Publications

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[a case of cryptobia (trypanoplasma) infection in the blood of ctenopharyngodon idella val. bred in the carp farm ponds]. 19675625436
efficiency of grass carp (ctenopharyngodon idella val.) in controlling submerged water weeds. 197016057556
studies on gill parasitosis of the grasscarp (ctenopharyngodon idella) caused by dactylogyrus lamellatus achmerow, 1952. ii. epizootiology. 19715152840
studies on gill parasitosis of the grasscarp (ctenopharyngodon idella) caused by dactylogyrus lamellatus achmerow, 1952. 3. therapy and control. 19715152841
studies on gill parasitosis of the grasscarp (ctenopharyngodon idella) caused by dactylogyrus lamellatus achmerow, 1952. i. morphology and biology of dactylogyrus lamellatus. 19715168591
studies on gill parasitosis of the grasscarp (ctenopharyngodon idella) caused by dactylogyrus lamellatus achmerov, 1952. 19724669290
a rhabdovirus isolated from grass carp (ctenopharyngodon idella val.). 19751147780
[triploidy in hybrids of carp with other representatives of the cyprinidae family].as a result of remote hybridization of carp (cyprinus carpio l.) with the representatives of certain other subfamilies of cyprinidae a few viable f1 hybrids were obtained for the first time. these hybrids proved to be allotriploids. in the cross cyprinus carpio l. female x hemiculter eigenmanni (jord. et metz.) male the rate of survival of this year's brood was 0,5% of the fertilized eggs and 3n=128; whereas in the cross cyprinus carpio l. male x ctenopharyngodon idella (val.) female the rate of ...19751218729
parasitic ciliata at the ctenopharyngodon idella val., hypophthalmichthys molitrix val. and aristichthys nobilis rich. in ponds. 1976827857
the ultrastructure and renewal of the intestinal epithelium of the juvenile grasscarp, ctenopharyngodon idella (val.).the intestinal absorptive epithelium of starved and fed fish has been studied electron microscopically. after feeding, cells of the proximal segment of the intestine show morphological characteristics of lipid absorption. absorptive cells in the middle segment contain many pinocytotic vesicles in both fasted and fed specimens. absorption of protein macromolecules is supposed to be one of the main functions of this part of the gut. in the most caudal part of the intestine, absorptive cells carry ...1978630592
histomorphological and histochemical researches on the digestive tract of the freshwater grass carp, ctenopharyngodon idella (cypriniformes).ctenopharyngodon idella does not have a stomach, as is shown by the absence of gastric glands. the intestine is a simple coiled tube about twice the length of the body, having no valves and no ceca, and it is joined directly to the esophagus. the mucosa is fairly uniform in appearance, being thrown into folds which give it a mesh-like appearance. no multicellular glands can be detected in any part of the alimentary canal. goblet cells are present: no differences are found in young specimens. muc ...1979474989
regional functional differentiation in the gut of the grasscarp, ctenopharyngodon idella (val.).a regional differentiation--reflecting structural differences--of the intestine of larval and juvenile grasscarps can be illustrated by studying the activity of alkaline phosphatase and the uptake of orally administered horseradish peroxidase. pinocytosis takes place in a welldefined area of about 23% of the length of the gut (segment ii). neither the rostral +/- 68% (segment i) nor the caudal +/- 9% (segment iii) shows absorption of the enzyme. alkaline phosphatase activity, mainly localized at ...1979521316
[occurrence of bothriocephalosis in grass carp (ctenopharyngodon idella) in switzerland]. 19817221537
hematologic and enzymatic analysis of ctenopharyngodon idella x hypophthalmichthys nobilis f1 hybrids.1. erythrocyte counts, hemoglobin concentrations and hematocrit values were determined for diploid and triploid ctenopharyngodon idella x hypophthalmichthys nobilis hybrids and the parental species. 2. comparisons of diploid and triploid hybrids with the parental species revealed low erythrocyte counts for triploids, high mean corpuscular hemoglobin values for triploids, elevated hematocrits for diploids and triploids and similar hemoglobin concentrations for all fish. 3. alkaline phosphatase, a ...19836132739
changes in chromatin basic proteins during male gametogenesis of grass carp.1. the composition of basic of chromatin protein fractions has been studied in the course of male spermatogenesis of grass carp (ctenopharyngodon idella). 2. electrophoretic mobility of h1, h2b and h2a histones of grass carp is shown to differ from that of corresponding fraction of calf thymus histones. 3. comparison of chromatin protein fractions from liver, mature and immature testis and sperm of grass carp has revealed subtle tissue specificity of h2b and h2a histones. 4. maturation of sperma ...19836187516
[cataract due to worms (eye diplostomatosis) in the grassfish (ctenopharyngodon idella)]. 19836857640
partial characterization of transferrins of some species of the family cyprinidae.1. transferrins of five cyprinid fishes (tinca tinca, ctenopharyngodon idella, chondrostoma nasus. hypophthalmichthys molitrix and aristichthys nobilis) were isolated in a pure state using sephadex g-100, deae-sephadex and sp-sephadex. 2. all the transferrins are heterogeneous. in h. molitrix and a. nobilis the heterogeneity is caused partly, but not exclusively, by the presence of sialic acid; in the other species it is independent of sialic acid. 3. molecular weights ranging from 69,900 (t. ti ...19836839722
immunohistochemical localization of urotensin i and other neuropeptides in the caudal neurosecretory system of three species of teleosts and two species of elasmobranchs.in three species of teleosts - carp cyprinus carpio; grass carp ctenopharyngodon idella; and crucian carp carassius auratus - the caudal neurosecretory system displays small, medium-sized and large neurons. urotensin i (ui)-immunoreactive and ui-nonreactive neurons were found in all three groups; in general, the number of the latter neurons exceeded that of the former. noteworthy are: (i) ui-immunoreactive fibers in the caudal spinal cord and (ii) dense accumulations of ui-immunoreactive product ...19862424610
chemical control of some protozoan ectoparasites on young stages of the grass carp, ctenopharyngodon idella val. 19863097171
monooxygenase activity of fish liver in biomonitoring aquatic environment.the hepatic biotransformation activity of four cultivated freshwater fish, amur carp (ctenopharyngodon idella), carp (cyprinus carpio), rainbow trout (salmo gairdneri) and tench (tinca tinca) were studied one day after catching the fish from ponds and a river in the south of gdr and in fish kept for two weeks in clean filtrated and deacidified water. aryl hydrocarbon hydroxylase, 7-ethoxycoumarin o-deethylase and 7-ethoxyresorufin o-deethylase activities fell to about 20% during the stay in clea ...19863492989
