Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| chromosomes in a marsupial (potorous tridactylis) tissue culture. | 1962 | 14004541 | |
| the chromosomes of the tasmanian rat-kangaroo (potorous tridactylis apicalis). | 1964 | 14154338 | |
| a biometric analysis of the chromosomes of the marsupials--macropus major, macropus rufus and potorous tridactylis. | 1965 | 14332557 | |
| inner chromosomal structures in the stomatic metaphase of the rat kangaroo (potorous tridactylis). a light microscopic observation. | 1967 | 5610132 | |
| distribution of chromatids at mitosis. | the distribution of labeled chromatids at the second mitosis after labeling with tritiated thymidine is random in both vicia faba (the broad bean) and potorous tridactylis (the rat kangaroo). this finding is contrary to that predicted by the hypothesis that chromatids containing "grandparent" polynucleotide templates segregate from those containing "parent" templates. | 1967 | 6054166 |
| hemogram of the rat kangaroo, potorous tridactylus. | 1968 | 5689539 | |
| effects of the pyrrolizidine alkaloid heliotrine on cell division and chromosome breakage in cultures of leucocytes from the marsupial potorous tridactylus. | 1968 | 5664136 | |
| ultrastructural studies of radiation-induced chromosome damage. | the fine structure of radiation-induced chromosomal aberrations in potorous tridactylis (rat kangaroo) cells was examined in situ by electron microscopy. the observations on the structure of terminal deletions (acentric fragments), anaphase bridges and "gaps," sidearm bridges, and specialized regions, such as the nucleolus organizer, are discussed in detail. conclusions based on these observations are the following: (a) damage is physically expressed only at anaphase; (b) a gap region is compose ... | 1969 | 4895598 |
| the rat kangaroo (potorous tridactylus). handling and husbandry practices in a research facility. | 1969 | 4237319 | |
| studies on marsupial proteins. vi. evolutionary changes of beta-globins of the macropodidae and the amino acid sequence of beta-globin from potorous tridactylus. | 1971 | 5005884 | |
| differences in radiosensitivity of the chromosomes of rat kangaroo (potorous tridactylis) cells in vitro. | 1971 | 5165148 | |
| quinacrine fluorescent patterns of the chromosomes in cell lines of the rat-kangaroo (potorous tridactylus apicalis). | 1971 | 5166377 | |
| laser microirradiation of the nucleolar organizer in cells of the rat kangaroo (potorous tridactylis). reduction of nucleolar number and production of micronucleoli. | 1972 | 4674695 | |
| time of duplication of ribosomal rna cistrons in a cell line of potorous tridactylis (rat kangaroo). | 1972 | 4673370 | |
| the origin, course and termination of the corticospinal tracts of the tasmanian potoroo (potorous apicalis). | 1972 | 5039183 | |
| an anomalous effect of hydroxyurea and visible light on nucleic acids of a photoreactivating mammalian cell potorous tridactylis. | 1972 | 4536933 | |
| the management of a small colony of the marsupial potorous tridactylus, and a record of its breeding in captivity. | 1972 | 4673209 | |
| locomotion in the potoroo. | 1974 | 4819593 | |
| the distribution of x-ray induced chromosomal abnormalities in rat kangaroo (potorous tridactylis) cells in vitro. | 1974 | 4134768 | |
| blood chemistry of the rat kangaroo, potorous tridactylus. | 1974 | 4365344 | |
| dna repair in potorous tridactylus. | the dna synthesized shortly after ultraviolet (uv) irradiation of potorous tridactylis (ptk) cells sediments more slowly in alkali than that made by nonirradiated cells. the size of the single-strand segments is approximately equal to the average distance between 1 or 2 cyclobutyl pyrimidine dimers in the parental dna. these data support the notion that dimers are the photoproducts which interrupt normal dna replication. upon incubation of irradiated cells the small segments are enlarged to form ... | 1974 | 4473053 |
| corticobulbar fibres in the north american opossum (didelphis marsupialis virginiana) with notes on the tasmanian brust-tailed possum (trichosurus vulpecula) and other marsupials. | corticobulbar projections have been studied in the american opossum by both degeneration and autoradiographic methods and, for the most part, the results confirm our earlier observations (martin & west, 1967; martin, 1968). however, we have obtained evidence for certain connexions not previously described and have delineated the origin(s) of several connexions more precisely by paying particular attention to the degeneration present at thalamic levels in all cases and by the use of autoradiograp ... | 1975 | 1213949 |
| ultrastructure of the prophase kinetochore in cultured cells of rat-kangaroo (potorous tridactylis). | 1975 | 1140998 | |
| ultrastructure of the prophase kinetochore in cultured cells of rat-kangaroo (potorous tridactylis). | 1975 | 1137315 | |
| klossiella spp. in the kidneys of peramelid, petaurid, and macropodid marsupials. | klossiella spp. were found in kidneys of individuals from six genera in two of the three superfamilies of australian marsupials. klossiella quimrensis sp.n. is described in the bandicoots, isoodon obesulus and perameles gunnii (peramelidae). k. convolutor sp.n. is described in the ringtail possum pseudocheirus peregrinus (petauridae). three macropodid genera were infected. k.serendipensis sp.n. is described in the swamp wallaby, wallabia bicolor, and k. callitris sp.n. is described in the wester ... | 1975 | 807049 |
| survival-related dna repair phenomena in cultured rat-kangaroo cells. | cultured cells (line ptk-2) from the marsupial mammal rat-kangaroo, or potoroo (potorous tridactylis), which photoreactivate (pr) both uv-induced dimers and lethality, excise few dimers, and are only slightly sensitized by post-uv exposure to caffeine, were subjected to caffeine and hydroxyurea (hu) treatments during the 30-min pr period. it was found that neither caffeine nor hu inhibited pr of lethality as measured by colony-forming ability. further, the cells exhibited no photoprotective prop ... | 1976 | 14997603 |
