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tests for sexual incompatibility between two newt species, triturus vulgaris and triturus montandoni: no-choice mating design. 199728565101
unilateral forelimb amputation affects protein synthesis in ipsilateral and contralateral spinal cord and spinal ganglia neurons of the newt in vivo.the aim of this investigation was to analyze the effect of urodele limb amputation and blastema growth on the protein synthesis in spinal cord and spinal ganglia neurons corresponding to the 3rd and 4th spinal segment of young, postmetamorphic triturus vulgaris, in vivo. protein synthesis was studied on both trunk sides as a function of right forelimb amputation at the distal stylopodium, and blastema development, during a critical period of 22 days post amputation (dpa). protein synthesis was a ...19938168437
cobalt-dependent stimulation of sodium transport in the amphibian skin and nephron.one to ten millimolar cocl2, when applied to the outer surface of the apical membrane of the frog skin (rana temporaria), reversibly increased the potential differences and short-circuit current. these observations suggest that co2+ may control the gating system of the sodium channel. in the kidney of the newt (triturus vulgaris), proximal reabsorption is increased under the influence of 0.5 mm cocl2 injected into the lumen. when cocl2 (0.5 mm) was injected into the lumen of the distal tubule of ...19862873934
asymmetric female preferences for courtship pheromones in two closely-related newt species, the smooth newt (lissotriton vulgaris) and the carpathian newt (l. montandoni) (salamandridae).the smooth (lissotriton vulgaris) and carpathian (l. montandoni) newts are sister species. these are separated by a moderate genetic distance, but exhibit striking morphological differences, especially in male epigamic traits. in the areas where they co-occur, they readily mate with each other and produce viable hybrids. however, a high level of pre-zygotic isolation with an unknown behavioral basis has been reported. the complex courtship of newts consists of at least three types of modality: c ...201222639810
male attractiveness is influenced by uv wavelengths in a newt species but not in its close relative.functional communication in the uv range has been reported in invertebrates and all major groups of vertebrates but amphibians. although perception in this wavelength range has been shown in a few species, uv signalling has not been demonstrated in this group. one reason may be that in lentic freshwater habitats, litter decomposition generates dissolved organic carbon that absorbs uv radiation and thus hinders its use for visual signalling. we tested the effect of male uv characteristics on fema ...201222272342
new(t)s and views from hybridizing mhc genes: introgression rather than trans-species polymorphism may shape allelic repertoires.one of the key features of major histocompatibility complex (mhc) genes is the frequent occurrence of trans-species polymorphism, that is 'the passage of allelic lineages from ancestral to descendant species' (klein et al. 2007). selectively maintained ancestral polymorphism may, however, be hard to distinguish from introgression of mhc alleles between hybridizing species (fig. 1). in this issue of molecular ecology, nadachowska-brzyska et al. (2012) present data that suggest that the latter can ...201222268859
interspecific hybridization increases mhc class ii diversity in two sister species of newts.our understanding of the evolutionary mechanisms generating variation within the highly polymorphic major histocompatibility complex (mhc) genes remains incomplete. assessing mhc variation across multiple populations, of recent and ancient divergence, may facilitate understanding of geographical and temporal aspects of variation. here, we applied 454 sequencing to perform a large-scale, comprehensive analysis of mhc class ii in the closely related, hybridizing newts, lissotriton vulgaris (lv) an ...201222066802
widespread introgression does not leak into allotopy in a broad sympatric zone.species that overlap over a large part of their range and habitat requirements are challenging for the study of speciation and hybridization. in this respect, the study of broadscale introgressive hybridization has raised recent interest. here we studied hybridization between two closely related amphibians lissotriton helveticus and lissotriton vulgaris that reproduce over a wide sympatric zone. we used mitochondrial and microsatellite markers on 1272 individuals in 37 sites over europe to detec ...201121081968
[microscopic anatomy of the triton kidney].the structure of the nephron, length of all its segments and the renal architectonics as a whole have been studied in the newt (triturus vulgaris l.) by means of microdissection and microinjection methods. the microinjection of latex or lissamine green is performed into the blood vessels and nephrons. in one kidney 85-95 nephrons are counted, their number is not the same along the kidney length and increases in the caudal direction. there are nephrons of the ventral, intrarenal and medial popula ...19846712491