cerebrospinal fluid-contacting neurons, ciliated perikarya and "peptidergic" synapses in the magnocellular preoptic nucleus of teleostean fishes.the magnocellular preoptic nucleus of fishes (anguilla anguilla, amiurus nebulosus, cyprinus carpio, carassius auratus, ctenopharyngodon idella, cichlasoma nigrofasciatum) has been studied by light and electron microscopy. two kinds of neurons were found: a) large, electron-dense, gomori-positive cells with moderate acetylcholinesterase (ache) positivity which contain granulated vesicles of 1400 to 2200 a (in average 1600 to 1800 a), adn b) small, strongly ache-positive, electron-lucent neurons ...1987174818
the beta-actin gene of carp (ctenopharyngodon idella). 19892762162
weedivorous fishes for the control of vectors of malayan filariasis.an attempt was made to assess the efficacy of phytophagous fishes viz., ctenopharyngodon idella, the chinese grass carp and osphronemus goramy, the giant gourami in the control of mansonioides mosquitoes by checking the growth of aquatic weeds which support their breeding. when c. idella and o. goramy were used in monoculture 80.21 and 81.25 per cent of the ponds were kept free from weeds. with polyculture using grass carp in combination with other varieties of carps, only 70.10 per cent of the ...19902269511
fish cell lines: establishment and characterization of three new cell lines from grass carp (ctenopharyngodon idella).three new cell lines were established from tissues of the grass carp, ctenopharyngodon idella. derived from the fin, snout, and swim bladder of two apparently healthy diploid fry, these cell lines have been designated gcf, gcs-2, and gcsb, respectively. the cells grew at temperatures between 24 degrees and 36 degrees c with optimal growth at 32 degrees c and have been subcultured more than 50 times since their initiation in august 1986. two of the lines remained diploid or pseudodiploid after 38 ...19902108122
the advantages of mosquito biocontrol by stocking edible fish in rice paddies.edible fish stocked in rice fields at a density of 600-800 fry per mu (1 mu = 1/15 hectare) for 150-170 days may act as an effective mosquito biocontrol agent. common carp (cyprinus carpio), grass carp ctenopharyngodon idella) and tilopia spp. killed late stage larvae and pupae of anopheles sinensis and culex tritaeniorrhyncus in laboratory and field trials. stocking of fish in experimental rice fields decreased larval numbers significantly in comparison with control areas. expansion of fish sto ...19911818398
[the seasonal dynamics of the celozoic stages of chloromyxum barbi (myxosporidia) in hypophthalmichthys molitrix (osteichthyes)].the seasonal dynamics of c. barbi of two consecutive generations of one-summer old silver carps (hypophthalmichthys molitrix) from a winter basin was investigated. one maximum of intensity in spring (80 to 100%) and one minimum in summer to early autumn (20% to 0) shows a course of incidence with a pattern of a one-year-cycle of the patent (celozoic) infection in both silver carp populations. c. barbi was also observed in grass carp (ctenopharyngodon idella) and bighead carp (aristichthys nobili ...19912039088
a cost benefit analysis of fish culture strategy towards the control of mansonioides in shertallai, kerala state.the cost benefit analysis of fish culture as a means for vector control towards the containment of brugian filariasis in shertallai, a highly endemic tract for this disease in kerala was carried out. while the financial gain accrued to the community was to the tune of rs. 1,82,052 in ponds (under monoculture) with chinese grass carp from 763 ponds, it was rs. 1,83,740 from 1000 ponds under polyculture with catla catla, cyprinus carpio, labeo rohita, labeo fimbriatus, cirrhina mrigala and ctenoph ...19921506068
acute renal failure due to ingestion of the gallbladder of grass carp: report of 3 cases with review of literature.gallbladder toxin of the grass carp (ctenopharyngodon idella) is a less well-known cause of acute renal failure. three cases are reported and the diagnosis is primarily clinical. all our patients presented initially with gastrointestinal upset including nausea, vomiting, diarrhea, and abdominal fullness. these symptoms usually occurred with 10 min to 18 h after the ingestion the raw gallbladder of the grass carp. all our cases developed acute renal failure and in two of them toxic hepatitises we ...19938290711
effect of detergent on the response to temperature and growth of grass carp, ctenopharyngodon idella. 19938387840
composite fish culture for mosquito control in rice fields in southern india.composite culture of edible fishes (common carp, cryprinus carpio; silver carp, hypopthalmithys molitrix, grass carp, ctenopharyngodon idella; catla, catla catla; rohu, labeo rohita; and mrigal, cirrhinus mrigala) in rice fields in the cauvery delta of tamil nadu, southern india, resulted in 81.0% reduction in the immature mosquito population of anophelines and 83.5% of culicines. analysis of fish feces for mosquito larval head capsules showed that common carp and silver carp are effective larvi ...19947777919
effect of nitrite on the survival of grass carp, ctenopharyngodon idella (val.), with relation to chloride. 19948130420
[clostridium bifermentans infection in grass carp (ctenopharyngodon idella)].the outbreak of an infection with clostridium bifermentans in grass carp (ctenopharyngodon idella) from a polyculture fish pond is documented.19957786278
comparisons of heavy metal concentration of grass carp (ctenopharyngodon idella cuv. et val.) in a shallow eutrophic lake and a fish pond (possible effects of food contamination).nickel, cu, zn, cd, pb and hg concentrations were determined in gills, liver, kidney, muscle and gut of grass carp (ctenopharyngodon idella cuv. et val.) collected from lake balaton (hungary) and from a fish pond of a fish farm nearby. the body weight normalised heavy metal concentrations in different organs/tissues were compared and ordered for both lake balaton and the fish farm. metal concentrations, in most cases were highest in kidney or in liver, and lowest in muscle or in gut. nickel conc ...19968867148
effects of sublethal doses of fenvalerate (a synthetic pyrethroid) administered continuously for four weeks on the blood, liver, and muscles of a freshwater fish, ctenopharyngodon idella. 19968672077