| oxygen consumption and nitrogen metabolism in the potoroo, potorous tridactylus. | 1976 | 9227 | |
| the absence of centrioles from spindle poles of rat kangaroo (ptk2) cells undergoing meiotic-like reduction division in vitro. | light and electron microscopy were used to study somatic cell reduction division occurring spontaneously in tetraploid populations of rat kangaroo potorous tridactylis (ptk2) cells in vitro. light microscopy coupled with time-lapse photography documented the pattern of reduction division which includes an anaphase-like movement of double chromatid chromosomes to opposite spindle poles followed by the organization of two separate metaphase plates and synchronous anaphase division to form four pol ... | 1977 | 556729 |
| the role of the centriolar region in animal cell mitosis. a laser microbeam study. | an argon ion laser microbeam (488 and 514 nm) was used to selectively irradiate one of the two centriolar regions of rat kangaroo potorous tridactylis (ptk2) prophase cells in vitro. the cells were sensitized to the laser radiation by treatment with acridine orange (0.1-0.2 mug/ml). ultrastructural examination of the irradiated centriolar regions demonstrated that the primary site of damage was the pericentriolar material. this result suggests that nucleic acid is present in the pericentriolar m ... | 1977 | 556728 |
| panting in small mammals: a comparison of two marsupials and the laboratory rabbit. | by use of a barometric technique, tidal volume (vt), minute volume (ve), respiratory frequency (f), and respiratory evaporative heat loss (eex) were measured from conscious unrestrained potoroos (potorous tridactylus), barred bandicoots (perameles gunnii), and new zealand white rabbits (oryctolagus cuniculus) at temperatures in and above the thermoneutral zone (tnz). rectal temperature (tre) and oxygen consumption were also measured. vt initially decreased with rising ta, but in the potoroo and ... | 1977 | 863814 |
| oxygen affinity and 2,3-diphosphoglycerate in blood of australian marsupials of differing body size. | oxygen carriage and 2,3-diphosphoglycerate (2,3-dpg) levels have been measured in the blood of seven species of australian marsupials ranging in size from 35 to 0.03 kg. they were red and grey kangaroos, wallaroo, tammar wallaby, brush-tailed possum, potoroo, and brown marsupial mouse. oxygen affinity decreased with decrease in adult body size, standard p50 (at 36 c) varying from 24.6 torr in the largest (red kangaroo) to 41.9 torr in the smallest (brown marsupial mouse). the relationship betwee ... | 1977 | 24866 |
| combined immunofluorescence and high-voltage electron microscopy of cultured mammalian cells, using an antibody that binds to glutaraldehyde-treated tubulin. | an antibody against tubulin that binds specifically to microtubules in glutaraldehyde-fixed cells has been prepared. sodium dodecyl sulfate-denatured tubulin treated with glutaraldehyde was used to immunize rabbits. in glutaraldehyde-fixed cells the fluorescent image of this antibody reveals a fine lattice of microtubules around the nucleus of ptk1 (potorous tridactylis) cells and many uniformly fluorescent microtubules in the peripheral cytoplasm. in cells fixed with formaldehyde the microtubul ... | 1978 | 415307 |
| damage and repair in mammalian cells after exposure to non-ionizing radiations. i. ultraviolet and visible light irradiation of cells of the rat kangaroo (potorous tridactylus) and determination of photorepairable damage in vitro. | 1978 | 672919 | |
| chromatin influence on the function and formation of the nuclear envelope shown by laser-induced psoralen photoreaction. | potorous tridactylis (ptk2) cells growing in culture were treated with psoralen derivatives and dividing cells were located by phase-contrast microscopy. psoralens, light-sensitive dna-photoadducting drugs, were reacted with mitotic chromosomes through exposure to 365-nm light from an argon laser microbeam system. it was found that following mitosis and photoreaction, cells without nuclear envelopes were produced when psoralen-treated cells received 60 light pulses over their entire chromosome c ... | 1978 | 701393 |
| the occurrence and structure of primary cilia in a subline of potorous tridactylus. | 1979 | 499374 | |
| damage and repair in mammalian cells after exposure to non-ionizing radiations. ii. photoreactivation and killing of rat kangaroo cells (potorous tridactylus) and herpes simplex virus-1 by exposure to fluorescent "white" light or sunlight. | photoreactivation (pr) of ultraviolet (254 nm)-inactivated cornea cells of the potoroo (or rat kangaroo; potorous tridacylus) has been studied at wavelengths greater than 375 nm from either fluorescent "white" light or sunlight. in both cases the pr kinetics curves pass through maxima, which most likely result from the superposition of concomitant inactivation by the photoreactivating light. the inactivating effect of light was directly demonstrated for non-uv-irradiated cells, permitting correc ... | 1980 | 6244485 |
| investigation of the determinants of nuclear pore number. | to assess the functional significance of nuclear pore complexes, we have investigated whether the number of pores per nucleus is determined by such factors as the nuclear volume, nuclear surface area, dna content, or aspects of nuclear activity. comparisons were made between cell types chosen to permit observation of differences in nuclear pore number as a function of differences in the other qualities measured. the number of nuclear pores was determined by freeze-etching and measurements of nuc ... | 1980 | 6966999 |
| morphological observations on the diapausing blastocyst of some macropodid marsupials. | diapausing blastocysts from the uteri of females of 11 macropodid species were fixed, torn, flattened and stained for examination of the protoderm. the protoderm remained undifferentiated and unilaminar throughout diapause. the number of cells in the protoderm did not vary with age in macropus rufus or m. eugenii. protoderm cell number decreased slightly with age in m. rufogriseus banksianus and appeared to decrease in potorous tridactylus. the diameter of the shell did not change appreciably wi ... | 1981 | 7205794 |
