| leishmania major: the suitability of east african nonhuman primates as animal models for cutaneous leishmaniasis. | the susceptibility of four species of east african nonhuman primates to experimental infection with leishmania major was investigated. four syke's monkeys (cercopithecus mitis), two vervet monkeys (cercopithecus aethiops), two baboons (papio cynocephalus), and two brown bushbabies (galago garnettii) were each inoculated intradermally on the left eyelid, left ear, and nose with 0.1 ml of medium containing 1 x 10(7) promastigotes of a characterized l. major strain. all the nonhuman primates except ... | 1987 | 3678449 |
| intermolecular homologous recombination between transfected sequences in mammalian cells is primarily nonconservative. | intermolecular recombination in mammalian cells was studied by coinfecting african green monkey cells in culture with two shuttle vector plasmids, each carrying an incomplete but overlapping portion of the gene for neomycin resistance. the region of homology between the two plasmids was about 0.6 kilobases. recombination between the homology regions could reconstruct the neomycin resistance gene, which was monitored by analysis of progeny plasmids in bacteria. the individual plasmids carried add ... | 1987 | 3683393 |
| seasonal variation of serum lipids in the vervet monkey. | seasonal variation of serum lipids has been observed in man, primates and other species. we are presenting data on serum lipid variation in vervet monkeys (cercopithecus aethiops). the monkeys (54 males, 33 females) were fed control diets (commercial pellets, fruit, vegetables) and data were pooled and grouped by season. data having been obtained in south africa, the seasons were: summer (december, january, february); fall (march, april, may); winter (june, july, august); and spring (september, ... | 1987 | 3689478 |
| evaluation of the hypolipidemic activity of xenalipin in experimental animals. | xenalipin (4'-trifluoromethyl-2-biphenylcarboxylic acid) is a chemically novel compound which has been found to be an effective hypolipidemic agent in two animal species. significant reductions in serum cholesterol and triglycerides were observed in cholesterol-cholic acid-fed rats following oral doses of 10-30 mg/kg/day. xenalipin was considerably more potent than clofibrate, nicotinic acid, and cholestyramine in the same model. lipoprotein analysis showed that xenalipin reduced cholesterol and ... | 1987 | 3689493 |
| congenital malformations and twinning in a breeding colony of old world monkeys. | clinical and necropsy records of malformations in old world monkeys were compiled. the numbers of malformations and birth incidence rate for each species were: rhesus macaque (macaca mulatta) 3 (1.02%); cynomolgus macaque (macaca fascicularis) 11 (1.62%); stumptail macaque (macaca arctoides) 3 (1.55%); african green monkey (cercopithecus aethiops) 4 (1.5%). there was one pair of rhesus twins (twinning rate: 0.34%). cardiovascular and central nervous system lesions accounted for 55% of all malfor ... | 1987 | 3695398 |
| craniosynostosis in two african green monkeys. | multiple craniosynostoses were found in two infant african green monkeys. the bones of the calvariums were overlapped and fused at all the cranial sutures with resulting pressure necrosis of the cerebral cortices. the cause of the craniosynostoses is unknown, but the two infants were born within 15 days of each other and had the same father, suggesting either exposure to a teratogen or a genetic etiology. the overlapping of the cranial bones is unlike craniosynostosis in humans. | 1987 | 3695399 |
| encephalomyocarditis virus infection in african green and squirrel monkeys: comparison of pathologic effects. | this study compared the pathological effects of experimental encephalomyocarditis virus infection in african green monkeys (cercopithecus aethiops) and squirrel monkeys (saimiri sciureus). african green monkeys died within 5 days post-inoculation and significant changes were limited to moderate to severe multifocal nonsuppurative necrotizing myocarditis. squirrel monkeys were less severely affected and died or were euthanized 4 to 41 days post-inoculation. myocardial and central nervous system c ... | 1987 | 3695400 |
| conjugates of biogenic amines in ventriculocisternal perfusates of conscious african green monkeys. | to determine the importance of conjugation relative to alternate metabolic pathways for the inactivation of catecholamines and serotonin, free and conjugated norepinephrine, dopamine and serotonin, together with the acid metabolites homovanillic acid and 5-hydroxyindoleacetic acid, were measured in ventriculocisternal perfusions in awake african green monkeys. measurements were made of endogenous transmitters and their metabolites using high performance liquid chromatography with electrochemical ... | 1986 | 3708364 |
| synthesis and stability of nuclear matrix proteins in resting and serum-stimulated swiss 3t3 cells. | the major [35s]methionine-radiolabeled nuclear matrix proteins of mouse 3t3 cells were isolated, and most of these were found to be similar in molecular weight, charge, and protease fingerprint to the nuclear matrix proteins of african green monkey kidney cells, which are found tightly bound to simian virus 40 chromosomes. these nuclear matrix proteins were found to be synthesized in quiescent and serum-stimulated cells and to be turned over slowly during pulse-chase experiments. in contrast, a ... | 1986 | 3711149 |
| an improved hemagglutination test for study of canine parvovirus. | optimal conditions for hemagglutination (ha) by canine parvovirus (cpv) strains were investigated using several buffers. porcine erythrocytes often agglutinated spontaneously in phosphate-buffered salt solution, isotonic saline solution or barbitone-complement-fixation buffer. results were reproducible when borate-buffered saline (bbs) was used as the diluent for antigen, and "virus adjusting diluent" (vad), containing 0.15 m nacl and 0.3 m phosphate was used as the diluent for erythrocytes. hig ... | 1986 | 3727364 |
