Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| biodiversity data mining from argus-eyed citizens: the first illegal introduction record of lepomis macrochirus macrochirus rafinesque, 1819 in japan based on twitter information. | an apparent illegal introduction of lepomis macrochirus macrochirus from yokohama city, kanagawa prefecture, japan, is reported based on a juvenile specimen and a photograph of two adults collected on 14 june 2015 and deposited in the kangawa prefectural museum of natural history. the specimens and photographs were initially reported on the internet-based social networking site, twitter. two specimens of carassius auratus, including an aquarium form, were also reported at the same locality and d ... | 2016 | 27110154 |
| autotriploid origin of carassius auratus as revealed by chromosomal locus analysis. | in the dongting water system, the carassius auratus (crucian carp) complex is characterized by the coexistence of diploid forms (2n=100, 2ncc) and polyploid forms. chromosomal and karyotypic analyses have suggested that the polyploid c. auratus has a triploid (3n=150, 3ncc) and a tetraploid origin (4n=200), respectively. however, there is a lack of direct genetic evidence to support this conclusion. in this paper, analysis of the 5s rdna chromosomal locus revealed that the 3ncc is of triploid or ... | 2016 | 27084707 |
| variation in susceptibility pattern of fish to argulus siamensis: do immune responses of host play a role? | branchiuran ectoparasites of the genus argulus can have extensive damaging effects on cultured fish. there exist no systematic studies that evaluate susceptibility or resistance of various carp species to argulus sp. and the underlying mechanisms. the present study aimed at identifying the most susceptible and resistant cultured species, studying settlement and survival of parasite on these species, and finally unravelling the variations of immune response in both resistant and susceptible speci ... | 2016 | 27084477 |
| development of vaccines against nocardiosis in fishes. | nocardiosis, one of the most systemic and devastating diseases, is currently emerging as an important disease of cultured marine and freshwater fishes. the causative agent of this disease is nocardia seriolae, a gram-positive acid-fast bacterium. an effective vaccine/vaccination strategy against this pathogen is necessary to control the significant loss in aquaculture practices. in this chapter, we present the vaccination/immunization protocol in fish against both live (sublethal) and inactivate ... | 2016 | 27076299 |
| brain mapping of ghrelin o-acyltransferase in goldfish (carassius auratus): novel roles for the ghrelinergic system in fish? | ghrelin o-acyltransferase (goat) is the enzyme responsible for acylation of ghrelin, a gut-brain hormone with important roles in many physiological functions in vertebrates. many aspects of goat remain to be elucidated, especially in fish, and particularly its anatomical distribution within the different brain areas has never been reported to date. the present study aimed to characterize the brain mapping of goat using rt-qpcr and immunohistochemistry in a teleost, the goldfish (carassius auratu ... | 2016 | 27064922 |
| periprandial changes and effects of short- and long-term fasting on ghrelin, goat, and ghrelin receptors in goldfish (carassius auratus). | the periprandial profile and effects of short- (7 days) and long-term (30 days) fasting on the ghrelinergic system were studied in goldfish (carassius auratus). plasma levels of acyl-ghrelin, desacyl-ghrelin, and ghrelin o-acyl transferase (goat) were analyzed by enzymoimmunoassays, and expression of preproghrelin, goat and growth hormone secretagogue receptors (ghs-r) was quantified by real-time pcr. circulating levels of acyl-ghrelin and goat rise preprandially, supporting the role of acyl-ghr ... | 2016 | 27062032 |
| experimental infection of aphanomyces invadans and susceptibility in seven species of tropical fish. | epizootic ulcerative syndrome (eus) causes by aquatic oomycete fungus, aphanomyces invadans is a dangerous fish disease of a wide range of fresh and brackish water, wild and farmed fish throughout the world. the objective of the present study was to determine the susceptibility of a number of tropical fish species to the eus and compare the severity of infection between experimental groups. | 2015 | 27047195 |
| multibiomarker toxicity characterization of uranium mine drainages to the fish carassius auratus. | the release of acidic effluents, naturally enriched in metals and radionuclides, is the main legacy of uranium mines. generally, metals dissolved by these acidic effluents can cause significant alterations in exposed organisms, with distinct toxicological outcomes. in this study, 72 individuals of the freshwater fish species carassius auratus were exposed in situ for different periods (8, 16, 24, and 48 h) to water from a pond (treatment pond (tp)) with a chemically treated effluent and a refere ... | 2016 | 27023822 |
| sialoglycoprotein isolated from eggs of carassius auratus ameliorates osteoporosis: an effect associated with regulation of the wnt/β-catenin pathway in rodents. | in the current study, ovariectomized (ovx) rats and the senescence-accelerated mouse strain p6 (samp6) were employed to establish models of postmenopausal osteoporosis and senile osteoporosis, respectively. the effects of treatment with sialoglycoprotein isolated from the eggs of carassius auratus (ca-sgp) on these two types of osteoporosis were investigated in vivo. results showed that ca-sgp significantly increased bone mineral density, ameliorated trabecular bone microstructure, and improved ... | 2016 | 27023001 |
| tactile reception in the outer head cover of goldfish (carassius auratus gibelio). | the first data on the responses of tactile receptors of the tonic, phasic, and mixed types to mechanic stimulation of the surface of head skin of fish obtained by means of noninvasive recording of potentials are presented. the sensitivity of skin tactile receptors is the highest in the circumoral and gular zones, which reflects their functional role in foraging behavior. | 2016 | 27021364 |
| effects of melatonin and green-wavelength led light on the physiological stress and immunity of goldfish, carassius auratus, exposed to high water temperature. | this study investigated the effects of increasing water temperature (22-30 °c) on the physiological stress response and immunity of goldfish, carassius auratus, and the ability of green light-emitting diode (led) irradiation or melatonin injections to mitigate this temperature-induced stress. to evaluate the effects of either green-wavelength led light or melatonin on stress in goldfish, we measured plasma triiodothyronine (t3), thyroxine (t4), and thyroid hormone receptor (tr) mrna expression; ... | 2016 | 27012684 |
| molecular characterization and expressing analysis of the c-type and g-type lysozymes in qihe crucian carp carassius auratus. | lysozyme as an important nonspecific immune factor, can kill bacteria by hydrolyzing β-1,4-glycosidic linkages of peptidoglycan layer, and plays an important role in innate immune response against pathogen infection. in the present study, we report molecular cloning, tissue distribution and functional characterization of the c-type and g-type lysozymes in qihe crucian carp carassius auratus (designated as ca-clys and ca-glys, respectively). the full-length of ca-clys and ca-glys cdna were cloned ... | 2016 | 27012394 |
| [evaluation of ivermectin's reproductive toxicity to male carassius auratus]. | as a new type of antiparasitic drugs, ivermectin (ivm) has been widely applied in agriculture, stock raising and aquaculture in china because of its broad spectrum and high efficiency. in order to evaluate the ivm' s reproductive toxicity to male crucian carp (carassius auratus), ivm was orally given to the experimental fish with different dosages and the gonadosomatic index (gsi), sexual hormone contents (including testosterone and estradiol) in serum and testis, γ-aminobutyric acid content in ... | 2015 | 26995928 |