| mycobacterial osteomyelitis in captive marsupials. | mycobacterial osteomyelitis was detected in 3 marsupials exhibited at the national zoological park, washington, dc. one matschiei's tree kangaroo (dendrolagus matschiei), 1 parma wallaby (macropus parma), and 1 long-nosed rat kangaroo (potorous tridactylus) were affected. the parma wallaby had disseminated granulomatosis. acid-fast organisms were observed in the bone marrow of the wallaby, using the auramine-o-rhodamine fluorescent technique; however, cultures were negative. the tibiotarsal join ... | 1982 | 7174453 |
| assignment of snrna gene sequences to the large chromosomes of rat kangaroo and chinese hamster isolated by flow cytometric sorting. | chromosomes from a rat kangaroo (potorous tridactylus) cell line (ptk2) and from a chinese hamster (cricetulus griseus) cell line (chv79) were isolated by means of fluorescence activated flow cytometric sorting. dapi (4'-6-diamino-2-phenylindole) was used as the dna specific fluorescent dye. the karyotype of the ptk2 cells which exhibits 13 chromosomes was separated into 6, and the 22 chromosomes of the chv79 cells were resolved into 11 fractions. dna extracted from these chromosomal fractions w ... | 1982 | 6181947 |
| further observations on the potoroo (potorous tridactylus) in captivity. | the maintenance and breeding performance of potoroos in captivity over a 7-year period is described. by removing offspring from the pouch and allowing 29 days for completion of embryonic development and birth to occur, pouch young of known ages can be obtained. | 1983 | 6865321 |
| establishment of nucleolar deficient sublines of ptk2 (potorous tridactylis) by ultraviolet laser microirradiation. | one of the two nucleoli of tetraploid ptk2 wa cells in early prophase was irradiated with an ultraviolet (uv) laser microbeam. the daughter cells that maintained the nucleolar deficiency were isolated and cloned. five nucleolar deficient sublines of ptk2 wa were established, thus providing an experimental system to study the ribosomal gene-nucleolar organizer complex. | 1983 | 6840209 |
| the effects of hypoxia and co2 on the sleep-waking pattern of the potoroo (potorous tridactylus apicalis). | four male potoroos (potorous tridactylus apicalis) breathed 21% and 7% o2 with and without the addition of 5% co2. the effects of these gas mixtures on the potoroo's sleeping-waking pattern (swp) were studied. the swp while breathing 21% o2/5% co2 was unchanged when compared with that of breathing ambient air (21% o2). while breathing 7% o2, the swp was severely disrupted: total sleep time (tst) and slow wave sleep (sws) increased markedly. brain temperature fell substantially. paradoxical sleep ... | 1983 | 6405411 |
| morphologic evidence for fiber sorting in the fasciculus cuneatus. | this report describes the medullary course and projection patterns of cervical dorsal root nerve fibers in five mammalian species (bushbaby, tree squirrel, raccoon, potoroo, and brush-tailed possum). after cutting a single cervical dorsal root and allowing appropriate postoperative survival, we studied serial, fink-heimer impregnated sections of the medulla from each individual to locate degenerated afferent fibers and endings. fibers from those rostral (c2) or caudal (c8) cervical roots studied ... | 1984 | 6745379 |
| cycloheximide-sensitive recovery from 254 nm uv light damage in cultured marsupial cells. | colony-forming ability of potorous tridactylus-kidney (ptk-2) cells was measured after exposure to 254-nm ultraviolet (uv) light and cycloheximide. addition of 5 microm cycloheximide after exposure of the cells to uv light greatly decreased cell survival. maximum effect of the drug was obtained by 24-h exposure after irradiation. the cycloheximide sensitivity of irradiated cells was eliminated if addition of the drug was delayed for 8-10 h after irradiation, or if the cells received photoreactiv ... | 1984 | 6472330 |
| widespread cellular distribution of map-1a (microtubule-associated protein 1a) in the mitotic spindle and on interphase microtubules. | in the accompanying paper (bloom, g.s., t.a. schoenfeld, and r.b. vallee, 1983, j. cell biol. 98:320-330), we reported that microtubule-associated protein 1 (map 1) from brain comprises multiple protein species, and that the principal component, map 1a, can be detected in both neuronal and glial cells by immunofluorescence microscopy using a monoclonal antibody. in the present study, we sought to determine the cellular and subcellular distribution of map 1a in commonly used cultured cell systems ... | 1984 | 6142895 |
| the dorsal lateral geniculate nucleus of macropodid marsupials: cytoarchitecture and retinal projections. | the anatomy of the dorsal lateral geniculate nucleus (lgd) is described in five macropodid species, including two rat kangaroos (bettong and potoroo), two wallabies (pademelon and tammar), and the large grey kangaroo. the distribution of retinal terminals in the lgd was examined following intraocular injections of tritiated amino acids. there are considerable differences in both lgd cytoarchitecture and the patterns of retinal terminations among the five species. cytoarchitecture in the bettong ... | 1984 | 6715581 |
| comparative studies on photoreactivation of ultraviolet light-induced t4 endonuclease susceptible sites and sister-chromatid exchanges in potorous cells. | photoreactivation (pr) of t4 endonuclease-susceptible sites (ess) and sister-chromatid exchanges induced by ultraviolet light was investigated in potorous tridactylis pt k2 cells, using monochromatic light from a grating monochromator. both ess and sce showed maximum pr at 350 nm and the action spectra of pr essentially overlapped between ess and sce at 350, 400 and 450 nm. exposure to 325-nm light after uv irradiation induced additional ess and sce, but reduction of ess was shown by increasing ... | 1985 | 4000171 |
