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assessment of the function of gonad-specific pmago4 in viral replication and spermatogenesis in penaeus monodon.argonaute family is phylogenetically subdivided into ago and piwi subfamilies that operate either transcriptional or post-transcriptional regulation in association with particular types of small rnas. among the four members of ago subfamily (pmago1-4) found in black tiger shrimp penaeus monodon, pmago4 exhibits gonad-restricted expression and takes part in transposon repression as the piwi subfamily. while pmago1-3 participate in rna interference (rnai)-based mechanism, the role of pmago4 in rna ...202132791174
is it time to consider shreds of epidemiological and environmental evidence associated with high transmission of covid-19?novel coronavirus named covid-19 that emerged in late december from wuhan affected almost the entire globe. recent studies provided new insight into the high transmission of the disease. this review explores the current evidence of epidemiological and environmental factors associated with high transmission of covid-19. even transmission and symptoms found among cats, dogs, ferrets, and tiger suggested low species barrier of the virus. the airborne transmission was found even up to 4 m, and fecal ...202134322402
structural analysis of covid-19 spike protein in recognizing the ace2 receptor of different mammalian species and its susceptibility to viral infection.the pandemic covid-19 was caused by a novel coronavirus-2 (sars-cov-2) that infects humans through the binding of glycosylated sars-cov-2 spike 2 protein to the glycosylated ace2 receptor. the spike 2 protein recognizes the n-terminal helices of the glycosylated metalloprotease domain in the human ace2 receptor. to understand the susceptibility of animals for infection and transmission, we did sequence and structure-based molecular interaction analysis of 16 ace2 receptors from different mammali ...202133552834
teleingestion remote video capsule endoscope.we describe 2 cases in which video capsule endoscopy was performed remotely using teleingestion remote video capsule endoscopy (tiger vce), given limited access to endoscopy during the covid-19 pandemic. the patients ingested the capsocam capsule during a televisit, and the video was subsequently reviewed remotely by the physician. tiger vce was used to diagnose a benign etiology of melena and used to confirm a diagnosis of crohn's disease before the initiation of biologic therapy. these cases d ...202134079843
panthera tigris jacksoni population crash and impending extinction due to environmental perturbation and human-wildlife conflict.the critically endangered malayan tiger (panthera tigris jacksoni), with an estimated population of less than 200 individuals left in isolated rainforest habitats in malaysia, is in an intermediate population crash leading to extinction in the next decade. the population has decreased significantly by illegal poaching, environmental perturbation, roadkill, and being captured during human-wildlife conflicts. forty-five or more individuals were extracted from the wild (four animals captured due to ...202133917373
key components of inflammasome and pyroptosis pathways are deficient in canines and felines, possibly affecting their response to sars-cov-2 infection.sars-cov-2 causes the ongoing covid-19 pandemic. natural sars-cov-2 infection has been detected in dogs, cats and tigers. however, the symptoms in canines and felines were mild. the underlying mechanisms are unknown. excessive activation of inflammasome pathways can trigger cytokine storm and severe damage to host. in current study, we performed a comparative genomics study of key components of inflammasome and pyroptosis pathways in dogs, cats and tigers. cats and tigers do not have aim2 and nl ...202133584656
feline coronavirus and feline infectious peritonitis in nondomestic felid species.feline coronavirus (fcov) is reported worldwide and known to cause disease in domestic and nondomestic felid species. although fcov often results in mild to inapparent disease, a small subset of cats succumb to the fatal, systemic disease feline infectious peritonitis (fip). an outbreak of fip in cheetahs (acinonyx jubatus) in a zoological collection demonstrated the devastating effect of fcov introduction into a naïve group of animals. in addition to cheetahs, fip has been described in european ...202133827157
serologic survey of selected pathogens in free-ranging bengal tigers (panthera tigris tigris) in nepal.serum samples of 11 bengal tigers (panthera tigris tigris) from chitwan national park in nepal, collected between 2011-17, were evaluated for the presence of antibodies to eight diseases commonly investigated in large felids. this initial serologic survey was done to establish baseline information to understand the exposure of nepal's free-ranging tiger population to these diseases. tiger serum samples collected opportunistically during encounters such as translocation, human conflict, and injur ...202133822151
genome-wide identification of aedes albopictus long noncoding rnas and their association with dengue and zika virus infection.the asian tiger mosquito, aedes albopictus (ae. albopictus), is an important vector that transmits arboviruses such as dengue (denv), zika (zikv) and chikungunya virus (chikv). long noncoding rnas (lncrnas) are known to regulate various biological processes. knowledge on ae. albopictus lncrnas and their functional role in virus-host interactions are still limited. here, we identified and characterized the lncrnas in the genome of an arbovirus vector, ae. albopictus, and evaluated their potential ...202133481791
sylvatic canine morbillivirus in captive panthera highlights viral promiscuity and the need for better prevention strategies.canine distemper virus (cdv) is a multi-host morbillivirus that infects virtually all carnivora and a few non-human primates. here we describe a cdv outbreak in an exotic felid rescue center that led to the death of eight felids in the genus panthera. similar to domestic dogs and in contrast to previously described cdv cases in panthera, severe pneumonia was the primary lesion and no viral antigens or cdv-like lesions were detected in the central nervous system. four tigers succumbed to opportun ...202133946447
exposure of wild sumatran tiger (panthera tigris sumatrae) to canine distemper virus.canine distemper virus (cdv) is recognized as a conservation threat to amur tigers (panthera tigris altaica) in russia, but the risk to other subspecies remains unknown. we detected cdv neutralizing antibodies in nine of 21 wild-caught sumatran tigers (42.9%), including one sampled on the day of capture, confirming exposure in the wild.202133822169
profile of small rnas, vdna forms and viral integrations in late chikungunya virus infection of aedes albopictus mosquitoes.the asian tiger mosquito aedes albopictus is contributing to the (re)-emergence of chikungunya virus (chikv). to gain insights into the molecular underpinning of viral persistence, which renders a mosquito a life-long vector, we coupled small rna and whole genome sequencing approaches on carcasses and ovaries of mosquitoes sampled 14 days post chikv infection and investigated the profile of small rnas and the presence of vdna fragments. since aedes genomes harbor nonretroviral endogenous viral e ...202133806250
