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a comparative cytochemical study of the yolk platelets in the growing oocytes of triturus cristatus and rana esculenta.recent cytochemical techniques for the detection of proteins, neutral and acid mucopolysaccharides, lipids and phospholipids in the yolk platelets of the growing oocytes of rana esculenta and triturus vulgaris are employed. cytochemical analysis showed that the yolk in the form of granules or platelets, in the constituting itself the most prominent cytoplasmic inclusion of amphibian eggs, contains a protein moiety consisting of individual typical amino acids lacking tryptophan in triturus, a car ...197773338
chromosome location of the ribosomal rna genes in triturus vulgaris meridionalis (amphibia, urodela).the mitotic chromosomes of six specimens from triturus vulgaris meridionalis have been examined by both in situ hybridization with 3h 18s + 28s rrna and as-sat staining method. the results of these two sets of experiments can be summarized as follows: 1) in each specimen the nors and the additional ribosomal sites, which react positively to in situ hybridization with 3h 18s + 28s rrna, are also stained by silver; 2) other chromosomal regions, which do not hybridize in situ with 3h 18s + 28s rrna ...197883935
[analysis of the structural features of the sperm chromatin of amphibia].deoxyribonucleoproteid (dnp) organisation of the sperm from amphibian species (rana temporaria, triturus vulgaris, bufo bufo) with different sperm chromosomal basic proteins has been analysed by nuclease fragmentation. electrophoretic distribution of chromatin fragments reveals regular organisation of dnp in the sperm of the species investigated. in spermal dnp of the frog rana temporaria, 3 sub-fractions of histone h1 were found. in the sperm of the newt triturus vulgaris and the toad bufo bufo ...1979116455
the terminal autonomic nervous system in the pancreas of newts. electron microscopic investigations using acetylcholinesterase and zinc iodide-osmium tetroxide reactions.the intrinsic innervation in the pancreas of newts triturus vulgaris, tr. montandoni and notophthalmus. all the investigated species display: cholinergic innervation better developed than adrenergic, no qualitative differences regarding the fine structure of nerve fibers and terminals as compared to that previously described in the pancreas or in other glands, axo-axonal contacts. their functional signification as peripheric synapses is suggested. frequently neuro-glandular junctions with or wit ...1975168313
towards a model of the courtship of the smooth newt triturus vulgaris, with special emphasis on problems of observatility in the simulation of behaviour. 1977194124
[the statement of nuclear volume, dna content and nuclear form in tissue sections (author's transl)].15 days after amputation of the forelimb of young postmethamorphic triturus vulgaris dna-content and volume of the nuclei of the mesodermal blastema cells have been investigated in longitudinal sections of 2 microns in thickness by feulgen-photometric and electronic determination of area. discrimination of the dna-synthesis phase was carried out using microautoradiography after "in vivo" labeling with tritiated thymidine. volume and dna-content of the nuclear sections have been found to be posit ...1979295407
early steroid metabolism in xenopus laevis, rana temporaria and triturus vulgaris embryos.embryos of xenopus laevis, rana temporaria and triturus vulgaris exposed to radioactive pregnenolone have been found to convert it to progesterone. incubations with radioactive progesterone showed that it was actively metabolized by oocytes and embryos. in xenopus incubations progesterone was converted to 5alpha-pregnane-3,20-dione, 17alpha-hydroxy-4-pregnen-3-one, 4-androstene-3,17-dione and 17alpha-20alpha-dihydroxy-4-pregne-3-one, indicating 5alpha-reductase, 17alpha-hydroxylase, 19-20-desmol ...1977303192
distribution and homologies of subunits in the ldh isoenzyme pattern of urodeles.1. the subunit distribution and homologies of ldh isoenzymes are investigated in the species triturus vulgaris, cristatus, alpestris and helveticus, and ambystoma mexicanum by means of immunoprecipitation and starch gel electrophoresis. 2. fresh tissue extracts contain the following patterns: (a) trit. vulgaris--two isoenzymes: the m4 and h4 tetramers. no hybrid formation is observed between h and m subunits. (b) trit. cristatus--two isoenzymes: the m4 and h4 tetramers. occasional hybrid formati ...1978318283
rejection of skin allografts in two geographically distant populations of triturus vulgaris (l.) and t. cristatus (laurenti). 1978361175
an experimental analysis of bivalent interlocking in spermatocytes of the new triturus vulgaris.interlocked bivalents at 1st meiotic metaphase are relatively uncommon in spermatocytes of the newt triturus vulgaris, but their frequency of occurrence can be significantly increased by subjecting newts to a 24-h heat shock. newt spermatocytes are sensitive to a heart shock at any stage between the end of premeiotic s and mid to late pachytene. the heat shock does not cause evidence desynapsis, nor does it significantly affect chiasma frequency; therefore the interlocked condition induced in sp ...1979479321
[3h-thymidine incorporation into the macrophages in the process of eye regeneration in adult tritons].changes in the number of labelled macrophages in the regenerating eye cavity of the adult common newts were studied within 2 to 12 days following the injection of 3h-thymidine and removal of retina and lens from the eyes. a small number of labelled macrophages was found in the eye cavity (0,2--5.9%) at all regeneration stages under study upon the pulse 3h-thymidine incorporation. their number rapidly increased and attained by the end of observation (12 days) 73%. these results suggest that mainl ...1979481852