| effect of cadmium on the reproductive organs of the male potoroo potorous tridactylus (macropodidae). | low (2.28 mg/kg) or high (4.56 mg/kg) doses of cadmium chloride were administered, either intraperitoneally or subcutaneously, to adult male potoroos (p. tridactylus). after 7 days, the testes, caput epididymis and spermatic cords of the high-dose groups all displayed a degree of cellular damage; however, the damage was not as extensive as that which occurs in some eutherian species after only 24 h. this time differential might be attributed to structural differences between the testicular blood ... | 1985 | 4091756 |
| growth and role of the corpus luteum throughout delayed gestation in the potoroo, potorous tridactylus. | the growth cycle of the corpus luteum (cl) of the potoroo is similar to that of other macropodids. during delayed gestation, the post-partum cl remains quiescent until it is reactivated by removal of the sucking pouch young. the cl then undergoes a period of growth, rapid from day 6 until day 12, followed by a gradual decline from day 21 to day 27. excision of the cl before day 6 of pregnancy either inhibited embryonic development or failed to support it. excision of the cl between 6 and 21 days ... | 1986 | 3944807 |
| rna polymerase activity in ptk1 micronuclei containing individual chromosomes. an in vitro and in situ study. | micronuclei have been induced by colchicine in rat kangaroo (potorous tridactylis) ptk1 cells. the synthesis of rna was investigated both in isolated micronuclei by quantifying rna polymerase activities at different ionic strengths with or without inhibitors, and in micronucleated cells by radioautography after [3h]uridine pulse labeling. in vitro transcription shows that isolated micronuclei are able to take up [3h]utp. the rate curves of incorporation are close to those of isolated diploid nuc ... | 1987 | 3817015 |
| posttranslational modification and microtubule stability. | we have probed the relationship between tubulin posttranslational modification and microtubule stability, using a variation of the antibody-blocking technique. in human retinoblastoma cells we find that acetylated and detyrosinated microtubules represent congruent subsets of the cells' total microtubules. we also find that stable microtubules defined as those that had not undergone polymerization within 1 h after injection of biotin-tubulin were all posttranslationally modified; furthermore dyna ... | 1987 | 3316248 |
| levels of synthesis of primate-specific nuclear proteins differ between growth-arrested and proliferating cells. | a monoclonal antibody that reacts specifically with the proliferation-sensitive nuclear proteins, isoelectric focusing (ief) 8z30 and 8z31 (molecular weight (mw), 76,000 charge variants, hela protein catalogue number) has been characterized. as determined by indirect immunofluorescence, the antibody stains the nucleolus and nucleoplasm of interphase-cultured cells of primate origin, but does not react with cells of other species. proteins having similar mws and isoelectric points as the human or ... | 1987 | 3542540 |
| evidence that the 90-kilodalton heat shock protein is associated with tubulin-containing complexes in l cell cytosol and in intact ptk cells. | it has been established that the 90-kilodalton murine heat shock protein, hsp90, is associated with the untransformed, non-dna-binding form of the glucocorticoid receptor in l cell cytosol. in this work, we show that incubation of l cell cytosol with affi-gel-coupled monoclonal antibodies directed against either alpha-tubulin alone or both alpha- and beta-tubulin results in the immune-specific adsorption of hsp90 identified by western blotting with the ac88 monoclonal antibody. similarly, the ac ... | 1988 | 3062385 |
| comparative lactation in two species of rat-kangaroo (marsupialia). | 1. milk composition and energy content was measured in two related marsupials, bettongia gaimardi and potorous tridactylus. 2. although protein content was consistently higher in bettong milk, lactational trends in both species are similar to those reported for other marsupials. 3. the faster relative growth rate of the bettong may be correlated with the increased protein and a higher milk intake. | 1988 | 2901316 |
| propagation and quantitation of animal herpesviruses in eight cell culture systems. | a comparative study was carried out to determine the relative sensitivities of eight different cell culture systems to six different herpesviruses of animals. the cells used were: ofl (ovine fetal lung), ml (mink lung), fk (ferret kidney), ptk-2 (potoroo kidney), tek (turkey embryo kidney), ed (equine dermal), bt (bovine turbinate), and pk15 (porcine kidney). the viruses tested were: prv (pseudorabies) of swine, cphv (caprine herpesvirus), ibrv (infectious bovine rhinotracheitis virus), dn-599 s ... | 1988 | 2846231 |
| eimeria spp. (apicomplexa:eimeriidae) parasitic in the rat-kangaroos hypsiprymnodon moschatus, potorous tridactylus, aepyprymnus rufescens and bettongia gaimardi (marsupialia:potoroidae). | 1988 | 3225116 | |
| radiation-induced recovery processes in cultured marsupial cells. | the ultraviolet sensitivity of potorous tridactylus male kidney (ptk-2) cells is markedly increased by post irradiation treatment for 24 h with 5 microm emetine (which inhibits protein synthesis by acting on the 40s ribosomal subunit), or with 5 microm cycloheximide (which inhibits by interaction with the 60s subunit), or with the rna polymerase ii inhibitor 5,6-dichloro-1-beta-ribofuranosylbenzimidazole at 50 microm. all 3 treatments give the same sensitivity, while unirradiated cells are littl ... | 1988 | 3412365 |
| recovery of viral capacity in irradiated exponentially growing cells. | exponentially growing cells of the ptk-2 line (atcc no. ccl56, from the marsupial potorous tridactylus) require protein and rna synthesis in a limited period following uv-radiation damage for optimal recovery as colony formers [overberg et al. (1988) mutat. res. 194, 83-92]. overall behavior suggests the operation of damage-induced recovery processes. the capacity of confluent cell monolayers for infection with unirradiated herpes simplex virus 1 (hsv-1) is sharply reduced by uv-irradiation. we ... | 1989 | 2548221 |
