Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| hyperoxia results in transient oxidative stress and an adaptive response by antioxidant enzymes in goldfish tissues. | the effects of hyperoxia on the status of antioxidant defenses and markers of oxidative damage were evaluated in goldfish tissues. the levels of lipid peroxides, thiobarbituric acid reactive substances, carbonyl proteins and the activities of some antioxidant enzymes were measured in brain, liver, kidney and skeletal muscle of goldfish, carassius auratus l., over a time course of 3-12 h of hyperoxia exposure followed by 12 or 36 h of normoxic recovery. exposure to high oxygen resulted in an accu ... | 2005 | 15896673 |
| a multiwall carbon nanotubes film-modified carbon fiber ultramicroelectrode for the determination of nitric oxide radical in liver mitochondria. | a novel chemically modified electrode based on the multiwall carbon nanotubes (mwnts) film-coated carbon fiber ultramicroelectrode (cfue) has been described for the determination of nitric oxide radical (.no). the electrochemical behaviors of mwnts-modified cfue have been characterized in 0.2 mmol l(-1) k(4)fe(cn)(6) and 0.1 mol l(-1) kcl solution. the nafion film was used to avoid some electroactive interferences. the amount of nafion was optimized, and some possible interferents [such as nitri ... | 2005 | 15713564 |
| electron paramagnetic resonance evidence of hydroxyl radical generation and oxidative damage induced by tetrabromobisphenol a in carassius auratus. | tetrabromobisphenol a (tbbpa) is one of the most widely used brominated flame retardants (bfrs). to confirm its putative oxidative stress-inducing activity, freshwater fish carassius auratus were injected intraperitoneally with tbbpa. one experiment lasted 3h to 28 days after a single injection of 100mg/kg tbbpa, and the other lasted 24h after a single injection of 0-300 mg/kg tbbpa. reactive oxygen species (ros) were trapped by phenyl-tert-butyl nitrone (pbn) and detected by electron paramagnet ... | 2005 | 16054708 |
| catalase inhibition by amino triazole induces oxidative stress in goldfish brain. | the effects of in vivo inhibition of catalase by 3-amino 1,2,4-triazole (amt) on the levels of damage products resulting from reactive oxygen species attack on proteins and lipids as well as on the activities of five antioxidant and associated enzymes were studied in the brain of goldfish, carassius auratus. intraperitoneal injection of amt at a concentration of 0.1 mg/g wet weight caused a gradual decrease in brain catalase activity over 72 h, whereas higher amt concentrations (0.5 or 1.0 mg/g) ... | 2005 | 16023088 |
| are the cannabinoids involved in bony fish reproduction? | following the discovery of two cb1 genes in the fish fugu rubripes, investigations on the phylogeny of endocannabinoids have indicated that this system is highly conserved. our study demonstrated that cb1 receptors are expressed in the cns and gonads of two teleosts, carassius auratus and pelvicachromis pulcher, and they show a high percentage of sequence identity with fugu rubripes cb(1a) and danio rerio cb1. by means of immunohistochemistry for cb1, sgnrh, and th, we found a codistribution of ... | 2005 | 15891041 |
| direction selectivity in the goldfish tectum revisited. | responses of direction-selective (ds) ganglion cells (gcs) were recorded extracellularly from their axon terminals in the superficial layer of the tectum opticum (to) of immobilized goldfish, carassius auratus gibelio (bloch). directional tuning curves were measured with contrast edges moving in 12 or more different directions across the receptive field (rf). all directional tuning curves had cardioid-like appearance, their acceptance angles amounted to somewhat more than 180 degrees . according ... | 2005 | 16154933 |
| mechanoreceptive and nociceptive responses in the central nervous system of goldfish (carassius auratus) and trout (oncorhynchus mykiss). | nociceptive responses to a repetitive pin-prod stimulus in goldfish and trout were assessed. single unit recordings were taken from the spinal cord, cerebellum, tectum, and telencephalon. neuronal responses were elicited in all these regions of the central nervous system in both species of fish during brush (mechanoceptive) and pin-prod (nociceptive) stimuli. in addition, in trout, a heated prod stimulus was used. mechanoreceptive and nociceptive neuronal responses to various stimuli were elicit ... | 2005 | 16139775 |
| hearing in fishes under noise conditions. | our current knowledge on sound detection in fishes is mainly based on data acquired under quiet laboratory conditions. however, it is important to relate auditory thresholds to background noise in order to determine the signal-detecting abilities of animals in the natural environment. we investigated the influence of two noise levels within the naturally occurring range on the auditory sensitivity of two hearing specialists (otophysines) and a hearing generalist. audiograms of the goldfish caras ... | 2005 | 15735936 |
| growth and dietary utilisation in goldfish (carassius auratus linn.) fed diets formulated with various local agro-produces. | during a 60 d feeding trial, goldfish (carassius auratus) measuring 1.66+/-0.02 g by weight and 4.2+/-0.02 mm by length were fed diets containing 23.34%, 26.21%, 29.30%, 32.24% and 42.53% crude protein in feeds i through v respectively. the four formulated-diets (feeds i, ii, iv and v) contained groundnut oil-cake, wheat bran, soybean meal, fish meal, cod liver oil, vegetable oil and vitamin-mineral mixture with tapioca as binder and a commercially-available diet (feed iii) procured from the mar ... | 2005 | 15588775 |
| the endocannabinoid system in the brain of carassius auratus and its possible role in the control of food intake. | cannabinoid receptors and the endocannabinoids anandamide and 2-arachidonoylglycerol have been suggested to regulate food intake in several animal phyla. orthologs of the mammalian cannabinoid cb(1) and cb(2) receptors have been identified in fish. we investigated the presence of this endocannabinoid system in the brain of the goldfish carassius auratus and its role in food consumption. cb(1)-like immunoreactivity was distributed throughout the goldfish brain. the prosencephalon showed strong cb ... | 2005 | 16135090 |
| [free radical generation and lipid peroxidation induced by 2,4-dichlorophenol in liver of carassius auratus]. | free radical generation in liver of larval fish (carassius auratus) after 2,4-dichlorophenol (2,4-dcp) injected intraperitoneally 24 h was studied with electron paramagnetic resonance (epr) spin trapping technique and alpha-phenyl-n-tert-butylnitrone (pbn) as spin trap reagent. the results show that there is a significant increase of free radical generation in the liver of the fish treated with 2,4-dcp (0.025, 0.05, 0.5, 5, 25 mg/kg) compared with that of the control (p<0.05). the free radical g ... | 2005 | 16124465 |
| [mitochondrial dna variation in diploid and triploid forms of silver crucian carp carassius auratus gibelio]. | variation of two mtdna fragments amplified in polymerase chain reaction was compared by the rflp method in a population of the sympatric bisexual (diploid) and gynogenetic (triploid) forms of silver crucian carp. the mtdna haplotypes of all individuals differed in at least 2.5% of nucleotide substitutions and fall into two phylogroups. all gynogenetic individuals have haplotypes of a single phylogroup, whereas haplotypes of diploid fish belonged to both mtdna phylogroups. this testifies to a pos ... | 2005 | 16080606 |
| heavy metal accumulation and tissue damage in goldfish carassius auratus. | 2005 | 16402311 | |
