Publications

[study of amphibian brain asymmetry during normal embryonic and larval development].	amphibian embryos (triturus vulgaris, pleurodeles waltl, hynobius keyserlingii, ambystoma mexicanum, rana temporaria, bufo bufo, and xenopus laevis) were investigated. we ascertained the morphological right-side asymmetry of the brain, which appears at the neurula stage and is preserved during the postembryonic (larval) development. in t. vulgaris, p. waltl, h. keyserlingii, and x. laevis, we observed right-side asymmetry of peripheral analyzers, such as the retina, ganglia of nerve viii, and ol ...	1998	9644914
lectin binding patterns in amphibian skin epithelium.	seven lectins (pna, dba, wga, uea-i, rca, sba, con a) were used to localize glycoconjugates in the skin of 10 species of amphibia, 7 anurans (bufo marinus, bufo bufo, rana ridibunda, rana pipiens, hyla arborea, pelobates syriacus and xenopus laevis) and 3 urodeles (salamandra salamandra, triturus vulgaris and ambystoma mexicanum). it was found that every lectin has a specific binding pattern in the skin of each species. no common pattern could be established, either among frogs or toads, nor for ...	1999	10443294
sperm storage in females of the smooth newt (triturus v. vulgaris l.): i. ultrastructure of the spermathecae during the breeding season.	sperm storage in cloacal spermathecae was studied in females of triturus v. vulgaris collected early in the breeding season in southern england. females collected in terrestrial situations, presumably unmated, were mated in the laboratory, and the ultrastructure of the transferred sperm and the spermathecae was observed at various intervals after mating. sperm from a spermatophore cap lodged in a female's cloacal orifice can migrate into spermathecae within 1 hr after mating. spherical structure ...	1999	9990737
myotomal myogenesis in triturus vulgaris l. (urodela) with special reference to the role of mesenchymal cells.	two stages can be distinguished in the differentiation of myotomal muscle fibres in triturus vulgaris. in the first stage only synchronously differentiating myotomal cells are engaged; in the second stage mesenchymal cells also take part in the process. myotomal cells (primary myoblasts) fuse to form 2-3 nucleate myotubes. only in the caudal part of the embryo mononucleate myotubes persist. the mononucleate myotubes, like polynucleate ones, occupy the whole length of the myotome. the differentia ...	2000	11080917
paedogenesis in european newts (triturus: salamandridae): cranial morphology during ontogeny.	a cross-sectional analysis using different ontogenetic stages (larvae, juveniles, paedotypic, and metamorphic adults) of the smooth newt, triturus vulgaris, and the alpine newt, t.alpestris, revealed a broad spectrum of perennibranchiation influences on cranial ontogeny in european newts, more pronounced than previously thought. these influences included marked variation in ossification levels, pronounced morphometric variability of many cranial elements, and considerable skull shape changes in ...	2000	10658197
genetic differentiation of the two subspecies of the smooth newt inhabiting romania, triturus vulgaris vulgaris and t.v. ampelensis (urodela, salamandridae) as revealed by enzyme electrophoresis.	starch gel electrophoresis was applied to quantify genetic variation and divergence in samples from romanian populations of the nominal form of the smooth newt triturus vulgaris and those of the endemic romanian subspecies t.v. ampelensis, a population from a parapatric area was additionally included. all the samples had similar levels of genetic variation measured by the mean heterozygosity, proportion of polymorphic loci, and mean number of alleles per locus. t.v. ampelensis samples were genet ...	2001	11987463
distribution of blue-sensitive photoreceptors in amphibian retinas.	previously, we reported that an opsin (rc-ms) belonging to the sws2 group opsins is expressed in bullfrog green rods [hisatomi, o. et al., febs lett., 1999, 447, 44-48]. an anti-rc-ms antiserum recognized the cones of the japanese common newt, cynops pyrrhogaster, which has no green rods. we isolated a cdna encoding an sws2 group opsin (cp-sws2) from this newt and found that cp-sws2 is expressed in a small population of the cones. our results suggest that sws2 opsins can be expressed in either g ...	2001	11470275
opsin expression in adult, developing, and regenerating newt retinas.	japanese common newts (cynops pyrrhogaster) have an ability to regenerate their neural retina even as adults. although extensive research has been carried out attempting to understand this retinal regeneration, the molecules characterized in newt retina are limited. we isolated cdnas encoding three putative opsins (cp-rh, -lws and -sws1), in addition to cp-sws2 [takahashi et al., febs lett. 501 (2001) 151-155] from a cdna library of adult newt retina. our immunohistochemical and in situ hybridiz ...	2002	12106689
display lateralisation in the courtship behaviour of the alpine newt (triturus alpestris).	among urodelan amphibians, european newts of the genus triturus have evolved a complex sequence of displays to the effect of stimulating the female to actively pick up the spermatophore deposited by the male. courtship is varied, with plenty of potential for asymmetric uses of tails and of asymmetric turns. the analysis of the temporal structure of the behavioural sequences showed that both males and females significantly utilise the same side during a single courtship sequence. moreover, if an ...	2002	15513203
molecular basis of spectral tuning in the newt short wavelength sensitive visual pigment.	previously we reported the sequence of the member of the short wavelength sensitive 2 (sws2) family of vertebrate visual pigments from the retina of the japanese common newt, cynops pyrrhogaster[takahashi, y. et al. (2001) febs lett. 501, 151-155]. now we have expressed the apopigment and regenerated it with a1 retinal. its absorption maximum, 474 nm, is greatly red shifted compared to other known sws2 pigments (418-455 nm). to determine the amino acid residues that control its spectral tuning, ...	2003	12755604
nuclear markers, mitochondrial dna and male secondary sexual traits variation in a newt hybrid zone (triturus vulgaris x t. montandoni).	the smooth and the montandon's newts (triturus vulgaris and t. montandoni) are genetically similar sister species with highly divergent male secondary sexual traits involved in complex courtship behaviour. their parapatric ranges overlap at moderate elevations in the carpathian mountains where they hybridize readily. here we present a detailed study of genetic and morphological variation in populations from the area of sympatry. analysis of variation at seven nuclear markers, mtdna and male sexu ...	2003	12803641