| a tubulin identified by a monoclonal antibody is not present in mitotic spindles. | a monoclonal antibody, g8, which recognizes a form of tubulin (g8-tubulin) with a novel distribution in rat-1 cells and potorous tridactylis kidney (ptk-2) cells was isolated. g8 labeled the interphase cytoskeleton of rat-1 fibroblasts but not mitotic spindles or midbodies. g8 also stained a fiber network in some but not all ptk-2 interphase cells but did not label mitotic spindles or midbodies in these cells. g8-tubulin is the only identified tubulin known to be absent from these structures. th ... | 1989 | 2806400 |
| in vitro photosensitization ii. an electron microscopy study of cellular destruction with mono-l-aspartyl chlorin e6 and photofrin ii. | primary sites of subcellular destruction with photofrin ii (pfii) and mono-l-aspartyl chlorin e6 (mace) were determined by transmission electron microscopy (tem). potorous tridactylus (ptk2) cells were grown in rose chambers and incubated for 24 hr with a sensitizer concentration sufficient to provide 100% mortality. cells were irradiated with laser light and fixed and processed for electron microscopy at various times post-irradiation. the results indicate that pfii initially destroys mitochond ... | 1989 | 2523992 |
| laminar and non-laminar patterns of acetylcholinesterase activity in the marsupial lateral geniculate nucleus. | acetylcholinesterase (ache) distribution in the dorsal lateral geniculate nucleus (lgd) of 3 polyprotodont and 3 diprotodont marsupials has been compared with the cytoarchitecture and, as appropriate, with retinal terminal bands (rtbs) as demonstrated by tracers injected into the vitreous body. in this series those polyprotodont marsupials showing only partial segregation of retinal input (virginia opossum and tasmanian devil), display the least cell laminar differentiation. in them ache stainin ... | 1989 | 2731033 |
| photoreactivation of ultraviolet radiation-induced release of arachidonic acid from marsupial cells. | exposure of an established marsupial cell line, ptk2 (potorous tridactylus), to ultraviolet radiation (uvr) from an fs-40 sunlamp (280-400 nm) resulted in a fluence-dependent release of radiolabeled arachidonic acid (aa) from cell membranes. post-uvr, but not pre-uvr, exposure to photoreactivating light reversed uvr-induced pyrimidine dimers in dna and suppressed the uvr-induced release of aa. these data indicate that dna damage contributes to the release of aa from membrane phospholipids. | 1991 | 1665911 |
| relationships between plasma composition and parotid salivary composition and secretion rates in the potoroine marsupials, aepyprymnus rufescens and potorous tridactylus. | parotid salivation was investigated in two species of potoroine marsupial, aepyprymnus rufescens and potorous tridactylus to ascertain flow rates and composition, the buffer capacity of the saliva with respect to possible dependence of these animals on foregut fermentation, and the similarity of anion excretion patterns to those of the kangaroo parotid. under anaesthesia neither species secreted spontaneously and secretion was stimulated by intravenous infusion of carbachol, bethanechol and isop ... | 1992 | 1281847 |
| detection of the 125-kda nuclear protein mitotin in centrosomes, the poles of the mitotic spindle, and the midbody. | mitotin is a nuclear protein detectable in all proliferating cells investigated so far, including human and plant cells. in interphase cells the protein is localized mainly in the nucleoplasm. in g2/m phase it displays a characteristic redistribution and a marked increase which initiated the name mitotin. this study presents the precise localization of mitotin in cytoplasmic structures in two cell types, the potoroo rat kangaroo ptk2 cell and the human lung cancer eplc 65 cell. in addition to it ... | 1992 | 1544381 |
| energy metabolism in potoroine marsupials. | fasting and fed metabolic rates were measured in three species of potoroine marsupials, the rufous rat-kangaroo (aepyprymnus rufescens), the long-nosed potoroo (potorous tridactylus) and the brush-tailed bettong (bettongia penicillata). there were no significant differences among potoroine species in fasting metabolic rate. the lowest fasting heat production for each species was 11-20% less than the interspecific value of 295 kj.kg-0.75.day-1 for basal metabolism of mature, non-reproductive euth ... | 1992 | 1401340 |
| optical trapping for chromosome manipulation: a wavelength dependence of induced chromosome bridges. | using a tunable titanium-sapphire laser, we have compared different wavelengths (from 700 to 840 nm) for their utility in optical trapping of chromosomes in mitotic rat kangaroo potorous tridactylus (ptk2) cells. it was found that irradiation with a near-infrared light induces the sticking together of chromosome shoulders. the attached chromatids failed to separate, or separated with significant delay and formed a chromosome bridge during anaphase. using this bridge (and induced c-mitosis) as a ... | 1993 | 8457677 |
| energetic cost of locomotion as a function of ambient temperature and during growth in the marsupial potorous tridactylus. | in the marsupial, the potoroo, multiple regression analysis shows that ambient temperature makes a minor (2%) contribution towards variation in oxygen consumption with speed. this suggests that the heat generated during running is substituted for heat which would otherwise have to be generated for temperature regulation. maximum levels of oxygen consumption are also temperature-independent over the range 5-25 degrees c, but plasma lactate concentrations at the conclusion of exercise significantl ... | 1993 | 8440970 |
| a new class of dna photolyases present in various organisms including aplacental mammals. | dna photolyase specifically repairs uv light-induced cyclobutane-type pyrimidine dimers in dna through a light-dependent reaction mechanism. we have obtained photolyase genes from drosophila melanogaster (fruit fly), oryzias latipes (killifish) and the marsupial potorous tridactylis (rat kangaroo), the first photolyase gene cloned from a mammalian species. the deduced amino acid sequences of these higher eukaryote genes show only limited homology with microbial photolyase genes. together with th ... | 1994 | 7813451 |
