phylogenetic significance of axial caudal muscles among carcharhiniforms. the authors therefore describe here the axial muscles of the tail region (m. epaxialis, m. obliquus superioris, m. obliquus inferioris, m. flex ...201930556950
high-dose chemotherapy in relapsed or refractory metastatic germ-cell cancer: the scotland experience.to report outcomes from high-dose chemotherapy (hdct) and autologous stem-cell transplantation (asct) for metastatic germ-cell cancer in scotland.201930563754
decline of coastal apex shark populations over the past half century.overexploitation of large apex marine predators is widespread in the world's oceans, yet the timing and extent of declines are poorly understood. here we reconstruct a unique fisheries-independent dataset from a shark control programme spanning 1760 km of the australian coastline over the past 55 years. we report substantial declines (74-92%) of catch per unit effort of hammerhead (sphyrnidae), whaler (carcharhinidae), tiger shark (galeocerdo cuvier) and white sharks (carcharodon carcharias). fo ...201830564744
guidelines for patients with acute uncomplicated cystitis may not be a paper tiger: a call for its implementation in clinical routine. 201930564871
aedes albopictus (diptera: culicidae) on an invasive edge: abundance, spatial distribution, and habitat usage of larvae and pupae across urban and socioeconomic environmental gradients.the asian tiger mosquito [aedes albopictus (skuse)] is an invasive species of public health importance that is currently expanding its range in the northeast united states. effective ae. albopictus control depends on a thorough understanding of factors influencing their abundance, spatial distribution, and habitat preference. we conducted a series of container surveys for ae. albopictus larvae/pupae over 2 yr across nine sites in neighborhoods along its invasive range in southern new york. selec ...201930566612
comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk extender.comparative effect of slow and rapid freezing on sperm functional attributes and oxidative stress parameters of goat spermatozoa cryopreserved with tiger nut milk (tnm) extender was examined in this study. pooled semen samples obtained from west african dwarf (wad) goat bucks were diluted with tris-based extenders containing different levels of tnm (0, 5, 10, 15 and 20 ml/100 ml extender). the diluted semen samples were subjected to slow and rapid freezing for a period of 7 days and thereafter e ...201930570173
development and characterization of 33 microsatellite loci for the tiger frog hoplobatrachus rugulosus (wiegmann 1834) through transcriptome sequencing.the tiger frog hoplobatrachus rugulosus (wiegmann 1834) is a large robust dicroglossid frog widely distributed in southern china, malaysia, myanmar, vietnam and thailand. the escaped bred tiger frog introduced from thailand hybridized with chinese native population may have affected the genetic diversity of local chinese tiger frogs. however, previous microsatellite loci of this species do not offer enough information to construct the genetic map. here, we reported 33 new microsatellite loci fro ...201830574879
the nix locus on the male-specific homologue of chromosome 1 in aedes albopictus is a strong candidate for a male-determining factor.global concern over the rapid expansion of the asian tiger mosquito, aedes albopictus, and its vector competence has highlighted an urgent need to improve currently available population control methods, like the sterile insect technique. knowledge of the sex determination cascade is a prerequisite for the development of early-stage sexing systems. to this end, we have characterised the putative sex determination gene, nix, in this species. in aedes species the chromosome complement consists of t ...201830583734
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