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weird animals, sex, and genome evolution.making my career in australia exposed me to the tyranny of distance, but it gave me opportunities to study our unique native fauna. distantly related animal species present genetic variation that we can use to explore the most fundamental biological structures and processes. i have compared chromosomes and genomes of kangaroos and platypus, tiger snakes and emus, devils (tasmanian) and dragons (lizards). i particularly love the challenges posed by sex chromosomes, which, apart from determining s ...201829215911
the impacts of modern-use pesticides on shrimp aquaculture: an assessment for north eastern australia.the use of pyrethroid and neonicotinoid insecticides has increased in australia over the last decade, and as a consequence, increased concentrations of the neonicotinoid insecticide imidacloprid have been measured in australian rivers. previous studies have shown that non-target crustaceans, including commercially important species, can be extremely sensitive to these pesticides. most shrimp farms in australia are predominantly located adjacent to estuaries so they can obtain their required sali ...201829190596
the acute stresses role of the atypical 2-cys peroxiredoxin pmprx5 in black tiger shrimp (penaeus monodon) from biological immunity and environmental toxicity stress.as a unique atypical 2-cys peroxiredoxin (prx) of the prx-like superfamily, peroxiredoxin5 (prx5) possesses special properties, such as its enzymatic mechanism, wide subcellular distribution and high affinity for peroxides and peroxynitrite. prx5 plays a crucial role in oxidative stress, immune responses, cell apoptosis, proliferation, differentiation, intracellular signaling, the modulation of gene expression, ecdysis, etc. in this paper, we obtained a full-length prx5 cdna sequence (designated ...201829578049
physiochemical and functional properties of tiger puffer (takifugu rubripes) skin gelatin as affected by extraction conditions.the study investigated physiochemical and functional properties of gelatins from tiger puffer skin (gtps) extracted with warm-water method at 45, 55 and 65°c for 3, 6 and 12h. gtps45 exhibited strong γ-, β-, α1- and α2-chains, but they faded with the presence of fragments (<80kda) in other gtps. as the increase of temperature and time, amide i and a were shifted to higher wavenumber and their amplitudes in gtps65-12 decreased. lightness declined while redness and yellowness were enhanced. gel st ...201829155199
the response of glutathione peroxidase 1 and glutathione peroxidase 7 under different oxidative stresses in black tiger shrimp, penaeus monodon.glutathione peroxidase (gpx) is part of the enzymatic antioxidant system that can eliminate the peroxides produced as effect of reactions of molecules with reactive oxygen species (ros). we cloned two different gpx genes from penaeus monodon (black tiger shrimp). bioinformatics sequence analysis showed that pmgpx1 and pmgpx7 encoded polypeptides of 207 and 208 amino acids, respectively. pmgpx1 has a selenocysteine residue that is encoded by an opal codon 187tga189. the pmgpx1 and pmgpx7 expressi ...201829246783
unleashing the tiger - iatrogenic autoimmunity from cancer immunotherapy drugs.immune checkpoint inhibitors can lead to the development of organ and non-organ specific immune related adverse events.201829344404
community-based breastfeeding support with the tiger babies breastfeeding support tent.the tiger babies breastfeeding support tent was established by a baccalaureate nursing program in southeastern alabama to increase community awareness of the importance of breastfeeding and to provide a clean, convenient, and private location for nursing mothers during community events. local advertising was distributed before community events to promote awareness and support of the project. survey results indicate that 80% of mothers who used the tent's services would recommend the tent to thei ...201829628053
innovation strategy management survey of the chilean biomedical industry. assessment of windows of opportunities to reduce technological gaps.the convergence of different theories (ie, catch-up effect and windows of opportunities) allows for the interpretation of different "technological innovation gaps" in chile's biomedical industry. it is common knowledge that chile has always had an economy almost exclusively based on services, commodities, and mainly in the exploitation of natural resources with low value added. the literature confirms that countries that concentrate their economies on the knowledge, research, development, and co ...201829423976
food and feeding habits of gaur (bos gaurus) in highlands of central india: a case study at pench tiger reserve, madhya pradesh (india).indian gaur (bos gaurus) is one of nine species of wild oxen found in the world. they are largely confined to evergreen, semi-evergreen, and moist deciduous forests, but also occur in dry deciduous forest areas at the periphery of their range. according to the iucn red list ( 2017 ), the estimated population of gaur in india is between 15,000 and 35,000 individuals, and probably due to this, despite the gaur's vast range of distribution, they are listed as a vulnerable species by iucn and listed ...201829417898
freeze-dried spermatozoa: an alternative biobanking option for endangered species.in addition to the iconic wild species, such as the pandas and siberian tigers, an ever-increasing number of domestic species are also threatened with extinction. biobanking of spermatozoa could preserve genetic heritages of extinct species, and maintain biodiversity of existing species. because lyophilized spermatozoa retain fertilizing capacity, the aim was to assess whether freeze-dried spermatozoa are an alternative option to save endangered sheep breeds. to achieve this objective, semen was ...201829397252
crouching tiger, hidden dragon. 201829396928
looking deep into the eye-of-the-tiger in pantothenate kinase-associated neurodegeneration.a detailed delineation of the mr imaging changes in the globus pallidus in pantothenate kinase-associated neurodegeneration will be helpful for diagnosis and monitoring of patients. the aim of this study was to determine the morphologic spectrum of the "eye-of-the-tiger" sign and the topographic pattern of iron deposition in a group of patients with pantothenate kinase-associated neurodegeneration.201829371252
hematomas in tiger territory: an endovascular alternative to wading in.a 26-year-old man presented with gunshot wound to the epigastrium. at surgery, he was hemodynamically stable and had a tense hematoma with thrill in zone 2 (right side) and porta triad. after liver injury was controlled, he underwent percutaneous stenting of a renal artery-vena cava fistula and the hepatic artery injury was followed. historically, penetrating injury to zone 2 has mandated operative exploration. however, with the advent of endovascular options, in stable patients, catheter-based ...201829194100
microbiological stabilization of tiger nuts' milk beverage using ultra-high pressure homogenization. a preliminary study on microbial shelf-life extension.tiger nuts' milk beverages are highly perishable products. for this reason, the interest of food industry for their commercialization makes necessary the application of preservation treatments to prolong their shelf-life. in the current study, the effect of ultra-high pressure homogenization (uhph) on the microbiological and sensory qualities of tiger nuts' milk beverage was evaluated. characteristics of uhph-treated products (at 200 and 300 mpa, with inlet temperature of 40 °c) were compared wi ...201828941895
