Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| plasma pharmacokinetics and muscle residue dynamics of mebendazole in carassius auratus. | mebendazole is approved for use in aquatic animals and is widely used in chinese aquaculture. we developed a pharmacokinetic and residue analysis for mebendazole levels in the goldfish (carassius auratus). plasma and muscle samples of c. auratus were taken after oral administration of 10 mg/kg mebendazole. the maximal drug plasma concentration of 0.55 mg/l was achieved at 48 hr and then declined with the elimination half-life (t1/2β ) of 7.99 hr. administration of 10 mg/kg by oral gavage for 5 s ... | 2017 | 28401566 |
| stimulatory effects of heat-killed enterococcus faecalis on cell-mediated immunity in fish. | intracellular bacterial and viral diseases are widespread in the aquaculture industry and cause serious economic losses. development of effective vaccines and adjuvants that can induce cell-mediated immunity is urgently needed for prevention of these diseases. here we report the immunostimulatory effects of probiotic bacteria ''e. faecalis'' in ginbuna crucian carp carassius auratus langsdorfii. intraperitoneal injection of heat-killed e. faecalis induced an increase in cd4-1(+) lymphocytes, cd8 ... | 2017 | 28389237 |
| the interactive effect of digesting a meal and thermal acclimation on maximal enzyme activities in the gill, kidney, and intestine of goldfish (carassius auratus). | surrounding environmental temperatures affect many aspects of ectotherm physiology. generally, organisms can compensate at one or more biological levels, or allow temperature to dictate processes such as enzyme activities through kinetic effects on reaction rates. as digestion also alters physiological processes such as enzyme activities, this study determined the interacting effect of thermal acclimation (8 and 20 °c) and digesting a single meal on maximal enzyme activities in three tissues of ... | 2017 | 28382530 |
| bacterial signatures of "red-operculum" disease in the gut of crucian carp (carassius auratus). | fish gut microbiota play important roles in fish immunity, nutrition, and the adaptation to environmental changes. to date, few studies have focused on the interactions among environmental factors, fish diseases, and gut microbiota compositions. we compared the gut bacterial communities of healthy crucian carps (carassius auratus) with those of individuals affected by "red-operculum" disease and corresponding water and sediment microbiota in four fish farm ponds. distinct gut bacterial communiti ... | 2017 | 28364130 |
| propofol as an immersion anesthetic and in a minimum anesthetic concentration (mac) reduction model in goldfish (carassius auratus). | propofol is a novel immersion anesthetic in goldfish ( carassius auratus ). objectives were to characterize propofol as an anesthetic and assess its suitability in a minimum anesthetic concentration (mac) reduction model. using a crossover design, eight goldfish were submerged in 1, 5, or 10 mg/l propofol. data included induction time, recovery time, heart rate, opercular rate, and response to supramaximal stimulation. baseline mac (dixon's up-and-down method) was determined, and 15 fish were an ... | 2017 | 28363074 |
| development of a multiplex pcr method for the simultaneous detection of four myxosporeans infecting gibel carp carassius auratus gibelio. | gibel carp carassius auratus gibelio (bloch), a commercially important freshwater-cultured fish in china, is threatened by myxosporeans, particularly thelohanellus wuhanensis, myxobolus honghuensis, m. wulii and m. turpisrotundus. here, we developed a multiplex pcr assay for simultaneous detection of these 4 myxosporeans. the specific primers for each species were designed based on the 28s rdna gene of t. wuhanensis, the its-5.8s rdna of m. honghuensis and m. wulii, and the 18s rdna gene of m. t ... | 2017 | 28357984 |
| bioconcentration and multi-biomarkers of organic uv filters (bm-dbm and od-paba) in crucian carp. | organic uv filters (ouv-fs) are increasingly used in sunscreens and personal care products. in the present work, the bioconcentration and multi-biomarker effects of butyl methoxydibenzoylmethane (bm-dbm) and ethylhexyl dimethyl p-aminobenzoate (od-paba) were investigated in crucian carp (carassius auratus). the fish were exposed to various concentrations of bm-dbm (3.88, 35.61, 181.85 and 337.15μg/l), od-paba (4.66, 53.83, 264.22 and 459.32μg/l) and their mixture (2.31+2.79, 23.69+26.18, 97.37+1 ... | 2017 | 28343007 |
| ghrelin facilitates glut2-, sglt1- and sglt2-mediated intestinal glucose transport in goldfish (carassius auratus). | glucose homeostasis is an important biological process that involves a variety of regulatory mechanisms. this study aimed to determine whether ghrelin, a multifunctional gut-brain hormone, modulates intestinal glucose transport in goldfish (carassius auratus). three intestinal glucose transporters, the facilitative glucose transporter 2 (glut2), and the sodium/glucose co-transporters 1 (sglt1) and 2 (sglt2), were studied. immunostaining of intestinal sections found colocalization of ghrelin and ... | 2017 | 28338019 |
| influence of multi-walled carbon nanotubes on the effects of roxithromycin in crucian carp (carassius auratus) in the presence of natural organic matter. | carbon nanotubes are increasingly entering the aquatic environment and may interact with other co-existing contaminants, such as antibiotics. however, whether these interactions may affect their bioavailability in aquatic organisms is the subject of considerable debate. the primary objective of this study was to assess the risks arising from the coexistence of roxithromycin (rox) and multi-walled carbon nanotubes (mwcnts) in waters containing natural organic matter (nom), focusing on the distrib ... | 2017 | 28324838 |
| first report of rahnella aquatilis infection in crucian carp carassius auratus in china. | rahnella aquatilis infection is rare in aquaculture. here, a gram-negative rod-shaped bacterium was isolated from diseased crucian carp carassius auratus in xuzhou city, jiangsu province, eastern china. the isolate was tentatively named strain kcl-5, and subsequently identified as r. aquatilis by biochemical properties and molecular techniques. the results showed that the isolate kcl-5 was most closely related to the type strain atcc33071 (= dsm4594) of r. aquatilis, which shared 99.67, 96.26 an ... | 2017 | 28322207 |
| vegetalising factor extracted from the fish swimbladder and tested on presumptive ectoderm oftriturus embryos. | vegetalising factor was isolated from swimbladder of crusian carp (carassius auratus) by solubilishing with 8 m urea the precipitate obtained after digesting the swimbladder with collagenase. the urea-soluble fraction vegetalised isolated presumptive ectoderm oftriturus gastrula and produced both undifferentiated mesodermal and endodermal cells. brief heating of the fraction changed its capacity to produce organised mesodermal tissues, such as notochord and somite, and the frequency of induction ... | 1977 | 28304885 |