single channel ion currents in embryonic cells of the newt (triturus vulgaris) isolated without enzymatic treatment. 19836318520
[radioautographic study of nonhistone protein synthesis in the process of lens and retina regeneration in adult tritons].the incorporation of 3h-tryptophane (3h-t) in the cell nuclei of pigment epithelium, retinal rudiment, iris and vitreous body has been studied during lens and retina regeneration in the adult smooth newts. a gradual increase (7 to 13-fold as compared with the control) of the intensity of labelling was found in the cell nuclei of pigment epithelium of the central part of fundus oculi. the cells of this zone are a source of regeneration. in the peripheral zones which do not participate in retina r ...20156621999
the activity of the nucleus preopticus during limb regeneration in adult newts, triturus vulgaris. 19817262354
new polymorphic microsatellite loci for the greek smooth newt, lissotriton vulgaris graecus, and their utility in the nominotypical subspecies.eight polymorphic microsatellites were isolated from the greek smooth newt (lissotriton vulgaris graecus) using a microsatellite enrichment protocol and selective hybridization with a biotinylated (ac)(11) probe. the loci showed different variation patterns in a single breeding population (32 individuals) with mean number of alleles at 5.0 and mean observed heterozygosity at 0.520. the amplification success also in the nominotypical subspecies favours the use of these microsatellite loci in popu ...200921564630
multiple sex pheromone genes are expressed in the abdominal glands of the smooth newt (lissotriton vulgaris) and montandon's newt (l. montandoni) (salamandridae).the smooth newt (lissotriton "triturus" vulgaris) and montandon's newt (l."t." montandoni) are sister species exhibiting pronounced differences in male secondary sexual traits but nevertheless hybridizing and producing fertile hybrids in nature. since pheromonal communication is an important aspect of the reproductive biology of urodeles, structural differentiation of peptide pheromones and their receptors may contribute to incipient reproductive isolation. the aim of the study was the identific ...200818624569
genetic dissimilarity predicts paternity in the smooth newt (lissotriton vulgaris).under sperm competition, paternity is apportioned by polyandrous females according to the order of matings and the genetic quality of the inseminating males. in order to distinguish between these two effects, we sequentially paired 12 female smooth newts (lissotriton vulgaris) with each of two males and, where possible, repeated the same procedure in reverse order of the identical males after assumed sperm depletion. for a total of 578 offspring, amplified fragment length polymorphisms genetic m ...200717638673
an experimental analysis of bivalent interlocking in spermatocytes of the new triturus vulgaris.interlocked bivalents at 1st meiotic metaphase are relatively uncommon in spermatocytes of the newt triturus vulgaris, but their frequency of occurrence can be significantly increased by subjecting newts to a 24-h heat shock. newt spermatocytes are sensitive to a heart shock at any stage between the end of premeiotic s and mid to late pachytene. the heat shock does not cause evidence desynapsis, nor does it significantly affect chiasma frequency; therefore the interlocked condition induced in sp ...1979479321
effects of ph and aluminium on the growth and feeding behaviour of smooth and palmate newt larvae.: the distribution of smooth and palmate newts (triturus vulgaris and triturus helveticus) may be related to water quality. this study compared the larval growth and feeding behaviour of both species under sublethal levels of aluminium and low ph. the growth of both species was inhibited to a similar degree under acidic conditions with aluminium toxicity dependent upon both low ph and developmental stage. the two species were differentially affected by low ph in terms of feeding behaviour. t. vu ...199524197797
modulation of the effect of arginine-vasopressin on water and ion transport in the newt early distal tubule and frog urinary bladder by v1-antagonists.in the early distal tubule of the newt triturus vulgaris l., 1 nm arginine-vasopressin (avp) increased water reabsorption; the fractional reabsorption of na+ was elevated from 46.2 +/- 6.9% to 67.8 +/- 3.9% (p < 0.001), of cl- from 52.7 +/- 6.7% to 73.1 +/- 3.5% (p < 0.001), of mg2+ from 48.0 +/- 7.7% to 71.7 +/- 6.3% (p < 0.001). when v1-receptors were blocked by 1 nm peptide v1-antagonist [1-(beta-mercapto-beta,beta-cyclopentamethylene propionic acid), 2-(o-methyl) tyr]-[arg8]vasopressin, 1 nm ...19958594534
molecular structure of the rdna intergenic spacer (igs) in triturus: implications for the hypervariability of rdna loci.ribosomal dna (rdna) variation in the species triturus vulgaris meridionalis (amphibia, urodela) is remarkable because of unusually high intraspecific variability in the number and distribution of ribosomal loci in the karyotype; in addition, portions of the intergenic spacer (igs) are clustered at chromosomal loci where they are not associated with ribosomal 18s and 28s rna genes. these clusters are referred to as extraribosomal, and they appear to consist mostly of repetitive bamhi elements. i ...19979297510
