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feeding behavior responses of a juvenile hybrid grouper, epinephelus fuscoguttatus♀ × e. lanceolatus♂, to microplastics.in recent decades, microplastic (mp) pollution has become a severe problem in aquatic environments. yet the behavioral and selective responses of fish toward different types of mps remain unclear. we therefore conducted laboratory-based video observations to investigate the behavioral responses of hybrid grouper juveniles (tiger grouper epinephelus fuscoguttatus♀ × giant grouper e. lanceolatus♂) to eight different types of mps. we observed four distinct feeding behaviors: (i) normal ingestion of ...202033070069
identification of anuran species diversity of the panna tiger reserve, central india, using an integrated approach.we present a comprehensive inventory of amphibians from panna tiger reserve in madhya pradesh based on morphological, molecular and bioacoustic data. representatives of 15 anuran species were collected, corresponding to roughly four fifths of the known amphibian species of madhya pradesh. the main results of this study are: (1) description of advertisement calls of eleven species, including the first-time description of advertisement calls of sphaerotheca pashchima. (2) identification of cryptic ...202033056717
three new species of protosticta selys, 1885 (odonata: zygoptera: platystictidae) from the western ghats, india, with taxonomic notes on p. mortoni fraser, 1922 and rediscovery of p. rufostigma kimmins, 1958.three new species of protosticta selys, 1885 (odonata: zygoptera: platystictidae) from the western ghats biodiversity hotspot in india: p. cyanofemora sp. nov. (wet evergreen forests in shendurney wildlife sanctuary, kollam, kerala and kalakkad mundanthurai tiger reserve, tirunelveli, tamil nadu), p. myristicaensis sp. nov. (myristica swamp at kathalekan, shivamogga, karnataka) and p. sholai sp. nov. (montane sholas of upper manalar, meghamalai wildlife sanctuary, theni, tamil nadu) are describe ...202033056228
coronavirus disease 2019 (covid-19) in domestic animals and wildlife: advances and prospects in the development of animal models for vaccine and therapeutic research.sars-cov-2, which causes coronavirus disease 2019 (covid-19), is suspected to have been first contracted via animal-human interactions; it has further spread across the world by efficient human-to-human transmission. recent reports of covid-19 in companion animals (dogs and cats) and wild carnivores such as tigers have created a dilemma regarding its zoonotic transmission. although in silico docking studies, sequence-based computational studies, and experimental studies have shown the possibilit ...202032915100
improving the quality of trauma meetings by implementation of a modern trauma management platform.trauma meetings constitute the majority of clinical decision-making for new referrals and also act as a crucial tool to coordinate the trauma theatre list. incorporation of electronic technology has been shown to improve the quality of patient handover. etrauma is a recently developed cloud-based patient management platform which we planned to implement at our hospital. the aim of our project was to evaluate the quality of our current trauma meeting and subsequently the effect of implementing a ...202032907854
first account of phylogeographic variation, larval characters, and laboratory rearing of the endangered cobblestone tiger beetle cicindelidia marginipennis, dejean, 1831 with observations of their natural history.the cobblestone tiger beetle, cicindelidia marginipennis (dejean, 1831) is a north american species specializing in riparian habitats from new brunswick, canada, to alabama in the united states. in the united states, this species is state-listed as threatened or endangered range-wide and periodically receives consideration for federal listing, mostly due to habitat decline. despite its conservation status, intraspecific genetic diversity for this species has not been explored and little is known ...202033081250
dietary exposure and accumulation of per- and polyfluoroalkyl substances alters growth and reduces body condition of post-metamorphic salamanders.per- and polyfluoroalkyl substances (pfas) are contaminants of concern due to their persistence, potential to bioaccumulate, and toxicity. while dietary exposure is the primary route of exposure for terrestrial species, data on dietary pfas uptake and adverse effects are largely restricted to mammals. as such, substantial data gaps exist that hinder ecological risk assessment, including environmentally relevant exposure levels and taxa. using a 30-d laboratory experiment, we examined the effects ...202033077234
a role of newly found auxiliary site in phospholipase a1 from thai banded tiger wasp (vespa affinis) in its enzymatic enhancement: in silico homology modeling and molecular dynamics insights.phospholipase a1 from thai banded tiger wasp (vespa affinis) venom also known as ves a 1 plays an essential role in fatal vespid allergy. ves a 1 becomes an important therapeutic target for toxin remedy. however, established ves a 1 structure or a mechanism of ves a 1 function were not well documented. this circumstance has prevented efficient design of a potential phospholipase a1 inhibitor. in our study, we successfully recruited homology modeling and molecular dynamic (md) simulation to model ...202032784438
[saw tooth cardiomyopathy: how to better diagnose?]with the increasing use of cardiac mri, several cases were described as "sawtooth cardiomyopathy" or "tiger heart". the pathological aspects of these rare forms of myocardial dysplasia, frequently assimilated to non-compaction of the left ventricle, and its prognostic implications remain unclear. we present a case of "sawtooth cardiomyopathy" in a patient with a transient ischemic attack. this article aims to determine, with the other clinical cases in the literature, the mri and echocardiograph ...202032782066
skim-sequencing based genotyping reveals genetic divergence of the wild and domesticated population of black tiger shrimp (penaeus monodon) in the indo-pacific region.the domestication of a wild-caught aquatic animal is an evolutionary process, which results in genetic discrimination at the genomic level in response to strong artificial selection. although black tiger shrimp (penaeus monodon) is one of the most commercially important aquaculture species, a systematic assessment of genetic divergence and structure of wild-caught and domesticated broodstock populations of the species is yet to be documented. therefore, we used skim sequencing (skimseq) based ge ...202032906759
a lipidomic analysis of leaves of esca-affected grapevine suggests a role for galactolipids in the defense response and appearance of foliar symptoms.both qualitative and quantitative changes occur in the lipid composition of vitis vinifera l. tissues, which may compromise the defense response against esca complex disease, a widespread and damaging trunk disease. in this study, a lipidomic analysis of grapevine leaves is conducted to assess how lipid membrane remodeling relates to the emergence and progression of esca foliar symptoms. in total, 208 molecular species (including lipids, four hormones, and some other compounds of the metabolism ...202032899845