qualitative and quantitative strategies of thermal adaptation of grass carp (ctenopharyngodon idella) cytoplasmic malate dehydrogenases.malate dehydrogenase isozymes of grass carp,ctenopharyngodon idella, were identified by mitochondrial preparation and thermal denaturation. the structural and kinetic characteristics of chromatographically separated thermostable and thermolabile cmdhs were different in (1) half-life at 42°c, 10 min and 24 h, respectively, (2) optimal substrate, oxaloacetate and malate, concentrations, and (3) the apparent michaelis-menten constants of nadh and oxaloacetate.total mdh activity in white muscle of 1 ...199624193991
essential fatty acid requirements in carp.till date, many workers have demonstrated the nutritive value of dietary lipids and the qualitative and quantitative requirements for essential fatty acid (efa) in many cultivable fish. from the data accrued, a categorization can be made for the efa requirement as follows: type i (require n-6 fatty acids), type ii (require both n-6 and n-3 fatty acids) and type iii (require n-3 fatty acids). common carp cyprinus carpio and grass carp ctenopharyngodon idella are codified as type ii fish based on ...19968766970
epitheliocystis disease in cultured pacu (piaractus mesopotamicus) in brazil.epitheliocystis disease was diagnosed in a cultured pacu (piaractus mesopotamicus holmberg, 1893) stock on the basis of gross pathological (greyish-white, pinpoint- or pinhead-sized focal areas, connected strongly to the gill lamellae) and histopathological examinations (hypertrophic cells 10-40 microns in size, having a well-defined wall and a central inclusion or characteristic granular content stained with giemsa). the disease did not occur among fishes (cyprinus carpio l., ctenopharyngodon i ...199910497824
cryopreservation of spermatozoa in cyprinid fishes.the present study investigated semen cryopreservation in cyprinid fish using computer-assisted sperm motility analysis for viability control. spermatozoa of the bleak, chalcalbumus chalcoides, were used as a basic model to describe the toxic and cryoprotective effects of internal and external cryoprotectants, their most effective concentrations and combinations, the freezing and thawing conditions, and the effects of equilibration. we also used these data to develop a cryopreservation protocol f ...200011191871
polymorphism in ornamental and common carp strains (cyprinus carpio l.) as revealed by aflp analysis and a new set of microsatellite markers.forty-seven new microsatellite markers were generated and applied, together with the aflp (amplified fragment length polymorphism) technique using two different enzyme combinations, to the genetic analysis of two carp species, cyprinus carpio l. and ctenopharyngodon idella. the extent of polymorphism and the genetic relationships between nine carp populations were studied. the incidence of microsatellites containing ca and ct motifs was estimated to be one every 17.4 and one every 126.3 kb, resp ...200111713665
direct and trophic contamination of the herbivorous carp ctenopharyngodon idella by inorganic mercury and methylmercury.a comparative analysis of inorganic mercury (hg(ii)) and methylmercury (mmhg) bioaccumulation by the herbivorous fish ctenopharyngodon idella, via the direct and "direct+trophic" routes, was investigated experimentally. the direct contamination was based on fairly low exposure conditions (hg(ii), 300 ng small middle dotl(-1); mmhg, 30 ng small middle dotl(-1)), for 15 and 30 days. the trophic uptake was based on ingestion of macrophyte leaves (elodea densa), previously exposed for 87 days to sim ...200111534952
identification and ultrastructural characterization of a novel virus from fish.during routine investigations on fish, a virus (isolate df 24/00) with novel morphological features and hitherto undescribed morphogenesis was isolated from a white bream (blicca bjoerkna l.; teleostei, order cypriniformes). cell-free virions consist of a rod-shaped nucleocapsid (120-150x19-22 nm) similar to that seen in baculoviruses. the virion has a bacilliform shape (170-200x75-88 nm) reminiscent of rhabdoviruses with an envelope containing coronavirus-like spikes (20-25 nm). df 24/00 replic ...200111714959
molecular cloning of full-length cdna encoding delta-9 desaturase through pcr strategies and its genomic organization and expression in grass carp (ctenopharyngodon idella).desaturases are enzymes that catalyze double bond formation in fatty acids, which is a critical step in the synthesis of unsaturated fatty acids in organisms. desaturase cdna has been cloned from various species. here we report the cloning of a full-length cdna of delta(9)-desaturase from grass carp (ctenopharyngodon idella), using a combination of pcr techniques: reverse transcription-polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). the resolved cdna encompasses 2 ...200111170264
toxic and feeding deterrent effects of native aquatic macrophytes on exotic grass carp (ctenopharyngodon idella).declines of amphibians have been attributed to many factors including habitat degradation. the introduction of grass carp (ctenopharyngodon idella) as a biological agent for aquatic plant control in ponds and lakes managed narrowly for human recreation has likely contributed to amphibian declines through massive plant removal and associated habitat simplification and thus degradation. this research examined the interactions among grass carp and three midwestern aquatic plants (jussiaea repens, r ...200212211697
[studies of f1 of transgenic allotetraploid hybrids of carassius auratus red var. (female) x cyprinus carpio (male)].the tetraploid fish has been developed by assortative breeding the hybrids of carassius auratus red var. (female) x cyprinus carpio (male), which has the stable genetic characters and can reproduce themselves. an "all-fish" recombinant dna construct (pcagcghc) containing common carp beta-actin gene promoter and cdna for grass carp (ctenopharyngodon idella) growth hormone gene was introduced into fertilized eggs of the allotetroploid fish through microinjection as soon as artificial insemination ...200212043572
cdna nucleotide sequence coding for stearoyl-coa desaturase and its expression in the zebrafish (danio rerio) embryo.a cdna sequence of stearoyl-coa desaturase (scd) was determined from zebrafish (danio rerio) and compared to the corresponding genes in several teleosts. zebrafish scd cdna has a size of 1,061 bp, encodes a polypeptide of 325 amino acids, and shares 88, 85, 84, and 83% similarities with tilapia (oreochromis mossambicus), grass carp (ctenopharyngodon idella), common carp (cyprinus carpio), and milkfish (chanos chanos), respectively. this 1,061 bp sequence specifies a protein that, in common with ...200314579408