| molecular phylogenetics of centrocestus formosanus (digenea: heterophyidae) originated from freshwater fish from chiang mai province, thailand. | this study aimed to investigate the morphology and reconstruct the phylogenetic relationships of centrocestus formosanus originating from 5 species of freshwater fish, i.e., esomus metallicus, puntius brevis, anabas testudineus, parambassis siamensis, and carassius auratus, in chiang mai province, thailand. sequence-related amplified polymorphism (srap) and phylogeny based on internal transcribed spacer 2 (its2) and mitochondrial cytochrome c oxidase subunit 1 (co1) were performed. the results s ... | 2017 | 28285504 |
| molecular characterization and expression analysis of toll-like receptors 5 and 22 from natural triploid carassius auratus. | innate immunity, as the most primitive and universal host defense in fish, constitutes an efficient first line of defense to combat invading microbes. toll-like receptors (tlrs) play essential roles in the innate immunity, and tlr5 and tlr22 are two important tlrs that can recognize flagellin and double stranded rna (dsrna), respectively. in this study, we identified and characterized two tlrs genes of qihe crucian carp (carassius auratus) (designated as catlr5 and catlr22). the full-length cdna ... | 2017 | 28259778 |
| diverse chlamydia-like agents associated with epitheliocystis infection in two cyprinid fish species, the common carp (cyprinus carpio l.) and the gibel carp (carassius auratus gibelio l.). | during a general annual fish health survey in natural waters and ponds, epitheliocystis infections were recorded in fingerlings of two cyprinid fish species, the cultured common carp and the wild gibel carp. benign and heavy infections were equally observed without mortality. in addition to the general health inspection of fish, histopathological examinations of infected gills and molecular biological investigations of separated epitheliocysts were performed. epitheliocysts were formed both in t ... | 2017 | 28244339 |
| opisthorchis viverrini infection in the snail and fish intermediate hosts in central vietnam. | opisthorchis viverrini, a carcinogenic fish borne fluke, requires freshwater snails and fish as intermediate hosts. opisthorchiasis is endemic in parts of southeast asia, including central and south vietnam. in this region the transmission by intermediate hosts has received little attention. therefore, freshwater snails and wild fish from bau my tho, an opisthorchiasis endemic area in binh dinh province were collected for examination of o. viverrini cercariae and metacercariae, respectively. a t ... | 2017 | 28242064 |
| effects on heavy metal accumulation in freshwater fishes: species, tissues, and sizes. | three fish species (carassius auratus, pelteobagrus fulvidraco, and squaliobarbus curriculus) were collected from xiang river near changsha city, southern china. the concentrations of heavy metals including arsenic (as), cadmium (cd), copper (cu), iron (fe), manganese (mn), lead (pb), and zinc (zn) in the muscle, gill, and liver of three species were determined by the inductively coupled plasma mass spectrometry method. one-way analysis of variance (anova) was introduced to determine the signifi ... | 2017 | 28233209 |
| irisin in goldfish (carassius auratus): effects of irisin injections on feeding behavior and expression of appetite regulators, uncoupling proteins and lipoprotein lipase, and fasting-induced changes in fndc5 expression. | irisin is a peptide cleaved from the fibronectin type iii domain containing protein 5 (fndc5) gene that is secreted predominantly by muscle cells but also by other tissues including brain and intestine. in mammals, irisin has been shown to have thermogenic actions via the modulation of uncoupling proteins (ucps) and to affect feeding and energy homeostasis via actions in brain, adipose tissue, liver, muscle and gastrointestinal tract. to examine the role of irisin on feeding and metabolism in fi ... | 2017 | 28219696 |
| microcystin bioaccumulation in freshwater fish at different trophic levels from the eutrophic lake chaohu, china. | the spatial and temporal variations of microcystins (mcs) in fishes with different trophic levels were studied monthly in lake chaohu in 2014. mcs content in muscle was highest in phytoplanktivorous hypophthalmichthys molitrix (h. molitrix), followed by omnivorous carassius auratus (c. auratus), and was lowest in herbivorous parabramis pekinensis (p. pekinensis) and carnivorous coilia ectenes (c. ectenes). mcs concentration in liver was highest in c. auratus, followed by h. molitrix, and was low ... | 2017 | 28213674 |
| comparative pharmacokinetics of enrofloxacin in healthy and aeromonas hydrophila-infected crucian carp (carassius auratus gibelio). | the comparative pharmacokinetics of enrofloxacin (enr) and its metabolite ciprofloxacin (cip) were investigated in healthy and aeromonas hydrophila-infected crucian carp after a single oral (p.o.) administration at a dose of 10 mg/kg at 25 °c. the plasma concentrations of enr and of cip were determined by hplc. pharmacokinetic parameters were calculated based on mean enr concentrations by noncompartmental modeling. in healthy fish, the elimination half-life (t1/2λz ), maximum plasma concentratio ... | 2017 | 28205238 |
| characterization of ghrelin o-acyltransferase (goat) in goldfish (carassius auratus). | ghrelin is the only known hormone posttranslationally modified with an acylation. this modification is crucial for most of ghrelin's physiological effects and is catalyzed by the polytopic enzyme ghrelin o-acyltransferase (goat). the aim of this study was to characterize goat in a teleost model, goldfish (carassius auratus). first, the full-length cdna sequence was obtained by rt-pcr and rapid amplification of cdna ends methods. two highly homologous cdnas of 1491 and 1413 bp, respectively, name ... | 2017 | 28178327 |
| allometric analysis of a morphological anti-predator trait in geographic populations of japanese crucian carp. | costly anti-predator traits tend to be expressed only in high-predation conditions. for the cyprinid fish genus carassius, deeper body depth is more adaptive to avoid predation by gape-limited piscivorous fish, but it raises swimming costs. it is therefore predicted that the relative body depth will decrease when the prey fish has reached a size larger than the predator gape-size. this prediction was tested by allometric analysis of the relation between body depth and standard length of triploid ... | 2017 | 28150742 |
| identification of the putative goldfish (carassius auratus) magnesium transporter slc41a1 and functional regulation in the gill, kidney, and intestine in response to dietary and environmental manipulations. | while magnesium requirements for teleost fish highlight the physiological importance of this cation for homeostasis, little is known regarding the molecular identity of transporters responsible for magnesium absorption or secretion. the recent characterization of the vertebrate magnesium transporter solute carrier 41a1 (slc41a1) in the kidney of a euryhaline fish has provided a glimpse of possible moieties involved in piscine magnesium regulation. the present study obtained a novel slc41a1 codin ... | 2017 | 28130070 |