the shrimp microsporidian enterocytozoon hepatopenaei (ehp): biology, pathology, diagnostics and control.disease is a major limiting factor in the global production of cultivated shrimp. the microsporidian parasite enterocytozoon hepatopenaei (ehp) was formally characterized in 2009 as a rare infection of the black tiger shrimp penaeus monodon. it remained relatively unstudied until mid-2010, after which infection with ehp became increasingly common in the pacific whiteleg shrimp penaeus vannamei, by then the most common shrimp species farmed in asia. ehp infects the hepatopancreas of its host, cau ...202032882232
geographic mosaic of selection by avian predators on hindwing warning colour in a polymorphic aposematic moth.warning signals are predicted to develop signal monomorphism via positive frequency-dependent selection (+fds) albeit many aposematic systems exhibit signal polymorphism. to understand this mismatch, we conducted a large-scale predation experiment in four countries, among which the frequencies of hindwing warning coloration of the aposematic moth, arctia plantaginis, differ. here we show that selection by avian predators on warning colour is predicted by local morph frequency and predator commun ...202032881319
identification of a growth-associated single nucleotide polymorphism (snp) in cyclin c of the giant tiger shrimp penaeus monodon.the full-length cdna of cyclin c of the giant tiger shrimp penaeus monodon (pmcyc) was isolated by race-pcr. it was 1443 bp in length containing an open reading frame (orf) of 804 bp and 267 deduced amino acids. tissue distribution analysis indicated that pmcyc was more abundantly expressed in ovaries and testes than other tissues of female and male juveniles (p < 0.05). a pair of primers was designed, and an amplification product of 403 bp containing an intron of 123 bp was obtained. polymorphi ...202032780225
protective effect of cyperus esculentus (tiger nut) extract against scopolamine-induced memory loss and oxidative stress in mouse brain.objectives the juice extract of cyperus esculentus commonly known as tiger nuts (tinut) is widely used for its numerous health promoting effects including alleviation of symptoms associated with neurological disorders. herein, we investigated the influence of the aqueous extract of c. esculentus on cognitive disorder and the underlying changes in acetylcholinesterase (ache) activity and oxidative stress biomarkers in mice exposed to scopolamine. methods c. esculentus (50-200 mg/kg) or saline (10 ...202032776896
comparative analysis of microbial community structure and function in the gut of wild and captive amur tiger.it has been well acknowledged that the gut microbiome is important for host health, composition changes in these microbial communities might increase susceptibility to infections and reduce adaptability to environment. reintroduction, as an effective strategy for wild population recovery and genetic diversity maintenance for endangered populations, usually takes captive populations as rewilding resource. while, little is known about the compositional and functional differences of gut microbiota ...202032793154
can zoos ever be big enough for large wild animals? a review using an expert panel assessment of the psychological priorities of the amur tiger (panthera tigris altaica) as a model species.the ecology of large, wide-ranging carnivores appears to make them vulnerable to conservation challenges in the wild and welfare challenges in captivity. this poses an ethical dilemma for the zoo community and supports the case that there is a need to reconsider prevailing management paradigms for these species in captivity. whilst the welfare challenges wide ranging carnivores face have been attributed to reduced ranging opportunities associated with the decreased size of captive habitats, atte ...202032878205
ability of near-infrared spectroscopy and chemometrics to predict the age of mosquitoes reared under different conditions.practical, field-ready age-grading tools for mosquito vectors of disease are urgently needed because of the impact that daily survival has on vectorial capacity. previous studies have shown that near-infrared spectroscopy (nirs), in combination with chemometrics and predictive modeling, can forecast the age of laboratory-reared mosquitoes with moderate to high accuracy. it remains unclear whether the technique has utility for identifying shifts in the age structure of wild-caught mosquitoes. her ...202032228670
the combined effects of temperature and aromatase inhibitor on metamorphosis, growth, locomotion, and sex ratio of tiger frog (hoplobatrachus rugulosus) tadpoles.the tiger frog (hoplobatrachus rugulosus) is widely raised by many farms in southern region of china as an economically edible frog. the growth, development, and sexual differentiation of amphibians are influenced by temperature and steroid hormone level. however, the problem of hormone residues is caused by the addition of exogenous hormones in frog breeding, it is worth considering whether non-sterol aromatase inhibitors can be used instead of hormones.202032219039
aspartic acid isomerization characterized by high definition mass spectrometry significantly alters the bioactivity of a novel toxin from poecilotheria.research in toxinology has created a pharmacological paradox. with an estimated 220,000 venomous animals worldwide, the study of peptidyl toxins provides a vast number of effector molecules. however, due to the complexity of the protein-protein interactions, there are fewer than ten venom-derived molecules on the market. structural characterization and identification of post-translational modifications are essential to develop biological lead structures into pharmaceuticals. utilizing advancemen ...202032218140
β-glucan alleviates the immunosuppressive effects of oxytetracycline on the non-specific immune responses and resistance against vibrio alginolyticus infection in epinephelus fuscoguttatus × epinephelus lanceolatus hybrids.this study was conducted to examine the combinatory effects of β-glucan and oxytetracycline (otc) on hybrid giant tiger groupers (epinephelus fuscoguttatus × epinephelus lanceolatus). in vitro tests, otc significantly reduced superoxide anion production and phagocytic activity in primary head kidney leukocytes. however, this suppressive effect was alleviated by co-treatment with β-glucan. subsequently, feeding trials were performed to investigate the potential immunomodulatory effects of dietary ...202032217140
variation in the microbiota across different developmental stages of aedes albopictus is affected by ampicillin exposure.the microbiota plays an important role in the growth of mosquitoes and the transmission of mosquito-borne pathogens. the effects of changes in aquatic habitats in which mosquitoes live, as one of the major factors closely associated with the microbial communities of mosquitoes, on the microbiota of different developmental stages remain to be elucidated. here, we compared the microbiota of larvae and pupae of aedes albopictus exposed to different ampicillin concentrations and investigated the bac ...202032207564
author correction: transcriptome analyses reveal the synergistic effects of feeding and eyestalk ablation on ovarian maturation in black tiger shrimp.an amendment to this paper has been published and can be accessed via a link at the top of the paper.202032198472