| identification, cdna cloning, and characterization of luteinizing hormone beta subunit (lhb) gene in catla catla. | reproductive hormones play a significant role in the gonadal development and gametogenesis process of animals. in the present study luteinizing hormone beta, (lhb) subunit gene was cloned and characterized from the brain of catla catla. the lhb full-length of cdna sequence is 629 bp which consists of 43bp 5'-utr (untranslated region) 447bp, orf(open reading frame) and 139 bp of 3'-utr respectively. the coding region of lhb gene encoded a peptide of 148 amino acids. the coding sequence of lhb gen ... | 2016 | 26980432 |
| tributyltin disrupts feeding and energy metabolism in the goldfish (carassius auratus). | tributyltin (tbt) can induce obesogen response. however, little is known about the adverse effects of tbt on food intake and energy metabolism. the present study was designed to investigate the effects of tbt, at environmental concentrations of 2.44 and 24.4 ng/l (1 and 10 ng/l as sn), on feeding and energy metabolism in goldfish (carassius auratus). after exposure for 54 d, tbt increased the weight gain and food intake in fish. the patterns of brain neuropeptide genes expression were in line wi ... | 2016 | 26971175 |
| effects of melatonin injection or green-wavelength led light on the antioxidant system in goldfish (carassius auratus) during thermal stress. | we tested the mitigating effects of melatonin injections or irradiation from green-wavelength light-emitting diodes (leds) on goldfish (carassius auratus) exposed to thermal stress (high water temperature, 30 °c). the effects of the two treatments were assessed by measuring the expression and activity levels of the antioxidant enzymes, superoxide dismutase and catalase, plasma hydrogen peroxide, lipid hydroperoxide, and lysozyme. in addition, a comet assay was conducted to confirm that high wate ... | 2016 | 26965749 |
| cryopreservation of goldfish (carassius auratus) spermatozoa: effects of extender supplemented with taurine on sperm motility and dna damage. | amino acids, present in seminal plasma at high concentration, protect spermatozoa against cell damage during cryopreservation. | 2016 | 26964024 |
| thyroid disruption in male goldfish (carassius auratus) exposed to leachate from a municipal waste treatment plant: assessment combining chemical analysis and in vivo bioassay. | several classes of thyroid-disrupting chemicals (tdcs) have been found in refuse leachate, but the potential impacts of leachate on the thyroid cascade of aquatic organisms are yet not known. in this study, we chemically analyzed frequently reported tdcs, as well as conducted a bioassay, to evaluate the potential thyroid-disrupting effects of leachate. we used radioimmunoassay to determine the effects of leachate exposure on plasma 3,3',5-triiodo-l-thyronine (t3), 3,3',5,5'-l-thyroxine (t4), and ... | 2016 | 26950620 |
| anatomical features for the adequate choice of experimental animal models in biomedicine: i. fishes. | fish constitute the oldest and most diverse class of vertebrates, and are widely used in basic research due to a number of advantages (e.g., rapid development ex-utero, large-scale genetic screening of human disease). they represent excellent experimental models for addressing studies on development, morphology, physiology and behavior function in other related species, as well as informative analysis of conservation and diversity. although less complex, fish share many anatomical and physiologi ... | 2016 | 26925824 |
| phenolic endocrine disrupting chemicals in an urban receiving river (panlong river) of yunnan-guizhou plateau: occurrence, bioaccumulation and sources. | the objectives of this study were to track the occurrence, bioaccumulation and sources of phenolic endocrine disrupting chemicals (edcs) in a representative urban river (panlong river) of yunnan-guizhou plateau. it provided more comprehensive fundamental data for risk assessment and contamination control of phenolic edcs in aquatic environments. phenolic edcs, such as nonylphenol-di-ethoxylate (np2eo), nonylphenol-mono-ethoxylate (np1eo), 4-nonylphenol (4-np), bisphenol a (bpa), 4-cumylphenol (4 ... | 2016 | 26921547 |
| flow cytometric detection of oxidative dna damage in fish spermatozoa exposed to cadmium - short communication. | the aim of the present pilot study was to apply a flow cytometric assay, the so-called oxydna test, to determine the level of oxidative dna damage in fish spermatozoa exposed to different concentrations (0.01-10,000 mg/l) of cadmium. milt was collected from three randomly selected prussian carp (carassius auratus gibelio) males. oxidative dna damage was assessed with the oxydna kit and using flow cytometry. the ratio of oxydna-positive events increased significantly at higher cadmium concentrati ... | 2016 | 26919149 |
| influence of submarine groundwater discharge on (210)po and (210)pb bioaccumulation in fish tissues. | this study presents the results of the accumulation of (210)po and (210)pb in fish tissues and organs in a brackish-water marshland that is characterized by high concentrations of (222)rn and (226)ra supplied by submarine groundwater discharge (sgd). tissues and organs from cyprinus carpio, chelon labrosus and carassius auratus in the wetland were significantly enriched by both (210)pb and (210)po (up to 55 and 66 times, respectively) compared to blanks. the major input route of (210)pb and (210 ... | 2016 | 26913976 |
| bioconcentration and metabolism of ketoconazole and effects on multi-biomarkers in crucian carp (carassius auratus). | the tissue distribution, bioconcentration, metabolism and biological effects of the antifungal medication ketoconazole were investigated in fish, crucian carp (carassius auratus) were exposed to a series of nominal concentrations (0.2, 2 and 20 μg/l) for 14 days. the ultra-high performance liquid chromatography tandem triple quadrupole mass spectroscopy (uplc/ms/ms) analysis was used to determine the bioconcentration of ketoconazole and its metabolites in fish. the highest tissue concentration o ... | 2016 | 26901470 |
| evaluation of toxicity and genotoxicity of 2-chlorophenol on bacteria, fish and human cells. | due to the extensive use of chlorophenols (cps) in anthropogenic activities, 2-chlorophenol (2-cp), among other cps, can enter aquatic ecosystems and can be harmful to a variety of organisms, including bacteria, fish and humans, that are exposed directly and/or indirectly to such contaminated environments. based on the existing knowledge and in order to move a step forward, the purpose of this study is to investigate the toxic and mainly the genotoxic effects of 2-cp using a combination of bioas ... | 2016 | 26897408 |
| opisthorchis viverrini infections and associated risk factors in a lowland area of binh dinh province, central vietnam. | opisthorchiasis caused by opisthorchis viverrini is a major public health problem in the mekong basin in south east asia. it is associated with cholangiocarcinoma, a fatal cancer of the bile duct, which is very common in some areas of thailand and lao pdr. although there is evidence of opisthorchiasis in the central and southern provinces of vietnam, data are scarce and vietnam is often not considered an opisthorchiasis endemic area in the international literature. we conducted a cross-sectional ... | 2016 | 26872984 |
| stimulatory effect of the secretogranin-ll derived peptide secretoneurin on food intake and locomotion in female goldfish (carassius auratus). | secretoneurin (sn) is a conserved peptide derived by proteolytic processing from the middle domain of the ∼600 amino acid precursor secretogranin-ii (sgii). secretoneurin is widely distributed in secretory granules of endocrine cells and neurons and has important roles in reproduction as it stimulates luteinizing hormone release from the pituitary. a potential new role of sn in goldfish feeding is the subject of this study. firstly, we established that acute (26 h; p<0.0001) and short-term (72 h ... | 2016 | 26860475 |