tissue-specific changes in nuclear rna content during early development of triturus vulgaris.during early development of triturus vulgaris, as a measure for nuclear activity in neuroectoderm, mesoderm and endoderm, nuclear rna content was determined by cytochemical methods. in the first stages of gastrulation, that is to say during the early phase of neural induction, the rna content of the inducing system is considerably higher than in the reacting system. then, with a phase-shift of about 10 h, the rna content of the neuroectoderm increases quickly also. in the following stages the nu ...1979511597
scanning and transmission electron microscopy of intraventricular dendrite terminals of hypothalamic cerebrospinal fluid contacting neurons in triturus vulgaris.a scanning (sem) and transmission electron microscopic (tem) study of the ventricular wall of the hypothalamus of triturus vulgaris was performed with special regard to the intraventricular dendrite terminals of the cerebrospinal fluid (csf) contacting neurons of the preoptic area (magnocellular and parvocellular preoptic nuclei), the infundibular lobe (anterior periventricular nucleus, infundibular nucleus), and the paraventricular organ. in the preoptic area and infundibular lobe, the terminal ...1979524982
chromosome location of the ribosomal rna genes in triturus vulgaris meridionalis (amphibia, urodela). ii. intraspecific variability in number and position of the chromosome loci for 18s + 28s ribosomal rna.ribosomal genes have been localized on mitotic and lampbrush chromosomes of 20 specimens of triturus vulgaris meridionalis by in situ hybridization with 3h 18s + 28s rrna. the results may be summarized as follows: 1) each individual shows positive in situ hybridization at the nucleolus organizing region (nor) on chromosome xi; 2) in addition, many specimens exhibit a positive reaction in chromosomal sites other than the nor (additional ribosomal sites); 3) the chromosomal distribution of the add ...1977598264
chromosome location of the ribosomal genes in triturus vulgaris meridionalis (amphibia urodela). iii. inheritance of the chromosomal sites for 18s + 28s ribosomal rna.in triturus vulgaris meridionalis, the 18s + 28s rdna sequences have been shown to be located in a number of additional chromosomal sites besides the nucleolus organizing region. the additional ribosomal sites have been found to vary as to their number and chromosomal location in different individuals of the species.--the data presented in this study concern the chromosomal distribution of the ribosomal sequences as analyzed by in situ hybridization technique in two individuals as well as in the ...1978630997
[transfer of behavior patterns through transplantation of systems of neuroanatomic structures in amphibian larvae. ii. xenoplastic transplantation of hind brain systems between xenopus laevis (daud.) as well as hymenochirus boettgeri (torn.) and triturus vulgaris].by replacing parts of the neural plate (prospective medulla oblongata) of triturus vulgaris by neural ectoderm of xenopus laevis larval chimaeras were obtained, which showed donorlike rhythmical movements of distinct parts of the visceral musculature according to the place of implantation. especially the branchial region with its external gills showed rhythmical movements and is the special object of this investigation.1978654606
[late labelling of pigment epithelial and iridic cells in smooth newts].the late labelling of the actively proliferating cells of iris and pigment epithelium in the regenerating eye was found in the adult smooth newts within 2 to 12 days following the intraperitoneal injection of 3h-thymidine. this late labelling is due to the long-term presence of the labelled precursors of dna synthesis in the organism and to their reutilization. the index of labelled nuclei upon reutilization in the pigment epithelium is twice and in the iris 3 to 4 times higher than in the exper ...1978724207
surface topography of isolated gastrula ectoderm of triturus vulgaris.the surface topography of the outer cell layer of the two-layered gastrula ectoderm of triturus vulgaris resembles that of the other amphibians: the cells are tightly packed and devoid of pseudopodia. the cells of the inner layer are spherical and interconnected with pseudopodia of various lengths. after excision of the ectoderm the peripheral cells of the outer layer elongate perpendicularly to the edge of the explant, while the number of pseudopodia on the inner layer greatly increases. the re ...1978732362
mesodermalization of amphibian gastrula ectoderm in transfilter experiments.transfilter experiments were carried out with competent gastrula ectoderm of triturus vulgaris and ambystoma mexicanum as the reacting materials and blastoporal lip, alcohol-treated bone marrow and archenteron roof as the inducing materials, it was shown that a mesodermal inducing substance is able to penetrate nuclepore filters of different pore sizes (3.0, 1.0, 0.4, 0.2 micrometer). the degree of mesodermal differentiations depends on the reacting materials, the inductors and the filters which ...1978732366
the rejection of intra- and intersexual skin grafts in newts (triturus vulgaris and triturus alpestris). 1975764953
changes in the thermal denaturation profiles of dna from different developmental stages of the newt triturus vulgaris.the melting profiles of dna samples from the early gastrula and early neurula of triturus vulgaris are essentially the same, whereas dna from mid to late gastrula possesses higher tm values and shows a deviation from the regular sigmoidal shape at temperatures above tm. the plot on normal probability paper indicates a second dna fraction which melts at higher temperatures and, consequently, it has a higher gc-content than the bulk of dna. these facts confirm our idea that differential dna replic ...1977923733
mechanisms of cell interaction during primary embryonic induction studied in transfilter experiments.the transmission mechanisms operative at different stages of neutralisation during primary embryonic induction of the newt triturus vulgaris were studied in experiments employing nuclepore filters placed between interactive tissue explants. the transmission time of the neuralising effect was determined with 0.2 mum nuclepore filter. in another series of experiments the transformation of neuralised ectoderm by archenteron roof mesoderm into other parts of the cns was studied. although sufficientl ...1976964517
