| proceedings: duration of aspirin's effect on human platelet aggregation. | | 1975 | 2691 |
| proceedings: clonazepam-induced changes in 5-hydroxytryptamine (5-ht) metabolism in aminals and man. | | 1975 | 2712 |
| interaction of thermostable direct hemolysin of vibrio parahaemolyticus with human erythrocytes. | the interaction between thermostable direct hemolysin produced by vibrio parahaemolyticus wp-1 and human erythrocytes was studied. the lysis of human erythrocytes by the hemolysin was dependent of temperature and no hemolysis occurred at low temperature (0-4 c), but the hemolysin was adsorbed on human erythrocytes even at low temperature. no hemolysis was observed when antihemolysin antiserum was mixed with the hemolysin and human erythrocytes at zero time. on the other hand, lysis of the cells ... | 1975 | 3160 |
| the limits of informed consent. | the patient, a 59-year-old man, was referred to a psychiatric hospital with what appeared initially to be the signs and symptoms of mental disorder. in hospital a lesion of the brain was diagnosed and surgery was proposed to relieve the condition. the patient, however, during this and subsequent admissions to hospital, refused operation. his refusal to consent was regarded as valid as he seemed to have good insight into his condition. finally, under section 26 of the mental health act, he was tr ... | 1975 | 2780 |
| calcium-potassium-stimulated net potassium efflux from human erythrocyte ghosts. | in the presence of 8 mm external ca++, the k+ permeability of human red cell ghosts increases provided k+ is also present in the medium. this increase does not represent k+/k+ exchange but a stimulation of net k+ efflux. the stimulation is half-maximal at 0.7 +/- 0.15 mm (n=5). at concentrations above 4.0 mm, external k+ inhibits net k+ efflux. similar stimulatory and inhibitory effects of external k were also observed in intact cells after exposure to pb++ or to ca++ in the presence of fluoride ... | 1975 | 2781 |
| proceedings: glutamine synthetase and glutamine gamma-glutamyl transferase in human, canine, and rat kidneys. | | 1975 | 2787 |
| effect of role empathy on human figures drawn by women alcoholics. | drawings by women alcoholics of the self, a murderer, the murderer's victim and victim's parent revealed conscious and unconscious identification with the depicted roles. | 1976 | 2818 |
| bactericidal activity in vitro of lysosomal proteins from human granulocytes. i. effect of reaction time, ph, temperature and protein dose on lysosomal bactericidal activity. | | 1975 | 2822 |
| examination of csf: the probenecid test. its value and limits in the exploration of cerebral monoamines in man. | | 1975 | 2964 |
| ph and the cytotoxicity of fluoride in an animal cell culture system. | to investigate the mechanism for the toxicity of silicate cement as observed in a cell culture system, the effects of ph and fluoride were tested on human epithelial cells (nctc 2544). at ph 7.3, fluoride concentrations from 15 to 25 mug/ml (0.79 to 1.3 mm) had a growth inhibitory effect. when ph of the incubation medium was lowered in the range 7.0 to 6.4, an enhanced cytoxic effect of fluoride was found, and even at 5 to 10 mug/ml growth inhibition occurred. concomitant with the enhanced cytot ... | 1976 | 2967 |
| the effect of beta-blockade on st segment elevation after acute myocardial infarction in man with some experimental observations. | | 1976 | 3097 |
| effect of sodium fluoride in acid solution on dissolution of powdered human enamel. | | 1976 | 3118 |
| isolation and properties of 2 kallikreins from human saliva. | two kallikreins (k-i and k-ii) were purified from mixed, parotid and submandibular human saliva. the kallikreins were separated by chromatography on cm-cellulose. the ph optima of activity were at ph 9.3 and ph 9.6-9.8; km for baee was 1.10-3m and 4.10-3m, respectively. the esterase activity of k-i and k-ii was inhibited by trasylol-like inhibitors, while the plant and synthetic inhibitors of trypsin were uneffective. in dog, rabbit and rat the hypotensive effect of k-ii and its action upon the ... | 1975 | 3025 |
| comparative study of neuraminidases from "diplococcus pneumoniae" and "clostridium perfringens". | neuraminidases have been purified from the culture medium of two microorganisms, one aerobic, diplococcus neumoniae, the other anaerobic, clostridium perfringens. the enzymatic properties of the 2 neuraminidases have been studied (ph optimum; effect of cations; activity toward different substrates: neuraminyllactose, dilactaminyllacto-n-tetraose, gangliosides, alpha1-acid glycoprotein, collocalia glycoprotein, ovine submaxillary mucin, porcin intestinal and human bronchial mucins). | 1975 | 3129 |
| isolation, identification and quantitative determination of lysolecithin in the human gastric juice. | lysolecithin was isolated and identified from the gastric contents of 5 patients with gastric ulcer and erosive and atrophic gastritis. the methods used involved methanolic extration, column chromatography on silica gel and sephadex gel filtration, followed by preparative thin layer chromatography. identification of lysolecithin was verified in one sample by thin layer chromatography and elementary analysis. a method for quantitation of lysolecithin in human gastric fluid for routine clinical an ... | 1975 | 2972 |
| periarteritis nodosa and cancer. apropos of 2 cases. | the authors report two cases of periarteritis nodosa associated with carcinoma. the first case was a man with an anaplastic bronchial carcinoma, the second was a woman with a rectal adenocarcinoma. study of the association of certain collagen diseases with carcinoma was made to emphasize the great rareness of this association. | 1975 | 2979 |
| toxic effects of halogenated anesthetics on human hepatocytes. | | 1975 | 2989 |
| arboviruses isolated from mosquitos and man in surinam. | in the capital of surinam, and in parts of the coastal plain and the savannah belt 47 strains of arbovirus were isolated from mosquito pools and from sentinel mice. most of the strains (51%) were of the mucambo type. the other types were guama, bimiti, una, catu, restan, maru, and kwatta. culex portesi was found to be the principal vector of all but one (maru) types. other vectors found to be carriers of arboviruses were psorophora ferox, mansonia venezuelensis, and aëdes serratus. the highest p ... | 1975 | 3002 |
