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serotonergic synapses modulate generation of spikes from retinal ganglion cells of teleosts.serotonin [5-hydroxytryptamine (5-ht)] is a common neurotransmitter/neuromodulator found widely in the nervous system. cellular morphology and retinal distribution of serotonergic amacrine cells in the channel catfish (ictalurus punctatus) retina are identified using monoclonal anti-5ht antibody. these cells receive ribbon synapses from off-center (hyperpolarizing) bipolar cells as well as conventional synapses with other non-serotonergic amacrine cells. output synapses from the serotonergic cel ...200919938208
herbal supplementation diets on hematology and innate immunity in goldfish against aeromonas hydrophila.goldfish, carassius auratus (47 + or - 3 g, n = 300) were inoculated intramuscularly (50 microl) with aeromonas hydrophila (1.8 x 10(6) cells ml(-1)). on the 6th day of post-infection the fishes were divided into i) control, without infection fed with normal diet (c), ii) infected fish, fed with normal diet (iu), and infected fishes treated with different doses of iii) 100 mg kg(-1), iv) 200 mg kg(-1), iv) 400 mg kg(-1) and vi) 800 mg kg(-1) mixed herbal extracts supplementation diets. hematolog ...201019941960
methyltestosterone-induced changes in electro-olfactogram responses and courtship behaviors of cyprinids.in the tinfoil barb (barbonymus schwanenfeldii; family cyprinidae), we previously found that increased olfactory sensitivity to a female prostaglandin pheromone could induce sexual behavior display in juvenile fish treated with androgens. here, we determined if this phenomenon is widespread among cyprinid fishes by adding 17alpha-methyltestosterone (mt) to aquaria containing juveniles of 4 cyprinid species (tinfoil barbs; redtail sharkminnows, epalzeorhynchos bicolor; goldfish, carassius auratus ...201019965901
oxidative stress and apoptosis of carassius auratus lymphocytes induced by nonplanar (pcb153) and coplanar (pcb169) polychlorinated biphenyl congeners in vitro.among all the 209 kinds of polychlorinated biphenyls (pcbs) congeners, nonplanar and coplanar pcb congeners have different levels of toxicity on mammal cells such as neuronal cells, but little is known about their toxicity on fish cells although pcb congeners usually have high bioaccumulation abilities in the detected fish bodies. this study showed that 2,2',4,4',5,5'-hexacholorbiphenyl (pcb153, nonplanar congener) and 3,3',4,4',5,5'-hexacholorbiphenyl (pcb 169, coplanar congener) caused apoptos ...200919999978
physiological dimensions of ototoxic responses in a model fish species.pharmaceutical agents known to be toxic to the human auditory system also impair sensory hair cells of teleosts, and this supports the use of fish models for the screening of such compounds. however, previous investigations have focused almost exclusively on anatomical changes after drug administration without assessing macro-level physiological effects. using the goldfish (carassius auratus), we demonstrate that the acquisition of auditory evoked potentials offers a rapid and non-invasive means ...201020004583
prevention of ichthyophthirius multifiliis infestation in goldfish (carassius auratus) by potassium ferrate(vi) treatment.ichthyophthirius multifiliis is an important freshwater teleost pathogen that often leads to significant economic losses to the aquaculture industry. the purpose of this study was to assess the acute toxicity of potassium ferrate(vi) to i. multifiliis theront and the concentration needed to prevent i. multifiliis infestation in goldfish, carassius auratus. five hundred theronts were exposed to concentrations of potassium ferrate(vi) in each well of a 96-well microtiter plate and observed for 4h ...201020006444
genetic, biochemical, and individual responses of the teleost fish carassius auratus to uranium.carassius auratus were exposed for 96 h to different concentrations of uranyl nitrate (corresponding to 0, 100, 450, and 2,025 microg u l(-1)) and killed after different postexposure periods (0, 48, and 96 h) to assess uranium bioaccumulation, peroxisome proliferation (catalase [cat]), lipid peroxidation (thiobarbituric acid reactive substances [tbars]), and dna integrity in erythrocytes (comet assay). in addition, feeding behaviour was recorded as a general response to toxicant exposure. result ...201020012274
immune response and expression analysis of cathepsin k in goldfish during aeromonas hydrophila infection.the innate immunity and expression profiles of cathepsins d were determined in the goldfish (carassius auratus) tissues after challenge with a fish pathogen aeromonas hydrophila. the innate immunity of reactive oxygen species (ros) and reactive nitrogen species (rns) were determined by peripheral blood leucocytes. blood and tissue samples of the muscle, gills, liver, kidney, heart, spleen, and intestine were sampled at 1, 3, 6 and 12 h post-infection for cathepsin d expression by semi-quantitati ...201020025977
isolation, cloning, sequencing of brain type aromatase and its expression in male and female wrasse, pseudolabrus sieboldi.pseudolabrus sieboldi, wrasse being a diurnal spawner provides a good opportunity to study the endocrine mechanism of estrogen formation in brain and gonads. moreover, an extremely large amount of e2 was produced in serum and testis of wrasse. it is assumed that the presence of e2 may play a major role in diurnal gametogenesis in male fish. in this study brain type aromatase have been isolated, cloned and sequenced from the brain of wrasse. further, the expression pattern of brain type aromatase ...200520035447
olfactory sensitivity to bile fluid and bile salts in the european eel (anguilla anguilla), goldfish (carassius auratus) and mozambique tilapia (oreochromis mossambicus) suggests a 'broad range' sensitivity not confined to those produced by conspecifics alone.teleosts have high olfactory sensitivity to bile salts. to assess whether this phenomenon is involved in intra-specific chemical communication alone, or is part of a more ;broad range' sensitivity to bile salts produced by heterospecifics, we investigated possible differences in the odour of bile between the sexes and among different species - the eel (anguilla anguilla), goldfish (carassius auratus) and mozambique tilapia (oreochromis mossambicus) - using the electro-olfactogram (eog). we also ...201020038666
evaluation of dairy-yeast prebiotic supplementation in the diet of juvenile goldfish in the presence or absence of phytoplankton and zooplankton.prebiotics recently have been shown to increase immune responses and disease resistance in certain fish species; therefore, the current study was conducted to evaluate the commercially available dairy-yeast prebiotic, grobiotic-a, for use with juvenile goldfish carassius auratus. the study consisted of two 10-week feeding trials in which juvenile goldfish were fed practical diets that were either unsupplemented or supplemented with the dairy-yeast prebiotic at 2% by dry weight. juvenile fish wer ...200920043400
characterization of non-cytosolic hexokinase activity in white skeletal muscle from goldfish (carassius auratus l.) and the effect of cold acclimation.hk (hexokinase) is an enzyme involved in the first step in the glucose metabolism pathway, converting glucose into g6p (glucose 6-phosphate). owing to the importance of skeletal muscle for fish swimming and acclimation processes, we used goldfish (carassius auratus l.) white muscle in order to investigate subcellular distribution and kinetics of hk. in this study, we report that hk activity is predominantly localized in the mitochondrial fraction [nc-hk (non-cytosolic hk)] in goldfish white musc ...201020055755