| modulation of 17β-estradiol induced estrogenic responses in male goldfish (carassius auratus) by benzo[a]pyrene and ketoconazole. | the aquatic environment is challenged with complex mixtures of chemicals that may interact biochemically with each other in non-target aquatic organisms through a combination of actions, resulting in unpredictable mixture toxicity. this study focuses on the interactive effects of chemicals, including benzo(a)pyrene (bap) and ketoconazole (kcz), on 17β-estradiol (e2)-induced estrogenic responses in male goldfish (carassius auratus). the possible interactions between bap or kcz and e2 were investi ... | 2016 | 26825522 |
| tissue-specific expression of ghrelinergic and nucb2/nesfatin-1 systems in goldfish (carassius auratus) is modulated by macronutrient composition of diets. | the macronutrient composition of diets is a very important factor in the regulation of body weight and metabolism. several lines of research in mammals have shown that macronutrients differentially regulate metabolic hormones, including ghrelin and nesfatin-1 that have opposing effects on energy balance. this study aimed to determine whether macronutrients modulate the expression of ghrelin and the nucleobindin-2 (nucb2) encoded nesfatin-1 in goldfish (carassius auratus). fish were fed once dail ... | 2016 | 26805937 |
| isolation and characterization of hematopoietic stem cells in teleost fish. | despite 400 million years of evolutionary divergence, hematopoiesis is highly conserved between mammals and teleost fish. all types of mature blood cells including the erythroid, myeloid, and lymphoid lineages show a high degree of similarity to their mammalian counterparts at the morphological and molecular level. hematopoietic stem cells (hscs) are cells that are capable of self-renewal and differentiating into all hematopoietic lineages over the lifetime of an organism. the study of hscs has ... | 2016 | 26801099 |
| interactive effects of selected pharmaceutical mixtures on bioaccumulation and biochemical status in crucian carp (carassius auratus). | the aim of this study was to evaluate the interactive effects of fluoxetine (flu), roxithromycin (rox) and propranolol (prp) on the bioaccumulation and biochemical responses in the crucian carp carassius auratus. after 7 days of binary exposure (rox + flu and prp + flu), the addition of waterborne flu at nominal concentrations of 4, 20 and 100 μg l(-1) significantly increased the accumulation of rox and prp in fish livers in most cases, although elevated rox and prp bioaccumulation levels were n ... | 2016 | 26800487 |
| solution structure of the z-dna binding domain of pkr-like protein kinase from carassius auratus and quantitative analyses of the intermediate complex during b-z transition. | z-dna binding proteins (zbps) play important roles in rna editing, innate immune response and viral infection. structural and biophysical studies show that zbps initially form an intermediate complex with b-dna for b-z conversion. however, a comprehensive understanding of the mechanism of z-dna binding and b-z transition is still lacking, due to the absence of structural information on the intermediate complex. here, we report the solution structure of the zα domain of the zbp-containing protein ... | 2016 | 26792893 |
| morphometric and molecular characterization of dactylogyrus vastator and d. intermedius in goldfish (carassius auratus). | goldfish is known to be parasitized by at least seven species of dactylogyrus and considered as one of the most common hosts. dactylogyrus vastator and dactylogyrus intermedius are the dominant species living on the gills of goldfish. however, little information on morphometric characterization is available, which easily causes misidentification. the purpose of this study is to provide comprehensive morphometric and molecular characterization of d. vastator and d. intermedius collected form a fi ... | 2016 | 26779922 |
| estradiol and testosterone modulate the tissue-specific expression of ghrelin, ghs-r, goat and nucb2 in goldfish. | ghrelin, and nesfatin-1 (encoded by nucleobindin2/nucb2) are two metabolic peptides with multiple biological effects in vertebrates. while sex steroids are known to regulate endogenous ghrelin and nucb2 in mammals, such actions by steroids in fish remain unknown. this study aimed to determine whether estradiol (e2) and testosterone (t) affects the expression of preproghrelin, ghrelin/growth hormone secretagogue receptor (ghs-r), ghrelin o-acyl transferase (goat) and nucb2 in goldfish (carassius ... | 2016 | 26773340 |
| sialoglycoproteins prepared from the eggs of carassius auratus prevent bone loss by inhibiting the nf-κb pathway in ovariectomized rats. | in this study, we investigated the improvement of osteoporosis by sialoglycoproteins isolated from the eggs of carassius auratus (ca-sgp) in ovariectomized rats. ca-sgp was supplemented to ovariectomized sprague-dawley rats for 90 days. the results showed that ca-sgp treatment remarkably prevented the reduction of bone mass, improved cancellous bone structure and biochemical properties. ca-sgp also significantly decreased the serum contents of trap, cath-k, mmp-9, dpd, ctx-1, ca, and p. mechanis ... | 2016 | 26765587 |
| responses of antioxidant defense system to polyfluorinated dibenzo-p-dioxins (pfdds) exposure in liver of freshwater fish carassius auratus. | in this study, we evaluated the toxicity of ten polyfluorinated dibenzo-p-dioxins (pfdds) congeners to freshwater fish carassius auratus, by determining the antioxidative responses and lipid peroxidation in the liver after the fish were injected with two different concentrations (10 and 100 µmol/kg) of individual pfdds for 3 and 14 days. the results showed that oxidative stress was obviously induced in some pfdds-treated groups, as implied by the significantly inhibited antioxidants levels (supe ... | 2016 | 26761781 |
| development of monoclonal antibodies against polar filaments and spore valves of myxobolus honghuensis (myxosporea: bivalvulida). | myxobolus honghuensis infects the pharynx of allogynogenetic gibel carp carassius auratus gibelio (bloch) and can cause high mortality. only morphology-based diagnostic methods are currently available for clinical samples, but these methods are laborious and have low efficiency of detection. to overcome this problem, we designed a more sensitive diagnostic method. two monoclonal antibodies (mabs 1c7 and 3b7) were prepared by immunizing mice with soluble protein from sonicated m. honghuensis spor ... | 2016 | 26758653 |
| insulin-like growth factor-binding protein-1 (igfbp-1) in goldfish, carassius auratus: molecular cloning, tissue expression, and mrna expression responses to periprandial changes and cadmium exposure. | in this study, the cdna encoding insulin-like growth factor-binding protein-1 (igfbp-1) was cloned from the liver of goldfish (carassius auratus). the obtained goldfish igfbp-1 cdna sequence was 1037 bp in length and had an open reading frame of 789 bp encoding a predicted polypeptide of 262 amino acid residues. igfbp-1 transcript was detected in all tested central nervous and peripheral tissues. the relatively higher levels of igfbp-1 mrna were observed in the liver, gill, kidney, heart, spleen ... | 2016 | 26753895 |
| genotoxicity of water contaminants from the basin of lake sevan, armenia evaluated by the comet assay in gibel carp (carassius auratus gibelio) and tradescantia bioassays. | combination of bioassays and chemical analysis was applied to determine the genotoxic/mutagenic contamination in four different sites of the basin of lake sevan in armenia. water genotoxicity was evaluated using the single cell gel electrophoresis technique (comet assay) in erythrocytes of gibel carp (carassius auratus gibelio), tradescantia micronucleus (trad-mcn) and tradescantia stamen hair mutation (trad-shm) assays. significant inter-site differences in the levels of water genotoxicity acco ... | 2016 | 26739952 |