| some properties of human uterine myoma phosphofructokinase. | | 1975 | 3149 |
| reduction of 3-oxosteroids in human liver microsomes. | the 3alpha- and 3beta-reduction of the following steroids was studied in human liver microsomes: 5alpha-androstane-3,17-dione, 17beta-hydroxy-5alpha-androstan-3-one, 5alpha-pregnane-3,20-dione, 5beta-pregnane-3,20-dione, 3-oxo-5beta-cholanoic acid and 7alpha-hydroxy-5alpha-cholestan-3-one. with nadh as cofactor there was a preferential 3alpha-reduction of the c19- and c21-3-oxo-steroids and a preferential 3beta-reduction of the c24- and c27-3-oxo-steroids. substitution of nadh with nadph influen ... | 1976 | 3082 |
| histochemical and morphological changes in human muscle spindle in upper and lower motor neuron lesions. | the human intrafusal fibers were found to consist of two morphological and three histochemical (atpase reaction) types. two types of nuclear bag fibers were seen. type a showed alkali stable and acid labile and type b showed acid and alkali stable atpase reaction. the nuclear chain fibers showed only alkali stable atpase reaction. in the lower motor neuron atrophy, muscle spindle showed thickening of the capsule, atrophy of the nuclear chain fibers, and splitting of the nuclear bag fibers. atpas ... | 1976 | 3085 |
| oxidation of corticosteroids to steroidal-21-oic acids by human liver enzyme. | an enzyme that oxidizes corticosteroids to acidic metabolites has been purified from postmortem human liver. the most rapidly oxidized substrate was 11-deoxycorticosterone (doc). other corticosteroids were oxidized at rates that were 10% or less of doc. the products of doc oxidation were 3, 20-dioxopregn-4-en-21-oic acid and 20-hydroxy-3-oxopregn-4-en-21-oic acid. the 20-keto acid was the predominant metabolite in all enzyme preparations. keto acid and hydroxy acid were not interconverted. enzym ... | 1976 | 3202 |
| the action of chelating agents on human liver aldehyde dehydrogenase. | human liver aldehyde dehydrogenase was inhibited by aromatic chelating agents. however, structurally related compounds with much lower metal-complexing ability displayed affinities for enzyme essentially equal to those of their respective chelating analogues. inhibition was competitive with respect to the coenzyme. it is suggested that hydrophobic interactions between the inhibitors and the coenzyme-binding site of the enzyme are responsible for the observed effects on activity. | 1975 | 3170 |
| the effect of ph upon human transferrin: selective labelling of the two iron-binding sites. | the influence of ph changes upon the iron-binding properties of transferrin was investigated in the absence of chelating agents. the effects were demonstrated by spectrophotometry, gel filtration, and by studies of the intermolecular transfer of 59fe from transferrin to conalbumin. at ph values below 6.7, diferric transferrin readily loses iron. the monoferric molecule, which is relatively resistant to acid dissociation, is preferentially formed. a temporary reduction of ph provides a simple met ... | 1976 | 3195 |
| purification and properties of cholesterol ester hydrolase from human aortic intima and media. | 1. cholesterol ester hydrolase of human aortic intima and media was isolated and purified about 650-fold with 10-15% recovery of the original activity by sequential precipitation with 35% acetone, gel filtration on sephadex g-75 and deae-cellulose column chromatography. 2. two ph optima of 4.5-5.0 and 7.0-7.5 were consistently observed for the partially purified cholesterol ester hydrolase of human aortic intima and media. 3. in the system used in the present study, the increasing concentration ... | 1976 | 3220 |
| translational control of protein synthesis in stimulated wi-38 fibroblasts. | a cell-free protein synthesis system employing ribosomes from wi-38 human diploid fibroblasts was developed and its optimum mgc12 and kc1 levels and ph value found. the rate at which ribosomes are able to incorporate radioactive leucine into proteins (14cleucine incorporation/10 min/100 mug rrna) and the number of growing peptide chains 3hpuromycinpeptides formed/100 mug rrna) was determined. when confluent monolayers of wi-38 cells were stimulated to proliferate by serum, a transient increase i ... | 1976 | 3222 |
| the effect of ph on incorporation of galactose by a normal human cell line and cell lines from patients with defective galactose metabolism. | incorporation of radioactive galactose into tca-insoluble material of galactosemic fibroblasts is more sensitive to low ph than is the incorporation by normal human fibroblasts. this study was undertaken to determine (1) whether there was any ph which could correct or counteract the galactosemic defect relative to galactose incorporation, and (2) whether the low ph effect was specific for galactose metabolism or whether general cellular metabolism in galactosemic cells was more sensitive to low ... | 1976 | 3512 |
| stability of fluorescent antibody conjugates stored under various conditions. | two experiments were carried out to determine the stability of fluorescent antibody conjugates. in experiment 1, francisella tularemia conjugates in the lyophilized state retained their original staining titer for 1,294 days when stored at 25, 4 to 5, and -20 c; at 37 c the conjugates were stable for at least 65 days. in the liquid state at ph 7.4 and 8.0 these conjugates were stable for 1,294 days at 4 to 5 and -20 c, whereas those stored at 25 c remained stable through days 473 and 160 of stor ... | 1976 | 3516 |
| dopamine-beta-hydroxylase activity in human cerebrospinal fluid. | | 1976 | 3626 |
| enzymes associated with the metabolism of catecholamines, acetylcholine and gaba in human controls and patients with parkinson's disease and huntington's chorea. | | 1976 | 3629 |