| catecholamine depletion modulates serum lh levels, gad67 mrna, and gaba synthesis in the goldfish. | it is established that dopamine inhibits while gaba stimulates lh release in goldfish. in this study, we examine dopaminergic regulation of gabaergic activity in the hypothalamus of early recrudescent female goldfish (carassius auratus). we utilize a unique technique that permits concomitant quantification and correlation of in vivo gad65 and gad67 mrna with gaba synthesis rate in response to decreased dopamine levels. catecholamine depletion was achieved by treatment with alpha-methyl-para-tyro ... | 2005 | 15639145 |
| pentachlorophenol reduces b lymphocyte function through proinflammatory cytokines in carassius auratus. | cytokines and igm levels are important parameters in the acquired immunity of fish. in the present study, the effect of pentachlorophenol (pcp) on the expression of proinflammatory cytokines, tnf-alpha (tumor necrosis factor-alpha) and il-1beta (interleukin 1) mrna levels of crucian carp, were determined by semi-quantitative reverse transcriptase-polymerase chain reaction (rt-pcr) method. to put the deduction of pcp's immunotoxicity on b cell function, b cells secretion of igm under exposure of ... | 2005 | 15621336 |
| gabaergic modulation of the expression of genes involved in gaba synaptic transmission and stress in the hypothalamus and telencephalon of the female goldfish (carassius auratus). | gaba is one of the most abundant neurotransmitters in the vertebrate central nervous system and is involved in neuroendocrine processes such as development, reproduction, feeding and stress. to examine the effect of gaba on gene expression in the brain, we used a cdna macroarray containing 26 genes involved in gaba synaptic transmission (gaba receptor subunits, gaba transporters), reproduction (gonadotrophin-releasing hormone isoforms and oestrogen receptor alpha), feeding (neuropeptide y and ch ... | 2005 | 15869561 |
| molecular cloning and characterization of two types of cd8alpha from ginbuna crucian carp, carassius auratus langsdorfii. | we cloned and sequenced full-length cdnas for two types of cd8alpha from the s3n strain of ginbuna crucian carp (carassius auratus langsdorfii) and quantified the expression of cd8alpha genes after sensitization by scale grafting, employing a model system of clonal triploid ginbuna and tetraploid ginbuna-goldfish hybrids. rt-pcr yielded four different fragments of cd8alpha homologue from the s3n strain of ginbuna and these sequences were classified into two groups. the two types of ginbuna cd8al ... | 2005 | 15854681 |
| isolation, cloning, sequencing of brain type aromatase and its expression in male and female wrasse, pseudolabrus sieboldi. | pseudolabrus sieboldi, wrasse being a diurnal spawner provides a good opportunity to study the endocrine mechanism of estrogen formation in brain and gonads. moreover, an extremely large amount of e2 was produced in serum and testis of wrasse. it is assumed that the presence of e2 may play a major role in diurnal gametogenesis in male fish. in this study brain type aromatase have been isolated, cloned and sequenced from the brain of wrasse. further, the expression pattern of brain type aromatase ... | 2005 | 20035447 |
| temperature alters the respiratory surface area of crucian carp carassius carassius and goldfish carassius auratus. | we have previously found that the gills of crucian carp carassius carassius living in normoxic (aerated) water lack protruding lamellae, the primary site of o(2) uptake in fish, and that exposing them to hypoxia increases the respiratory surface area of the gills approximately 7.5-fold. we here examine whether this morphological change is triggered by temperature. we acclimated crucian carp to 10, 15, 20 and 25 degrees c for 1 month, and investigated gill morphology, oxygen consumption and the c ... | 2005 | 15767311 |
| [screen of differentially expressed genes between gastrula embryos and tail bud embryos in gynogenetic gibel carp (carassius auratus gibelio)]. | suppression subtractive hybridization (ssh) cdna plasmid libraries were constructed between gastrula embryos and tail bud embryos in gynogenetic gibel carp (carassius auratus gibelio). 739 and 816 pcr positive clones were respectively selected to perform dot blot, and 72 dot blot positive clones and 98 dot blot positive clones were obtained from the ssh plasmid libraries specific for gastrula embryos and tail bud embryos. sequencing analysis and database searches indicated that there were 19 kno ... | 2005 | 15931786 |
| sex steroid regulation of brain glutamic acid decarboxylase (gad) mrna is season-dependent and sexually dimorphic in the goldfish carassius auratus. | gaba, the major inhibitory neurotransmitter of the vertebrate brain, has been shown to play an important role in vertebrate reproduction by regulating lh release and sexual behavior. we have studied the expression of the gaba-synthesizing enzyme, glutamic acid decarboxylase (gad), in goldfish throughout the reproductive cycle in may (mature), november (early gonadal recrudescence) and february (late gonadal recrudescence) and in response to implanted sex steroids. levels of gad67 and gad65 mrna ... | 2005 | 16226340 |
| the effects of spatial food distribution and group size on foraging behaviour in a benthic fish. | animals foraging in heterogeneous environments benefit from information on local resource density because it allows allocation of foraging effort to rich patches. in foraging groups, this information may be obtained by individuals through sampling or by observing the foraging behaviour of group members. we studied the foraging behaviour of goldfish (carassius auratus) groups feeding in pools on resources distributed in patches. first, we determined if goldfish use sampling information to disting ... | 2005 | 15908140 |
| using the nucleolar biomarker and the micronucleus test on in vivo fish fin cells. | this study was aimed at developing the nucleolar biomarker and the micronucleus test on in vivo fish fin cells for assessing water cytotoxicity and genotoxicity. both biomarkers can be used either jointly or separately on fins of the same fish during the experiment. for studying the nucleolar characteristics, small pieces of the fin edge were cut several times during 30-180 min of fish exposure. for micronucleus testing, the fin tissue regenerating after its cutting was investigated after 2-5 da ... | 2005 | 15978290 |
| a novel soluble form of the csf-1 receptor inhibits proliferation of self-renewing macrophages of goldfish (carassius auratus l.). | macrophage colony-stimulating factor (csf-1) is the principal regulator of the survival, proliferation, and differentiation of macrophages and their precursors. csf-1 activity is tightly controlled through mechanisms regulating gene expression of csf-1 and its membrane-bound receptor (csf-1r), as well as by receptor-mediated endocytosis, metabolic processing, and inhibition of downstream signaling. herein we describe a novel mechanism for control of csf-1 activity. spontaneously growing goldfish ... | 2005 | 15978283 |
| perfluorooctane sulfonic acid and organohalogen pollutants in liver of three freshwater fish species in flanders (belgium): relationships with biochemical and organismal effects. | a perfluorooctane sulfonic acid (pfos) assessment was conducted on gibel carp (carassius auratus gibelio), carp (cyprinus carpio), and eel (anguilla anguilla) in flanders (belgium). the liver pfos concentrations in fish from the ieperlee canal (boezinge, 250-9031 ng/g wet weight, respectively) and the blokkersdijk pond (antwerp, 633-1822 ng/g wet weight) were higher than at the zuun basin (sint-pieters-leeuw, 11.2-162 ng/g wet weight) and among the highest in feral fish worldwide. eel from the o ... | 2005 | 15963371 |