| the complete mitochondrial genome of carassius auratus var. high back crucian carp (cypriniformes: cyprinidae). | in this study, the complete mitochondrial genome sequence of carassius auratus var. high back crucian carp (c. auratus var.) was determined (genbank accession number is kt756205). the mitochondrial genome sequence of c. auratus var. was a circular molecule with 16 581 bp in length, and contained 37 typical animal mitochondrial genes, including 2 ribosomal rna genes, 13 protein-coding genes, 22 transfer rna genes and a control region (d-loop). four nucleotide compositions and their relative propo ... | 2017 | 26709972 |
| prevalence of argulus foliaceus in ornamental fishes [goldfish (carassius auratus) and koi (cyprinus carpio)] in kerman, southeast of iran. | the genus argulus (crustacea: branchiura), or fish louse, are common parasites of freshwater fish. this parasite have a direct life cycles and mature females leave the host and lay several hundred eggs on vegetation and various objects in the water. it caused pathological changes due to direct tissue damage and secondary infections. besides the damage and stress caused by argulus itself, one of the main worries for fish producers are the associated secondary infestations and infections that can ... | 2015 | 26688652 |
| mass spectrometry-assisted confirmation of the inability of dipeptidyl peptidase-4 to cleave goldfish peptide yy(1-36) and the lack of anorexigenic effects of peptide yy(3-36) in goldfish (carassius auratus). | dipeptidyl peptidase-4 (dpp4) is a serine protease of great interest because it has been shown to modulate the activity of several peptidergic factors including peptide yy (pyy) and glucagon-like peptide-1/2. while pyy(1-36) is orexigenic in mammals, pyy(3-36) recently garnered interest as a potent anorexigen. in silico phylogenetic analysis found that the dpp4 cleavage sites are absent in fish pyy sequences. however, no studies were conducted to show that indeed pyy(3-36) is not produced by dpp ... | 2016 | 26676513 |
| comparative proteomic, physiological, morphological, and biochemical analyses reveal the characteristics of the diploid spermatozoa of allotetraploid hybrids of red crucian carp (carassius auratus) and common carp (cyprinus carpio). | the generation of diploid spermatozoa is essential for the continuity of tetraploid lineages. the dna content of diploid spermatozoa from allotetraploid hybrids of red crucian carp and common carp was nearly twice as great as that of haploid spermatozoa from common carp, and the durations of rapid and slow progressive motility were longer. we performed comparative proteomic analyses to measure variations in protein composition between diploid and haploid spermatozoa. using two-dimensional electr ... | 2016 | 26674567 |
| bioconcentration, metabolism and effects of diphenhydramine on behavioral and biochemical markers in crucian carp (carassius auratus). | diphenhydramine (dph), an antihistamine used to alleviate human allergies, is widespread in aquatic environments. however, little is known about the biochemical and behavioral effects of dph on non-target aquatic animals. in the present study, the tissue distribution, bioconcentration, metabolism, biochemical and behavioral effects were investigated in crucian carp (carassius auratus) exposed to various concentrations of dph (0.84, 4.23, 21.7 and 112 μg l(-1)) for 7d. dph can accumulate in cruci ... | 2016 | 26657385 |
| antioxidant defenses and histological changes in carassius auratus after combined exposure to zinc and three multi-walled carbon nanotubes. | with the increasing applications of carbon nanotubes (cnts) worldwide, considerable concerns have been raised regarding their inevitable releases into natural waters and ecotoxicity. it was supposed that cnts may interact with some existing pollutants like zinc in aquatic systems and exhibit different effects when compared with their single treatments. however, data on their possible combined toxicity on aquatic species are still lacking. moreover, the interactions of zn with different functiona ... | 2016 | 26655435 |
| the chromosomal constitution of fish hybrid lineage revealed by 5s rdna fish. | the establishment of the bisexual fertile fish hybrid lineage including the allodiploid and allotetraploid hybrids, from interspecific hybridization of red crucian carp (carassius auratus red var. 2n = 100, 2n = aa) (♀) × common carp (cyprinus carpio l. 2n = 100, 2n = bb) (♂), provided a good platform to investigate genetic relationship between the parents and their hybrid progenies. | 2015 | 26635010 |
| carbon and silver nanoparticles in the fight against the filariasis vector culex quinquefasciatus: genotoxicity and impact on behavioral traits of non-target aquatic organisms. | mosquito-borne diseases represent a deadly threat for millions of people worldwide. the culex genus, with special reference to culex quinquefasciatus, comprises the most common vectors of filariasis across urban and semi-urban areas of asia. in recent years, important efforts have been conducted to propose green-synthesized nanoparticles as a valuable alternative to synthetic insecticides. however, the mosquitocidal potential of carbon nanoparticles has been scarcely investigated. in this study, ... | 2016 | 26614358 |
| effects of semen extender supplemented with l-methionine and packaging methods (straws and pellets) on post-thaw goldfish (carassius auratus) sperm quality and dna damage. | amino acids protect spermatozoa against cell damage during cryopreservation due to have antioxidant property and found in seminal plasma at high concentration. | 2015 | 26574681 |
| characterization and mosquitocidal potential of neem cake-synthesized silver nanoparticles: genotoxicity and impact on predation efficiency of mosquito natural enemies. | mosquitoes (diptera: culicidae) serve as important vectors for a wide number of parasites and pathogens of huge medical and veterinary importance. aedes aegypti is a primary dengue vector in tropical and subtropical urban areas. there is an urgent need to develop eco-friendly mosquitocides. in this study, silver nanoparticles (agnp) were biosynthesized using neem cake, a by-product of the neem oil extraction from the seed kernels of azadirachta indica. agnp were characterized using a variety of ... | 2016 | 26573518 |
| pharmacokinetics of sarafloxacin in allogynogenetic silver crucian carp, carassius auratus gibelio. | the pharmacokinetic properties of sarafloxacin were investigated after single intravenous (i.v.) and oral (p.o.) administration of 10 mg/kg body weight (b.w.) in allogynogenetic silver crucian carp at 24-26 °c. the plasma concentrations of sarafloxacin were determined by high-performance liquid chromatography. after i.v. administration, the plasma concentration-time data were described by an open two-compartment model. the elimination half-life (t(1/2β)) was estimated to be 22.58 h. the volume o ... | 2016 | 26563280 |
| herpesviral hematopoietic necrosis in goldfish in switzerland: early lesions in clinically normal goldfish (carassius auratus). | cyprinid herpesvirus 2 is a pathogen of goldfish, inducing a disease referred to as herpesviral hematopoietic necrosis. the disease is described so far in japan, north america, taiwan, australia, the united kingdom, and recently also italy. here the authors describe histologic lesions in clinically affected fish in comparison with clinically normal but virus dna-positive goldfish in switzerland. while necrosis or enhanced single-cell necrosis in the hematopoietic tissue in the pronephros or meso ... | 2016 | 26553521 |