biogeography and evolution of the carassius auratus-complex in east asia.carassius auratus is a primary freshwater fish with bisexual diploid and unisexual gynogenetic triploid lineages. it is distributed widely in eurasia and is especially common in east asia. although several genetic studies have been conducted on c. auratus, they have not provided clear phylogenetic and evolutionary descriptions of this fish, probably due to selection bias in sampling sites and the dna regions analysed. as the first step in clarifying the evolutionary entity of the world's carassi ...201020064277
susceptibility of koi x crucian carp and koi x goldfish hybrids to koi herpesvirus (khv) and the development of khv disease (khvd).hybrids of koi, cyprinus carpio x crucian carp, carassius carassius and koi x goldfish, carassius auratus, proved to be susceptible to koi herpesvirus (khv, syn. cyhv-3) and developed khv disease (khvd). while hybrids of koi x goldfish were partly resistant to mortality following infection by immersion, most koi x crucian carp hybrids died after bath infection. khv dna was detected in dead fish but also in all surviving animals by different polymerase chain reactions (pcrs). according to these r ...201020102462
toxic effects of crude-oil-contaminated soil in aquatic environment on carassius auratus and their hepatic antioxidant defense system.under the indoor simulant conditions, toxic effects of crude-oil-contaminated soil which was put into aquatic environment on the young fishes carassius auratus and their hepatic antioxidant system after a 20-d exposure were investigated. results showed that the relationship between the mortality of c. auratus and the exposed doses could be divided into 3 phases: fishes exposed to the low dose groups (0.5-5.0 g/l) were dead due to the ingestion of crude-oil-contaminated soils in aquatic environme ...200920108662
the zalpha domain of pkz from carassius auratus can bind to d(gc)(n) in negative supercoils.pkz was the most recently discovered member of eif2alpha kinase family in fish. capkz, the first identified fish pkz, possessed a conserved eif2alpha kinase catalytic domain in c-terminal and two z-dna binding domains (zalpha) in n-terminal. the zalpha of capkz closely resembled that of other z-dna binding proteins: adar1, dlm-1, and e3l. in order to understand more about the function of capkz, we expressed and purified three constructed peptides of capkz (p(zalpha)): p(zalpha1zalpha2), p(zalpha ...201020139004
effects of monocrotophos on the reproductive axis in the male goldfish (carassius auratus): potential mechanisms underlying vitellogenin induction.monocrotophos (mcp) is a highly toxic organophosphorus pesticide that has been banned in many countries. both vitellogenin mrna expression and secretion were significantly induced in male goldfish by exposure to an mcp-based pesticide, suggesting that mcp has significant estrogenic properties. to elucidate the mechanisms of action of mcp on vitellogenin induction, we used radioimmunoassay to examine the effect of mcp treatment on plasma 17beta-estradiol and testosterone levels in male goldfish ( ...201020149464
phytotherapy of ulcerative dermatitis induced by aeromonas hydrophila infection in goldfish ( carassius auratus ).goldfish ( carassius auratus ) weighing 13 +/- 2 g were administered intramuscularly a sublethal dose (1.8 x 10 3 cfu/ml) of aeromonas hydrophila to induce ulcerative dermatitis. on day 3 and day 6 after infection the fish were dip-treated (for 5 min/day) with a tri-herbal concoction of curcuma longa, ocimum sanctum and azadirachta indica (1%). the ld 50 value was recorded at a concentration of 2.3 x 10 4 between 30 and 36 days after infection. in the infected untreated group the cumulative mort ...201020159736
evolution of gustatory reflex systems in the brainstems of fishes.the great number of species of teleosts permits highly specialized forms to evolve to occupy particular niches. this diversity allows for extreme variations in brain structure according to particular sensory or motor adaptations. in the case of the taste system, goldfish (carassius auratus l., 1758) and some carps have evolved a specialized intraoral food-sorting apparatus along with corresponding specializations of gustatory centers in the brainstem. a comparison of circuitry within the complex ...200920160963
effect of chemotherapy, vaccines and immunostimulants on innate immunity of goldfish infected with aeromonas hydrophila.we report the effect of various chemicals, vaccines and immunostimulants on innate immune mechanisms such as phagocytosis activity, superoxide anion production of blood leukocytes, complement activity and plasma lysozyme activity and disease resistance in goldfish carassius auratus (23 +/- 2 g) against aeromonas hydrophila on days 15 and 30 post-infection. in infected fish, the administration of diets supplemented with either probiotics, tri-herbal extract or azadirachtin for 30 d significantly ...200920183964
pharmacokinetics of morphine after intramuscular injection in common goldfish carassius auratus and atlantic salmon salmo salar.teleost fish have a nociceptive system and likely perceive pain. this warrants the development of analgesic protocols both for experimental surgery and for various husbandry procedures. morphine is the standard analgesic against which the efficacy of other analgesics is assessed, and is the analgesic that has been most used in fish. the aims of this study were to describe the pharmacokinetics of morphine after an intramuscular (i.m.) injection in common goldfish and atlantic salmon, and to illus ...200920183965
active biomonitoring of complex pollution in taihu lake with carassius auratus.carassius auratus bred in clean water were selected to conduct active biomonitoring (abm) exposures in meiliang and gong bays in the northern polluted area of taihu lake. the biotransformation enzymes 7-ethoxyresorufin-o-deethylase (erod) and glutathione s-transferase (gst), antioxidant enzymes glutathione peroxidase (gp(x)), reduced glutathione (gsh) content and superoxide dismutase (sod) and lipoperoxidation (as tbars) in liver were determined as biomarkers during the field exposure period. at ...201020189215
in vivo anthelmintic activity of crude extracts of radix angelicae pubescentis, fructus bruceae, caulis spatholobi, semen aesculi, and semen pharbitidis against dactylogyrus intermedius (monogenea) in goldfish (carassius auratus).in search of a natural antiparasitic, in vivo anthelmintic activity of petroleum ether, chloroform, ethyl acetate, methanol, and aqueous extracts of angelica pubescens roots (radix angelicae pubescentis), brucea javanica fruits (fructus bruceae), spatholobus suberectus stems (caulis spatholobi), aesculus chinensis bge. seeds (semen aesculi), and pharbitis purpurea (l.) voigt seeds (semen pharbitidis) were tested against dactylogyrus intermedius (monogenea) in goldfish (carassius auratus). among ...201020191290
e-screen and vitellogenin assay for the detection of the estrogenic activity of alkylphenols and trace elements.the estrogenic potential of 4-nonylphenol (4-np), 4-octylphenol (4-op), p-t-octylphenol (p-t-op) and three trace elements, lead (pb), copper (cu) and cadmium (cd(no(3))(2) and cdcl(2)), were compared in two different tests, a proliferation assay with estrogen receptor-positive human mcf-7 breast cancer cells (e-screen) and the induction of vitellogenin (vtg) in juvenile goldfish (carassius auratus). the results showed differences in the bioassays' sensitivity and potency with the following order ...201020197112