should we judge a tiger by its stripes? 201828940896
effects of acute temperature and salinity changes, body length and starvation on the critical swimming speed of juvenile tiger puffer, takifugu rubripes.the critical swimming speed (u crit, cm s-1) of juvenile tiger puffer takifugu rubripes was determined under different temperatures (15, 21, 25 and 30 °c), salinities (5, 10, 20, 32 and 40), body lengths (3.32, 4.08, 5.06 and 5.74 cm) and starvation days (1, 3, 6 and 9 days). acute temperature change, body length and starvation significantly influenced the u crit of tiger puffers, whereas acute salinity change had no significant effect. the u crit increased as the temperature increased from 15 t ...201829082461
improved egfr mutation detection using combined exosomal rna and circulating tumor dna in nsclc patient plasma.a major limitation of circulating tumor dna (ctdna) for somatic mutation detection has been the low level of ctdna found in a subset of cancer patients. we investigated whether using a combined isolation of exosomal rna (exorna) and cell-free dna (cfdna) could improve blood-based liquid biopsy for egfr mutation detection in non-small-cell lung cancer (nsclc) patients.201829216356
legal aspects of counteracting the trafficking of falsified medicines in the european union.the paper identifies key risks associated with the illegal production and sale of medicines. also there were generalized features of criminal responsibility for acts related to the trafficking of drugs in some member states of the eu and analyzed legal means of combating the falsified drugs today. the problem concerning falsification of medicines is particularly acute not only in developing countries but also in developed ones. fake is one in ten - twenty drug. the largest share of falsified dru ...201829064815
randomised comparison of judkins vs. tiger catheter in coronary angiography via the right radial artery: the judge study.the aim of this study was to compare the performance of the tiger-ii with judkins 3.5l/4r catheters in coronary angiography (cag) via the transradial approach (tra).201829061547
tissgdb: tissue-specific gene database in cancer.tissue-specific gene expression is critical in understanding biological processes, physiological conditions, and disease. the identification and appropriate use of tissue-specific genes (tissgenes) will provide important insights into disease mechanisms and organ-specific therapeutic targets. to better understand the tissue-specific features for each cancer type and to advance the discovery of clinically relevant genes or mutations, we built tissgdb (tissue specific gene database in cancer) avai ...201829036590
clinical effect of a mutation (p.glu322asp, c.966 g>t) in pank2 gene in a family with atypical pantothenate kinase-associated neurodegeneration.pantothenate-kinase-associated neurodegeneration (pkan) is a rare autosomal recessive disorder caused by mutations in the pantothenate kinase 2 (pank2) gene. many different mutations in the pank2 gene have been detected in association with pkan. a 20 year old female patient who had been suffering from progressive gait disorder for 1 year was found to have the 'eye-of-the-tiger sign' from the brain magnetic resonance imaging (mri). the same brain imaging findings were shown in the father and brot ...201830226968
untargeted metabolomics reveals links between tiger nut (cyperus esculentus l.) and its geographical origin by metabolome changes associated with membrane lipids.tiger nuts and tiger nut milk are well-known valencian products, and step-by-step these tubers and the tuber-based beverage are becoming more and more relevant products in international markets. however, the increasing demand and success of valencian tiger nuts did not allow protected designation of origin (pdo) tuber to supply the domestic and international markets. therefore, the verification of the geographical origin is highly required. in this research, the main objective was to combine an ...201829098962
high concentrations of perfluorooctane sulfonate in mucus of tiger puffer fish takifugu rubripes: a laboratory exposure study.distribution of perfluorooctane sulfonate (pfos) was investigated in tissues (plasma, blood clot, mucus, skin, liver, muscle, and gonad) of tiger puffer fish takifugu rubripes. a single dose of pfos was intraperitoneally injected at 0.1 mg/kg body weight with samples taken over a 14-day period. the highest concentration of pfos was found in the plasma, 861 ng/ml at 14 days, followed by the mucus, liver, blood clot, gonads, muscles, and skin of fish. a gradual upward trend in pfos concentration w ...201829098580
the tiger is extinct! (almost). 201828929606
polarized transilluminating dermoscopy: bedside trichoscopic diagnosis of trichothiodystrophy.trichothiodystrophy is a rare autosomal recessive disorder resulting in a broad range of systemic abnormalities. polarizing microscopy of the hair reveals the pathognomic "tiger tail" of alternating light and dark bands, but the need for a microscope prevents rapid bedside diagnosis. we describe a new technique for the bedside diagnosis of trichothiodystrophy using a handheld polarizing dermatoscope, precluding the need for microscopic examination.201828944975
the invasive asian tiger mosquito aedes albopictus (diptera: culicidae) in the czech republic: repetitive introduction events highlight the need for extended entomological surveillance.in the framework of a regional collaborative project between authorities and scientists, evidence was found of repeated introduction of aedes (stegomyia) albopictus (skuse) alongside the main road entrances (e461 and e65) connecting austria and the slovak republic with the czech republic. in comparison to data from 2012 (17 specimens collected on three occasions), the seasons 2016 (66 specimens on ten occasions) and 2017 (90 specimens on eight occasions) show an apparent increase of introduction ...201829856987
genetic characters of the globally spread tiger mosquito, aedes albopictus (diptera, culicidae): implications from mitochondrial gene coi.there has been a rapid global expansion of aedes albopictus, with varying biological characters and vector competence according to geographic and evolutionary origins of the invading populations. based on mitochondrial gene cytochrome c oxidase i (coi) sequences from both native and invasive regions, genetic characters of ae. albopictus were examined. phylogenetic analyses indicate lineage differentiation in the original areas, and only one of the lineages was involved in the worldwide expansion ...201829757513
chemical composition and assessment of larvicidal and repellent capacity of 14 lamiaceae essential oils against aedes albopictus.in the current laboratory study, 14 essential oils (eos) derived from 12 lamiaceae plant species and their major components were screened for their larvicidal and repellent properties against aedes albopictus, an invasive mosquito species of great medical importance. the results of toxicity bioassays revealed that the eos from thymus vulgaris, ocimum basilicum, origanum dictamnus, origanum majorana, and origanum vulgare, as well as their major components (terpenes), namely thymol, carvacrol, p-c ...201829717370