rat volatiles as an attractant source for the asian tiger mosquito, aedes albopictus.aedes albopictus is a vector of dengue, chikungunya, and dirofilariasis. volatile compounds are crucial for mosquitoes to locate their hosts. this knowledge has allowed the identification of attractants derived from human odours for highly anthropophilic mosquito species. in this study, we used rats as a experimental model to identify potential attractants for host-seeking ae. albopictus females. porapak q extracts from immature female rats were more attractive to ae. albopictus females than tho ...202032198359
spatial, temporal, and genetic invasion dynamics of aedes albopictus (diptera: culicidae) in illinois.the spread of the asian tiger mosquito, aedes albopictus skuse, throughout the united states has implications for the transmission potential of vector-borne diseases. we used a 30-yr data set of occurrence records in illinois and developed a hierarchical bayesian model to shed light on the patterns and processes involved in the introduction and expansion along the northern edge of the geographic range of this species. we also collected specimens from 10 locations and sequenced a segment of their ...202032195543
construction and immunogenicity of virus-like particles of feline parvovirus from the tiger.feline panleukopenia, caused by feline parvovirus (fpv), is a highly infectious disease characterized by leucopenia and hemorrhagic gastroenteritis that severely affects the health of large wild felidae. in this study, tiger fpv virus-like particles (vlps) were developed using the baculovirus expression system. the vp2 gene from an infected siberian tiger (panthera tigris altaica) was used as the target gene. the key amino acids of this gene were the same as those of fpv, whereas the 101st amino ...202032188011
bacterial analysis in the early developmental stages of the black tiger shrimp (penaeus monodon).microbial colonization is an essential process in the early life of animal hosts-a crucial phase that could help influence and determine their health status at the later stages. the establishment of bacterial community in a host has been comprehensively studied in many animal models; however, knowledge on bacterial community associated with the early life stages of penaeus monodon (the black tiger shrimp) is still limited. here, we examined the bacterial community structures in four life stages ...202032184459
new candidates for regulated gene integrity revealed through precise mapping of integrative genetic elements.integrative genetic elements (iges) are mobile multigene dna units that integrate into and excise from host bacterial genomes. each ige usually targets a specific site within a conserved host gene, integrating in a manner that preserves target gene function. however, a small number of bacterial genes are known to be inactivated upon ige integration and reactivated upon excision, regulating phenotypes of virulence, mutation rate, and terminal differentiation in multicellular bacteria. the list of ...202032182341
assessment of lethal, sublethal, and transgenerational effects of beauveria bassiana on the demography of aedes albopictus (culicidae: diptera).dengue fever is one of the most rapidly spreading arthropod-borne diseases. diurnal vectorial properties of aedes albopictus contribute to the dispersion of the dengue viruses. frequent and injudicious use of synthetic insecticides led to the evolution of resistant phenotypes in ae. albopictus which necessitates the search for an alternative of current control strategies. developing a long-lasting and environmentally safe tactic based on knowledge of ecology and population dynamics of ae. albopi ...202032168886
trophic transfer and biotransformation of selenium in the mosquito (aedes albopictus) and interactive effects with hexavalent chromium.as an essential micronutrient for animals with a narrow range between essentiality and toxicity, selenium (se) usually coexists with chromium (cr) in contaminated aquatic environments. this study investigated effects of three diets (microcystis aeruginosa, chlorella vulgaris and biofilms) exposed to se or/and cr on aedes albopictus as a vector for the aquatic-terrestrial transfer of se and cr. se(iv)-exposed mosquitoes concentrated se up to 66-fold faster than se(vi)-exposed ones, corresponding ...202032155550
wolbachia strain wau efficiently blocks arbovirus transmission in aedes albopictus.the global incidence of arboviral diseases transmitted by aedes mosquitoes, including dengue, chikungunya, yellow fever, and zika, has increased dramatically in recent decades. the release of aedes aegypti carrying the maternally inherited symbiont wolbachia as an intervention to control arboviruses is being trialled in several countries. however, these efforts are compromised in many endemic regions due to the co-localization of the secondary vector aedes albopictus, the asian tiger mosquito. a ...202032155143
the broad-scale analysis of metals, trace elements, organochlorine pesticides and polycyclic aromatic hydrocarbons in wetlands along an urban gradient, and the use of a high trophic snake as a bioindicator.wetlands and their biodiversity are constantly threatened by contaminant pollution from urbanisation. despite evidence suggesting that snakes are good bioindicators of environmental health, the bioaccumulation of contaminants in reptiles is poorly researched in australia. we conducted the first broad-scale analysis of 17 metals and trace elements, 21 organochlorine pesticides, and 14 polycyclic aromatic hydrocarbons in the sediments (4 samples per site, december 2018) from four wetlands along an ...202032123945
geographic life history differences predict genomic divergence better than mitochondrial barcodes or phenotype.species diversity can be inferred using multiple data types, however, results based on genetic data can be at odds with patterns of phenotypic variation. tiger beetles of the cicindelidiapolitula (leconte, 1875) species complex have been taxonomically problematic due to extreme phenotypic variation within and between populations. to better understand the biology and taxonomy of this group, we used mtdna genealogies and multilocus nuclear analyses of 34,921 snps to elucidate its evolutionary hist ...202032121321
bioactive ingredients in korean cosmeceuticals: trends and research evidence.cosmeceuticals are commonly used in skincare regimens to maintain healthy skin and improve visible signs of aging. in recent years, south korean skincare has gained prominence in the global beauty industry by introducing innovative cosmeceutical products and aesthetic trends.202032100931
effects of ascorbic acid and β-1,3-glucan on survival, physiological response and flesh quality of cultured tiger grouper (epinephelus fuscoguttatus) during simulated transport in water.transport in water is the most common method for achieving high survival rates when transporting cultured fish in china; yet, transport success relies on proper water quality and conditions. this research was designed to explore the effects of ascorbic acid and β-1,3-glucan on survival, physiological responses, and flesh quality of farmed tiger grouper (epinephelus fuscoguttatus) during simulated transport. the transport water temperature for live tiger grouper was 15 °c, which had the highest s ...202032098052