| effects of ph during recombination of human erythrocyte membrane apoprotein and lipid. | the recombinates from human red cell membrane proteins and lipids resulting from dialysis of the components in 2-chloroethanol against aqueous buffers from ph2-12 have been studied by density gradient centrifugation, polyacrylamide gel electrophoresis and freeze-fracture electron microscopy. between ph 4 and 10 most of the proteins were found in the recombinates whereas below ph 4 and above ph 10 only part of them were recovered in the lipoprotein band after density gradient centrifugation. at l ... | 1976 | 3225 |
| acid-base disorders in status asthmaticus (author's transl). | in 85 patinets withstatus asthmaticus, the authors have studied the acid-base balance, the blood gas tensions and various humoral parameters. the values were classified into two groups according to the paco2 level: below or equal to 44 torr (group i), higher than 44 torr (group ii). in the 58 cases of group ii, there was a very close positive correlation between paco2 and h + ions, practically the same as that established by brackett et al. 3 in experimental acute hypercapnia in man. on the cont ... | 1975 | 3243 |
| catechol o-methyltransferase in red blood cells of schizophrenic, depressed, and normal human subjects. | catechol o-methyltransferase of lysed human red blood cells was assayed under optimal conditions, using saturating concentrations of the substrates, s-adenosyl-l-methionine and 3-4-dihydroxybenzoic acid. the mean enzyme activity found in 24 normal subjects was 29-2 nmol/hr/ml rbc. the mean activity in blood of 33 female unipolar depressives was not significantly different from normal. however, higher enzyme activities were observed in the blood of 11 schizophrenic patients (38-9 nmol/hr/ml rbc). ... | 1976 | 3250 |
| proceedings: histamine receptors in human skin. | | 1976 | 3253 |
| quenching of tryptophanyl fluorescence of human growth hormone by iodide. | quenching of tryptophanyl fluorescence of human growth hormone by i- followed saturation kinetics and was abolished by kscn. in the presence of 6 m guanidine hydrochloride quenching was linear between 0 to 0.2 m ki. these results suggest that i- quenched the fluorescence of the native hormone by binding at or near the single tryptophanyl residue. quenching by 0.1 m ki decreased exponentially with increasing concentrations of human and bovine growth hormones. acidification did not have a signific ... | 1976 | 3284 |
| prenatal diagnosis of sickle-cell anemia and alpha g philadelphia. study of a fetus also at risk for h b s/beta+-thalassemia. | | 1976 | 3736 |
| effect of tobramycin on urinary gamma-glutamyltransferase activity: studies in a case of renal carcinoma. | gamma-glutamyltransferase activity was studied in a man presenting with recurrent septicemia owing to pyonephrosis and renal carcinoma. increased activity in the urine was ascribable to administration of the aminoglycoside antibiotic, tobramycin. that the renal carcinoma did not contribute to the increased values was confirmed by homogenization and enzyme histochemistry of the tumor. although the activity of this enzyme in serum was greater than normal, this persisted postoperatively, and thus w ... | 1976 | 3298 |
| physiological disposition and metabolism of (3h)bitolterol in man and dog. | the metabolism and disposition of bitolterol, the di-p-toluate ester of n-t-butylarterenol (tba) was studied in man after a single oral dose and in dog after intraduodenal, iv, or oral administration. the mean (+/- se) peak plasma radioactivity in man (dose, 70 mug/kg) was 180 +/- 18 ng equivalents of 3hbitolterol per ml or approximately 11% of the dose, whereas peak plasma radioactivity in dog (dose, 200 mug/kg) was 144 +/- 23 ng equivalents per ml or approximately 4% of the dose. for both man ... | 1976 | 3404 |
| adriamycin metabolism in man. evidence from urinary metabolites. | we studied the human metabolism of adriamycin by isolating and identifying urinary metabolites which retain adriamycin's specific fluorescence properties. metabolites were extracted by adsorption to polystyrene polymeric sorbants, separated on silicic acid columns and purified by thin-layer chromatography. structures were determined by comparative chromatography; infrared, fluorescence, and mass spectroscopy; and enzymatic and chemical degradation. substances identified were adriamycinol, adriam ... | 1976 | 3405 |
| gamm-glutamyl transpeptidase in human nephroblastoma grown in nude mice. | | 1976 | 3427 |
| influence of mono- and multivalent cations on the electrokinetic properties of normal human lymphoid and burkitt lymphoma cells. | various mono- and multivalent cations, at constant ionic strength, affected the surface electrokinetic properties of normal human lymphoid and burkitt lymphoma cells in a manner which reflected more the physicochemical properties and binding affinities of the cation than its valence. the effect was expressed in the changes of the magnitude of the net surface charge and in the shift of the isoelectric point of the surface. these changes were greater at the surface of burkitt's lymphoma cells than ... | 1976 | 3428 |
| post-prandial changes in ph and electrolyte concentration, in the upper jejunum after truncal vagotomy and drainage in man. | the changes in ph and concentration of electrolytes in the jejunal lumen after a hypertonic fluid meal have been studied after truncal vagotomy and drainage, with and without diarrhoea. the results show that, in these respects, there are no specific changes in the jejunal content associated with post-vagotomy diarrhoea, but that these measurements are markedly affected by the completeness of vagotomy, as judged by the insulin test. | 1975 | 3456 |
| characterization of different deoxyribonucleases in human lymphocytes. | four groups of deoxyribonuclease activities from human lymphocytes have been characterized by deoxyribonuclease assay in dna-containing polyacrylamide gels following their separation by disc-electrophoresis. all activities hydrolyse dna endonucleolytically. one neutral deoxyribonuclease found in the cytoplasmic fraction prefers native or uv-irradiated dna over denatured dna as substrate and is a 5'-monoester former. two groups of acid deoxyribonuclease activities are detectable in the nuclear fr ... | 1975 | 3050 |