| ifna2 of triploid hybrid of gold fish and allotetraploid is an intracellular antiviral cytokine against svcv and gcrv. | sterile triploid hybrids (3n = 150) of gold fish (carassius auratus red var., ♀, 2n = 100) and allotetroploid (♂, 2n = 100) display obviously improved disease resistance and much enhanced growth rate than their parents, which have been cultured widely in china. in this paper, one of the type i ifns of triploid hybrid (3nifna2) has been cloned and characterized. the full-length cdna of 3nifna2 gene consists of 715 nucleotides and the predicted 3nifna2 contains 183 amino acids. the transcription o ... | 2017 | 28126620 |
| diversity of culturable bacterial communities in the intestinal tracts of goldfish (carassius auratus) and their ability to produce n-acyl homoserine lactone. | intestinal bacteria isolated from goldfish (carassius auratus) were identified based on 16 ribosomal rna (rrna) gene sequences and screened for their ability to produce n-acyl homoserine lactone (ahl), an autoinducer of the quorum sensing (qs) system. the 230 aerobes/facultative anaerobes that were isolated comprised members of the genera aeromonas (184 isolates), citrobacter (11), enterobacter (2), shewanella (28), vagococcus (1), and vibrio (4). among these genera, the two most abundant specie ... | 2017 | 28124783 |
| proteome response of fish under multiple stress exposure: effects of pesticide mixtures and temperature increase. | aquatic systems can be subjected to multiple stressors, including pollutant cocktails and elevated temperature. evaluating the combined effects of these stressors on organisms is a great challenge in environmental sciences. to the best of our knowledge, this is the first study to assess the molecular stress response of an aquatic fish species subjected to individual and combined pesticide mixtures and increased temperatures. for that, goldfish (carassius auratus) were acclimated to two different ... | 2017 | 28109940 |
| composition of gut microbiota in the gibel carp (carassius auratus gibelio) varies with host development. | to understand how a bacteria-free fish gut ecosystem develops microbiota as the fish ages, we performed a 1-year study on the gut microbiota of hatchling gibel carp (carassius auratus gibelio). our results indicate that the gut microbial diversity increases significantly as the fish develop. the gut microbial community composition showed significant shifts corresponding to host age and appeared to shift at two time points despite consistent diet and environmental conditions, suggesting that some ... | 2017 | 28108758 |
| establishment and characterization of a heart-derived cell line from goldfish (carassius auratus). | the goldfish carassius auratus, a freshwater fish in the family cyprinidae, was one of the earliest fish to be domesticated for ornamental purposes. a cell line was established from goldfish heart (gh) tissue to create a biological monitoring tool for viral diseases. the gh cell line was optimally maintained at 25 °c in m199 medium supplemented with 10-20% fetal bovine serum. a chromosomal analysis indicated that the cell line remained diploid, with a mean chromosomal count of 100. in viral inoc ... | 2017 | 28097494 |
| glucose, amino acids and fatty acids directly regulate ghrelin and nucb2/nesfatin-1 in the intestine and hepatopancreas of goldfish (carassius auratus) in vitro. | ghrelin and nesfatin-1 are two peptidyl hormones primarily involved in food intake regulation. we previously reported that the amount of dietary carbohydrates, protein and lipids modulates the expression of these peptides in goldfish in vivo. in the present work, we aimed to characterize the effects of single nutrients on ghrelin and nesfatin-1 in the intestine and hepatopancreas. first, immunolocalization of ghrelin and nucb2/nesfatin-1 in goldfish hepatopancreas cells was studied by immunohist ... | 2017 | 28089858 |
| occurrence of parasites of the genus eustrongylides spp. (nematoda: dioctophymatidae) in fish caught in trasimeno lake, italy. | eustrongylides spp. is considered a freshwater fish zoonotic nematode. in the present study, the prevalence of eustrongylides spp. in six edible fish (european perch - perca fluviatilis, goldfish - carassius auratus, largemouth black bass - micropterus salmoides, tench- tinca tinca, carp - cyprinus carpio and sand smelt - atherina boyeri) of trasimeno lake was surveyed. the investigations were conducted from october 2014 to september 2015 and 384 specimens per species for each season were caught ... | 2016 | 28058245 |
| ghrelin modulates gene and protein expression of digestive enzymes in the intestine and hepatopancreas of goldfish (carassius auratus) via the ghs-r1a: possible roles of plc/pkc and ac/pka intracellular signaling pathways. | ghrelin, a multifunctional gut-brain hormone, is involved in the regulation of gastric functions in mammals. this study aimed to determine whether ghrelin modulates digestive enzymes in goldfish (carassius auratus). immunofluorescence microscopy found colocalization of ghrelin, ghs-r1a and the digestive enzymes sucrase-isomaltase, aminopeptidase a, trypsin and lipoprotein lipase in intestinal and hepatopancreatic cells. in vitro ghrelin treatment in intestinal and hepatopancreas explant culture ... | 2017 | 28042022 |
| elevated co2 levels increase the toxicity of zno nanoparticles to goldfish (carassius auratus) in a water-sediment ecosystem. | concerns about the environmental safety of metal-based nanoparticles (mnps) in aquatic ecosystems are increasing. simultaneously, elevated atmospheric co2 levels are a serious problem worldwide, making it possible for the combined exposure of mnps and elevated co2 to the ecosystem. here we studied the toxicity of nzno to goldfish in a water-sediment ecosystem using open-top chambers flushed with ambient (400±10μl/l) or elevated (600±10μl/l) co2 for 30days. we measured the content of zn in suspen ... | 2017 | 28040633 |
| modulation of erythromycin-induced biochemical responses in crucian carp by ketoconazole. | the individual and combined biochemical responses of erythromycin and ketoconazole have been examined in an organism representative of the aquatic environment, crucian carp (carassius auratus). the possible interactions between erythromycin and ketoconazole were investigated on the bioaccumulation and the expression of biotransformation enzymes 7-ethoxyresorufin-o-deethylase (erod) and glutathione s-transferase (gst), and an antioxidant defense enzyme superoxide dismutase (sod) in fish tissues. ... | 2017 | 28004371 |
| acute exposure to the penconazole-containing fungicide topas partially augments antioxidant potential in goldfish tissues. | penconazole is a systemic fungicide commonly used in agriculture as the commercial preparation topas. although triazole fungicides are widely found in the aquatic environment, little is known about their acute toxicity on fish. in this study we assessed the effects of short-term exposure to topas on some parameters of homeostasis of reactive oxygen species (ros), such as the levels of markers of oxidative stress and parameters of the antioxidant defense system of goldfish (carassius auratus l.). ... | 2017 | 27979696 |
| cross-reactivity of monoclonal antibodies against cd4-1 and cd8α of ginbuna crucian carp with lymphocytes of zebrafish and other cyprinid species. | we have monoclonal antibodies (mabs) against cd4-1 (6d1) and cd8α (2c3) in ginbuna crucian carp carassius auratus langsdorfii. in our previous studies we showed that 2c3 mab positive cells are the primary cell type showing specific cytotoxicity against allogeneic targets, suggesting that cd8α(+) lymphocytes in ginbuna are equivalent to cytotoxic t lymphocytes (ctls) in mammals. we further demonstrated the helper t cell function of 6d1 mab positive cells by studying mixed leukocyte culture (mlc) ... | 2016 | 27965016 |