| a monoclonal antibody epitope on keratin 8 identified using a phage peptide library. | a bacteriophage random hexapeptide library was used to define the epitope of a monoclonal anti-keratin antibody. phage selected by the keratin 8-specific antibody le41 displayed highly related sequences on their piii coat protein. the consensus sequence s(x)lnp allowed the precise localization of an le41 epitope (sllsp) within the head domain (h1 subdomain) of human keratin 8, known to be important for correct filament polymerisation. by sequencing the immunizing antigen, keratin 8 from potorous ... | 1994 | 7507169 |
| myosin is involved in postmitotic cell spreading. | we have investigated a role for myosin in postmitotic potoroo tridactylis kidney (ptk2) cell spreading by inhibitor studies, time-lapse video microscopy, and immunofluorescence. we have also determined the spatial organization and polarity of actin filaments in postmitotic spreading cells. we show that butanedione monoxime (bdm), a known inhibitor of muscle myosin ii, inhibits nonmuscle myosin ii and myosin v adenosine triphosphatases. bdm reversibly inhibits ptk2 postmitotic cell spreading. lis ... | 1995 | 7559774 |
| ultraviolet-induced cell death is independent of dna replication in rat kangaroo cells. | rat kangaroo (potorous tridactylus) cells have an efficient repair system for photoreactivation of lethal lesions induced by 254 nm uv. however, this ability is lost with increasing time after uv, being completely ineffective after 24 h. critical events leading to uv-induced cell death must occur within this period of time. dna synthesis was inhibited by the dna polymerase inhibitor aphidicolin and the loss of the capability to photorepair lethal lesions was maintained as for replicating cells. ... | 1995 | 7770506 |
| ultrastructure and motility of spermatozoa in macropodid and potoroidid marsupials. | in order to gain some understanding of the significance of the morphological features of spermatozoa within the macropodoidea, the motility of spermatozoa from two macropodids (petrogale xanthopus and dendrolagus matschiei) and the motility, number and distribution of spermatozoa from three potoroidids (aepyprymnus rufescens, bettongia penicillata and potorous tridactylus) were examined. sperm were collected by electro-ejaculation or from the cauda epididymides. epididymides from the potoroidids ... | 1995 | 8848581 |
| effect of cooling and cryopreservation on sperm motility and morphology of several species of marsupial. | the effects of long-term cooling and freezing on sperm motility are described for six marsupial species: the fat-tailed dunnart, koala, brushtail possum, long-footed potoroo, northern brown bandicoot and ring-tailed possum. the effects of up to eight days of cooling at 4 degrees c on the motility of dunnart spermatozoa and the effect of cryopreservation on spermatozoa of the other species were determined. the cryoprotectant used was a tris-citrate-fructose-egg yolk-glycerol diluent. the percenta ... | 1996 | 8870089 |
| adaptive changes in trabecular architecture in relation to functional strain patterns and disuse. | principal strains and their orientation, determined from in vivo and in situ strains recorded from the lateral cortical surface of the calcaneus of potoroos (a small marsupial) during treadmill exercise and tension applied via the achilles tendon, were compared with the underlying trabecular architecture and its alignment to test wolff's "trajectorial theory" of trabecular alignment. in vivo and in situ principal compressive strains (-800 to -2000 mu e) were found to be aligned (mean 161 +/- 7 d ... | 1996 | 8830980 |
| interspecies diversity of the occludin sequence: cdna cloning of human, mouse, dog, and rat-kangaroo homologues. | occludin has been identified from chick liver as a novel integral membrane protein localizing at tight junctions (furuse, m., t. hirase, m. itoh, a. nagafuchi, s. yonemura, sa. tsukita, and sh. tsukita. 1993. j. cell biol. 123:1777-1788). to analyze and modulate the functions of tight junctions, it would be advantageous to know the mammalian homologues of occludin and their genes. here we describe the nucleotide sequences of full length cdnas encoding occludin of rat-kangaroo (potoroo), human, m ... | 1996 | 8601611 |
| activities of the microtubule-stabilizing agents epothilones a and b with purified tubulin and in cells resistant to paclitaxel (taxol(r)). | epothilones a and b, natural products with minimal structural analogy to taxoids, have effects similar to those of paclitaxel (taxol(r)) in cultured cells and on microtubule protein, but differ from paclitaxel in retaining activity in multidrug-resistant cells. we examined interactions of the epothilones with purified tubulin and additional cell lines, including a paclitaxel-resistant ovarian carcinoma line with an altered beta-tubulin. the epothilones, like paclitaxel, induced tubulin to form m ... | 1997 | 8999970 |
| the microtubule-stabilizing agent discodermolide competitively inhibits the binding of paclitaxel (taxol) to tubulin polymers, enhances tubulin nucleation reactions more potently than paclitaxel, and inhibits the growth of paclitaxel-resistant cells. | the lactone-bearing polyhydroxylated alkatetraene (+)-discodermolide, which was isolated from the sponge discodermia dissoluta, induces the polymerization of purified tubulin with and without microtubule-associated proteins or gtp, and the polymers formed are stable to cold and calcium. these effects are similar to those of paclitaxel (taxol), but discodermolide is more potent. we confirmed that these properties represent hypernucleation phenomena; we obtained lower tubulin critical concentratio ... | 1997 | 9380024 |
