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differential cytolocation and functional assays of the two major human slc30a8 (znt8) isoforms.the non-synonymous single nucleotide polymorphism (snp) rs13266634 in human zinc transporter 8, znt8 (slc30a8), leads to a r325 variant, which is associated with an increased risk of developing type 2 diabetes (t2d). although the molecular details remain unknown, the mutation is thought to alter the kinetics of zinc transport into insulin granules in pancreatic β-cells. nevertheless, analysis of znt8 sequences from several animals shows that the amino acid at position 325 is poorly conserved. ap ...201728965566
effect of allyl isothiocyanate on developmental toxicity in exposed xenopus laevis embryos.the pungent natural compound allyl isothiocyanate isolated from the seeds of cruciferous (brassica) plants such as mustard is reported to exhibit numerous beneficial health-promoting antimicrobial, antifungal, anticarcinogenic, cardioprotective, and neuroprotective properties. because it is also reported to damage dna and is toxic to aquatic organisms, the objective of the present study was to determine whether it possesses teratogenic properties. the frog embryo teratogenesis assay-xenopus (fet ...201528962355
sequence and timing of early cranial skeletal development in xenopus laevis.xenopus laevis is widely used as a model organism in biological research. morphological descriptions of the larval cartilaginous skeleton are more than half a century old and comprehensive studies of early cartilage differentiation and development are missing. a proper understanding of early cranial skeletal development in x. laevis requires a detailed description that can function as a baseline for experimental studies. this basis makes it possible to evaluate skeletal defects produced by exper ...028960402
a comparison of electronic and traditional cigarette butt leachate on the development of xenopus laevis embryos.potential developmental toxicities of three different cigarette butt leachates were evaluated using the frog embryo teratogenesis assay-xenopus (fetax). xenopus laevis embryos were exposed to regular cigarette butt (rcb), menthol (mcb) and electronic (ecb) in concentrations ranging from 0 to 4 butts/l for rcb and mcb and 0-10 butts/l for ecb. the embryos were from stage 8 to 11 and were exposed for a 96-h period in static renewal test conditions. median lethal concentration (lc50), malformation ...201728959628
e-cigarette aerosol exposure can cause craniofacial defects in xenopus laevis embryos and mammalian neural crest cells.since electronic cigarette (ecig) introduction to american markets in 2007, vaping has surged in popularity. many, including women of reproductive age, also believe that ecig use is safer than traditional tobacco cigarettes and is not hazardous when pregnant. however, there are few studies investigating the effects of ecig exposure on the developing embryo and nothing is known about potential effects on craniofacial development. therefore, we have tested the effects of several aerosolized e-ciga ...201728957438
expression and purification of the full murine npm2 and study of its interaction with protamines and histones.mouse nucleoplasmin m.npm2 was recombinantly expressed and the protein consisting of the complete sequence was purified and characterized. similar to its xenopus laevis x.npm2 counterpart, the protein forms stable pentameric complexes and exhibits an almost undistinguishable hydrodynamic ionic strength-dependent unfolding behavior. the interaction of n.pm2 with histones and mouse p1/p2 protamines revealed that these chromosomal proteins bind preferentially to the distal part of the nucleoplasmin ...201628955874
complicated behavior of g-quadruplexes and evaluating g-quadruplexes' ligands in various systems mimicking cellular circumstance.environments surrounding g-rich sequences remarkably affect the conformations of these structures. a proper evaluation system mimicking the crowded environment in a cell with macromolecules should be developed to perform structural and functional studies on g-quadruplexes. in this study, the topology and stability of a g-quadruplex formed by human telomeric repeat sequences were investigated in a macromolecule-crowded environment created by polyethylene glycol 200 (peg200), tumor cell extract, a ...201628955851
six1 and eya1 both promote and arrest neuronal differentiation by activating multiple notch pathway genes.the transcription factor six1 and its cofactor eya1 are important regulators of neurogenesis in cranial placodes, activating genes promoting both a progenitor state, such as hes8, and neuronal differentiation, such as neurog1. here, we use gain and loss of function studies in xenopus laevis to elucidate how these genes function during placodal neurogenesis. we first establish that hes8 is activated by notch signaling and represses neurog1 and neuronal differentiation, indicating that it mediates ...201728947179
mammalian odorant receptor tuning breadth persists across distinct odorant panels.the molecular receptive range (mrr) of a mammalian odorant receptor (or) is the set of odorant structures that activate the or, while the distribution of these odorant structures across odor space is the tuning breadth of the or. variation in tuning breadth is thought to be an important property of ors, with the mrrs of these receptors varying from narrowly to broadly tuned. however, defining the tuning breadth of an or is a technical challenge. for practical reasons, a screening panel that broa ...201728945824
tumor immunology viewed from alternative animal models-the xenopus story.nonmammalian comparative animal models are important not only to gain fundamental evolutionary understanding of the complex interactions of tumors with the immune system, but also to better predict the applicability of novel immunotherapeutic approaches to humans. after reviewing recent advances in developing alternative models, we focus on the amphibian xenopus laevis and its usefulness in deciphering the perplexing roles of mhc class i-like molecules and innate (i)t cells in tumor immunity.201728944105
the brain is required for normal muscle and nerve patterning during early xenopus development.possible roles of brain-derived signals in the regulation of embryogenesis are unknown. here we use an amputation assay in xenopus laevis to show that absence of brain alters subsequent muscle and peripheral nerve patterning during early development. the muscle phenotype can be rescued by an antagonist of muscarinic acetylcholine receptors. the observed defects occur at considerable distances from the head, suggesting that the brain provides long-range cues for other tissue systems during develo ...201728943634
identification of regions responsible for the function of the plant k+ channels kat1 and akt2 in saccharomyces cerevisiae and xenopus laevis oocytes.the arabidopsis k+ channel kat1 complements in k+-limited medium the growth of the k+ uptake defective saccharomyces cerevisiae mutant strain cy162, while another k+ channel, akt2, does not. to gain insight into the structural basis for this difference, we constructed 12 recombinant chimeric channels from these two genes. when expressed in cy162, only three of these chimeras fully rescued the growth of cy162 under k+-limited conditions. we conclude that the transmembrane core region of kat1 is i ...201728933647