neighbors help neighbors control urban mosquitoes.the worldwide spread of invasive aedes mosquitoes and arboviral disease, have renewed the pressure for effective and sustainable urban mosquito control. we report on the success of a model we are confident will usher in a new era of urban mosquito control. the key innovation is the mobilization of neighbors guided by scientific advisors, an approach we termed citizen action through science (citizen acts). this approach was tested in a ne us town of approximately 1,000 residential yards infested ...201830361483
experimental risk assessment for chikungunya virus transmission based on vector competence, distribution and temperature suitability in europe, 2018.backgroundover the last decade, the abundant distribution of the asian tiger mosquito aedes albopictus in southern europe and the import of chikungunya virus (chikv) by infected travellers has resulted in at least five local outbreaks of chikungunya fever in france and italy. considering the ongoing spread of ae. albopictus to central europe, we performed an analysis of the europe-wide spatial risk of chikv transmission under different temperature conditions. methods:ae. albopictus specimens fro ...201830043726
diapause-associated changes in the lipid and metabolite profiles of the asian tiger mosquito, aedes albopictus.diapause is an alternative life-history strategy that allows organisms to enter developmental arrest in anticipation of unfavorable conditions. diapause is widespread among insects and plays a key role in enhancing overwinter survival as well as defining the seasonal and geographic distributions of populations. next-generation sequencing has greatly advanced our understanding of the transcriptional basis for this crucial adaptation but less is known about the regulation of embryonic diapause phy ...201830385483
first record of aedes albopictus (diptera: culicidae) and second record of aedes japonicus (diptera: culicidae) parasitized by water mites (acari: hydrachnidiae) in north america.the primary aim of this study was to determine if the asian tiger mosquito (aedes albopictus (skuse)) (diptera: culicidae), that vectors a number of arboviruses, is parasitized by water mites (acari: prostigmata: parasitengonina: hydrachnidiae). larval water mite parasitism of adult mosquitoes has been shown to reduce mosquito fecundity and life span in the laboratory, and impact some populations in the field. water mites have been considered for biocontrol purposes and used to age-grade mosquit ...201830020480
nighttime applications of two formulations of pyrethroids are effective against diurnal aedes albopictus.the successful control of aedes albopictus requires a multifaceted approach using a variety of integrated pest management techniques. because this species is diurnal, nighttime ultra-low volume adulticide applications seem likely to miss resting mosquitoes and, therefore, are often met with skepticism. the goal of this study was to compare the efficacy of nighttime applications of pyrethroids with and without prallethrin to control caged and field populations of ae. albopictus. during august and ...201831442153
land use and larval habitat increase aedes albopictus (diptera: culicidae) and culex quinquefasciatus (diptera: culicidae) abundance in lowland hawaii.vector abundance plays a key role in transmission of mosquito-borne disease. in hawaii, aedes albopictus (skuse) (diptera: culicidae), the asian tiger mosquito, has been implicated in locally-transmitted dengue outbreaks, while culex quinquefasciatus say (diptera: culicidae), the southern house mosquito, is the primary vector of avian malaria, a wildlife disease that has contributed to declines and extinctions of native hawaiian birds. despite the importance of these introduced species to human ...201830085189
genetic and environmental influences on the size-fecundity relationship in aedes albopictus (diptera: culicidae): impacts on population growth estimates?population growth models are integral to ecological studies by providing estimates of population performance across space and time. several models have been developed that estimate population growth through correlates of demographic traits, as measuring each parameter of the model can be prohibitive in experimental studies. since differences in female size can accurately reflect changes in fecundity for many taxa, livdahl and sugihara developed a population growth index that incorporates size-fe ...201830071049
cannibalism in temporary waters: simulations and laboratory experiments revealed the role of spatial shape in the mosquito aedes albopictus.cannibalism is a commonly observed phenomenon in arthropod species having relevant consequences for population dynamics and individual fitness. it is a context-dependent behaviour and an understanding of the factors affecting cannibalism rate is crucial to highlight its ecological relevance. in mosquitoes, cannibalism between larval stages has been widely documented, and the role of density, food availability and length of contact between individuals also ascertained. however, although mosquitoe ...201829813108
water associated with residential areas and tourist resorts is the key predictor of asian tiger mosquito presence on a mediterranean island.the asian tiger mosquito, aedes albopictus (diptera: culicidae), is a highly invasive species and a vector of several viruses of serious concern to public health. investigating the habitat selection of this species at small to medium scales is essential to the planning of effective prevention and control campaigns. the present group considered detailed data for this species' presence/absence collected at 228 sites on mallorca island (spain) in autumn 2015, 3 years after the first detection of th ...201829969158
first report on the application of near-infrared spectroscopy to predict the age of aedes albopictus skuse.to date, no methodology has been described for predicting the age of aedes albopictus skuse mosquitoes, commonly known as asian tiger mosquitoes. in this study, we report the potential of near-infrared spectroscopy (nirs) technique for characterizing the age of female laboratory reared ae. albopictus. using leave-one-out cross-validation analysis on a training set, laboratory reared mosquitoes preserved in rnalater for up to a month were assessed at 1, 3, 7, 9, 13, 16, 20 and 25 days post emerge ...201829941924
alien species and human health: austrian stakeholder perspective on challenges and solutions.no saturation in the introduction, acceleration of spread and the increasing impacts of alien species are a characteristic feature of the anthropocene. concomitantly, alien species affecting human health are supposed to increase, mainly due to increasing global trade and climate change. in this study, we assess challenges and solutions posed by such species to the public health sector in austria over the next few decades. we did so using an online questionnaire circulated to 131 experts and stak ...201830424500
construction of a genetic sexing strain for aedes albopictus: a promising tool for the development of sterilizing insect control strategies targeting the tiger mosquito.aedes albopictus is an invasive mosquito species of global medical concern as its distribution has recently expanded to africa, the americas and europe. in the absence of prophylaxis protecting human populations from emerging arboviruses transmitted by this mosquito species, the most straightforward control measures rely on the suppression or manipulation of vector natural populations. a number of environmental-friendly methods using mass releases of sterilizing males are currently under develop ...201830583741