feeding ecology and food composition of the black carp mylopharyngodon piceus and the grass carp ctenopharyngodon idella inhabiting the fish pond of al-abbassa fish hatchery with emphasis given to vector snails.stomach contents of m. piceus and c. idella collected from earthen fish ponds at al-abbassa fish hatchery during different seasons were examined. food items were analyzed by three methods; the numerical (n%) percentage of point assessment (p%) and frequency of occurrence (f%). a comparison of the different methods emphasized the importance of snails as the major food resources in the diet of m. piceus during all seasons. during winter, stomachs of the most fish samples collected from fish ponds ...200415287186
molecular phylogeny of congeneric monogenean parasites (dactylogyrus): a case of intrahost speciation.dactylogyrus species (dactylogyridae: monogenea) are a group of monogenean gill parasites that are highly specific to freshwater fish of the family cyprinidae. dactylogyrus species were sampled from 19 cyprinids and one percid collected in europe. using partial 18s rdna and its1 sequences, a phylogeny of 51 dactylogyrus species was reconstructed to investigate the patterns of parasite speciation and diversification. three main dactylogyrus lineages were recognized from all phylogenetic trees, th ...200415212381
temperature effects along the reproductive axis during spawning induction of grass carp (ctenopharyngodon idella).for grass carp (ctenopharyngodon idella) raised in the ivory coast (with water temperatures of 26-31 degrees c), induced spawning is obligatory for fry production. however, ovulation rates following hormonal treatment are often low. we hypothesized that high temperatures are an inhibiting factor for the reproductive axis (brain-pituitary-gonad) in these conditions. by in vivo and in vitro experiments, we tried to determine the thermosensitive steps during spawning induction. we compared gonadotr ...200415028520
antibiotic resistance pattern of motile aeromonads from farm raised fresh water fish.motile aeromonads isolated from the intestines of farm-raised freshwater fish such as catla catla, labeo rohita and ctenopharyngodon idella have been characterized to species level. morphological and physiological grouping revealed 61% aeromonas hydrophila, 30% aeromonas caviae, 7% aeromonas sobria and 2% which remained unidentified. hemolytic activity was detected mostly in a. hydrophila, while only half of the a. sobria and a. caviae showed this activity. antibiotic resistance patterns of the ...200515747443
stearoyl-coa desaturase expression and fatty acid composition in milkfish (chanos chanos) and grass carp (ctenopharyngodon idella) during cold acclimation.desaturation of fatty acids is an important adaptation mechanism for fish to maintain membrane fluidity under thermal stress. to comprehend the temperature adaptation mechanism in fish, we investigated the difference in the changes of stearoyl-coa desaturase expression and fatty acid composition between milkfish and grass carp under cold acclimation. we find that in both fish the proportions of unsaturated fatty acids at 15 degrees c are all higher than those at 25 degrees c. in milkfish delta(9 ...200515820139
identification of immune genes in grass carp ctenopharyngodon idella in response to infection of the parasitic copepod sinergasilus major.the parasitic copepod sinergasilus major is an important pathogen of grass carp ctenopharyngodon idella. to understand the immune response of grass carp to the copepod infection, suppression subtractive hybridization method was employed to characterize genes up-regulation during the copepod infection in liver and gills of the fish. one hundred and twenty-two dot blot positive clones from infected subtracted library were sequenced. searching available databases by using these nucleotide sequences ...200515868187
sox genes in grass carp (ctenopharyngodon idella) with their implications for genome duplication and evolution.the sox gene family is found in a broad range of animal taxa and encodes important gene regulatory proteins involved in a variety of developmental processes. we have obtained clones representing the hmg boxes of twelve sox genes from grass carp (ctenopharyngodon idella), one of the four major domestic carps in china. the cloned sox genes belong to group b1, b2 and c. our analyses show that whereas the human genome contains a single copy of sox4, sox11 and sox14, each of these genes has two co-or ...200617129566
piscicidal plants of nepal: preliminary toxicity screening using grass carp (ctenopharyngodon idella) fingerlings.a survey of traditional fishermen in chitwan district of nepal identified the native names of 97 species of plants believed to possess piscicidal properties. ninety-two were collected and identified. eighty-two of these were tested for toxicity using grass carp (ctenopharyngodon idella) fingerlings (2-3 cm total length) as test organisms. thirty-six species that killed the fingerlings within 2 h at 1% (w/v) extract concentration or less were categorized as poisonous. forty-six species did not ki ...20063695553
biochemical hepatic alterations and body lipid composition in the herbivorous grass carp (ctenopharyngodon idella) fed high-fat diets.high-fat diets may have favourable effects on growth of some carnivorous fish because of the protein-sparing effect of lipids, but high-fat diets also exert some negative impacts on flesh quality. the goal of the study was therefore to determine the effects of fat-enriched diets in juvenile grass carp (ctenopharyngodon idella) as a typical herbivorous fish on growth and possible lipid metabolism alterations. three isonitrogenous diets containing 2, 6 or 10 % of a mixture of lard, maize oil and f ...200616611380
chemical defenses promote persistence of the aquatic plant micranthemum umbrosum.five of the most common macrophytes from an aquaculture facility with high densities of the herbivorous asian grass carp (ctenopharyngodon idella) were commonly unpalatable to three generalist consumers-grass carp and the native north american crayfishes procambarus spiculifer and p. acutus. the rooted vascular plant micranthemum umbrosum comprised 89% of the total aboveground plant biomass and was unpalatable to all three consumers as fresh tissues, as homogenized pellets, and as crude extracts ...200616586032
characterization and expression analysis of tnf-related apoptosis inducing ligand (trail) in grass carp ctenopharyngodon idella.trail (apo2 ligand) described as a type ii transmembrane protein belonging to the tnf superfamily can induce apoptotic cell death in a variety of cell types. in the present study, a putative cdna sequence encoding the 299 amino acids of trail (gc-trail) and its genomic organization were identified in grass carp ctenopharyngodon idella. the predicted gc-trail sequence showed 44 and 41% identities to chicken and human trails, respectively. in a domain search, a tumor necrosis factor homology domai ...200616216337