expression of fgf2 in the limb blastema of two salamandridae correlates with their regenerative capability.	limb regenerative potential in urodeles seems to vary among different species. we observed that triturus vulgaris meridionalis regenerate their limbs significantly faster than t. carnifex, where a long gap between the time of amputation and blastema formation occurs, and tried to identify cellular and molecular events that may underlie these differences in regenerative capability. whereas wound healing is comparable in the two species, formation of an apical epidermal cap (aec), which is require ...	2003	14613605
phylogeography of two european newt species--discordance between mtdna and morphology.	the newts triturus vulgaris and triturus montandoni are sister species that exhibit contrasting levels of intraspecific morphological variation. triturus vulgaris has a broad eurasiatic distribution encompassing both formerly glaciated and unglaciated areas and shows substantial morphological differentiation in the southern part of its range, while t. montandoni, confined to the carpathians, is morphologically uniform. we analysed sequence variation of two mtdna fragments of the total length of ...	2005	15969729
downregulation of otx2 in the dedifferentiated rpe cells of regenerating newt retina.	cynops pyrrhogaster (the japanese common newt) regenerates neural retina from retinal pigmented epithelium (rpe) cells. otx2 is a transcription factor that is involved in rpe cell differentiation. to understand the role of otx2 during transdifferentiation of rpe cells, we cloned a cynops otx2 cdna, and explored its expression by rt-pcr, immunohistochemistry and in situ hybridization. the expression of otx2 was compared with the localization of a proliferating cell marker (pcna), rpe cell markers ...	2005	15763275
ultrastructural observations on blood vessels surrounding normal and regenerating spinal cord in newt.	the ultrastructural analysis of capillaries surrounding normal and regenerating caudal spinal cords of two species of newt (triturus vulgaris and t. cristatus) is reported. around normal spinal cords, capillaries were generally continuous. surrounding the regenerating spinal cords the various capillaries of the regenerating blastema appeared discontinuous with small or broad gaps along the capillary wall. this was seen even after two months of tail regeneration when the spinal cord was similar t ...	2006	1303068
[relative characteristics of the duration of embryonic development in triturus vulgaris].	the data of valouch et al. (1971) on tau 0 values in the smooth newt at different temperatures are presented as a curve. using this curve, the absolute (in hours and days) duration of successive developmental stages provided by glaesner (1925) is transformed in a relative one, expressed in the number of tau 0 (tau n/tau 0).	2006	6739036
a molecular assessment of phylogenetic relationships and lineage accumulation rates within the family salamandridae (amphibia, caudata).	we examine phylogenetic relationships among salamanders of the family salamandridae using approximately 2700 bases of new mtdna sequence data (the trnaleu, nd1, trnaile, trnagln, trnamet, nd2, trnatrp, trnaala, trnaasn, trnacys, trnatyr, and coi genes and the origin for light-strand replication) collected from 96 individuals representing 61 of the 66 recognized salamandrid species and outgroups. phylogenetic analyses using maximum parsimony and bayesian analysis are performed on the new data alo ...	2006	16815049
[first research on larval parabiosis of f1 hybrids (triturus cristatus male times triturus vulgaris female) with the paternal species].		2006	14801217
genetic dissimilarity predicts paternity in the smooth newt (lissotriton vulgaris).	under sperm competition, paternity is apportioned by polyandrous females according to the order of matings and the genetic quality of the inseminating males. in order to distinguish between these two effects, we sequentially paired 12 female smooth newts (lissotriton vulgaris) with each of two males and, where possible, repeated the same procedure in reverse order of the identical males after assumed sperm depletion. for a total of 578 offspring, amplified fragment length polymorphisms genetic m ...	2007	17638673
stathmin expression during newt retina regeneration.	japanese common newts (cynops pyrrhogaster) have high ability to regenerate their injured organs including neural tissues, for example, the neural retina belonging to central nervous system. we attempted to clarify the molecular mechanism underlying the formation of a neural network during newt retina regeneration, and focused on the microtubule dynamics controlled by stathmin family proteins. stathmin is a small cytoplasmic phosphoprotein known to be a microtubule regulator. we isolated a clone ...	2007	17707372
amphibian occurrence is influenced by current and historic landscape characteristics.	human-induced habitat loss and degradation are major threats to wetland species as reflected in the fact that wetlands have declined by more than 50% in europe and north america during the last century. both current and historic land-use patterns are likely to be significant determinants of wetland species' distributions; however their relative importance is often unknown. we studied the importance of local (study pond) and landscape (current and 18th-century landscape) characteristics in explai ...	2007	18213970
tetrodotoxin and its analogue 6-epitetrodotoxin in newts (triturus spp.; urodela, salamandridae) from southern germany.	tetrodotoxin (ttx) and its analogue 6-epitetrodotoxin (6-epittx) were quantitatively assayed in 59 newts representing four triturus species (triturus alpestris, triturus cristatus, triturus helveticus, triturus vulgaris) from southern germany by a post-column fluorescent-hplc system. both toxins were detected in only 15 specimens of the four species. the toxins levels varied considerably among individuals (ttx: 0.11-9.0microg/g; 6-epittx: 0.05-17.0microg/g). 6-epittx was found to be the major co ...	2007	17507070
[quantitative evaluation of the genetic divergence of amphibian taxa of different rank by the spectra of total proteins and nonspecific esterases].	based on the paag disc-electrophoretic spectra of water-soluble total proteins (pr) and non-specific esterases (est) from several types of tissues in representatives of urodela (triturus vulgaris vulgaris, t. v. lantzi, t. cristatus, t. montandoni, t. dobrogicus) and anura amphibians (rana ridibunda, r. lessonae, r. temporaria, r. arvalis; bufo bufo, b. viridis, bombina bombina; xenopus laevis, x. borealis; hymenochirus boettgeri), mean genetic distances (d = -ln[2i/(2i + d)], where i is the num ...	2007	17484154