the nitrate transporter family protein ljnpf8.6 controls the n-fixing nodule activity.n-fixing nodules are new organs formed on legume roots as a result of the beneficial interaction with soil bacteria, rhizobia. the nodule functioning is still a poorly characterized step of the symbiotic interaction, as only a few of the genes induced in n-fixing nodules have been functionally characterized. we present here the characterization of a member of the lotus japonicus nitrate transporter1/peptide transporter family, ljnpf8.6 the phenotypic characterization carried out in independent l ...201728931627
beyond mouse cancer models: three-dimensional human-relevant in vitro and non-mammalian in vivo models for photodynamic therapy.the proper design of experiments is a critical step for each study in order to obtain reproducible and reliable data. taking into account constant competitiveness in the quickly developing biomedical sciences and the availability of sophisticated techniques, the choice and establishment of an experimental model system are essential for a successful research project. currently, various sophisticated in vitro and in vivo models are being designed and developed in order to replace the use of mammal ...201728927532
expression and functional proteomic analyses of osteocytes from xenopus laevis tested under mechanical stress conditions: preliminary observations on an appropriate new animal model.hitherto, the role of the osteocyte as transducer of mechanical stimuli into biological signals is far from settled. in this study, we used an appropriate model represented by the cortex of xenopus laevis long bone diaphysis lacking (unlike the mammalian one) of vascular structures and containing only osteocytes inside the bone matrix. these structural features allow any change of protein profile that might be observed upon different experimental conditions, such as bone adaptation to stress/mec ...201728925539
β subunits control the effects of human kv4.3 potassium channel phosphorylation.the transient outward k+ current, ito, activates early in the cardiac myocyte action potential, to begin repolarization. human ito is generated primarily by two kv4.3 potassium channel α subunit splice variants (kv4.3l and kv4.3s) that diverge only by a c-terminal, membrane-proximal, 19-residue stretch unique to kv4.3l. protein kinase c (pkc) phosphorylation of threonine 504 within the kv4.3l-specific 19-residues mediates α-adrenergic inhibition of ito in human heart. kv4.3 is regulated in human ...201728919864
hmg-box factor soxd/sox15 and homeodomain-containing factor xanf1/hesx1 directly interact and regulate the expression of xanf1/hesx1 during early forebrain development in xenopus laevis.the homeodomain-containing transcription factor anf (also known as rpx/hesx1 in mammals) plays an important role during the forebrain development in vertebrates. here we demonstrate the ability of the xenopus laevis anf, xanf1/hesx1, to directly bind sry-related hmg-box-containing transcription factor soxd/sox15 and to cooperate with the latter during regulating of the expression of xanf1/hesx1 own gene. as we have shown by gst pull-down, emsa and the luciferase reporter assays, xanf1/hesx1 and ...028918251
molecular cloning and functional expression of the k+ channel kv7.1 and the regulatory subunit kcne1 from equine myocardium.the voltage-gated k+-channel kv7.1 and the subunit kcne1, encoded by the kcnq1 and kcne1 genes, respectively, are responsible for termination of the cardiac action potential. in humans, mutations in these genes can predispose patients to arrhythmias and sudden cardiac death (scd).201728917093
organ culture of the xenopus tadpole intestine.during xenopus metamorphosis, most tadpole organs remodel from the larval to adult form to prepare for adaptation to terrestrial life. organ culture serves as an important tool for studying larval-to-adult organ remodeling independent of the effects of other parts of the body. here, i introduce a protocol for organ culture in vitro using the xenopus laevis tadpole intestine before metamorphic climax. during culture in the absence of exogenous 3,3',5-triiodo-l-thyronine (t3), the most potent natu ...201728912375
collagen-embedded tumor transplantations in xenopus laevis tadpoles.the xenopus laevis tadpole provides a valuable model for studying tumorigenesis and tumor immunity by intravital real-time microscopy. using well-characterized thymic lymphoid tumor lines (15/0 and ff-2) that are transplantable into their compatible hosts (lg-15 isogenic clones and the f inbred strain, respectively), a system of semisolid tumor engraftment has been designed. because these lymphoid tumor cell lines are not adherent and grow in suspension, they are first immobilized in a matrix of ...201728912374
models of convergent extension during morphogenesis.convergent extension (ce) is a fundamental and conserved collective cell movement that forms elongated tissues during embryonic development. thus far, studies have demonstrated two different mechanistic models of collective cell movements during ce. the first, termed the crawling mode, was discovered in the process of notochord formation in xenopus laevis embryos, and has been the established model of ce for decades. the second model, known as the contraction mode, was originally reported in stu ...028906063
apetx4, a novel sea anemone toxin and a modulator of the cancer-relevant potassium channel kv10.1.the human ether-à-go-go channel (heag1 or kv10.1) is a cancer-relevant voltage-gated potassium channel that is overexpressed in a majority of human tumors. peptides that are able to selectively inhibit this channel can be lead compounds in the search for new anticancer drugs. here, we report the activity-guided purification and electrophysiological characterization of a novel kv10.1 inhibitor from the sea anemone anthopleura elegantissima. purified sea anemone fractions were screened for inhibit ...201728902151
depolarization of the conductance-voltage relationship in the nav1.5 mutant, e1784k, is due to altered fast inactivation.e1784k is the most common mixed long qt syndrome/brugada syndrome mutant in the cardiac voltage-gated sodium channel nav1.5. e1784k shifts the midpoint of the channel conductance-voltage relationship to more depolarized membrane potentials and accelerates the rate of channel fast inactivation. the depolarizing shift in the midpoint of the conductance curve in e1784k is exacerbated by low extracellular ph. we tested whether the e1784k mutant shifts the channel conductance curve to more depolarize ...201728898267
the effect of 4-hydroxybenzaldehyde on the γ-aminobutyric acid type a receptor.the α1β2γ2 subtype of gabaa receptors is the most commonly found gabaa receptor subtype in the mammalian cortex and hippocampus. it is expressed heterologously in the xenopus laevis oocyte as a α1β2γ2s/l subtype for application as an in vitro model for the screening of compounds that modulate receptor activities. in fact, 4-hydroxybenzaldehyde (4-hb) has been identified as one of the major components in dendrocalamus asper bamboo shoots in our previous study, and the current study showed that at ...201728894409
ultrafast flavin photoreduction in an oxidized animal (6-4) photolyase through an unconventional tryptophan tetrad.photolyases are flavoenzymes repairing uv-induced lesions in dna, which may be activated by a photoreduction of their fad cofactor. in most photolyases, this photoreduction proceeds by electron transfer along a chain of three tryptophan (trp) residues, connecting the flavin to the protein surface. much less studied, animal (6-4) photolyases (repairing pyrimidine-pyrimidone (6-4) photoproducts) are particularly interesting as they were recently shown to have a longer electron transfer chain, coun ...201728890968