| inhibitory effects of pituitary adenylate cyclase-activating polypeptide (pacap) and vasoactive intestinal peptide (vip) on food intake in the goldfish, carassius auratus. | pituitary adenylate cyclase-activating polypeptide (pacap) has a similar structure to that of vasoactive intestinal peptide (vip) and both the polypeptides belong to the same molecular group, the secretin-glucagon superfamily. pacap and vip have possible potency as hypothalamic factors mediating the release of pituitary hormones in the fish pituitary. however, the roles of pacap and vip in the central nervous systems of fish have not yet been made clear. recently, it was reported that pacap and/ ... | 2005 | 16112400 |
| toxoplasma gondii does not persist in goldfish (carassius auratus). | recent reports of toxoplasmosis in marine mammals raise concern that cold-blooded marine animals are a potential source of toxoplasma gondii infection. to examine the transmissibility of t. gondii to fish, we observed the development of t. gondii tachyzoites inoculated into oviduct epithelial cells of goldfish (carassius auratus) microscopically in vitro. further, the survival period of tachyzoites inoculated into goldfish muscle was bioassayed in mice and through pcr analysis. in cell cultures ... | 2005 | 16539041 |
| construction and characterization of a bac library for carassius auratus gibelio, a gynogenetic polyploid fish. | 2005 | 16293142 | |
| hydroxyl radical production and oxidative damage induced by cadmium and naphthalene in liver of carassius auratus. | freshwater goldfish (carassius auratus) were exposed to cadmium (cd) from 0 to 5 mg/l, and naphthalene (nap) from 0 to 50 mg/l. twenty-four hours after the exposure, reactive oxygen species (ros) was trapped by phenyl-tert-butyl nitrone and detected by electron paramagnetic resonance (epr). protein carbonyl (pco) and lipid peroxidation (lpo) content were determined. the activities of superoxide dismutase (sod) and catalase (cat) were also measured. the epr spectra signals were characterized by p ... | 2005 | 15792630 |
| [effects of long-term exposure of low level zinc and zn-edta complex on zinc accumulation and antioxidant defense system in liver of carassius auratus]. | the effects of low level zinc(zn2+)and its edta complex on zinc accumulation and antioxidant enzymes,such as catalase (cat), superoxide dismutase (sod), glutathione s-transferase (gst), glutathione peroxidase (gpx) in the liver of carassius auratus are studied after 40 day exposure. the results show that zinc accumulation is significantly increased with higher zinc concentration. when fish are exposed to zn-edta, the zinc accumulation in the liver of fish increased slightly according to the cont ... | 2005 | 15859432 |
| differential expression of vasa rna and protein during spermatogenesis and oogenesis in the gibel carp (carassius auratus gibelio), a bisexually and gynogenetically reproducing vertebrate. | the rna helicase vasa is a germ cell marker in animals, and its homolog in vertebrates to date has been limited to bisexual reproduction. we cloned and characterized cagvasa, a vasa homolog from the gibel carp, a fish that reproduces bisexually or gynogenetically. cagvasa possesses 14 rgg repeats and eight conserved motifs of vasa proteins. in bisexually reproducing gibel carp, vasa is maternally supplied and its zygotic expression is restricted to gonads. by in situ hybridization on testicular ... | 2005 | 15880437 |
| partial cloning and expression of mrna coding choline acetyltransferase in the spinal cord of the goldfish, carassius auratus. | choline acetyltransferase (chat, ec 2.3.1.6) synthesizes a neurotransmitter, acetylcholine in cholinergic neurons. chat is considered to be the most specific marker for cholinergic neurons. to obtain a better marker of the neurons, as the first step, we isolated a partial chat cdna from the goldfish (carassius auratus) brain by rt-pcr methods. the partial cdna of the goldfish chat was composed of 718 nucleotides. the amino acid sequence of the goldfish chat is approximately 70% identical to thos ... | 2005 | 15951213 |
| non-invasive detection and quantification of the parasitic ciliate ichthyophthirius multifiliis by real-time pcr. | the main parasitic threat to freshwater fish is the ciliate ichthyophthirius multifiliis. we developed a real-time pcr assay using sybr green intercalating fluorescent dye for rapid detection and quantification of i. multifiliis. this non-invasive assay was based on the quantification of i. multifiliis free-swimming stages from filtered water samples, and thus made it possible to preserve host individuals. an alignment of 18s rdna sequences of i. multifiliis and related species of the ciliate or ... | 2005 | 16119894 |
| identification of a spindlin homolog in gibel carp (carassius auratus gibelio). | spindlin has been suggested to play an important role during the transition from oocyte maturation to embryo development in mouse, but its homolog similar to the mouse spindlin in molecular and expression characterization has not been identified up to now in other vertebrates. in this study, a full length of cdna sequence is cloned and sequenced from the gibel carp (carassius auratus gibelio). it contains 1240 nucleotides with an open reading frame of 771 nt encoding 257 amino acids. based on it ... | 2005 | 15939319 |
| gonadotropin-releasing hormone induction of apoptosis in the testes of goldfish (carassius auratus). | apoptosis, or programmed cell death, can occur via death receptor or mitochondrial pathways. normal spermatogenesis in mammals involves apoptosis mediated, in part, by the death receptor fas and its ligand. the regulation of programmed cell death in the gonads has been shown to be dependent on a number of locally produced factors, including gnrh. whereas the role of gnrh in the control of apoptosis and follicular atresia has been documented in the mammalian ovary, gnrh regulation of testicular a ... | 2005 | 15564332 |
| a globin gene of ancient evolutionary origin in lower vertebrates: evidence for two distinct globin families in animals. | hemoglobin, myoglobin, neuroglobin, and cytoglobin are four types of vertebrate globins with distinct tissue distributions and functions. here, we report the identification of a fifth and novel globin gene from fish and amphibians, which has apparently been lost in the evolution of higher vertebrates (amniota). because its function is presently unknown, we tentatively call it globin x (gbx). globin x sequences were obtained from three fish species, the zebrafish danio rerio, the goldfish carassi ... | 2005 | 15356282 |
| characterization of the leukemia inhibitory factor receptor in the goldfish (carassius auratus). | the cytokine leukemia inhibitory factor (lif) and its receptor lifr have been extensively characterized in mammals. lif has been shown to mediate the proliferation, differentiation and activation of a number of cell types in various tissues. this paper reports on the identification of a novel lifr isolated from goldfish (carassius auratus) macrophages. goldfish lifr shares a 26% amino acid sequence identity with mammalian lifr sequences; however it retains all of the conserved amino acid motifs ... | 2005 | 15683914 |
| genetic relationships among members of the ichthyobodo necator complex: implications for the management of aquaculture stocks. | abstract ichthyobodo necator (costia) is a common and important flagellate parasite that infests the skin and gills of many freshwater and marine fish. costia infestations are often fatal and cause significant aquaculture losses worldwide. recently it has been demonstrated that ichthyobodo is a multispecies complex with differing host preferences. knowing if those species have broad or narrow host specificity has important implications for the management of costia. to address the question of hos ... | 2005 | 15705156 |