| goldfish (carassius auratus l.) as a model system to study the growth factors, receptors and transcription factors that govern myelopoiesis in fish. | the process of myeloid cell development (myelopoiesis) in fish has mainly been studied in three cyprinid species: zebrafish (danio rerio), ginbuna carp (carassius auratus langsdorfii) and goldfish (c. auratus, l.). our studies on goldfish myelopoiesis have utilized in vitro generated primary kidney macrophage (pkm) cultures and isolated primary kidney neutrophils (pkns) cultured overnight to study the process of macrophage (monopoiesis) and neutrophil (granulopoiesis) development and the key gro ... | 2016 | 26546240 |
| molecular and functional characterization of goldfish (carassius auratus l.) serum amyloid a. | quantitative expression analysis of goldfish saa revealed the highest mrna levels in the kidney, spleen and intestine with lower mrna levels in muscle and liver. goldfish saa was differentially expressed in goldfish immune cells with highest mrna levels observed in neutrophils. to functionally assess goldfish saa, recombinant protein (rgsaa) was generated by prokaryotic expression and functionally characterized. monocytes and macrophages treated with rgsaa exhibited differential gene expression ... | 2015 | 26523984 |
| in situ localization and rhythmic expression of ghrelin and ghs-r1 ghrelin receptor in the brain and gastrointestinal tract of goldfish (carassius auratus). | ghrelin is a gut-brain peptide hormone, which binds to the growth hormone secretagogue receptor (ghs-r) to regulate a wide variety of biological processes in fish. despite these prominent physiological roles, no studies have reported the anatomical distribution of preproghrelin transcripts using in situ hybridization in a non-mammalian vertebrate, and its mapping within the different encephalic areas remains unknown. similarly, no information is available on the possible 24-h variations in the e ... | 2015 | 26506093 |
| proteomic analysis of cytoskeleton proteins in fish. | in this chapter, we describe laboratory protocols for rearing fish and a simple and efficient method of extracting and identifying pathogen and host proteins that may be involved in entry and replication of commercially important fish viruses. we have used the common carp (cyprinus carpio l.) and goldfish (cyprinus auratus) as a model system for studies of proteins involved in viral entry and replication. the chapter describes detailed protocols for maintenance of carp, cell culture, antibody pu ... | 2016 | 26498797 |
| recombinant saccharomyces cerevisiae serves as novel carrier for oral dna vaccines in carassius auratus. | oral delivery of dna vaccines represents a promising vaccinating method for fish. recombinant yeast has been proved to be a safe carrier for delivering antigen proteins and dnas to some species in vivo. however, whether recombinant yeast can be used to deliver functional dnas for vaccination to fish is still unknown. in this study, red crucian carp (carassius auratus) was orally administrated with recombinant saccharomyces cerevisiae harboring cmv-egfp expression cassette. on day 5 post the firs ... | 2015 | 26481518 |
| effects of repeated handling and air exposure on the immune response and the disease resistance of gibel carp (carassius auratus gibelio) over winter. | high mortalities and suppressed immune functions of farmed fish over winter are the universal problems in aquaculture. it is necessary to improve the immune response and disease resistance in the overwintering fish. a recent study suggested that repeated handling increased innate immune mechanisms and disease resistance in senegalese sole. therefore, the present study evaluated the hypothesis that appropriate repeated handling could compromise the immune depression and increase the disease resis ... | 2015 | 26481516 |
| ectocommensal and ectoparasites in goldfish carassius auratus (linnaeus, 1758) in farmed in the state of são paulo. | concomitant infections by several parasitic genera are rare, very debilitating and often lethal to fish reared under commercial breeding conditions. were describe a multiple and concurrent parasite infestation in cultured goldfish carassius auratus with skin damage (nodules and/or ulceration). fish with skin lesions underwent necropsy, and the skin and gills were scraped and examined. histopathological examination with hematoxylin-eosin and giemsa stain, and an ultrastructure study using transmi ... | 2015 | 26444060 |
| establishment from the snout and kidney of goldfish, carassius auratus, of two new cell lines and their susceptibility to infectious pancreatic necrosis virus. | goldfish carassius auratus are commonly used in scientific research and have a significant economic value in the pet trade. in this study, two cell lines were established from the snout and kidney tissue of goldfish, in order to create a biological monitoring tool for viral diseases. cell lines were optimally maintained at 25 °c in m199 medium supplemented with 15-20 % fetal bovine serum. chromosomal analysis indicated that both cell lines remained diploid, with a mean chromosomal count of 100. ... | 2016 | 26419476 |
| effects of body chemical components on the allometric scaling of the resting metabolic rate in four species of cyprinids. | metabolic scaling is hypothesized to be affected by ontogenetic changes in the body chemical composition because several body components do not consume significant amounts of oxygen but contribute more to body mass (m) as a fish grows. we tested this hypothesis by assessing the mass scaling of the resting metabolic rate (rmr) and body compositions of lipid, protein, ash, and water in four species of cyprinids, hypophthalmichthys molitrix (m range 9.5-218.3 g), cyprinus carpio (m range 14.6-226.9 ... | 2016 | 26407956 |
| dactylogyrus intermedius parasitism enhances flavobacterium columnare invasion and alters immune-related gene expression in carassius auratus. | the monogenean dactylogyrus intermedius and the bacterium flavobacterium columnare are 2 common pathogens in aquaculture. the objective of the present study was to examine the effect of prior parasitism by d. intermedius on the susceptibility of goldfish to f. columnare and to explore the potential immune mechanisms related to the parasite infection. a f. columnare challenge trial was conducted between d. intermedius-parasitized and non-parasitized goldfish. the f. columnare load in gill, kidney ... | 2015 | 26378404 |
| correlations of gut microbial community shift with hepatic damage and growth inhibition of carassius auratus induced by pentachlorophenol exposure. | goldfish (carassius auratus) were exposed to 0-100 μg/l pentachlorophenol (pcp) for 28 days to investigate the correlations of fish gut microbial community shift with the induced toxicological effects. pcp exposure caused accumulation of pcp in the fish intestinal tract in a time- and dose-dependent manner, while hepatic pcp reached the maximal level after a 21 day exposure. under the relatively higher pcp stress, the fish body weight and liver weight were reduced and hepatic cat and sod activit ... | 2015 | 26378342 |
| ultrasonographic and pathologic study of schwannoma in a goldfish (carassius auratus). | an adult goldfish (carassius auratus) developed an exophitic, multinodular, mass in the dorsal region involving the fin. the clinical and pathologic approach included ultrasonography, cytology, histopathology, immunohistochemistry, and ultrastructural evaluation. b-mode and color doppler ultrasonographic findings showed an oval, well-defined mass with slightly inhomogeneous parenchymatous echotexture associated with an intense intralesional vascularization. cytology and histology revealed neopla ... | 2015 | 26356489 |
| human chorionic gonadotropin promotes expression of protein absorption factors in the intestine of goldfish (carassius auratus). | protein use is crucial for the ovulation and spawning of fish. currently, limited information is available regarding the expression of protein absorption factors during the breeding seasons of teleosts and thus how various proteins involved in this process is not well-understood. the expression of cdx2, creb, gluatamate dehydrogenase, lat2, aminopeptidase n, pept1, and sp1 were significantly elevated from the non-breeding season to the breeding season in female goldfish, and all proteins except ... | 2015 | 26345757 |