| genome sequencing and annotation of aeromonas veronii strain ae52, a multidrug-resistant isolate from septicaemic gold fish (carassius auratus) in sri lanka. | here we report the draft genome sequence and annotation of a. veronii strain ae52 isolated from the kidney of a morbund, septicaemic gold fish (carassius auratus) in sri lanka. this clinical isolate showed resistance to multiple antimicrobials; amoxicillin, neomycin, trimethoprim-sulphonamide, chloramphenicol, tetracycline, enrofloxacin, erythromycin and nitrofurantoin. the size of the draft genome is 4.56 mbp with 58.66% of g + c content consisting 4328 coding sequences. it harbors a repertoire ... | 2017 | 27942459 |
| stellate and pyramidal neurons in goldfish telencephalon respond differently to anoxia and gaba receptor inhibition. | with oxygen deprivation, the mammalian brain undergoes hyper-activity and neuronal death while this does not occur in the anoxia-tolerant goldfish (carassius auratus). anoxic survival of the goldfish may rely on neuromodulatory mechanisms to suppress neuronal hyper-excitability. as γ-aminobutyric acid (gaba) is the major inhibitory neurotransmitter in the brain, we decided to investigate its potential role in suppressing the electrical activity of goldfish telencephalic neurons. utilizing whole- ... | 2017 | 27923876 |
| ligand-biased regulation of ptdins(3,4,5)p3-dependent signal transduction in gpcr control of pituitary hormone release. | biased signaling describes the selective activation of signal transduction cascades by structurally related ligands downstream of shared g protein-coupled receptors (gpcrs). although class i phosphoinositide 3-kinases (pi3ks) are important components of gpcr-controlled transduction networks, little is known regarding the potential for biased regulation of class i pi3k-dependent signaling. the full complement of class i pi3k catalytic subunits (p110α, p110β, p110δ, and p110γ) first appears in bon ... | 2017 | 27906555 |
| small and large number discrimination in goldfish (carassius auratus) with extensive training. | previous studies on relative quantity discrimination in birds and mammals with training procedures have employed hundreds or thousands of trials whereas studies with fish typically use dozens of trials. the goal of this study was to examine whether more extensive training improves the performance of fish tested on stimuli in the small (<4) and large (>4) number range. goldfish were trained with dot array stimuli using the ratio 0.5 (2 vs. 4, 6 vs. 12) across two blocks of training sessions with ... | 2016 | 27890598 |
| brain and intestinal expression of galanin-like peptide (galp), galanin receptor r1 and galanin receptor r2, and galp regulation of food intake in goldfish (carassius auratus). | galanin-like peptide (galp) is a 60 amino acid neuropeptide originally discovered from porcine hypothalamus, and is involved in the regulation of food intake in mammals. since its discovery, galp and its receptors (galr1 and galr2) have been characterized in mammals, but no publications are available on galp in fish and other non-mammals. the present study aimed to characterize brain and intestinal galp and its receptors using immunohistochemistry in a teleost, the goldfish (carassius auratus), ... | 2017 | 27884736 |
| determination of the gut retention of plastic microbeads and microfibers in goldfish (carassius auratus). | microplastics are ubiquitous pollutants in aquatic habitats and commonly found in the gut contents of fish yet relatively little is known about the retention of these particles by fish. in this study, goldfish were fed a commercial fish food pellet amended with 50 particles of one of two microplastics types, microbeads and microfibers. microbeads were obtained from a commercial facial cleanser while microfibers were obtained from washed synthetic textile. following consumption of the amended pel ... | 2017 | 27880921 |
| spontaneous polyploidy, gynogenesis and androgenesis in second generation (f2 ) koi cyprinus carpio × goldfish carassius auratus hybrids. | the objective of this study was to characterize the genetics of second generation (f2 ) koi cyprinus carpio × goldfish carassius auratus hybrids. spermatozoa produced by a novel, fertile f1 male were found to be diploid by flow-cytometric analysis. backcross (f1 female × c. carpio male and c. carpio female × f1 male) juveniles were triploid, confirming that female and male f1 hybrids both produced diploid gametes. the vast majority of surviving f2 juveniles was diploid and small proportions were ... | 2017 | 27861861 |
| nmr elucidation of reduced b-z transition activity of pkz protein kinase at high nacl concentration. | a z-dna binding protein (zbp)-containing protein kinase (pkz) in fish species has an important role in the innate immune response. previous structural studies of the zα domain of the pkz from carassius auratus (cazαpkz) showed that the protein initially binds to b-dna and induces b-z transition of double stranded dna in a salt concentration-dependent manner. however, the significantly reduced b-z transition activity of cazαpkz at high salt concentration was not fully understood. in this study, w ... | 2017 | 27856245 |
| histopathological and ultrastructural studies of myxobolus turpisrotundus from allogynogenetic gibel carp carassius auratus gibelio in china. | during an ongoing systematic survey on species diversity of myxozoans parasitising allogynogenetic gibel carp carassius auratus gibelio (bloch) in china, plasmodia were detected in the fins, lip, jaw, gill chamber, gill arches, operculum and oral cavity of infected fish. combining the morphological and molecular data, the present species was identified as myxobolus turpisrotundus zhang, wang, li et gong, 2010. histopathological examination revealed that despite infecting different organs, m. tur ... | 2016 | 27827339 |
| immunohistochemical characterization of toll-like receptor 2 in gut epithelial cells and macrophages of goldfish carassius auratus fed with a high-cholesterol diet. | toll-like receptors (tlrs) are a group of pattern recognition molecules that play a crucial role in innate immunity. the structural conservation of the archaic tlr system suggests that the regulation of the immune response might be similar in fish and mammals. several tlrs (tlr-1, -2, and -4) are expressed by activated macrophages, "foam cells" in human atherosclerotic lesions. to date, 20 different tlrs were identified in more than a dozen different fish species. in this study we found that fee ... | 2016 | 27818343 |
| studies on histopathological alteration of three major organs of the goldfish, carassius auratus l., of india due to myxozoan infection with special reference to scanning electron microscopic observation. | severe infections by the protozoan parasite belonging to the genera myxozoa are causing serious problems in ornamental fish reared in breeding farms. histopathological study is being used for diagnosis of the severity of diseases. myxozoan infections result in large scale histopathological damages in different fish tissues. no information is available regarding the histopathological changes of goldfish due to infection with myxozoans. the present study deals with the histopathological changes of ... | 2017 | 27815737 |