sumo co-expression modifies kv 11.1 channel activity.the voltage-gated potassium channel kv 11.1 is the molecular basis for the ikr current, which plays an important role in cardiac physiology. its malfunction is associated with both inherited and acquired cardiac arrhythmias. native currents differ from those in experimental models, suggesting additional regulatory mechanisms. we hypothesized that the post-translational modification sumoylation fine-tunes channel activity.201728888063
interleukin-11 induces and maintains progenitors of different cell lineages during xenopus tadpole tail regeneration.unlike mammals, xenopus laevis tadpoles possess high ability to regenerate their lost organs. in amphibians, the main source of regenerated tissues is lineage-restricted tissue stem cells, but the mechanisms underlying induction, maintenance and differentiation of these stem/progenitor cells in the regenerating organs are poorly understood. we previously reported that interleukin-11 (il-11) is highly expressed in the proliferating cells of regenerating xenopus tadpole tails. here, we show that i ...201728887447
dextmp: deep dive into text for predicting moonlighting proteins.moonlighting proteins (mps) are an important class of proteins that perform more than one independent cellular function. mps are gaining more attention in recent years as they are found to play important roles in various systems including disease developments. mps also have a significant impact in computational function prediction and annotation in databases. currently mps are not labeled as such in biological databases even in cases where multiple distinct functions are known for the proteins. ...201728881966
loss of fourth electron-transferring tryptophan in animal (6-4) photolyase impairs dna repair activity in bacterial cells.(6-4) photolyases [(6-4)pls] are flavoproteins that use blue light to repair the ultraviolet-induced pyrimidine(6-4)pyrimidone photoproduct in dna. their flavin adenine dinucleotide (fad) cofactor can be reduced to its repair-active fadh- form by a photoinduced electron transfer reaction. in animal (6-4)pls, a chain of four trp residues was suggested to be involved in a stepwise transfer of an oxidation hole from the flavin to the surface of the protein. here, we investigated the effect of mutat ...201728880077
methylisothiazolinone toxicity and inhibition of wound healing and regeneration in planaria.methylisothiazolinone (mit) is a common biocide used in cosmetic and industrial settings. studies have demonstrated that mit is a human sensitizer, to the extent that in 2013 mit was named allergen of the year. recently, we showed that mit exposure in xenopus laevis (the african clawed frog) inhibits wound healing and tail regeneration. however, it is unknown whether mit affects these processes in other animals. here, we investigated the effects of mit exposure in planaria-non-parasitic freshwat ...201728869924
in vitro reconstitution of the endoplasmic reticulum.reconstitution of cellular organelles in vitro offers the possibility to perform quantitative and qualitative experiments in a controlled environment that cannot be done with the same accuracy in living cells. following a previous report, the subsequent list of protocols describes how to reconstitute and quantify a tubular er network in vitro based on purified microsomes from culture cells and cytosol from xenopus laevis egg extracts. biological material preparation and reconstitution assays req ...201728862340
toxicity effects of functionalized quantum dots, gold and polystyrene nanoparticles on target aquatic biological models: a review.nano-based products are widespread in several sectors, including textiles, medical-products, cosmetics, paints and plastics. nanosafety and safe-by-design are driving nanoparticle (np) production and applications through np functionalization (@nps). indeed, @nps frequently present biological effects that differ from the parent material. this paper reviews the impact of quantum dots (qds), gold nanoparticles (aunps), and polystyrene-cored nps (psnps), evidencing the role of np functionalization i ...201728858240
advances in the use of xenopus for successful drug screening.understanding embryogenesis currently relies largely on the control of gene expression via several signaling pathways. many of the embryonic signaling pathways guiding embryological events are implicated in diseases that lack effective cure or treatment. because of the large number and size of the eggs, the rapid development of the embryos and the fact they are amenable to pharmacological, surgical and genetic techniques, xenopus laevis has been successfully used in searching for compounds that ...201728846034
dual control of pcdh8l/pcns expression and function in xenopus laevis neural crest cells by adam13/33 via the transcription factors tfap2α and arid3a.adam13/33 is a cell surface metalloprotease critical for cranial neural crest (cnc) cell migration. it can cleave multiple substrates including itself, fibronectin, ephrinb, cadherin-11, pcdh8 and pcdh8l (this work). cleavage of cadherin-11 produces an extracellular fragment that promotes cnc migration. in addition, the adam13 cytoplasmic domain is cleaved by gamma secretase, translocates into the nucleus and regulates multiple genes. here, we show that adam13 interacts with the arid3a/dril1/bri ...201728829038
assembly rules for gabaa receptor complexes in the brain.gabaa receptor (gabaar) pentamers are assembled from a pool of 19 subunits, and variety in subunit combinations diversifies gabaar functions to tune brain activity. pentamers with distinct subunit compositions localize differentially at synaptic and non-synaptic sites to mediate phasic and tonic inhibition, respectively. despite multitudes of theoretical permutations, limited subunit combinations have been identified in the brain. currently, no molecular model exists for combinatorial gabaar ass ...201728816653
the crystal structure of full-length sizzled from xenopus laevis yields insights into wnt-antagonistic function of secreted frizzled-related proteins.the wnt-signaling pathway is crucial to cell proliferation, differentiation, and migration. the secreted frizzled-related proteins (sfrps) represent the largest family of secreted wnt inhibitors. however, their function in antagonizing wnt signaling has remained somewhat controversial. here, we report the crystal structure of sizzled from xenopus laevis, the first full-length structure of an sfrp. tethered by an inter-domain disulfide bond and a linker, the n-terminal cysteine-rich domain (crd) ...201728808056
clathrodin, hymenidin and oroidin, and their synthetic analogues as inhibitors of the voltage-gated potassium channels.we have prepared three alkaloids from the agelas sponges, clathrodin, hymenidin and oroidin, and a series of their synthetic analogues, and evaluated their inhibitory effect against six isoforms of the kv1 subfamily of voltage-gated potassium channels, kv1.1-kv1.6, expressed in chinese hamster ovary (cho) cells using automated patch clamp electrophysiology assay. the most potent inhibitor was the (e)-n-(3-(2-amino-1h-imidazol-4-yl)allyl)-4,5-dichloro-1h-pyrrole-2-carboxamide (6g) with ic50 value ...201728802123
both nuclear size and dna amount contribute to midblastula transition timing in xenopus laevis.during early xenopus laevis embryogenesis both nuclear and cell volumes decrease with the nuclear-to-cytoplasmic (n/c) volume ratio reaching a maximum at the midblastula transition (mbt). at the mbt, embryonic transcription is upregulated and cell cycles lengthen. early studies demonstrated a role for the dna-to-cytoplasmic ratio in the control of mbt timing. by altering nuclear size, we previously showed that the n/c volume ratio also contributes to proper mbt timing. here we examine the relati ...201728801588