[age-dependent changes in cytophotometric properties of nuclear dna and histones in newts (triturus vulgaris and triturus cristatus)]. 1976976354
the surface charge of mesodermal and neural cells of triturus embryos.the electrical surface charge of disaggregated mesodermal and neural cells of the neurula stage of triturus vulgaris embryos is estimated by using the apparatus described in figure 1. the results showed a significant difference between the net charges of these two cell types: the neural cells had a clearly negative charge, whereas the mesodermal cells seemed to be more or less neutral. the difference of the surface charges may partly explain the "sorting-out" phenomenon in the mixed reaggregates ...19761010163
the structure of the alimentary canal and the dimensions of the mucosa in the smooth newt (triturus vulgaris l.). 19761016898
cytochemical evidence for stage-specific changes of nuclear rna and nonhistone protein content during early development of triturus vulgaris.during early embryogenesis of triturus vulgaris, rna and nonhistone protein contents of neuroectoderm nuclei change with stage specifically. maximum values were found in the late gastrula after embryonic induction, and in the late neurula with the formation of the neural tube. the stage-specific increases of rna and nonhistone protein are correlated with a preceding increase of feulgen-dna content.19761253920
ultrastructural observations on blood vessels surrounding normal and regenerating spinal cord in newt.the ultrastructural analysis of capillaries surrounding normal and regenerating caudal spinal cords of two species of newt (triturus vulgaris and t. cristatus) is reported. around normal spinal cords, capillaries were generally continuous. surrounding the regenerating spinal cords the various capillaries of the regenerating blastema appeared discontinuous with small or broad gaps along the capillary wall. this was seen even after two months of tail regeneration when the spinal cord was similar t ...20061303068
a newt ribozyme: a catalytic activity in search of a function.we analyzed the cleavage properties and the transcription regulation of the newt (triturus vulgaris meridionalis) self-cleaving rna. in vitro self-cleavage of model oligoribonucleotides occurs within a double hammerhead structure. in addition, an entire ribozyme molecule, as well as its catalytic domain, "trans-cleaves" in vitro appropriate oligoribonucleotide substrates. signals encoded within the ribozyme dna sequences regulate the ribozyme transcription, which is rna polymerase ii dependent. ...19921542657
two dispersed highly repeated dna families of triturus vulgaris meridionalis (amphibia, urodela) are widely conserved among salamandridae.two bamhi families of repeated sequences were characterized from the genome of the italian smooth newt, triturus vulgaris meridionalis (amphibia, urodela). the first family, which is divided into subfamilies, consists of tandemly arranged arrays whose basic repeat is around 398 bp long; these arrays are dispersed throughout the entire chromosome sets of the various species of triturus tested. moreover the family is widely conserved among salamandridae, being detected by genomic dna blotting of n ...19912009819
a record of gravid cestodes bothriocephalus claviceps (goeze, 1782) from the newts triturus vulgaris (l.) (amphibia). 20072227717
the influence of furosemide and co2+ on electrolyte and water transport in newt distal tubule and frog skin.the technique of stationary microperfusion of the early distal tubule used in experiments on male newts (triturus vulgaris) revealed the intratubular effect of cocl2 and furosemide on the ion transport. cocl2 in concentration 5 x 10(-4) m increased na and cl reabsorption, reduced ca, mg and k reabsorption, and increased (tfexp/tfo)in from 1.14 +/- 0.04 to 1.68 +/- 0.27 (p less than 0.001). cocl2 in concentration 5 x 10(-5) m enhanced na and cl reabsorption, without affecting transport of other i ...19862423960
thermal behaviour of a4 lactate dehydrogenase purified from the heterothermic and sympatric vertebrate species brook lamprey (lampetra planeri), tench (tenca tenca), smooth (triturus vulgaris) and alpine newt (triturus alpestris).1. the a4 lactate dehydrogenase isozyme was purified to homogeneity from the tissues of brook lamprey (lampetra planeri), tench (tenca tenca), smooth newt (triturus vulgaris) and alpine newt (t. alpestris). 2. these four species share their geographical distribution in the same freshwater habitats, often live together in the same station and two of them are congeneric. steady-state kinetic investigations have shown that: 3. km (apparent) for pyruvate vs. temperature and (apparent) product ki (py ...19892620488
differentiation of liquor contacting neurons in the regenerating spinal cord of the newt triturus.this paper reports a detailed electron microscopic study of the ventricular cells in the growing spinal cord during the first two months of tail regeneration in triturus vulgaris and t. cristatus. in the proliferating ependyma some cells undergo a neural differentiation in order to produce "cerebro-spinal-fluid-contacting-neurons (csfcns). these round or pear-like cells store dense core vesicles, 80-100 nm in diameter, and show less electron-density than ependymal elements. the stereociliar appa ...19892636002
[the determination of ectodermal derivatives in various species of amphibia].the process of labyrinth determination has been studied in amphibians (three urodela and seven anura species) using homoplastic transplantation of ear ectoderm, containing labyrinth material onto the abdominal wall of embryos of the same stage of development. the stage of appearance of organ-specific properties in ear ectoderm was determined and the increase of these properties in the course of development was observed. the frequency of ear vesicles formation, the level of their differentiation ...20082717137