inhibition of goldfish mitochondrial metabolism by in vitro exposure to cd, cu and ni.although impairment of aerobic capacities has been reported in metal-contaminated wild fish, little is known about the direct toxicity of the metals themselves at the low concentrations found in the field compared to indirect consequences mediated by metal effects on ecological variables such as prey type and abundance, predation and competition. this study examined the in vitro effects of cd, cu and ni on mitochondrial enzyme activity and maximal (state 3) mitochondrial oxygen consumption rate ...201020207426
evidence for a distributed respiratory rhythm generating network in the goldfish (carsssius auratus).central pattern generators located in the brainstem regulate ventilatory behaviors in vertebrates. the development of the isolated brainstem preparation has allowed these neural networks to be characterized in a number of aquatic species. the aim of this study was to explore the architecture of the respiratory rhythm-generating site in the goldfish (carassius auratus) and to determine the utility of a newly developed isolated brainstem preparation, the sheep dip. here we provide evidence for a d ...201020217310
[epidemiological survey on clonorchiasis sinensis in an endemic area of south hunan province].to make an epidemiological investigation on clonorchiasis sinensis and relevant factors in the south of hunan province.200920232625
influence of temperature on the asphyxiation of young goldfish (carassius auratus l.) under various tensions of oxygen and carbon dioxide. 194720249526
effects of monocrotophos on the reproductive axis in the female goldfish (carassius auratus).monocrotophos (mcp) is a highly toxic organophosphorus pesticide. to elucidate the influence of mcp on female fish reproduction, plasma 17beta-estradiol, testosterone and gonadotropin levels and aromatase and gonadotropin beta subunit gene expression levels were examined in female goldfish (carassius auratus) following a 21-day exposure to 0.01, 0.10 and 1.00 mg l-(1) 40% mcp-based pesticide in a semi-static exposure system. the results indicated that mcp induced increases in plasma 17beta-estra ...201020298809
chromium-induced biochemical, genotoxic and histopathologic effects in liver and kidney of goldfish, carassius auratus.fish constitute an excellent model to understand the mechanistic aspects of metal toxicity vis-à-vis oxidative stress in aquatic ecosystems. hexavalent chromium (cr (vi)), due to its redox potential can induce oxidative stress (os) in fish and impair their health. in the present investigation, we hypothesize that os plays a key role in chromium induced toxicity in goldfish; leading to the production of reactive oxygen species (ros) such as o(.)(2), h(2)o(2), oh(.), and subsequent modulation of t ...201020348018
some additional data to the occurrence, morphology and validity of myxobolus turpisrotundus zhang, 2009 (myxozoa: myxosporea).myxobolus turpisrotundus zhang, 2009, infects allogynogenetic gibel carp carassius auratus gibelio (bloch) and is always regarded as synonymous with myxobolus rotundus nemeczek, 1911, since its first report in goldfish carassius auratus auratus (l.) in china in 1955. in this study, it was comprehensively examined by morphological and molecular biological methods. the round spores of m. turpisrotundus are similar to those of m. rotundus from common bream abramis brama (l.) in morphology; however, ...201020352450
[changes in protein composition in the goldfish tissues depending on season and temperature of keeping].data of sds-electrophoresis of tissues proteins of the goldfish carassius auratus netted in winter and summer and kept at different temperatures (+5 degrees c and +20 degrees c) are presented. it has been ascertained that after keeping the "winter" fish at 5 degrees c for 2 weeks quantities of the protein 205 kd in the red muscles and heart and the protein 172 kd in the liver are significantly decreased. a slight decline in contents of the proteins 105 and 45 kd was observed in the white muscles ...201520387652
oxygen consumption and ammonia excretion of black carp (mylopharyngdon piceus richardson) and allogynogenetic crucian carp (carassius auratus gibelio female x cyprinus carpio male) fed different carbohydrate diets.oxygen consumption and ammonia excretion of black carp (mylopharyngdon piceus richardson) (4.6±0.3 g) and allogynogenetic crucian carp (carassius auratus gibelio ♀×cyprinus carpio ♂) (5.7±0.5 g) were examined when fish fed two types of carbohydrate (dextrin and glucose) at two levels (20 and 40%) each. the diets were isonitrogenous (40% dry matter) and isocaloric at 18.5 kj g(−1) (dry matter) by adjusting the oil content to 10.1 and 1.5%, respectively. in black carp, the interactions between the ...201020390347
frequency tuning and intensity coding of sound in the auditory periphery of the lake sturgeon, acipenser fulvescens.acipenser fulvescens, the lake sturgeon, belongs to one of the few extant non-teleost ray-finned (bony) fishes. the sturgeons (family acipenseridae) have a phylogenetic history that dates back about 250 million years. the study reported here is the first investigation of peripheral coding strategies for spectral analysis in the auditory system in a non-teleost bony fish. we used a shaker system to simulate the particle motion component of sound during electrophysiological recordings of isolated ...201020400642
in vivo anthelmintic activity of five alkaloids from macleaya microcarpa (maxim) fedde against dactylogyrus intermedius in carassius auratus.the present study aims to evaluate the anthelmintic properties of aerial part of macleaya microcarpa (maxim) fedde. bioassay-guided fractionation and isolation of the compounds with anthelmintic activity were performed on the ethanolic extract of m. microcarpa yielding five bioactive alkaloids namely: sanguinarine, cryptopine, beta-allocryptopine, protopine and 6-methoxyl-dihydro-chelerythrine by comparing spectral data (uv, nmr, and ei-ms) with literature values. according to in vivo anthelmint ...201020413222
reactions of goldfish (carassius auratus) to three suture patterns following full thickness skin incisions.three different suture patterns (simple interrupted, interrupted horizontal mattress, subcuticular) were placed in a full thickness incision (skin and body wall) of 18 goldfish (carassius auratus). after 14 days all fish were euthanized using benzocaine solution. the tissue reactions were evaluated by gross visual inspection and histopathological examination. the superficial inflammatory reactions were graded on a scale from 0 (no inflammation) to 3 (severe inflammation). the inflammatory respon ...201020434740
induction of virus-specific cell-mediated cytotoxic responses of isogeneic ginbuna crucian carp, after oral immunization with inactivated virus.clonal ginbuna crucian carp (carassius auratus langsdorfii) were immunized orally with inactivated crucian carp hematopoietic necrosis virus (chnv) and the cytotoxic activities of peripheral blood leukocytes against chnv-infected syngeneic target cells were investigated. although a single oral administration of the vaccine did not prime measurable cytotoxic responses to chnv-infected targets, detectable lytic activities were observed after a booster oral administration. the vaccine-induced cytot ...201020438852
studies on the developmental potentiality of cultured cell nuclei of fish.by means of the serial nuclear transplantation technique, the authors obtained a nuclear transplant fish from subcultured cell originated from the blastula cells of the crucian carp (carassius auratus linnaeus). this nuclear transplant fish survived for three years, but its sexual glands were undifferentiated. the authors have also obtained a sexually mature adult fish from short-term cultured kidney cell nucleus of an adult crucian carp. results of the experiment implied that the subcultured ce ...201020440385