transcriptome analyses reveal the synergistic effects of feeding and eyestalk ablation on ovarian maturation in black tiger shrimp.unilateral eyestalk ablation in the female black tiger shrimp penaeus monodon is commonly employed to induce ovarian maturation. however, the importance of complementing this practice with the provision of live feed supplement (such as polychaetes) has not been emphasized in previous studies. indeed, it has been less emphasized that female broodstock must be fed with live feeds such as polychaetes for this practice to be effective. while the effects of eyestalk ablation have been thoroughly stud ...202032094422
efficacy and spatial extent of yard-scale control of aedes (stegomyia) albopictus (diptera: culicidae) using barrier sprays and larval habitat management.the asian tiger mosquito, aedes (stegomyia) albopictus (skuse), is a peridomestic, container-ovipositing mosquito commonly found throughout the southeastern united states. in the united states, ae. albopictus is typically considered a nuisance pest; however, it is capable of transmitting multiple pathogens. ae. albopictus is an important pest species and the target of numerous mosquito control efforts in the united states. here, we evaluate the effectiveness and spatial extent of ae. albopictus ...202032052026
microsatellite typing of aedes albopictus (diptera: culicidae) populations from germany suggests regular introductions.the global spread of the asian tiger mosquito aedes albopictus is of concern, as this mosquito species constitutes an important vector of a number of emerging pathogens including dengue virus, chikungunya virus and zika virus. since its first appearance in albania (1979) and italy (1990), the species has been reported from more than twenty-five european countries. however, the dispersion process in europe is largely unknown, as information on population genetic structure is lacking, which is rel ...202032045712
aedes albopictus (asian tiger mosquito). 202032037135
spatial self-organisation enables species coexistence in a model for savanna ecosystems.the savanna biome is characterised by a continuous vegetation cover, comprised of herbaceous and woody plants. the coexistence of species in arid savannas, where water availability is the main limiting resource for plant growth, provides an apparent contradiction to the classical principle of competitive exclusion. previous theoretical work using nonspatial models has focussed on the development of an understanding of coexistence mechanisms through the consideration of resource niche separation ...202031862451
[use of components of the transitional care model in geriatric cross-sectoral care in germany-results of a survey].the inclusion of transitional care professionals to improve the sectoral interface management is politically supported. the nine components of the transitional care model (tcm) originating from the usa, are used in a randomized controlled trial within the tiger project, which is funded by the federal joint committee of germany. geriatric patients are accompanied prior to discharge from hospital and up to 12 months after discharge in the home environment.202033140132
sublethal effects of dermal exposure to poly- and perfluoroalkyl substances on postmetamorphic amphibians.studies of the toxicity of poly- and perfluoroalkyl substances (pfas) on amphibians, especially after metamorphosis, are limited. we examined effects of dermal pfas exposure (30 d) on survival and growth of juvenile american toads (anaxyrus americanus), eastern tiger salamanders (ambystoma tigrinum), and northern leopard frogs (rana pipiens). chemicals included perfluorooctanoic acid, perfluorooctane sulfonate (pfos), perfluorohexane sulfonate (pfhxs), and 6:2 fluorotelomer sulfonate (6:2 fts) a ...202032164037
purification of wap domain-containing antimicrobial peptides from green tiger shrimp peaneaus semisulcatus.antimicrobial peptide crustin was isolated and purified from penaeus semisulcatus using sephadox g-100 column gel filtration chromatography. p. semisulcatus crustins was observed as a single band with 14 kda of molecular weight on sds-page and the retention time of 46 min in rp-hplc. circular dichroism spectra of p. semisulcatus crustin showed alpha helices in its secondary structure followed by random coils. crystalline nature and functional groups arrangement were investigated by x-ray diffrac ...202031843546
the invasive asian tiger mosquito aedes albopictus in romania: towards a country-wide colonization?currently, five invasive aedes mosquito species are of concern in europe according to the european centre for disease prevention and control: aedes aegypti, ae. albopictus, ae. atropalpus, ae. japonicus, and ae. koreicus. among these, only ae. albopictus was reported to occur in romania, in bucharest. the aim of this study was to update the knowledge on the distribution of this invasive mosquito species in romania, by investigating new potential locations. monitoring of ae. albopictus was carrie ...202032036439
improved genomic resources for the black tiger prawn (penaeus monodon).world production of farmed crustaceans was 7.8 million tons in 2016. while only making up approximately 10% of world aquaculture production, crustaceans are generally high-value species and can earn significant export income for producing countries. viet nam is a major seafood producing country earning usd 7.3 billion in 2016 in export income with shrimp as a major commodity. however, there is a general lack of genomic resources available for shrimp species, which is challenging to obtain due to ...202032033920
the asian tiger mosquito in brazil: observations on biology and ecological interactions since its first detection in 1986.aedes (stegomyia) albopictus is a mosquito originating from the asian continent, which was detected in the americas in 1985 and brazil in 1986. due to its rapid expansion throughout brazil, this species has already been reported in 26 of the 27 federative units of brazil. in this review, we evaluate some of the biological, epidemiological and ecological characteristics of ae. albopictus through critical analysis of their importance in the pathogen transmission dynamics, since its first record in ...202032027837
staged hybrid single lumen reconstruction (tiger) in management of chronic symptomatic complex type b aortic dissection, techniques, and literature review.endovascular intervention for chronic symptomatic type b aortic dissection (cs-tbad) induces aortic wall stress with negative hemodynamic cardiovascular consequences. cs-tbad risks increased morbidity and mortality due to septum maturation with significant impact on false lumen modulation, and partial lumen thrombosis conveying the worst outcome. the aim of the tiger technique is total aortic remodeling with true lumen expansion, false lumen regression and complete thrombosis, and stabilization ...202032007594
large-scale operational pyriproxyfen autodissemination deployment to suppress the immature asian tiger mosquito (diptera: culicidae) populations.effective suppression of container-inhabiting asian tiger [aedes albopictus (skuse)] (diptera: culicidae) and yellow fever [aedes aegypti (l.)] (diptera: culicidae) mosquitoes presents one of the most intractable problems for modern mosquito control. traditional tools often fail to control populations of these mosquito species, and are prohibitively expensive or have negative environmental impacts. novel approaches and tools are urgently needed for integrated container-inhabiting mosquito manage ...202032006427