influence of longitudinal whole animal clinorotation on lens, tail, and limb regeneration in urodeles.two species of newts (urodela) and two types of clinostats for fast clinorotation (60 rpm) were used to investigate the influence of simulated weightlessness on regeneration and to compare results obtained with data from spaceflight experiments. seven or fourteen days of weightlessness in russian biosatellites caused acceleration of lens and limb regeneration by an increase in cell proliferation, differentiation, and rate of morphogenesis in comparison with ground controls. after a comparable ti ...199611538637
molecular organization of ribosomal rna genes clustered at variable chromosomal sites in triturus vulgaris meridionalis (amphibia, urodela).the ribosomal rna genes of triturus vulgaris meridionalis (amphibia, urodela) show the peculiar feature of being clustered not only at the nucleolar organizer, present in the species at a definite chromosome location, but also at "additional ribosomal sites" which are highly variable in number and chromosomal distribution among individuals. the additional ribosomal sites are most often found at specific chromosome regions, such as telomeres, c-bands and centromeres, in virtually all the chromoso ...19853003361
blood supply to the amphibian mesonephros.the purpose of the present study was to describe geometry of branching, topography and sources of blood vessels supplying the mesonephros of three different species of amphibia and to infere from the results their common and species-specific features. the detailed information on arrangement of portal and arterial blood supply to mesonephros is necessary for better understanding of the conditions under which these kidneys function. for this reason the blood vessels of the mesonephros of adult hyn ...19937949400
ultrastructural observations on blood vessels surrounding normal and regenerating spinal cord in newt.the ultrastructural analysis of capillaries surrounding normal and regenerating caudal spinal cords of two species of newt (triturus vulgaris and t. cristatus) is reported. around normal spinal cords, capillaries were generally continuous. surrounding the regenerating spinal cords the various capillaries of the regenerating blastema appeared discontinuous with small or broad gaps along the capillary wall. this was seen even after two months of tail regeneration when the spinal cord was similar t ...20061303068
a newt ribozyme: a catalytic activity in search of a function.we analyzed the cleavage properties and the transcription regulation of the newt (triturus vulgaris meridionalis) self-cleaving rna. in vitro self-cleavage of model oligoribonucleotides occurs within a double hammerhead structure. in addition, an entire ribozyme molecule, as well as its catalytic domain, "trans-cleaves" in vitro appropriate oligoribonucleotide substrates. signals encoded within the ribozyme dna sequences regulate the ribozyme transcription, which is rna polymerase ii dependent. ...19921542657
changes in the thermal denaturation profiles of dna from different developmental stages of the newt triturus vulgaris.the melting profiles of dna samples from the early gastrula and early neurula of triturus vulgaris are essentially the same, whereas dna from mid to late gastrula possesses higher tm values and shows a deviation from the regular sigmoidal shape at temperatures above tm. the plot on normal probability paper indicates a second dna fraction which melts at higher temperatures and, consequently, it has a higher gc-content than the bulk of dna. these facts confirm our idea that differential dna replic ...1977923733
chromosome location of the ribosomal rna genes in triturus vulgaris meridionalis (amphibia, urodela). ii. intraspecific variability in number and position of the chromosome loci for 18s + 28s ribosomal rna.ribosomal genes have been localized on mitotic and lampbrush chromosomes of 20 specimens of triturus vulgaris meridionalis by in situ hybridization with 3h 18s + 28s rrna. the results may be summarized as follows: 1) each individual shows positive in situ hybridization at the nucleolus organizing region (nor) on chromosome xi; 2) in addition, many specimens exhibit a positive reaction in chromosomal sites other than the nor (additional ribosomal sites); 3) the chromosomal distribution of the add ...1977598264
towards a model of the courtship of the smooth newt triturus vulgaris, with special emphasis on problems of observatility in the simulation of behaviour. 1977194124
single nucleotide polymorphisms reveal genetic structuring of the carpathian newt and provide evidence of interspecific gene flow in the nuclear genome.genetic variation within species is commonly structured in a hierarchical manner which may result from superimposition of processes acting at different spatial and temporal scales. in organisms of limited dispersal ability, signatures of past subdivision are detectable for a long time. studies of contemporary genetic structure in such taxa inform about the history of isolation, range changes and local admixture resulting from geographically restricted hybridization with related species. here we ...201424820116