| activity of 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodouracil against simian varicella virus infections in african green monkeys. | the fluorinated pyrimidines 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodouracil (fiau) and 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau) are highly effective inhibitors of herpesvirus infections in vitro and in vivo. this report is concerned with an evaluation of their activities in african green monkeys (cercopithecus aethiops) infected with simian varicella virus, a herpesvirus closely related to human varicella-zoster virus. oral or intravenous administration of ... | 1986 | 3729332 |
| cytoskeletal changes during adhesion and release: a comparison of human and nonhuman primate platelets. | the organization of cytoskeletal proteins in whole-mount adherent platelets from african green monkeys and normal human volunteers has been studied by sem, high vacuum electron microscopy (hvem) and conventional (120 kv) electron microscopy. we describe three distinct organizational zones, the central matrix, the trabecular zone and the peripheral web in spread platelets from both sources. the central matrix is an ill-defined superstructure of 80-100 a filaments of short length which enshrouded ... | 1986 | 3738417 |
| amitotic neuroblastoma cells used for neural implants in monkeys. | the potential utility of cultured neuroblastoma cells as donor tissue for neutral implants into the mammalian brain has been examined. cells from a human neuroblastoma cell line, imr-32, were labeled with [3h]thymidine and chemically rendered amitotic. these differentiated imr-32 cells were grafted into the hippocampi of five adult african green monkeys, and graft survival was evaluated for up to 270 days after transplantation. autoradiographically labeled grafted cells were identified in four a ... | 1986 | 3749886 |
| metabolism of t-2 mycotoxin by cultured cells. | t-2 mycotoxin is a small (i.e. mol. wt 466), non-protein toxin. we studied its metabolism in chinese hamster ovary (cho) cells, african green monkey kidney (vero) cells, human fibroblasts and mouse connective tissue cells (l-929). confluent cells were exposed to [3h]-t-2(0.01 micrograms/ml) for 1 hr at 37 degrees c. the toxin was removed, cells rinsed, and unlabeled culture media added for 4 hr (37 degrees c). cell monolayers were extracted and media and cell extracts were spotted on thin-layer ... | 1986 | 3750347 |
| expression and cell type--specific processing of human preproenkephalin with a vaccinia recombinant. | the posttranslational maturation of a complex precursor polyprotein, human proenkephalin, was assessed by infection of a wide spectrum of cell types with a recombinant vaccinia virus that expressed human proenkephalin. the infected cells rapidly produced both cellular and secreted met-enkephalin immunoreactivity. although each cell line could secrete intact proenkephalin, only a mouse pituitary line was capable of processing proenkephalin to mature enkephalin peptides. the quantity of intact pro ... | 1986 | 3754979 |
| a species comparison of low density lipoprotein heterogeneity in nonhuman primates fed atherogenic diets. | six male cynomolgus monkeys and five male african green monkeys were fed dietary cholesterol to induce hypercholesterolemia. the two groups studied had equivalent total plasma cholesterol concentrations. low density lipoproteins (ldl) were isolated from whole plasma by ultracentrifugation and separated from other lipoprotein classes by agarose column chromatography. ldl were further subfractionated by density gradient ultracentrifugation in a vti-50 vertical rotor. the material within five densi ... | 1986 | 3760711 |
| reproduction in the vervet monkey (cercopithecus aethiops): endometrial oestrogen and progestin receptor dynamics during normal and prolonged menstrual cycles. | sixteen individually caged adult female vervet monkeys (cercopithecus aethiops), whose reproductive parameters had been studied for 5 years, were hysterectomized/ovariectomized during three reproductive states; i.e. the late follicular (15.4 +/- 4.7 (s.d.) days) and luteal (27.8 +/- 4.7 days) phases of the normal cycle (20-50 days), and during prolonged intermenstrual intervals (pii; 99.0 +/- 2.5 days since the previous menses). these latter animals showed characteristics of both follicular and ... | 1986 | 3760741 |
| effects of chlorine dioxide on thyroid function in the african green monkey and the rat. | in a previous study from this laboratory, chlorine dioxide (clo2) treated drinking water depressed thyroxine (t4) levels in the african green monkey. the present study again demonstrated a decrease in t4 levels in the same species after 4 wk of oral exposure. however, after 8 wk of treatment t4 levels rebounded to above pretreatment levels, coinciding with an increase in thyroid radioiodide uptake. this t4 rebound phenomenon and increased iodide uptake may be due to a compensatory endocrinologic ... | 1986 | 3761383 |
| imprints of virus infection: can paramyxoviruses permanently modify triacylglycerol metabolism? | we have examined whether the passage of a paramyxovirus in a cell (bgm, african green monkey kidney) or animal (swiss mouse) can permanently modify its metabolism. in an in vitro model in which cells had been cured of a measles virus persistent infection, the cells retained the modifications affecting lipid metabolism and composition induced during the infection. in a canine distemper virus mouse model, the same virus-induced modifications were observed in mice after the virus had been eliminate ... | 1986 | 3762334 |
| immunocytochemical localization of s-100 protein in stellate cells (folliculo-stellate cells) of the adenohypophysis in the monkeys macaca irus and cercopithecus aethiops. | with the use of an antibody against bovine s-100 protein, it was possible to reveal a characteristic cell type in the pars distalis and the pars tuberalis of the monkey macaca irus. in the adenohypophysis of cercopithecus aethiops, labeled cells were present in the pars distalis, pars tuberalis, and pars intermedia. these cells, so-called folliculo-stellate cells, were found in all pituitaries studied. surprisingly, an antibody against human s-100 protein did not label the stellate cells of the ... | 1986 | 3779805 |