| response of vitamins a, e, hematological and serum biochemical markers in crucian carp (carassius auratus gibelio) exposed to environmental pb(2+) and cd(2+). | toxicity of pb(2+) and cd(2+) is a widespread issue in the world; however, few studies have been conducted to understand their effect at environmentally realistic concentration in a mixture. in the present study, crucian carp was exposed to pb(2+) (30 µg·l(-1)), cd(2+) (100 µg·l(-1)) and their mixture (30+100 µg·l(-1)) for 96 h and 21 d period to assess changes in the liver and muscle vitamin a and e content, and hematological and serum biochemical parameters. the results indicated significant d ... | 2015 | 26336655 |
| expression patterns of toll-like receptors in natural triploid carassius auratus after infection with aeromonas hydrophila. | toll-like receptors (tlrs) play the important roles in the innate immune system. in the present study, we have cloned fragments of 13 members of tlr family in natural triploid qihe crucian carp (c. auratus) and investigated the expression profiles of these tlrs in spleen and head kidney at different time points after a. hydrophila infection. the results showed that the expressions of certain tlrs including tlr4, tlr5, and tlr22 were significantly up-regulated after infection, and the expression ... | 2015 | 26319889 |
| isolation, characterization, and tissue-specific expression of gaba a receptor α1 subunit gene of carassius auratus gibelio after avermectin treatment. | carassius auratus gibelio has been widely cultivated in fish farms in china, with avermectin (avm) being used to prevent parasite infection. recently, avm was found to pass through the carassius auratus gibelio blood-brain barrier (bbb). although avm acts mainly through a gaba receptor and specifically the α1 subunit gene, the most common isoform of the gaba a receptor, which is widely expressed in brain neurons and has been studied in other fish, carassius auratus gibelio gaba a receptor α1 sub ... | 2016 | 26318443 |
| postembryonic staging of wild-type goldfish, with brief reference to skeletal systems. | artificial selection of postembryonic features is known to have established morphological variation in goldfish (carassius auratus). although previous studies have suggested that goldfish and zebrafish are almost directly comparable at the embryonic level, little is known at the postembryonic level. | 2015 | 26316229 |
| neutrophil contributions to the induction and regulation of the acute inflammatory response in teleost fish. | neutrophils are essential to the acute inflammatory response, where they serve as the first line of defense against infiltrating pathogens. we report that, on receiving the necessary signals, teleost (carassius auratus) neutrophils leave the hematopoietic kidney, enter into the circulation, and dominate the initial influx of cells into a site of inflammation. unlike mammals, teleost neutrophils represent <5% of circulating leukocytes during periods of homeostasis. however, this increases to near ... | 2016 | 26292979 |
| in vitro characteristics of cyprinid herpesvirus 2: effect of kidney extract supplementation on growth. | herpesviral hematopoietic necrosis caused by goldfish hematopoietic necrosis virus (now identified as cyprinid herpesvirus 2, cyhv-2) has contributed to economic losses in goldfish carassius auratus culture and is becoming a major obstacle in prussian carp c. gibelio aquaculture in china. several reports have described difficulties in culturing the virus, with the total loss of infectivity within several passages in cell culture. we succeeded in propagating cyhv-2 with a high infectious titer in ... | 2015 | 26290507 |
| nanoparticles in the fight against mosquito-borne diseases: bioactivity of bruguiera cylindrica-synthesized nanoparticles against dengue virus den-2 (in vitro) and its mosquito vector aedes aegypti (diptera: culicidae). | mosquitoes are blood-feeding insects serving as the most important vectors for spreading human pathogens and parasites. dengue is a viral disease mainly vectored through the bite of aedes mosquitoes. its transmission has recently increased in urban and semi-urban areas of tropical and subtropical regions worldwide, becoming a major international public health concern. there is no specific treatment for dengue. its prevention and control solely depend on effective vector control measures. mangrov ... | 2015 | 26290219 |
| comparison of the toxicokinetics and bioaccumulation potential of mercury and polychlorinated biphenyls in goldfish (carassius auratus). | both mercury (hg) and polychlorinated biphenyls (pcbs) demonstrate food web biomagnification in aquatic ecosystems, yet their toxicokinetics have not been simultaneously contrasted within a common fish species. this study quantifies uptake and elimination rates of hg and pcbs in goldfish. fish were exposed to contaminated food containing pcbs and hg to determine dietary chemical assimilation efficiencies (aes) and elimination coefficients (ktot). to test first-order kinetics, three exposure regi ... | 2015 | 26287735 |
| multistress effects on goldfish (carassius auratus) behavior and metabolism. | crossed effects between climate change and chemical pollutions were identified on community structure and ecosystem functioning. temperature rising affects the toxic properties of pollutants and the sensitiveness of organisms to chemicals stress. inversely, chemical exposure may decrease the capacity of organisms to respond to environmental changes. the aim of our study was to assess the individual and crossed effects of temperature rising and pesticide contamination on fish. goldfish, carassius ... | 2016 | 26272290 |
| myxozoans and our dinner table: pathogenicity studies of myxobolus honghuensis (myxosporea: bivalvulida) using a suckling mice model. | myxobolus honghuensis parasitizes the pharynx of the allogynogenetic gibel carp carassius auratus gibelio (bloch), an economically important food fish with large consumption rates in china. the fact that it is hard to detect the cysts formed by m. honghuensis without euthanizing the fish and the spores can reach the consumers has prompted us to investigate the effect of m. honghuensis on conventional, immunocompetent balb/c suckling mice, in order to consider the possible pathological effects in ... | 2015 | 26258259 |
| a nonclassical mhc class i u lineage locus in zebrafish with a null haplotypic variant. | three sequence lineages of mhc class i genes have been described in zebrafish (danio rerio): u, z, and l. the u lineage genes encoded on zebrafish chromosome 19 are predicted to provide the classical function of antigen presentation. this mhc class i locus displays significant haplotypic variation and is the only mhc class i locus in zebrafish that shares conserved synteny with the core mammalian mhc. here, we describe two mhc class i u lineage genes, mhc1ula and mhc1uma, that map to chromosome ... | 2015 | 26254596 |
| nesfatin-1 as a new positive inotrope in the goldfish (carassius auratus) heart. | the hypothalamic neuropeptide nesfatin-1 is present in both mammals and teleosts in which it elicits anorexigenic effects. in mammals, nesfatin-1 acts on the heart by inducing negative inotropism and lusitropism, and cardioprotection against ischemic damages. we evaluated whether in teleosts, nesfatin-1 also influences cardiac performance. in the goldfish (carassius auratus), mature, fully processed nesfatin-1 was detected in brain, gills, intestine and skeletal muscle, but not in the cardiac ve ... | 2015 | 26248227 |