role of aqp9 in transport of monomethyselenic acid and selenite.aqp9 is an aquaglyceroporin with a very broad substrate spectrum. in addition to its orthodox nutrient substrates, aqp9 also transports multiple neutral and ionic arsenic species including arsenic trioxide, monomethylarsenous acid (masiii) and dimethylarsenic acid (dmav). here we discovered a new group of aqp9 substrates which includes two clinical relevant selenium species. we showed that aqp9 efficiently transports monomethylselenic acid (msea) with a preference for acidic ph, which has been d ...201728798983
in vivo analysis of the neurovascular niche in the developing xenopus brain.the neurovascular niche is a specialized microenvironment formed by the interactions between neural progenitor cells (npcs) and the vasculature. while it is thought to regulate adult neurogenesis by signaling through vascular-derived soluble cues or contacted-mediated cues, less is known about the neurovascular niche during development. in xenopus laevis tadpole brain, npcs line the ventricle and extend radial processes tipped with endfeet to the vascularized pial surface. using in vivo labeling ...201728795134
comprehensive analyses of hox gene expression in xenopus laevis embryos and adult tissues.from whole genome sequencing of an allotetraploid frog, xenopus laevis, two homeologous sets (l and s) of four hox clusters a through d (hoxa.l/s, hoxb.l/s, hoxc.l/s, and hoxd.l/s) and 13 paralogous groups (pgs) with 76 genes were identified, allowing us to carry out the first comprehensive analyses of hox gene expression in vertebrates. expression of all hox genes during development and in adult tissues was analyzed by rna-sequencing. the expression levels of most hox genes were similar between ...201728791673
the α2β2 isoform combination dominates the astrocytic na+ /k+ -atpase activity and is rendered nonfunctional by the α2.g301r familial hemiplegic migraine type 2-associated mutation.synaptic activity results in transient elevations in extracellular k+ , clearance of which is critical for sustained function of the nervous system. the k+ clearance is, in part, accomplished by the neighboring astrocytes by mechanisms involving the na+ /k+ -atpase. the na+ /k+ -atpase consists of an α and a β subunit, each with several isoforms present in the central nervous system, of which the α2β2 and α2β1 isoform combinations are kinetically geared for astrocytic k+ clearance. while transcr ...201728787093
expression of the aquaglyceroporin hc-9 in a freeze-tolerant amphibian that accumulates glycerol seasonally.as ambient temperatures fall in the autumn, freeze-tolerant cope's gray treefrogs, dryophytes chrysoscelis (formerly hyla chrysoscelis), accumulate glycerol as a cryoprotective agent. we hypothesized that these treefrogs express an ortholog of the mammalian aquaglyceroporin aqp9 and that aqp9 expression is upregulated in the cold to facilitate glycerol transport. we sequenced 1790 bp from cloned cdna that codes for a 315 amino acid protein, hc-9, containing the predicted six transmembrane spanni ...201728784850
hmmr mediates anterior neural tube closure and morphogenesis in the frog xenopus.development of the central nervous system requires orchestration of morphogenetic processes which drive elevation and apposition of the neural folds and their fusion into a neural tube. the newly formed tube gives rise to the brain in anterior regions and continues to develop into the spinal cord posteriorly. conspicuous differences between the anterior and posterior neural tube become visible already during neural tube closure (ntc). planar cell polarity (pcp)-mediated convergent extension (ce) ...201728778799
combining cytotoxicity assessment and xenopus laevis phenotypic abnormality assay as a predictor of nanomaterial safety.the african clawed frog, xenopus laevis, has been used as an efficient pre-clinical screening tool to predict drug safety during the early stages of the drug discovery process. x. laevis is a relatively inexpensive model that can be used in whole organism high-throughput assays whilst maintaining a high degree of homology to the higher vertebrate models often used in scientific research. despite an ever-increasing volume of biomedical nanoparticles (nps) in development, their unique physico-chem ...201728777439
multi-omics analysis sheds light on the evolution and the intracellular lifestyle strategies of spotted fever group rickettsia spp.arthropod-borne rickettsia species are obligate intracellular bacteria which are pathogenic for humans. within this genus, rickettsia slovaca and rickettsia conorii cause frequent and potentially severe infections, whereas rickettsia raoultii and rickettsia massiliae cause rare and milder infections. all four species belong to spotted fever group (sfg) rickettsiae. however, r. slovaca and r. raoultii cause scalp eschar and neck lymphadenopathy (senlat) and are mainly associated with dermacentor ...201728775717
tectal corticotropin-releasing factor (crf) neurons respond to fasting and a reactive stressor in the african clawed frog, xenopus laevis.it is well established that hypothalamic neurons producing the peptide corticotropin-releasing factor (crf) play a key role in stress adaptation, including reduction of food intake when a threat or stressor is present. we have previously reported on the presence of an intrinsic crf signaling system within the optic tectum (ot), a brain area that plays a key role in visually guided prey capture/predator avoidance decisions. to better understand the potential role of tectal crf neurons in regulati ...201728774755
heterologous expression of panax ginseng pgtip1 confers enhanced salt tolerance of soybean cotyledon hairy roots, composite, and whole plants.the panax ginseng tip gene pgtip1 was previously demonstrated to have high water channel activity by its heterologous expression in xenopus laevis oocytes and in yeast; it also plays a significant role in growth of pgtip1-transgenic arabidopsis plants under favorable conditions and has enhanced tolerance toward salt and drought treatment. in this work, we first investigated the physiological effects of heterologous pgtip1 expression in soybean cotyledon hairy roots or composite plants mediated b ...201728769947
iron is a substrate of the plasmodium falciparum chloroquine resistance transporter pfcrt in xenopus oocytes.the chloroquine resistance transporter of the human malaria parasite plasmodium falciparum, pfcrt, is an important determinant of resistance to several quinoline and quinoline-like antimalarial drugs. pfcrt also plays an essential role in the physiology of the parasite during development inside erythrocytes. however, the function of this transporter besides its role in drug resistance is still unclear. using electrophysiological and flux experiments conducted on pfcrtexpressing xenopus laevis oo ...201728768767
the zahn drawings: new illustrations of xenopus embryo and tadpole stages for studies of craniofacial development.the embryos and tadpoles of the frog xenopus are increasingly important subjects for studies of the development of the head and face - studies that are providing novel and crucial insight into the causes and prevention of a suite of devastating birth defects, as well as basic evolutionary and developmental biology. however, many studies are conducted on a range of embryonic stages that are not fully represented in the beloved xenopus resource, nieuwkoop and faber's classic normal table of xenopu ...201728765211