| early placentation in the african green monkey (cercopithecus aethiops). | placentation in the green monkey (cercopithecus aethiops) from gestational ages of 18-30 days was studied by light and electron microscopy. the placentae show many features in common with that of the rhesus monkey and other related macaques. implantation is superficial and bidiscoid, and the placenta is classified as villous and hemomonochorial. epithelial plaque formation and a moderate stromal reaction occur. plaque cells are large, with massive deposits of glycogen and marginal pleomorphic de ... | 1986 | 3788465 |
| differential toxicity of cis- and trans-diamminedichloroplatinum(ii) toward mammalian cells: lack of influence of any difference in the rates of loss of their dna-bound adducts. | dna-bound adducts formed by cis- or trans-diamminedichloroplatinum(ii) (cis-ddp or trans-ddp, respectively) have very different effects on dna synthesis and cell cycle progression in chinese hamster cells. when the loss of platinum from the dna of cells treated with pulsed doses of these isomers was corrected for the different effects they produced on dna replication and cell cycle progression, then adducts formed by either agent were lost slowly from dna and at comparable rates. the kinetics of ... | 1987 | 3791217 |
| molybdate and the molecular properties of the vervet monkey estrogen receptor. | the vervet monkey (cercopithecus aethiops pygerythrus) uterine estrogen receptor was partially characterised. the effect of the molybdate oxyanion on various molecular properties of the receptor was investigated. molybdate appeared to affect the subunit structure and apparent heterogeneity of the receptor. anion exchange chromatography of uterine cytosols yielded two ligand binding subunits in a 1:1 ratio in the absence of sodium molybdate, while only a single labelled complex could be demonstra ... | 1986 | 3795954 |
| studies on the mechanism of entry of vaccinia virus in animal cells. | in order to study the mechanism of entry of vaccinia virus into cells the fate of virion associated polypeptides was investigated during infection of african green monkey kidney (bsc-40) cells with 35 s-methionine labelled virus. approximately 12-15 percent of the virion polypeptides were degraded to acid-soluble products by 3 hours post-infection. proteolysis was inhibited (50 percent) by methylamine, suggesting a lysosomal site of degradation. neither methylamine or chloroquine inhibited virus ... | 1987 | 3800657 |
| spontaneous malignant lymphoma in an african green monkey naturally infected with simian t-lymphotropic virus (stlv). | an african green monkey naturally infected with simian t-lymphotropic virus (stlv) developed spontaneous malignant lymphoma of diffuse pleomorphic type. the clinical, hematological and histopathological characteristics were very similar to those of human adult t-cell leukemia. | 1986 | 3806651 |
| the suitability of east african primates as animal models of visceral leishmaniasis. | the susceptibility of four east african primate species to experimental infection with leishmania donovani was investigated. vervet monkeys (cercopithecus aethiops), sykes monkeys (c. mitis) and baboons (papio cynocephalus) all supported low grade infections for periods ranging between four and eight months and subsequently showed evidence of self-cure. greater bushbabies (galago crassicaudatus) remained completely refractory throughout the course of the experiment. the significance of hepatic h ... | 1986 | 3810791 |
| alteration of high density lipoprotein subfractions of nonhuman primates fed fish-oil diets. selective lowering of hdl subfractions of intermediate size and density. | two groups of adult male african green grivet monkeys (12 animals in each group) were fed atherogenic diets containing 40% of total calories as fat with half of the fat calories derived from lard or menhaden oil and 0.8 mg cholesterol/kcal for a period of 10 months to ascertain the effect of such diets on lipoprotein concentrations, composition, and distribution. after 8 months on this diet, the animals' lipoproteins were isolated from their plasma by ultracentrifugation and agarose column chrom ... | 1987 | 3813977 |
| leptospires in vervet monkeys (cercopithecus aethiops sabaeus) on barbados. | agglutinins to leptospira were found at titers of greater than or equal to 1:100 in 150 of 501 (29.9%) vervet monkeys (cercopithecus aethiops sabaeus) bled within 1 mo of capture in barbados. including a further 34 of 145 bled within 1 yr of capture, the seropositivity prevalence was 28.5%. a further 35 monkeys (5.4%) had traces of agglutinins or gave titers of 1:50. the proportion of seropositive adults (41.5%) was more than twice that of seropositive immature monkeys (17.6%). among adults, 49. ... | 1987 | 3820429 |
| [nucleolus-forming regions in the chromosomes in continuous cell cultures]. | localization of nucleoli-forming (nf) areas in chromosomes of continuous cell cultures of african green monkey kidney vero, cv-1, gmk, bgm, bs-c-1. macaca rhesus kidney llc-mk2, dog kidney mdck, pig kidney spev, pk-15 was studied. the nf-areas in chromosomes of african green monkey, m. rhesus, and pig cell cultures were shown to be localized in sites of secondary chromosomal stangulation, and there was also a definite correlation between the intensity of staining of nf-areas and sizes of the zon ... | 1986 | 3825094 |
| [ramt cell line as a substrate for the propagation of vaccinal viral strains]. | properties of the pamt cell line which are deficient in hypoxanthine guanyl phosphoribosiltransferase were studied. the pamt cell line derived from african green monkey kidney tissue may be identified by selective nutrient media, contains no contaminants, is not tumorigenic, is susceptible to a wide range of viruses. titres of poliomyelitis, tick-borne encephalitis, and carnivore plague viruses were similar in cell cultures grown in the medium with 10% serum and in those adapted to growth in the ... | 1986 | 3825095 |
| some morphological characteristics of large sulci situated on the outer side of grivet monkey cerebral hemisphere. | | 1986 | 3825586 |