| investigation of the mechanism of retraction of the cell margin and rearward flow of nodules during mitotic cell rounding. | we have studied two types of cell motility directed toward the cell center: retraction of the cell margin and rearward flow of small cytoplasmic nodules during mitotic cell rounding in potoroo tridactylis kidney (ptk2) cells by time-lapse video microscopy, drug treatments, and photoactivation of fluorescence. nodules flow rearward on thin, actin-rich fibers (retraction fibers) exposed as the cell margin retracts. retraction of the cell margin and rearward flow of nodules require intact actin fil ... | 1997 | 9017599 |
| characterization of microsatellite loci in the endangered long-footed potoroo potorous longipes. | 1997 | 9161018 | |
| the chondramides: cytostatic agents from myxobacteria acting on the actin cytoskeleton. | chondramides are cyclodepsipeptides produced by strains of the myxobacterium, chondromyces crocatus. these peptides, which have been reported to inhibit yeast and mammalian cell proliferation, are related to jasplakinolide, which has been isolated from marine sponges of the genus jaspis and has been shown to interfere with the actin cytoskeleton (a structural component of cells that helps maintain their shape and is involved in processes, such as cell division and cell locomotion). we studied th ... | 1998 | 9790549 |
| non-shivering thermogenesis in the potoroo, potorous tridactylus (kerr). | 1998 | 24519 | |
| the phylogenetic position of the musky rat-kangaroo and the evolution of bipedal hopping in kangaroos (macropodidae: diprotodontia). | kangaroos and their relatives (family macropodidae) are divided into the subfamilies macropodinae (kangaroos, wallabies, pademelons) and potoroinae (rat-kangaroos, potoroos, bettongs). the musky rat-kangaroo, hypsiprymnodon moschatus, is traditionally allied with other potoroines, based primarily on the basis of osteological characters and aspects of the female reproductive system. unlike other macropodids, however, which are capable of bipedal hopping, hypsiprymnodon is a quadrupedal bounder an ... | 1998 | 12066687 |
| [characteristics of quantitative karyotypic variability in cell line of kidney from rat kangaroo (potorous tridactylis)]. | the numerical regulations of karyotypic variability in cell line of rat kangaroo kidney, nbl-3-11, has been investigated. these regulations are similar with ones found for cell lines of the indian muntjac skin fibroblasts (m, mt, m2). in particular the balanced karyotypic structure of cell population in vitro is determined by correlations of the structural variants of the karyotype (svk). these correlations depend on the following regulations 1) nonrandom character of cell distribution according ... | 1999 | 10591122 |
| effects of rhizopodin and latrunculin b on the morphology and on the actin cytoskeleton of mammalian cells. | the effects of the novel myxobacterial compound rhizopodin on mammalian cells were studied and compared with those of latrunculin b. both substances induced adherently growing l929 mouse fibroblasts and ptk2 potoroo kidney cells to produce long, narrow, branched extensions or runners. rhizopodin was more efficient than latrunculin b in that respect (minimal inhibitory concentration with l929 cells 5 nm vs 50 nm), and, in contrast to latrunculin b, its effects were permanent. rhizopodin-treated c ... | 1999 | 9931358 |
| [characteristics of quantitative karyotypic variability in cell line of kidney from rat kangaroo (potorous tridactylis)]. | the goal of this work was to investigate the numeral karyotypic variability in different sublines (mt, m2). these sublines are formed spontaneously from the main cell line (m) and have modal number of chromosomes 9 and 10, msvk (main structural variant karyotype)--3 + 3 + 1 + 2 and 3 + 4 + 2 + 1. there are general regulations which were originally got for the line m. in particular: 1) nonrandom character of cell distribution according to the number of chromosomal deviations from msvk; 2) specifi ... | 1999 | 10591120 |
| karyotype relationships between four distantly related marsupials revealed by reciprocal chromosome painting. | marsupial karyotypes have shown extensive conservation even between distantly related groups with a high diversity of life forms and reproductive biology. banding analysis has been the main test for assessing their homologies and chromosome rearrangements. more recently, cross-species reciprocal chromosome painting has been developed and applied to several mammalian species and has shown homologies and rearrangements not revealed by banding analysis. karyotype relationships between four marsupia ... | 1999 | 10560969 |
| photorepair prevents ultraviolet-induced apoptosis in human cells expressing the marsupial photolyase gene. | photolyase absorbs blue light and employs the energy to remove uv-induced dna damage, cyclobutane pyrimidine dimers, or pyrimidine pyrimidone (6-4) lesions. these enzymes have been found in many living organisms ranging from bacteria to aplacental mammals, but their photoreactivation effect, such as survival increase of uv-irradiated cells by light-illumination, has not been identified in placental mammals, including humans. therefore, we introduced a photolyase gene derived from the marsupial r ... | 2000 | 10811124 |
| length control of primary cilia: analysis of monociliate and multiciliate ptk1 cells. | lengths of primary cilia in cultured ptk1 cells, on both a population basis and within individual (multiciliate) cells, have been compared. the latter examines the degree of discrepancy between cilia arising within the same cell and using a common precursor pool, on the hypothesis that a better correlation would be expected between cilia issuing from the same centrosome than between those in the population in general. to obtain accurate et plentiful measurements, a 'flow-fixation' technique was ... | 2000 | 11374436 |
| cloning of human, murine, and marsupial keratin 7 and a survey of k7 expression in the mouse. | keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. keratin 7 (k7) is expressed in a wide range of epithelial structures in humans. we have cloned and fully sequenced the human and mouse k7 genes and mrnas, and the k7 mrna from the marsupial potorous tridactylis, from which the widely used simple epithelial cell lines ptk1 and ptk2 are derived. percentage identity plots comparing the mouse and human genomic sequences revealed a number of conserved cpg islands a ... | 2002 | 12359226 |