who is eating fructose within the aedes albopictus gut microbiota?the asian tiger mosquito aedes albopictus is a major public health concern because of its invasive success and its ability to transmit pathogens. given the low availability of treatments against mosquito-borne diseases, vector control remains the most suitable strategy. the methods used thus far are becoming less effective, but recent strategies have emerged from the study of mosquito-associated microorganisms. although the role of the microbiota in insect biology does not require further proof, ...202031943686
fecal stress, nutrition and reproductive hormones for monitoring environmental impacts on tigers (panthera tigris).non-invasive stress and nutritional hormone analysis in relation to ecological and other biological indices have tremendous potential to address environmental disturbance impacts on wildlife health. to this end, we examined the relation between glucocorticoid (gc) and thyroid (t3) hormone indices of disturbance and nutritional stress in response to acth and tsh challenges in captive tigers, as well as how reproductive hormones vary by sex and reproductive condition. glucocorticoid, thyroid, prog ...202031942242
type material of arctiinae (lepidoptera: erebidae) in the collection of the museo argentino de ciencias naturales bernardino rivadavia (buenos aires, argentina).the museo argentino de ciencias naturales bernardino rivadavia (macn) possesses one of the most important lepidoptera collections in south america. here are deposited several type specimens of lepidoptera, particularly of arctiinae, described by burmeister, berg, snellen, jörgensen, giacomelli, orfila, and forster. this study presents a catalogue with complete information and photographs of most type specimens of arctiinae housed in the macn. additionally, we provide comments on the type materia ...202032230363
the endogenous plasma small rnaome of rheumatoid arthritis.small rna (srna) sequencing has revealed new srna classes beyond micrornas (mirnas). these srnas can regulate genes and act as biomarkers. the aim of this study was to determine if the endogenous plasma srna landscape is altered in patients with rheumatoid arthritis (ra) compared with control subjects and to determine its association with disease-related parameters in ra.202031913579
spectral organization of the compound eye of a migrating nymphalid, the chestnut tiger butterfly parantica sita.several butterflies of family nymphalidae perform long-distance migration. extensive studies of migration in the iconic monarch butterfly danaus plexippus have revealed that vision plays a crucial role in migratory orientation. differences in the migratory patterns of butterflies suggest that not all species are exposed to the same visual conditions and yet, little is known about how the visual system varies across migratory species. here, we used intracellular electrophysiology, dye injection a ...202031900350
chromosomal-level assembly of takifugu obscurus (abe, 1949) genome using third-generation dna sequencing and hi-c analysis.the tetraodontidae family are known to have relatively small and compact genomes compared to other vertebrates. the obscure puffer fish takifugu obscurus is an anadromous species that migrates to freshwater from the sea for spawning. thus the euryhaline characteristics of t. obscurus have been investigated to gain understanding of their survival ability, osmoregulation, and other homeostatic mechanisms in both freshwater and seawater. in this study, a high quality chromosome-level reference geno ...202031887246
investigating the role of urbanisation, wetlands and climatic conditions in nematode parasitism in a large australian elapid snake.tiger snakes (notechis scutatus) in wetlands of south-west western australia (sw wa) are commonly parasitised by the nematode ophidascaris pyrrhus. host-parasite interactions are complex and can potentially be impacted by factors such as urbanisation or climate. we assessed whether urbanisation, distance to wetland sites, and climatic factors have influenced parasitism in tiger snakes from specimens collected over the last century. we dissected 91 museum specimens of tiger snakes across sw wa an ...202031879593
bone microarchitectural analysis using ultra-high-resolution ct in tiger vertebra and human tibia.to reveal trends in bone microarchitectural parameters with increasing spatial resolution on ultra-high-resolution computed tomography (uhrct) in vivo and to compare its performance with that of conventional-resolution ct (crct) and micro-ct ex vivo.202031993864
juvenile myoclonic epilepsy mimic associated with chd2 gene mutation.this paper reports the electroclinical manifestations of an epilepsy syndrome associated with a chromodomain helicase dna-binding protein 2 (chd2) gene mutation with clinical semiology and electroencephalographic (eeg) features consistent with juvenile myoclonic epilepsy (jme). myoclonic and myoclonic-tonic-clonic seizures, as well as generalized 4- to 5-hz high-amplitude spike-wave and polyspike-wave discharges, were well characterized in an adolescent. however, the atypical age of onset, devel ...202031993582
[the asian tiger mosquito (aedes albopictus) as a challenge for the public health service: an example of a risk management strategy adapted to the situation at the local level].case report on risk management for the epidemiology of an infectious disease threat scenario at the municipal level using the example of the asian tiger mosquito (atm) with communication of figures based on experience to stakeholders in the public health service methods: formal and substantive presentation of a list of defined control measures together with site-specific modification and evaluation of efficacy results: based on a timely education campaign, establishment of a population of atm fi ...202031975361
description of erysipelothrix piscisicarius sp. nov., an emergent fish pathogen, and assessment of virulence using a tiger barb (puntigrus tetrazona) infection model.a recently described emergent disease of ornamental fish has been associated with an erysipelothrix species positive for the surface protective antigen (spa) c gene. whole genome sequencing was performed on five spac erysipelothrix isolates from diseased ornamental fish. in addition, these spac erysipelothrix isolates were compared to spaa-, spab- and other spac-positive erysipelothrix species isolated from terrestrial and marine mammals, birds and fish using multi-locus sequence analysis (mlsa) ...202031682217
cxcr4s in teleosts: two paralogous chemokine receptors and their roles in hematopoietic stem/progenitor cell homeostasis.hematopoietic stem/progenitor cells (hspcs) generate the entire repertoire of immune cells in vertebrates and play a crucial role during infection. although two copies of cxc motif chemokine receptor 4 (cxcr4) genes are generally identified in teleosts, the function of teleost cxcr4 genes in hspcs is less known. in this study, we identified two cxcr4 genes from a teleost, ayu (plecoglossus altivelis), named pacxcr4a and pacxcr4b. pacxcr4b was constitutively expressed in ayu hspcs, whereas pacxcr ...202031959734