| molecular cloning of prepro-thyrotropin-releasing hormone cdnas from the common carp cyprinus carpio and goldfish carassius auratus. | to expand our knowledge on the evolution of prepro-thyrotropin-releasing hormone (pptrh) from fish to tetrapods, sequences of pptrh cdnas from two cyprinid teleosts, the common carp cyprinus carpio and goldfish carassius auratus, were determined. degenerate primers were designed based on the conserved regions between the zebrafish pptrh sequence identified from the zebrafish est database and the sockeye salmon pptrh sequence, and pcr amplification was performed. full-length pptrhs were confirmed ... | 2005 | 15707606 |
| assessment of estrogenic endocrine-disrupting chemical actions in the brain using in vivo somatic gene transfer. | estrogenic endocrine-disrupting chemicals abnormally stimulate vitellogenin gene expression and production in the liver of many male aquatic vertebrates. however, very few studies demonstrate the effects of estrogenic pollutants on brain function. we have used polyethylenimine-mediated in vivo somatic gene transfer to introduce an estrogen response element-thymidine kinase-luciferase (ere-tk-luc) construct into the brain. to determine if waterborne estrogenic chemicals modulate gene transcriptio ... | 2005 | 15743723 |
| sandwich immunoassay for determination of vitellogenin in golden grey mullet (liza aurata) serum as a field exposure biomarker. | vitellogenin (vtg) is a protein produced in the liver of oviparous animals in response to oestrogens. abnormal production of vtg by males, therefore, is used as a biological indicator of exposure to xeno-oestrogens. in this study, a sandwich-elisa for measuring vtg in liza aurata (golden grey mullet) was developed and validated. plasma vtg was purified from 17beta-oestradiol-injected immature individuals of mullet, by size-exclusion and ion-exchange chromatography. polyclonal antibodies against ... | 2005 | 15756602 |
| organ distribution and bioaccumulation of microcystins in freshwater fish at different trophic levels from the eutrophic lake chaohu, china. | this article reports the organ distribution and bioaccumulation of hepatotoxic microcystins (mcs) in freshwater fishes at different trophic levels from the large, shallow, eutrophic lake chaohu in september 2003, when there were heavy surface blooms of toxic cyanobacteria. among all fish, intestines and blood had the highest average content of mc-rr + mc-lr (22.0 and 14.5 microg g(-1) dw, respectively), followed by liver, bile, and kidney (7.77, 6.32, and 5.81 microg g(-1) dw, respectively), whe ... | 2005 | 15892067 |
| the human lipid regulator, gemfibrozil bioconcentrates and reduces testosterone in the goldfish, carassius auratus. | human and veterinarian pharmaceuticals have been detected in the aquatic environment for a number of years, but the potential for biological effects in exposed aquatic organism is only now being reported. the lipid regulator, gemfibrozil (gem) is detected at microg/l concentrations in domestic wastewater and ng/l concentrations in surface waters. we investigated the uptake of gem in goldfish (carassius auratus) over a 96 h time period by measuring gem in blood plasma using lc-ms/ms. results indi ... | 2005 | 15892991 |
| the residual dynamic of polycyclic aromatic hydrocarbons and organochlorine pesticides in fishponds of the pearl river delta, south china. | hong kong and south china are the most developed regions within china. the industrialization in these areas has resulted in severe environmental problems. sediment and biotic samples including tilapia (oreochromis mossambicus), bighead carp (aristichthys nobilis), grass carp (ctenopharyngodon idellus), crucian carp (carassius auratus) and mandarin fish/fresh water grouper (siniperca chuatsi) were collected from different fishponds in the pearl river delta (tanzhou, sanjiao, guangzhou, shipai, ch ... | 2005 | 15899281 |
| detection of mycobacteria in aquarium fish in slovenia by culture and molecular methods. | thirty-five aquarium fish were investigated for the presence of mycobacteria by culture and molecular methods. the following species were examined: goldfish carassius auratus auratus, guppy poecilia reticulata, 4 three-spot gourami trichogaster trichopterus, dwarf gourami colisa lalia, siamese fighting fish betta splendens, freshwater angelfish pterophyllum scalare, african cichlid fish cichlidae spp., cichlid fish microgeophagus altispinosus, cichlid fish pseudotropheus lombardoi, blue streak h ... | 2005 | 15900685 |
| evaluation of pesticides and metals in fish of the dniester river, moldova. | the dniester river is one of the largest rivers in eastern europe and receives input from numerous anthropogenic activities. in october 2001, fish samples (abramis brama, rutilus rutilus heckeli, perca fluviatilis, chondrostoma nasus, carassius auratus gibelio, and cyprinus carpio) were collected and analyzed for metals and various pesticides. maximum total ddt pesticide concentrations were between 27.7 and 34.2 ng/g wet weight in muscles and gonads, respectively. dde isomers comprised up to 70% ... | 2005 | 15914239 |
| responses of the antioxidant defenses of the goldfish carassius auratus, exposed to 2,4-dichlorophenol. | goldfish carassius auratus were exposed to 0.1mg/l of 2,4-dichlorophenol (2,4-dcp), widely used as transportation power in china, for 2, 5, 10, 20 and 40 days, while one control group was designated for each exposure group. antioxidant defenses consisting of contents of reduced glutathione (gsh) and glutathione disulfide (gssg) and activities of superoxide dismutase (sod), catalase (cat), glutathione reductase (gr), selenium-dependent glutathione peroxidase (se-gpx) and glutathione s-transferase ... | 2005 | 21783475 |
| a novel enzyme-linked immunosorbent assay system for the quantitative analysis of carassius auratus vitellogenin. | a sandwich enzyme-linked immunosorbent assay (elisa) was developed to quantitatively detect carassius auratus vitellogenin (vtg) levels. the protein levels in fish plasma are useful aquatic biomarkers of estrogenic compounds. this procedure involved an elisa using monoclonal antibodies of cvma2 and cvma7 against carassius auratus vtg, and cvma7 conjugated to horseradish peroxidase as the detection antibody. the assay range was between 1 and 401.5 ng/ml and the recovery of the vtg added to carass ... | 2005 | 15823333 |
| a separation-free amperometric immunosensor for vitellogenin based on screen-printed carbon arrays modified with a conductive polymer. | a disposable amperometric immunosensor was studied for the rapid detection of carp (carassius auratus) vitellogenin (vtg). the sensor was fabricated based on screen-printed carbon arrays (spcas) containing eight carbon working and an integrated carbon counter electrodes. to construct the sensor, a conducting polymer (poly-terthiophene carboxylic acid) was electropolymerized on the surface of working electrodes and the polymer-coated spcas was characterized by sem. horseradish peroxidase (hrp) an ... | 2005 | 15681194 |
| molecular cloning and sequence analysis of full-length growth hormone cdnas from six important economic fishes. | in this study,the full-length cdnas of gh (growth hormone) gene was isolated from six important economic fishes, siniperca kneri, epinephelus coioides, monopterus albus, silurus asotus, misgurnus anguillicaudatus and carassius auratus gibelio bloch. it is the first time to clone these gh sequences except e. coioides gh. the lengths of the above cdnas are as follows: 953 bp, 1 023 bp, 825 bp, 1 082 bp, 1 154 bp and 1 180 bp. each sequence includes an orf of about 600 bp which encodes a protein of ... | 2005 | 15715434 |
| [parasitofauna of the silver crucian (carassius auratus gibelio bloch, 1783) catched in the carp ponds of the leczyn-włodawa lake district]. | 2004 | 524892 | |