effectiveness of a field trap barrier system for controlling aedes albopictus: a "removal trapping" strategy.aedes aegypti and aedes albopictus are the main vectors for the transmission of several viral pathogens, in particular, dengue, zika and chikungunya. in the absence of vaccines and treatment, control of aedes mosquitoes is the only means of keeping these diseases in check. aedes control is difficult, and it is, therefore, necessary to evaluate the efficacy of novel control methods, particularly those targeting adult and exophilic ae. albopictus populations.201829463300
the nix locus on the male-specific homologue of chromosome 1 in aedes albopictus is a strong candidate for a male-determining factor.global concern over the rapid expansion of the asian tiger mosquito, aedes albopictus, and its vector competence has highlighted an urgent need to improve currently available population control methods, like the sterile insect technique. knowledge of the sex determination cascade is a prerequisite for the development of early-stage sexing systems. to this end, we have characterised the putative sex determination gene, nix, in this species. in aedes species the chromosome complement consists of t ...201830583734
a scoping review of published literature on chikungunya virus.chikungunya virus (chikv) has caused several major epidemics globally over the last two decades and is quickly expanding into new areas. although this mosquito-borne disease is self-limiting and is not associated with high mortality, it can lead to severe, chronic and disabling arthritis, thereby posing a heavy burden to healthcare systems. the two main vectors for chikv are aedes aegypti and aedes albopictus (asian tiger mosquito); however, many other mosquito species have been described as com ...201830496207
plant-derived compounds regulate formation of the insect juvenile hormone receptor complex.insect growth regulators (igrs) are attractive pest control agents due to their high target specificity and relative safety to the environment. recently, plants have been shown to synthesize igrs that affect the insect juvenile hormone (jh) as a part of their defense mechanisms. using a yeast two-hybrid system transformed with the aedes aegypti jh receptor as a reporter system, we identified several jh agonists (jhas) and antagonists (jhans) causing retardation in the ovarian development of fema ...201830195384
rapid evolution and the genomic consequences of selection against interspecific mating.while few species introduced into a new environment become invasive, those that do provide critical information on ecological mechanisms that determine invasions success and the evolutionary responses that follow invasion. aedes albopictus (the asian tiger mosquito) was introduced into the naturalized range of aedes aegypti (the yellow fever mosquito) in the united states in the mid-1980s, resulting in the displacement of a. aegypti in much of the south-eastern united states. the rapid displacem ...201830069966
immunostimulation and yellow head virus (yhv) disease resistance induced by a lignin-based pulping by-product in black tiger shrimp (penaeus monodon linn.).yellow head virus (yhv) is classified as one of the most serious pathogens causing a harmful disease in many penaeids, especially black tiger shrimp (penaeus monodon), with high economic loss. to determine a potent and practical prophylactic strategy for controlling this disease, the toxicity of the by-product kraft lignin and its ability to control severe yhv infection were investigated in juvenile black tiger shrimp (15.9 ± 1.2 g body weight). the median lethal dosage at 96 h (96-hrs ld50) of ...201829162544
dispersion and oviposition of aedes albopictus in a brazilian slum: initial evidence of asian tiger mosquito domiciliation in urban environments.aedes albopictus, originally considered as a secondary vector for arbovirus transmission, especially in areas where this species co-exist with aedes aegypti, has been described in most regions of the world. dispersion and domiciliation of ae. albopictus in a complex of densely urbanized slums in rio de janeiro, southeastern brazil, was evidenced. in this study, we tested the hypotheses that 1) ae. albopictus distribution in urban slums is negatively related to distance from vegetation, and 2) th ...201829684029
modulation of cortisol responses to an acute stressor in zebrafish visually exposed to heterospecific fish during development.studies regarding predator-prey relationships have often focused on prey assessment and the responses to predation risk, but few have verified the relationship in the presence or absence of visual contact with a predator (e.g., tiger oscar, astronotus ocellatus) or a nonpredator (e.g., goldfish, carassius auratus) during the developmental phase, which could alter several physiological and neuroendocrine mechanisms in adulthood. herein, we determined responses to physical (chasing with a net) and ...201829369748
inhibition of protein glycation by tiger milk mushroom [lignosus rhinocerus(cooke) ryvarden] and search for potential anti-diabetic activity-related metabolic pathways by genomic and transcriptomic data mining.naturally occurring anti-glycation compounds have drawn much interest in recent years as they show potential in reducing or preventing the risk of chronic complications for diabetic patients. in this study, annotation of the genome-transcriptome data from tiger milk mushroom (lignosus rhinocerus, syn.lignosus rhinocerotis) to plantcyc enzymes database identified transcripts that are related to anti-diabetic properties, and these include genes that are involved in carotenoid and abscisic acid bio ...201829491836
habitat geography around hawaii's oceanic islands influences tiger shark (galeocerdo cuvier) spatial behaviour and shark bite risk at ocean recreation sites.we compared tiger shark (galeocerdo cuvier) spatial behaviour among 4 hawaiian islands to evaluate whether local patterns of movement could explain higher numbers of shark bites seen around maui than other islands. our sample consisted of 96 electronically-tagged (satellite and acoustic transmitters) tiger sharks, individually tracked for up to 6 years. most individuals showed fidelity to a specific 'home' island, but also swam between islands and sometimes ranged far (up to 1,400 km) offshore. ...201829563552
sargassum wightii-synthesized zno nanoparticles - from antibacterial and insecticidal activity to immunostimulatory effects on the green tiger shrimp penaeus semisulcatus.the green synthesis of metal nanoparticles using phytochemical from marine seaweeds is a fast-growing research field in nanotechnology. here, the biosynthesis of zinc oxide nanoparticles was achieved using the hot water extract of sargassum wightii. the hot water extract prepared from s. wightii (h sw) and zno nps were studied by uv-visible and ftir spectroscopy, sem and xrd. then, both products were evaluated for antibiofilm activity towards aquatic pathogens. the nanoparticles' immunostimulati ...201829754050
wssv-responsive gene expression under the influence of pmvrp15 suppression.the viral responsive protein 15 from black tiger shrimp penaeus monodon (pmvrp15), is highly up-regulated and produced in the hemocytes of shrimp with white spot syndrome virus (wssv) infection. to investigate the differential expression of genes from p. monodon hemocytes that are involved in wssv infection under the influence of pmvrp15 expression, suppression subtractive hybridization (ssh) of pmvrp15-silenced shrimp infected with wssv was performed. the 189 cdna clones of the forward library ...201829017938