modeling dominant and recessive forms of retinitis pigmentosa by editing three rhodopsin-encoding genes in xenopus laevis using crispr/cas9.the utility of xenopus laevis, a common research subject for developmental biology, retinal physiology, cell biology, and other investigations, has been limited by lack of a robust gene knockout or knock-down technology. here we describe manipulation of the x. laevis genome using crispr/cas9 to model the human disorder retinitis pigmentosa, and to introduce point mutations or exogenous dna sequences. we introduced and characterized in-frame and out-of-frame insertions and deletions in three gene ...201728761125
essential roles of thyroid hormone-regulated hyaluronan/cd44 signaling in adult stem cell development during xenopus laevis intestinal remodeling.in the amphibian intestine during metamorphosis, thyroid hormone (th) induces some larval epithelial cells to dedifferentiate into stem cells, which generate the adult epithelium analogous to the mammalian intestinal epithelium. we have previously shown that the canonical wnt signaling pathway is involved in adult epithelial development in the xenopus laevis intestine. to understand the function of this pathway more precisely, we here focused on cd44, a major wnt target, which has been identifie ...201728758360
smarcal1-mediated fork reversal triggers mre11-dependent degradation of nascent dna in the absence of brca2 and stable rad51 nucleofilaments.brca2 deficiency causes mre11-dependent degradation of nascent dna at stalled forks, leading to cell lethality. to understand the molecular mechanisms underlying this process, we isolated xenopus laevis brca2. we demonstrated that brca2 protein prevents single-stranded dna gap accumulation at replication fork junctions and behind them by promoting rad51 binding to replicating dna. without brca2, forks with persistent gaps are converted by smarcal1 into reversed forks, triggering extensive mre11- ...201728757209
the xenopus tadpole: an in vivo model to screen drugs favoring remyelination.in multiple sclerosis, development of screening tools for remyelination-promoting molecules is timely.201728752787
functional characterization of an aquaporin from a microsporidium, nosema bombycis.microsporidia are a diverse group of eukaryotic organisms, capable of causing parasitic infections in both vertebrates and invertebrates. during the germination process, there is an increase in the osmotic pressure of microsporidian spores. as part of this study, we cloned a homologous aquaporin gene in nosema bombycis, and named it nosema bombycis aquaporin (nbaqp). sequence analysis revealed that the nbaqp contains an open reading frame with a length of 750 bp and encodes a polypeptide of 249 ...201728749993
fetax assay for evaluation of developmental toxicity.the frog embryo teratogenesis assay xenopus (fetax) test is a development toxicity screening test. due to the small amount of compound needed and the capability to study organogenesis in a short period of time (96 h), fetax test constitutes an efficient development toxicity alert test when performed early in drug safety development. the test is conducted on fertilized xenopus laevis mid-blastula-stage eggs over the organogenesis period. compound teratogenic potential is determined after analysis ...201728748472
lethal digenic mutations in the k+ channels kir4.1 (kcnj10) and slack (kcnt1) associated with severe-disabling seizures and neurodevelopmental delay.a 2-yr-old boy presented profound developmental delay, failure to thrive, ataxia, hypotonia, and tonic-clonic seizures that caused the death of the patient. targeted and whole exome sequencing revealed two heterozygous missense variants: a novel mutation in the kcnj10 gene that encodes for the inward-rectifying k+ channel kir4.1 and another previously characterized mutation in kcnt1 that encodes for the na+-activated k+ channel known as slo2.2 or slack. the objectives of this study were to perfo ...201728747464
first parasitological study of the african clawed frog (xenopus laevis, amphibia) in chile.introduced species can arrive into new territories with parasites; however, these species are expected to face lower parasite richness than in their original regions. both introduced hosts and parasites can affect native fauna. since their release into the wild in chile following laboratory use, xenopus laevis daudin, 1802 has widely spread throughout central chile. the only pathogen described on the host is the fungus batrachochytrium dendrobatidis longcore, pessier, nichols, 1999; thus, this i ...201728746452
tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in arabidopsis.a great proportion of nitrate taken up by plants is stored in vacuoles. vacuolar nitrate accumulation and release is of great importance to nitrate reallocation and efficient utilization. however, how plants mediate nitrate efflux from vacuoles to cytoplasm is largely unknown. the current study identified npf5.11, npf5.12 and npf5.16 as vacuolar nitrate efflux transporters in arabidopsis. histochemical analysis showed that npf5.11, npf5.12 and npf5.16 were expressed preferentially in root pericy ...201728743909
xenopus laevis m18bp1 directly binds existing cenp-a nucleosomes to promote centromeric chromatin assembly.vertebrate centromeres are epigenetically defined by nucleosomes containing the histone h3 variant, cenp-a. cenp-a nucleosome assembly requires the three-protein mis18 complex (mis18α, mis18β, and m18bp1) that recruits the cenp-a chaperone hjurp to centromeres, but how the mis18 complex recognizes centromeric chromatin is unknown. using xenopus egg extract, we show that direct, cell-cycle-regulated binding of m18bp1 to cenp-a nucleosomes recruits the mis18 complex to interphase centromeres to pr ...201728743005
histone methyltransferase dot1l is a coactivator for thyroid hormone receptor during xenopus development.histone modifications are associated with transcriptional regulation by diverse transcription factors. genome-wide correlation studies have revealed that histone activation marks and repression marks are associated with activated and repressed gene expression, respectively. among the histone activation marks is histone h3 k79 methylation, which is carried out by only a single methyltransferase, disruptor of telomeric silencing-1-like (dot1l). we have been studying thyroid hormone (t3)-dependent ...201728739643
splice variants of ph-sensitive chloride channel identify a key determinant of ivermectin sensitivity in the larvae of the silkworm bombyx mori.the ph-sensitive chloride channels (phcls) are broadly expressed in insects, but little is known about their physiologic role, diversity, and sensitivity to insecticides acting on relevant chloride channels. here we have sequenced 50 transcripts of the phcl-1 gene from the brain, third thoracic ganglion (t3g), and midgut of larvae of silkworm bombyx mori it was found that >50 variants were expressed with distinct splicing in the t3g compared with the brain and midgut. of the variants detected, v ...201728739571