| identification and expression analysis of two ifn-inducible genes in crucian carp (carassius auratus l.). | interferon (ifn) exerts its antiviral effects mainly through activation of a subset of ifn-stimulated genes (isg), but relatively few of fish isgs have been isolated and characterized so far. here, we report two fish isgs, termed caifi58 and caifi56, cloned from a subtractive cdna library constructed with mrnas obtained from crucian carp (carassius auratus l.) blastulae embryonic (cab) cells infected by uv-inactivated gchv and mock-infected cells. database search revealed that both isgs had a hi ... | 2004 | 14697509 |
| positive selection on multiple antique allelic lineages of transferrin in the polyploid carassius auratus. | transferrin polymorphism has been studied in the polyploid carassius auratus by cloning and sequence analysis of cdnas from its three subspecies c. auratus gibelio, c. auratus auratus, and c. auratus cuvieri. dna polymorphism of extremely high extent was shown for the transferrin gene by the 248 segregation sites among coding region sequences of its alleles. the deduced amino acid sequences of the transferrin alleles showed variable theoretical physicochemical parameters, which might constitute ... | 2004 | 15014154 |
| endocrine disruption and altered gonadal development in white perch (morone americana) from the lower great lakes region. | high prevalences of gonadal intersex have been observed in wild fish populations in areas affected by domestic and industrial effluents. for this study, fish were collected in 1998 from the cootes paradise region of hamilton harbour in western lake ontario, canada, to determine whether gonadal abnormalities, including intersex, were present in young of the year (yoy) fish. no gonadal abnormalities were observed in goldfish (carassius auratus), common carp (cyprinus carpio), gizzard shad (dorosom ... | 2004 | 15175179 |
| production of a biologically active growth hormone from giant catfish (pangasianodon gigas) in escherichia coli. | giant catfish growth hormone (gcgh) cdna was cloned and expressed in e. coli. the expected 20.5 kda protein corresponded to the mature gcgh and was efficiently expressed. this protein was produced as inclusion bodies and comprised about 20% of total cellular proteins. the recombinant hormone promoted growth when injected intramuscularly or intraperitoneally into goldfish (carassius auratus) at 0.1 or 1 microg soluble gcgh per g fish body wt per week. in addition, the recombinant gcgh inclusions ... | 2004 | 15200175 |
| molecular phylogeny of congeneric monogenean parasites (dactylogyrus): a case of intrahost speciation. | dactylogyrus species (dactylogyridae: monogenea) are a group of monogenean gill parasites that are highly specific to freshwater fish of the family cyprinidae. dactylogyrus species were sampled from 19 cyprinids and one percid collected in europe. using partial 18s rdna and its1 sequences, a phylogeny of 51 dactylogyrus species was reconstructed to investigate the patterns of parasite speciation and diversification. three main dactylogyrus lineages were recognized from all phylogenetic trees, th ... | 2004 | 15212381 |
| oral immunization of carassius auratus with modified recombinant a-layer proteins entrapped in alginate beads. | this study was focused on the utilization of a recombinant expression system to produce a unique modified subunit vaccine possessing a self-contained delivery system which could potentially improve the uptake and delivery of vaccine products as well their immunogenic potential. for this purpose the a-layer protein (at-r) associated with the fish pathogen atypical aeromonas salmonicida was cloned and modified by the genetic fusion of the protein transduction domain (mts) derived from kaposi fibro ... | 2004 | 15530693 |
| molecular cloning and characterisation of a fish pkr-like gene from cultured cab cells induced by uv-inactivated virus. | the double-stranded-rna-dependent protein kinase (pkr) is an important component in an antiviral defence pathway that is mediated by interferon (ifn) in vertebrates. previously, some important ifn system genes had been identified from an ifn-producing cab (crucian carp carassius auratus blastulae embryonic) cells after treatment with uv-inactivated gchv (grass carp haemorrhage virus). here, a fish pkr-like gene, named capkr-like, is cloned and sequenced from the same virally infected cab cells. ... | 2004 | 15312662 |
| form and function in the unique inner ear of a teleost: the silver perch (bairdiella chrysoura). | members of the teleost family sciaenidae show significant variation in inner ear and swim bladder morphology as well as in the relationship between the swim bladder and the inner ear. in the silver perch (bairdiella chrysoura), a stellifer-group sciaenid, both the saccular and utricular otoliths are enlarged relative to those in other teleosts. additionally, its swim bladder is two-chambered, and the anterior chamber surrounds the otic capsule and terminates lateral to the saccules. structure an ... | 2004 | 15236234 |
| noise-induced stress response and hearing loss in goldfish (carassius auratus). | fishes are often exposed to environmental sounds such as those associated with shipping, seismic experiments, sonar and/or aquaculture pump systems. while efforts have been made to document the effects of such anthropogenic (human-generated) sounds on marine mammals, the effects of excess noise on fishes are poorly understood. we examined the short- and long-term effects of increased ambient sound on the stress and hearing of goldfish (carassius auratus; a hearing specialist). we reared fish und ... | 2004 | 14691090 |
| effects of crude extracts of mucuna pruriens (fabaceae) and carica papaya (caricaceae) against the protozoan fish parasite ichthyophthirius multifiliis. | the ciliate ichthyophthirius multifiliis is among the most pathogenic parasites of fish maintained in captivity. in the present study, the effects of the crude methanolic extract of leaves of mucuna pruriens and the petroleum-ether extract of seeds of carica papaya against i. multifiliis were investigated under in vivo and in vitro conditions. goldfish (carassius auratus auratus) infected with the parasites were immersed for 72 h in baths with m. pruriens extract, and for 96 h in baths with c. p ... | 2004 | 14735356 |
| effects of chronic exposure of 2,4-dichlorophenol on the antioxidant system in liver of freshwater fish carassius auratus. | there were few reports on the antioxidant response of aquatic organisms exposed to 2,4-dichlorophenol (2,4-dcp). this research explored the hepatic antioxidant responses of fish to long-term exposure of 2,4-dcp for the first time. freshwater fish carassius auratus were chosen as experimental animals. the fish were exposed to six different concentrations of 2,4-dcp (0.005-1.0 mg/l) for 40 days and then liver tissues were separated for determination. as shown from the results, 40 days afterwards, ... | 2004 | 14761689 |
| effect of microcystin-lr on protein phosphatase activity in fed and fasted juvenile goldfish carassius auratus l. | fed and fasted juvenile goldfish carassius auratus (30 g body weight) were injected intraperitoneally (i.p.) with microcystin-lr (mc-lr) (125 microg/kg body weight) to determine the effect of alimentary status on the hepatic toxicity of mc-lr. the toxin accumulation pattern was similar in both fed and fasted treatments. mc-lr accumulated during the first 48 h post-injection and decreased significantly between 48 and 96 h. mc-lr accumulation induced a decrease in hepatic protein phosphatase activ ... | 2004 | 15033328 |
| identification and characterization of a glucagon receptor from the goldfish carassius auratus: implications for the evolution of the ligand specificity of glucagon receptors in vertebrates. | the structural basis of ligand selectivity of g protein-coupled receptors for metabolic hormones has been an area of intense investigation, and yet it remains unresolved. one approach to delineating the mechanism of ligand-receptor interactions is to compare the ligand specificities of receptors expressed in species that emerged at different times within vertebrate evolution. in this paper we describe the isolation, functional, and phylogenetic characterization of the glucagon receptor from the ... | 2004 | 15033912 |