| influenza and acute respiratory diseases of non-influenza etiology. | | 1975 | 3065 |
| a study of the host selection patterns of the mosquitoes of the kisumu area of kenya. | the results of 12,168 precipitin tests on blood meals of mosquitoes of the kano plain caught by a variety of catching techniques indicate that to gain an accurate overall picture of feeding patterns both the indoor and the outdoor biotope must be sampled. cdc light traps operated inside houses and monkswood type light traps operated under the outside eaves of houses were found to collect larger numbers of blood fed specimens from a wider range of species than battery driven aspirators collecting ... | 1975 | 2997 |
| the use of pluronic polyols in the precipitation of plasma proteins and its application in the preparation of plasma derivatives. | pluronic f-38 was used a precipitant of plasma proteins under varying conditions of ph and polymer concentration. results indicated that marked differences in the solubility of the plasma proteins in f-38 solutions can be appled to the separation of plasma components. the feasibility of the industrial application of this fractionation method was tested in several experiments. conditions were established for the preparation of albumin, human plasma protein fraction (hppf), and immune serum globul ... | 1976 | 2999 |
| effects of aminophylline, imidazole and indomethacin on spontaneous and prostaglandin induced ovarian contractions in vitro. | imidazole, acetylcholine, phenylephrine prostaglandin f2alpha, and methyl prostaglandin f2alpha increased the contractility of guinea pig and human ovaries in vitro. this effect was suppressed by aminophylline. indomethacin inhibited ovarian contractions. the inhibitory effect of indomethacin was reversed by prostaglandin f2alpha or by its methyl derivative. prostaglandin e2 decreased the amplitude and frequency of the spontaneous and prostaglandin f2alpha induced contractions of guinea pig ovar ... | 1975 | 3482 |
| active transport system in human spermatozoa. | the presence of potassium influx into human spermatozoa was investigated through the use of radioactive k42 and rb86. a gradient of potassium and sodium has been found between the spermatozoal midpiece-tail region and seminal plasma. the influx of potassium seemed to correlate with spermatozoal motility. | 1975 | 3484 |
| lubrication and cartilage. | mechanisms of lubrication of human synovial joints have been analysed in terms of the operating conditions of the joint, the synovial fluid and articular cartilage. in the hip and knee during a walking cycle the load may rise up to four times body weight. in the knee on dropping one metre the load may go up to 25 time body weight. the elastic modulus of cartilage is similar to that of the synthetic rubber of a car tyre. the cartilage surface is rough and in elderly specimens the centre line aver ... | 1976 | 3490 |
| on the occurrence of enzymes of ketone-body metabolism in human adipose tissue. | | 1976 | 4072 |
| quaternary conformational changes in human hemoglobin studied by laser photolysis of carboxyhemoglobin. | these experiments indicate that absorbance changes observed at the 425 nm isosbestic point of the hb and hbco following laser photolysis of hbco provide a direct measure of the rates of quaternary conformational changes between rapidly reacting hb (the immediate product of full photolysis) and slowly reacting normal deoxyhemoglobin. hb, first observed by gibson (gibson, q.h. (1959) biochem. j. 71, 293-303), has been interpreted as deoxyhemoglobin remaining in the liganded quaternary conformation ... | 1976 | 3499 |
| kinetic properties of human placental aromatase. application of an assay measuring 3h2o release from 1beta,2beta-3h-androgens. | the rapid and sensitive assay of 1beta,2beta-3h-androgen aromatization by measurement of 3h2o release (thompson, e.a., jr., and siiteri, p.k. (1974) j. biol. chem. 249, 5364-5372) has been analyzed to determine its applicability to initial rate studies. it was found that aromatization is the sole reaction catalyzed by lyophilized placental microsomes that causes a loss of tritium from position 1 or 2 of androstenedione and testosterone. tritium is, however, removed from position 2 of the estroge ... | 1976 | 3502 |
| vaccination of man and animals by the non-parenteral route. isle-of-man, september 1975. | | 1976 | 3506 |
| binding study of tetracyclines to human serum albumin using difference spectrophotometry. | the binding of several tetracyclines to human serum albumin was studied using difference spectrophotometry and a spectrophotometric probe, 2-(4'-hydroxybenzeneazo)benzoic acid. difference spectra observed for the interaction between the probe and human serum albumin were similar to probe-bovine serum albumin spectra but were less intense for a given concentration of probe and did not reach saturation as quickly. difference spectra for the tetracyclines were dependent on the characteristics of th ... | 1976 | 3641 |
| effect of local anesthetics on chloride transport in erythrocytes. | the self-exchange of chloride isotopes across the human erythrocyte membrane was inhibited by tetracaine, benzocaine, and lidocaine. the inhibition by tetracaine was increased at higher ph values, but was not exclusively due to the uncharged form of tetracaine. the inhibition was effective within 5 seconds, was reversible, was non competitive with chloride ions, and was not reversed by calcium ions. these findings indicate that local anesthetics react with the erythrocyte chloride carrier at a s ... | 1975 | 3651 |
| effect of calcium on the membrane potential of amphiuma red cells. | an increase in extracellular ca concentration causes the membrane of giant red cells of the salamander, amphiuma means, to undergo a marked, transient hyperpolarization. this hyperpolarization is caused by an increase in k permeability of the membrane as judged from the k sensitivity of the membrane potential and from the rate of k loss under influence of raised extracellular ca concentration. at constant external ph, the induction of hyperpolarization by increased extracellular ca has a relativ ... | 1976 | 3652 |