molecular cloning and sequencing of hemoglobin-beta gene of channel catfish, ictalurus punctatus rafinesque.the hemoglobin-beta gene of channel catfish, ictalurus punctatus, was cloned and sequenced. total rna from head kidneys was isolated, reverse transcribed and amplified. the sequence of the channel catfish hemoglobin-beta gene consists of 600 nucleotides. analysis of the nucleotide sequence reveals one open reading frame and 5'- as well as 3'-untranslated regions. the open reading frame of the sequence potentially encodes 148 amino acids with a calculated molecular mass of 16.3 kda. the pi and ch ...200620035483
isozymes in androgenetic and gynogenetic white amur, gynogenetic carp, and carp-amur hybrids.gynogentic and androgenetic progeny appeared in crosses between white amur, ctenopharyngodon idella, and carp, cyprinus carpio. hemoglobin, plasma general proteins, and plasma isoenzymes were studied by electrophoresis to determine inheritance patterns. electrophoretic bands indicated that gynogenetic white amur had no paternal inheritance from carp and that adrogenetic white amur also were pure white amur. gynogeneiss in carp was confirmed by the absence of paternal inheritance. hemoglobins, ge ...2006939912
occurrence of camallanus cotti in greatly diverse fish species from danjiangkou reservoir in central china.two thousand four hundred fifty-eight fish comprised of 53 species were captured in the danjiangkou reservoir, in the northwestern part of hubei province, central china during 2004, to examine camallanus cotti infections. we found that 19 cypriniform, 3 siluriforme, and 4 perciforme fishes were infected by the nematode. our study revealed the species, hemiculter bleekeri bleekeri, culter oxycephaloide, pseudolaubuca sinensis, acanthobrama simony, mylopharyngodon piceus, ctenopharyngodon idella, ...200717351790
molecular cloning, promoter analysis and induced expression of the complement component c9 gene in the grass carp ctenopharyngodon idella.complement-mediated killing of pathogens through lytic pathway is an important effector mechanism of innate immune response. c9 is the ninth member of complement components, creating the membrane attack complex (mac). in the present study, a putative cdna sequence encoding the 650 amino acids of c9 and its genomic organization were identified in grass carp ctenopharyngodon idella. the deduced amino acid sequence of grass carp c9 (gcc9) showed 48% and 38.5% identity to japanese flounder and human ...200717604124
the first non-mammalian cxcr3 in a teleost fish: gene and expression in blood cells and central nervous system in the grass carp (ctenopharyngodon idella).a novel fish chemokine receptor gene, chemokine (c-x-c motif) receptor 3 (cxcr3)-like was isolated from the grass carp ctenopharyngodon idella, with its full-length genomic sequence. the cdna of grass carp cxcr3-like (gccxcr3-like) consists of 1261 bp with a 49 bp 5'-utr and a 189bp 3'-utr. an open reading frame of 1023 bp encodes a 341-amino acid peptide, with seven transmembrane helices. the deduced amino acid sequence showed the same sequence identities (37.8%) with its counterparts in goat a ...200716930712
intelectin gene from the grass carp ctenopharyngodon idella: cdna cloning, tissue expression, and immunohistochemical localization.the cdna encoding grass carp intelectin was isolated from a head kidney cdna library, and termed gcintl. the deduced amino acid sequence of gcintl consists of 318 amino acids, and about 55% identical and 74% similar to human intelectin, which is a new type of lectin recognizing galactofuranose, and plays a role in the recognition of bacteria-specific components in animal hosts. the gcintl gene consists of seven exons and six introns, spacing over approximately 3 kb of genomic sequence. phylogene ...200717101278
natural origins of off-flavours in fish related to feeding habits.the objective of our study was to survey the accumulated 2-methylisoborneol (mib) and geosmin (gsm) in fillets of five important farmed fish species in hungarian aquaculture in relation to mib and gsm concentrations in water and sediment in the aquatic systems where they were raised: (the planktivorous silver carp (hypophthalmichthys molitrix), the herbivorous grass carp (ctenopharyngodon idella), the bottom-feeding omnivorous common carp (cyprinus carpio), the omnivorous tilapia (oreochromis ni ...200717489423
concentrations of pentachlorophenol (pcp) in fish and shrimp in jiangsu province, china.pentachlorophenol (pcp) concentrations were determined in 55 samples of fish, shrimp, crabs, frogs and turtles collected in jiangsu province and 55 freshwater shrimp samples collected from the huiming fish market, nanjing, china between 2003 and 2004. pcp concentrations ranged from less than the method detection limit (mdl: 0.5 microg/kg ww) to 61 microg/kg ww, with a mean of 5.2 microg/kg ww in 55 samples collected across jiangsu province and a mean of 0.5 microg/kg ww for freshwater shrimp col ...200717537482
light and scanning electron microscopic study of balantidium ctenopharyngodoni chen, 1955 (class: litostomatea) from china.redescription of balantidium ctenopharyngodoni "chen (acta hydrobiol sin 1:123-164, 1955)", collected from the hindgut of grass carp (ctenopharyngodon idella), especially the segment of 6-10 cm upstream from the anus, from honghu lake, hubei province, central china in november 2005, is presented in this paper to complete chen's description at both light and scanning electron microscopic levels. some revisions were done: the vestibulum is fairly symmetrical, with compactly arranged cilia rather t ...200717265089
molecular identification and expression analysis of tumor necrosis factor receptor-associated factor 2 in grass carp ctenopharyngodon idella.tumor necrosis factor receptor-associated factor 2 (traf2) is a crucial component of almost the entire tumor necrosis factor receptor superfamily signaling pathway. in the present study, a traf2 gene has been cloned from grass carp (ctenopharyngodon idella) by reverse transcription-polymerase chain reaction and rapid amplification of cdna ends. the full-length cdna is 3162 bp, including a 60 bp 5' untranslated region (utr), a 1611 bp open reading frame, and a 1491 bp 3' utr. the polyadenylation ...200717989877
hypolipidaemic effects of fenofibrate and fasting in the herbivorous grass carp ( ctenopharyngodon idella) fed a high-fat diet.we investigated whether the hypolipidaemic effect of fenofibrate and fasting observed in most omnivorous mammals may also apply to herbivorous fish. grass carp (ctenopharyngodon idella) fed a high-fat (8 %) diet exhibited a marked increase in blood lipids and body fat after 6 weeks. they were then treated with fenofibrate (100 mg/kg body weight) in the same high-fat diet for 2 weeks, followed by fasting for 1 week. plasma lipid concentration, body fat amount, fatty acid composition, plasma thiob ...200818445306