cloacal anatomy of the male carpathian newt, lissotriton montandoni (amphibia, salamandridae), in the breeding season.this study presents the first light microscopy-based description of the cloacal anatomy of male carpathian newts (lissotriton montandoni). this european newt species hybridizes with its sister species, the smooth newt (l. vulgaris), despite a high level of prezygotic isolation. the goal of the study was to ascertain possible anatomical differences in cloacal anatomy, especially pheromone and spermatophore producing glands, which might potentially affect reproductive isolation between l. montando ...201324004081
differentiation of liquor contacting neurons in the regenerating spinal cord of the newt triturus.this paper reports a detailed electron microscopic study of the ventricular cells in the growing spinal cord during the first two months of tail regeneration in triturus vulgaris and t. cristatus. in the proliferating ependyma some cells undergo a neural differentiation in order to produce "cerebro-spinal-fluid-contacting-neurons (csfcns). these round or pear-like cells store dense core vesicles, 80-100 nm in diameter, and show less electron-density than ependymal elements. the stereociliar appa ...19892636002
heterochromatic dna in triturus (amphibia, urodela) ii. a centromeric satellite dna.the mspi family of highly repeated sequences is a centromeric satellite dna representing about 1% of the genome of the italian smooth newt, triturus vulgaris meridionalis. we have studied the structure, genomic organization, chromosomal localization and conservation across species of this family. mspi sequences are around 197 bp long, as shown by sequencing of three cloned units. the family is organized in large clusters of tandemly arrayed units, present at almost all the centromeres of t.v. me ...19883219917
are there insertions in the ribosomal dna of vertebrates?we have analysed the eco ri restriction pattern of rdna of the newt triturus vulgaris and of some other amphibian species by southern blotting and hybridization with nick-translated xenopus rdna prepared from the recombinant plasmids pxlr11 and pxlr12 (21). after hybridization with r11, the 28s coding fragments become visible in two bands, a prominent one of 5.3 kb and a weak band of 5.9 kb representing about 8% of the 28s genes. the evidence obtained so far by additional digestions with bam hi ...19852991856
the structure of the alimentary canal and the dimensions of the mucosa in the smooth newt (triturus vulgaris l.). 19761016898
[ultrastructure of the kidney of the newt].in the newt [triturus vulgaris (j.)] kidney a zone of single nephrons is distinguished, where there are ten nephrons, connected with the paramesonephral (müller's) canal, and another zone of the renal mass; in the latter the main part of nephrons is situated, which are connected with the ureter by means of collecting tubules. in these zones of the kidney ultrastructure of epitheliocytes in the proximal and distal parts is different. the renal corpuscles of the epithelial cell in the cervical and ...19854051783
[relative characteristics of the duration of embryonic development in triturus vulgaris].the data of valouch et al. (1971) on tau 0 values in the smooth newt at different temperatures are presented as a curve. using this curve, the absolute (in hours and days) duration of successive developmental stages provided by glaesner (1925) is transformed in a relative one, expressed in the number of tau 0 (tau n/tau 0).20066739036
genic diversity and population genetic structure of triturus vulgaris (urodela, salamandridae). 198428555914
ultrastructural studies of oogenesis in some european amphibians. ii. triturus vulgaris. 19734766454
contribution to study of the arterial supply of the gastrointestinal tract of the newt (triturus vulgaris). 19734798659
the control of alternating coordination of limb pairs in the newt (triturus vulgaris). 19734789785
[annual cyclic studies of catecholamines in the hypothalamus of triturus vulgaris (l.)]. 19734207950
qualitative changes in dna indicating differential dna replication during early embryogenesis of the newt triturus vulgaris.during gastrulation of the newt triturus vulgaris considerable changes in the melting behaviour and in the cscl density gradient pattern of dna occur. the melting curves of dnas from mid to late gastrulae (stages 11b-12c) deviate from the regular shape. whereas the tm values are identical in the stages 11a (early gastrula) and 15 (early neurula), and correspond to the standard dna (stage 36 = tailbud), a significant rise of tm (0.8-1.2 degrees c) has been recorded in the stages 12a/b (yolk plug) ...19807430936
protein synthesis in the mesodermal tissue of forelimb regenerates of triturus vulgaris: an autoradiographic study. 19883412340