cerebral cysticercosis in a wild bengal tiger (panthera tigris tigris) in bhutan: a first report in non-domestic felids.the endangered bengal tiger (panthera tigris tigris) is a keystone species playing an essential role in ecology as well as in the social and spiritual lives of the himalayan people. the latest estimate of the bengal tiger population in bhutan accounts for 103 individuals. infectious organisms, including zoonotic parasites causing high burden in human health, have received little attention as a cause of mortality in tigers. taeniosis/cysticercosis, caused by the cestode taenia solium, is consider ...202133665082
evaluating angiotensin-converting enzyme 2-mediated sars-cov-2 entry across species.the severe acute respiratory syndrome coronavirus 2 (sars-cov-2) pandemic represents a global threat, and the interaction between the virus and angiotensin-converting enzyme 2 (ace2), the primary entry receptor for sars-cov-2, is a key determinant of the range of hosts that can be infected by the virus. however, the mechanisms underpinning ace2-mediated viral entry across species remains unclear. using infection assay, we evaluated sars-cov-2 entry mediated by ace2 of 11 different animal species ...202133610551
sars cov-2 spike protein in silico interaction with ace2 receptors from wild and domestic species.the severe acute respiratory syndrome coronavirus 2 (sars-cov-2) has been declared a pandemic by the world health organization (who), and since its first report, it has become a major public health concern. sars-cov-2 is closely related to sars-cov and sars-related bat coronaviruses, and it has been described to use angiotensin-converting enzyme 2 (ace2) as a receptor. natural sars-cov-2 infection in domestic and wildlife animals, measured by rt-qpcr, has been confirmed in different countries, e ...202133659022
in vitro characterization of fitness and convalescent antibody neutralization of sars-cov-2 cluster 5 variant emerging in mink at danish farms.in addition to humans, severe acute respiratory syndrome coronavirus 2 (sars-cov-2) can transmit to animals that include hamsters, cats, dogs, mink, ferrets, tigers, lions, cynomolgus macaques, rhesus macaques, and treeshrew. among these, mink are particularly susceptible. indeed, 10 countries in europe and north america reported sars-cov-2 infection among mink on fur farms. in denmark, sars-cov-2 spread rapidly among mink farms and spilled-over back into humans, acquiring mutations/deletions wi ...202134248922
sars-cov-2 in animals: potential for unknown reservoir hosts and public health implications.severe acute respiratory syndrome coronavirus 2 (sars-cov-2, previously 2019-ncov) is suspected of having originated in 2019 in china from a coronavirus infected bat of the genus rhinolophus. following the initial emergence, possibly facilitated by a mammalian bridge host, sars-cov-2 is currently transmitted across the globe via efficient human-to-human transmission. results obtained from experimental studies indicate that animal species such as cats, ferrets, raccoon dogs, cynomolgus macaques, ...202133892621
bats, pangolins, minks and other animals - villains or victims of sars-cov-2?coronavirus disease-19 (covid-19) is caused by the severe acute respiratory syndrome coronavirus-2 (sars-cov-2), which has become unstoppable, spreading rapidly worldwide and, consequently, reaching a pandemic level. this review aims to provide the information available so far on the likely animal origin of sars-cov-2 and its possible hosts/reservoirs as well as all natural animal infections and experimental evidence using animal models. horseshoe bats from the species rhinolophus affinis seem t ...202133464439
sars-cov-2 infection and longitudinal fecal screening in malayan tigers (panthera tigris jacksoni), amur tigers (panthera tigris altaica ), and african lions (panthera leo krugeri) at the bronx zoo, new york, usa.severe acute respiratory syndrome coronavirus-2 (sars-cov-2) emerged as the cause of a global pandemic in 2019-2020. in march 2020, new york city became the epicenter in the united states for the pandemic. on 27 march 2020, a malayan tiger (panthera tigris jacksoni) at the bronx zoo in new york city developed a cough and wheezing with subsequent inappetence. over the next week, an additional malayan tiger and two amur tigers (panthera tigris altaica) in the same building and three lions (panther ...202133480553
evaluation of a real-time rt-pcr panel for detection of sars-cov-2 in bat guano.severe acute respiratory syndrome coronavirus 2 (sars-cov-2) is the causative agent of coronavirus disease 2019 (covid-19), which is an ongoing global health concern. the exact source of the virus has not been identified, but it is believed that this novel coronavirus originated in animals; bats in particular have been implicated as the primary reservoir of the virus. sars-cov-2 can also be transmitted from humans to other animals, including tigers, cats, and mink. consequently, infected people ...202133522461
a literature review of host feeding patterns of invasive aedes mosquitoes in europe.aedes invasive mosquitoes (aims) play a key role as vectors of several pathogens of public health relevance. four species have been established in europe, including aedes aegypti, aedesalbopictus, aedes japonicus and aedes koreicus. in addition, aedes atropalpus has been repeatedly recorded although it has not yet been established. in spite of their importance in the transmission of endemic (e.g., heartworms) and imported pathogens (e.g., dengue virus), basic information of parameters affecting ...202033260438
mutational spectra of sars-cov-2 isolated from animals.coronaviruses are ubiquitous and infect a wide spectrum of animals and humans. the newly emerged severe acute respiratory syndrome coronavirus-2 (sars-cov-2) has become a worldwide pandemic. to address the role that animals may play in the evolution of sars-cov-2, the full genome sequences of sars-cov-2 isolated from animals were compared with sars-cov-2 human isolates from the same clade and geographic region. phylogenetic analysis of sars-cov-2 isolated from the cat, dog, mink, mouse, and tige ...202033384909
progress in source tracking of sars-cov-2.coronavirus disease 2019 (covid-19) pandemic has caused a total of 55 928 327 confirmed cases and 1 344 003 deaths as of november 19, 2020. but so far the origin of severe acute respiratory syndrome coronavirus 2 (sars-cov-2) that causes this pandemic has remained undetermined. the purpose of this study is to review the current research of sars-cov-2 and the existing problems therein, which may provide inspiration for further researches. existing evidence suggested that sars-cov-2 may be derived ...202033380405
an overview of sars-cov-2 and animal infection.a novel coronavirus has been reported as the causative pathogen of the coronavirus disease 2019 (covid-19) outbreak in wuhan city, china in december 2019. due to the rapid spread of the virus worldwide, it has been announced as a pandemic by the world health organization (who). hospitalized patients in wuhan were associated with the huanan seafood wholesale market where live animals, such as poultry, bats, snakes, frogs, rabbits, marmots, and hedgehogs are sold in that market which suggests a po ...202033363234