| first report of some known trichodinella epizootica šramek-hušek, 1953, <br />and tripartiella lom, 1959 from the cultured oranda gold fish (carassius auratus auratus) in india. | trichodiniasis of fishes cause economic losses in the fish industry through mortality and decrease growth rate. very few studies of these ectoparasites have been made in india. a comprehensive icthyoparasotological survey in some ornamental fish farms of different parts of india has been done since march 2014-may 2015. during the study these trichodinid parasites have been identified from the gills of oranda gold fish (carassius auratus auratus l.). they are trichodinella epizootica, tripartiell ... | 2016 | 27811658 |
| sialoglycoprotein isolated from the eggs of carassius auratus prevents bone loss: an effect associated with the regulation of gut microbiota in ovariectomized rats. | the anti-osteoporotic effect of sialoglycoprotein isolated from the eggs of carassius auratus (ca-sgp) has previously been demonstrated in vivo. however, the mechanism by which this macromolecular substance regulates bone metabolism remains unclear. given the correlation between gut microbiota and the homeostasis of bone metabolism, the current study applied real-time pcr and 16s rrna high-throughput sequencing to investigate the influence of ca-sgp on gut microbiota in ovariectomized rats. real ... | 2016 | 27808338 |
| single and combined effects of aluminum (al2o3) and zinc (zno) oxide nanoparticles in a freshwater fish, carassius auratus. | the increasing use of nanoparticles (nps) worldwide has raised some concerns about their impact on the environment. the aim of the study was to assess the toxicity of metal oxide nanoparticles, singly or combined, in a freshwater fish (carassius auratus). the fish were exposed for 7, 14, and 21 days to different concentrations of nps (10 μg al2o3.l(-1), 10 μg zno.l(-1), 10 μg al2o3.l(-1) plus 10 μg zno.l(-1), 100 μg al2o3.l(-1), 100 μg zno.l(-1), and 100 μg al2o3.l(-1) plus 100 μg zno.l(-1)). at ... | 2016 | 27787704 |
| relationship between oxidative stress and brain swelling in goldfish (carassius auratus) exposed to high environmental ammonia. | buildups of ammonia can cause potentially fatal brain swelling in mammals, but such swelling is reversible in the anoxia- and ammonia-tolerant goldfish (carassius auratus). we investigated brain swelling and its possible relationship to oxidative stress in the brain and liver of goldfish acutely exposed to high external ammonia (hea; 5 mmol/l nh4cl) at two different acclimation temperatures (14°c, 4°c). exposure to hea at 14°c for 72h resulted in increased internal ammonia and glutamine concentr ... | 2017 | 27784686 |
| morphological and molecular characterisation of myxobolus pronini n. sp. (myxozoa: myxobolidae) from the abdominal cavity and visceral serous membranes of the gibel carp carassius auratus gibelio (bloch) in russia and china. | myxozoa is a well-known economically and ecologically important group of metazoan parasites, phylogenetically related to cnidaria. high diversity of myxosporeans has been recorded in russia and china; however, most of the species were solely morphologically characterised. here, we identified a new gibel carp-infecting myxobolus species and morphologically and molecularly compared the russian and chinese isolates of this new myxosporean. | 2016 | 27782848 |
| dietary lactobacillus acidophilus modulated skin mucus protein profile, immune and appetite genes expression in gold fish (carassius auratus gibelio). | the objective of the present study was to investigate the effect of dietary lactobacillus acidophilus on skin mucus protein pattern, immune and appetite related genes expression as well as growth performance in gold fish (carassius auratus gibelio). three hundred healthy gold fish (2.5 ± 0.05) juveniles were randomly distributed in 12 glass aquariums (400-l; 25 fish per aquaria) and fed experimental diets contain different levels of l. acidophilus (0, 1.5 × 10(8), 3 × 10(8) and 6 × 10(8)) for 8 ... | 2016 | 27769690 |
| localized axis determinant in the early cleavage embryo of the goldfish, carassius auratus. | the teleost dorsoventral axis cannot be distinguished morphologically before gastrulation. in order to examine whether the yolk cell affects axis determination, we bisect early cleavage embryos of the goldfish, carassius auratus. when the vegetal yolk hemisphere is removed by bisection along the equatorial plane at the 2-cell stage, the embryos develop abnormally and exhibit a symmetrical morphology. no dorsal structures, such as notochord, somites and neural tube, differentiate and no embryonic ... | 1997 | 27747400 |
| preliminary study on the relationship between dexamethasone and pathogen susceptibility on crucian carp (carassius auratus). | dexamethasone, a known immunosuppressant, can inhibit the immune response and increase the amount of pathogen in body, but the role of dexamethasone affecting susceptibility of crucian carp (carassius auratus) to pathogen is unclear. the effects of dexamethasone on susceptibility of crucian carp to aeromonas hydrophila were investigated in this study. the fish were divided into four groups randomly and injected intraperitoneally by dexamethasone for 0 day (group d), 3 days (group c), 6 days (gro ... | 2016 | 27744057 |
| refuse leachate exposure causes changes of thyroid hormone level and related gene expression in female goldfish (carassius auratus). | to elucidate the potential thyroid disrupting effects of refuse leachate on females, female goldfish (carassius auratus) were exposed to 0.5% diluted leachates from each step of a leachate treatment process (i.e. raw leachate before treatment, after membrane bioreactor treatment, and the final treated leachate) for 21days. raw leachate exposure caused disturbances in the thyroid cascade of female fish, as evidenced by the elevated plasma 3,3',5-triiodo-l-thyronine (p<0.05) and thyroid-stimulatin ... | 2016 | 27736670 |
| an in vivo assay performed using multiple biomarkers related to testosterone synthesis and conversion for assessing the androgenic potency of refuse leachate. | refuse leachate is likely an important source of androgens. however, common in vitro bioassays underestimate the potential androgenic activity of leachate, owing to non-receptor-mediated mechanisms that modify the balance of sex hormones and promote the accumulation of endogenous androgens. this study aimed to develop an in vivo assay by using multiple biomarkers related to testosterone synthesis and conversion for assessing the potential androgenic activity of refuse leachate sampled from a mun ... | 2017 | 27716536 |
| the genetic map of goldfish (carassius auratus) provided insights to the divergent genome evolutions in the cyprinidae family. | a high-density linkage map of goldfish (carassius auratus) was constructed using rna-sequencing. this map consists of 50 linkage groups with 8,521 snp markers and an average resolution of 0.62 cm. approximately 84% of markers are in protein-coding genes orthologous to zebrafish proteins. we performed comparative genome analysis between zebrafish and medaka, common carp, grass carp, and goldfish to study the genome evolution events in the cyprinidae family. the comparison revealed large synteny b ... | 2016 | 27708388 |