[effect of catecholamines on electrolyte and water transport in the nephron of lower vertebrates].micropuncture technique and electron microprobe analysis have been used to investigate the role of noradrenalin in ion and water balance in the renal tubules of the lamprey lampetra fluviatilis and newt triturus vulgaris. noradrenalin decreased na, k, and ca concentrations in the proximal lumen of the lamprey increasing the value of (tf/p)in from 1.1 +/- 0.1 to 1.3 +/- 0.1 (p less than 0.001). regitin blocked these effects. noradrenalin perfusion of the peritubular capillaries in newt kidney inc ...20133618016
the maps of the lampbrush chromosomes of triturus (amphibia urodela). iv. triturus vulgaris meridionalis. 19705489360
spontaneous aberrations in lampbrush chromosome xi from a specimen of triturus vulgaris meridionalis (amphibia, urodela). 19705474809
[cytophotometric study of the non-homogeneity of the cell nucleus during the process of differentiation in the mesoderm of triturus vulgaris]. 19705472238
[cytochemical demonstration of phase- and region-specific changes in dna content during the embryogenesis of triturus vulgaris]. 19695365872
a radioautographic study of the time course of male meiosis in the newt triturus vulgaris. 19685707855
nuclear transplantations in triturus vulgaris l. 196414135332
radioautographic studies or regeneration in the common newt. iii. regeneration and repair of the intestine. 196214480925
radioautographic studies of regeneration in the common newt. 1. physiological regeneration. 196013730117
[helminths in triturus vulgaris l. in the lublin region]. 195813604232
[heteroplastic regeneration of chimeric extremities in triturus vulgaris and triturus cristatus]. 195514380257
[first research on larval parabiosis of f1 hybrids (triturus cristatus male times triturus vulgaris female) with the paternal species]. 200614801217
activity of pars tuberalis of the hypophysis during limb regeneration in adult triturus vulgaris. 19807450089
tissue-specific changes in nuclear rna content during early development of triturus vulgaris.during early development of triturus vulgaris, as a measure for nuclear activity in neuroectoderm, mesoderm and endoderm, nuclear rna content was determined by cytochemical methods. in the first stages of gastrulation, that is to say during the early phase of neural induction, the rna content of the inducing system is considerably higher than in the reacting system. then, with a phase-shift of about 10 h, the rna content of the neuroectoderm increases quickly also. in the following stages the nu ...1979511597
scanning and transmission electron microscopy of intraventricular dendrite terminals of hypothalamic cerebrospinal fluid contacting neurons in triturus vulgaris.a scanning (sem) and transmission electron microscopic (tem) study of the ventricular wall of the hypothalamus of triturus vulgaris was performed with special regard to the intraventricular dendrite terminals of the cerebrospinal fluid (csf) contacting neurons of the preoptic area (magnocellular and parvocellular preoptic nuclei), the infundibular lobe (anterior periventricular nucleus, infundibular nucleus), and the paraventricular organ. in the preoptic area and infundibular lobe, the terminal ...1979524982
the influence of furosemide and co2+ on electrolyte and water transport in newt distal tubule and frog skin.the technique of stationary microperfusion of the early distal tubule used in experiments on male newts (triturus vulgaris) revealed the intratubular effect of cocl2 and furosemide on the ion transport. cocl2 in concentration 5 x 10(-4) m increased na and cl reabsorption, reduced ca, mg and k reabsorption, and increased (tfexp/tfo)in from 1.14 +/- 0.04 to 1.68 +/- 0.27 (p less than 0.001). cocl2 in concentration 5 x 10(-5) m enhanced na and cl reabsorption, without affecting transport of other i ...19862423960
[3h-thymidine incorporation into the macrophages in the process of eye regeneration in adult tritons].changes in the number of labelled macrophages in the regenerating eye cavity of the adult common newts were studied within 2 to 12 days following the injection of 3h-thymidine and removal of retina and lens from the eyes. a small number of labelled macrophages was found in the eye cavity (0,2--5.9%) at all regeneration stages under study upon the pulse 3h-thymidine incorporation. their number rapidly increased and attained by the end of observation (12 days) 73%. these results suggest that mainl ...1979481852
length polymorphism in rdna indicates somatic alterations in the genome of triturus vulgaris.we have analyzed the ecori restriction pattern of ribosomal genes (rdna) isolated from several organs of single individuals of the newt triturus vulgaris by southern blotting and hybridization with corresponding xenopus probes (r11 and r12). using length polymorphism of rdna spacers as a molecular marker, it became evident that in individual newts the pattern of ribosomal genes is not always constant but varies between different tissues of the same animal suggesting the occurrence of genome alte ...19863007245