toll-receptor 9 gene in the black tiger shrimp (penaeus monodon) induced the activation of the tlr-nf-κb signaling pathway.toll receptors are important pathogen recognition receptors (prrs) in shrimps, which play a vital role in defending against virus and bacterial challenge. in this paper, the characterization and functional analysis of a toll9 receptor gene from penaeus monodon was performed in hek293t cells. data showed that pmtoll9 can activate the nf-κb promoter activities of tlr pathway, while isre and ifn-β promoter cannot be activated obviously in hek293t cells using dual-luciferase reporter system. the dow ...201828982619
the asian tiger mosquito aedes albopictus (diptera: culicidae) in central germany: surveillance in its northernmost distribution area.the invasive asian tiger mosquito aedes albopictus has recently been observed in southern germany for the first time to reproduce and even overwinter north of the alps. after the accidental capture of adult specimens in jena, german federal state of thuringia, in mid-2015, regular inspections brought forth developmental stages until autumn 2015, indicating local reproduction. surveillance activities implemented in 2016 showed larvae already in early may, suggesting overwintering, and throughout ...201830145257
life histories and other biological characteristics enabling the establishment of aedes albopictus in the san gabriel valley, california.since aedes albopictus was discovered in 2011 in the san gabriel valley it has become widespread despite the "harsh" environmental conditions and intense efforts to control or eliminate it. species introduced into a new area may survive, thrive, or disappear depending on whether its new environment is suitable. the san gabriel valley mosquito and vector control district expended considerable resources from 2011 to 2015 to eradicate this invasive species or, at a minimum, control and manage its s ...201831442161
genetic analysis of aedes albopictus (diptera, culicidae) reveals a deep divergence in the original regions.aedes albopictus has been described as one of the 100 worst invasive species in the world. this mosquito originated from southeastern asia and currently has a widespread presence in every continent except antarctica. the rapid global expansion of ae. albopictus has increased public health concerns about arbovirus-related disease threats. adaptation, adaption to novel areas is a biological challenge for invasive species, and the underlying processes can be studied at the molecular level. in this ...201829729282
pirna profiling of dengue virus type 2-infected asian tiger mosquito and midgut tissues.the asian tiger mosquito, aedes albopictus, is a competent vector for the majority of arboviruses. the mosquito innate immune response is a primary determinant for arthropod-borne virus transmission, and the midgut is the first barrier to pathogen transmission. mosquito antiviral immunity is primarily mediated by the small interfering rna pathway. however, the roles that the p-element induced wimpy testis (piwi)-interacting rna (pirna) pathway play in antiviral immunity in ae. albopictus and its ...201829690553
detection of the invasive mosquito species aedes (stegomyia) albopictus (diptera: culicidae) in portugal.the asian tiger mosquito aedes albopictus is an invasive mosquito originating from the asia-pacific region. this species is of major concern to public and veterinary health because of its vector role in the transmission of several pathogens, such as chikungunya, dengue, and zika viruses. in portugal, a national vector surveillance network (rede de vigil&acirc;ncia de vectores&mdash;revive) is responsible for the surveillance of autochthonous, but also invasive, mosquito species at points of entr ...201829690531
the asian tiger mosquito, aedes (stegomyia) albopictus (skuse), a vector of dengue, chikungunya and zika viruses, reaches portugal (diptera: culicidae).the asian tiger mosquito, aedes (stegomyia) albopictus (skuse), is a tropical species originally from south-eastern asia (hawley, 1988). it has experienced a rampant human-mediated range expansion since the 1970s to now occupy almost the whole of the tropical and subtropical areas of the world. as an eclectic haematophagous species, it attacks humans and is able to use a number of man-made and natural structures where stagnant water is present (estrada-franco craig, 1995). eggs are able to survi ...201829690129
shared larval rearing environment, sex, female size and genetic diversity shape ae. albopictus bacterial microbiota.the asian tiger mosquito aedes albopictus became of public health concern as it can replicate and transmit viral and filarial pathogens with a strong invasive success over the world. various strategies have been proposed to reduce mosquito population's vectorial capacity. among them, symbiotic control of mosquito borne disease offers promising perspectives. such method is likely to be affected by the dynamics of mosquito-associated symbiotic communities, which might in turn be affected by host g ...201829641577
investigation of temperature conditions in swiss urban and suburban microhabitats for the overwintering suitability of diapausing aedes albopictus eggs.in switzerland, the invasive asian tiger mosquito, aedes albopictus, is firmly established in the canton of ticino, south of the alps. according to a large-scale distribution model developed in 2013, suitable climatic conditions for the establishment of ae. albopictus north of the alps are found in basel and geneva while zurich appears to be characterized by winters currently being too cold for survival of diapausing eggs. however, the spatial resolution of large-scale distribution models might ...201829587850
local extinction of the asian tiger mosquito (aedes albopictus) following rat eradication on palmyra atoll.the asian tiger mosquito, aedes albopictus, appears to have been extirpated from palmyra atoll following rat eradication. anecdotal biting reports, collection records, and regular captures in black-light traps showed the species was present before rat eradication. since then, there have been no biting reports and no captures over 2 years of extensive trapping (black-light and scent traps). by contrast, the southern house mosquito, culex quinquefasciatus, was abundant before and after rat eradica ...201829491026
forecasting the spatial and seasonal dynamic of aedes albopictus oviposition activity in albania and balkan countries.the increasing spread of the asian tiger mosquito, aedes albopictus, in europe and us raises public health concern due to the species competence to transmit several exotic human arboviruses, among which dengue, chikungunya and zika, and urges the development of suitable modeling approach to forecast the spatial and temporal distribution of the mosquito. here we developed a dynamical species distribution modeling approach forecasting ae. albopictus eggs abundance at high spatial (0.01 degree wgs8 ...201829432489