a record of gravid cestodes bothriocephalus claviceps (goeze, 1782) from the newts triturus vulgaris (l.) (amphibia).		2007	2227717
[the determination of ectodermal derivatives in various species of amphibia].	the process of labyrinth determination has been studied in amphibians (three urodela and seven anura species) using homoplastic transplantation of ear ectoderm, containing labyrinth material onto the abdominal wall of embryos of the same stage of development. the stage of appearance of organ-specific properties in ear ectoderm was determined and the increase of these properties in the course of development was observed. the frequency of ear vesicles formation, the level of their differentiation ...	2008	2717137
multiple sex pheromone genes are expressed in the abdominal glands of the smooth newt (lissotriton vulgaris) and montandon's newt (l. montandoni) (salamandridae).	the smooth newt (lissotriton "triturus" vulgaris) and montandon's newt (l."t." montandoni) are sister species exhibiting pronounced differences in male secondary sexual traits but nevertheless hybridizing and producing fertile hybrids in nature. since pheromonal communication is an important aspect of the reproductive biology of urodeles, structural differentiation of peptide pheromones and their receptors may contribute to incipient reproductive isolation. the aim of the study was the identific ...	2008	18624569
a case of reproductive character displacement in female palmate newts (lissotriton helveticus).	reproductive character displacement is commonly described as larger phenotypic differences between species living in sympatry rather than in allopatry. we investigated this phenomenon on two amphibians found in their contact zone where syntopic and allotopic sites alternate. to test the effect of syntopy with lissotriton vulgaris on the lissotriton helveticus phenotype, we studied the morphology of adult males and females while controlling for environmental factors (i.e. land use and flood risk) ...	2009	19520318
new polymorphic microsatellite loci for the greek smooth newt, lissotriton vulgaris graecus, and their utility in the nominotypical subspecies.	eight polymorphic microsatellites were isolated from the greek smooth newt (lissotriton vulgaris graecus) using a microsatellite enrichment protocol and selective hybridization with a biotinylated (ac)(11) probe. the loci showed different variation patterns in a single breeding population (32 individuals) with mean number of alleles at 5.0 and mean observed heterozygosity at 0.520. the amplification success also in the nominotypical subspecies favours the use of these microsatellite loci in popu ...	2009	21564630
golf courses and wetland fauna.	golf courses are often considered to be chemical-intensive ecosystems with negative impacts on fauna. here we provide evidence that golf courses can contribute to the support and conservation of wetland fauna, i.e., amphibians and macroinvertebrates. comparisons of amphibian occurrence, diversity of macroinvetebrates, and occurrence of species of conservation concern were made between permanent freshwater ponds surveyed on golf courses around sweden's capital city, stockholm, and off-course pond ...	2009	19769096
divergence in the face of gene flow: the case of two newts (amphibia: salamandridae).	understanding the process of divergence requires the quantitative characterization of patterns of gene flow between diverging taxa. new and powerful coalescent-based methods give insight into these processes in unprecedented details by enabling the reconstruction of the temporal distribution of past gene flow. here, we use sequence variation at eight nuclear markers and mitochondrial dna (mtdna) in multiple populations to study diversity, divergence, and gene flow between two subspecies of a sal ...	2009	19136451
ranavirus-associated mass mortality in wild amphibians, the netherlands, 2010: a first report.	in 2010, a mass die-off of over 1000 wild water frogs (pelophylax spp.) and at least 10 common newts (lissotriton vulgaris) occurred in a pond in the netherlands. haemorrhagic disease with hepatomegaly and splenomegaly was evident. microscopically, multiple organs presented cells with multifocal intracytoplasmic inclusion bodies, in which ranavirus-like particles were demonstrated ultrastructurally. all specimens examined tested positive for ranavirus by pcr. the sequence obtained showed a 100% ...	2011	21955440
widespread introgression does not leak into allotopy in a broad sympatric zone.	species that overlap over a large part of their range and habitat requirements are challenging for the study of speciation and hybridization. in this respect, the study of broadscale introgressive hybridization has raised recent interest. here we studied hybridization between two closely related amphibians lissotriton helveticus and lissotriton vulgaris that reproduce over a wide sympatric zone. we used mitochondrial and microsatellite markers on 1272 individuals in 37 sites over europe to detec ...	2011	21081968
interspecific hybridization increases mhc class ii diversity in two sister species of newts.	our understanding of the evolutionary mechanisms generating variation within the highly polymorphic major histocompatibility complex (mhc) genes remains incomplete. assessing mhc variation across multiple populations, of recent and ancient divergence, may facilitate understanding of geographical and temporal aspects of variation. here, we applied 454 sequencing to perform a large-scale, comprehensive analysis of mhc class ii in the closely related, hybridizing newts, lissotriton vulgaris (lv) an ...	2012	22066802
asymmetric female preferences for courtship pheromones in two closely-related newt species, the smooth newt (lissotriton vulgaris) and the carpathian newt (l. montandoni) (salamandridae).	the smooth (lissotriton vulgaris) and carpathian (l. montandoni) newts are sister species. these are separated by a moderate genetic distance, but exhibit striking morphological differences, especially in male epigamic traits. in the areas where they co-occur, they readily mate with each other and produce viable hybrids. however, a high level of pre-zygotic isolation with an unknown behavioral basis has been reported. the complex courtship of newts consists of at least three types of modality: c ...	2012	22639810