a g326e substitution in the glutamate-gated chloride channel 3 (glucl3) of the two-spotted spider mite tetranychus urticae abolishes the agonistic activity of macrocyclic lactones.the macrocyclic lactones abamectin and milbemectin are frequently used to control phytophagous mites such as tetranychus urticae. consequently, resistance has developed and was genetically linked with substitutions in the glutamate-gated chloride channel (glucl) subunits tuglucl1 and tuglucl3. here, we functionally validated a g326e substitution in tuglucl3 by functional expression in xenopus laevis oocytes followed by two-electrode voltage-clamp electrophysiology.201728736919
neuronal degeneration and regeneration induced by axotomy in the olfactory epithelium of xenopus laevis.the olfactory epithelium (oe) has the remarkable capability to constantly replace olfactory receptor neurons (orns) due to the presence of neural stem cells (nscs). for this reason, the oe provides an excellent model to study neurogenesis and neuronal differentiation. in the present work, we induced neuronal degeneration in the oe of xenopus laevis larvae by bilateral axotomy of the olfactory nerves. we found that axotomy induces specific- neuronal death through apoptosis between 24 and 48h post ...201728719101
conserved gene regulatory module specifies lateral neural borders across bilaterians.the lateral neural plate border (npb), the neural part of the vertebrate neural border, is composed of central nervous system (cns) progenitors and peripheral nervous system (pns) progenitors. in invertebrates, pns progenitors are also juxtaposed to the lateral boundary of the cns. whether there are conserved molecular mechanisms determining vertebrate and invertebrate lateral neural borders remains unclear. using single-cell-resolution gene-expression profiling and genetic analysis, we present ...201728716930
cip29 is phosphorylated following activation of the dna damage response in xenopus egg extracts.acting through a complex signalling network, dna lesions trigger a range of cellular responses including dna repair, cell cycle arrest, altered gene expression and cell death, which help to limit the mutagenic effects of such dna damage. rna processing factors are increasingly being recognised as important targets of dna damage signalling, with roles in the regulation of gene expression and also more directly in the promotion of dna repair. in this study, we have used a xenopus laevis egg extrac ...201728715428
fluorescence correlation spectroscopy experiments to quantify free diffusion coefficients in reaction-diffusion systems: the case of ca^{2+} and its dyes.many cell signaling pathways involve the diffusion of messengers that bind and unbind to and from intracellular components. quantifying their net transport rate under different conditions then requires having separate estimates of their free diffusion coefficient and binding or unbinding rates. in this paper, we show how performing sets of fluorescence correlation spectroscopy (fcs) experiments under different conditions, it is possible to quantify free diffusion coefficients and on and off rate ...201728709293
regulation of the insulin-akt signaling pathway and glycolysis during dehydration stress in the african clawed frog xenopus laevis.estivation is an adaptive stress response utilized by some amphibians during periods of drought in the summer season. in this study, we examine the regulation of the insulin signaling cascade and glycolysis pathway in the african clawed frog xenopus laevis during the dehydration stress induced state of estivation. we show that in the brain and heart of x. laevis, dehydration reduces the phosphorylation of the insulin growth factor-1 receptor (igf-1r), and this is followed by similar reductions i ...201728708941
g-quadruplex dna and ligand interaction in living cells using nmr spectroscopy.gathering structural information from biologically relevant molecules inside living cells has always been a challenging task. in this work, we have used multidimensional nmr spectroscopy to probe dna g-quadruplexes inside living xenopus laevis oocytes. some of these structures can be found in key regions of chromosomes. g-quadruplexes are considered potential anticancer therapeutic targets and several lines of evidence indirectly point out roles in key biological processes, such as cell prolifer ...201528706695
coordinating heart morphogenesis: a novel role for hyperpolarization-activated cyclic nucleotide-gated (hcn) channels during cardiogenesis in xenopus laevis.hyperpolarization-activated cyclic-nucleotide gated channel (hcn) proteins are important regulators of both neuronal and cardiac excitability. among the 4 hcn isoforms, hcn4 is known as a pacemaker channel, because it helps control the periodicity of contractions in vertebrate hearts. although the physiological role of hcn4 channel has been studied in adult mammalian hearts, an earlier role during embryogenesis has not been clearly established. here, we probe the embryonic roles of hcn4 channels ...201728702127
charged antimicrobial peptides can translocate across membranes without forming channel-like pores.how can highly charged, cationic antimicrobial peptides (amps) translocate across hydrophobic lipid bilayers despite the prohibitive energetic penalty to do so? a common explanation has been the formation of peptide-lined channels. however, for most amps, no structures of membrane pores have been found despite clear evidence of membrane leakage and antimicrobial activity. the study here suggests an alternative and simple reason: for the amp pgla from xenopus laevis (charge +5), such pores are no ...201728700927
chk1 inhibition of the replication factor drf1 guarantees cell-cycle elongation at the xenopus laevis mid-blastula transition.the early cell divisions of many metazoan embryos are rapid and occur in the near absence of transcription. at the mid-blastula transition (mbt), the cell cycle elongates and several processes become established including the onset of bulk transcription and cell-cycle checkpoints. how these events are timed and coordinated is poorly understood. here we show in xenopus laevis that developmental activation of the checkpoint kinase chk1 at the mbt results in the scfβ-trcp-dependent degradation of a ...201728697335
apolipoprotein c-i mediates wnt/ctnnb1 signaling during neural border formation and is required for neural crest development.in vertebrates, the neural crest and placodes originate in the neural border, which is located between the neural plate and epidermal ectoderm. the neural crest and placodes give rise to a vast array of cell types. formation of neural crest is a multi-step process, in which wnt signals are used reiteratively, but it is currently not clear if a wnt signal is required for neural border formation. here, we have identified apolipoprotein c-i (apoc1) in a screen for genes regulated by wnt/ctnnb1 sign ...201728695961
the effect of pungent and tingling compounds from piper nigrum l. on background k+ currents.black peppercorns (piper nigrum l.) elicit a pungent and tingling oral impression. their pungency is partially explained by the agonist activity of some of their active principles, especially piperine, on trp channels. however, we recently showed that piperine, as well as other pungent compounds, also possess a marked effect on two-pore domain (kcnk, k2p) k+ channels. members of this family play a key role in maintaining the resting membrane potential of excitable cells. interestingly, tingling ...201728694780
high-efficiency non-mosaic crispr-mediated knock-in and indel mutation in f0 xenopus.the revolution in crispr-mediated genome editing has enabled the mutation and insertion of virtually any dna sequence, particularly in cell culture where selection can be used to recover relatively rare homologous recombination events. the efficient use of this technology in animal models still presents a number of challenges, including the time to establish mutant lines, mosaic gene editing in founder animals, and low homologous recombination rates. here we report a method for crispr-mediated g ...201728694259