dietary taurine stimulates the hepatic biosynthesis of both bile acids and cholesterol in the marine teleost, tiger puffer (takifugu rubripes).taurine (tau) plays important roles in the metabolism of bile acids, cholesterol and lipids. however, little relevant information has been available in fish where tau has been identified as a conditionally essential nutrient. the present study aimed to investigate the effects of dietary tau on the metabolism of bile acids, cholesterol and lipids in tiger puffer, which is both an important aquaculture species and a good research model, having a unique lipid storage pattern. an 8-week feeding tria ...202031959268
investigations into the presence of nidoviruses in pythons.pneumonia and stomatitis represent severe and often fatal diseases in different captive snakes. apart from bacterial infections, paramyxo-, adeno-, reo- and arenaviruses cause these diseases. in 2014, new viruses emerged as the cause of pneumonia in pythons. in a few publications, nidoviruses have been reported in association with pneumonia in ball pythons and a tiger python. the viruses were found using new sequencing methods from the organ tissue of dead animals.202031952524
complementarity of empirical and process-based approaches to modelling mosquito population dynamics with aedes albopictus as an example-application to the development of an operational mapping tool of vector populations.mosquitoes are responsible for the transmission of major pathogens worldwide. modelling their population dynamics and mapping their distribution can contribute effectively to disease surveillance and control systems. two main approaches are classically used to understand and predict mosquito abundance in space and time, namely empirical (or statistical) and process-based models. in this work, we used both approaches to model the population dynamics in reunion island of the 'tiger mosquito', aede ...202031951601
use of tiger nut (cyperus esculentus l.) oil emulsion as animal fat replacement in beef burgers.the present study evaluated the replacement of beef fat in beef burgers using a tiger nut (cyperus esculentus l.) oil emulsion, in order to reduce total fat and saturated fatty acids in the studied samples. three formulations were processed: control-100% beef fat; tiger nut 50% (tn50)-50% of beef fat replaced using tiger nut oil emulsion and tiger nut 100% (tn100)-100% of beef fat replaced by tiger nut oil emulsion. the physicochemical parameters were affected after fat replacement. moreover, th ...202031947797
increased overt attention to objects in early deaf adults: an eye-tracking study of complex naturalistic scenes.the study of selective attention in people with profound deafness has repeatedly documented enhanced attention to the peripheral regions of the visual field compared to hearing controls. this finding emerged from covert attention studies (i.e., without eye-movements) involving extremely simplified visual scenes and comprising few visual items. in this study, we aimed to test whether this key finding extends also to overt attention, using a more ecologically valid experimental context in which co ...202031514103
road network intersection density and childhood obesity risk in the us: a national longitudinal study.road intersection density is an important indicator of walkability. the objectives of this study were to examine the trends in intersection density in the us from 2007 to 2011 and assess the associations between intersection density and childhood obesity risk at the state level.202031605806
tiger team: rapid response at the university of missouri.by the end of the 20th century, health care organizations worldwide were recognizing the benefits of a quick response when patients were experiencing a clinical decline and the difficulty in achieving that goal. the university of missouri stat nurse program, developed in 1989, was an early innovation to deliver the "right care" at the "right time" every time. over the years, the stat nurse program evolved and became the core component of a rapid response system. today rapid response at universit ...202031527978
testosterone and semen seasonality for the sand tiger shark carcharias taurus†.understanding the fundamental reproductive biology of a species is the first step toward identifying parameters that are critical for reproduction and for the development of assisted reproductive techniques. ejaculates were collected from aquarium (n = 24) and in situ (n = 34) sand tiger sharks carcharias taurus. volume, ph, osmolarity, sperm concentration, motility, status, morphology, and plasma membrane integrity were assessed for each ejaculate. semen with the highest proportion of motile sp ...202031836894
microplastic contamination in penaeid shrimp from the northern bay of bengal.microplastic pollution has received increased attention recently due to potential threat to marine biota and human health. this study reports microplastic (mp) content in brown shrimp (metapenaeus monocerous) and tiger shrimp (penaeus monodon) inhabiting in the shallow and offshore waters of the northern bay of bengal, bangladesh. gastrointestinal tract (gt) of shrimps (n = 150) were examined for mps following alkali digestion, microscopic observation and chemical analysis by micro-fourier trans ...202031524623
tongue protrusion dystonia in pantothenate kinase-associated neurodegeneration.tongue protrusion dystonia is an uncommon focal dystonia involving the lingual muscles. causes of tongue protrusion dystonia include tardive dystonia, posthypoxic dystonia, neuroacanthocytosis, pantothenate kinase-associated neurodegeneration, and lesch-nyhan syndrome.202031371123
tigers in the sidewall: surgical approaches to excision of lateral deep infiltrating endometriosis.to describe the surgical approaches and excisional techniques used in an extreme case of deep infiltrating endometriosis (die) affecting the lateral pelvic side wall.202031325590
development and validation of a microrna panel to differentiate between patients with rheumatoid arthritis or systemic lupus erythematosus and controls.microrna (mirna) are short noncoding rna that regulate genes and are both biomarkers and mediators of disease. we used small rna (srna) sequencing and machine learning methodology to develop an mirna panel to reliably differentiate between rheumatoid arthritis (ra) or systemic lupus erythematosus (sle) and control subjects.202031092710
corrigendum: phytoplasmas-the "crouching tiger" threat of australian plant pathology.[this corrects the article doi: 10.3389/fpls.2017.00599.].202030402053
a challenging case of patent ductus arteriosus device closure in an adult with unconventional views and catheters.abnormally oriented patent ductus arteriosus is expected in adults, which can lead to difficulties while attempting a device closure. alternate angiographic views like the "right anterior oblique view," "retrograde approach" and in rare cases, as elicited in the following case scenario, special catheters like the "tiger® catheter" can aid in crossing the lesion and completion of the procedure successfully.202029876027
like the eye of the tiger: inpatient psychiatric facility exclusionary criteria and its "knockout" of the emergency psychiatric patient.over 6% of all emergency department (ed) visits in the united states involve primary mental health or behavioral issues. the patients are stabilized in the ed but frequently require admission to an inpatient psychiatric unit or institution for longer term treatment and management. to facilitate this process, an emergency physician (ep) must first "medically clear" the patient as stable for transfer. at present, there is no interdisciplinary consensus regarding the necessary elements of the medic ...202029097857