male attractiveness is influenced by uv wavelengths in a newt species but not in its close relative.	functional communication in the uv range has been reported in invertebrates and all major groups of vertebrates but amphibians. although perception in this wavelength range has been shown in a few species, uv signalling has not been demonstrated in this group. one reason may be that in lentic freshwater habitats, litter decomposition generates dissolved organic carbon that absorbs uv radiation and thus hinders its use for visual signalling. we tested the effect of male uv characteristics on fema ...	2012	22272342
new(t)s and views from hybridizing mhc genes: introgression rather than trans-species polymorphism may shape allelic repertoires.	one of the key features of major histocompatibility complex (mhc) genes is the frequent occurrence of trans-species polymorphism, that is 'the passage of allelic lineages from ancestral to descendant species' (klein et al. 2007). selectively maintained ancestral polymorphism may, however, be hard to distinguish from introgression of mhc alleles between hybridizing species (fig. 1). in this issue of molecular ecology, nadachowska-brzyska et al. (2012) present data that suggest that the latter can ...	2012	22268859
the spinal cord supports of vertebrae in the crown-group salamanders (caudata, urodela).	the development of spinal cord supports (bony thickenings which extend into the vertebral canal of vertebrae) in primitive (salamandrella keyserlingii) and derived (lissotriton vulgaris) salamanders were described. the spinal cord supports develop as the protuberances of periostal bone of the neural arches in the anteroproximal part of the septal collagenous fibers which connect a transverse myoseptum with the notochord and spinal cord, in the septal bundle inside the vertebral canal. spinal cor ...	2012	22707352
hematological- and neurological-expressed sequence 1 gene products in progenitor cells during newt retinal development.	urodele amphibians such as japanese common newts have a remarkable ability to regenerate their injured neural retina, even as adults. we found that hematological- and neurological-expressed sequence 1 (hn1) gene was induced in depigmented retinal pigment epithelial (rpe) cells, and its expression was maintained at later stages of newt retinal regeneration. in this study, we investigated the distribution of the hn1 protein, the product of the hn1 gene, in the developing retinas. our immunohistoch ...	2012	22719773
metamorphosis inhibition: an alternative rearing protocol for the newt, cynops pyrrhogaster.	the newt is an indispensable model animal, of particular utility for regeneration studies. recently, a high-throughput transgenic protocol was established for the japanese common newt, cynops pyrrhogaster. for studies of regeneration, metamorphosed animals may be favorable; however, for this species, there is no efficient protocol for maintaining juveniles after metamorphosis in the laboratory. in these animals, survival drops drastically after metamorphosis as their foraging behaviour changes t ...	2012	22559962
no evidence for nuclear introgression despite complete mtdna replacement in the carpathian newt (lissotriton montandoni).	patterns of interspecific introgression may vary geographically, and the distribution of introgressed variants can yield insight into the historical dynamics of genetic interactions between hybridizing species. urodele amphibians, often characterized by limited mobility, deep intraspecific genetic structuring and vulnerability to climatic changes, constitute suitable models for such historical inferences. here, we combine an extensive survey of the mitochondrial (mtdna) and nuclear (15 microsate ...	2013	23379646
molecular genetic system for regenerative studies using newts.	urodele newts have the remarkable capability of organ regeneration, and have been used as a unique experimental model for more than a century. however, the mechanisms underlying regulation of the regeneration are not well understood, and gene functions in particular remain largely unknown. to elucidate gene function in regeneration, molecular genetic analyses are very powerful. in particular, it is important to establish transgenic or knockout (mutant) lines, and systematically cross these lines ...	2013	23305125
[furosemide-sensitive transport of chloride through the apical membrane of cells of the proximal tubule of the triton kidney].	the effect of furosemide on transtubular electrical potential (vt) as well as on the potentials of apical (va) and basolateral (vb) membranes of the triturus vulgaris proximal nephron cells was studied in alteration of the electrochemical potential gradient na+ and cl- through the apical membrane. furosemide did not change the va and vb provided the concentration of nacl in the lumen of proximal canal was the same as in the blood plasma. when the nacl concentration diminished in the lumen furose ...	2013	4065369
the effects of two retinoids on limb regeneration in pleurodeles waltl and triturus vulgaris.	the effects of two vitamin a analogues, retinol palmitate and retinoic acid, on pattern formation during limb regeneration in larvae of two european newts, p. waltl and t. vulgaris are described. the response of the regenerating limb to retinoid treatment differed according to the larval stage of development for p. waltl. young larval limbs, which were anterior limb buds at the time of amputation, duplicated transversely while limbs of older larvae duplicated proximodistally. proximodistal dupli ...	2013	3723061
cloacal anatomy of the male carpathian newt, lissotriton montandoni (amphibia, salamandridae), in the breeding season.	this study presents the first light microscopy-based description of the cloacal anatomy of male carpathian newts (lissotriton montandoni). this european newt species hybridizes with its sister species, the smooth newt (l. vulgaris), despite a high level of prezygotic isolation. the goal of the study was to ascertain possible anatomical differences in cloacal anatomy, especially pheromone and spermatophore producing glands, which might potentially affect reproductive isolation between l. montando ...	2013	24004081
similar local and landscape processes affect both a common and a rare newt species.	although rare species are often the focus of conservation measures, more common species may experience similar decline and suffer from the same threatening processes. we tested this hypothesis by examining, through an information-theoretic approach, the importance of ecological processes at multiple scales in the great crested newt triturus cristatus, regionally endangered and protected in europe, and the more common smooth newt, lissotriton vulgaris. both species were similarly affected by the ...	2013	23658765