distemper, extinction, and vaccination of the amur tiger.canine distemper virus (cdv) has recently emerged as an extinction threat for the endangered amur tiger (panthera tigris altaica). cdv is vaccine-preventable, and control strategies could require vaccination of domestic dogs and/or wildlife populations. however, vaccination of endangered wildlife remains controversial, which has led to a focus on interventions in domestic dogs, often assumed to be the source of infection. effective decision making requires an understanding of the true reservoir ...202033229566
tremor-dominant pantothenate kinase-associated neurodegeneration.neurodegeneration with brain iron accumulation (nbia) includes a rare and heterogeneous group of disorders characterized by iron deposition in the basal ganglia. pantothenate kinase-associated neurodegeneration (pkan) is the most common nbia and has 2 main presentations: typical and atypical, the latter rarely presents with tremor. our reported patients underwent full neurologic examination, standard brain magnetic imaging, and genetic testing for pkan. three patients who had "tremor-dominant" p ...202030838286
research on a specialty mushroom (pleurotus tuber-regium) as a functional food: chemical composition and biological activities.pleurotus tuber-regium (ptr) is an edible specialty mushroom that has attracted growing interest recently because of its sensory attributes, high nutritional values, and important medicinal properties. ptr is rich in bioactive polysaccharides, proteins with essential amino acids, essential fatty acids, dietary fiber, minerals, and vitamins. current studies have shown that the nutrients and bioactive ingredients of ptr contribute to their antitumor, antihypercholesterolemic, antihypertensive, ant ...202032786828
a role of newly found auxiliary site in phospholipase a1 from thai banded tiger wasp (vespa affinis) in its enzymatic enhancement: in silico homology modeling and molecular dynamics insights.phospholipase a1 from thai banded tiger wasp (vespa affinis) venom also known as ves a 1 plays an essential role in fatal vespid allergy. ves a 1 becomes an important therapeutic target for toxin remedy. however, established ves a 1 structure or a mechanism of ves a 1 function were not well documented. this circumstance has prevented efficient design of a potential phospholipase a1 inhibitor. in our study, we successfully recruited homology modeling and molecular dynamic (md) simulation to model ...202032784438
[saw tooth cardiomyopathy: how to better diagnose?]with the increasing use of cardiac mri, several cases were described as "sawtooth cardiomyopathy" or "tiger heart". the pathological aspects of these rare forms of myocardial dysplasia, frequently assimilated to non-compaction of the left ventricle, and its prognostic implications remain unclear. we present a case of "sawtooth cardiomyopathy" in a patient with a transient ischemic attack. this article aims to determine, with the other clinical cases in the literature, the mri and echocardiograph ...202032782066
improving the quality of trauma meetings by implementation of a modern trauma management platform.trauma meetings constitute the majority of clinical decision-making for new referrals and also act as a crucial tool to coordinate the trauma theatre list. incorporation of electronic technology has been shown to improve the quality of patient handover. etrauma is a recently developed cloud-based patient management platform which we planned to implement at our hospital. the aim of our project was to evaluate the quality of our current trauma meeting and subsequently the effect of implementing a ...202032907854
identification of a growth-associated single nucleotide polymorphism (snp) in cyclin c of the giant tiger shrimp penaeus monodon.the full-length cdna of cyclin c of the giant tiger shrimp penaeus monodon (pmcyc) was isolated by race-pcr. it was 1443 bp in length containing an open reading frame (orf) of 804 bp and 267 deduced amino acids. tissue distribution analysis indicated that pmcyc was more abundantly expressed in ovaries and testes than other tissues of female and male juveniles (p < 0.05). a pair of primers was designed, and an amplification product of 403 bp containing an intron of 123 bp was obtained. polymorphi ...202032780225
a lipidomic analysis of leaves of esca-affected grapevine suggests a role for galactolipids in the defense response and appearance of foliar symptoms.both qualitative and quantitative changes occur in the lipid composition of vitis vinifera l. tissues, which may compromise the defense response against esca complex disease, a widespread and damaging trunk disease. in this study, a lipidomic analysis of grapevine leaves is conducted to assess how lipid membrane remodeling relates to the emergence and progression of esca foliar symptoms. in total, 208 molecular species (including lipids, four hormones, and some other compounds of the metabolism ...202032899845
the shrimp microsporidian enterocytozoon hepatopenaei (ehp): biology, pathology, diagnostics and control.disease is a major limiting factor in the global production of cultivated shrimp. the microsporidian parasite enterocytozoon hepatopenaei (ehp) was formally characterized in 2009 as a rare infection of the black tiger shrimp penaeus monodon. it remained relatively unstudied until mid-2010, after which infection with ehp became increasingly common in the pacific whiteleg shrimp penaeus vannamei, by then the most common shrimp species farmed in asia. ehp infects the hepatopancreas of its host, cau ...202032882232
geographic mosaic of selection by avian predators on hindwing warning colour in a polymorphic aposematic moth.warning signals are predicted to develop signal monomorphism via positive frequency-dependent selection (+fds) albeit many aposematic systems exhibit signal polymorphism. to understand this mismatch, we conducted a large-scale predation experiment in four countries, among which the frequencies of hindwing warning coloration of the aposematic moth, arctia plantaginis, differ. here we show that selection by avian predators on warning colour is predicted by local morph frequency and predator commun ...202032881319
can zoos ever be big enough for large wild animals? a review using an expert panel assessment of the psychological priorities of the amur tiger (panthera tigris altaica) as a model species.the ecology of large, wide-ranging carnivores appears to make them vulnerable to conservation challenges in the wild and welfare challenges in captivity. this poses an ethical dilemma for the zoo community and supports the case that there is a need to reconsider prevailing management paradigms for these species in captivity. whilst the welfare challenges wide ranging carnivores face have been attributed to reduced ranging opportunities associated with the decreased size of captive habitats, atte ...202032878205
host associated mixed probiotic bacteria induced digestive enzymes in the gut of tiger shrimp penaeus monodon.the shrimp penaeus monodon was used for the isolation of digestive enzyme producing host-associated probiotic bacteria. gut was isolated from a healthy animal completely and morphologically different bacterial isolates were screened for the production of hydrolytic enzymes, such as, protease, amylase, lipase and cellulases. based on their ability to produce enzymes, the potent probiotic bacteria were identified as bacillus subtilis and b. licheniformis and these two were used for the preparation ...202032874122