areas with high hazard potential for autochthonous transmission of aedes albopictus-associated arboviruses in germany.the intensity and extent of transmission of arboviruses such as dengue, chikungunya, and zika virus have increased markedly over the last decades. autochthonous transmission of dengue and chikungunya by aedes albopictus has been recorded in southern europe where the invasive mosquito was already established and viraemic travelers had imported the virus. ae. albopictus populations are spreading northward into germany. here, we model the current and future climatically suitable regions for ae. alb ...201829914102
larval superiority of culex pipiens to aedes albopictus in a replacement series experiment: prospects for coexistence in germany.the asian tiger mosquito aedes albopictus is an extremely invasive, globally distributed and medically important vector of various human and veterinary pathogens. in germany, where this species was recently introduced, its establishment may become modulated by interspecific competition from autochthonous mosquito species, especially culex pipiens (s.l.). while competitive superiority of ae. albopictus to cx. pipiens (s.l.) has been described elsewhere, it has not been assessed in the epidemiolog ...201829394910
new dates on dingo bones from madura cave provide oldest firm evidence for arrival of the species in australia.the dingo is the only placental land mammal aside from murids and bats to have made the water crossings to reach australia prior to european arrival. it is thought that they arrived as a commensal animal with people, some time in the mid holocene. however, the timing of their arrival is still a subject of major debate with published age estimates varying widely. this is largely because the age estimates for dingo arrival are based on archaeological deposit dates and genetic divergence estimates, ...201830026564
letting the 'cat' out of the bag: pouch young development of the extinct tasmanian tiger revealed by x-ray computed tomography.the tasmanian tiger or thylacine (thylacinus cynocephalus) was an iconic australian marsupial predator that was hunted to extinction in the early 1900s. despite sharing striking similarities with canids, they failed to evolve many of the specialized anatomical features that characterize carnivorous placental mammals. these evolutionary limitations are thought to arise from functional constraints associated with the marsupial mode of reproduction, in which otherwise highly altricial young use the ...201829515893
effects of human impacts on habitat use, activity patterns and ecological relationships among medium and small felids of the atlantic forest.competition theory and niche theory suggest that two morphologically similar species may coexist by reducing the overlap of at least one dimension of their ecological niche. the medium and small neotropical felids are an interesting group of carnivore species for studying intraguild competition. due to differences in size it is expected that the larger ocelot exert strong interference competition on the smaller felids (southern tiger cat, margay and jaguarundi); which, in turn, may exert exploit ...201830067785
a note on wildlife poisoning cases from kerala, south india.wildlife poisoning is an important conservation threat for endangered species in india. there are no publications in the scientific literature that identify the specific poisons or chemicals involved in wildlife poisoning cases from the state of kerala. in this report, all cases of wildlife mortality recorded between 2011 and 2013 at the office of the assistant forest veterinary officer, periyar tiger reserve in kerala were reviewed and cases where poisoning was considered as a differential diag ...201830820194
relationships between humans and ungulate prey shape amur tiger occurrence in a core protected area along the sino-russian border.large carnivore populations are globally threatened by human impacts. better protection could benefit carnivores, co-occurring species, and the ecosystems they inhabit. the relationship between carnivores and humans, however, is not always consistent in areas of high human activities and is often mediated through the effects of humans on their ungulate prey. to test assumptions regarding how prey abundance and humans affect carnivore occurrence, density, and daily activity patterns, we assessed ...201830598766
mangifera indica l. leaf extract in combination with luteolin or quercetin enhances vo2peak and peak power output, and preserves skeletal muscle function during ischemia-reperfusion in humans.it remains unknown whether polyphenols such as luteolin (lut), mangiferin and quercetin (q) have ergogenic effects during repeated all-out prolonged sprints. here we tested the effect of mangifera indica l. leaf extract (mle) rich in mangiferin (zynamite®) administered with either quercetin (q) and tiger nut extract (tne), or with luteolin (lut) on sprint performance and recovery from ischemia-reperfusion. thirty young volunteers were randomly assigned to three treatments 48 h before exercise. t ...201829937737
genomic structure, expression, and functional characterization of checkpoint kinase 1 from penaeus monodon.chk1 is a cell-cycle regulator. chk1 has been identified in organisms ranging from yeast to humans, but few researchers have studied chk1 in shrimps. we cloned chk1 from the black tiger shrimp (penaeus monodon). the full-length cdna sequence of pmchk1 had 3,334 base pairs (bp), with an open reading frame of 1,455 bp. the complete genomic sequence of pmchk1 (11,081 bp) contained 10 exons separated by nine introns. qrt-pcr showed that pmchk1 was highly expressed in the ovaries and gills of p. mono ...201829795680
microplastics in different tissues of fish and prawn from the musa estuary, persian gulf.commercially-important species of fish and a crustacean from four sites in the musa estuary and a site in the persian gulf have been analysed for the presence and location of microplastics (mps). a total of 828 mps were detected in the guts (gastrointestinal tracts), skin, muscle, gills and liver of demersal and pelagic fish (platycephalus indicus, saurida tumbil, sillago sihama, cynoglossus abbreviatus) from all five sites and in the exoskeleton and muscle of the tiger prawn, penaeus semisulcat ...201829684694
characterizing conflict between humans and big cats panthera spp: a systematic review of research trends and management opportunities.conservation of big cats (panthera spp.), a taxonomic group including tigers, lions, jaguars, leopards and snow leopards, is a daunting challenge. as expanding human populations across panthera range countries exacerbate competition for land and prey, conflicts between humans and big cats are inevitable. through a systematic review of the peer-reviewed literature published from 1991 to 2014 and indexed in web of science and google scholar (186 articles), our study explored the current state of k ...201830226894
spatio-temporal patterns of attacks on human and economic losses from wildlife in chitwan national park, nepal.wildlife attacks on humans and economic losses often result in reduced support of local communities for wildlife conservation. information on spatial and temporal patterns of such losses in the highly affected areas contribute in designing and implementing effective mitigation measures. we analyzed the loss of humans, livestock and property caused by wildlife during 1998 to 2016, using victim family's reports to chitwan national park authorities and buffer zone user committees. a total of 4,014 ...201829672538