| genital signalling and the coexistence of male vervet monkeys (cercopithecus aethiops pygerythrus). | to ascertain the social significance of male genital colouration two troops of vervets, one free-ranging and one caged, were observed. it was found that genital signals were associated with intermale agonism. it is suggested that this communicatory system regulates the behaviour of competitors and may thereby have facilitated the evolution of a multimale social system. | 1985 | 3833622 |
| morphological changes in cho and vero cells treated with t-2 mycotoxin. correlation with inhibition of protein synthesis. | exposure of chinese hamster ovary and african green monkey kidney cells to t-2 mycotoxin resulted in several morphological changes which were related to inhibition of protein synthesis, the basic in vitro mechanism of action of the toxin. these changes, which occurred in both cell types, included disassociation of polysomes and mitochondrial cristae alterations. in addition, cho cells displayed membrane bleb formations similar to those found in cho cells after exposure to established inhibitors ... | 1985 | 3836022 |
| nucleotide sequence analysis and enhancer function of long terminal repeats associated with an endogenous african green monkey retroviral dna. | the nucleotide sequence and enhancer activity of the long terminal repeats (ltrs) associated with a cloned endogenous african green monkey (agm) retroviral dna designated as lambda-agm-1 was studied. a unique feature of the endogenous agm proviral ltrs was the presence of multiple copies of two types of directly repeating units in the u3 region: 16 8-base-pair (bp) repeats were present in the 5' ltr and 12 were present in the 3' ltr which were bound by a 6-bp perfect direct repeat; tandem duplic ... | 1985 | 3861934 |
| protein synthesis by mammalian cells treated with c-3-modified analogs of the 12,13-epoxytrichothecenes t-2 and t-2 tetraol. | modification at the c-3 position of the trichothecenes t-2 and t-2 tetraol affected their ability to inhibit protein synthesis in african green monkey kidney (vero) and mouse erythroleukemia cells. replacement of the 3-hydroxyl of t-2 with hydrogen caused a 24-fold decrease in activity, whereas acetylation resulted in a 500-to 1,000-fold decrease. protection of the 3-hydroxyl with a tetrahydropyranyl moiety gave an analog that was 37-fold more inhibitory to vero than to mouse erythroleukemia cel ... | 1985 | 3866515 |
| recombination and deletion of sequences in shuttle vector plasmids in mammalian cells. | shuttle vector plasmids were constructed with directly repeated sequences flanking a marker gene. african green monkey kidney (agmk) cells were infected with the constructions, and after a period of replication, the progeny plasmids were recovered and introduced into bacteria. those colonies with plasmids that had lost the marker gene were identified, and the individual plasmids were purified and characterized by restriction enzyme digestion. recombination between the repeated elements generated ... | 1985 | 3869955 |
| a growth regulatory factor that can both inhibit and stimulate growth. | a growth inhibitor that is produced by bsc-1 cells (african green monkey kidney epithelial cells) has been isolated from conditioned medium. it has been purified by gel chromatography and high performance liquid chromatography. it appears to be a protein with a relative molecular mass (mr) of 24 000. it is extremely active as a growth inhibitor with some cells, but not with others. approximately 50% inhibition of thymidine incorporation is observed with ccl64 cells at 0.05 ng/ml and with bsc-1 c ... | 1985 | 3878272 |
| circadian rhythm of hmg-coa reductase and insulin in african green monkeys. | hmg-coa reductase in hepatic microsomes and serum insulin display circadian rhythm in two strains of cercopethicus aethiops. grivets develop higher levels of serum cholesterol than vervets fed cholesterol. males (n = 20/gp) were adapted to a light cycle (7:30 a.m.-8:30 p.m.) for 60 days and fed a non-cholesterol diet at 8:30 a.m. and 2:00 p.m. vervet acrophase, reductase activity was synchronized to serum insulin units with a specific activity of 1480 pmol/min/mg protein changing by 7.5-fold fro ... | 1985 | 3888223 |
| the effects of ketamine anesthesia on glucose clearance in african green monkeys. | ketamine hydrochloride's effect on glucose clearance-insulin secretion during intravenous glucose tolerance testing was studied in five african green monkeys. animals were tested with ketamine anesthesia and then had indwelling cannulas implanted and were retested both in the presence and absence of ketamine anesthesia. serum glucose and insulin concentrations were determined. there were no significant differences in the glucose clearance rate (k value), basal glucose and insulin concentrations, ... | 1985 | 3889340 |
| the pulpal response to dilute citric acid smear removers. | the aim of this study was to determine the pulpal response to three dilute citric acid smear removers. forty-eight vervet monkeys (cercopithecus aethiops pygerythrus), in three groups of sixteen animals provided 384 tooth specimens for the histological evaluation of the pulpal response at 3, 31, and 59 days post-operatively. labial class v cavities were prepared in the maxillary and mandibular incisor teeth. the pulpal responses elicited by 1% aqueous citric acid, 1% citric acid in 30% ethanol a ... | 1985 | 3891932 |
| cytotoxicity testing of a dentin bonding system. | the cytotoxicity of a newly developed dentin bonding system was examined in african green monkey kidney cells and human embryonic lung cells. a tissue culture agar overlay procedure, a longitudinal study, and a cell replication assay were employed to evaluate cytotoxicity. several of the components were discovered to be cytotoxic when tested individually in vitro. however, when used in the prescribed manner, little or no cytotoxic effect was elicited. the results of this study support further te ... | 1985 | 3892412 |
| hormonal responses to gonadotrophin releasing hormone during testicular development in male african green monkeys. | reproductive development in male african green monkeys was characterized by evaluating both luteinizing hormone (lh) and testosterone (t) before and after gonadotrophin releasing hormone (gnrh) stimulation in relation to the physical maturation of the testis. there were lh responses to gnrh at all ages studied, but the failure of some animals to respond at earlier ages suggested developmental changes in the responsiveness of the pituitary. the t secretion developed progressively but did not reac ... | 1985 | 3908686 |