protective effect of cyperus esculentus (tiger nut) extract against scopolamine-induced memory loss and oxidative stress in mouse brain.objectives the juice extract of cyperus esculentus commonly known as tiger nuts (tinut) is widely used for its numerous health promoting effects including alleviation of symptoms associated with neurological disorders. herein, we investigated the influence of the aqueous extract of c. esculentus on cognitive disorder and the underlying changes in acetylcholinesterase (ache) activity and oxidative stress biomarkers in mice exposed to scopolamine. methods c. esculentus (50-200 mg/kg) or saline (10 ...202032776896
hook-shaped enterolith and secondary cachexia in a free-living grey nurse shark (carcharias taurus, rafinesque 1810).the carcass of a critically endangered, juvenile female grey nurse shark (carcharias taurus, rafinesque 1810) was recovered from a south-eastern australian beach and subjected to necropsy. the 1.98-m-long shark exhibited advanced cachexia with its total weight (19.0 kg) and liver weight (0.37 kg) reduced by 60% and 89%, respectively, compared with a healthy individual of the same length. marked tissue decomposition was evident preventing histopathology and identification of a definitive cause of ...202032776458
transcriptional variation of sensory-related genes in natural populations of aedes albopictus.the asian tiger mosquito, aedes albopictus, is a highly dangerous invasive vector of numerous medically important arboviruses including dengue, chikungunya and zika. in four decades it has spread from tropical southeast asia to many parts of the world in both tropical and temperate climes. the rapid invasion process of this mosquito is supported by its high ecological and genetic plasticity across different life history traits. our aim was to investigate whether wild populations, both native and ...202032767966
evaluation of an in-house indirect enzyme-linked immunosorbent assay of feline panleukopenia vp2 subunit antigen in comparison to hemagglutination inhibition assay to monitor tiger antibody levels by bayesian approach.feline panleukopenia virus (fpv) is an etiologic pathogen of feline panleukopenia that infects all members of felidae including tigers (panthera tigris). vaccinations against fpv among wild felid species have long been practiced in zoos worldwide. however, few studies have assessed the tiger immune response post-vaccination due to the absence of a serological diagnostic tool. to address these limitations, this study aimed to develop an in-house indirect enzyme-linked immunosorbent assay (elisa) ...202032762697
turmeric (curcuma longa l.) extract on oxidative stability, physicochemical and sensory properties of fresh lamb sausage with fat replacement by tiger nut (cyperus esculentus l.) oil.this study aimed to evaluate the effect of turmeric extract as natural antioxidant on modified atmosphere-packaged fresh lamb sausages with fat replacement during storage (2 °c). five treatments were prepared: control without antioxidant (cont); with 500 mg/kg sodium erythorbate (e500); and three batches with 250, 500 or 750 mg/kg turmeric extract (t250, t500 and t750), respectively. the proximate composition, ph, color, texture, oxidative stability, sensorial analysis, free fatty acids and vola ...202032846569
a preliminary study of mirror-induced self-directed behaviour on wildlife at the royal belum rainforest malaysia.mirror-induced behaviour has been described as a cognitive ability of an animal to self-direct their image in front of the mirror. most animals when exposed to a mirror responded with a social interactive behaviour such as aggressiveness, exploratory and repetitive behaviour. the objective of this study is to determine the mirror-induced self-directed behaviour on wildlife at the royal belum rainforest, malaysia. wildlife species at the royal belum rainforest were identified using a camera traps ...202032839483
comparative analysis of the bacterial and fungal communities in the gut and the crop of aedes albopictus mosquitoes: a preliminary study.the asian tiger mosquito aedes albopictus is a major pathogen vector and one of the world's most invasive species. in recent years, the study of mosquito-associated microbiota has received growing interest for reducing transmission of mosquito-borne pathogens. most of studies on mosquito microbiota mainly focused on the gut bacteria. however, microorganisms can also colonize other organs and are not restricted to bacteria. in mosquitoes, the crop is the primary storage organ for sugars from the ...202032752163
molecular biology and structure of a novel penaeid shrimp densovirus elucidate convergent parvoviral host capsid evolution.the giant tiger prawn (penaeus monodon) is a decapod crustacean widely reared for human consumption. currently, viruses of two distinct lineages of parvoviruses (pvs, family parvoviridae; subfamily hamaparvovirinae) infect penaeid shrimp. here, a pv was isolated and cloned from vietnamese p. monodon specimens, designated penaeus monodon metallodensovirus (pmmdv). this is the first member of a third divergent lineage shown to infect penaeid decapods. pmmdv has a transcription strategy unique amon ...202032747554
asian tiger mosquito in yucatan peninsula: first record of aedes (stegomyia) albopictus (diptera: culicidae) in campeche, mexico.this report describes the presence of aedes albopictus skuse (diptera: culicidae) in yucatan peninsula and represents the first record of the asian tiger invasive mosquito in campeche state, southeastern mexico. we collected specimens using 11,326 ovitraps put into houses of urban and rural areas, as part of the entomological surveillance by the local ministry of health from january 2019 to february 2020. we found ae. albopictus in five of the 12 municipalities of campeche (san francisco de camp ...202032623458
[intensification by high dose chemotherapy for germ-cell tumors, still an ongoing subject?]more than 80 % of patient with metastatic germ cell tumor are cured with first line chemotherapy. twenty to 30 % of patients will experience relapse or refractory disease with a very poor long-term prognosis. most of them had metastatic germ cell tumors with a poor prognosis according to the international germ cell classification collaborative group (igcccg). the role of treatment intensification by high dose chemotherapy (hdct) followed by stem cell rescue has not been demonstrated yet in the f ...202032620206
out in the open: behavior's effect on predation risk and thermoregulation by aposematic caterpillars.warning coloration should be under strong stabilizing selection but often displays considerable intraspecific variation. opposing selection on color by predators and temperature is one potential explanation for this seeming paradox. despite the importance of behavior for both predator avoidance and thermoregulation, its role in mediating selection by predators and temperature on warning coloration has received little attention. wood tiger moth caterpillars, arctia plantaginis, have aposematic co ...202032760178
development and validation of a rad-seq target-capture based genotyping assay for routine application in advanced black tiger shrimp (penaeus monodon) breeding programs.the development of genome-wide genotyping resources has provided terrestrial livestock and crop industries with the unique ability to accurately assess genomic relationships between individuals, uncover the genetic architecture of commercial traits, as well as identify superior individuals for selection based on their specific genetic profile. utilising recent advancements in de-novo genome-wide genotyping technologies, it is now possible to provide aquaculture industries with these same importa ...202032758142