| transitory postnatal hemolysis of calf red cells by amino acids. | among the amino acids which can be solubilized to give a concentration of 300 mm at near physiological ph, histidine and proline caused a complete hemolysis of newborn calf but not of adult cow red cells within 20 to 30 minutes at 38 degrees c. while hydroxyproline, valine, and serine resulted in a partial lysis of calf cells, threonine, glutamine, and glycine were ineffective. in this communication, emphasis has been focused on the mode of the lytic process by histidine which was found to be af ... | 1976 | 3653 |
| cathepsins b1 from human fetal membranes. | cathepsins b1 (ec 3.4.22.1) were isolated from fetal membranes of human placenta, i.e. amnion and chorion-decidua. purification of the enzymes was achieved by the freezing-thawing technique, ammonium sulphate fractionation and sephadex gel filtration. cathepsis b1 separated either from amnion or from chorion-decidua exhibited optimum activity at ph 6.2, and an optimum temperature between 42-45 degrees c. they were inhibited by heavy metals, and compounds which react with the thiol groups. isoele ... | 1976 | 4138 |
| experimental single-stage reconstruction of the tendon sheath and flexor tendon (free tendon graft, using a vein for sheath on the flexor tendon in dogs). | in dog experiments the flexor tendons of the 2nd toe together with their tendon sheaths have been extirpated by the authors. then on the place of the tendon sheath complex-resembling the human hand's "no man's land" -for the substitution of the tendon sheath-free inautotransplants with long vein-segments have been grafted in one sitting. resuming the results of the macroscopic and light microscopic examinations carried out in definite times it has been found by the authors that while the vein se ... | 1976 | 3691 |
| comparison of the pa2 of various beta blocking agents. | the drug industry is now putting out specific beta 1 or beta 2 beta-blocking agents. the pa2 of various beta-blocking agents were determined on isolated organs-guinea pig atrium and trachea: practolol and acebutolol were considered as specific beta-1 inhibitors; butoxamine was a specific beta-2 inhibitor, while pindolol, oxprenolol, propranolol and alprenolol were specificity free. the pa2 quantifies the action exerted by an inhibitor. cardioselectivity is expressed by the pa2 left atrium/pa2 tr ... | 1975 | 3766 |
| effects of adrenergic blockade on adipose tissue lipolysis provoked in cats and humans by intravenous perfusion of isoprenaline. | an intravenous infusion of isoprenaline (1 mug/kg/mn during 4 h) elicited a rise in serum free fatty acids (ffa) and glucose in anaesthetised cats. the effect reached a peak in 1 h and was then maintained at a plateau level for the remainder of the infusion. acebutolol and practolol (0,1-10 mg/kg, p.o.) were more potent in reducing the concentration of free fatty acids (ffa) than in lowering glucose. propranolol was roughly equipotent on ffa and glucose. in 6 fasted human volunteers, an intraven ... | 1975 | 3767 |
| force actions transmitted by joints in the human body. | | 1976 | 3785 |
| arylamidase and cathepsin-a activity of normal and dystrophic human muscle. | human skeletal muscle homogenate has been shown to contain enzymes that catalyze the hydrolysis of l-leucyl p-nitroanilide and carbobenzoxyglutamyl-l-tyrosine, known substrates, respectively, for arylamidase and cathepsin a. the muscle arylamidase was found to be inhibited by p-chloromercuribenzoate. addition of co2+ resulted in slight stimulation of its activity. neither ethylenediamine tetraacetate nor thiol compounds had any appreciable effect on the enzyme. when compared to controls, no sign ... | 1976 | 3799 |
| sperm diaphorase: genetic polymorphism of a sperm-specific enzyme in man. | human sperm contains en enzyme with diaphorase activity that appears to be unique to sperm. electrophoretic analysis of the diaphorase activity in sperm of different individuals reveals three phenotypic patterns. this polymorphism can be explained in terms of two alleles segregating at an autosomal locus; the allele frequencies have been determined to be 0.71 and 0.29. this appears to be the first reported example of a sperm-specific genetic polymorphism in man; its existence raises a number of ... | 1976 | 3851 |
| influence of sugar content in soft bread on ph of human dental plaque. | quantitative determination of monosaccharides, disaccharides and sorbitol by use of gas-liquid chromatography (glc) was performed on thirty-three samples of different commercial soft bread. maltose was found in all the bread samples. fructose and glucose were found only in samples of sweetened bread. sucrose was detected in 5 samples, lactose in 2, and sorbitol in 2. up to 20 per cent of the fresh bread weight was found to be low-molecular weight carbohydrates. plaque ph-changes were studied in ... | 1975 | 3941 |
| diamine oxydase in rabbit small intestine: separations from a soluble monoamine oxidase, properties and pathophysiological significance in intestinal ischemia. | from all mammals investigated so far only in rabbits diamine oxidase could not be detected in any tissue except the gut. thus this species was chosen for studying the physiological and pathophysiological function of this enzyme in the gastrointestinal tract. by gel filtration on sephadex g 50 and g 200 the enzyme was purified 100-fold, separated from a soluble monoamine oxidase, and the properties of the two enzymes were determined. diamine oxidase from rabbit small intestine deaminated putrecin ... | 1975 | 3954 |
| the inter-alpha-trypsin inhibitor as precursor of the acid-stable proteinase inhibitors in human serum and urine. | a small amount of antitryptic activity is detectable in the supernatant of deproteinized human serum. preincubation of serum with trypsin causes an increase in acid-stable antitryptic activity. this rise in activity depends on the inter alpha-trypsin inhibitor concentration. the native inhibitor present in normal sera, and in higher concentrations in sera of patients with nephropathies, and the trypsin-liberated inhibitor show immunological cross reaction with antibodies to the serum inter-alpha ... | 1976 | 3463 |