cobalt-dependent stimulation of sodium transport in the amphibian skin and nephron.one to ten millimolar cocl2, when applied to the outer surface of the apical membrane of the frog skin (rana temporaria), reversibly increased the potential differences and short-circuit current. these observations suggest that co2+ may control the gating system of the sodium channel. in the kidney of the newt (triturus vulgaris), proximal reabsorption is increased under the influence of 0.5 mm cocl2 injected into the lumen. when cocl2 (0.5 mm) was injected into the lumen of the distal tubule of ...19862873934
are there insertions in the ribosomal dna of vertebrates?we have analysed the eco ri restriction pattern of rdna of the newt triturus vulgaris and of some other amphibian species by southern blotting and hybridization with nick-translated xenopus rdna prepared from the recombinant plasmids pxlr11 and pxlr12 (21). after hybridization with r11, the 28s coding fragments become visible in two bands, a prominent one of 5.3 kb and a weak band of 5.9 kb representing about 8% of the 28s genes. the evidence obtained so far by additional digestions with bam hi ...19852991856
molecular organization of ribosomal rna genes clustered at variable chromosomal sites in triturus vulgaris meridionalis (amphibia, urodela).the ribosomal rna genes of triturus vulgaris meridionalis (amphibia, urodela) show the peculiar feature of being clustered not only at the nucleolar organizer, present in the species at a definite chromosome location, but also at "additional ribosomal sites" which are highly variable in number and chromosomal distribution among individuals. the additional ribosomal sites are most often found at specific chromosome regions, such as telomeres, c-bands and centromeres, in virtually all the chromoso ...19853003361
length polymorphism in rdna indicates somatic alterations in the genome of triturus vulgaris.we have analyzed the ecori restriction pattern of ribosomal genes (rdna) isolated from several organs of single individuals of the newt triturus vulgaris by southern blotting and hybridization with corresponding xenopus probes (r11 and r12). using length polymorphism of rdna spacers as a molecular marker, it became evident that in individual newts the pattern of ribosomal genes is not always constant but varies between different tissues of the same animal suggesting the occurrence of genome alte ...19863007245
heterochromatic dna in triturus (amphibia, urodela). i. a satellite dna component of the pericentric c-bands.we have studied the structure, genome organization, chromosomal location, conservation across species and transcription on lampbrush chromosomes, of an at-rich satellite dna component of the newt, triturus vulgaris meridionalis. the satellite (sat g), originally isolated by gradient centrifugation, represents about 2% of the vulgaris genome and comprises a highly repetitive sequence family (hindiii family), whose monomers have been cloned. the repeat units are about 330 bp long, as measured on g ...19863013516
extra-ribosomal spacer sequences in triturus.we show that, in triturus vulgaris meridionalis, sequences homologous to the rdna "non-transcribed" spacer (nts) are clustered at chromosomal loci where they are not associated with 18 s or 28 s rdna genes: these sequences are referred to as the extra-ribosomal spacer sequences. genomic clones containing such extra-ribosomal spacer sequences have been isolated. as shown by restriction mapping, these clones appear to consist mostly of repetitive bamhi fragments that are, in turn, internally repet ...19883065517
heterochromatic dna in triturus (amphibia, urodela) ii. a centromeric satellite dna.the mspi family of highly repeated sequences is a centromeric satellite dna representing about 1% of the genome of the italian smooth newt, triturus vulgaris meridionalis. we have studied the structure, genomic organization, chromosomal localization and conservation across species of this family. mspi sequences are around 197 bp long, as shown by sequencing of three cloned units. the family is organized in large clusters of tandemly arrayed units, present at almost all the centromeres of t.v. me ...19883219917
[the electrolyte content of the body of insects, ascidians, fishes, amphibians and mammals and its dynamics in ontogeny].in animals in which sodium is a predominant cation of the blood serum or haemolymph (rat, newt triturus vulgaris, sturgeon acipenser güldenstädti, herring clupea pallasi, ascidian goniocarpa rustica), its content in the body amounts up to a half of the total bulk of electrolytes; in insects with potassium type of the haemolymph, the share of sodium is equal to 6.4%. in animals of the same species, total electrolyte content (sodium, potassium, calcium, magnesium) and the relationship between sepa ...19883245357
protein synthesis in the mesodermal tissue of forelimb regenerates of triturus vulgaris: an autoradiographic study. 19883412340
[effect of catecholamines on electrolyte and water transport in the nephron of lower vertebrates].micropuncture technique and electron microprobe analysis have been used to investigate the role of noradrenalin in ion and water balance in the renal tubules of the lamprey lampetra fluviatilis and newt triturus vulgaris. noradrenalin decreased na, k, and ca concentrations in the proximal lumen of the lamprey increasing the value of (tf/p)in from 1.1 +/- 0.1 to 1.3 +/- 0.1 (p less than 0.001). regitin blocked these effects. noradrenalin perfusion of the peritubular capillaries in newt kidney inc ...20133618016