[effect of catecholamines on electrolyte and water transport in the nephron of lower vertebrates].	micropuncture technique and electron microprobe analysis have been used to investigate the role of noradrenalin in ion and water balance in the renal tubules of the lamprey lampetra fluviatilis and newt triturus vulgaris. noradrenalin decreased na, k, and ca concentrations in the proximal lumen of the lamprey increasing the value of (tf/p)in from 1.1 +/- 0.1 to 1.3 +/- 0.1 (p less than 0.001). regitin blocked these effects. noradrenalin perfusion of the peritubular capillaries in newt kidney inc ...	2013	3618016
single nucleotide polymorphisms reveal genetic structuring of the carpathian newt and provide evidence of interspecific gene flow in the nuclear genome.	genetic variation within species is commonly structured in a hierarchical manner which may result from superimposition of processes acting at different spatial and temporal scales. in organisms of limited dispersal ability, signatures of past subdivision are detectable for a long time. studies of contemporary genetic structure in such taxa inform about the history of isolation, range changes and local admixture resulting from geographically restricted hybridization with related species. here we ...	2014	24820116
metacercariae of clinostomum complanatum (trematoda: digenea) in european newts triturus carnifex and lissotriton vulgaris (caudata: salamandridae).	adults of clinostomum spp. are digenetic trematodes found in fish-eating birds, reptiles and occasionally mammals, including humans. freshwater snails serve as first intermediate hosts and many fish species and amphibians as second intermediate hosts. to date, amphibian hosts of clinostomum metacercariae include members of urodele and anuran families in north america, but no data are available on infections of european amphibians, including newts. in this study, we characterize infections of cli ...	2014	23506789
determination of antimicrobial and heavy metal resistance profiles of some bacteria isolated from aquatic amphibian and reptile species.	the aim of the present study was to determine the level of antibiotic resistance patterns and distribution of heavy metal resistance of bacterial isolates from aquatic animals (lissotriton vulgaris, pelophylax ridibundus, emys orbicularis, mauremys rivulata, and natrix natrix) in turkey (kavak delta). a total of 153 bacteria have been successfully isolated from cloaca and oral samples of the aquatic amphibians and reptilians which were found, namely, aeromonas sp. (n = 29), plesiomonas sp. (n = ...	2014	23959346
[photoreceptors and visual pigments in three species of newts].	photoreceptor complement and retinal visual pigments in three newt (caudata, salamandridae, pleurodelinae) species (pleurodeles waltl, lissotriton (triturus) vulgaris and cynops orientalis) were studied by light mucroscopy and microspectrophotometry. retinas of all three species contain "red" (rhodopsin/porphyropsin) rods, large and small single cones, and double cones. large single cones and both components of double cones contain red-sensitive (presumably lws) visual pigment whose absorbance s ...	2014	24459859
can newts cope with the heat? disparate thermoregulatory strategies of two sympatric species in water.	many ectotherms effectively reduce their exposure to low or high environmental temperatures using behavioral thermoregulation. in terrestrial ectotherms, thermoregulatory strategies range from accurate thermoregulation to thermoconformity according to the costs and limits of thermoregulation, while in aquatic taxa the quantification of behavioral thermoregulation have received limited attention. we examined thermoregulation in two sympatric newt species, ichthyosaura alpestris and lissotriton vu ...	2015	25993482
context-dependent plastic response during egg-laying in a widespread newt species.	previous research on predator-induced phenotypic plasticity mostly focused on responses in morphology, developmental time and/or behaviour during early life stages, but the potential significance of anticipatory parental responses has been investigated less often. in this study i examined behavioural and maternal responses of gravid female smooth newts, lissotriton vulgaris, in the presence of chemical cues originating from invertebrate predators, acilius sulcatus water beetles and aeshna cyanea ...	2015	26291328
i environmental dna sampling is more sensitive than a traditional survey technique for detecting an aquatic invader.	effective management of alien species requires detecting populations in the early stages of invasion. environmental dna (edna) sampling can detect aquatic species at relatively low densities, but few studies have directly compared detection probabilities of edna sampling with those of traditional sampling methods. we compare the ability of a traditional sampling technique (bottle trapping) and edna to detect a recently established invader, the smooth newt lissotriton vulgaris vulgaris, at seven ...	2015	26591459
females have larger ratio of second-to-fourth digits than males in four species of salamandridae, caudata.	digit ratio (2d:4d) denotes the relative length of the second and fourth digits. it is considered to be a suitable biomarker of the in utero balance of fetal sex hormones, which affect early development of individuaĺs behavioral and morphological characteristics. in recent decades, digit ratio attracted a great attention in biology and psychology. however, for unmasking the biological basis of the phenomenon, extensive studies on non-human animals are necessary. despite it was hypothesized that ...	2015	25704339
a highly divergent picornavirus in an amphibian, the smooth newt (lissotriton vulgaris).	genetically highly divergent picornavirus (newt/2013/hun, kp770140) was detected using viral metagenomics in faecal samples of free-living smooth newts (lissotriton vulgaris). newt picornavirus was identified by reverse transcription-polymerase chain reaction (rt-pcr) in six (25 %) of the 24 samples originating from individuals caught in two out of the six investigated natural ponds in hungary. the first picornavirus in amphibians expands the host range of members of the picornaviridae, and open ...	2015	26018961