natural environmental conditions and collaborative efforts provide the secret to success for sand tiger shark carcharias taurus reproduction in aquaria.sand tiger sharks are an iconic large shark species held in aquaria worldwide. they rarely reproduce under managed care, with only seven aquaria reporting limited and sporadic success. for the first time in the americas, a full-term young was born in an aquarium. the young was the result of breeding among a group of sharks purposefully brought together in 2016 for reproduction. sharks were maintained in natural seawater and exposed to natural light and seasonal temperature fluctuations similar t ...202032757468
molecular characterization of b. anthracis isolates from the anthrax outbreak among cattle in karnataka, india.anthrax, a zoonotic disease is caused by the gram positive bacterium bacillus anthracis. during january 2013, an anthrax outbreak among cattle was reported in gundlupet taluk, neighboring bandipur national park and tiger reserve, india. the present study aims at the molecular identification and characterization of 12 b. anthracis isolates from this outbreak by 16s rrna gene sequencing, screening b. anthracis specific prophages and chromosomal markers, protective antigen (pag) gene and canonical ...202032736522
antimicrobial resistance and toxigenic profiles of bacteria isolated from tropical shrimps (farfantepenaeus notialis and penaeus monodon) in cameroun.post-harvest shrimp losses are a big problem due to the proliferation of spoilage bacteria. presence and multiplication of these bacteria promotes the emergence of food-borne diseases. this study was carried out to characterize some spoilage bacteria from tropical brackish water shrimps and black tiger shrimps stored in ambient temperature (25 °c).202032727545
certified "sustainable" palm oil took the place of endangered bornean and sumatran large mammals habitat and tropical forests in the last 30 years.tropical forests inhabited by endangered orangutans, rhinos, tigers, and elephants in south-east asia are threatened by deforestation, including oil palm expansion. certification has been proposed to label sustainable palm oil production. however, from a remotely sensed time-series and imagery analysis (1984-2020), we discovered that most of the currently certified grower supply bases and concessions in sumatra and borneo are located in the 1990s large mammals habitat and in areas that were biod ...202032721759
fluoride-induced testicular degeneration and sperm quality deteriorations: salutary role of cyperus esculentus tubers (tiger nut) extract in animal model.chronic exposure to fluoride causes tissue damage induced by oxidative imbalance, cyperus esculentus (ce) possess anti-inflammatory and immunostimulatory properties. this study focused on salutary role of cyperus esculentus in sodium fluoride (naf) induced testicular degeneration and sperm quality deteriorations.202032703668
hiv-1 protease and reverse transcriptase inhibition by tiger milk mushroom (lignosus rhinocerus) sclerotium extracts: in vitro and in silico studies.lignosus rhinocerus (lr) is an edible mushroom with a variety of medicinal properties such as neurostimulation, immunomodulation, anti-inflammation, anti-oxidation, anti-proliferation, anti-diabetes and especially antiviral activity. human immunodeficiency virus type-1 (hiv-1) needs the hiv-1 protease (pr) and reverse transcriptase (rt) for its replication. therefore, both hiv-1 pr and rt are important targets for antiretroviral drug development.202032695657
population genomics of two invasive mosquitoes (aedes aegypti and aedes albopictus) from the indo-pacific.the arbovirus vectors aedes aegypti (yellow fever mosquito) and ae. albopictus (asian tiger mosquito) are both common throughout the indo-pacific region, where 70% of global dengue transmission occurs. for ae. aegypti all indo-pacific populations are invasive, having spread from an initial native range of africa, while for ae. albopictus the indo-pacific includes invasive populations and those from the native range: putatively, india to japan to southeast asia. this study analyses the population ...202032678817
predicting health with function -  how can biomechanics "ride the tiger"? 202032677344
movement patterns and habitat use of tiger sharks (galeocerdo cuvier) across ontogeny in the gulf of mexico.the tiger shark (galeocerdo cuvier) is globally distributed with established coastal and open-ocean movement patterns in many portions of its range. while all life stages of tiger sharks are known to occur in the gulf of mexico (gom), variability in habitat use and movement patterns over ontogeny have never been quantified in this large marine ecosystem. to address this data gap we fitted 56 tiger sharks with smart position and temperature transmitting tags between 2010 and 2018 and examined sea ...202032667920
[treatment of testicular germ cell tumors relapse].seminomatous (sgct) and non-seminomatous (nsgct) germ cell tumors (gct) are rare but their incidence are increasing. we will discuss different therapeutic strategies in relapse disease: patients with stage i germ cell tumor have an excellent prognosis with a cure rate approaching 98-99 %, whatever the histology and the chosen treatment (surveillance strategy or adjuvant treatment). relapses are observed among 20% of patients with stage i sgct or low risk nsgct and 50 % of patients with high risk ...202032653158
intramyocardial haematoma causing right ventricular outflow obstruction after brown snake (pseudonaja species) envenomation in a dog.a 15-month-old, male neutered staffordshire bull terrier cross was presented to its referring veterinarian collapsed and agonal. he was immediately intubated, manually ventilated, and treatment commenced for presumptive snake envenomation with two vials of tiger/multi-brown snake antivenom (minimum 7000 units/vial). the dog was transferred to a referral hospital intubated. additional diagnostics performed following arrival at the referral hospital included a urine snake venom detection kit test, ...202032643275
using satellites to track indicators of global air pollution and climate change impacts: lessons learned from a nasa-supported science-stakeholder collaborative.the 2018 nasa health and air quality applied science team (haqast) "indicators" tiger team collaboration between nasa-supported scientists and civil society stakeholders aimed to develop satellite-derived global air pollution and climate indicators. this commentary shares our experience and lessons learned. together, the team developed methods to track wildfires, dust storms, pollen counts, urban green space, nitrogen dioxide concentrations and asthma burdens, tropospheric ozone concentrations, ...202032642628
ecological perspective of disease-resistance prevalence in penaeus monodon.the virulent nature of many suddenly evolved highly contagious pathogens is controlled by surroundings' environment and also by the population density in a particular biogeographical region. worldwide, the white spot disease (wsd) caused by the contagious white spot syndrome virus (wssv) has devastated the aquaculture of penaeid shrimp species. wssv is a highly infectious waterborne pathogen. the black tiger shrimp or penaeus monodon had developed resistance potential against wsd with no antibod ...202032634862