induction of ocular tumor by nickel subsulfide in the japanese common newt, cynops pyrrhogaster.chemically induced ocular tumors in amphibians have not been reported previously. in the present study, nickel subsulfide (ni3s2) was administered to lentectomized japanese common newts, cynops pyrrhogaster, by a single injection into the posterior chamber of the right eye (40-100 micrograms ni3s2/newt). control newts received a similar injection of vehicle. malignant melanoma-like tumors developed in the injected eyes of seven of eight ni3s2-treated newts at 9 months (versus none of six control ...19873621207
[capillaria tritonispunctati (diesing, 1851) travassos, 1915 and megalobatrachonema terdentarum (linstow, 1890) hartwich, 1960 (nematoda) in newts (triturus vulgaris l. and triturus cristatus (laur.))]. 19873687016
the effects of two retinoids on limb regeneration in pleurodeles waltl and triturus vulgaris.the effects of two vitamin a analogues, retinol palmitate and retinoic acid, on pattern formation during limb regeneration in larvae of two european newts, p. waltl and t. vulgaris are described. the response of the regenerating limb to retinoid treatment differed according to the larval stage of development for p. waltl. young larval limbs, which were anterior limb buds at the time of amputation, duplicated transversely while limbs of older larvae duplicated proximodistally. proximodistal dupli ...20133723061
effect of concanavalin a and vegetalizing factor on the outer and inner ectoderm layers of early gastrulae of xenopus laevis after treatment with cytochalasin b.neural (archencephalic) structures have been evoked in the competent ectoderm (consisting of both ectodermal layers) of xenopus laevis by treatment with concanavalin a (con a), which probably acts on the plasma membrane. the size of the neural structures is increased when the ectoderm is incubated in cytochalasin b prior to the con a treatment. the results indicate that cytochalasin b could have an influence on the binding of con a to receptors on the plasma membrane. on the other hand, cytochal ...19854005955
[ultrastructure of the kidney of the newt].in the newt [triturus vulgaris (j.)] kidney a zone of single nephrons is distinguished, where there are ten nephrons, connected with the paramesonephral (müller's) canal, and another zone of the renal mass; in the latter the main part of nephrons is situated, which are connected with the ureter by means of collecting tubules. in these zones of the kidney ultrastructure of epitheliocytes in the proximal and distal parts is different. the renal corpuscles of the epithelial cell in the cervical and ...19854051783
[furosemide-sensitive transport of chloride through the apical membrane of cells of the proximal tubule of the triton kidney].the effect of furosemide on transtubular electrical potential (vt) as well as on the potentials of apical (va) and basolateral (vb) membranes of the triturus vulgaris proximal nephron cells was studied in alteration of the electrochemical potential gradient na+ and cl- through the apical membrane. furosemide did not change the va and vb provided the concentration of nacl in the lumen of proximal canal was the same as in the blood plasma. when the nacl concentration diminished in the lumen furose ...20134065369
[annual cyclic studies of catecholamines in the hypothalamus of triturus vulgaris (l.)]. 19734207950
the rejection of skin allografts in three populations of triturus vulgaris (l.). 19744611810
ultrastructural studies of oogenesis in some european amphibians. ii. triturus vulgaris. 19734766454
the control of alternating coordination of limb pairs in the newt (triturus vulgaris). 19734789785
contribution to study of the arterial supply of the gastrointestinal tract of the newt (triturus vulgaris). 19734798659
ultrastructural alterations in the pancreatic b-cells of the newt (triturus vulgaris) induced by alloxan. 19714939836
delayed termination of nuclear histone doubling after premeiotic dna synthesis in triturus vulgaris male meiosis. 19715145012
[cytochemical demonstration of phase- and region-specific changes in dna content during the embryogenesis of triturus vulgaris]. 19695365872
[cytophotometric study of the non-homogeneity of the cell nucleus during the process of differentiation in the mesoderm of triturus vulgaris]. 19705472238
spontaneous aberrations in lampbrush chromosome xi from a specimen of triturus vulgaris meridionalis (amphibia, urodela). 19705474809
the maps of the lampbrush chromosomes of triturus (amphibia urodela). iv. triturus vulgaris meridionalis. 19705489360
retinotectal projection in half eyes in the adult newt, triturus vulgaris. 19715559637
a radioautographic study of the time course of male meiosis in the newt triturus vulgaris. 19685707855
plagitura intermedia sp. n. (trematoda: plagiorchiidae) from the common newt, notophthalmus viridescens viridescens. 19655857275
limitations in response capacity of the newt, notophthalmus viridescens, to soluble and particulate antigens.we have investigated the cellular basis of the failure of primitive extant vertebrates, e.g. notophthalmus viridescens, the american common newt, to respond to soluble thymus-dependent (td) antigens, e.g. keyhole limpet hemocyanin (klh). we have found that klh can be used to prime for amplification of a response to hapten, 2,4,6-trinitrophenyl (tnp) when it is conjugated to klh, only if both the unconjugated and conjugated klh are adsorbed onto bentonite particles. the response is monitored in t ...19826182036
single channel ion currents in embryonic cells of the newt (triturus vulgaris) isolated without enzymatic treatment. 19836318520
[radioautographic study of nonhistone protein synthesis in the process of lens and retina regeneration in adult tritons].the incorporation of 3h-tryptophane (3h-t) in the cell nuclei of pigment epithelium, retinal rudiment, iris and vitreous body has been studied during lens and retina regeneration in the adult smooth newts. a gradual increase (7 to 13-fold as compared with the control) of the intensity of labelling was found in the cell nuclei of pigment epithelium of the central part of fundus oculi. the cells of this zone are a source of regeneration. in the peripheral zones which do not participate in retina r ...20156621999