enzyme-linked immunosorbent assay of changes in serum levels of growth hormone (cgh) in common carps (cyprinus carpio).the aim of the present study was to purify the common native carp growth hormone (ncgh), produce monoclonal antibodies (mabs) to common native carp growth hormone (ncgh), and further enhance the sensitivity of enzyme-linked immunosorbent assays (elisa) for ncgh. additionally, we investigated changes in serum ncgh levels in carps raised in different environmental conditions. the recombinant grass carp (ctenopharyngodon idella) growth hormone was purified and used as antigen to immunize the rabbit ...200818239894
study of apiosoma piscicola (blanchard 1885) occurring on fry of freshwater fishes in hongze, china with consideration of the genus apiosoma.apiosoma piscicola (blanchard 1885) was reported from fry of carassius auratus (var. pengze) and ctenopharyngodon idella during parasite surveys in may 2005 and june 2006 at hongze lake fish hatchery, jiangsu province, china. twelve morphometric parameters were used to describe this peritrich in detail in the present study and comparisons were made with other reports. its synonyms, glossatella cylindriformis (chen 1955) and apiosoma magna (banina 1968), were clarified based on reliable data. a. ...200818247054
characterization and expression analysis of tnfr-associated factor 1 (traf1) in grass carp ctenopharyngodon idella.tnf receptor associated factor 1 (traf1) plays an important role in regulating the tnf signaling and protecting cells from apoptosis. in the present study, a traf1 gene has been cloned from grass carp (ctenopharyngodon idella) by reverse transcription (rt)-pcr and rapid amplification of cdna ends (race). the full-length cdna is 2235bp, including a 250bp 5' utr (untranslated region), a 1659bp open reading frame, and a 326bp 3' utr. the polyadenylation signal (aataaa, aataa) and one mrna instabili ...200817868904
detection of cutaneous antibodies in excised skin explants from grass carp, ctenopharyngodon idella (valenciennes), immune to scophthalmus maximus rhabdovirus.this study determined whether cutaneous antibodies were present in excised skin explants of grass carp, ctenopharyngodon idella, immune to scophthalmus maximus rhabdovirus (smrv). culture fluid from immune skin explants were assayed by indirect enzyme-linked immunosorbent assay (ielisa), western blot, indirect immunofluorescent assay (ifa) and flow cytometry (fcm). ielisa showed that cutaneous antibody titres were much lower (1:12) than antiserum titres (1:1458) from intraperitoneally immunized ...200818482386
complete mitochondrial genome of the grass carp (ctenopharyngodon idella, teleostei): insight into its phylogenic position within cyprinidae.genome comparison has shed light on many fields of both basic and applied research, including the study of species phylogeny. grass carp (ctenopharyngodon idella) belongs to cyprinidae, the largest freshwater fish family; but which subfamily it belongs to remains a controversial issue. in this study, the complete mitochondrial genome (mitogenome) sequence of grass carp was determined and phylogenetic analyses of all mitochondrial protein-coding genes and a nuclear gene (rag 2) were conducted to ...200818706492
cdna cloning and characterization of temperature-acclimation-associated light meromyosins from grass carp fast skeletal muscle.the three types of cdna clones, previously defined as the 10 degrees c, intermediate and 30 degrees c-types [tao, y., kobayashi, m., liang, c.s., okamoto, t., watabe, s., 2004. temperature-dependent expression patterns of grass carp fast skeletal myosin heavy chain genes. comp. biochem. physiol. b 139, 649-656], were determined for their 5'-regions which encoded at least the c-terminal half of myosin rod, light meromyosin (lmm), in fast skeletal muscles of grass carp ctenopharyngodon idella. the ...200818055241
cloning, characterization and expression analysis of simp (source of immunodominant mhc-associated peptides) in grass carp ctenopharyngodon idella.simp (source of immunodominant mhc-associated peptides) plays a key role in n-linked glycosylation with the active site of oligosaccharyltransferase, being the source of mhc-peptides in the mhc i presentation pathway. in the present study, the simp gene has been cloned from grass carp ctenopharyngodon idella by rapid amplification of cdna ends (race). the full length of the cdna sequence is 4384bp, including a 1117bp 5' utr (untranslated region), a 2418bp open reading frame, and a 849bp 3' utr. ...200818407520
acute toxicity of nuvan, an organophosphate to freshwater fish ctenopharyngodon idella and its effect on oxygen consumption.the acute toxicity of nuvan to the grass carp ctenopharyngodon idella was determined using static and continuous flow through system for 24, 48, 72 and 96 hr. the median lethal concentration (lc50) values were 13.1, 10.9, 9.8 and 6.5 mg l(-1) respectively in static system and 10.7, 9.5, 8.0 and 7.5 mg l(-1) respectively in continuous flow through system. a reduction in oxygen consumption is observed when the fish is exposed to the toxicant and the mortality is due to effect of metabolism of ener ...200920329401
genomic organization and expression analysis of toll-like receptor 3 in grass carp (ctenopharyngodon idella).toll-like receptor 3 (tlr3) participates in the innate immune response by recognizing viral pathogens. to investigate grass carp immune system responding to gcrv (grass carp reovirus) infection, the full-length cdna sequence and genomic organization of grass carp tlr3 (citlr3) was identified and characterized. the full-length genome sequence of citlr3 is composed of 5668 nucleotides, including five exons and four introns. the full-length of citlr3 cdna is 3681 bp in length and encodes a polypept ...200919545638
a novel male-specific dna sequence in the common carp, cyprinus carpio.in this study, we identified a sex-specific dna marker using randomly amplified polymorphic dna (rapd) fingerprinting in yellow river carps (cyprinus carpio from the yellow river). two hundred and twenty random primers were used in pooled dna samples and individual dna samples from male and female fish for rapd fingerprinting. when using the primer s2107, a novel sex-specific pcr product was identified in all male individuals. dna sequencing revealed that this 909 bp long dna fragment has a low ...200919401231