| physiological and condition-related traits in the gynogenetic-sexual carassius auratus complex: different investments promoting the coexistence of two reproductive forms? | carassius auratus complex is an extraordinary species complex including the diploid and polyploid forms exhibiting asexual and sexual reproduction modes. the coexistence of both forms in the same habitats is currently reported. the stable coexistence of asexual and sexual forms assumes some disadvantages for asexuals that balance the costs of sex. in our study, we hypothesized and tested the differences in physiological (including heamatological and immunological), growth-related, condition-rela ... | 2015 | 26245328 |
| induced all-female autotriploidy in the allotetraploids of carassius auratus red var. (♀) × megalobrama amblycephala (♂). | following activation by uv-irradiated bsb sperm, the fertilized eggs of tetraploid hybrids (abbreviated as 4nf1) (4n = 148, aabb) of carassius auratus red var. (abbreviated as rcc) (2n = 100, aa) (♀) × megalobrama amblycephala (abbreviated as bsb) (2n = 48, bb) (♂) developed into normal live gynogenetic offspring without chromosome doubling treatment. some of these were autotriploids with three sets of red crucian carp chromosomes (abbreviated as g1) (3n = 150, aaa). g1 were all-females, and can ... | 2015 | 26242753 |
| short-term exposure to l-type calcium channel blocker, verapamil, alters the expression pattern of calcium-binding proteins in the brain of goldfish, carassius auratus. | the influx of calcium ions (ca(2+)) is responsible for various physiological events including neurotransmitter release and synaptic modulation. the l-type voltage dependent calcium channels (l-type vdccs) transport ca(2+) across the membrane. calcium-binding proteins (cabps) bind free cytosolic ca(2+) and prevent excitotoxicity caused by sudden increase in cytoplasmic ca(2+). the present study was aimed to understand the regulation of expression of neuronal cabps, namely, calretinin (cr) and par ... | 2015 | 26215640 |
| (1)h nmr based metabolomics approach to study the toxic effects of dichlorvos on goldfish (carassius auratus). | dichlorvos (ddvp), one of the most widely used organophosphorus pesticides (ops), has caused serious pollution in environment. in this study, (1)h nuclear magnetic resonance (nmr) based metabolomics approach combined with histopathological and immunohistochemical examination, and biochemical assays were used to investigate toxicities of ddvp on goldfish (carassius auratus). after 10 days' exposure of ddvp at three dosages of 5.18, 2.59 and 1.73 mg/l, goldfish tissues (gill, brain, liver and kidn ... | 2015 | 26210017 |
| art, fisheries and ethnobiology. | nature is perceived in a variety of forms, and the perception of nature can also be expressed in different ways. local art may represent the perception of nature by humans. it can embody perception, imagination and wisdom. local art, in particular, reflects how people interact with nature. for example, when studying the representation of fish by different cultures, it is possible to access information on the fish species found in the environment, on its relative importance, and on historical eve ... | 2015 | 26187281 |
| first detection of cyprinid herpesvirus 2 (cyhv-2) in goldfish (carassius auratus) in france. | massive mortalities of carassius auratus (l.) occurred in a farm in france during summer 2014. fish presented anorexia, loss of scales and large amounts of mucus on the gills. necrosis of the distal tip of the filament and the lamellae, combined with fusion of the lamellae, was observed, as well as necrosis in the hematopoietic organs and in the digestive tract. the histological examination led to hypothesize the implication of a virus in the mortality. the presence of cyprinid herpesvirus 2 (cy ... | 2016 | 26173014 |
| anatomical distribution and daily profile of gper1b gene expression in brain and peripheral structures of goldfish (carassius auratus). | the functional organization of the circadian system and the location of the main circadian oscillators vary through phylogeny. present study investigates by in situ hybridization the anatomical location of the clock gene gper1b in forebrain and midbrain, pituitary, and in two peripheral locations, the anterior intestine and liver, in a teleost fish, the goldfish (carassius auratus). moreover, the daily expression profiles of this gene were also studied by quantitative real time-pcr. goldfish wer ... | 2015 | 26171989 |
| effects of dietary supplementation with astaxanthin and β-carotene on the semen quality of goldfish (carassius auratus). | this study was conducted to investigate the effects of two carotenoids (astaxanthin and β-carotene) on the sperm quality of goldfish carassius auratus (linnaeus, 1758). for this purpose, six diets containing concentrations of 50, 100, and 150 mg/kg of synthetic astaxanthin and β-carotene were added to a basic carp diet. one group of fish was also fed with a control diet (no added carotenoids). osmolality, spermatocrit value, and sperm concentration significantly increased in the treatment supple ... | 2015 | 26166170 |
| toxicity of environmental gesagard to goldfish may be connected with induction of low intensity oxidative stress in concentration- and tissue-related manners. | prometryn is a selective herbicide commonly used in agriculture as the commercial preparation, gesagard. goldfish (carassius auratus) exposure for 96h to 0.2, 1, or 5mgl(-1) gesagard 500fw (corresponding to 0.1, 0.5, and 2.5mgl(-1) of prometryn) on indices of oxidative stress (lipid peroxides, protein carbonyls, and thiol content) and activities of antioxidant and related enzymes in gills, liver, and kidney was studied. gills appeared to be the most resistant to gesagard treatment, reacting to o ... | 2015 | 26133465 |
| morphological and molecular characterization of thelohanellus hoffmanni sp. nov. (myxozoa) infecting goldfish carassius auratus auratus. | a new species of the genus thelohanellus kudo, 1933 (myxosporea, bivalvulida) was isolated from the fins of goldfish carassius auratus auratus (linnaeus 1758). the fish had been imported from china by an austrian retailer. nodules from the margins of the fins contained pyriform myxospores with a singular polar capsule. in valvular view, the spores measured 12.2 µm in length and 6.4 µm in width. in sutural view, the thickness was 2.9 µm. the polar capsule measured 4.2 × 3.1 µm and contained a pol ... | 2015 | 26119298 |
| goldfish carassius auratus susceptibility to viral hemorrhagic septicemia virus genotype ivb depends on exposure route. | we assessed the susceptibility of goldfish carassius auratus to infection by genotype ivb of the viral hemorrhagic septicemia virus. goldfish were infected by intraperitoneal injections of 106 plaque-forming units (pfu) fish-1, single bath exposure of 105 pfu ml-1 for 24 h, or consumption of 0.4 g of commercial fish feed soaked in 107 pfu per 8 fish. the mortality rate of intraperitoneal-infected goldfish was 10 to 32%, although the virus was detected by quantitative rt-pcr in 77% (65/84) of the ... | 2015 | 26119297 |
| the analysis of the acute phase response during the course of trypanosoma carassii infection in the goldfish (carassius auratus l.). | the expression of genes encoding the acute phase proteins (app) during the course of trypanasoma carassii infection in the goldfish was determined using quantitative pcr. significant changes in the mrna levels of ceruloplasmin (cp), c-reactive protein (crp), transferrin (tf), hemopexin (hx) and serum amyloid a (saa) were observed in the kidney, liver and spleen at various days post infection (dpi). of the five acute phase protein genes examined, crp and saa exhibited the highest expression in th ... | 2015 | 26116443 |
| cyhv-2 orf104 activates the p38 mapk pathway. | cyprinid herpesvirus 2 (cyhv-2) is the pathogen responsible for herpesviral hematopoietic necrosis disease, which causes huge losses on aquaculture. so far the studies of cyhv-2 mainly focus on the identification and detection of this virus, but little is known about the role of specific cyhv-2 genes in the infection process. based on the genomic information, cyhv-2 orf104 encodes a kinase-like protein, which is highly conserved among the three cyhvs. our study was initiated to investigate the r ... | 2015 | 26072141 |