[microscopic anatomy of the triton kidney].the structure of the nephron, length of all its segments and the renal architectonics as a whole have been studied in the newt (triturus vulgaris l.) by means of microdissection and microinjection methods. the microinjection of latex or lissamine green is performed into the blood vessels and nephrons. in one kidney 85-95 nephrons are counted, their number is not the same along the kidney length and increases in the caudal direction. there are nephrons of the ventral, intrarenal and medial popula ...19846712491
[relative characteristics of the duration of embryonic development in triturus vulgaris].the data of valouch et al. (1971) on tau 0 values in the smooth newt at different temperatures are presented as a curve. using this curve, the absolute (in hours and days) duration of successive developmental stages provided by glaesner (1925) is transformed in a relative one, expressed in the number of tau 0 (tau n/tau 0).20066739036
h-y antigen as a tool for the determination of the heterogametic sex in amphibia.h-y antigen was investigated in three amphibian species with different degrees of sex-chromosome differentiation: bufo bufo, triturus vulgaris, and pyxicephalus adspersus. no heteromorphic sex chromosomes were found in b. bufo, but an examination of the progeny of hermaphrodites (ponse, 1942) indicated that the female of this species was heterogametic (zw). sex chromosomes differing only by a very small heterochromatic region at their telomeres were found in the male of t. vulgaris (xy). pyxicep ...19816794989
[histochemical study of the cholinesterases of various formations of the vertebrate endbrain].histochemical studies have been made on true (ache) and false (bche) cholinesterases in different parts of the telencephalon of amphibians, reptiles and mammals. the data obtained indicate that the enzymic localization depends on both the level of the development of the brain and phylogenetic age of its individual formations. in the telencephalon of ambystoma mexicanum, high concentration of ache was found in vascular-capillary network, in other investigated amphibians (triturus vulgaris, rana t ...19816976662
the photoreceptor cell in the pineal organ of the japanese common newt. 19827151960
the activity of the nucleus preopticus during limb regeneration in adult newts, triturus vulgaris. 19817262354
[microperfusion and electrophysiologic study of the proximal tubules of triturus vulgaris and rat kidneys].studies have been made on changes in na and k concentrations in the lumen of the proximal tubule of the kidney of rat and newt during perfusion of the tubuli by solutions containing various concentrations of these ions. intraluminar concentration of na and k is rapidly changed and towards the end of the perfused region the ratio of na and k concentration in the lumen to the concentration in the blood plasma approaches 1. within the lumen of the perfused proximal tubuli of newt's kidney, na and k ...19807424296
qualitative changes in dna indicating differential dna replication during early embryogenesis of the newt triturus vulgaris.during gastrulation of the newt triturus vulgaris considerable changes in the melting behaviour and in the cscl density gradient pattern of dna occur. the melting curves of dnas from mid to late gastrulae (stages 11b-12c) deviate from the regular shape. whereas the tm values are identical in the stages 11a (early gastrula) and 15 (early neurula), and correspond to the standard dna (stage 36 = tailbud), a significant rise of tm (0.8-1.2 degrees c) has been recorded in the stages 12a/b (yolk plug) ...19807430936
activity of pars tuberalis of the hypophysis during limb regeneration in adult triturus vulgaris. 19807450089
comparison of the pineal complex, retina and cerebrospinal fluid contacting neurons by immunocytochemical antirhodopsin reaction.the presence of rhodopsin was investigated by an indirect immunocytochemical method in the pineal complex of various vertebrates (carassius auratus, cyprinus carpio, hypophthalamichthys molitrix, lucioperca lucioperca, triturus vulgaris, bombina bombina, rana esculenta, pseudemys scripta elegans, lacerta agilis et viridis, white leghorn chickens, rat), in the retina of lebistes reticulatus, lucioperca lucioperca, rana esculenta, lacerta agilis, pseudemys scripta elegans, the chicken and the rat, ...19807456628
expression of alpha sm actin in terrestrial ectothermic vertebrates.alpha-smooth muscle (alpha sm) actin of endothermic vertebrates is selectively recognized by the monoclonal antibody anti-alpha sm-1. immunoreactivity to this antibody has been shown to be localized in the nh2-terminal sequence ac-eeed (chaponnier et al. 1994). among terrestrial ectothermic vertebrates, two amphibian (triturus vulgaris, rana esculenta) and three reptilian species (pseudemys scripta elegans, natrix natrix, podarcis sicula) were screened to investigate if their vascular and viscer ...19957553770
blood supply to the amphibian mesonephros.the purpose of the present study was to describe geometry of branching, topography and sources of blood vessels supplying the mesonephros of three different species of amphibia and to infere from the results their common and species-specific features. the detailed information on arrangement of portal and arterial blood supply to mesonephros is necessary for better understanding of the conditions under which these kidneys function. for this reason the blood vessels of the mesonephros of adult hyn ...19937949400
spinal cord neuron classes in embryos of the smooth newt triturus vulgaris: a horseradish peroxidase and immunocytochemical study.spinal cord neurons were investigated in embryos of triturus vulgaris, the smooth newt, just prior to hatching. these embryos can swim if freed from their egg membranes. horseradish peroxidase (hrp) labelling, together with gaba and glycine immunocytochemistry (icc), revealed nine distinct anatomical classes of neuron. 1. ventrolateral motoneurons with mainly dorsal dendrites, sometimes a descending central axon and peripheral axon innervating the trunk muscles. 2. dorsal primary sensory rohon-b ...19938099742