giardia duodenalis in captive tigers (panthera tigris), palawan bearcats (arctictis binturong whitei) and asian palm civet (paradoxurus hermaphroditus) at a wildlife facility in manila, philippines.this study was conducted to determine the prevalence of giardia duodenalis in captive animals in a wildlife facility. this is the first study conducted in these animals from the facility.202028979344
neurodegeneration with brain iron accumulation: a case report.pantothenate kinase-associated neurodegeneration (pkan) is an autosomal recessive disorder caused by mutation in the pank2 gene. it is characterized by abnormal brain iron accumulation, mainly in the globus pallidus. pkan is included in a group of disorders known as neurodegeneration with brain iron accumulation (nbia). we report a case of atypical pkan with its most characteristic presentation, exhibiting marked psychiatric symptoms, speech disorder and focal dystonia. brain mri has great diagn ...202029213449
evidence for sars-cov-2 infection of animal hosts.covid-19 is the first known pandemic caused by a coronavirus, sars-cov-2, which is the third virus in the family coronaviridae to cause fatal infections in humans after sars-cov and mers-cov. animals are involved in the covid-19 pandemic. this review summarizes the role of animals as reservoirs, natural hosts and experimental models. sars-cov-2 originated from animal reservoir, most likely bats and/or pangolins. anthroponotic transmission has been reported in cats, dogs, tigers, lions and minks. ...202032629960
predicting susceptibility for sars-cov-2 infection in domestic and wildlife animals using ace2 protein sequence homology.the article is presenting a bioinformatics based method predicting susceptibility for sars-cov-2 infection in domestic and wildlife animals. recently, there were reports of cats and ferrets, dogs, minks, golden hamster, rhesus monkeys, tigers, and lions testing for sars-cov-2 rna which indicated for the possible interspecies viral transmission. our method successfully predicted the susceptibility of these animals for contracting sars-cov-2 infection. this method can be used as a screening tool f ...202033034084
molecular characterization of fecal escherichia coli isolated from zoo animals.accredited zoos and animal parks play an important role in animal health research and conservation, providing important insights on matters of public health including zoonotic infectious diseases and antimicrobial resistance (amr). the emergence and spread of amr is a complex phenomenon that jeopardizes human and animal health and also threatens the long-term survival of endangered species. the presence of β-lactamases in clinical isolates is particularly significant as they can jeopardize the e ...202031926511
detection of lion podoplanin using the antitiger podoplanin monoclonal antibody pmab-231.monoclonal antibodies (mabs) that specifically target podoplanin (pdpn), a marker for type i alveolar cells, are needed for immunohistochemical analyses. anti-pdpn mabs are available for many species, including human, mouse, rat, rabbit, dog, cat, bovine, pig, tasmanian devil, alpaca, tiger, whale, goat, horse, bear, and sheep pdpns. however, no antilion pdpn (liopdpn) antibody has been developed. in this study, possible cross-reaction between available anti-pdpn mabs and liopdpn was examined. i ...202032401677
from people to panthera: natural sars-cov-2 infection in tigers and lions at the bronx zoo.despite numerous barriers to transmission, zoonoses are the major cause of emerging infectious diseases in humans. among these, severe acute respiratory syndrome (sars), middle east respiratory syndrome (mers), and ebolaviruses have killed thousands; the human immunodeficiency virus (hiv) has killed millions. zoonoses and human-to-animal cross-species transmission are driven by human actions and have important management, conservation, and public health implications. the current sars-cov-2 pande ...202033051368
divergent influenza-like viruses of amphibians and fish support an ancient evolutionary association.influenza viruses (family orthomyxoviridae) infect a variety of vertebrates, including birds, humans, and other mammals. recent metatranscriptomic studies have uncovered divergent influenza viruses in amphibians, fish and jawless vertebrates, suggesting that these viruses may be widely distributed. we sought to identify additional vertebrate influenza-like viruses through the analysis of publicly available rna sequencing data. accordingly, by data mining, we identified the complete coding segmen ...202032962015
transmission of severe acute respiratory syndrome coronavirus 2 (sars-cov-2) to animals: an updated review.covid-19 caused by a novel severe acute respiratory syndrome coronavirus 2 (sars-cov-2) originated in wuhan (hubei province, china) during late 2019. it has spread across the globe affecting nearly 21 million people with a toll of 0.75 million deaths and restricting the movement of most of the world population during the past 6 months. covid-19 became the leading health, economic, and humanitarian challenge of the twenty-first century. in addition to the considerable covid-19 cases, hospitalizat ...202032957995
the pre-pleistocene fossil thylacinids (dasyuromorphia: thylacinidae) and the evolutionary context of the modern thylacine.the thylacine is popularly used as a classic example of convergent evolution between placental and marsupial mammals. despite having a fossil history spanning over 20 million years and known since the 1960s, the thylacine is often presented in both scientific literature and popular culture as an evolutionary singleton unique in its morphological and ecological adaptations within the australian ecosystem. here, we synthesise and critically evaluate the current state of published knowledge regardi ...201931534836
widespread cis-regulatory convergence between the extinct tasmanian tiger and gray wolf.the extinct marsupial tasmanian tiger, or thylacine, and the eutherian gray wolf are among the most widely recognized examples of convergent evolution in mammals. despite being distantly related, these large predators independently evolved extremely similar craniofacial morphologies, and evidence suggests that they filled similar ecological niches. previous analyses revealed little evidence of adaptive convergence between their protein-coding genes. thus, the genetic basis of their convergence i ...201931533979
construction of an efficient genomic editing system with crispr/cas9 in the vector mosquito aedes albopictus.aedes (stegomyia) albopictus, also known as the asian tiger mosquito, is a mosquito which originated in asia. in recent years, it has become increasingly rampant throughout the world. this mosquito can transmit several arboviruses, including dengue, zika and chikungunya viruses, and is considered a public health threat. despite the urgent need of genome engineering to analyze specific gene functions, progress in genetical manipulation of ae. albopictus has been slow due to a lack of efficient me ...201930311353
the invasive mosquito aedes albopictus (diptera, culicidae) firstly recorded in bohemia, czech republic.in 2016-2017, the monitoring of possible introduction of an invasive mosquito species, the asian tiger mosquito aedes (stegomyia) albopictus (skuse, 1894) (diptera, culicidae), was conducted in eastern, southern, central and western parts of bohemia, czech republic.201930157658