| [gallbladder measurements in the green monkey (cercopithecus aethiops) and their dependence on liver and body weight]. | | 1985 | 3917106 |
| experimental leprosy in three species of monkeys. | eleven mangabey monkeys inoculated with mycobacterium leprae developed lepromatous-type leprosy. nine of the mangabeys were inoculated with m. leprae isolated from a mangabey with naturally acquired lepromatous leprosy. immune function was depressed in some of these animals after dissemination of the disease. two mangabeys developed lepromatous leprosy after inoculation with human m. leprae passaged in an armadillo. three rhesus and three african green monkeys inoculated with mangabey-derived m. ... | 1985 | 3917577 |
| the effect of apicoectomy before replantation on periodontal and pulpal healing in teeth in monkeys. | the effect of resection of the apical part of the root before replantation upon periodontal and pulpal healing was studied in 7 green vervet monkeys (cercopithecus aethiops) using teeth with incomplete and complete root formation. maxillary central incisors, mandibular lateral incisors and first and second mandibular molars were used in this experiment. on one side, the tooth was extracted and replanted with an intact root while the contralateral tooth had 2 mm of the apex resected for the purpo ... | 1985 | 3920162 |
| the heat shock consensus sequence is not sufficient for hsp70 gene expression in drosophila melanogaster. | a hybrid gene in which the expression of an escherichia coli beta-galactosidase gene was placed under the control of a drosophila melanogaster 70,000-dalton heat shock protein (hsp70) gene promoter was constructed. mutant derivatives of this hybrid gene which contained promoter sequences of different lengths were prepared, and their heat-induced expression was examined in d. melanogaster and cos-1 (african green monkey kidney) cells. mutants with 5' nontranscribed sequences of at least 90 and up ... | 1985 | 3920509 |
| recombinant huifn-gamma prevents herpes simplex keratitis in african green monkeys: demonstration of synergism with recombinant huifn-alpha 2. | human interferon gamma (huifn-gamma) was found to prevent herpes-simplex virus (hsv 1) induced keratitis in monkey eyes, when administered topically at concentrations of greater than or equal to 3 x 10(5) reference units/ml. protective efficacy demonstrated with lower concentrations of huifn-gamma in combination with low titers of huifn-alpha provided evidence of synergistic interferon activity in vivo. tolerance problems observed in eyes affected by virus inoculation seem to be attributable to ... | 1985 | 3929044 |
| x-ray-induced base sequence damage in primate alphoid dna. | radiation-induced single-strand breaks were found throughout the 172 bp repeat units of african green monkey component alpha dna. two kinds of 3'-ends of 5'-32p-labeled restriction fragments were found, as previously described by others. after irradiation in vitro, the yield of single-strand breaks was 4 x 10(-5) breaks/nucleotide/gy, as determined by analyses in dna sequencing type gels. protection from x-ray damage was found when the dna received 150 gy in the presence of 2-mercaptoethanol. th ... | 1986 | 3952274 |
| movement of naegleria fowleri stimulated by mammalian cells in vitro. | naegleria fowleri amebae, but not those of n. australiensis, n. gruberi, or n. lovaniensis, demonstrated enhanced motility when placed in proximity to mammalian cells. amebae of nonpathogenic species of naegleria, however, were more motile in cell culture medium than the amebae of n. fowleri. the locomotory response of highly pathogenic mouse-passaged n. fowleri amebae to nerve cells was greater than axenically cultured amebae. the enhanced mobility elicited by whole nerve cells or disrupted ner ... | 1986 | 3959003 |
| micropuncture study of the superficial nephron of cercopithecus aethiops. | clearance and micropuncture experiments were performed on male cercopithecus monkeys weighing between 2 and 4 kg to evaluate the function of the superficial nephron. the mean glomerular filtration rate was 4.47 +/- 0.32 ml/min. the fraction of filtered sodium, potassium, calcium, magnesium, and phosphate remaining at the end of the accessible proximal tubule were 54 +/- 3, 64 +/- 4, 70 +/- 3, 79 +/- 5, and 39 +/- 5%, respectively. the concentration of magnesium and calcium in the tubule fluid ro ... | 1986 | 3961264 |
| scanning electron microscopic examination of the secondary constriction of vero cells. | vero cells (african green monkey kidney in origin) were prepared by the conventional air-drying method and then processed for sem by a modification of the conductive method based on thiocarbohydrazide-osmium binding. under sem, not only metaphase chromosomes but also resting nuclei showed distinct fibres 30 nm in diameter. a few such fibres were found to run across the secondary constriction of the nor-carrying chromosome. | 1985 | 3979451 |
| steroid hormone influences on the mating behavior of vervet monkeys (cercopithecus aethiops). | eight ovariectomized female vervet monkeys (cercopithecus aethiops) were tested in heterosexual and isosexual pairs, under estradiol and testosterone treatments. pairs were tested under two different experimental situations in which the male was either restrained or left free. there were only small behavioral changes across the treatments, involving mainly male responses. erection time, frequency of intromission, ejaculation, and olfactory investigation increased during estradiol treatment where ... | 1985 | 3979968 |
| cloning of nascent monkey dna synthesized early in the cell cycle. | to study the structure and complexity of animal cell replication origins, we have isolated and cloned nascent dna from the onset of s phase as follows: african green monkey kidney cells arrested in g1 phase were serum stimulated in the presence of the dna replication inhibitor aphidicolin. after 18 h, the drug was removed, and dna synthesis was allowed to proceed in vivo for 1 min. nuclei were then prepared, and dna synthesis was briefly continued in the presence of hg-dctp. the mercury-labeled ... | 1985 | 3990692 |