| localization of chromosome regions in potoroo nuclei ( potorous tridactylus marsupialia: potoroinae). | chromosome paints of the rat kangaroo ( aepyprymnus rufuscens, 2 n=32) were used to define chromosome regions in the long nosed potoroo ( potorous tridactylus, 2 n=12 female, 13 male) karyotype and localize these regions in three-dimensionally preserved nuclei of the potoroo to test the hypothesis that marsupial chromosomes have a radial distribution. in human nuclei chromosomes are distributed in a proposed radial fashion. gene-rich chromosomes in the human interphase nucleus are preferentially ... | 2003 | 12844220 |
| archazolids, new cytotoxic macrolactones from archangium gephyra (myxobacteria). production, isolation, physico-chemical and biological properties. | novel cytotoxic compounds, archazolid a and b, were isolated from the culture broth of strains of the myxobacteria archangium gephyra and cystobacter sp. archazolids consist of a macrocyclic lactone ring with a thiazole side chain and showed high activity against mammalian cells. the ic50 values with different cell lines ranged from 0.1 to 1 ng/ml. an incubation of ptk2 potoroo cells with archazolid a (5 ng/ml) led to the formation of vacuoles in the er, a phenomenon that is typical for inhibito ... | 2003 | 12931860 |
| wide range of chlamydiales types detected in native australian mammals. | the chlamydiales are a unique order of intracellular bacterial pathogens that cause significant disease of birds and animals, including humans. the recent development of a chlamydiales-specific 16s rdna polymerase chain reaction (pcr) assay has enabled the identification of chlamydiales dna from an increasing range of hosts and environmental sources. whereas the australian marsupial, the koala, has previously been shown to harbour several chlamydiales types, no other australian marsupials have b ... | 2003 | 14519335 |
| an analysis of chromosomal sensitivity of hpk1 cells from the marsupial potorous tridactylus to x-rays and to heliotrine. | 2003 | 5065177 | |
| controlled ablation of microtubules using a picosecond laser. | the use of focused high-intensity light sources for ablative perturbation has been an important technique for cell biological and developmental studies. in targeting subcellular structures many studies have to deal with the inability to target, with certainty, an organelle or large macromolecular complex. here we demonstrate the ability to selectively target microtubule-based structures with a laser microbeam through the use of enhanced yellow fluorescent protein (eyfp) and enhanced cyan fluores ... | 2004 | 15454403 |
| molecular phylogenetic relationships of two extinct potoroid marsupials, potorous platyops and caloprymnus campestris (potoroinae: marsupialia). | complete 12s rrna and partial cytochrome b (cytb) gene sequences have been obtained from museum samples of two recently extinct potoroids-potorous platyops and caloprymnus campestris. phylogenetic analyses based on these mitochondrial dna sequences suggest that the broad-faced potoroo (p. platyops) was a close relative of the recently discovered potorous longipes and the recently re-discovered potorous gilberti. although the extinct desert rat-kangaroo (c. campestris) was clearly resolved as a m ... | 2004 | 15062789 |
| a centromere-specific retroviral element associated with breaks of synteny in macropodine marsupials. | studies of chromosome evolution have focused heavily on the evolution of conserved syntenic, gene-rich domains. it is obvious, however, that the centromere plays an equally important role in chromosome evolution, through its involvement in fissions, centric fusions, translocations, inversions and centric shifts. it is unclear how the centromere, either as a functioning unit of the chromosome or as a dna sequence motif, has been involved in these processes. marsupials of the family macropodidae ( ... | 2004 | 15305065 |
| protection after x-irradiation by 1,4-dithiothreitol of two mammalian cell types in vitro. | 1,4-dithiothreitol (dtt) was found to diminish the amount of chromosomal breakage in leucocyte cultures of the marsupial potorous tridactylus and in the don strain of chinese hamster cells (lhi). the maximum decrease of damage in potorous lymphocytes was to 54% of that of the irradiated cells without dtt treatment, and to 64% in the case of the chinese hamster cells. for both cell types, it was shown that the most significant effect of dtt was due to its presence in the first 30 min post irradia ... | 2005 | 546598 |
| genomic instability within centromeres of interspecific marsupial hybrids. | several lines of evidence suggest that, within a lineage, particular genomic regions are subject to instability that can lead to specific types of chromosome rearrangements important in species incompatibility. within family macropodidae (kangaroos, wallabies, bettongs, and potoroos), which exhibit recent and extensive karyotypic evolution, rearrangements involve chiefly the centromere. we propose that centromeres are the primary target for destabilization in cases of genomic instability, such a ... | 2007 | 18073443 |
| fluorescence correlation spectroscopy investigation of a gfp mutant-enhanced cyan fluorescent protein and its tubulin fusion in living cells with two-photon excitation. | this study investigates the feasibility of using the enhanced cyan mutant of green fluorescent protein (ecfp) as a probe for two-photon fluorescence correlation spectroscopy (fcs). molecular dynamics and other properties of ecfp and an ecfp-tubulin fusion protein were investigated in living potorous tridactylis (ptk2) cells. ecfp has high molecular brightness in the nucleus (eta=3.3 kcpsm) and in the cytoplasm (3.2 kcpsm) under our experimental conditions. the diffusion constants of ecfp were de ... | 2007 | 15065907 |