| rapid genomic changes in allopolyploids of carassius auratus red var. (♀) × megalobrama amblycephala (♂). | to better understand genomic changes in the early generations after polyploidisation, we examined the chromosomal consequences of genomic merger in allotetraploid hybrids (4 nf1) (aabb, 4n = 148) of carassius auratus red var. (rcc) (aa, 2n = 100) (♀) × megalobrama amblycephala (bsb) (bb, 2n = 48) (♂). complete loss of the paternal 5s rdna sequence and the expected number of maternal chromosomal loci were found in 4 nf1, suggesting directional genomic changes occurred in the first generations aft ... | 2016 | 27703178 |
| identification of three selenoprotein t paralogs in goldfish (carassius auratus) and expression analysis in response to environmental stressors. | selenoproteins, which contain the selenocysteine (sec), play crucial roles in antioxidant protection. in the present study, we have identified and characterized three selenoprotein t paralogs (designated as gfselt1a, gfselt1b and gfselt2) in goldfish, carassius auratus. the secis element was found in the 3'-utrs of gfselt1a, gfselt1b and gfselt2 mrna. sequence analysis showed that they all possess the cxxu motif (where u represents sec) in the n-terminal. constitutive expressions of gfselt1a, gf ... | 2017 | 27667707 |
| the chromogranin a-derived sympathomimetic serpinin depresses myocardial performance in teleost and amphibian hearts. | chromogranin a (cga) is an acidic protein co-stored with catecholamines, hormones and neuropeptides in the secretory granules of endocrine, neuronal and other cell types (including cardiomyocytes). proteolytic cleavage in the c terminus of cga generates a 2.9kda peptide named serpinin (serp; ala26leu) that can be modified at its n terminus to form a pyroglutamate residue (pglu-serp). in the rat heart, both peptides increase contractility and relaxation through a β-adrenergic-like action mechanis ... | 2017 | 27633326 |
| complete genome sequence and architecture of crucian carp carassius auratus herpesvirus (cahv). | crucian carp carassius auratus herpesvirus (cahv) was isolated from diseased crucian carp with acute gill hemorrhages and high mortality. the cahv genome was sequenced and analyzed. the data showed that it consists of 275,348 bp and contains 150 predicted orfs. the architecture of the cahv genome differs from those of four cyprinid herpesviruses (cyhv1, cyhv2, sy-c1, cyhv3), with insertions, deletions and the absence of a terminal direct repeat. phylogenetic analysis of the dna polymerase sequen ... | 2016 | 27591780 |
| recombinant il-4/13a and il-4/13b induce arginase activity and down-regulate nitric oxide response of primary goldfish (carassius auratus l.) macrophages. | we report on the expression analysis and functional characterization of il-4/13a and il-4/13b in goldfish. quantitative analysis indicated the highest expression in the heart, spleen, brain, and kidney, with comparable expression patterns for both il-4/13a and il-4/13b. the mrna levels of il-4/13a and il-4/13b in the immune cells examined were highest in macrophage and monocytes. assessment of spleen mrna following infection with trypanosoma carassii, a prominent protozoan pathogen of fish, reve ... | 2017 | 27581741 |
| checklists of parasites of farm fishes of babylon province, iraq. | literature reviews of all references concerning the parasitic fauna of fishes in fish farms of babylon province, middle of iraq, showed that a total of 92 valid parasite species are so far known from the common carp (cyprinus carpio), the grass carp (ctenopharyngodon idella), and the silver carp (hypophthalmichthys molitrix) as well as from three freshwater fish species (carassius auratus, liza abu, and heteropneustes fossilis) which were found in some fish farms of the same province. the parasi ... | 2016 | 27559480 |
| mechanosensory lateral line nerve projections to auditory neurons in the dorsal descending octaval nucleus in the goldfish, carassius auratus. | the nucleus medialis is the main first-order target of the mechanosensory lateral line (ll) system. this report definitively demonstrates that mechanosensory ll inputs also terminate in the ipsilateral dorsal portion of the descending octaval nucleus (ddo) in the goldfish. the ddo, which is the main first-order auditory nucleus in bony fishes, includes neurons that receive direct input from the otolithic end organs of the inner ear and project to the auditory midbrain. there are two groups of su ... | 2016 | 27532270 |
| kidney activity increases in copper exposed goldfish (carassius auratus auratus). | in the present study, the effect of copper was examined in the common goldfish (carassius auratus auratus). fish were fasted and exposed to either a high (0.84μm), a low (0.34μm) or a control copper concentration (0.05μm) for 1 and 7days. swimming performance was not affected by either fasting or copper exposure. food deprivation alone had no effect on ionoregulation, but low plasma osmolality levels and plasma na(+) were noticed in fasted fish exposed to cu for 7days. both gill na(+)/k(+)-atpas ... | 2016 | 27521798 |
| acute ammonia toxicity in crucian carp carassius auratus and effects of taurine on hyperammonemia. | the four experimental groups were carried out to test the response of crucian carp carassius auratus to ammonia toxicity and taurine: group 1 was injected with nacl, group 2 was injected with ammonium acetate, group 3 was injected with ammonium acetate and taurine, and group 4 was injected with taurine. fish in group 2 had the highest ammonia and glutamine contents, and the lowest glutamate content in liver and brain. serum superoxide dismutase (sod), glutathione (gsh) activities, red cell count ... | 2016 | 27510860 |
| novel pentapeptide, palal, derived from a bony fish elicits contraction of the muscle in starfish patiria pectinifera. | a bioactive peptide mimicking peptide-signaling molecules has been isolated from the skin extract of fish channa argus which caused contraction of the apical muscle of a starfish patiria pectinifera, a deuterostomian invertebrate. the primary structure of the isolated pentapeptide comprises amino acid sequence of h-pro-ala-leu-ala-leu-oh (palal) with a molecular mass of 483.7 da. pharmacological activity of palal, dosage ranging from 10(-9) to 10(-5) m, revealed concentration-dependent contracti ... | 2016 | 27506561 |
| seawater polluted with highly concentrated polycyclic aromatic hydrocarbons suppresses osteoblastic activity in the scales of goldfish, carassius auratus. | we have developed an original in vitro bioassay using teleost scale, that has osteoclasts, osteoblasts, and bone matrix as each marker: alkaline phosphatase (alp) for osteoblasts and tartrate-resistant acid phosphatase (trap) for osteoclasts. using this scale in vitro bioassay, we examined the effects of seawater polluted with highly concentrated polycyclic aromatic hydrocarbons (pahs) and nitro-polycyclic aromatic hydrocarbons (npahs) on osteoblastic and osteoclastic activities in the present s ... | 2016 | 27498800 |