aeromonas hydrophila pepo outer membrane endopeptidase activates human big endothelin-3 in vitro and induces skin ulcer in goldfish (carassius auratus).in aeromonas hydrophila, the gram-negative bacterial fish pathogen, pepo constitutes the thermoregulated outer membrane m13 family zinc endopeptidase, which is expressed maximally at 16 degrees c and is down-regulated above 30 degrees c. cultivation of a. hydrophila at 16 degrees c enabled it to activate big endothelin (et), the vasoconstrictor and ulcerogenic peptide naturally secreted from human vascular endothelial cell (huvec) culture. furthermore, a. hydrophila pepo in vitro shows strong en ...201020456877
[genetic homeostasis and developmental stability in natural populations of two-gender (carassius auratus) and one-gender (c. gibelio) goldfishes].comparative analysis of one amphymictic diploid carassius auratus, three apomictic triploid c. gibelio and hybrid triploid carassius auratus-gibelio forms of gold fish was performed at the complex of characteristics of developmental stability: morphological variation, fluctuating asymmetry and phenodeviations. it was found that c. gibelio forms are characterized by lower levels both of morphological variation and fluctuating asymmetry and more rare cases of morphological abnormalities. a hybrid ...201020458966
integrated biomarker response index for the assessment of environmental stress of the yangtze river (nanjing section).in the present study, multibiomarker effects of the river water of three representative sections in nanjing section of the yangtze river were investigated in goldfish (carassius auratus). the organic toxicants were extracted from the water samples using solid phase extraction. acetylcholinesterase (ache), glutathione-s-transferase (gst), 7-ethoxyresorufin-o-deethylase (erod), glutathione peroxidase (gpx) and na+/k+-atpase activities were determined after exposure of the extracted components. the ...201020473565
[influence of copper ions on hematological and cytogenetic parameters of fresh-water fishes carassius auratus gibelio].influence of copper ions on white blood cells parameters in carassius auratus gibelio was investigated. changes in cytogenetic parameters and instability in erythrocytes and epithelial cells of gills were estimated in reply to influence of copper solutions. the frequency of epithelial gill cells at a stage of apoptosis was investigated. the opportunity to use the hematological parameters and cytogenetic instability set for xenobiotics effects in fishes was shown.201320480813
molecular cloning and effect of c-fos mrna on pharmacological stimuli in the goldfish brain.c-fos is an immediate early gene, and is rapidly and transiently induced in neurons of the central nervous system according to their activities. to investigate neuronal activities in the brain of the goldfish (carassius auratus), we considered that expression of c-fos mrna would be an available marker for the neuronal activities. therefore, we firstly isolated a cdna clone encoding c-fos from the goldfish brain by rt-pcr and race methods. a full length cdna of the goldfish c-fos was composed of ...200620483256
infection of myxobolus turpisrotundus sp. n. in allogynogenetic gibel carp, carassius auratus gibelio (bloch), with revision of myxobolus rotundus (s. l.) nemeczek reported from c. auratus auratus (l.).infection of a myxobolus species, previously identified as myxobolus rotundus, was detected in 182 of 7892 (2.31%) allogynogenetic gibel carp, carassius auratus gibelio, in a closed pond culture system in china. morphological and molecular data showed that this myxosporean is a different species from m. rotundus parasitizing abramis brama in europe and is thus designated as a new species, myxobolus turpisrotundus. m. rotundus (s.l.) ex c. auratusauratus is a synonym of m. turpisrotundus. plasmod ...201020487139
hormonal and ion regulatory response in three freshwater fish species following waterborne copper exposure.we evaluated effects of sublethal copper exposure in 3 different freshwater fish: rainbow trout (oncorhynchus mykiss), common carp (cyprinus carpio) and gibel carp (carassius auratus gibelio). in a first experiment we exposed these fishes to an equally toxic cu dose, a cu level 10 times lower than their 96 h lc50 value: 20, 65, and 150 microg/l cu. in a second series we exposed them to the same cu concentration (50 microg/l). na+/k+-atpase activity in gill tissue was disturbed differently in rai ...201020488257
argulus japonicus: sperm transfer by means of a spermatophore on carassius auratus (l).the process of sperm transfer is somewhat enigmatic in argulus, even though copulation has been witnessed. a breeding colony of argulus japonicus was kept under laboratory conditions in order to study reproduction in the species. pairs in copula were removed and studied with histology and scanning electron microscopy to describe the mechanism of sperm transfer. sections of copulating pairs revealed sperm on the accessory copulatory structures of the male's swimming legs; and scanning electron mi ...201020493846
kinetics of cd4+ and cd8alpha+ t-cell subsets in graft-versus-host reaction (gvhr) in ginbuna crucian carp carassius auratus langsdorfii.we have previously demonstrated the presence of graft-versus-host reaction (gvhr) in fish employing a model system of clonal triploid ginbuna and tetraploid ginbuna-goldfish hybrids. to elucidate the role of cd8alpha+ t cells in the induction of gvhr, we investigate the kinetics of cd4+ and cd8+ t-cell subsets in gvhr along with the pathological changes associated with gvh disease (gvhd) in ginbuna. gvhr was not induced with a leukocyte fraction lacking cd8alpha+ t cells separated by magnetic ce ...201020493902
a set of polymorphic trinucleotide and tetranucleotide microsatellite markers for silver crucian carp (carassius auratus gibelio) and cross-amplification in crucian carp.silver crucian carp (carassius auratus gibelio bloch), as a gynogenetic fish, is a promising model for the study of the evolutionary genetics of vertebrates. we have developed 59 polymorphic trinucleotide and tetranucleotide markers for the silver crucian carp through the biotin capture method and radioactive-labeling hybridization. the number of alleles ranged from 2 to 12 in the population, and the average proportion of heterozygotes (including tri- and diallelic) at polymorphic loci was 76.8% ...201020503069
comparison of macrophage antimicrobial responses induced by type ii interferons of the goldfish (carassius auratus l.).unlike mammals, bony fish have two type ii interferons, ifngamma and ifngammarel, whose pro-inflammatory functions have not been fully characterized. to elucidate the distinct roles of these type ii interferons of bony fish, we examined the effects of recombinant goldfish (rg) ifngamma and ifngammarel on the macrophage antimicrobial responses, immune gene expression, and their signaling pathways. our findings indicate that rgifngamma and rgifngammarel possess unique capacities to mediate each of ...201020507977
myxobolus turpisrotundus (myxosporea: bivalvulida) spores with caudal appendages: investigating the validity of the genus henneguya with morphological and molecular evidence.spores of the myxozoan parasite myxobolus turpisrotundus zhang 2009 were observed for the first time bearing caudal appendages. most spores had the typical myxobolus spp. morphology, but approximately 10% of spores possessed a spore body that was slightly elongated with a short tail projecting from the spore valve. in other spores, the tail was much more clearly visible and elongate. the spore body of these unusual spores is consistent in morphology and dimension to the normal spores of m. turpi ...201020512504