| effects of ketamine anaesthesia, stress and repeated bleeding on the haematology of vervet monkeys. | haematology values are presented for the vervet monkey (cercopithecus aethiops), and the relative effects of high dose ketamine anaesthesia, stress of capture and repeated bleedings assessed. anaesthesia resulted in decreased wbc and rbc values, attributed to depression of cardiovascular function. these effects were the reverse of those of alarm and strenuous exercise (leukocytosis and polycythaemia) during capture. stress resulted in relatively high white and low red blood cell counts. opposing ... | 1985 | 3999687 |
| cytotoxic effects of culture supernatants of oral bacteria and various organic acids on vero cells. | dilute culture filtrates of the three asaccharolytic black-pigmented bacteroides species as well as b. macacae, fusobacterium nucleatum, and animal strains resembling b. gingivalis were cytotoxic for the african green monkey kidney cell line (vero cells). butyric or propionic acid, major metabolic products of these bacteria, seem to be responsible for the characteristic morphological changes observed. among the organic acids studied, only butyric, propionic, and valeric acids exhibited cytotoxic ... | 1985 | 4005713 |
| interferon inhibits marker rescue of vaccinia virus. | in this investigation we have examined the effect of human interferon (ifn) type alpha on the ability of vaccinia virus to recombine within infected african green monkey kidney cells (bsc-40). we measured by marker rescue, the extent of insertion of cloned 5-kb hind iii-j restriction fragment of wild-type vaccinia dna into the genome of temperature-sensitive mutants. we showed that ifn at doses of 100-1000 u/ml inhibited the replication of vesicular stomatitis virus (vsv) and polio viruses but n ... | 1985 | 4009001 |
| effects of the degree of saturation of dietary fat on the hepatic production of lipoproteins in the african green monkey. | the cholesteryl ester content of plasma low density lipoproteins (ldl) in monkeys has previously been shown to be related to the rate of hepatic cholesterol secretion and cholesteryl ester content of newly secreted lipoproteins in the isolated perfused liver. in the present studies, african green monkeys were fed diets containing cholesterol and 40% of calories as either butter or safflower oil in order to determine the effects of saturated versus polyunsaturated dietary fat on hepatic lipoprote ... | 1985 | 4009059 |
| discriminative stimulus properties of lysergic acid diethylamide in the monkey. | four monkeys (cercopithecus aethiops) were trained to discriminate 0.06 mg/kg of lysergic acid diethylamide (lsd) from saline in a two-key task in which correct responding was reinforced with food under a fixed ratio 32 schedule. the ed50 of lsd was 0.011 mg/kg. the nonhallucinogenic ergot, lisuride, and the hallucinogen, 5-methoxy-n,n-dimethyltryptamine, substituted completely for lsd (ed50 values were 0.0098 and 0.45 mg/kg, respectively). mescaline (1-40 mg/kg), d-amphetamine (0.1-0.625 mg/kg) ... | 1985 | 4009503 |
| cell proliferation-associated expression of a recently evolved isozyme of triosephosphate isomerase. | an electrophoretically unique, thermolabile isozyme of triosephosphate isomerase (tpi; ec 5.3.1.1) accounts for 10-30% of the enzymatic activity in a range of mitotically active human cells and tissues. this type 2 form (subunit) of human tpi appears in two isozymes, an anodally migrating, relative to the constitutive tpi-1/1 homodimer, tpi-2/2 homodimer and the tpi-1/2 heterodimer with an intermediate mobility. human cell types expressing the induced isozyme, which is the product of the same st ... | 1985 | 4015618 |
| the organization of two related subfamilies of a human tandemly repeated dna is chromosome specific. | several clones containing clusters of repetitive elements were isolated from a human chromosome 22 specific library. an ecori-xhoi fragment of 860bp was subcloned and was shown to belong to a family of tandemly repeated dna linked to the y-specific 3.4 kb haeiii band. this probe hybridizes to several sets of sequences or subfamilies. the most abundant subfamily is a 1.8kb long sequence containing one ecorv site, and in most repeats, one avaii and one kpni site. using human-rodent somatic cell hy ... | 1985 | 4018797 |
| trypanosomal cardiac valvulitis in vervet monkeys. | quarantined vervet monkeys (cercopithecus aethiops) were infected with trypanosoma brucei (10(4) parasites/animal) in a tsetse free area. thirteen monkeys (11 infected with t.b. rhodesiense and 2 with t.b. brucei) were studied. animals became parasitaemic within one week after infection. the infection time lasted between 21 and 129 days; in 8 monkeys it was between 50 and 70 days. macroscopically massive pericarditis was observed in one, pericardial effusion in one, small apical aneurysms in thr ... | 1985 | 4023556 |
| acute upper genital-tract disease in female monkeys provoked experimentally by mycoplasma genitalium. | the oviducts of two grivet monkeys and three marmosets, all sexually mature animals, were inoculated with mycoplasma genitalium at laparotomy. the mycoplasma was not recovered from the grivet monkeys, nor from the oviducts of the marmosets although it was isolated intermittently from the vagina of two of the latter animals up to 4-6 weeks after inoculation. in contrast, all of the animals developed antibody to m. genitalium measured by a micro-immunofluorescence technique. it developed rapidly i ... | 1985 | 4027175 |
| cytochalasin d inhibits the progression from the go to s phase at the mid-prereplicative stage in gc-7 cells stimulated with serum. | when the growth of serum-arrested gc-7 cells, a clone from african green monkey kidney, was induced by the addition of 10% calf serum, they began to enter s phase after 15-16 h. when stimulated cells were cultured in the presence of 0.6 micrograms/ml of cytochalasin d, the entrance into s phase was inhibited. treatment of cells with cytochalasin d during the period earlier than 8 h or later than 11 h after the serum stimulation showed no or little inhibitory effect on the entrance of cells into ... | 1985 | 4039633 |