| radioiodination of human low density lipoprotein: a comparison of four methods. | a comparison has been made of four labelling techniques used to radioiodinate human low density lipoprotein (ldl). (1) chloramine t iodination at ph 7.4 was 20-25% efficient and gave a product immunologically indistinguishable from native ldl. approximately 30% of the incorporated radioactivity, however, was found in ldl lipids, and the metabolic decay of the labelled complex in rats did not obey first order kinetics. radiolabelling at ph 10 reduced the uptake of 125i into lipids to 10% but also ... | 1976 | 4242 |
| purification and properties of human acid-thermostable ribonucleases, and diagnosis of childhood pancreatic fibrosis. | acid-thermostable ribonucleases were isolated from human pancreas, duodenal contents, liver, spleen, serum and urine, and purified 15--1000-fold. the ph optima, ionic requirements, and some of the specificity requirements, of these enzymes were investigated. the isolated enzymes formed two distinct groups: (a) the ribonucleases of the pancreas, duodenal contents and fraction a of serum and urine exhibit a ph optimum of 8.5, are inhibited by an2+ and cu2+, and relatively rapidly hydrolyze the syn ... | 1976 | 4243 |
| physico-chemical and immunological properties of acid alpha-glucosidase from various human tissues in relation to glycogenosis type ii (pompe's disease). | the physico-chemical and immunological properties of acid alpha-glucosidase from various human tissues have been studied. heat stability of acid alpha-glucosidase from heart, liver and skeletal muscle is identical, but for kidney some different results are obtained. identical isoelectrofocussing patterns are found for heart, liver and skeletal muscle. furthermore, the effect of antiserum against human liver acid alpha-glucosidase on the activity of acid alpha-glucosidase from various tissues is ... | 1976 | 4245 |
| partial purification and properties of ovine liver echinococcus granulosus protoscolices phospholgucose isomerase. | echinococcus granulosus protoscolex is the actual larval stage of the cestode causing echinococcosis both in man and animals. in the present report, certain properties of phosphoglucose isomerase from the ovine liver e. granulosus protoscolices have been studied and compared with those of the hydatid cyst fluid and the healthy ovine liver enzymes. the protoscolices enzyme prepared in a manner similar to the hydatid cyst fluid and the ovine liver enzymes exhibited the following properties: (1) ph ... | 1976 | 4246 |
| biochemical differences between the mb and mm isoenzymes of creatine kinase. | the biochemical properties of partially purified preparations of human myocardial mb and mm iosenzymes of creatine kinase have been compared with one another. differences in substrate affinity, behaviour with inhibitors, and heat denaturation have been demonstrated. it is doubtful if the differences we have shown are sufficiently great to form the basis of a practical routine procedure for measuring serum levels of the mb isoenzyme. | 1976 | 4247 |
| the intracellular ph of human leucocytes in response to acid-base changes in vitro. | 1. viable human leucocytes were isolated from venous blood and suspended in artificial media. intracellular ph measurements were made by the dimethyloxazolidinedione technique in conditions simulating "respiratory" or "metabolic" acid-base disturbances. 2. normal intracellular ph was 7-11 +/- 0-02 (mean +/- 2 sd) at an extracellular pco2 of 5-8 kpa and a bicarbonate concentration of 25 mmol/l. 3. "respiratory" and "metabolic" acidosis caused little change in phi although increases in pco2 led to ... | 1976 | 4259 |
| marrow transplantation in aplastic anemia and leukemia. | human marrow transplantation has resulted in observations of fundamental significance in understanding both aplastic anemia and acute leukemia. for example, the observation that transplanted marrow can grow successfully in patients with aplastic anemia indicates that the disease is due to a defect in the marrow precursor cells and not in the marrow microenvironment. similarly, the observation of recurrent leukemia in donor cells has important implications. nonetheless, marrow transplantation is ... | 1976 | 4261 |
| fatty acids of the portuguese man-of-war physalia physalis. | | 1976 | 4283 |
| the activator of human cerebroside sulphatase. activating effect on the acidic forms of the sulphatases from invertebrates. | 1) acidic forms of the sulphatase were partially purified from the following invertebrate species: tethya aurantium (porifera), patella vulgata (mollusca), maja squinado (arthropoda), marthasterias glacialis (echinodermata) and microcosmus sulcatus (tunicata). enzyme preparations thus obtained cleaved cerebroside sulphates (sulphatides) only in the presence of either specific detergents (e.g. taurodeoxycholate) or an activator protein isolated from human liver. this corresponds to the findings o ... | 1976 | 3465 |
| absorption activity of the blood group substances in various fractions of split ejaculates from man. | antigens of the abo system have been determined in the various fractions of split ejaculates from three a and two o (h) men who were secretors. the activity of the antigen was determined by a hemagglutination inhibition test. within a split ejaculate the various fractions contained almost the same antigen activity, indicating that the antigens were secreted from all the glands contributing to the seminal plasma. | 1975 | 3477 |
| a neutral collagenase from human gastric mucosa. | biopsy specimens of human gastric mucosa, maintained in culture for 7 days in the absence of serum, released a collagen-degrading enzyme into the medium. the yield of active enzyme reached a maximum after 2-3 days, and viable tissue, capable of protein synthesis, was essential for its production. 2. at 25 degrees c the enzyme attacked undenatured collagen in solution, resulting in a 55% loss of specific viscosity and producing the two products tca and tcb characteristic of neutral-collagenase ac ... | 1976 | 4057 |