practical application of laparoscopic oviductal artificial insemination for the propagation of domestic cats and wild felids.ai was first reported in cats almost 50 years ago but, unlike ai in other domesticated animals (e.g. dogs, cattle, horses), has not been widely used for routine propagation by veterinarians or breeders. anatomical and physiological challenges with cats have hindered the efficiency of ai using standardised transcervical approaches applied to other species. development of laparoscopic oviductal ai (lo-ai) has helped overcome some of these barriers and, during the past 7 years, produced high pregna ...201832188540
papillary architecture of the lingual surface in the puma (puma concolor).this research presents the first anatomical description of the tongue and lingual papillae of the mountain lion (puma). the tongues of three adult male pumas were used in this study. the tongues were dissected and studied firstly by gross and stereomicroscopy. samples of each part were processed by study with scanning electron microscopy. the margins of the lingual apex were surrounded by numerous filiform papillae, which had a bulky papillary body and a bifurcated tip. on the dorsal surface of ...201829152772
characterization and expression analysis of a chitinase gene (pmchi-5) from black tiger shrimp (penaeus monodon) under pathogens infection and ambient ammonia-n stress.chitinases are crucial enzymes for crustaceans. previous researches had already revealed that chitinases play important roles in digestion, molting and defense against viruses. in the present study, a chitinase cdna was identified from black tiger shrimp (penaeus monodon) and designated as pmchi-5. the full-length pmchi-5 cdna was 2860 bp in size, containing an open reading frame (orf) of 1731 bp that encoded a protein of 576 amino acids with a deduced molecular weight of 64.8 kda. expression of ...201829100985
transcriptome analysis provides insights into the immune responsive pathways and genes in the head kidney of tiger grouper (epinephelus fuscoguttatus) fed with spatholobus suberectus, phellodendron amurense, or eclipta prostrata.the tiger grouper, epinephelus fuscoguttatus, is an economically important fish in southeast asia but has been plagued by several diseases. spatholobus suberectus (s), phellodendron amurense (p), and eclipta prostrate (e) are three commonly used chinese medicinal herbs. although previous pharmacological and clinical studies indicated that s, p, and e possess a variety of beneficial functions in mammals, little is known about their functions in farmed fish and the underlying molecular mechanism o ...201829222026
correction: dengue virus serotype 2 infection alters midgut and carcass gene expression in the asian tiger mosquito, aedes albopictus.[this corrects the article doi: 10.1371/journal.pone.0171345.].201829370309
a cytosolic sensor, pmddx41, mediates antiviral immune response in black tiger shrimp penaeus monodon.dead (asp-glu-ala-asp)-box polypeptide 41 (ddx41), a receptor belonging to the dexd family, has recently been identified as an intracellular dna sensor in vertebrates. here, we report on the identification and functional characterization of pmddx41, the first cytosolic dna sensor in shrimp. by searching a penaeus monodon expressed sequence tag (est) database (http://pmonodon.biotec.or.th), three cdna fragments exhibiting similarity to ddx41 in various species were identified and assembled, resul ...201829248385
genome of the tasmanian tiger provides insights into the evolution and demography of an extinct marsupial carnivore.the tasmanian tiger or thylacine (thylacinus cynocephalus) was the largest carnivorous australian marsupial to survive into the modern era. despite last sharing a common ancestor with the eutherian canids ~160 million years ago, their phenotypic resemblance is considered the most striking example of convergent evolution in mammals. the last known thylacine died in captivity in 1936 and many aspects of the evolutionary history of this unique marsupial apex predator remain unknown. here we have se ...201829230027
aedes albopictus (skuse, 1895) (diptera: culicidae) in greece: 13 years of living with the asian tiger mosquito.aedes (stegomyia) albopictus (skuse, 1895) (diptera: culicidae), commonly known as the asian tiger mosquito, is an invasive mosquito species of public health significance, well established in many countries worldwide. in greece, it was first recorded in corfu and thesprotia between 2003 and 2004. in the following years, further distribution and establishment of ae. albopictus in greece have been confirmed in many regional units of the country. in the current study, we report the invasion history ...201829275504
characterizing environmental suitability of aedes albopictus (diptera: culicidae) in mexico based on regional and global niche models.the asian tiger mosquito, aedes albopictus (skuse) (diptera: culicidae), is an invasive species and a vector of numerous human pathogens, including chikungunya, dengue, yellow fever, and zika viruses. this mosquito had been reported from 36 geographic locations in mexico by 2005, increasing to 101 locations by 2010 and 501 locations (spanning 16 states) by 2016. here we modeled the occupied niche for ae. albopictus in mexico to characterize the environmental conditions related to its presence, a ...201829186544
vector-borne diseases and climate change: a european perspective.climate change has already impacted the transmission of a wide range of vector-borne diseases in europe, and it will continue to do so in the coming decades. climate change has been implicated in the observed shift of ticks to elevated altitudes and latitudes, notably including the ixodes ricinus tick species that is a vector for lyme borreliosis and tick-borne encephalitis. climate change is also thought to have been a factor in the expansion of other important disease vectors in europe: aedes ...201829149298
first report of aedes (stegomyia) albopictus (skuse) (diptera: culicidae), the asian tiger mosquito, in ecuador.aedes (stegomyia) albopictus (skuse), (diptera: culicidae), the asian tiger mosquito, is one of the most widespread invasive vector-borne disease insect in tropical and temperate zones. this species has invaded the americas over the past 3 decades and has spread to six countries. we report ae. albopictus in guayaquil city, the first time it has been identified in ecuador. outdoor bg-sentinel traps without lures collected a total of 21 ae. albopictus.201829029173
a comparative modeling study on non-climatic and climatic risk assessment on asian tiger mosquito (aedes albopictus).aedes albopictus, the asian tiger mosquito, vector of chikungunya, dengue fever and zika viruses, has proven its hardy adaptability in expansion from its natural asian, forest edge, tree hole habitat on the back of international trade transportation, re-establishing in temperate urban surrounds, in a range of water receptacles and semi-enclosures of organic matter. conventional aerial spray mosquito vector controls focus on wetland and stagnant water expanses, proven to miss the protected hollow ...201829576954
antimicrobial activity of a serine proteinase inhibitor spipm5 from the black tiger shrimp penaeus monodon.a two-domain kazal-type serine proteinase inhibitor, spipm5, from penaeus monodon was studied. its transcript was expressed in all tissues tested including the hemocytes, stomach, gill, lymphoid organ, muscle, intestine and heart albeit less in hepatopancreas and eyestalk. the expression of spipm5 gene was also up-regulated by heat stress, white spot syndrome virus (wssv) infection and yellow head virus (yhv) infection. injection of recombinant rspipm5 protein into normal shrimp to mimic heat st ...201829601993