a functional approach to understanding the role of nckx5 in xenopus pigmentation.nckx5 is an ion exchanger expressed mostly in pigment cells; however, the functional role for this protein in melanogenesis is not clear. a variant allele of slc24a5, the gene encoding nckx5, has been shown to correlate with lighter skin pigmentation in humans, indicating a key role for slc24a5 in determining human skin colour. slc24a5 expression has been found to be elevated in melanoma. knockdown analyses have shown slc24a5 to be important for pigmentation, but to date the function of this ion ...201728692664
family of ricinus communis monosaccharide transporters and rcstp1 in promoting the uptake of a glucose-fipronil conjugate.enhancing the systemic distribution of a bioactive compound by exploiting the vascular transport system of a plant presents a means of reducing both the volume and frequency of pesticide/fungicide application. the foliar uptake of the glucose-fipronil conjugate n-[3-cyano-1-[2,6-dichloro-4-(trifluoromethyl)phenyl]-4-[(trifluoromethyl)sulfinyl]-1h-pyrazol-5-yl]-1-(β-d-glucopyranosyl)-1h-1,2,3-triazole-4-methanamine (gtf) achieved in castor bean (ricinus communis) and its transport via the phloem ...201728692262
the cannabinoid receptor interacting proteins 1 of zebrafish are not required for morphological development, viability or fertility.the cannabinoid receptor interacting protein 1 (cnrip1) was discovered as an interactor with the intracellular region of cannabinoid receptor 1 (cb1r, also known as cnr1 or cb1). functional assays in mouse show cannabinoid sensitivity changes and cnrip1 has recently been suggested to control eye development in xenopus laevis. two cnrip1 genes are described in zebrafish, cnrip1a and cnrip1b. in situ mrna hybridisation revealed accumulation of mrna encoding each gene primarily in brain and spinal ...201728687732
acetylcholinesterase plays a non-neuronal, non-esterase role in organogenesis.acetylcholinesterase (ache) is crucial for degrading acetylcholine at cholinergic synapses. in vitro studies suggest that, in addition to its role in nervous system signaling, ache can also modulate non-neuronal cell properties, although it remains controversial whether ache functions in this capacity in vivo here, we show that ache plays an essential non-classical role in vertebrate gut morphogenesis. exposure of xenopus embryos to ache-inhibiting chemicals results in severe defects in intestin ...201728684626
the regulation of heat shock proteins in response to dehydration in xenopus laevis.african clawed frogs (xenopus laevis) endure bouts of severe drought in their natural habitats and survive the loss of approximately 30% of total body water due to dehydration. to investigate molecular mechanisms employed by x. laevis during periods of dehydration, the heat shock protein response, a vital component of the cytoprotective stress response, was characterized. using western immunoblotting and multiplex technology, the protein levels of hsp27, hsp40, hsp60, hsp70, hsc70, and hsp90 wer ...028676984
maitotoxin is a potential selective activator of the endogenous transient receptor potential canonical type 1 channel in xenopus laevis oocytes.maitotoxin (mtx) is the most potent marine toxin known to date. it is responsible for a particular human intoxication syndrome called ciguatera fish poisoning (cfp). several reports indicate that mtx is an activator of non-selective cation channels (nscc) in different cell types. the molecular identity of these channels is still an unresolved topic, and it has been proposed that the transient receptor potential (trp) channels are involved in this effect. in xenopus laevis oocytes, mtx at picomol ...201728672825
the tyrosine y2502.39 in frizzled 4 defines a conserved motif important for structural integrity of the receptor and recruitment of disheveled.frizzleds (fzds) are unconventional g protein-coupled receptors, which activate diverse intracellular signaling pathways via the phosphoprotein disheveled (dvl) and heterotrimeric g proteins. the interaction interplay of fzds with dvl and g proteins is complex, involves different regions of fzd and the potential dynamics are poorly understood. in the present study, we aimed to characterize the function of a highly conserved tyrosine (y2502.39) in the intracellular loop 1 (il1) of human fzd4. we ...201728668722
developmental neurogenesis in mouse and xenopus is impaired in the absence of nosip.genetic deletion of nosip in mice causes holoprosencephaly, however, the function of nosip in neurogenesis is currently unknown.201728663132
a multi-tiered, in vivo, quantitative assay suite for environmental disruptors of thyroid hormone signaling.the essential role of thyroid hormone (th) signaling in mammalian development warrants the examination of man-made chemicals for its disruption. among vertebrate species, the molecular components of th signaling are highly conserved, including the thyroid hormone receptors (trs), their heterodimer binding partners the retinoid-x receptors (rxrs), and their dna recognition sequences (tres). this molecular conservation allows examination of potential th disruption in the tractable, in vivo model s ...201728662416
renal localization and regulation by dietary phosphate of the mct14 orphan transporter.mct14 is an orphan transporter belonging to the slc16 transporter family mediating the transport of monocarboxylates, aromatic amino acids, creatine, and thyroid hormones. the expression, tissue localization, regulation, and function of mct14 are unknown. in mouse mct14 mrna abundance is highest in kidney. using a newly developed and validated antibody, mct14 was localized to the luminal membrane of the thick ascending limb of the loop of henle colocalizing in the same cells with uromodulin and ...201728662032
a method for detailed movement pattern analysis of tadpole startle response.prolonged space flight, specifically microgravity, presents a problem for space exploration. animal models with altered connections of the vestibular ear, and thus altered gravity sensation, would allow the examination of the effects of microgravity and how various countermeasures can establish normal function. we describe an experimental apparatus to monitor the effects of ear manipulations to generate asymmetric gravity input on the tadpole escape response. to perform the movement pattern anal ...201728653338
switch-like behavior enables wnt11 concentration specific response during dorso-ventral axis formation in xenopus laevis.wnt signaling plays a role in diverse processes such as cell proliferation, differentiation, migration or cell polarity. dysfunction of wnt signaling is associated with human diseases and aging. wnts can activate several interacting intracellular signaling pathways, in particular the so called canonical and non-canonical pathways. the canonical wnt response leads to a stabilization of cytoplasmic β-catenin whereas non-canonical wnt signaling can result in the activation of calcium-calmodulin dep ...201728648560
ensemble and single-molecule analysis of non-homologous end joining in frog egg extracts.non-homologous end joining (nhej) repairs the majority of dna double-strand breaks in human cells, yet the detailed order of events in this process has remained obscure. here, we describe how to employ xenopus laevis egg extract for the study of nhej. the egg extract is easy to prepare in large quantities, and it performs efficient end joining that requires the core end joining proteins ku, dna-pkcs, xlf, xrcc4, and dna ligase iv. these factors, along with the rest of the soluble proteome, are p ...201728645371