effect of intraperitoneal injection of curcumin on food intake in a goldfish model.although spice compounds have several pharmacological and biochemical actions such as antioxidant activity, their physiological effects on neuropeptides related to feeding regulation are not well known. the aim of the present study was to identify the pharmacological activities of spice compounds on appetite regulation using a goldfish (carassius auratus) model with emphasis on the role of neuropeptides. the spice compounds used in this study were curcumin, piperine, and ursolic acid. goldfish w ...201120514526
optimization of somatic cell injection in the perspective of nuclear transfer in goldfish.nuclear transfer has the potential to become one strategy for fish genetic resources management, by allowing fish reconstruction from cryopreserved somatic cells. survival rates after nuclear transfer are still low however. the part played by unsuitable handling conditions is often questioned, but the different steps in the procedure are difficult to address separately. in this work led on goldfish (carassius auratus), the step of somatic cells injection was explored. non-enucleated metaphase ii ...201020529309
effects of montmorillonite on alleviating dietary cd-induced oxidative damage in carp (carassius auratus).the present study was designed to investigate the effects of montmorillonite (mmt) on dietary cd-induced oxidative damage in liver and kidney of carp (carassius auratus). one hundred eighty carp were randomly divided into four groups and fed with a basal diet, a basal diet supplemented with 0.5% mmt, cd-comtaminated basal diet (120 mg cd/kg dry weight) and cd-contaminated basal diet supplemented with 0.5% mmt, respectively. after 60 days, fish were sacrificed to measure malondialdehyde (mda) con ...201120532670
the pattern of expression of guanine nucleotide-binding protein beta3 in the retina is conserved across vertebrate species.guanine nucleotide-binding protein beta3 (gnb3) is an isoform of the beta subunit of the heterotrimeric g protein second messenger complex that is commonly associated with transmembrane receptors. the presence of gnb3 in photoreceptors, and possibly bipolar cells, has been confirmed in murine, bovine and primate retinas [lee rh, lieberman bs, yamane hk, bok d, fung bk (1992) j biol chem 267:24776-24781; peng yw, robishaw jd, levine ma, yau kw (1992) proc natl acad sci u s a 89:10882-10886; huang ...201020538044
chirping response of weakly electric knife fish (apteronotus leptorhynchus) to low-frequency electric signals and to heterospecific electric fish.brown ghost knife fish (apteronotus leptorhynchus) can briefly increase their electric organ discharge (eod) frequency to produce electrocommunication signals termed chirps. the chirp rate increases when fish are presented with conspecific fish or high-frequency (700-1100 hz) electric signals that mimic conspecific fish. we examined whether a. leptorhynchus also chirps in response to artificial low-frequency electric signals and to heterospecific electric fish whose eod contains low-frequency co ...201020543122
cytotoxicity of chromium ions may be connected with induction of oxidative stress.chromium ions are frequently found in aquatic ecosystems and are known to be inducers of oxidative stress in fish solid tissues. the present study was designed to determine whether fish blood samples can be used to allow nonlethal diagnostic testing for chromium intoxication. first, we confirmed that 96 h exposures to water containing 10.0 mg l(-1) chromium ions, either cr3+ or cr6+, induced oxidative stress in brain of goldfish (carassius auratus). multiple blood parameters were then evaluated. ...201020547405
hexavalent chromium-induced multiple biomarker responses in liver and kidney of goldfish, carassius auratus.hexavalent chromium [cr (vi)] is a constituent of chromite ore. although it is known to have several industrial and technological applications, its release into the aquatic environment as a result of chemical spill or inadequate waste discharge may hamper the health of aquatic organisms. in this study, we have investigated the effects of cr (vi) on multiple biomarkers responses in goldfish under subchronic exposure conditions. laboratory-acclimatized fish were exposed to 4.25 ppm and 8.57 ppm cr ...201020549632
responses of ache and gst activities to insecticide coexposure in carassius auratus.organophosphates and carbamates are widely used pesticides and play an important role in global agriculture. the misuse of these compounds has caused environmental problems and has had a negative impact on wildlife. in this study, the in vivo effects of commercial chlorpyrifos and isoprocarb on acetylcholinesterase (ache) and glutathione s-transferase (gst) activities in goldfish (carassius auratus) were investigated. muscle and brain ache activity was significantly inhibited by chlorpyrifos and ...201220549641
two functional growth hormone secretagogue receptor (ghrelin receptor) type 1a and 2a in goldfish, carassius auratus.here we report the identification and characterization of ghrelin (grln) receptors in goldfish carassius auratus. we identified four distinct mrnas generated from four different genes. those were roughly divided into two types, based on the number of amino acids and amino acid sequence similarity; one composed of 360-amino acids, which is similar to zebrafish ghs-r1a (showing 94-96% identity) and the other encodes a 366- or 367-amino acid protein, which demonstrated 95% identity to zebrafish ghs ...201020558240
genetic basis and breeding application of clonal diversity and dual reproduction modes in polyploid carassius auratus gibelio.a unisexual species is generally associated with polyploidy, and reproduced by a unisexual reproduction mode, such as gynogenesis, hybridogenesis or parthenogenesis. compared with other unisexual and polyploid species, gibel carp (carassius auratus gibelio) has a higher ploidy level of hexaploid. it has undergone several successive rounds of genome polyploidy, and experienced an additional, more recent genome duplication event. more significantly, the dual reproduction modes, including gynogenes ...201020596906
distant hybridization leads to different ploidy fishes.distant hybridization makes it possible to transfer the genome of one species to another, which results in changes in phenotypes and genotypes of the progenies. this study shows that distant hybridization or the combination of this method with gynogenesis or androgenesis lead to different ploidy fishes with genetic variation, including fertile tetraploid hybrids, sterile triploid hybrids, fertile diploid hybrids, fertile diploid gynogenetic fish, and their derived progenies. the formations of th ...201020596907
a molecule in teleost fish, related with human mhc-encoded g6f, has a cytoplasmic tail with itam and marks the surface of thrombocytes and in some fishes also of erythrocytes.in teleost fish, a novel gene g6f-like was identified, encoding a type i transmembrane molecule with four extracellular ig-like domains and a cytoplasmic tail with putative tyrosine phosphorylation motifs including yxn and an immunoreceptor tyrosine-based activation motif (itam). g6f-like maps to a teleost genomic region where stretches corresponding to human chromosomes 6p (with the mhc), 12p (with cd4 and lag-3), and 19q are tightly linked. this genomic organization resembles the ancestral "ur ...201020614118