| serum testosterone, male dominance, and aggression in captive groups of vervet monkeys (cercopithecus aethiops sabaeus). | the relationship of serum testosterone concentration to male dominance rank and frequency of aggression was investigated in stable vervet monkey social groups, each containing two or three adult males, several adult females, and their offspring. dominance relationships were determined by noting an animal's success in intermale aggressive encounters. a striking finding was the marked within-subject variation in testosterone concentration: 5- to 10-fold fluctuations were often observed on successi ... | 1985 | 4040114 |
| estrogen and progesterone receptors in the uterus of the vervet monkey. | experimental conditions for the optimal measurement of estrogen (er) and progesterone (pr) receptors in normal vervet monkey (cercopithecus aethiops pygerythrus) uteri are described. the uteri of this primate were found to contain relatively high concentrations of both er and pr. levels of er ranged from 151 to 822 femtomoles per mg protein (mean for group assayed is 327 +/- 165 femtomoles per mg protein). pr assays were performed on the same cytosols and the levels ranged from 444 to 2267 femto ... | 1985 | 4040971 |
| characterization of angiotensin binding in the african green monkey. | the observation that there are differences in the characteristics and distribution of angiotensin receptors in the central nervous system of mammalian species led to the analysis of angiotensin binding in a primate model, the african green monkey. initial studies using [125i]angiotensin ii ([125i]aii) as the radioligand showed binding in peripheral tissues but little binding in the central nervous system. conversely, binding studies using [125i]aiii as the radioligand indicated more central nerv ... | 1985 | 4041782 |
| captive propagation of vervet monkeys (cercopithecus aethiops) in harems. | a small breeding colony of captive vervet monkeys (cercopithecus aethiops), consisting of ten single male harem groups, was established in kenya. vervets were extremely prone to stress after capture and manipulative procedures led to high mortalities. six weeks were required for partial habituation, after which routine handling was not too problematic. however, complete adjustment to captivity took a minimum of one year. establishment of stable breeding groups from adult animals initially proved ... | 1985 | 4046547 |
| local dermatitis with subsequent pigmentation in green monkeys (cercopithecus aethiops). | | 1985 | 4050159 |
| notes on morpho-functional differences between the meissner's corpuscles of man and the green monkey (cercopithecus aethiops l.). | | 1985 | 4054753 |
| mitochondrial and plasma membrane potentials cause unusual accumulation and retention of rhodamine 123 by human breast adenocarcinoma-derived mcf-7 cells. | quantitative studies of mcf-7 cells (derived from human breast adenocarcinoma) and cv-1 cells (from normal african green monkey kidney epithelium), using the permeant cationic compound tetraphenylphosphonium (tpp), in conjunction with fluorescence microscopy using rhodamine 123 (rh123), indicate that the mitochondrial and plasma membrane potentials affect both uptake and retention of these compounds. under conditions that depolarize the plasma membrane, uptake and retention of tpp and rh123, dri ... | 1985 | 4055760 |
| reversibility of the stabilization effect of sodium molybdate on uterine estrogen and progesterone receptors of the vervet monkey. | sodium molybdate affected the stability of vervet monkey (cercopithecus aethiops pygerythrus) uterine estrogen (er) and progesterone (pr) receptors. yields of receptors were invariably higher (20-40%) when cytosols were prepared in the presence of 10mm sodium molybdate. no changes were observed in the binding affinities for the natural ligands as reflected in dissociation constants. receptor-ligand association at 0 degrees c and 20 degrees c was not affected in the presence or absence of molybda ... | 1985 | 4057178 |
| activation of metastatic potential in african green monkey kidney cell lines by prolonged in vitro culture. | studies on the tumorigenicity of vero kidney cells of cercopithecus aethiops monkey origin were extended to various passage levels of bsc-1 aneuploid cells and to low passage cv-1 diploid cells (derived also from c. aethiops monkey kidney). it was found that bsc-1 cells -- like vero cells -- showed increased tumorigenicity with increasing passage level in antithymocyte globulin (atg) treated newborn rats and in nude mice. cells passaged over 250 times in cultures formed invasive adenocarcinomas ... | 1985 | 4066602 |
| [activity of the nucleolus-organizing regions of the chromosomes of normal and transformed spleen cells in the african green monkey]. | the activity of nucleolus organizer regions (nor's) of chromosomes in the african green monkey cells has been studied before and after the transformation. in cells before their transformation, one or two chromosomes entering into association and possessing nors are involved in the formation of one nucleolus. in transformed cells no correlation between the numbers of nucleoli in interphase and those of chromosomes possessing nors was revealed in metaphase plates. it is supposed that in chromosome ... | 1985 | 4071663 |
| behavioral aspects of serotonin-dopamine interaction in the monkey. | the effects of serotonin (5-hydroxytryptamine; 5-ht) antagonists and 5-ht uptake inhibitors on the behavioral response to amphetamine and haloperidol in monkeys (cercopithecus aethiops) were investigated. amphetamine increased locomotor activity and reactivity and induced repetitive movements of head, limbs and trunk, but no oral hyperkinesia. haloperidol induced dystonia and parkinsonism. pretreatment with the 5-ht antagonists cyproheptadine and mianserin increased amphetamine-induced locomotor ... | 1985 | 4085556 |
| variable satellite dna's in the african green monkey cercopithecus aethiops. | | 1974 | 4134868 |