glutathione s-transferase as a biomarker in the antarctic bivalve laternula elliptica after exposure to the polychlorinated biphenyl mixture aroclor 1254.glutathione s-transferases (gsts) are a family of multifunctional enzymes involved in cellular detoxification that catalyze the attachment of electrophilic substrates to glutathione. two classes of gsts related to the rho and sigma classes of enzymes in antarctic bivalves have been cloned from laternula elliptica. the full-length cdna of rho class gst (legstr) is 1530bp in length and contains an open reading frame (orf) of 672bp encoding 223 amino acid residues. the deduced amino acid sequences ...200919651242
a fine structural review on the spermatozoa of cyprinidae with attention to their phylogenetic implications.the fine structural organization and dimensions of spermatozoa from species of 4 subfamilies of the cyprinidae (barbus barbus, carassius carassius, cyprinus carpio carpio, cyprinus carpio haematopterus, abramis brama, alburnoides bipunctatus, alburnus alburnus, chalcalburnus chalcoides mento, chondrostoma nasus, hypophthalmichthys molitrix, leuciscus cephalus, phoxinus phoxinus, rutilus rutilus, rutilus meidingerii, scardinius erythrophthalmus, vimba vimba and ctenopharyngodon idella) are compar ...200919688692
myosin heavy chain genes expressed in juvenile and adult silver carp hypopthalmichthys molitrix: novel fast-type myosin heavy chain genes of silver carp.silver carp hypopthalmichthys molitrix is eurythermal temperate fish, whose muscle is considered to express several types of myosin heavy chain (myh) genes at different stages of its growth and to adjust to the environmental temperature. in this study, myh genes expressed in the muscles of juvenile and adult silver carp were investigated. five types of myh cdna clone were isolated from silver carp (h. molitrix) by race strategy using a set of fast-type myh specific primers, and termed scmyh(f1), ...200919100315
twenty-five polymorphic microsatellite markers from grass carp (ctenopharyngodon idella). 200919531113
identification and expression profiling analysis of grass carp ctenopharyngodon idella lgp2 cdna.lgp2 (laboratory of genetics and physiology 2), a homologue of rig-i (retinoic acid inducible gene-i) and mda5 (melanoma differentiation associated gene 5) without the card (caspase activation and recruitment domain) required for signaling, plays a pivotal role in modulating signaling by rig-i and mda5 for interferon (ifn) synthesis. in this study, a novel lgp2 gene from grass carp ctenopharyngodon idella (designated as cilgp2) was isolated and characterized. the full-length cdna of cilgp2 was o ...201020420913
the first non-mammalian cxcr5 in a teleost fish: molecular cloning and expression analysis in grass carp (ctenopharyngodon idella).chemokines, a group of small and structurally related proteins, mediate chemotaxis of various cell types via chemokine receptors. in mammals, seven different cxc chemokine receptors denoted as cxcr1 to cxcr7 have been reported. however, the chemokine receptor cxcr5 has not been reported in other vertebrates.201020504365
simple gas chromatographic method for the quantification of total cholesterol in fish meats.six fish species-cirrhinus mrigala (morakhi), labeo rohita (danbhro), catla catla (thalli), wallago attu (jarko), ctenopharyngodon idella (grass), and cyprimus carpio (gulfam)--commonly found in the pakistani indus river, were selected for fat and cholesterol evaluation. fat content was quantified by the folch method and cholesterol content was determined by a simple gc method. the application of the proposed method to quantify cholesterol content revealed variations among the six breeds evaluat ...201020922959
molecular cloning, characterization and expression analysis of interferon-β promoter stimulator 1 (ips-1) gene from grass carp ctenopharyngodon idella.ips-1 (interferon-β promoter stimulator 1), also known as mavs/visa/cardif, plays a central role in antiviral immunity. in this manuscript, we cloned and characterized ips-1 from grass carp ctenopharyngodon idella (designated as ciips-1). the ciips-1 cdna is 2412 bp long and consists of a 5' untranslated region (utr) of 124 bp, a 3' utr of 497 bp with three cytokine rna instability motifs (attta) and a polyadenylation signal (aataaa), and an open reading frame (orf) of 1791 bp encoding a polypep ...201021078397
ig heavy chain genes and their locus in grass carp ctenopharyngodon idella.the cdna and genomic sequences of igd and igz were characterized in grass carp ctenopharyngodon idella in the present study, and with the identification of a bac clone covering zeta, mu, and delta genes, the igh locus containing these ig genes and other v, d, j genes was also illustrated in this fish. secretory and membrane-bound igz were identified, with two transmembrane exons spliced within the ch4 exon, as reported in igm of mammals and igz in other teleost fish. the first and second constan ...201020558301
identification of immune-relevant genes by expressed sequence tag analysis of head kidney from grass carp (ctenopharyngodon idella).grass carp is the third largest aquaculture species in global production. however, genomic research of this species has been limited. to identify immune-related genes in grass carp, a normalized full-length cdna library was constructed from head kidney tissues, and 6432 randomly selected clones were sequenced. 5289 high quality expressed sequence tags (est) were generated and assembled into 2687 unigenes. among them, 1585 unigenes showed significant similarity with known sequences in public data ...201020403773
molecular characterization and expression analysis of nuclear oligomerization domain proteins nod1 and nod2 in grass carp ctenopharyngodon idella.nuclear oligomerization domains (nods) are cytosolic pattern recognition receptors (prrs) to detect bacterial component. in this study, the molecular cloning and genomic characterization of grass carp nod1 (gcnod1) and grass carp nod2 (gcnod2) were reported. the complete open reading frame of gcnod1 contains 2814 bp, encoding a 937-amino acid polypeptide. the gcnod2 cdna sequence encodes 982-amino acid polypeptide. both gcnod1 and gcnod2 possess three conserved domains: carboxy terminal leucine ...201019766192
bioaccumulation of heavy metals in fish tissues of a freshwater lake of bhopal.contamination of heavy metals, namely, lead, cadmium, zinc, nickel, copper, chromium and mercury was evaluated in the samples of water and tissues of labeo rohita and ctenopharyngodon idella of upper lake of bhopal collected during summer, rainy and winter seasons of 2005-2006. different organs of the fishes accumulated varying quantities of different heavy metals. in l. rohita, accumulation of heavy metals was in the sequence liver>kidney>gills>muscles, and in c. idella, it was gills>liver>kidn ...201019123040