long-term observation of amphibian populations inhabiting urban and forested areas in yekaterinburg, russia.	this article presents data derived from a 36 year-long uninterrupted observational study of amphibian populations living in the city and vicinity of yekaterinburg, russia. this area is inhabited by six amphibian species. based on a degree of anthropogenic transformation, the urban territory is divided into five highly mosaic zones characterized by vegetation, temperature, and a distinctive water pollution profile. population data is presented year-by-year for the number of animals, sex ratio, an ...	2015	25984350
no observable effect of a glyphosate-based herbicide on two top predators of temporal water bodies.	it has been implied that the application of pesticides is involved in the world-wide decline of biodiversity, but little is known about the influence of these chemicals on key predators of temporary wetlands. the direct impacts were examined of a frequently applied glyphosate-based herbicide on larval aeshna cyanea (müller, 1764; odonata, insecta) and adult male lissotriton vulgaris (linnaeus, 1758; caudata, amphibia), 2 top predators of central european ephemeral ponds. the effects of herbicide ...	2015	25378294
the distribution and taxonomy of lissotriton newts in turkey (amphibia, salamandridae).	two and perhaps three taxa of lissotriton newt occur in turkey. their species status is controversial. the distribution of these taxa and the taxonomic status of each are reviewed and discussed. a database of 128 turkish lissotriton localities was compiled and species distribution models were constructed. we reiterate that the presence of lissotriton (vulgaris) lantzi in turkey is disputed and needs confirmation. the range of lissotriton (vulgaris) kosswigi is restricted to north-western anatoli ...	2015	25829839
[radioautographic study of nonhistone protein synthesis in the process of lens and retina regeneration in adult tritons].	the incorporation of 3h-tryptophane (3h-t) in the cell nuclei of pigment epithelium, retinal rudiment, iris and vitreous body has been studied during lens and retina regeneration in the adult smooth newts. a gradual increase (7 to 13-fold as compared with the control) of the intensity of labelling was found in the cell nuclei of pigment epithelium of the central part of fundus oculi. the cells of this zone are a source of regeneration. in the peripheral zones which do not participate in retina r ...	2015	6621999
variation in winter metabolic reduction between sympatric amphibians.	distribution and abundance of temperate ectotherms is determined, in part, by the depletion of their limited caloric reserves during wintering. the magnitude of winter energy drain depends on the species-specific capacity to seasonally modify the minimal maintenance costs. we examined seasonal variation of minimum oxygen consumption between two newt species, ichthyosaura alpestris and lissotriton vulgaris. oxygen consumption was measured in both species during their active season (daily temperat ...	2016	27418441
uv wavelengths experienced during development affect larval newt visual sensitivity and predation efficiency.	we experimentally investigated the influence of developmental plasticity of ultraviolet (uv) visual sensitivity on predation efficiency of the larval smooth newt, lissotriton vulgaris. we quantified expression of sws1 opsin gene (uv-sensitive protein of photoreceptor cells) in the retinas of individuals who had developed in the presence (uv+) or absence (uv-) of uv light (developmental treatments), and tested their predation efficiency under uv+ and uv- light (testing treatments). we found that ...	2016	26843556
comprehensive dna barcoding of the herpetofauna of germany.	we present the first comprehensive dna barcoding study of german reptiles and amphibians representing likewise the first on the european herpetofauna. a total of 248 barcodes for all native species and subspecies in the country and a few additional taxa were obtained in the framework of the projects 'barcoding fauna bavarica' (bfb) and 'german barcode of life' (gbol). in contrast to many invertebrate groups, the success rate of the identification of mitochondrial lineages representing species vi ...	2016	25892157
musculoskeletal architecture of the prey capture apparatus in salamandrid newts with multiphasic lifestyle: does anatomy change during the seasonal habitat switches?	some newt species change seasonally between an aquatic and a terrestrial life as adults, and are therefore repeatedly faced with different physical circumstances that affect a wide range of functions of the organism. for example, it has been observed that seasonally habitat-changing newts display notable changes in skin texture and tail fin anatomy, allowing one to distinguish an aquatic and a terrestrial morphotype. one of the main functional challenges is the switch between efficient aquatic a ...	2016	26892189
expanding distribution of lethal amphibian fungus batrachochytrium salamandrivorans in europe.	emerging fungal diseases can drive amphibian species to local extinction. during 2010-2016, we examined 1,921 urodeles in 3 european countries. presence of the chytrid fungus batrachochytrium salamandrivorans at new locations and in urodeles of different species expands the known geographic and host range of the fungus and underpins its imminent threat to biodiversity.	2016	27070102
divergence history of the carpathian and smooth newts modelled in space and time.	information about demographic history is essential for the understanding of the processes of divergence and speciation. patterns of genetic variation within and between closely related species provide insights into the history of their interactions. here, we investigated historical demography and genetic exchange between the carpathian (lissotriton montandoni, lm) and smooth (l. vulgaris, lv) newts. we combine an extensive geographical sampling and multilocus nuclear sequence data with the appro ...	2016	27288862
phenotypic flexibility of gape anatomy fine-tunes the aquatic prey-capture system of newts.	a unique example of phenotypic flexibility of the oral apparatus is present in newts (salamandridae) that seasonally change between an aquatic and a terrestrial habitat. newts grow flaps of skin between their upper and lower jaws, the labial lobes, to partly close the corners of the mouth when they adopt an aquatic lifestyle during their breeding season. using hydrodynamic simulations based on μct-scans and cranial kinematics during prey-capture in the smooth newt (lissotriton vulgaris), we show ...	2016	27383663