fish virus-induced interferon exerts antiviral function through stat1 pathway.virus-induced interferons (ifns) have been identified in various fish species and display antiviral activities similar to mammalian type i ifns. however, apart from the mammalian ifn system, the ifn signaling pathway remains largely unknown. using transient transfection and recombinant protein, we are reporting in this study that a crucian carp (carassius auratus l.) ifn exhibits strong antiviral activity against grass carp hemorrhagic virus (gchv) infection and also mediates poly i:c-induced an ...201020627394
assessment of in vivo estrogenic response and the identification of environmental estrogens in the yangtze river (nanjing section).environmental estrogens in the aquatic environment have been shown to be responsible for the feminization of fish. the estrogenic content of the yangtze river (nanjing section--referred to as the studied area herein) was assessed using a combination of bioassay and chemical analysis. the in vivo bioassay was conducted by exposing adult male goldfish (carassius auratus) to different concentrations of river water (25%, 50% and 100%) sampled from three representative sections of the studied area. c ...201020646735
melatonin-synthesizing enzymes in pineal, retina, liver, and gut of the goldfish (carassius): mrna expression pattern and regulation of daily rhythms by lighting conditions.it has been suggested that melatonin is synthesized in nonphotosensitive organs of vertebrates in addition to the well-known sites of the pineal gland and retina. however, very few studies have demonstrated the gene expression of melatonin-synthesizing enzymes in extrapineal and extraretinal locations. this study focuses on the circadian expression of the two key enzymes of the melatoninergic pathway, arylalkylamine n-acetyltransferase (aanat) and hydroxyindole-o-methyltransferase (hiomt), in ce ...201020653449
roles of nitric oxide, nitrite and myoglobin on myocardial efficiency in trout (oncorhynchus mykiss) and goldfish (carassius auratus): implications for hypoxia tolerance.the roles of nitric oxide synthase activity (nos), nitrite and myoglobin (mb) in the regulation of myocardial function during hypoxia were examined in trout and goldfish, a hypoxia-intolerant and hypoxia-tolerant species, respectively. we measured the effect of nos inhibition, adrenaline and nitrite on the o(2) consumption rate and isometric twitch force development in electrically paced ventricular preparations during hypoxia, and measured o(2) affinity and nitrite reductase activity of the pur ...201020675545
copper toxicity in gibel carp carassius auratus gibelio: importance of sodium and glycogen.in general, copper is primarily an osmoregulatory toxicant to fish and cu toxicity is thought to be related to the rate of sodium loss. looking at a previous research it is striking that gibel carp, carassius auratus gibelio, do not seem so susceptible to the first ionoregulatory shock phase of cu exposure, but rather build up physiological disturbances slowly until mortality occurs. since it was noted that gibel carp experience severe hypoxia under cu exposure, we hypothesised that, besides the ...201020684062
[comparative characteristic of lactate dehydrogenase properties from the white muscles and liver of goldfish (carassius auratus l.)].lactate dehydrogenase (ldh) from the liver and white muscles of the gold fish carassius auratus was partially purified by differential precipitation with ammonium sulfate. the enzyme from the liver had relatively low vmax--1.85 +/- 0.31 u/mg protein, the muscle enzyme--3.74 +/- 0.27 u/mg protein. ldh from the liver was less sensitive to substrate inhibition (i50 - 9.92 +/- 1.09 mm) compared to white muscles isozyme (i50 - 5.87 +/- 1.39 mm). the studied isozymes had ph-dependencies with ph optima ...201520684242
the anorexigenic effect of cholecystokinin octapeptide in a goldfish model is mediated by the vagal afferent and subsequently through the melanocortin- and corticotropin-releasing hormone-signaling pathways.we have been extensively investigating the mechanisms by which neuropeptides regulate feeding behavior by using a goldfish (carassius auratus) model. in this species, the anorexigenic action of melanocortin peptide is centrally mediated via the corticotropin-releasing hormone (crh)/crh receptor neuronal system, whereas sulfated cholecystokinin octapeptide (cck-8s) is involved in the appetite regulation as a peripheral anorexigenic factor. the aim of the present study was to identify the mechanis ...201020688118
anthelmintic activity of steroidal saponins from dioscorea zingiberensis c. h. wright against dactylogyrus intermedius (monogenea) in goldfish (carassius auratus).dactylogyrus intermedius is one of the most pathogenic monogenean parasites on the gills of captive fish and can cause serious problem in aquaculture. to attempt controlling this parasite and explore novel potential antiparasitic agents, the present study was designed to investigate the anthelmintic activity of dioscorea zingiberensis c. h. wright against d. intermedius in goldfish under in vivo conditions. bioactivity-guided fractionation and isolation of the compounds responsible for anthelmin ...201020689967
waterborne fluoxetine disrupts feeding and energy metabolism in the goldfish carassius auratus.fluoxetine (flx) is one of the most commonly detected pharmaceuticals in wastewater and bioaccumulates in wild-caught fish, especially in brain, liver and muscle tissues. previous studies indicated that flx is pharmacologically active in fish species exerting anorexigenic effects, but it is not clear whether waterborne flx has any potential effects on regulating food intake and energy metabolism. in this study, we investigated the effect of two doses of flx, an environmental concentration of 540 ...201020692053
speciation of polyploid cyprinidae fish of common carp, crucian carp, and silver crucian carp derived from duplicated hox genes.recent studies on comparative genomics have suggested that a round of fish-specific whole genome duplication (3r) in ray-finned fishes might have occurred around 226-316 mya. additional genome duplication, specifically in cyprinids, may have occurred more recently after the divergence of the teleosts. the timing of this event, however, is unknown. to address this question, we sequenced four hox genes from taxa representing the polyploid cyprinidae fish, common carp (cyprinus carpio, 2n=100), cru ...201020700889
biological traits of rare males in the population of carassius gibelio (actinopterygii: cyprinidae) from lake pamvotis (north-west greece).the gynogenetic population of prussian carp carassius gibelio in lake pamvotis (north-west greece) included a small percentage of males (2-3%). cytogenetic analysis revealed heterogeneity of the population at the ploidy level: all females were triploid (modal chromosome number 156-162 chromosomes) while the males were either triploid (modal number 156-158 chromosomes) or tetraploid (modal number 200-214 chromosomes). morphometric analysis revealed significant differences in body shape among thes ...201020701641
spatial learning-induced egr-1 expression in telencephalon of gold fish carassius auratus.the immediate-early gene (egr-1) expression was used to examine the neuron's response in telencephalon of goldfish during spatial learning in small space. fishes were pre-exposed in the experimental apparatus and trained to pick food from the tray in a rectangular-shaped arena. the apparatus was divided into identical compartments comprising three gates to provide different spatial tasks. after the fish learned to pass through the gate one, two more gates were introduced one by one. fish made mo ...201120714804