| effects of inducers of cytochrome p450s on enrofloxacin n-deethylation in crucian carp carassius auratus gibelio. | in this study with crucian carp (carassius auratus gibelio), the effect on enrofloxacin (ef) and its metabolite ciprofloxacin (cf) and on the activity of cytochrome p450 1a (cyp1a) and cytochrome p450 3a (cyp3a) was estimated following the oral administration of rifampicin (rif) (12mg/kg) and β-naphthoflavone (bnf) (12mg/kg), respectively. first, reversed-phase high-performance liquid chromatography (rp-hplc) was used to detect the pharmacokinetics of ef with continual blood sampling. in rif-tre ... | 2016 | 27490210 |
| sodium fluoride induces hypercalcemia resulting from the upregulation of both osteoblastic and osteoclastic activities in goldfish, carassius auratus. | the influence of sodium fluoride (naf) on calcium metabolism was examined in goldfish (fresh water teleost). at 2days after administration of naf (500ng/g body weight; 5μg/g body weight) (around 10(-5) to 10(-4)m in goldfish), we indicated that plasma calcium levels upregulated in both doses of naf-treated goldfish. to examine the mechanism of hypercalcemia by naf treatments, therefore, direct effects of naf on osteoblasts and osteoclasts in goldfish were investigated by an original assay system ... | 2016 | 27475026 |
| identification of nuclear localization signal within goldfish tgf2 transposase. | the structure of goldfish (carassius auratus) tgf2 transposase is still poorly understood, although it can mediate efficient gene transfer in teleost fish. we hypothesized the existence of a nuclear localization signal (nls) within tgf2 transposase to assist transport into the nucleus. to explore this, 15 consecutive amino acid residues (656-670 aa) within the c-terminus of tgf2 transposase were predicted in silico to be a nls domain. the pegfp-c1-tgf2tp(△31c) plasmid encoding the nls-domain-del ... | 2016 | 27468946 |
| in silico prediction and in vivo analysis of antiestrogenic potential of 2-isopropylthioxanthone (2-itx) in juvenile goldfish (carassius auratus). | previous studies have shown both anti-estrogenic and anti-androgenic activities of 2-isopropylthioxanthone (2-itx), a well known food contaminant, in in vitro assays. however, no data are available on the anti-estrogenic potentials and risks of 2-itx in aquatic organisms. this work evaluated the potential endocrine disrupting effects of 2-itx at the level of estrogen receptor (er) signaling cascade using juvenile goldfish (carassius auratus) as model. firstly, we investigated the ligand binding ... | 2016 | 27454205 |
| functional characterization of apoptosis-associated speck-like protein (asc) of the goldfish (carassius auratus l.). | quantitative expression analysis of goldfish asc indicated the highest and lowest mrna levels in spleen and muscle, respectively. the asc was differentially expressed in normal goldfish tissues and different immune cell populations. the highest asc mrna levels were observed in the spleen and macrophages. we generated a recombinant form of the molecule (rgfasc) and an anti-asc igg antibody, and report that treatment of goldfish macrophages with nigericin, an inducer of inflammasome pathway, up-re ... | 2016 | 27451255 |
| the oxidative stress in the liver of carassius auratus exposed to acesulfame and its uv irradiance products. | acesulfame (ace) is listed as an emerging contaminant due to its environmental persistence and wide occurrence in the environment. ace can be degraded partially in the regular uv disinfection process but the eco-toxicity of its irradiation products remains unclear. this study focused on the possible oxidative status change in the liver of carassius auratus exposed to ace and its irradiation products. the uv degradation of ace follows pseudo-first-order kinetics, and eight irradiation products we ... | 2016 | 27443459 |
| cobalt chloride treatment used to ablate the lateral line system also impairs the olfactory system in three freshwater fishes. | fishes use multimodal signals during both inter- and intra-sexual displays to convey information about their sex, reproductive state, and social status. these complex behavioral displays can include visual, auditory, olfactory, tactile, and hydrodynamic signals, and the relative role of each sensory channel in these complex multi-sensory interactions is a common focus of neuroethology. the mechanosensory lateral line system of fishes detects near-body water movements and is implicated in a varie ... | 2016 | 27416112 |
| data for amino acid alignment of japanese stingray melanocortin receptors with other gnathostome melanocortin receptor sequences, and the ligand selectivity of japanese stingray melanocortin receptors. | this article contains structure and pharmacological characteristics of melanocortin receptors (mcrs) related to research published in "characterization of melanocortin receptors from stingray dasyatis akajei, a cartilaginous fish" (takahashi et al., 2016) [1]. the amino acid sequences of the stingray, d. akajei, mc1r, mc2r, mc3r, mc4r, and mc5r were aligned with the corresponding melanocortin receptor sequences from the elephant shark, callorhinchus milii, the dogfish, squalus acanthias, the gol ... | 2016 | 27408924 |
| cryosurvival of isolated testicular cells and testicular tissue of tench tinca tinca and goldfish carassius auratus following slow-rate freezing. | experiments were carried out to test the efficiency of cryopreservation of whole testicular tissue in tench tinca tinca and goldfish carassius auratus and compare it to cryopreservation of isolated testicular cells. additionally, effects of three cryoprotectants (dimethyl sulphoxyde - me2so, methanol - meoh and ethylene glycol - eg) at three concentrations (1m, 2m and 3m) on post-thaw cell viability were assessed. tissue pieces/isolated testicular cells were diluted in cryomedia and cryopreserve ... | 2017 | 27401260 |
| toxicity and accumulation of zinc pyrithione in the liver and kidneys of carassius auratus gibelio: association with p-glycoprotein expression. | zinc pyrithione (zpt) is a broad-spectrum antibacterial and antifungal agent; therefore, it is widely used in industry and civilian life. it is discharged into the aquatic environment with industrial and civilian waste water. carassius sp. is one of the most widely distributed and farmed fish in china. the effects of aquatic zpt on carassius sp. remain unknown. in this study, we determined the acute toxicity of zpt on carassius sp. the results showed that the median lethal concentration (lc50 96 ... | 2017 | 27387320 |
| corrigendum to "tributyltin disrupts feeding and energy metabolism in the goldfish (carassius auratus)" [chemosphere 152 (2016) 221-228]. | 2016 | 27338654 | |
| the effects of galactooligosaccharide on systemic and mucosal immune response, growth performance and appetite related gene transcript in goldfish (carassius auratus gibelio). | the present study investigates the effects of supplementation of goldfish (carassius auratus gibelio) diet with galactooligosaccharide (gos) on serum immune response, mucosal immune parameters as well as appetite-related (ghrelin) and immune-related (tnf-1α and tnf-2α) genes expression. one hundred and eighty fish with an average weight of 4.88 ± 0.28 g were stocked in twelve 500-l fiberglass tank assigned to four treatments repeated in triplicates. fish were fed on experimental diets contain 0. ... | 2016 | 27311434 |