development of a degree-day model to predict egg hatch of aedes albopictus.aedes albopictus, the asian tiger mosquito, is an important nuisance mosquito species and known vector of arboviruses such as dengue, chikungunya, and zika. despite their cosmopolitan distribution around the world, there is a paucity of accurate predictive models based on rates of development at different temperatures (degree-day models). these types of models can benefit mosquito control districts by predicting when to target early-season larval development, when populations are likely at their ...201931922937
high genetic diversity but no geographical structure of aedes albopictus populations in réunion island.in recent years, the asian tiger mosquito aedes albopictus has emerged as a species of major medical concern following its global expansion and involvement in many arbovirus outbreaks. on réunion island, ae. albopictus was responsible for a large chikungunya outbreak in 2005-2006 and more recently an epidemic of dengue which began at the end of 2017 and is still ongoing at the time of writing. this dengue epidemic has seen a high number of human cases in south and west coastal regions, while few ...201931856896
patterns of spatial genetic structures in aedes albopictus (diptera: culicidae) populations in china.the asian tiger mosquito, aedes albopictus, is one of the 100 worst invasive species in the world and the vector for several arboviruses including dengue, zika and chikungunya viruses. understanding the population spatial genetic structure, migration, and gene flow of vector species is critical to effectively preventing and controlling vector-borne diseases. little is known about the population structure and genetic differentiation of native ae. albopictus in china. the aim of this study was to ...201931752961
high-throughput genotyping of a full voltage-gated sodium channel gene via genomic dna using target capture sequencing and analytical pipeline monas to discover novel insecticide resistance mutations.in insects, the voltage-gated sodium channel (vgsc) is the primary target site of pyrethroid insecticides. various amino acid substitutions in the vgsc protein, which are selected under insecticide pressure, are known to confer insecticide resistance. in the genome, the vgsc gene consists of more than 30 exons sparsely distributed across a large genomic region, which often exceeds 100 kbp. due to this complex genomic structure, it is often challenging to genotype full coding nucleotide sequences ...201931738756
juvenile hormone iii but not 20-hydroxyecdysone regulates the embryonic diapause of aedes albopictus.diapause is an alternative developmental trajectory allowing insects to enter dormancy and persist through predictable periods of seasonally unfavorable conditions. this crucial ecological adaptation defines the geographic and seasonal abundance of many insect pollinators, pests, and vectors. understanding the hormonal changes by which insects coordinate the perception of external, diapause-inducing cues with the physiological mechanisms that lead to developmental arrest is a long-standing goal ...201931708801
differentiation of long non-coding rna and mrna expression profiles in male and female aedes albopictus.the asia tiger mosquito (aedes albopictus) is an important vector of arboviruses, and females can transmit pathogens such as the dengue, zika, and chikungunya viruses. understanding sex-related differences in this mosquito is fundamental for vector control. however, there are no reports of systematic characterization of long non-coding rnas (lncrnas) in male and female ae. albopictus. to investigate the roles of coding and non-coding rnas in both sexes of ae. albopictus, rna sequencing was perfo ...201931681418
pathogenicity and transgenerational effects of metarhizium anisopliae on the demographic parameters of aedes albopictus (culicidae: diptera).dengue fever is a vector-borne infectious disease that spreads swiftly and threatens human lives in several tropical countries. most of the strategies employed for the control of aedes albopictus (skuse) involve synthetic chemicals. the indiscriminate use of synthetic chemicals has led to the development of resistance and is unsafe for human and environmental health. therefore, there is a need to develop ecologically safe tactics, such as the use of the entomopathogenic fungus (epf) metarhizium ...201931819965
predicting the success of an invader: niche shift versus niche conservatism.invasive species can encounter environments different from their source populations, which may trigger rapid adaptive changes after introduction (niche shift hypothesis). to test this hypothesis, we investigated whether postintroduction evolution is correlated with contrasting environmental conditions between the european invasive and source ranges in the asian tiger mosquito aedes albopictus. the comparison of environmental niches occupied in european and source population ranges revealed more ...201931788205
timing of lambda-cyhalothrin and pyriproxyfen barrier treatments for aedes albopictus (diptera: culicidae) management.barrier applications, treating vegetation and other potential mosquito resting areas with residual pesticides, have become standard practice for commercial pest management professionals offering mosquito control services. these treatments are generally effective in reducing numbers of the asian tiger mosquito, aedes albopictus (skuse). in the temperate climates, adult ae. albopictus populations are naturally eliminated each winter and must be grown from diapausing eggs the following spring. ther ...201930768177
a diverse microbial community supports larval development and survivorship of the asian tiger mosquito (diptera: culicidae).laboratory microcosm experiments were conducted to evaluate effects of bacteria isolated from senescent white oak leaves on the growth and survivorship of larval aedes albopictus (skuse). larvae hatched from surface-sterilized eggs were reared in microcosms containing individual bacterial isolates, combined isolates (porphyrobacter sp., enterobacter asburiae, acidiphilium rubrum, pseudomonas syringae, and azorhizobium caulinodans), a positive control containing a microbial community from an infu ...201930753569
first detection of aedes japonicus in spain: an unexpected finding triggered by citizen science.aedes japonicus is an invasive vector mosquito from southeast asia which has been spreading across central europe since the year 2000. unlike the asian tiger mosquito (aedes albopictus) present in spain since 2004, there has been no record of ae. japonicus in the country until now.201930674335
citronella oil-loaded electrospun micro/nanofibrous matrices as sustained repellency systems for the asian tiger mosquito aedes albopictus.mosquitoes are hematophagous insects of major public health concern, serving as vectors of many diseases. available products for personal protection against mosquitoes lack adequate efficacy and in most cases need to be reapplied or replaced frequently. in recent years, the encapsulation of the active repellents in various matrices has arisen as an alternative method for the development of new-generation repellent systems. the aim of the present study was to explore the potential of functional m ...201930632273
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