heterochromatic dna in triturus (amphibia, urodela). i. a satellite dna component of the pericentric c-bands.we have studied the structure, genome organization, chromosomal location, conservation across species and transcription on lampbrush chromosomes, of an at-rich satellite dna component of the newt, triturus vulgaris meridionalis. the satellite (sat g), originally isolated by gradient centrifugation, represents about 2% of the vulgaris genome and comprises a highly repetitive sequence family (hindiii family), whose monomers have been cloned. the repeat units are about 330 bp long, as measured on g ...19863013516
chromosome location of the ribosomal genes in triturus vulgaris meridionalis (amphibia urodela). iii. inheritance of the chromosomal sites for 18s + 28s ribosomal rna.in triturus vulgaris meridionalis, the 18s + 28s rdna sequences have been shown to be located in a number of additional chromosomal sites besides the nucleolus organizing region. the additional ribosomal sites have been found to vary as to their number and chromosomal location in different individuals of the species.--the data presented in this study concern the chromosomal distribution of the ribosomal sequences as analyzed by in situ hybridization technique in two individuals as well as in the ...1978630997
[late labelling of pigment epithelial and iridic cells in smooth newts].the late labelling of the actively proliferating cells of iris and pigment epithelium in the regenerating eye was found in the adult smooth newts within 2 to 12 days following the intraperitoneal injection of 3h-thymidine. this late labelling is due to the long-term presence of the labelled precursors of dna synthesis in the organism and to their reutilization. the index of labelled nuclei upon reutilization in the pigment epithelium is twice and in the iris 3 to 4 times higher than in the exper ...1978724207
rejection of skin allografts in two geographically distant populations of triturus vulgaris (l.) and t. cristatus (laurenti). 1978361175
a comparative cytochemical study of the yolk platelets in the growing oocytes of triturus cristatus and rana esculenta.recent cytochemical techniques for the detection of proteins, neutral and acid mucopolysaccharides, lipids and phospholipids in the yolk platelets of the growing oocytes of rana esculenta and triturus vulgaris are employed. cytochemical analysis showed that the yolk in the form of granules or platelets, in the constituting itself the most prominent cytoplasmic inclusion of amphibian eggs, contains a protein moiety consisting of individual typical amino acids lacking tryptophan in triturus, a car ...197773338
the surface charge of mesodermal and neural cells of triturus embryos.the electrical surface charge of disaggregated mesodermal and neural cells of the neurula stage of triturus vulgaris embryos is estimated by using the apparatus described in figure 1. the results showed a significant difference between the net charges of these two cell types: the neural cells had a clearly negative charge, whereas the mesodermal cells seemed to be more or less neutral. the difference of the surface charges may partly explain the "sorting-out" phenomenon in the mixed reaggregates ...19761010163
[age-dependent changes in cytophotometric properties of nuclear dna and histones in newts (triturus vulgaris and triturus cristatus)]. 1976976354
mechanisms of cell interaction during primary embryonic induction studied in transfilter experiments.the transmission mechanisms operative at different stages of neutralisation during primary embryonic induction of the newt triturus vulgaris were studied in experiments employing nuclepore filters placed between interactive tissue explants. the transmission time of the neuralising effect was determined with 0.2 mum nuclepore filter. in another series of experiments the transformation of neuralised ectoderm by archenteron roof mesoderm into other parts of the cns was studied. although sufficientl ...1976964517
cytochemical evidence for stage-specific changes of nuclear rna and nonhistone protein content during early development of triturus vulgaris.during early embryogenesis of triturus vulgaris, rna and nonhistone protein contents of neuroectoderm nuclei change with stage specifically. maximum values were found in the late gastrula after embryonic induction, and in the late neurula with the formation of the neural tube. the stage-specific increases of rna and nonhistone protein are correlated with a preceding increase of feulgen-dna content.19761253920
the terminal autonomic nervous system in the pancreas of newts. electron microscopic investigations using acetylcholinesterase and zinc iodide-osmium tetroxide reactions.the intrinsic innervation in the pancreas of newts triturus vulgaris, tr. montandoni and notophthalmus. all the investigated species display: cholinergic innervation better developed than adrenergic, no qualitative differences regarding the fine structure of nerve fibers and terminals as compared to that previously described in the pancreas or in other glands, axo-axonal contacts. their functional signification as peripheric synapses is suggested. frequently neuro-glandular junctions with or wit ...1975168313