| stereoselective biotransformations of dl-norgestrel and its enantiomers in the african green monkey. | | 1974 | 4150136 |
| relationship of some vibrio antibodies to serum immune globulins in man and in cercopithecus aethiops. | | 1966 | 4161200 |
| [light and electron microscopic studies of the structure and innervation of the ciliary muscle in the human and monkey (cercopithecus aethiops)]. | | 1967 | 4172225 |
| electron optical investigations on the morphological alterations produced by subacute sclerosing panencephalitis (sspe) virus in cercopithecus aethiops (r 6 ca) kidney cells. | | 1973 | 4196869 |
| replication pattern and quinacrine fluorescence of the chromosomes of a cv-1-derived african green monkey cell line. | | 1973 | 4197165 |
| unusual intranuclear tubular structures associated with the maturation of herpesvirus saimiri in monkey kidney cell cultures. | | 1973 | 4198465 |
| production of macrophage migration inhibition factors by virus-infected cell cultures. | macrophage migration inhibitory (mif-like) activity was demonstrated in the supernatant fluids from cultures of african green monkey kidney cells (bgm) infected with mumps virus or newcastle disease virus. we could detect no such activity in noninfected cultures. the virus-induced activity reported here is not due to nonspecific cytotoxic material released by dead or dying cells, and it does not require cell replication for its production. preliminary estimates of molecular weight by sephadex g- ... | 1973 | 4199152 |
| long term infection of diploid african green monkey brain cells by schwarz measles vaccine virus. | | 1973 | 4200695 |
| serial passage of 3 strains of avian infectious bronchitis virus in african green monkey kidney cells (vero). | | 1973 | 4203249 |
| susceptibility and immune response to experimental plague in two species of langurs and in african green (grivet) monkeys. | | 1974 | 4207625 |
| the indirect immunoenzyme test for the detection of antibodies against north american borreliae. i. technic and results with sera of vervet monkeys (cercopithecus aethiops). | | 1974 | 4208886 |
| fate of auricular transplants and repair of auricular gaps in the grivet monkey (cercopithecus aethiops aethiops). | | 1974 | 4211009 |
| [a comparison of the pathogenicity of two strains of schistosoma mansoni in cercopithecus aethiops monkeys in the sudan]. | | 1974 | 4214855 |
| some biochemical characteristics of the salivary gland products of macaca irus and cercopithecus aethiops. | | 1974 | 4215613 |
| restriction endonuclease cleavage maps of animal mitochondrial dnas. | the restriction endonuclease, hindiii, gives rise to three fragments in each of the three mitochondrial dnas isolated from the established mammalian cell lines la9 (mouse), hela (human), and bsc-1 (african green monkey). the restriction endonuclease, ecori, gives rise to three fragments in mitochondrial dna from hela and to two in dnas from la9 and bsc-1. the sizes and the orders of the fragments in the respective genomes have been determined with data obtained from the electron microscope. the ... | 1974 | 4216020 |
| an anatomical study of the pineal body of the vervet monkey (cercopithecus aethiops). | | 1974 | 4216961 |
| a histochemical study of the pineal body of the vervet monkey (cercopithecus aethiops). | | 1974 | 4216962 |
| the presence of capillaria hepatica (bancroft, 1893) travassos, 1915 in a vervet monkey cercopithecus aethiops. | | 1974 | 4217471 |
| single and repeated infections of grivet monkeys with schistosoma mansoni: parasitological and pathological observations over a 31-month period. | groups of 6 to 8 grivet monkeys (ceropithecus aethiops aethiops), each of which had been exposed to 600 cercariae of schistosoma mansoni, were killed 3, 6, and 31 months after exposure. other groups of monkeys were repeatedly challenged with cercariae beginning 4 or 23 months after the initial infection, and were killed 31 months after the first exposure. peripheral blood leukocytes, eosinophiles, and schistosome eggs passed in the feces were determined during the course of the infection. at aut ... | 1974 | 4217568 |
| experimental louse-borne relapsing fever in the grivet monkey, cercopithecus aethiops. i. clinical course. | fifteen grivet monkeys, cercopithecus aethiops, were infected with an ethiopian strain of borrelia recurrentis, the causative agent of louse-borne relapsing fever. an initial spirochetemia occurred in all. inactivity, fever, and leukocytosis accompanied the infections. eight of the monkeys experienced 1 relapse and 1 monkey had 2 relapses. the relapses tended to be less severe than the initial infections. the only deaths, however, occurred in 2 of the 3 monkeys with severe relapses. the clinica ... | 1974 | 4217570 |
| experimental louse-borne relapsing fever in the grivet monkey, cercopithecus aethiops. ii. pathology. | nineteen grivet monkeys, cercopithecus aethiops, were infected with the spirochete of louse-borne relapsing fever, borrelia recurrentis, and killed at various intervals following inoculation: 4 during the initial spirochetemia; 4 during remission; 3 during relapse; 2 that were dying following severe relapses; and 6 during convalescence. the histologic changes of histiocytic myocarditis, multiple microabscesses replacing the nodular white pulp of the spleen, and hepatitis with foci of midzonal ne ... | 1974 | 4217571 |
| experimental louse-borne relapsing fever in the grivet monkey, cercopithecus aethiops. iii. crisis following therapy. | eight grivet monkeys infected with borrelia recurrentis received tetracycline (12.5mg/kg body weight) on the 3rd or 4th day of spirochetemia. leukopenia, fever, hyperpnea, and tachycardia developed within 2 hours as spirochete counts fell to undetectable levels. these events closely simulated the crisis in human louse-borne relapsing fever in both timing and extent. | 1974 | 4217572 |
| baseline karyologic values for primary rhesus and african green monkey cell cultures. | | 1974 | 4218606 |
| 3-hydroxyaflatoxin b1: a new metabolite of in vitro aflatoxin b1 metabolism by vervet monkey (cercopithecus aethiops) liver. | | 1974 | 4218848 |
| values for blood constituents of the african green monkey (cercopithecus aethiops). | | 1967 | 4228551 |