costly neighbours: heterospecific competitive interactions increase metabolic rates in dominant species.	the energy costs of self-maintenance (standard metabolic rate, smr) vary substantially among individuals within a population. despite the importance of smr for understanding life history strategies, ecological sources of smr variation remain only partially understood. stress-mediated increases in smr are common in subordinate individuals within a population, while the direction and magnitude of the smr shift induced by interspecific competitive interactions is largely unknown. using laboratory e ...	2017	28701786
influence of temperature on female, embryonic and hatchling traits in syntopic newts, ichthyosaura alpestris and lissotriton vulgaris.	amphibian populations have been declining globally for the last several decades, and climate change is often regarded as one of the most important factors driving these declines. amphibians are particularly sensitive to climatic changes due to their physiological, ecological and behavioral characteristics. here we performed a laboratory experiment to investigate how temperature affects ovipositing females, eggs and hatchlings in two syntopic populations of alpine newts, ichthyosaura alpestris, a ...	2017	28010812
genetic characterization of a second novel picornavirus from an amphibian host, smooth newt (lissotriton vulgaris).	in this study, a novel picornavirus was identified in faecal samples from smooth newts (lissotriton vulgaris). the complete genome of picornavirus strain newt/ii-5-pilis/2014/hun (kx463670) is 7755 nt long with type-iv ires and has 39.6% aa sequence identity in the protein p1 to the corresponding protein of bat picornavirus (kj641686, unassigned) and 42.7% and 53.5% aa sequence identity in the 2c and 3cd protein, respectively, to oscivirus (gu182410, genus oscivirus). interestingly, the l-protei ...	2017	28005212
flexibility is everything: prey capture throughout the seasonal habitat switches in the smooth newt lissotriton vulgaris.	transitions between aquatic and terrestrial habitats are significant steps in vertebrate evolution. due to the different biophysical demands on the whole organism in water and air, such transitions require major changes of many physiological functions, including feeding. accordingly, the capability to modulate the pre-programmed chain of prey-capture movements might be essential to maintain performance in a new environment. newts are of special interest in this regard as they show a multiphasic ...	2017	26097413
linkage map of lissotriton newts provides insight into the genetic basis of reproductive isolation.	linkage maps are widely used to investigate structure, function, and evolution of genomes. in speciation research, maps facilitate the study of the genetic architecture of reproductive isolation by allowing identification of genomic regions underlying reduced fitness of hybrids. here we present a linkage map for european newts of the lissotriton vulgaris species complex, constructed using two families of f2 l. montandoni × l. vulgaris hybrids. the map consists of 1146 protein-coding genes on 12 ...	2017	28500054
facilitating permeability of landscapes impacted by roads for protected amphibians: patterns of movement for the great crested newt.	amphibian populations are highly vulnerable to road mortality and habitat fragmentation caused by road networks. wildlife road tunnels are considered the most promising road mitigation measure for amphibians yet generally remain inadequately monitored, resulting in mixed success rates in the short-term and uncertain conservation benefits in the long-term. we monitored a complex multi-tunnel and fence system over five years and investigated the impact of the scheme on movement patterns of two new ...	2017	28265490
amphibian recovery after a decrease in acidic precipitation.	we here report the first sign of amphibian recovery after a strong decline due to acidic precipitation over many decades and peaking around 1980-90. in 2010, the ph level of ponds and small lakes in two heavily acidified areas in southwestern scandinavia (aust-agder and østfold in norway) had risen significantly at an (arithmetic) average of 0.14 since 1988-89. parallel with the general rise in ph, amphibians (rana temporaria, r. arvalis, bufo bufo, lissotriton vulgaris, and triturus cristatus) ...	2017	29164539
models of amphibian myogenesis - the case of bombina variegata.	several different models of myogenesis describing early stages of amphibian paraxial myotomal myogenesis are known. myoblasts of xenopus laevis and hymenochirus boettgeri change their position from perpendicular to parallel, in relation to axial organs, and differentiate into mononucleate myotubes. in bombina variegate the myotomal myoblasts change their shape from round to elongate and then differentiate into mononuclear, morphologically mature myotubes. in pelobates fuscus and triturus vulgari ...	2017	28287243
amphibian skin microbiota exhibits temporal variation in community structure but stability of predicted bd-inhibitory function.	host-associated microbiomes are increasingly recognized to contribute to host disease resistance; the temporal dynamics of their community structure and function, however, are poorly understood. we investigated the cutaneous bacterial communities of three newt species, ichthyosaura alpestris, lissotriton vulgaris and triturus cristatus, at approximately weekly intervals for 3 months using 16s ribosomal rna amplicon sequencing. we hypothesized cutaneous microbiota would vary across time, and that ...	2017	28387770
effects of a glyphosate-based herbicide and predation threat on the behaviour of agile frog tadpoles.	the widespread application of pesticides emphasises the importance of understanding the impacts of these chemicals on natural communities. the most commonly applied broad-spectrum herbicides in the world are glyphosate-based herbicides, which have been suggested to induce significant behavioural changes in non-target organisms even at low environmental concentrations. to scrutinize the behavioural effects of herbicide-exposure we exposed agile frog (rana dalmatina) tadpoles in an outdoor mesocos ...	2017	28242374
unraveling climate influences on the distribution of the parapatric newts lissotriton vulgaris meridionalis and l. italicus.	climate is often considered as a key ecological factor limiting the capability of expansion of most species and the extent of suitable habitats. in this contribution, we implement species distribution models (sdms) to study two parapatric amphibians, lissotriton vulgaris meridionalis and l. italicus, investigating if and how climate has influenced their present and past (last glacial maximum and holocene) distributions. a database of 901 gps presence records was generated for the two newts. sdms ...	2017	29255477