[analysis of the cell cycle and dna synthesis in early embryonic tissues (urodela, triturus vulgaris)].1. during early embryogenesis (from blastula to late tailbud) oftriturus vulgaris the dna content of isolated nuclei from various regions was measured cytophotometrically. it became evident that the distribution of measurement data varies specifically with stage and region. 2. the nuclei were classified on the basis of their dna content into the appropriate phases of the cell cycle (g1 si, sii, siii, siv, and g2). the relative lengths of the cell cycle phases were calculated from the frequencies ...197428304718
the rejection of skin allografts in three populations of triturus vulgaris (l.). 19744611810
[studies on the problem of dna constancy during embryogenesis (urodela, triturus vulgaris)].1. during early embryogenesis (from morula to late tail-bud) of triturus vulgaris the dna content of isolated nuclei from various regions was measured cytophotometrically. the measurement data (50 to 70 for each region and developmental stage) were collated to so-called karyograms, which show the dna doubling during interphase and serve for determining the "normal" dna content (dna value of g1-nuclei). 2. thereby it became evident that the dna content of g1-nuclei is not constant in the course o ...197228304517
retinotectal projection in half eyes in the adult newt, triturus vulgaris. 19715559637
delayed termination of nuclear histone doubling after premeiotic dna synthesis in triturus vulgaris male meiosis. 19715145012
mosquito control based on bacillus thuringiensis israelensis (bti) interrupts artificial wetland food chains.the biocide bacillus thuringiensis israelensis (bti) has become the most commonly used larvicide to control mosquitoes in seasonal wetlands. although bti is considered non-toxic to most aquatic organisms, the non-biting chironomids show high susceptibilities towards bti. as chironomids are a key element in wetland food webs, major declines in their abundance could lead to indirect effects that may be passed through aquatic and terrestrial food chains. we conducted two mesocosm experiments to add ...201931412513
underwater sound production varies within not between species in sympatric newts.sound production is a widespread phenomenon among animals. effective sound use for mate or species recognition requires some acoustic differentiation at an individual or species level. several species of caudate amphibians produce underwater sounds, but information about intra- and interspecific variation in their acoustic production is missing. we examined individual, sex, and species variation in underwater sound production in adults of two sympatric newt taxa, ichthyosaura alpestris and lisso ...201930944780
[the statement of nuclear volume, dna content and nuclear form in tissue sections (author's transl)].15 days after amputation of the forelimb of young postmethamorphic triturus vulgaris dna-content and volume of the nuclei of the mesodermal blastema cells have been investigated in longitudinal sections of 2 microns in thickness by feulgen-photometric and electronic determination of area. discrimination of the dna-synthesis phase was carried out using microautoradiography after "in vivo" labeling with tritiated thymidine. volume and dna-content of the nuclear sections have been found to be posit ...1979295407
chromosome location of the ribosomal rna genes in triturus vulgaris meridionalis (amphibia, urodela).the mitotic chromosomes of six specimens from triturus vulgaris meridionalis have been examined by both in situ hybridization with 3h 18s + 28s rrna and as-sat staining method. the results of these two sets of experiments can be summarized as follows: 1) in each specimen the nors and the additional ribosomal sites, which react positively to in situ hybridization with 3h 18s + 28s rrna, are also stained by silver; 2) other chromosomal regions, which do not hybridize in situ with 3h 18s + 28s rrna ...197883935
surface topography of isolated gastrula ectoderm of triturus vulgaris.the surface topography of the outer cell layer of the two-layered gastrula ectoderm of triturus vulgaris resembles that of the other amphibians: the cells are tightly packed and devoid of pseudopodia. the cells of the inner layer are spherical and interconnected with pseudopodia of various lengths. after excision of the ectoderm the peripheral cells of the outer layer elongate perpendicularly to the edge of the explant, while the number of pseudopodia on the inner layer greatly increases. the re ...1978732362
an improved microsatellite panel to assess genetic variability of the italian smooth newt (lissotriton vulgaris meridionalis).habitat loss and fragmentation are threatening amphibians by increasing population isolation. however, artificial waterbodies created for livestockmay contrast this phenomenon by providing surrogate habitats for amphibians. here, we performed a genetic study on an amphibian species, lissotriton vulgaris meridionalis, in a rural area in central italy where natural wetlands are disappearing and drinking troughs for cattle watering are widespread. specifically, we tested a panel of microsatellite m ...201829932078