transmission of cyprinid herpesvirus-3 (cyhv-3) from goldfish to naïve common carp by cohabitation.cyprinid herpesvirus-3 (cyhv-3) has spread worldwide and has had a major impact on koi and common carp production. previous studies on the host range of the cyhv-3 found that fish species other than koi and common carp are fully resistant to natural virus exposure. recently, cyhv-3 was detected in goldfish (carassius auratus auratus) that were in contact with cyhv-3 infected koi. in the present study, a specific rt-pcr product was amplified from the viral thymidine kinase gene in gills, intestin ...201120723951
no evidence of uv cone input to mono- and biphasic horizontal cells in the goldfish retina.many animal species make use of ultraviolet (uv) light in a number of behaviors, such as feeding and mating. the goldfish (carassius auratus) is among those with a uv photoreceptor and pronounced uv sensitivity. little is known, however, about the retinal processing of this input. we addressed this issue by recording intracellularly from second-order neurons in the adult goldfish retina. in order to test whether cone-driven horizontal cells (hcs) receive uv cone inputs, we performed chromatic ad ...201020734051
[genetic structure of silver crussian carp carassius (superspecies auratus) (linnaeus, 1758) colonies from the middle dnieper basin].population genetic structure of silver prussian carp from the middle dnieper basin was established by means of biochemical gene marking and cytometric analysis. in addition to diploid goldfish carassius auratus, which was the basic species of bisexual colonies, a number of biotypes of triploid silver prussian carp c. gibelio were identified. these biotypes either formed isolated unisexual populations, or were an admixture to the bisexual colonies. in addition, in bisexual colonies, triploid fema ...201020734774
swimming in four goldfish carassius auratus morphotypes: understanding functional design and performance employing artificially selected forms.four goldfish carassius auratus morphotypes of similar length (50 mm): common (streamlined, full complement of paired and median fins, bifurcated caudal fin), comet ('common like' but with a long, deeply forked caudal fin), fantail (short, deep body with twinned caudal and anal fins) and eggfish (similar to the fantail but lacking a dorsal fin) were compared. drag, steady swimming kinematics, energetics, fast-start performance, stability in yaw and roll and propulsive muscle ultrastructural char ...200920738559
modulation of rhodopsin gene expression and signaling mechanisms evoked by endothelins in goldfish and murine pigment cell lines.endothelins (ets) and sarafotoxins (srtxs) belong to a family of vasoconstrictor peptides, which regulate pigment migration and/or production in vertebrate pigment cells. the teleost carassius auratus erythrophoroma cell line, gem-81, and mus musculus b16 melanocytes express rhodopsin, as well as the et receptors, etb and eta, respectively. both cell lines are photoresponsive, and respond to light with a decreased proliferation rate. for b16, the doubling time of cells kept in 14-h light (14l):1 ...201020802974
differential ablation of sensory receptors underlies ototoxin-induced shifts in auditory thresholds of the goldfish (carassius auratus).in recent years, fish models have become popular for investigations of ototoxic agents. however, the vast majority of such studies have focused on anatomical changes in lateral line hair cells after drug administration. using the goldfish (carassius auratus), we confirm that the acquisition of auditory evoked potentials offers a rapid and non-invasive method for quantifying ototoxin-induced changes in hearing sensitivity. gentamicin (100 mg ml(-1)) was the drug of choice as it is a well-studied ...201020809542
perforin-dependent cytotoxic mechanism in killing by cd8 positive t cells in ginbuna crucian carp, carassius auratus langsdorfii.t cell-mediated cytotoxicity occurs via pathways based on perforin or fas mechanisms. perforin is a protein present in the cytoplasmic granules of cd8(+) cytotoxic t lymphocytes and is secreted to form pores on target cell membranes. in fish, although the involvement of perforin in cytotoxicity have been suggested for several species, perforin-mediated cytotoxicity of cd8α(+) lymphocyte in conjunction with expression of the perforin gene has not been reported. in order to investigate the killing ...201120813128
bilateral intraocular malignant neuroectodermal tumors in a telescope goldfish (carassius auratus).abstract a 5-year-old male telescope goldfish (carassius auratus) developed buphthalmia of the left eye. an enucleation was performed and a diagnosis of a neuroectodermal tumor was made on histological examination. although the fish initially recovered, it was killed 49 days postsurgery due to a severe decline in its condition. on histological evaluation of postmortem tissue samples, it was determined that the fish also had a neuroectodermal tumor of the right eye with local invasion of the brai ...201020840084
waterborne fluoxetine disrupts the reproductive axis in sexually mature male goldfish, carassius auratus.fluoxetine (flx) is a pharmaceutical acting as a selective serotonin reuptake inhibitor and is used to treat depression in humans. fluoxetine and the major active metabolite norfluoxetine (nflx) are released to aquatic systems via sewage-treatment effluents. they have been found to bioconcentrate in wild fish, raising concerns over potential endocrine disrupting effects. the objective of this study was to determine effects of waterborne flx, including environmental concentrations, on the reprodu ...201020864192
anthelmintic activity of the crude extracts, fractions, and osthole from radix angelicae pubescentis against dactylogyrus intermedius in goldfish (carassius auratus) in vivo.a bioassay-guided fractionation was performed to evaluate the anthelmintic activity of the crude extract fractions and osthole from radix angelicae pubescentis against dactylogyrus intermedius in goldfish (carassius auratus) in vivo. among four extracts (petroleum ether, ethyl acetate, acetone, and ethanol), only ethanol extract exhibited the best anthelmintic efficacy with 100% mortality of dactylogyrus and no death of fish at the optimal anthelminthic concentration of 120 mg/l. therefore, etha ...201020878184
dynamic distribution of spindlin in nucleoli, nucleoplasm and spindle from primary oocytes to mature eggs and its critical function for oocyte-to-embryo transition in gibel carp.spindlin (spin) was thought as a maternal-effect factor associated with meiotic spindle. its role for the oocyte-to-embryo transition was suggested in mouse, but its direct evidence for the function had been not obtained in other vertebrates. in this study, we used the cagspin-specific antibody to investigate cagspin expression pattern and distribution during oogenesis of gibel carp (carassius auratus gibelio). first, the oocyte-specific expression pattern and dynamic distribution was revealed i ...201020878746
respiratory responses to hypoxia or hypercapnia in goldfish (carassius auratus) experiencing gill remodelling.the presence of an interlamellar cell mass (ilcm) on the gills of goldfish significantly decreases the functional lamellar surface area and increases the diffusion distance for gas transfer and thus may impose a serious challenge for the transfer of respiratory gases (o₂ and co₂). here we tested the hypothesis that the presence of the ilcm in goldfish acclimated to 7°c impedes the uptake of o2 and excretion of co₂. while pa(o₂) remained unaltered, the baseline values of pa(co)₂ were significantl ...201120934539