isolation and gene flow in a speciation continuum in newts.	because reproductive isolation often evolves gradually, differentiating lineages may retain the potential for genetic exchange for prolonged periods, providing an opportunity to quantify and to understand the fundamental role of gene flow during speciation. here we delimit evolutionary lineages, reconstruct the phylogeny and infer gene flow in newts of the lissotriton vulgaris species complex based on 74 nuclear markers sampled from 127 localities. we demonstrate that distinct lineages along the ...	2017	28797693
bony skull of the smooth newt triturus vulgaris (amphibia, urodela: salamandridae) and the role of thyroid hormones in its ossification.		2017	12705137
characteristics of development and ossification of skeletal elements in the limbs of the smooth newt triturus vulgaris (caudata).		2017	12577639
an improved microsatellite panel to assess genetic variability of the italian smooth newt (lissotriton vulgaris meridionalis).	habitat loss and fragmentation are threatening amphibians by increasing population isolation. however, artificial waterbodies created for livestockmay contrast this phenomenon by providing surrogate habitats for amphibians. here, we performed a genetic study on an amphibian species, lissotriton vulgaris meridionalis, in a rural area in central italy where natural wetlands are disappearing and drinking troughs for cattle watering are widespread. specifically, we tested a panel of microsatellite m ...	2018	29932078
egg-laying environment modulates offspring responses to predation risk in an amphibian.	predator-induced plasticity has been in the focus of evolutionary ecological research in the last decades, but the consequences of temporal variation in the presence of cues predicting offspring environment have remained controversial. this is partly due to the fact that the role of early environmental effects has scarcely been scrutinized in this context while also controlling for potential maternal effects. in this study, we investigated how past environmental conditions, that is different com ...	2018	29485239
no trade-offs in interspecific interference ability and predation susceptibility in newt larvae.	coexistence of species with similar requirements is allowed, among others, through trade-offs between competitive ability and other ecological traits. although interspecific competition is based on two mechanisms, exploitation of resources and physical interference, trade-off studies largely consider only species' ability to exploit resources. using a mesocosm experiment, we examined the trade-off between interference competition ability and susceptibility to predation in larvae of two newt spec ...	2018	30271569
differential introgression across newt hybrid zones: evidence from replicated transects.	genomic heterogeneity of divergence between hybridizing species may reflect heterogeneity of introgression, but also processes unrelated to hybridization. heterogeneous introgression and its repeatability can be directly tested in natural hybrid zones by examining multiple transects. here, we studied hybrid zones between the european newts lissotriton montandoni and two lineages of lissotriton vulgaris, with replicate transects within each zone. over 1,000 nuclear genes located on a linkage map ...	2019	31549466
mosquito control based on bacillus thuringiensis israelensis (bti) interrupts artificial wetland food chains.	the biocide bacillus thuringiensis israelensis (bti) has become the most commonly used larvicide to control mosquitoes in seasonal wetlands. although bti is considered non-toxic to most aquatic organisms, the non-biting chironomids show high susceptibilities towards bti. as chironomids are a key element in wetland food webs, major declines in their abundance could lead to indirect effects that may be passed through aquatic and terrestrial food chains. we conducted two mesocosm experiments to add ...	2019	31412513
underwater sound production varies within not between species in sympatric newts.	sound production is a widespread phenomenon among animals. effective sound use for mate or species recognition requires some acoustic differentiation at an individual or species level. several species of caudate amphibians produce underwater sounds, but information about intra- and interspecific variation in their acoustic production is missing. we examined individual, sex, and species variation in underwater sound production in adults of two sympatric newt taxa, ichthyosaura alpestris and lisso ...	2019	30944780
helminth endoparasites of the smooth newt lissotriton vulgaris: linking morphological identification and molecular data.	the helminth endoparasites of many european amphibian species are often known exclusively from morphological descriptions. a molecular library of dna sequence data linked to morphological identifications is still in its infancy. in this paper, we aim to contribute to such a library on the smooth newt lissotriton vulgaris, the intermediate and definitive host of 31 helminth parasites, according to evidence published so far. newts (n = 69) were collected at two study sites in western germany and e ...	2019	29502544
captivity and infection by the fungal pathogen batrachochytrium salamandrivorans perturb the amphibian skin microbiome.	the emerging fungal pathogen, batrachochytrium salamandrivorans (bsal) is responsible for the catastrophic decline of european salamanders and poses a threat to amphibians globally. the amphibian skin microbiome can influence disease outcome for several host-pathogen systems, yet little is known of its role in bsal infection. in addition, many experimental in-vivo amphibian disease studies to date have relied on specimens that have been kept in captivity for long periods without considering the ...	2019	31507541
common midwife toad ranaviruses replicate first in the oral cavity of smooth newts (lissotriton vulgaris) and show distinct strain-associated pathogenicity.	ranavirus is the second most common infectious cause of amphibian mortality. these viruses affect caudates, an order in which information regarding ranavirus pathogenesis is scarce. in the netherlands, two strains (cmtv-nl i and iii) were suspected to possess distinct pathogenicity based on field data. to investigate susceptibility and disease progression in urodeles and determine differences in pathogenicity between strains, 45 adult smooth newts (lissotriton vulgaris) were challenged via bath ...	2019	30872735