| hepatotoxicity of herbicide sencor in goldfish may result from induction of mild oxidative stress. | the effects of 96 h exposure to 7.14, 35.7, or 71.4 mg l(-1) of sencor were studied on liver and plasma parameters in goldfish, carassius auratus l. goldfish exposure to 71.4 mg l(-1) of sencor for 96 h resulted in a decrease in glucose concentrations in plasma and liver by 55%, but did not affect liver glycogen levels. an increase in the activity of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase (by 24-27%, 32-72%, and 87-102%, respectively) occurred in plasma of ... | 2015 | 26071809 |
| biological fate and effects of propranolol in an experimental aquatic food chain. | the aim of this study was to evaluate the trophic transfer of the β-blocker propranolol (prp) in an experimental aquatic food chain involving the green algae scenedesmus obliquus, the water flea daphnia magna and the crucian carp carassius auratus, as well as the metabolism and effects of prp in the liver of crucian carp. after a 48 h prp aqueous exposure for algae, with a subsequent 48 h dietary exposure for daphnia and an 8d dietary exposure for crucian carp, prp was observed in each trophic l ... | 2015 | 26057722 |
| ionoregulatory aspects of the osmorespiratory compromise during acute environmental hypoxia in 12 tropical and temperate teleosts. | in the traditional osmorespiratory compromise, as seen in the hypoxia-intolerant freshwater rainbow trout (oncorhynchus mykiss), the branchial modifications that occur to improve o2 uptake during hypoxia result in unfavorable increases in the fluxes of ions and water. however, at least one hypoxia-tolerant freshwater species, the amazonian oscar (astronotus ocellatus), shows exactly the opposite: decreased branchial flux rates of ions, water, and nitrogenous wastes during acute hypoxia. in order ... | 2015 | 26052633 |
| two cholecystokinin receptor subtypes are identified in goldfish, being the cckar involved in the regulation of intestinal motility. | cholecystokinin (cck) plays a key role in the digestive physiology of vertebrates. however, very little is known about the role of cck on intestinal functions in fish. the present study identifies two cck receptor subtypes in a stomachless teleost, the goldfish (carassius auratus), and investigates by using an in vitro system their involvement mediating the effects of the sulfated octapeptide of cck (cck-8s) on the motility of isolated proximal intestine. partial-length mrnas encoding two cck re ... | 2015 | 26051613 |
| morphological and molecular characterization of actinosporeans infecting oligochaete branchiura sowerbyi from chinese carp ponds. | we surveyed the actinosporean stages of fish myxosporeans at fish farms in jiangsu province, china, from 2011 to 2014. during the surveys, we identified 7 actinosporean types from 4 collective groups: echinactinomyxon (1 type), triactinomyxon (1 type), aurantiactinomyxon (1 type), and neoactinomyxum (4 types), released by the oligochaete branchiura sowerbyi. the morphological characteristics and dna sequences of these types are described here. based on 18s rdna sequence analysis, the actinospore ... | 2015 | 26036829 |
| development of a lipovitellin-based sandwich elisa for quantification of vitellogenin in surface mucus and plasma of goldfish (carassius auratus). | goldfish (carassius auratus) vitellogenin (vtg) is an efficient biomarker for estrogen contamination in aquatic environments. in this study, vtg and lipovitellin (lv) were purified from the plasma of 17β-estradiol (e2)-induced male goldfish and unfertilized eggs of females, and were used to generate polyclonal antibodies against vtg (anti-vtg) and lv (anti-lv), respectively. sds-page and western blot were performed to confirm the specificity of the two antibodies and the immunological similarity ... | 2015 | 26036419 |
| hepatic oxidative stress biomarker responses in freshwater fish carassius auratus exposed to four benzophenone uv filters. | benzophenone (bp) type uv filters are widely used in many personal care products to protect human from uv irradiation. despite the estrogenic potencies to fish and the environmental occurrences of bp derivatives in aquatic systems, little information is available regarding their effects on the antioxidant defense system in fish. in this work, the oxidative stress induced in livers of carassius auratus was assessed using four biomarkers. the integrated biomarker response (ibr) was applied to asse ... | 2015 | 25996523 |
| survey of parasitic fauna of different ornamental freshwater fish species in iran. | parasitic diseases are harmful and limiting factors in breeding and rearing ornamental fish industry. in this study, 400 apparently healthy ornamental fishes from five species (each species 80 specimens) including: goldfish (carassius auratus), guppy (poecilia reticulate), angelfish (pterophyllum scalare), discus (symphsodon discus) and sailfin mollies (poecilia latipinna) was obtained from a local ornamental fish farm in the north of iran during 2011 to 2012. the primary purpose of this study w ... | 2015 | 25992255 |
| physiological and molecular responses of the goldfish (carassius auratus) kidney to metabolic acidosis, and potential mechanisms of renal ammonia transport. | relative to the gills, the mechanisms by which the kidney contributes to ammonia and acid-base homeostasis in fish are poorly understood. goldfish were exposed to a low ph environment (ph 4.0, 48 h), which induced a characteristic metabolic acidosis and an increase in total plasma [ammonia] but reduced plasma ammonia partial pressure (pnh3). in the kidney tissue, total ammonia, lactate and intracellular ph remained unchanged. the urinary excretion rate of net base under control conditions change ... | 2015 | 25987732 |
| expression characterization, genomic structure and function analysis of fish ubiquitin-specific protease 18 (usp18) genes. | in mammals, usp18 (ubiquitin-specific protease 18) is an interferon (ifn) inducible protein and plays a role in regulation of ifn response upon viral infection. in this study, we first cloned a usp18 homologous gene from virally-infected crucian carp (carassius auratus) blastula embryonic (cab) cells, and later found in other fish species including zebrafish. all fish usp18 genes have 10 exons and 9 introns comparable to 11 exons and 10 introns in non-fish vertebrates. expression analysis reveal ... | 2015 | 25981749 |
| acute and chronic toxicity of tetrabromobisphenol a to three aquatic species under different ph conditions. | tetrabromobisphenol a (tbbpa) is a well-known brominated flame retardant. it has been detected in the environment and shows high acute toxicity to different organisms at high concentrations. in this work, the effects of ph and dimethyl sulfoxide (dmso) on the acute toxicity of tbbpa to daphnia magna and limnodrilus hoffmeisteri were tested, and the oxidative stress induced by tbbpa in livers of carassius auratus was assessed using four biomarkers. the integrated biomarker response (ibr) was appl ... | 2015 | 25980965 |
| increase in telencephalic dopamine and cerebellar norepinephrine contents by hydrostatic pressure in goldfish: the possible involvement in hydrostatic pressure-related locomotion. | fish are faced with a wide range of hydrostatic pressure (hp) in their natural habitats. additionally, freshwater fish are occasionally exposed to rapid changes in hp due to heavy rainfall, flood and/or dam release. accordingly, variations in hp are one of the most important environmental cues for fish. however, little information is available on how hp information is perceived and transmitted in the central nervous system of fish. the present study examined the effect of hp (water depth of 1.3 ... | 2015 | 25975379 |