| molecular characterization and ifn signal pathway analysis of carassius auratus castat1 identified from the cultured cells in response to virus infection. | type i interferon (ifn) exerts its pleiotropic effects mainly through the jak-stat signaling pathway, which is presently best described in mammals. by subtractive suppression hybridization, two fish signaling factors, jak1 and stat1, had been identified in the ifn-induced crucian carp carassius auratus l. blastulae embryonic (cab) cells after treatment with uv-inactivated grass carp hemorrhagic virus (gchv). further, the full-length cdna of stat1, termed castat1, was obtained. it contains 2926 b ... | 2004 | 14642888 |
| molecular phylogeny of the kidney-parasitic sphaerospora renicola from common carp (cyprinus carpio) and sphaerospora sp. from goldfish (carassius auratus auratus). | the genetic relatedness of two kidney-parasitic sphaerospora species was studied. although s. renicola, the causative agent of swimbladder inflammation of common carp fingerlings (cyprinus carpio), and sphaerospora sp. originating from goldfish (carassius auratus auratus) were indistinguishable on the basis of spore morphology, they were found to be genetically different as their 18s rdna sequences shared only 71.9% identical nucleotides. in the phylogenetic trees, sphaerospora sp. from goldfish ... | 2004 | 15595280 |
| resistance to change in goldfish. | resistance to change has been studied in several species such as humans, rats, and pigeons. we conducted two experiments using goldfish as subjects to examine the generality of the findings on resistance to change in a phylogenetically more primitive species. in experiment 1, five goldfish (carassius auratus) were trained on two-component multiple schedules with different variable-interval schedules in effect. when responding was disrupted by presenting free food during intercomponent intervals ... | 2004 | 15110916 |
| haematopoietic necrosis in a goldfish (carassius auratus) associated with an agent morphologically similar to herpesvirus. | one of 14 goldfish (carassius auratus) died 4 weeks after purchase and was investigated by necropsy and histological examination. routine formalin fixation of the goldfish was followed by histopathology. formalin fixed spleen and kidney from the fish was further processed by embedding in epoxy resin and examined by transmission electron microscopy (em). severe, diffuse necrosis of haematopoietic tissue in the spleen, thymus and kidney and severe, diffuse hyperplasia in the gill epithelial cells ... | 2004 | 15088985 |
| effects of growth hormone on leucine absorption, intestinal morphology, and ultrastructure of the goldish intestine. | the mechanisms whereby exogenous growth hormone modulates intestinal structure and function in fish were investigated. goldfish (carassius auratus) were fed commercial flake diet sprayed with recombinant carp growth hormone (cgh) daily for 1 month. control animals received food sprayed with the vehicle. after 1 month of daily feedings, body mass and length were determined, and animals were sacrificed to study intestinal characteristics. sections of foregut were removed after determination of tot ... | 2004 | 15644934 |
| a drastic reduction in the basal level of heat-shock protein 90 in the brain of goldfish (carassius auratus) after administration of geldanamycin. | geldanamycin (ga), a specific inhibitor of the chaperoning function of heat-shock protein 90 (hsp90), has been shown to mimic heat shock (hs) in inducing expression of hsp90, hsp72 and other hsps in unstressed mammalian cells. in the present study, intra-cerebral treatment of goldfish with ga (at a dose of 0.1 microg/g-body weight) diminished basal hsp90 level to a 30-40% level in the brain, without affecting the basal hsp72 level, as assayed 28-48 h after treatment. whole-body exposure to hs si ... | 2004 | 15572859 |
| in vivo and in vitro activities of the seed extract of piper guineense schum. and thonn. against skin and gill monogenean parasites of gold fish (carassius auratus auratus). | methanol extracts of the seeds of piper guineense (piperaceae) were active against gold fish (carassius auratus auratus l. pisces cyprinidae) monogenean parasites. the seed extract of p. guineense was administered at different concentrations (0.5-2.0 mg/l) under in vivo and in vitro conditions. there was a higher efficacy of the effects of the extracts against fish parasites under in vitro situations than under in vivo. three major compounds (piperanine, n-isobutyl (e,e)-2,4 decadienamide and de ... | 2004 | 15551399 |
| acute toxicity of hexavalent chromium in isolated teleost hepatocytes. | acute toxic effects of hexavalent chromium [cr(vi)], a widely recognised carcinogenic, mutagenic and redox active metal, were investigated in isolated hepatocytes of goldfish (carassius auratus). exposure to 250 microm cr(vi) induced a significant decrease of cell viability from 94% in controls to 88% and 84% after 30 min and 4 h of exposure, respectively. cr-toxicity was associated with a concentration-dependent stimulation of the formation of reactive oxygen species (ros). as one potential sou ... | 2004 | 15522432 |
| molecular basis of transferrin polymorphism in goldfish (carassius auratus). | transferrin (tf) polymorphism was investigated in a color variety of goldfish (carassius auratus), and its molecular basis analyzed. three tf variants (a1, a2 and b1) were identified from an inbred strain of the goldfish, of which a1 and b1 displayed a large electrophoretic difference on both native and sds-page gels. the tf cdnas corresponding to variants a1 and b1 were cloned and sequenced from a1a1, a1b1 and b1b1 individuals, and their deduced amino acid sequences were analyzed. substantial a ... | 2004 | 15521429 |
| effects of telencephalic ablation on shoaling behavior in goldfish. | the role of the telencephalon in the shoaling behavior of the goldfish, carassius auratus, was investigated. experiments were carried out in a tank divided into three compartments. subjects were introduced into the center compartment of the tank. in the activity phase, subjects swam alone, and swimming distance was used as an index of activity. in the shoaling behavior phase, a stimulus fish was introduced into one of the side compartments, and the time spent near the side compartment by the sub ... | 2004 | 15059693 |
| development of an immunosensor for the detection of vitellogenin using impedance spectroscopy. | a simple and direct immunosensor for the determination of carp (carassius auratus) vitellogenin (vtg), a female-specific protein, has been proposed based on an antibody-captured conducting polymer-coated electrode. the monoclonal antibody specific to carp (c. auratus) vtg was immobilized by covalent coupling to the carboxylic acid group on the polymer. the antibody immobilization and antibody-antigen interaction have been demonstrated by means of quartz crystal microbalance and impedance spectro ... | 2004 | 15046756 |
| coexistence of a sexual and an unisexual form stabilized by parasites. | an unisexual species (either parthenogenetic or gynogenetic form) often coexists sympatrically with a closely related anisogamous sexual species, forming a unisexual complex. this is puzzling because, all else being equal, a sexual population with the two-fold cost of sex (or the cost of producing males) cannot coexist with a unisexual population. this suggests that some ecological processes are at work to make possible the coexistence of the unisexual complex. field and laboratory studies sugge ... | 2004 | 14643188 |