serotonin-induced contraction in isolated intestine from a teleost fish (carassius auratus): characterization and interactions with melatonin.serotonin (5-ht) plays a critical role in several gastrointestinal functions in vertebrates. in teleosts lacking enterochromaffin cells, intestinal 5-ht originates from serotonergic enteric neurons. in the present study, the foregut of a stomachless teleost, the goldfish (carassius auratus), was used to evaluate the in vitro effect of 5-ht on fish intestinal motility. we also studied the role of melatonin (mel), an indoleamine sharing the biosynthetic pathway with 5-ht, as regulator of serotoner ...201020939846
immunohistochemical and immunochemical characterization of the distribution of leptin-like proteins in the gastroenteric tract of two teleosts (dicentrarchus labrax and carassius auratus l.) with different feeding habits.leptin is a modulator of food intake and energy homeostasis both in mammals and in some species of nonmammals vertebrates. in this study, we reported for the first time, using an immunohistochemical and immunochemical approach, the presence and distribution of immunoreactivity to leptin-like protein in the gastroenteric tract of dicentrarchus labrax (bass) and carassius auratus (goldfish), two teleostean species with different feeding and different adaptative morphological organization of the ga ...201120945462
the consequences of reversible gill remodelling on ammonia excretion in goldfish (carassius auratus).goldfish acclimated to cold water (e.g. 7°c) experience a marked reduction in functional lamellar surface area owing to the proliferation of an interlamellar cell mass (ilcm), a phenomenon termed gill remodelling. the goal of the present study was to assess the consequences of the reduced functional surface area on the capacity of goldfish to excrete ammonia. despite the expected impact of ambient temperature on functional surface area, fish acclimated to 7°c and 25°c exhibited similar rates of ...201020952613
the control of breathing in goldfish (carassius auratus) experiencing thermally induced gill remodelling.at temperatures below 15°c the gill lamellae of goldfish (carassius auratus) are largely covered by an interlamellar cell mass (ilcm) which decreases the functional surface area of the gill. the presence of the ilcm in goldfish acclimated to cold water conceivably could lead to a covering of the neuroepithelial cells (necs), which are believed to be important for sensing ambient o₂ and co₂ levels. in this study we tested the hypothesis that goldfish with covered lamellae (and presumably fewer ne ...201020952614
impact of a secondary treated bleached kraft pulp mill effluent in both sexes of goldfish (carassius auratus l.).the aim of the present study was to assess the toxic effects of a secondary treated effluent from a modern pulp mill processing eucalyptus globulus on both sexes of goldfish. the effects in the exposed fish were investigated by measuring biomarkers which have been used to assess the effects of bkme on aquatic biota, with particular emphasis in fish. however, few studies have been focused on effluents from pulp mill plants processing eucalyptus. the relative proportion of cytochrome p450 (cyp1a) ...201020954041
isolation and characterization of new potential probiotic bacteria based on quorum-sensing system.this work was aimed at identifying strains which can degrade quorum-sensing (qs) molecules from fish gut, with properties suitable for use as probiotic in aquaculture.201020955192
neuromodulatory effect of gnrh on the synaptic transmission of the olfactory bulbar neural circuit in goldfish, carassius auratus.gonadotropin-releasing hormone (gnrh) is well known as a hypophysiotropic hormone that is produced in the hypothalamus and facilitates the release of gonadotropins from the pituitary gonadotropes. on the other hand, the functions of extrahypothalamic gnrh systems still remain elusive. here we examined whether the activity of the olfactory bulbar neural circuits is modulated by gnrh that originates mainly from the terminal nerve (tn) gnrh system in goldfish (carassius auratus). as the morphologic ...201020962071
rate of gain of heat-tolerance in goldfish (carassius auratus). 194620997984
characterization of fish irf3 as an ifn-inducible protein reveals evolving regulation of ifn response in vertebrates.in mammals, ifn regulatory factor (irf) 3 is a critical player in modulating transcription of type i ifn and ifn-stimulated genes (isgs). in this study, we describe the roles of crucian carp (carassius auratus l.) irf3 in activating fish ifn and isgs. fish irf3 exhibits a large sequence divergence from mammalian orthologs. whereas mammalian irf3 is constitutively expressed, fish irf3 protein is significantly upregulated by ifn, poly-ic, and other stimuli known as ifn inducers in mammals. the ifn ...201021084665
simple and direct detection of aeromonas hydrophila infection in the goldfish, carassius auratus (l.), by dot blotting using specific monoclonal antibodies.a combination of eight isolates of aeromonas hydrophila was used to produce monoclonal antibodies (mabs). ten different groups of mabs specific to aeromonas were selected. the first five groups of mabs demonstrated high specificity and bound to only one or two isolates of a. hydrophila. the sixth and the seventh groups of mabs were a. hydrophila specific. they recognized seven of eight a. hydrophila isolates (ah1, 2, 3, 4, 5, 6, 8); however, the mab in the seventh group also showed cross-reactiv ...201021091724
not only students can express alcohol dehydrogenase: goldfish can too!this article describes a novel approach to study the metabolic regulation of the respiratory system in vertebrates that suits physiology lessons for undergraduate students. it consists of an experimental demonstration of the goldfish's (carassius auratus) adaptations to anoxia. the goldfish is one of the few vertebrates showing strong enzymatic plasticity for the expression of alcohol dehydrogenase (adh), which allows it to survive long periods of severe anoxia. therefore, we propose two simple ...201021098391
attempt at cloning high-quality goldfish breed 'ranchu' by fin-cultured cell nuclear transplantation.the viability of ornamental fish culture relies on the maintenance of high-quality breeds. to improve the profitability of culture operations we attempted to produce cloned fish from the somatic nucleus of the high-quality japanese goldfish (carassius auratus auratus) breed 'ranchu'. we transplanted the nucleus of a cultured fin-cell from an adult ranchu into the non-enucleated egg of the original goldfish breed 'wakin'. of the 2323 eggs we treated, 802 underwent cleavage, 321 reached the blastu ...201221106134
characterization and functional analysis of goldfish (carassius auratus l.) interleukin-10.this is the first report of comprehensive functional analysis of an interleukin-10 in bony fish. quantitative expression analysis of goldfish il-10 revealed the greatest mrna levels in the spleen tissues, peripheral blood leukocytes and granulocytes. the stimulation of cells with recombinant goldfish (rg) tnfα2 significantly reduced il-10 mrna levels in granulocytes and monocytes of the goldfish. to functionally assess the goldfish il-10, we generated a recombinant form of the molecule (rgil-10) ...201021111485
the application of hcg, cph and ovopel in successful artificial reproduction of goldfish (carassius auratus auratus) under controlled conditions.artificial reproduction of fish is one of the main goals of aquaculture production. the aim of this study is to optimize the method of goldfish reproduction under controlled conditions by comparing the effectiveness of carp pituitary homogenate (cph), ovopel and human chorionic gonadotropin (hcg), administered as a one-off dose and inducing two spawns in the same fish within a short time period. goldfish spawners were stimulated with hcg, cph and ovopel, and the results were compared to the fish ...201021134006
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