icyhot analgesic topical cream limits cardiac injury in rodents.little is known whether topical analgesic creams, whose natural products enter the blood stream after application, affect myocardial infarct size. here we tested whether topical analgesic creams can trigger remote cardioprotection and the mechanism involved. male sprague dawley rats were used for an in vivo rodent model consisting of 30 minutes left anterior descending coronary artery ischemia and 2 hours of reperfusion followed by infarct size assessment. the topical analgesic icyhot, applied t ...202032629175
immunological and antibiofilm property of haemocyanin purified from grooved tiger shrimp (penaeus semisulcatus): an in vitro and in silico approach.haemocyanin (hc) is a non-specific innate immune protein present in the haemolymph of arthropods and molluscs. in the current study, we characterized the structural and immunological properties of hc from grooved tiger shrimp, penaeus semisulcatus. hc was isolated from the haemolymph of p. semisulcatus by gel filtration column chromatography using sephadex g-100. high-performance liquid chromatography of the purified hc emerged as a single peak through a retention time of 3.3 min demonstrating t ...202032592822
incursion pathways of the asian tiger mosquito (aedes albopictus) into australia contrast sharply with those of the yellow fever mosquito (aedes aegypti).understanding pest incursion pathways is critical for preventing new invasions and for stopping the transfer of alleles that reduce the efficacy of local control methods. the mosquitoes aedes albopictus (skuse) and ae. aegypti (linnaeus) are both highly invasive disease vectors, and through a series of ongoing international incursions are continuing to colonize new regions and spread insecticide resistance alleles among established populations. this study uses high-resolution molecular markers a ...202032592440
bioassay and molecular monitoring of insecticide resistance status in aedes albopictus populations from greece, to support evidence-based vector control.aedes albopictus has a well-established presence in southern european countries, associated with recent disease outbreaks (e.g. chikungunya). development of insecticide resistance in the vector is a major concern as its control mainly relies on the use of biocides. data on the species' resistance status are essential for efficient and sustainable control. to date the insecticide resistance status of ae. albopictus populations from greece against major insecticides used in vector control remains ...202032600453
landscape does matter: disentangling founder effects from natural and human-aided post-introduction dispersal during an ongoing biological invasion.environmental features impacting the spread of invasive species after introduction can be assessed using population genetic structure as a quantitative estimation of effective dispersal at the landscape scale. however, in the case of an ongoing biological invasion, deciphering whether genetic structure represents landscape connectivity or founder effects is particularly challenging. we examined the modes of dispersal (natural and human-aided) and the factors (landscape or founders history) shapi ...202032597498
mercury isotopes as tracers of ecology and metabolism in two sympatric shark species.in coastal ecosystems, top predators are exposed to a wide variety of nutrient and contaminant sources due to the diversity of trophic webs within inshore marine habitats. mercury contamination could represent an additional threat to shark populations that are declining worldwide. here we measured total mercury, carbon and nitrogen isotopes, as well as mercury isotopes, in two co-occurring shark species (the bull shark carcharhinus leucas and the tiger shark galeocerdo cuvier) and their potentia ...202032590319
the effect of laser sintering on the microstructure, relative density, and cracking of sol-gel-derived silica thin films.combining sol-gel processing and laser sintering is a promising way for fabricating functional ceramic deposition with high dimensional resolution. in this work, crack-free silica tracks on a silica substrate with a thickness from ~360 nm to ~950 nm, have been obtained by direct exposure to a co2 laser beam. at a fixed scanning speed, the density and microstructures of the silica deposition can be precisely controlled by varying the laser output power. the porosity of the laser-sintered silica t ...202032587411
method standardization for conducting innate color preference studies in different zebrafish strains.the zebrafish has a tetrachromatic vision that is able to distinguish ultraviolet (uv) and visible wavelengths. recently, zebrafish color preferences have gained much attention because of the easy setup of the instrument and its usefulness to screen behavior-linked stimuli. however, several published papers dealing with zebrafish color preferences have contradicting results that underscore the importance of method standardization in this field. different laboratories may report different results ...202032756400
perceptual advantage of animal facial attractiveness: evidence from b-cfs and binocular rivalry.research has shown that attractive human faces enjoy an advantage in both conscious and preconscious processing. here we examined whether this preference for attractiveness is exclusive to human faces by measuring participants' sensitivity to the attractiveness of cat and tiger faces. experiment 1 measured the time taken to break continuous flash suppression (b-cfs), whereas experiment 2 measured the dominant time in binocular rivalry (br). the results showed that attractive cat faces were detec ...202032754100
complete genome sequence of begonia flower breaking virus, a novel member of the genus potyvirus.we determined the complete genomic sequence of begonia flower breaking virus (bfbv), a novel putative member of the genus potyvirus isolated from begonia bowerae cv. 'tiger' plants grown in kunming. the genomic rna comprises 9540 nucleotides (nt), excluding the 3'-terminal poly(a) tail, and contains a typical open reading frame (orf) of potyviruses. the orf consists of 9219 nucleotides and encodes a 3073-amino-acid polyprotein that is predicted to be proteolytically cleaved into 10 mature peptid ...202032504395
knowledge, attitude and practices towards the tiger mosquito aedes albopictus. a questionnaire based survey in lazio region (italy) before the 2017 chikungunya outbreak.the invasion of aedes albopictus has played a major role in the resurgence of mosquito-borne diseases in italy, generating the two largest chikungunya outbreaks in europe (2007, 2017). knowledge, attitude and practice (kap) are important in order to prevent aedes-borne disease transmission, yet so far they have not been assessed. to this scope we used multivariate logistic regression to investigate kap of citizen-to-aedes ecology and transmitted diseases. data were collated by a structured quest ...202032503246
elevational distribution of the asian tiger mosquito, aedes albopictus, in the inland mountain area of nagano and yamanashi prefectures, japan.nagano and yamanashi prefectures of japan comprise an inland mountain area that extends widely north and south, with elevation varying greatly by location. mosquitoes transmitting infectious disease have a diversity of habitats in nagano and yamanashi, and many species can be expected there. however, there have been few reports on mosquito fauna; in particular, little information is available on mosquitoes such as aedes albopictus. the mosquito fauna was investigated to clarify their elevational ...202032497476