unilateral forelimb amputation affects protein synthesis in ipsilateral and contralateral spinal cord and spinal ganglia neurons of the newt in vivo.the aim of this investigation was to analyze the effect of urodele limb amputation and blastema growth on the protein synthesis in spinal cord and spinal ganglia neurons corresponding to the 3rd and 4th spinal segment of young, postmetamorphic triturus vulgaris, in vivo. protein synthesis was studied on both trunk sides as a function of right forelimb amputation at the distal stylopodium, and blastema development, during a critical period of 22 days post amputation (dpa). protein synthesis was a ...19938168437
modulation of the effect of arginine-vasopressin on water and ion transport in the newt early distal tubule and frog urinary bladder by v1-antagonists.in the early distal tubule of the newt triturus vulgaris l., 1 nm arginine-vasopressin (avp) increased water reabsorption; the fractional reabsorption of na+ was elevated from 46.2 +/- 6.9% to 67.8 +/- 3.9% (p < 0.001), of cl- from 52.7 +/- 6.7% to 73.1 +/- 3.5% (p < 0.001), of mg2+ from 48.0 +/- 7.7% to 71.7 +/- 6.3% (p < 0.001). when v1-receptors were blocked by 1 nm peptide v1-antagonist [1-(beta-mercapto-beta,beta-cyclopentamethylene propionic acid), 2-(o-methyl) tyr]-[arg8]vasopressin, 1 nm ...19958594534
similar localization of immunoreactive glutamate and aspartate in the pineal organ and retina of various nonmammalian vertebrates.the localization of immunoreactive glutamate and aspartate was compared in the pineal organ and retina of various vertebrates (raja clavata, carassius auratus, salvelinus alpinus, triturus vulgaris, triturus cristatus, lacerta muralis, lacerta agilis, lacerta viridis, columbia livia and white leghorn chicken) by postembedding immunoelectron microscopy. immunoreaction of both excitatory amino acids was detected in the pinealocytes in a localization similar to that of retinal photoreceptors. the r ...19958714767
local effects of glycinergic inhibition in the spinal cord motor systems for swimming in amphibian embryos.1. we have studied the effects of locally applying the glycinergic antagonist strychnine to rhythmically active spinal neurons in amphibian embryos during fictive swimming. intracellular recordings were made from motoneurons and premotor interneurons in xenopus laevis, a well-studied model system, and from motoneurons in three other species (rana temporaria, bufo bufo, and triturus vulgaris). overall, these embryos cover a range of swimming patterns from the short-cycle-period, brief-motor-root ...19968871217
asymmetrical turning during spermatophore transfer in the male smooth newtduring mating, male smooth newts, triturus vulgarisshowed a population bias for turning left during spermatophore transfer. this is the first demonstration of asymmetrical sexual behaviour in an amphibian population. an experiment with a perfect female model showed that it was due to male rather than female lateralization. left turning bias decreased progressively as more spermatophores were deposited, and no significant bias was detectable by the third deposition. the hypothesis that this was d ...19979268466
molecular structure of the rdna intergenic spacer (igs) in triturus: implications for the hypervariability of rdna loci.ribosomal dna (rdna) variation in the species triturus vulgaris meridionalis (amphibia, urodela) is remarkable because of unusually high intraspecific variability in the number and distribution of ribosomal loci in the karyotype; in addition, portions of the intergenic spacer (igs) are clustered at chromosomal loci where they are not associated with ribosomal 18s and 28s rna genes. these clusters are referred to as extraribosomal, and they appear to consist mostly of repetitive bamhi elements. i ...19979297510
lithium secretion in kidneys of amphibians and reptiles under hydrated conditions.renal lithium transport was studied at different hydration levels in five species of anuran amphibians (bufo bufo, b. danatensis, b. viridis, rana ridibunda, and r. temporaria), two species of urodeles (triturus vulgaris and t. cristatus) and four species of reptiles (lizards eremias multiocellata, lacerta vivipara, trapelus sanguinolentus, and teratoscincus scincus). under dehydration conditions, li+ was reabsorbed in the kidneys of amphibians ans reptiles, but to a lesser degree than in mammal ...19979505431
[study of amphibian brain asymmetry during normal embryonic and larval development].amphibian embryos (triturus vulgaris, pleurodeles waltl, hynobius keyserlingii, ambystoma mexicanum, rana temporaria, bufo bufo, and xenopus laevis) were investigated. we ascertained the morphological right-side asymmetry of the brain, which appears at the neurula stage and is preserved during the postembryonic (larval) development. in t. vulgaris, p. waltl, h. keyserlingii, and x. laevis, we observed right-side asymmetry of peripheral analyzers, such as the retina, ganglia of nerve viii, and ol ...19989644914
comparative analysis of gnrh neuronal systems in the amphibian brain.we have investigated the gnrh-ir neuronal systems in the brain of the oviparous urodele, triturus vulgaris, ovoviviparous urodele, salamandra salamandra, and viviparous caecilian, typhlonectes compressicauda, and have reexamined xenopus laevis, ambystoma mexicanum, and rana esculenta. results showed that mgnrh neuronal system was diffused along the medioventral telencephalon and diencephalon with the numerical preponderance of gnrh cell bodies in the rostral mediobasal telencephalon in t. vulgar ...19989843639
amphibians and larvae of aquatic insects as new paratenic hosts of anguillicola crassus (nematoda: dracunculoidea), a swimbladder parasite of eels.amphibians (tadpoles of the frog bombina bombina [l.] and the newt triturus vulgaris [l.]) and aquatic insects (larvae of the alderfly sialis lutaria [l.] [megaloptera], dragonflies sympetrum sanguineum [miller] and coenagrion puella [l.] [odonata], and the caddisfly oligotrichia striati [l.] [trichoptera]) were found to serve as paratenic hosts for the third-stage larvae (l3) of the nematode anguillicola crassus kuwahara, niimi et itagaki, 1974, a pathogenic swimbladder parasite of the eel angu ...19989925427