| creatine kinase equilibrium and lactate content compared with muscle ph in tissue samples obtained after isometric exercise. | muscle biopsies taken from the musculus quadriceps femoris of man were analysed for ph, atp, adp, amp, creatine phosphate, creatine, lactate and pyruvate. biopsies were taken at rest, after circulatory occlusion and after isometric contraction. muscle ph decreased from 7.09 at rest to 6.56 after isometric exercise to fatigue. decrease in muscle ph was linearly related to accumulation of lactate plus pyruvate. an increase of 22mumol of lactate plus pyruvate per g of muscle resulted in a fall of 0 ... | 1975 | 4060 |
| the availability of folates in man: effect of orange juice supplement on intestinal conjugase. | the mechanisms of the low availability of endogenous folates in orange juice and the inhibitory effects on the availability of pteroylheptaglutamate (pteglu7) caused by orange juice supplements were investigated in man by the use of simulated orange juice (containing the principal components: citric acid, malic acid, etc.) with several different phs. the endogenous folates in orange juice were identified as mainly 5-methyl reduced derivatives of pteroylpolyglutamates (40-45% pentaglutamate, 10-1 ... | 1976 | 4083 |
| an analysis of the autophosphorylation of rabbit and human erythrocyte membranes. | the autophosphorylation of rabbit and human erythrocyte membranes has been studied under various experimental conditions. the phosphopeptides of the erythocyte membranes were identified using sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis followed by ratioautography. the pattern of phosphorylatiion of membrane components differs with respect to the phosphoryl donor used (atp or gtp) and to the ph at which the reaction is carried out. both species appear to contain at least two di ... | 1976 | 4093 |
| the effect of silver ion binding and ph on the buoyant density of dna and its use in fractionating heterogeneous dna. | 1. the effect of ph on the buoyant density of the complexes of ag+ with dna has been studied using 3h-labeled human dna and several bacterial dnas to determine the conditions necessary for the maximum resolution of compositional heterogeneity. in neutral cs2so4 density gradients, ag+ complexes with (g - c)-rich components are always denser than those with (a - t)-rich components, since (g - c)rich dnas have a larger affinity for ag+ than (a - t)-rich dnas and their complexes are denser than (a - ... | 1976 | 4104 |
| binding of norgestrel to human plasma proteins. | binding of 14, 15-3h(+/-)-norgestrel to human plasma proteins has been investigated. norgestrel showed greater affinity to plasma than to human serum albumin indicating specific norgestrel binding protein(s) in the plasma. alpha1-acid glycoprotein showed high affinity for norgestrel when compared with human serum albumin. the binding protein was eluted at ph 5.8 by step by step elution on a deae-cellulose column. norgestrel binding to plasma proteins was not affected at 60 degrees c. the optimal ... | 1976 | 4109 |
| response of the glycolysis of human erythrocytes to the transition from the oxygenated to the deoxygenated state at constant intracellular ph. | the time course of the rate of the glycolysis of human erythrocytes and of some metabolites were determined before and after rapid deoxygenation at constant intracellular ph. for this purpose stripped deoxygenated haemoglobin was used as a rapid oxygen acceptor. deoxygenation causes an increase of the glycolytic rate by 26%. glucose 6-phosphate is decreased while the adenine nucleotides and 2,3-bisphosphoglycerate remain constant. fructose 1,6-bisphosphate and the triose phosphates decrease tran ... | 1976 | 4113 |
| pituitary gonadotropin response to luteinizing hormone-releasing hormone (lh-rh) in males with azo- and oligospermia. | twenty-one males with azo- or oligospermia presenting with infertility and with no evidence of organic disease were studied with luteinizing hormone - releasing hormone (lh-rh). a pituitary luteinizing hormone (lh) and follicle stimulating hormone (fsh) response was present in all cases and was normal in the majority of the subjects. serum testosterone and 17 beta estradiol levels were normal in all cases studied. no significant correlations were found between the gonadotropin estimations and sp ... | 1975 | 4378 |
| galvanic separation of x- and y-bearing human spermatozoa. | | 1975 | 4379 |
| juvenile diabetes and human sperm quality. | spermiological findings in 25 juvenile diabetics in the age range 16 to 22 years (average 18.5) have been compared with normal spermiological findings in 24 individuals of the same age (16 to 22 years, average 18.7). a drift to lower sperm values in the group of the juvenile diabetics became evident in all parameters observed. as concerns motility and morphology, the differences between the groups reached statistically significant values. | 1975 | 4382 |
| on round-headed human spermatozoa. | in an infertile man and his only, likewise infertile, brother the sperm contained only round-headed spermatozoa. electron microscopic examination of embedded ejaculate revealed a malformation, viz. absence of acrosomes in all the spermatozoa with consequent lack of normal development of the heads of the spermatozoa. acrosomeless spermatozoa were also found in the testicular tubuli. no disturbance of the endocrine functions could be demonstrated. the blood cells were of normal male karyotype. inv ... | 1975 | 4383 |
| crossed immunoelectrophoresis of sperm antibodies in human serum and cervical mucus. | sperm agglutinating antibodies are purified from sera and cervical mucus of women with unexplained causes of infertility which were positive in the fd-test. fractionation was performed by affinity-chromatography in a batch device and the sperm agglutinating activity controlled by the franklin and dukes test. this sperm antibody fraction was determined via crossed immunoelectrophoresis by migration into an anti-human serum containing gel. in all cases only one big peak resulted. the negative cont ... | 1975 | 4387 |