helminth endoparasites of the smooth newt lissotriton vulgaris: linking morphological identification and molecular data.the helminth endoparasites of many european amphibian species are often known exclusively from morphological descriptions. a molecular library of dna sequence data linked to morphological identifications is still in its infancy. in this paper, we aim to contribute to such a library on the smooth newt lissotriton vulgaris, the intermediate and definitive host of 31 helminth parasites, according to evidence published so far. newts (n = 69) were collected at two study sites in western germany and e ...201929502544
egg-laying environment modulates offspring responses to predation risk in an amphibian.predator-induced plasticity has been in the focus of evolutionary ecological research in the last decades, but the consequences of temporal variation in the presence of cues predicting offspring environment have remained controversial. this is partly due to the fact that the role of early environmental effects has scarcely been scrutinized in this context while also controlling for potential maternal effects. in this study, we investigated how past environmental conditions, that is different com ...201829485239
ontogeny of the hyobranchial apparatus in the salamanders ambystoma talpoideum (ambystomatidae) and notophthalmus viridescens (salamandridae): the ecological morphology of two neotenic strategies.comparison of metamorphosis of skull and hyobranchial system in two species of neotenic salamanders reveals two different types of neoteny. ambystoma talpoideum is completely neotenic owing to delayed metamorphosis. notophthalmus viridescens exhibits limited neoteny as a result of incomplete metamorphosis. morphological details of neoteny are compared to life history in both species in order to discuss the ecological morphology of the two neotenic strategies. comparisons to taricha granulosa, tr ...198729921111
the fine structure of the pigment epithelium and photoreceptor cells of the newt, triturus viridescens dorsalis (rafinesque).the morphology of the retinal pigment epithelium and photoreceptor cells has been studied in the common newt triturus viridescens dorsalis by light, conventional transmission and scanning electron microscopy. the pigment epithelium is formed by a single layer of low rectangular cells, separated by a multilayered membrane (bruch's membrane) from the vessels of the choriocapillaris. the scleral border of the pigment epithelium is highly infolded and each epithelial cell contains smooth endoplasmic ...197130373332
captivity and infection by the fungal pathogen batrachochytrium salamandrivorans perturb the amphibian skin microbiome.the emerging fungal pathogen, batrachochytrium salamandrivorans (bsal) is responsible for the catastrophic decline of european salamanders and poses a threat to amphibians globally. the amphibian skin microbiome can influence disease outcome for several host-pathogen systems, yet little is known of its role in bsal infection. in addition, many experimental in-vivo amphibian disease studies to date have relied on specimens that have been kept in captivity for long periods without considering the ...201931507541
differential introgression across newt hybrid zones: evidence from replicated transects.genomic heterogeneity of divergence between hybridizing species may reflect heterogeneity of introgression, but also processes unrelated to hybridization. heterogeneous introgression and its repeatability can be directly tested in natural hybrid zones by examining multiple transects. here, we studied hybrid zones between the european newts lissotriton montandoni and two lineages of lissotriton vulgaris, with replicate transects within each zone. over 1,000 nuclear genes located on a linkage map ...201931549466
common midwife toad ranaviruses replicate first in the oral cavity of smooth newts (lissotriton vulgaris) and show distinct strain-associated pathogenicity.ranavirus is the second most common infectious cause of amphibian mortality. these viruses affect caudates, an order in which information regarding ranavirus pathogenesis is scarce. in the netherlands, two strains (cmtv-nl i and iii) were suspected to possess distinct pathogenicity based on field data. to investigate susceptibility and disease progression in urodeles and determine differences in pathogenicity between strains, 45 adult smooth newts (lissotriton vulgaris) were challenged via bath ...201930872735
no trade-offs in interspecific interference ability and predation susceptibility in newt larvae.coexistence of species with similar requirements is allowed, among others, through trade-offs between competitive ability and other ecological traits. although interspecific competition is based on two mechanisms, exploitation of resources and physical interference, trade-off studies largely consider only species' ability to exploit resources. using a mesocosm experiment, we examined the trade-off between interference competition ability and susceptibility to predation in larvae of two newt spec ...201830271569
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