sperm storage in females of the smooth newt (triturus v. vulgaris l.): i. ultrastructure of the spermathecae during the breeding season.sperm storage in cloacal spermathecae was studied in females of triturus v. vulgaris collected early in the breeding season in southern england. females collected in terrestrial situations, presumably unmated, were mated in the laboratory, and the ultrastructure of the transferred sperm and the spermathecae was observed at various intervals after mating. sperm from a spermatophore cap lodged in a female's cloacal orifice can migrate into spermathecae within 1 hr after mating. spherical structure ...19999990737
lectin binding patterns in amphibian skin epithelium.seven lectins (pna, dba, wga, uea-i, rca, sba, con a) were used to localize glycoconjugates in the skin of 10 species of amphibia, 7 anurans (bufo marinus, bufo bufo, rana ridibunda, rana pipiens, hyla arborea, pelobates syriacus and xenopus laevis) and 3 urodeles (salamandra salamandra, triturus vulgaris and ambystoma mexicanum). it was found that every lectin has a specific binding pattern in the skin of each species. no common pattern could be established, either among frogs or toads, nor for ...199910443294
paedogenesis in european newts (triturus: salamandridae): cranial morphology during ontogeny.a cross-sectional analysis using different ontogenetic stages (larvae, juveniles, paedotypic, and metamorphic adults) of the smooth newt, triturus vulgaris, and the alpine newt, t.alpestris, revealed a broad spectrum of perennibranchiation influences on cranial ontogeny in european newts, more pronounced than previously thought. these influences included marked variation in ossification levels, pronounced morphometric variability of many cranial elements, and considerable skull shape changes in ...200010658197
myotomal myogenesis in triturus vulgaris l. (urodela) with special reference to the role of mesenchymal cells.two stages can be distinguished in the differentiation of myotomal muscle fibres in triturus vulgaris. in the first stage only synchronously differentiating myotomal cells are engaged; in the second stage mesenchymal cells also take part in the process. myotomal cells (primary myoblasts) fuse to form 2-3 nucleate myotubes. only in the caudal part of the embryo mononucleate myotubes persist. the mononucleate myotubes, like polynucleate ones, occupy the whole length of the myotome. the differentia ...200011080917
distribution of blue-sensitive photoreceptors in amphibian retinas.previously, we reported that an opsin (rc-ms) belonging to the sws2 group opsins is expressed in bullfrog green rods [hisatomi, o. et al., febs lett., 1999, 447, 44-48]. an anti-rc-ms antiserum recognized the cones of the japanese common newt, cynops pyrrhogaster, which has no green rods. we isolated a cdna encoding an sws2 group opsin (cp-sws2) from this newt and found that cp-sws2 is expressed in a small population of the cones. our results suggest that sws2 opsins can be expressed in either g ...200111470275
influence of longitudinal whole animal clinorotation on lens, tail, and limb regeneration in urodeles.two species of newts (urodela) and two types of clinostats for fast clinorotation (60 rpm) were used to investigate the influence of simulated weightlessness on regeneration and to compare results obtained with data from spaceflight experiments. seven or fourteen days of weightlessness in russian biosatellites caused acceleration of lens and limb regeneration by an increase in cell proliferation, differentiation, and rate of morphogenesis in comparison with ground controls. after a comparable ti ...199611538637
influence of clinorotation and fettering stress on tail regeneration of triturus vulgaris (urodela).tail-amputated adult triturus vulgaris, fettered in cuvettes of a fast-rotating clinostat were exposed to simulated weightlessness (60 rpm; equiv. to 10(-3)-10(-4) g), during a 14-day period. to feed and clean the animals rotation was stopped once a day for approx. 10 min. to test the influence of the fettering stress, a second series of animals was kept separately under normal earth conditions without rotation. a further control series was kept in a dark container without any handicap. while ta ...199811541367
genetic differentiation of the two subspecies of the smooth newt inhabiting romania, triturus vulgaris vulgaris and t.v. ampelensis (urodela, salamandridae) as revealed by enzyme electrophoresis.starch gel electrophoresis was applied to quantify genetic variation and divergence in samples from romanian populations of the nominal form of the smooth newt triturus vulgaris and those of the endemic romanian subspecies t.v. ampelensis, a population from a parapatric area was additionally included. all the samples had similar levels of genetic variation measured by the mean heterozygosity, proportion of polymorphic loci, and mean number of alleles per locus. t.v. ampelensis samples were genet ...200111987463
opsin expression in adult, developing, and regenerating newt retinas.japanese common newts (cynops pyrrhogaster) have an ability to regenerate their neural retina even as adults. although extensive research has been carried out attempting to understand this retinal regeneration, the molecules characterized in newt retina are limited. we isolated cdnas encoding three putative opsins (cp-rh, -lws and -sws1), in addition to cp-sws2 [takahashi et al., febs lett. 501 (2001) 151-155] from a cdna library of adult newt retina. our immunohistochemical and in situ hybridiz ...200212106689
characteristics of development and ossification of skeletal elements in the limbs of the smooth newt triturus vulgaris (caudata). 201712577639
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