[cloning and functional analysis of the collectin gene from the grass carp ctenopharyngodon idella].the grass carp (ctenopharyngodon idella) collectin gene was cloned from mixed liver and kidney cdna library. the full length sequence of grass carp collectin was 1128 bp, contained a 5' untranslated region of 229 bp and a 3' untranslated region of 104 bp. the open reading frame of grass carp collectin was 795 bp which could code a 264 amino acids polypeptide, including a terminal codon. phylogenetic analyses showed that grass carp collectin shared the highest homology with that of zebrafish (dan ...201020353088
effect of sanguinarine from the leaves of macleaya cordata against ichthyophthirius multifiliis in grass carp (ctenopharyngodon idella).the ciliate ichthyophthirius multifiliis is one of the most pathogenic parasites of fish maintained in captivity. in this study, effects of crude extracts, fractions, and compounds from the leaves of macleaya cordata against i. multifiliis were investigated under in vitro conditions by bioactivity-guided isolation method. the dried ethanol extract of m. cordata was extracted successively in a separating funnel with petroleum ether, ethyl acetate, chloroform and n-butanol. among them, only the ch ...201020625767
molecular identification and expression analysis of toll-like receptor 3 in common carp cyprinus carpio.the toll-like receptor (tlr) family is an evolutionarily conserved component of the innate immune system that responds to specific pathogen-associated molecular patterns (pamp) during infections. tlr3 is a putative pattern recognition receptor (prr) for dsrna, a common by-product of viral replication. this study reports the identification and characterization of a full-length orthologue of tlr3 (cctlr3) in the common carp cyprinus carpio. it is 2899 base pairs (bp) encoding 904 amino acid residu ...201020557647
comparative susceptibility of carp fingerlings to lernaea cyprinacea infection.study was conducted to find out the comparative susceptibility of fingerlings of seven species of carps (labeo fimbriatus, l. rohita, l. calbasu, catla catla, ctenopharyngodon idella, cyprinus carpio and hypophthamichthys molitrix) grown under both mono and polyculture to lernaea cyprinacea infection. under monoculture, c. carpio, l. rohita and l. calbasu, did not acquire lernaea infection and were thus considered resistant, whereas c. idella, h. molitrix, c. catla and l. fimbriatus were suscept ...201021255932
molecular cloning, characterization and expression of fsh and lh beta subunits from grass carp (ctenopharyngodon idella).follicle-stimulating hormone beta subunit (fshbeta) and luteinizing hormone beta subunit (lhbeta) have been cloned and characterized from the pituitary of grass carp (ctenopharyngodon idella). the full length of fshbeta and lhbeta cdna was 393 bp and 441 bp, with open reading frame encoding proteins of 130 and 146 amino acids, respectively. phylogenetic analysis of the proteins of fshbeta and lhbeta showed a high homology with other fishes. homology analysis also indicated that lhbeta has higher ...201018777101
screening and identification of male-specific dna fragments in common carps cyprinus carpio using suppression subtractive hybridization.in this study, a sex subtractive genomic dna library was constructed using suppression subtractive hybridization (ssh) between male and female cyprinus carpio. twenty-two clones with distinguishable hybridization signals were selected and sequenced. the specific primers were designed based on the sequence data. those primers were then used to amplify the sex-specific fragments from the genomic dna of male and female carp. the amplified fragments from two clones showed specificity to males but no ...201020646164
identification of a vasa homologue gene in grass carp and its expression pattern in tissues and during embryogenesis.the rna helicase vasa is a member of the dead box protein family that plays an indispensable role in germ cell determination in eukaryotes such as in drosophila and xenopus species. in this study, the grass carp homologue of the drosophila vasa gene, civasa (ctenopharyngodon idella vasa) was obtained using degenerate primers in rt-pcr and 5' and 3' rapid amplification of cdna end polymerase chain reaction from the grass carp ovary smart cdna. this cdna sequence encodes a 670 amino acid residue p ...201020471489
molecular characterization and expression analysis of the ifn-gamma related gene (ifn-gammarel) in grass carp ctenopharyngodon idella.interferon gamma (ifn-gamma), the only member of the type ii class of interferons, has been identified in teleost fish. in addition to the ifn-gamma gene, fish possess an ifn-gamma related gene (ifn-gammarel) neighbouring the authentic ifn-gamma gene in the genome. in the present study, the cdna sequence encoding 167 amino acids of ifn-gammarel and its genomic organization were identified in grass carp ctenopharyngodon idella. the predicted protein sequence of grass carp ifn-gammarel (gcifn-gamm ...201019800136
the gene and virus-induced expression of irf-5 in grass carp ctenopharyngodon idella.the interferon regulatory factor 5 (irf-5) is known to be involved in the innate immune response and in the regulation of dna damage-induced apoptosis. in the present study, the cdna and genomic sequences of irf-5 were identified in grass carp (ctenopharyngodon idella). the cdna of grass carp irf-5 (gcirf-5) contains an open reading frame (orf) of 1560 nucleotides, encoding a putative 519 amino acid protein, which showed 34.5-83.9% identity to irf-5 homologues from mammals, amphibian, avian and ...201019896215
molecular cloning and immune responsive expression of mda5 gene, a pivotal member of the rlr gene family from grass carp ctenopharyngodon idella.the cytoplasmic helicase protein mda5 (melanoma-differentiation-associated gene-5) recognizes long molecules of viral double-stranded rna (dsrna) and single-stranded rna with 5' triphosphate and mediates type i interferon secretion. in the present study, the first mda5 gene in fish was cloned and characterized from grass carp ctenopharyngodon idella. the full length of the c. idella mda5 (cimda5) cdna is 3233 nucleotides in length and encodes a polypeptide of 961 amino acids. the deduced amino a ...201020109556
the polymorphism and haplotype of tlr3 gene in grass carp (ctenopharyngodon idella) and their associations with susceptibility/resistance to grass carp reovirus.toll-like receptors (tlrs) have emerged as crucial sensors of invading microbes through recognition of pathogen-associated molecular patterns (pamps) in viruses, bacteria, fungi and protozoa. the polymorphisms in tlrs are closely associated with the resistance to pathogen infections. tlr3 involved in the recognition of double stranded rna in humans, mice, pigs and fishes. in present study, the nucleotide sequence polymorphisms of tlr3 gene in grass carp (ctenopharyngodon idella) (citlr3) were in ...201020869445
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