combining abundance and performance data reveals how temperature regulates coastal occurrences and activity of a roaming apex predator.the redistribution of species has emerged as one of the most pervasive impacts of anthropogenic climate warming, and presents many societal challenges. understanding how temperature regulates species distributions is particularly important for mobile marine fauna such as sharks given their seemingly rapid responses to warming, and the socio-political implications of human encounters with some dangerous species. the predictability of species distributions can potentially be improved by accounting ...201829516588
lack of multiple paternity in the oceanodromous tiger shark (galeocerdo cuvier).multiple paternity has been documented as a reproductive strategy in both viviparous and ovoviviparous elasmobranchs, leading to the assumption that multiple mating may be ubiquitous in these fishes. however, with the majority of studies conducted on coastal and nearshore elasmobranchs that often form mating aggregations, parallel studies on pelagic, semi-solitary species are lacking. the tiger shark (galeocerdo cuvier) is a large pelagic shark that has an aplacental viviparous reproductive mode ...201829410842
tiger sharks can connect equatorial habitats and fisheries across the atlantic ocean basin.increasing our knowledge about the spatial ecology of apex predators and their interactions with diverse habitats and fisheries is necessary for understanding the trophic mechanisms that underlie several aspects of marine ecosystem dynamics and for guiding informed management policies. a preliminary assessment of tiger shark (galeocerdo cuvier) population structure off the oceanic insular system of fernando de noronha (fen) and the large-scale movements performed by this species in the equatoria ...201728926627
systemic amyloidosis in an african tiger snake (telescopus semiannulatus).an adult male african tiger snake (telescopus semiannulatus) was diagnosed with disseminated mycobacteriosis and a hepatic biliary cystadenocarcinoma. histologically, the spleen was largely replaced by extracellular deposits of eosinophilic, fibrillar to hyaline material. similar material was also present in the testicular interstitium and occasional blood vessel walls. this material was congophilic with strong green birefringence under polarized light and emitted fluorescence when bound to the ...201728942295
systematic review: land cover, meteorological, and socioeconomic determinants of aedes mosquito habitat for risk mapping.asian tiger and yellow fever mosquitoes (aedes albopictus and ae. aegypti) are global nuisances and are competent vectors for viruses such as chikungunya (chikv), dengue (dv), and zika (zikv). this review aims to analyze available spatiotemporal distribution models of aedes mosquitoes and their influential factors. a combination of five sets of 3-5 keywords were used to retrieve all relevant published models. five electronic search databases were used: pubmed, medline, embase, scopus, and google ...201729035317
decoupling of active and passive reasons for the invasion dynamics of aedes albopictus skuse (diptera: culicidae): comparisons of dispersal history in the apennine and florida peninsulas.aedes albopictus is an important vector of several diseases including dengue- and chikungunya fever and is a potential vector of zika-fever. the invasion dynamics of aedes albopictus was reconsidered by comparing the temperature-related development of the mosquito with the observed real geographical distribution in florida and in italy. the potential number of generations and the annual dispersal distances of the mosquito were calculated for the estimates. the estimated total dispersals are 3.6- ...201729125256
forest canopy, water level, and biopesticide interact to determine oviposition habitat selection in aedes albopictus.understanding how interacting abiotic and biotic factors influence colonization rates into different habitat types is critical for both conserving and controlling species. for example, the rapid global spread of asian tiger mosquitoes, aedes albopictus, has reduced native species abundances and produced disease outbreaks. fortunately, bacterial endospores of two bacillus species (biospesticide) are highly lethal to ae. albopictus larvae and have been commercially developed to reduce populations. ...201729125247
estimating the extinction date of the thylacine with mixed certainty data.the thylacine (thylacinus cynocephalus), one of australia's most characteristic megafauna, was the largest marsupial carnivore until hunting, and potentially disease, drove it to extinction in 1936. although thylacines were restricted to tasmania for 2 millennia prior to their extinction, recent so-called plausible sightings on the cape york peninsula in northern queensland have emerged, leading some to speculate the species may have persisted undetected. we compiled a data set that included phy ...201729067718
deciphering the olfactory repertoire of the tiger mosquito aedes albopictus.the asian tiger mosquito aedes albopictus is a highly invasive species and competent vector of several arboviruses (e.g. dengue, chikungunya, zika) and parasites (e.g. dirofilaria) of public health importance. compared to other mosquito species, ae. albopictus females exhibit a generalist host seeking as well as a very aggressive biting behaviour that are responsible for its high degree of nuisance. several complex mosquito behaviours such as host seeking, feeding, mating or oviposition rely on ...201729020917
a multiplex bead-based assay for immune gene expression analysis in shrimp.here, we developed a 9-plex bead-based array as a tool to evaluate molecular effects on transcription levels of immune-related genes in the black tiger shrimp (penaeus monodon). the bead array technology allows simultaneous detection of multiple target genes in a single sample, reducing time, labor and cost. the oligonucleotide probes were designed to target eight immune-related genes that involve in antimicrobial activity, melanization, pathogen pattern recognition proteins, lysozyme and one ho ...201728923715
preliminary results on the efficacy of macroalgal extracts against larvae of aedes albopictus.activity of ethanolic extracts of the algae ulva rigida, asparagopsis taxiformis, dictyota dichotoma, and cystoseira barbata, collected along italian coasts, was tested against larvae of the asian tiger mosquito ( aedes albopictus), a vector of pathogens for animals and humans. tests were carried out using 10 specimens of 3rd-stage larvae per test, following the world health organization standard protocol with minor modifications. among algal extracts, only d. dichotoma was active against the la ...201729369033
quantitative analysis of aedes albopictus movement behavior following sublethal exposure to prallethrin.the pyrethroid prallethrin, an ai in duet™ (clarke mosquito control, st. charles, il), is widely marketed ultra-low volume (ulv) mosquito adulticide. volatilized prallethrin is intended to stimulate mosquito flight, increasing its adulticide effectiveness. however, field tests using volatilized prallethrin have not produced significant differences in mosquito trap catches, leading to questions regarding prallethrin's behavioral impact efficacy. thus, we conducted laboratory tests of prallethrin' ...201729369017
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