per- and polyfluoroalkyl substances (pfass) in blood of captive siberian tigers in china: occurrence and associations with biochemical parameters.per- and polyfluoroalkyl substances (pfass) have been ubiquitously detected in the environment and marine animals. however, little is known about these substances and their associations with health parameters in wild terrestrial mammals. in this study, we determined pfas levels and distribution in the blood of captive siberian tigers in harbin, china, and evaluated potential exposure pathways by daily intake. in addition, for the first time, we explored the associations between serum pfas concen ...202032480004
the rate of vermi-compost accumulation within 'tiger toilets' in india.tiger toilets use a worm-based ecosystem to degrade human waste and have recently been demonstrated as a cost-effective innovation in on-site sanitation. the benefits over traditional pit latrines include slower fill rate, fewer odours, and safer emptying. however, a question remains around how to measure the rate of accumulation of vermi-compost and predict the fill rate into the future. in this study, fifteen tiger toilets of varying installation ages in the villages of jejuri, bhalgudi and wa ...202032597333
monitoring of alien mosquitoes in western austria (tyrol, austria, 2018).mosquitoes are of major importance to human and animal health due to their ability to transmit various pathogens. in europe the role of mosquitoes in public health has increased with the introduction of alien aedes mosquitoes such as the asian tiger mosquito, aedes albopictus; the asian bush mosquito, ae. japonicus; and ae. koreicus. in austria, ae. japonicus has established populations in various regions of the country. aedes albopictus is not known to overwinter in austria, although isolated f ...202032574163
beginning power mobility: an exploration of factors associated with child use of early power mobility devices and parent device preference.describe and compare young children's use of four early power mobility devices and examine associations between child and environmental factors that may influence power mobility use and parent device preference.202032595979
defense against predators incurs high reproductive costs for the aposematic moth arctia plantaginis.to understand how variation in warning displays evolves and is maintained, we need to understand not only how perceivers of these traits select color and toxicity but also the sources of the genetic and phenotypic variation exposed to selection by them. we studied these aspects in the wood tiger moth arctia plantaginis, which has two locally co-occurring male color morphs in europe: yellow and white. when threatened, both morphs produce defensive secretions from their abdomen and from thoracic g ...202032595271
clinical, haematological and biochemical findings in tigers infected by leishmania infantum.a large number of animal species are susceptible to leishmania infantum (kinetoplastida, trypanosomatidae) in endemic areas, including domestic and wild felids such as tigers (panthera tigris). knowledge on the infection of this endangered species is still at its infancy, and therefore this study aims to identify clinical presentation and clinicopathological findings of tigers naturally infected by l. infantum.202032571332
weights of gaur (bos gaurus) and banteng (bos javanicus) killed by tigers in thailand.the primary prey of tigers across much of south-east asia has been depleted, reducing the ability of already limited habitat to support tigers. to better understand the extent to which two of the largest prey species, gaur (bos gaurus) and banteng (bos javanicus), contribute to the tiger's diet, we estimated the average size of these species killed by tigers. this information is needed to more accurately calculate biomass of these species in the tiger's diet and to devise strategies to increase ...202032551089
population density and habitat use of two sympatric small cats in a central indian reserve.despite appreciable advances in carnivore ecology, studies on small cats remain limited with carnivore research in india being skewed towards large cats. small cats are more specialized than their larger cousins in terms of resource selection. studies on small cat population and habitat preference are critical to evaluate their status to ensure better management and conservation. we estimated abundance of two widespread small cats, the jungle cat, and the rusty-spotted cat, and investigated thei ...202032497053
road development in asia: assessing the range-wide risks to tigers.roads are proliferating worldwide at an unprecedented rate, with potentially severe impacts on wildlife. we calculated the extent and potential impacts of road networks across the 1,160,000-km2, 13-country range of the globally endangered tiger (panthera tigris)-a conservation umbrella species. we found that roads were pervasive, totaling 134,000 km across tiger conservation landscapes (tcls), even in tiger priority sites and protected areas. approximately 43% of the area where tiger breeding oc ...202032494684
rapid adaptive evolution of the diapause program during range expansion of an invasive mosquito.in temperate climates, the recurring seasonal exigencies of winter represent a fundamental physiological challenge for a wide range of organisms. in response, many temperate insects enter diapause, an alternative developmental program, including developmental arrest, that allows organisms to synchronize their life cycle with seasonal environmental variation. geographic variation in diapause phenology contributing to local climatic adaptation is well documented. however, few studies have examined ...202032490563
tissue distribution of transcription for 29 lipid metabolism-related genes in takifugu rubripes, a marine teleost storing lipid predominantly in liver.the tissue distribution pattern of lipid is highly diverse among different fish species. tiger puffer has a special lipid storage pattern, storing lipid predominantly in liver. in order to better understand the lipid physiology in fish storing lipid in liver, the present study preliminarily investigated the tissue distribution of transcription for 29 lipid metabolism-related genes in tiger puffer, which are involved in lipogenesis, fatty acid oxidation, biosynthesis and hydrolysis of glycerides, ...202032415410
phylogenetic position of diplostomum spp. from new world herons based on complete mitogenomes, rdna operons, and dna barcodes, including a new species with partially elucidated life cycle.diplostomum ardeae dubois, 1969 has seldom been reported since its description from the great blue heron (ardea herodias l., 1758) in the usa. sequences obtained in this study from the barcode region of cytochrome c oxidase 1 (co1) in diplostomids from black-crowned night heron (nycticorax nycticorax (l., 1758)) in puerto rico matched data from d. ardeae from a. herodias in the type region. we also obtained dna barcodes from morphologically similar diplostomids from a rufescent tiger heron (tigr ...202032472382