| lesions of the medial pallium, but not of the lateral pallium, disrupt spaced-trial avoidance learning in goldfish (carassius auratus). | the effects of telencephalic lesions of the medial pallium (mp) and lateral pallium (lp) of goldfish on avoidance learning were studied in a two-way, shuttle response, spaced-trial avoidance conditioning situation. animals received one trial per day, a training regime that permits the assessment of avoidance learning in the absence of stimulus carry-over effects from prior trials. control and lp-lesioned goldfish exhibited significantly faster avoidance learning than mp-lesioned animals. these r ... | 2004 | 15193757 |
| enhancement of antigen uptake and antibody production in goldfish (carassius auratus) following bath immunization and ultrasound treatment. | ultrasound irradiation and hyperosmotic treatments were compared as facilitators of antigen (bsa) penetration through the skin by bath vaccination and as enhancers of the antibody response in goldfish. the kinetics of bsa penetration and accumulation into the skin, and via it to the blood, and the consequent specific stimulation of the humoral immune response, were studied. the main findings are: (1). ultrasonic treatment is more effective than hyperosmotic treatment in enhancing both antigen tr ... | 2004 | 15193392 |
| differential expression of two carassius auratus mx genes in cultured cab cells induced by grass carp hemorrhage virus and interferon. | uv-inactivated gchv (grass carp hemorrhage virus) is able to induce an antiviral state in cultured cab cells (crucian carp carassius auratus blastulae embryonic cells) via the production of interferon (ifn). in the current work, the full-length cdnas of two mx genes, termed camx1 and camx2, have been cloned and sequenced from uv-inactivated gchv-infected and still ifn-producing cab cells by suppression subtractive hybridization. their putative proteins show the characteristically structural feat ... | 2004 | 15045470 |
| increased gad67 mrna levels are correlated with in vivo gaba synthesis in the mptp-treated catecholamine-depleted goldfish brain. | the role of catecholamine neuronal input on gabaergic activity in the hypothalamus, telencephalon, optic tectum, and cerebellum was investigated in early recrudescent female goldfish (carassius auratus). a new quantitative technique was developed and validated, permitting concomitant quantification and correlational analysis of glutamic acid decarboxylase 65 (gad65), gad67, and gad3 mrna levels and in vivo gaba synthesis. catecholamine depletion was achieved by the administration of 1-methyl-4-p ... | 2004 | 15363887 |
| the endothelin/sarafotoxin-induced increase of the proliferation of undifferentiated and dmso-differentiated gem-81 goldfish erythrophoroma cells is mediated by etb receptors. | endothelins (ets) and sarafotoxins (srtxs) have been reported to exert et(b)-mediated effects on vertebrate pigment cells. gem-81 cell line, a red pigment cell-derived cutaneous tumor of the teleost carassius auratus, expresses et(b) receptors and can be differentiated with 1.5% dmso treatment, thus constituting an useful model to investigate et and srtx effects on cultured fish pigment cells. our aim was to characterize the pharmacology and biological effects mediated by et receptors in dmso-di ... | 2004 | 15357834 |
| genotoxicity of 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2h-benzotriazole (pbta-6) and 4-amino-3,3'-dichloro-5,4'-dinitro-biphenyl (addb) in goldfish (carassius auratus) using the micronucleus test and the comet assay. | 2-[2-(acetylamino)-4-[bis(2-hydroxyethyl)amino]-5-methoxyphenyl]-5-amino-7-bromo-4-chloro-2h-benzotriazole (pbta-6) and 4-amino-3,3'-dichloro-5,4'-dinitrobiphenyl (addb) are two compounds, which show strong mutagenicity toward bacteria, that have been identified as major mutagens in river water in japan. in the present study, we examined the genotoxicity of pbta-6 and addb in goldfish (carassius auratus) by the micronucleus test and single-cell gel electrophoresis (comet assay). the frequencies ... | 2004 | 15099822 |
| effects of water-soluble fractions of diesel oil on the antioxidant defenses of the goldfish, carassius auratus. | larval goldfish carassius auratus were exposed to 0.05 and 0.1 mg/l no. 20 diesel oil, widely used as transportation power in china, for 2, 4, 7, 10, 15, 25, and 40 days, while one control group was designated for each exposure group. some fish after 25 days of exposure were transferred to diluted water until the 40th day. hepatic antioxidant defense parameters of fish, including contents of reduced glutathione and glutathione disulfide and activities of superoxide dismutase, catalase, glutathio ... | 2004 | 15087171 |
| effects of cortisol on food intake, growth, and forebrain neuropeptide y and corticotropin-releasing factor gene expression in goldfish. | although elevated plasma cortisol levels and a reduction in food intake are common features of the response to stress in fish, the potential role of cortisol in the regulation of food intake in these animals is poorly understood. in this study, goldfish (carassius auratus) were fed ad libitum for 21 days diets prepared to contain 0 (control), 50 (low) or 500 (high) microg cortisol/g of food. while feeding remained unchanged in controls and in fish fed the high cortisol diet, daily food intake gr ... | 2004 | 14697310 |
| time course of brain cholinesterase inhibition and recovery following acute and subacute azinphosmethyl, parathion and carbaryl exposure in the goldfish (carassius auratus). | laboratory toxicity data contrasting mortality and brain cholinesterase inhibition in the goldfish (carassius auratus) are presented. brain cholinesterase (che) was greatly reduced after 96 h of exposure in vivo at sublethal concentrations of azinphosmethyl and parathion. the inhibition of the enzyme was dose dependent, and concentrations higher than 0.1mg/l caused more than 90% inhibition. the effect of carbaryl was less pronounced, achieving an 86% inhibition at concentrations corresponding to ... | 2004 | 15041264 |
| effects of nonylphenol and phytoestrogen-enriched diet on plasma vitellogenin, steroid hormone, hepatic cytochrome p450 1a, and glutathione-s-transferase values in goldfish (carassius auratus). | the effects of nonylphenol (np) on plasma vitellogenin (vtg) and steroid hormone values, as well as hepatic cytochrome p450 1a (cyp1a) and glutathione-s-transferase (gst) activities, were measured in goldfish (carassius auratus) fed a diet with a low (formulated diet, fd) or high (commercial diet, cd) content of phytoestrogens, including genistein and daidzein. male goldfish with secondary sexual characteristics were exposed to nominal np concentrations of 0.1, 1.0, 10, and 100 microg/l in the w ... | 2004 | 15027619 |
| effects of running water on brainstem lateral line responses in trout, oncorhynchus mykiss, to sinusoidal wave stimuli. | we investigated how single units in the medial octavolateralis nucleus of the rainbow trout, oncorhynchus mykiss, respond to a 50-hz vibrating sphere in still and running water. four types of units were distinguished. type mi units ( n=16) were flow-sensitive; their ongoing discharge rates either increased or decreased in running water, and as a consequence, responses of these units to the vibrating sphere were masked if the fish was exposed to water flow. type mii units ( n=7) were not flow-sen ... | 2004 | 14997333 |
| proteome comparative analysis of gynogenetic haploid and diploid embryos of goldfish (carassius auratus). | recently, it was found that in the gynogenetic haploid and diploid embryos of goldfish, which have exactly the same genome, the haploid condition results in obstruction of gene expression and abnormal development while the diploid embryos have normal gene expression and development. a diploid-dependent regulatory apparatus was proposed to regulate gene expression. to study the difference at the protein expression level of the embryos of haploid and diploid in development, we extracted the total ... | 2004 | 14730685 |