| ascorbic acid-2-sulfate sulfhohydrolase activity of human arylsulfatase a. | pure human arylsulfatase a (ec 3.1.6.1) was found to hydrolyze ascorbic acid 2-sulfate to ascorbic acid and inorganic sulfate at rates from 200 to 2000 mumol/mg per h depending on the method of assay. this rate was lower than that observed with the synthetic substrate 4-nitrocatechol sulfate, but higher than that seen with the physiological substrate cerebroside sulfate. extracts of cultured fibroblasts from normal subjects were also shown to hydrolyze ascorbic acid 2-sulfate; extracts of fibrob ... | 1976 | 4134 |
| human alpha-fucosidase. single residual enzymatic form in fucosidosis. | four major forms of alpha-fucosidase (ec 3.2.1.51) activity were separated by isoelectrofocusing from sera of normal control individuals. all forms shifted towards less acidic pi values after neuraminidase treatment. in two patients affected with fucosidosis, only a single major acidic peak was observed and this was affected to a lesser degree by neuraminidase treatment. the kinetics of heat inactivation of the residual activity found in these two patients showed two decay rates while the contro ... | 1976 | 4135 |
| pyruvate flux into resealed ghosts from human erythrocytes. | the kinetics of pyruvate transport across the isolated red blood cell membrane were studied by a simple and precise spectrophotometric method: following the oxidation of nadh via lactate dehydrogenase trapped within resealed ghosts. the initial rate of pyruvate entry was linear. influx was limited by saturation at high pyruvate concentration. pyruvate influx was greatly stimulated by increasing ionic strength in the outer but not the inner aqueous compartment. the km ranged from 15.0 mm at mu = ... | 1976 | 4147 |
| 17 beta-hydroxysteroid dehydrogenase of the sheep ovary : purification, properties and substrate binding site. | sheep ovarian 17 beta hsdh has been purified about 1000 fold to a specific activity of 0.5 iu/mg protein, using deae cellulose chromatography, affinity chromatography on estrone-amino caproate-sepharose and a second deae cellulose chromatography. the molecular weight is 70,000 ; the ph optimum for activity is 9.2 and the energy of activation is 16.5 kcal/mole. the kinetics of the oxidation of estradiol and many analogues have been studied at various concentrations and in the presence of differen ... | 1975 | 4149 |
| studies on gamma-glutamyl transpeptidase in human and rabbit erythrocytes. | gamma-glutamyl transpeptidase transfers the gamma-glutamyl moiety of glutathione to a variety of acceptor amino acids. through the operation of the gamma-glutamyl-cyclotransferase cycle, this enzyme has been implicated in the transport of amino acids into cells, especially the cells of the proximal tubules of kidney. it has been reported to be present in rabbit erythrocytes. however, using white cell-free preparations, we have not been able to demonstrate the presence of gamma-glutamyl transpept ... | 1976 | 4175 |
| isolation of human eosinophil phospholipase d. | phospholipase d preferentially contained in human eosinophil polymorphonuclear leukocytes as compared to other leukocytes was isolated by sequential asion and cation exchange chromatography and gel filtration. the purified eosinophil enzyme specifically liberated choline from i-alpha-phosphatidyl choline with a ph optimum of 4.5-6.0 and exhibited a pi of 5.8-6.2 on polyacrylamide-gel isoelectric focusing, which are properties shared by phospholipase d from plant sources; however, its apparent mo ... | 1976 | 4468 |
| proceedings: inhibition by antigen and by histamine antagonists of the uptake of histamine by isolated human leucocytes. | | 1976 | 4180 |
| histamine h2-receptors in the human peripheral circulation. | histamine (10 mug/min for 3 min) infused into the brachial artery caused an increase in forearm blood flow which was reduced by mepyramine (25 mg). this effect was most marked in the first minute of the infusion. metiamide (25 mg) had no effect on the dilatation during the infusion but caused a quicker return of flow to the resting level. the response was abolished when both drugs were given in combination. it is concluded that the response is initiated mainly by stimulation of h1-receptors and ... | 1976 | 4187 |
| urinary gamma-glutamyl transpeptidase, an indicator of renal ischaemic injury and homograft rejection. | gammaglutamyl transpeptidase is an enzyme primarily located in the brush border of the proximal convoluted tubules of the kidney. its unique localisation in the renal cells most easily damaged by ischaemia and its ease of assay provides the rationale for its use in the measurement of renal ischaemic injury. using a standard experimental animal model, canine urinary gamma-gt activity was shown to be increased up to 70-fold following 90 min of unilateral renal ischaemia and was significantly raise ... | 1975 | 4190 |
| controlled environment culture of bone marrow explants from human myeloma. | bone marrow biopsy specimens from patients with myeloma were cultured in either 1 of 2 thin-film culture systems, a controlled environment steady state system or a rocker tube configuration of the system, for periods up to 42 days. both functional and morphological characteristics of the myeloma cells were well-maintained in these systems. cytocentrifuge preparations of the culture media disclosed hematopoietic cells that included from 5% to almost 100% plasma cells. histological examination of ... | 1976 | 4220 |
| lipolysis in human adipose tissue (author's transl). | lipolytic activity was measured under different conditions in isolated fat cells and homogenates of human adipose tissue of the greater omentum. it was demonstrated that lipolysis took place in isolated fat cells at an optimum ph of 7.4 and was markedly stimulated by noradrenaline, but not by blood serum. by contrast lipolysis was significantly stimulated by blood serum, but not by noradrenaline, in homogenates of adipose tissue. serum-stimulated lipolysis exhibited optimum activity at ph 8 and ... | 1976 | 3890 |