complete genome sequence of sars-cov-2 in a tiger from a u.s. zoological collection.this report describes the identification and characterization of severe acute respiratory syndrome coronavirus 2 (sars-cov-2) in a malayan tiger in a u.s. zoo.202032467283
association of salivary cholinesterase with arthropod vectors of disease.acetylcholinesterase (ache) was previously reported to be present in saliva of the southern cattle tick, rhipicephalus (boophilus) microplus (canestrini), with proposed potential functions to 1) reduce acetylcholine toxicity during rapid engorgement, 2) modulate host immune responses, and 3) to influence pathogen transmission and establishment in the host. potential modulation of host immune responses might include participation in salivary-assisted transmission and establishment of pathogens in ...202032459332
formation of functional innexin hemichannels, as well as gap junctional channels, in an insect cell line, nias-aeal-2, derived from asian tiger mosquito aedes albopictus (diptera: culicidae): a partial but significant contribution of innexin 2.in vertebrates, gap junctions and hemichannels consisting of connexins are important cell surface structures for communication with neighboring cells and for the regulation of various cell functions. to date, various gap-junction-related proteins have been found, including innexins in invertebrates and pannexins in vertebrates. significant contributions of gap junctions by innexins and (hemi-)channels by pannexins to numerous functions have been reported. verification of the presence and functio ...202032446763
genome-based development of 15 microsatellite markers in fluorescent multiplexes for parentage testing in captive tigers.as one of the most endangered species, tiger (panthera tigris) inbreeding has become an urgent issue to address. using a microsatellite (short tandem repeat, str) identification system, paternity testing may be helpful to avoid inbreeding in captive breeding programs. in this study, we developed a genome-based identification system named tiger pedigree identification multiplex system (tpi-plex). by analyzing the entire tiger genome, 139,967 str loci were identified and 12.76% of these displayed ...202032411512
safety in radiation oncology: transposition of directive 2013/59/euratom in the spanish radiation oncology departments-recommendations for its adequate and effective application.the official journal of the european union published on january 17, 2014 the council directive 2013/59/euratom of december 5, 2013, which establishes basic safety standards for the protection against the dangers derived from the exposure to ionizing radiation, and should have been transposed to the regulations of the member countries of the european union.202032445036
draft genome sequences of chlamydiales bacterium ste3 and neochlamydia sp. strain acf84, endosymbionts of acanthamoeba spp.chlamydiales bacterium ste3 and neochlamydia sp. strain acf84 are obligate intracellular symbionts of acanthamoeba spp. isolated from the biofilm of a littoral cave wall and gills from striped tiger leaf fish, respectively. we report the draft genome sequences of these two environmental chlamydiae affiliated with the family parachlamydiaceae.202032409535
anticoagulant activity of black snake (elapidae: pseudechis) venoms: mechanisms, potency, and antivenom efficacy.venoms from pseudechis species (australian black snakes) within the elapidae family are rich in anticoagulant pla2 toxins, with the exception of one species (p. porphyriacus) that possesses procoagulant mutated forms of the clotting enzyme factor xa. previously the mechanism of action of the pla2 toxins' anticoagulant toxicity was said to be due to inhibition of factor xa, but this statement was evidence free. we conducted a series of anticoagulation assays to elucidate the mechanism of anticoag ...202032442717
interleukin-17a/f1 from japanese pufferfish (takifugu rubripes) stimulates the immune response in head kidney and intestinal cells.in mammals, interleukin (il)-17a and il-17f, mainly produced by th17 cells, are hallmark inflammatory cytokines that play important roles in the intestinal mucosal immune response. in contrast, three mammalian il-17a and il-17f counterparts (il-17a/f1-3) have been identified in teleosts, and most of their functions have been described in the lymphoid organs. however, their function in the intestinal mucosal immune response is poorly understood. in this study, a recombinant (r) tiger puffer fish ...202032437858
are shed hair genomes the most effective noninvasive resource for estimating relationships in the wild?knowledge of relationships in wild populations is critical for better understanding mating systems and inbreeding scenarios to inform conservation strategies for endangered species. to delineate pedigrees in wild populations, study genetic connectivity, study genotype-phenotype associations, trace individuals, or track wildlife trade, many identified individuals need to be genotyped at thousands of loci, mostly from noninvasive samples. this requires us to (a) identify the most common noninvasiv ...202032551045
rice-shrimp ecosystems in the mekong delta: linking water quality, shrimp and their natural food sources.aquatic ecosystems are used for extensive rice-shrimp culture where the available water alternates seasonally between fresh and saline. poor water quality has been implicated as a risk factor for shrimp survival; however, links between shrimp, water quality and their main food source, the natural aquatic biota inhabiting these ponds, are less well understood. we examined the aquatic biota and water quality of three ponds over an entire year in the mekong delta, vietnam, where the growing season ...202032544687
ketamine for neuropathic pain: a tiger that won't bite? 202032536446
a case of 'tiger heart': a distinct variant of left ventricular non-compaction. 202032529200
hybridization of tiger grouper (epinephelus fuscoguttatus ♀) x giant grouper (epinephelus lanceolatus ♂) using cryopreserved sperm.using ringer solution as extender, the present study examined the protective effect of dimethyl sulphoxide (me2so; 8-12%, v/v) on the cryopreservation of giant grouper (epinephelus lanceolatus) sperm. the cryopreserved sperm was then successfully applied in interspecific hybridization with tiger grouper (e. fuscoguttatus). higher motility (90.56 ± 6.58%) and fertilization rate (69.61 ± 4.83%) was achieved in 10% me2so with ringer solution as extender (dilution ratio 1:1), which should no signifi ...202032511949
chromium, cadmium, lead, and arsenic concentrations in water, vegetables, and seafood consumed in a coastal area in northern vietnam.heavy metal contamination and related risks for the environment and human health are matters of increasing concern.202032435129
the genome of mekong tiger perch (datnioides undecimradiatus) provides insights into the phylogenetic position of lobotiformes and biological conservation.mekong tiger perch (datnioides undecimradiatus) is an ornamental and vulnerable freshwater fish native to the mekong basin in indochina, belonging to the order lobotiformes. here, we generated 121x stlfr co-barcode clean reads and 18x oxford nanopore minion reads and obtained a 595 mb mekong tiger perch genome, which is the first whole genome sequence in the order lobotiformes. based on this genome, the phylogenetic tree analysis suggested that lobotiformes is more closely related to sciaenidae ...202032424221
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