xenopus laevis as model system to study dna damage response and replication fork stability.although many players of the dna damage response and dna repair have been identified in several systems their biochemical role is still poorly understood. the use of the xenopus laevis egg extract cell-free system allowed biochemical dissection of dna replication and cell cycle events in a complex biological context. the possibility of manipulating the protein content by using protein depletion procedures makes egg extract a powerful system to study proteins whose inactivation results in cellula ...201728645370
adipose tissue macrophages develop from bone marrow-independent progenitors in xenopus laevis and mouse.atms have a metabolic impact in mammals as they contribute to metabolically harmful at inflammation. the control of the atm number may have therapeutic potential; however, information on atm ontogeny is scarce. whereas it is thought that atms develop from circulating monocytes, various tissue-resident mϕs are capable of self-renewal and develop from bm-independent progenitors without a monocyte intermediate. here, we show that amphibian at contains self-renewing atms that populate the at before ...201728642277
using short-term bioassays to evaluate the endocrine disrupting capacity of the pesticides linuron and fenoxycarb.several short-term whole-organism bioassays based on transgenic aquatic models are now under validation by the oecd (organization for economic co-operation and development) to become standardized test guidelines for the evaluation of the endocrine activity of substances. evaluation of the endocrine disrupting capacity of pesticides will be a domain of applicability of these future reference tests. the herbicide linuron and the insecticide fenoxycarb are two chemicals commonly used in agricultura ...201728634053
comparative micrornaome analysis of the testis and ovary of the chinese giant salamander.micrornas (mirnas) are 18-24 nucleotides non-coding rnas that regulate gene expression by post-transcriptional suppression of mrna. the chinese giant salamander (cgs, andrias davidianus), which is an endangered species, has become one of the important models of animal evolution; however, no mirna studies on this species have been conducted. in this study, two small rna libraries of cgs ovary and testis were constructed using deep sequencing technology. a bioinformatics pipeline was developed to ...201728630098
measurement of surface topography and stiffness distribution on cross-section of xenopus laevis tailbud for estimation of mechanical environment in embryo.the stress distribution inside a xenopus laevis tailbud embryo was estimated to examine the cause of the straightening and elongation. the embryos were cut in the middle, yielding a cross-section perpendicular to the body axis. the section was not flat, owing to the residual stress relief. the stress needed to restore the flatness corresponded to the stress inside the embryo and was calculated using the surface topography and young's-moduli in the section. we found the areas of the notochord (nc ...201728627765
xenopus-fv3 host-pathogen interactions and immune evasion.we first review fundamental insights into anti-ranavirus immunity learned with the xenopus laevis/ranavirus fv3 model that are generally applicable to ectothermic vertebrates. we then further investigate fv3 genes involved in immune evasion. focusing on fv3 knockout (ko) mutants defective for a putative viral caspase activation and recruitment domain-containing (card)-like protein (δ64r-fv3), a β-hydroxysteroid dehydrogenase homolog (δ52l-fv3), and an immediate-early18kda protein (fv3-δ18k), we ...201728625407
mutation of the inhibitory ethanol site in gabaa ρ1 receptors promotes tolerance to ethanol-induced motor incoordination.genes encoding the ρ1/2 subunits of gabaa receptors have been associated with alcohol (ethanol) dependence in humans, and ρ1 was also shown to regulate some of the behavioral effects of ethanol in animal models. ethanol inhibits gaba-mediated responses in wild-type (wt) ρ1, but not ρ1(t6'y) mutant receptors expressed in xenopus laevis oocytes, indicating the presence of an inhibitory site for ethanol in the second transmembrane helix. in this study, we found that ρ1(t6'y) receptors expressed in ...201728623169
adhfe1: a novel enzyme involved in retinoic acid-dependent hox activation.retinoic acid (ra) signaling is a central pathway regulating anterior-posterior patterning of the embryo through its targets, the hox genes. ra is produced by two sequential oxidations from vitamin a (retinol) and this biosynthesis has to be regulated temporally, spatially and quantitatively. mining xenopus embryonic expression databases identified a novel component of the ra metabolic network, adhfe1. using xenopus laevis embryos as our experimental system we determined the temporal and spatial ...201728621427
foxd1 protein interacts with wnt and bmp signaling to differentially pattern mesoderm and neural tissue.the foxd1 gene (previously known as brain factor 2/bf2) is expressed during early xenopus laevis development. at gastrula stages, foxd1 is expressed in dorsal mesoderm regions fated for muscle and notochord, while at neurula stages, foxd1 is expressed in the forebrain region. previous studies in the neural plate showed that foxd1 protein acts as transcriptional repressor downstream of bmp antagonism, neuralizing the embryo to control anterior neural cell fates. foxd1 mesoderm function was not ri ...201728621426
roles of the cilium-associated gene ccdc11 in left-right patterning and in laterality disorders in humans.axial determination occurs during early stages of embryogenesis. flaws in laterality patterning result in abnormal positioning of visceral organs, as manifested in heterotaxy syndrome, or complete left-right inversion as in situs inversus totalis. these malformations are often associated with ciliopathies, as seen in primary ciliary dyskinesia. we have recently described a novel mutation in the coiled-coil domain-containing 11 (ccdc11) gene associated with laterality disorders in a consanguineou ...201728621423
cells from subcutaneous tissues contribute to scarless skin regeneration in xenopus laevis froglets.mammals cannot regenerate the dermis and other skin structures after an injury and instead form a scar. however, a xenopus laevis froglet can regenerate scarless skin, including the dermis and secretion glands, on the limbs and trunk after skin excision. subcutaneous tissues in the limbs and trunk consist mostly of muscles. although subcutaneous tissues beneath a skin injury appear disorganized, the cellular contribution of these underlying tissues to skin regeneration remains unclear.201728618059
loops d, e and g in the drosophila dα1 subunit contribute to high neonicotinoid sensitivity of dα1-chicken β2 nicotinic acetylcholine receptor.neonicotinoid insecticides interact with the orthosteric site formed at subunit interfaces of insect nicotinic ach (nach) receptors. however, their interactions with the orthosteric sites at α-non α and α-α subunit interfaces remain poorly understood. the aim of this study was to elucidate the mechanism of neonicotinoid actions using the drosophila dα1-chicken β2 hybrid nach receptor.201728616862
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