| tissue-specific cu bioaccumulation patterns and differences in sensitivity to waterborne cu in three freshwater fish: rainbow trout (oncorhynchus mykiss), common carp (cyprinus carpio), and gibel carp (carassius auratus gibelio). | rainbow trout (oncorhynchus mykiss), common carp (cyprinus carpio) and gibel carp (carassius auratus gibelio) were exposed to copper (1-20 microm) in softened antwerp city tap water at ph 7.3 +/- 0.1 and with a water hardness of 292.4 +/- 8.1 mg/l caco(3) (ca 100.8 +/- 3.0 mg/l; mg 11.0 +/- 0.2 mg/l). lc50s (96 h) were determined and copper accumulation in gills, liver, and kidney assessed over a 10-day period. rainbow trout (96 h lc50: 3.3 microm/210 microg/l) were three times more sensitive to ... | 2004 | 15550275 |
| pluripotency of cryopreserved blastomeres of the goldfish. | to examine the pluripotency of cryopreserved blastomeres, we transplanted them into blastula. donor blastomeres were prepared from blastula of goldfish (carassius auratus) and cryopreserved in liquid nitrogen for two months. fifty-five percent and 44% of blastomeres survived after thawing. cryopreserved blastomeres were transplanted to the blastula of triploid crucian carp (c. a. longsdorfii), which reproduces gynogenetically in nature. at four days after the operation, resultant chimeric embryo ... | 2004 | 14743512 |
| a c-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish. | oocyte maturation and egg fertilization in both vertebrates and invertebrates are marked by orchestrated cytoplasmic translocation of secretory vesicles known as cortical granules. it is thought that such redistribution of cellular content is critical for asymmetrical cell division during early development, but the mechanism and regulation of the process is poorly understood. here we report the identification, purification and cdna cloning of a c-type lectin from oocytes of a freshwater fish spe ... | 2004 | 14732397 |
| effect of microcystin-lr on protein phosphatase activity and glycogen content in isolated hepatocytes of fed and fasted juvenile goldfish carassius auratus l. | toxic effects of microcystin-lr were studied in hepatocytes isolated from fed and fasted juvenile goldfish carassius auratus (30 g body weight). the hepatocytes were incubated with 10 microgmc-lr l(-1) during 4 h. mc-lr induces no effect in terms of cell number and viability. the toxin accumulation pattern was different in fed and fasted treatments. mc-lr accumulated more rapidly in 'fasted' cells where the highest concentration was observed by 1 h of exposure. it was delayed to 4 h in the 'fed' ... | 2004 | 15530975 |
| effects of atrazine on sex steroid dynamics, plasma vitellogenin concentration and gonad development in adult goldfish (carassius auratus). | sexually mature goldfish (carassius auratus) of both sexes were exposed to two doses (100 and 1000 microg/l) of the widely used herbicide atrazine (2-chloro-4-ethylamino-6-isopropylamino-s-triazine) for a period of 21 days and effects on the concentrations of gonad and plasma sex steroids (testosterone (t), 17beta-estradiol (e2) and 11-ketotestosterone (11-kt)), plasma vitellogenin (vtg) and gonad histo-morphology assessed. atrazine did not show any obvious estrogenic effect in males, as determi ... | 2004 | 15168945 |
| enzyme treatment of trypanosoma danilewskyi (laveran and mesnil) increases its susceptibility to lysis by the alternative complement pathway of goldfish, carassius auratus (l.). | this study examined whether in vitro-cultured trypanosoma danilewskyi were susceptible to lysis in the presence or absence of anti-parasite antibodies and complement. cultured trypanosomes were resistant to lysis by either immune or non-immune goldfish serum. however, trypanosomes treated with the proteolytic enzyme trypsin, which destroys surface proteins of the parasites, became susceptible to lysis when exposed to either immune or non-immune goldfish serum. the lysis by goldfish serum was dep ... | 2004 | 15139906 |
| cell culture of clonal ginbuna crucian carp hematopoietic cells: differentiation of cultured cells into erythrocytes in vivo. | we cultivated kidney hematopoietic cells from clonal triploid ginbuna crucian carp, carassius auratus langsdorfii. proliferating cells from hematopoietic cell cultures were harvested and injected into tetraploid hybrids (clonal triploid ginbuna and goldfish hybrid) which possess three sets of chromosomes from a triploid clone and a haploid set of chromosomes from goldfish (carassius auratus). after injection of cultured triploid cells (donor cells), blood samples were obtained from tetraploid hy ... | 2004 | 15183027 |
| kinetic characters and resistance to inhibition of crude and purified brain acetylcholinesterase of three freshwater fishes by organophosphates. | acetylcholinesterase (ache) was purified from the brain of three fresh-water fishes, topmouth gudgeon (pseudorasbora parva), goldfish (carassius auratus auratus) and rainbow trout (oncorrhychus mykiss, formerly named salmo gairdneri) by peg2000/phosphate-salt two phases extraction, deae-sephadex a-50 and sephadex g-200 chromatography. kinetic characters and resistance to inhibition of crude and purified enzymes by organophosphates were then studied. although the crude enzyme from the trout displ ... | 2004 | 15177947 |
| peptide effects on social behavior: effects of vasotocin and isotocin on social approach behavior in male goldfish (carassius auratus). | the authors measured the effects of centrally infused peptides on social approach behaviors in goldfish (carassius auratus), a social teleost. vasotocin (vt) inhibited approach responses toward the visual stimuli of conspecifics in the absence of aggressive or sexual olfactory contextual cues in males, and a v1 receptor antagonist stimulated such responses, at least in males that were not highly social in baseline conditions, as did isotocin (it). in the absence of social stimuli, vt did not aff ... | 2004 | 15174940 |
| [effects of copper (cu2+) and cu-edta complex on the induction of hsp70 in the fish brain]. | the effects of copper (cu2+) and cu-edta complex on the induction of hsp70 in the fish brain were studied. after the fish (carassius auratus) were exposed to the experimental concentrations for forty days, the sds-page and western blotting techniques were used to detect the expression level of hsp70 in the fish brain. the results showed that cu2+ and cu-edta complex can induce the expression of hsp70 significantly. comparing with the test groups exposed to cu2+, the cu-edta complex had changed t ... | 2004 | 15327262 |
| effects of four chlorobenzenes on serum sex steroids and hepatic microsome enzyme activities in crucian carp, carassius auratus. | four chlorobenzenes (chlorobenzene, 1,3-dichlorobenzene, 1,4-dichlorobenzene, p-chloro-methylbenzene) were administrated to the crucian carps (carassius auratus) by peritoneal injections in the laboratory for 30 days. serum testosterone and 17 beta-estradiol concentrations were detected using radioimmunology assay (ria), and the activities of two hepatic microsome enzymes, glutathione s-transferase (gst) and udp-glucuronosyltransferase (udpgt), were measured using the modified methods as describ ... | 2004 | 15294436 |
| identification of a novel c1q family member in color crucian carp (carassius auratus) ovary. | potential roles of c1q/tumor necrosis factor (tnf) superfamily proteins have been observed in vertebrate oogenesis and oocyte maturation, but no ovary-specific member has been identified so far. in this study, we have cloned and identified a novel member of c1q family with a c1q domain in the c-terminal from fully grown oocyte cdna library of color crucian carp and demonstrated that the gene might be specifically expressed in ovary and therefore designated as carassius auratus ovary-specific c1q ... | 2004 | 15253877 |