| oxidative stress responses in gills of goldfish, carassius auratus, exposed to the metribuzin-containing herbicide sencor. | metribuzin belongs to the family of asymmetrical triazine compounds and is an active ingredient in many commercial herbicides including sencor. effects on goldfish (carassius auratus l.) of exposure for 96h to 7.14, 35.7 or 71.4mgl(-1) sencor 70 wg (corresponding to 5, 25 and 50mgl(-1) of metribuzin) were examined by evaluating oxidative stress markers and activities of antioxidant and associated enzymes in gills. fish exposed to the lowest sencor concentration (7.14mgl(-1)) showed a 94% increas ... | 2016 | 27310208 |
| collective epithelial cell sheet adhesion and migration on polyelectrolyte multilayers with uniform and gradients of compliance. | polyelectrolyte multilayers (pemus) are tunable thin films that could serve as coatings for biomedical implants. pemus built layer by layer with the polyanion poly(acrylic acid) (paa) modified with a photosensitive 4-(2-hydroxyethoxy) benzophenone (paabp) group and the polycation poly(allylamine hydrochloride) (pah) are mechanically tunable by uv irradiation, which forms covalent bonds between the layers and increases pemu stiffness. pah-terminated pemus (pah-pemus) that were uncrosslinked, uv-c ... | 2016 | 27292313 |
| evidence that ghrelin may be associated with the food intake of gibel carp (carassius auratus gibelio). | ghrelin, a non-amidated peptide hormone, is a potent anorectic neuropeptide implicated in feeding regulation in mammals and non-mammalian vertebrates. however, the involvement of ghrelin in the feeding behavior of teleosts has not been well understood. to better understand the role of ghrelin in the regulation of appetite in fish, in this study, we cloned the cdnas encoding ghrelin and investigated their mrna distributions in gibel carp tissues. we also assessed the effects of different nutritio ... | 2016 | 27287038 |
| gper/gpr30, a membrane estrogen receptor, is expressed in the brain and retina of a social fish (carassius auratus) and colocalizes with isotocin. | estradiol rapidly (within 30 minutes) influences a variety of sociosexual behaviors in both mammalian and nonmammalian vertebrates, including goldfish, in which it rapidly stimulates approach responses to the visual cues of females. such rapid neuromodulatory effects are likely mediated via membrane-associated estrogen receptors; however, the localization and distribution of such receptors within the nervous system is not well described. to begin to address this gap, we identified gper/gpr30, a ... | 2017 | 27283982 |
| the satiety factor oleoylethanolamide impacts hepatic lipid and glucose metabolism in goldfish. | oleoylethanolamide (oea) is an acylethanolamide synthesized mainly in the gastrointestinal tract with known effects in mammals on food intake and body mass through activation of peroxisome proliferator-activated receptor type α (pparα). since we previously demonstrated that acute treatment with oea in goldfish resulted in decreased food intake and locomotor activity, as in mammals, we hypothesize that oea would be involved in the control of energy metabolism in fish. therefore, we assessed the e ... | 2016 | 27277972 |
| homoeologue expression insights into the basis of growth heterosis at the intersection of ploidy and hybridity in cyprinidae. | hybridization and polyploidization are considered important driving forces that form new epigenetic regulations. to study the changing patterns of expression accompanying hybridization and polyploidization, we used rna-seq and qrt-pcr to investigate global expression and homoeologue expression in diploid and tetraploid hybrids of carassius auratus red var. (♀) (r) and cyprinus carpio (♂) (c). by comparing the relative expression levels between the hybrids and their parents, we defined the expres ... | 2016 | 27265401 |
| pilot-scale uv/h2o2 advanced oxidation process for municipal reuse water: assessing micropollutant degradation and estrogenic impacts on goldfish (carassius auratus l.). | low concentrations (ng/l-μg/l) of emerging micropollutant contaminants in municipal wastewater treatment plant effluents affect the possibility to reuse these waters. many of those micropollutants elicit endocrine disrupting effects in aquatic organisms resulting in an alteration of the endocrine system. a potential candidate for tertiary municipal wastewater treatment of these micropollutants is ultraviolet (uv)/hydrogen peroxide (h2o2) as an advanced oxidation process (aop) which was currently ... | 2016 | 27262120 |
| effects of light-emitting diode spectra on the vertebrate ancient long opsin and gonadotropin hormone in the goldfish carassius auratus. | we determined the molecular mechanism underlying the environmental (photoperiodic) regulation of sexual maturation in fish, we examined the expression of sexual maturation-related hormones and vertebrate ancient long opsin (val-opsin) in goldfish (carassius auratus) exposed to different light spectra (red and green light-emitting diodes). we further evaluated the effect of exogenous gonadotropin hormone (gth) on the expression of val-opsin under different light conditions. our results demonstrat ... | 2016 | 27255995 |
| characterization of toll-like receptor gene expression in goldfish (carassius auratus) during dactylogyrus intermedius infection. | toll-like receptors (tlrs), the first and best understood innate immune receptors, play a notable role in the innate immune system by sensing pathogenic agents and initiating appropriate immune responses. however, studies about the roles of fish tlrs in response to the infection of the ectoparasitic monogenean dactylogyrus intermedius have been surprisingly vacant. in the present study, cdna fragments of five members of tlrs family in goldfish (carassius auratus) were cloned and the expression p ... | 2016 | 27238769 |
| morphological and molecular characterization of myxobolus sheyangensis n. sp. (myxosporea: myxobolidae) with intralamellar sporulation in allogynogenetic gibel carp, carassius auratus gibelio (bloch) in china. | allogynogenetic gibel carp is one of the most important freshwater cultured species in china. however, myxosporidiosis represents a severe threat to prevent the sustainable development of aquaculture of this species. during the investigation of myxosporean diversity of reared allogynogenetic gibel carp in east china, a new myxosporean with typical characteristics of myxobolus was found from 169 out of 210 (80.4 %) examined samples, designated as myxobolus sheyangensis n. sp. by combing comparati ... | 2016 | 27225002 |
| orexins and receptor ox2r in the gastroenteric apparatus of two teleostean species: dicentrarchus labrax and carassius auratus. | orexin a and b peptides and the receptor ox2r were studied in sea bass and goldfish gastroenteric tract by immunoblotting combined with densitometric analysis using nih image j software and immunohistochemical techniques. these teleost species present a different gut organization and diverse feeding habits. immunoblotting experiments showed one band of 16 kda corresponding to prepro-orexin, and one band of 38 kda corresponding to the ox2r receptor. immunohistochemical localization of oxa and oxb ... | 2016 | 27223125 |
| genetic variation and population history of three carassius auratus populations in huaihe river, china. | in order to investigate the relationships of drainage history of huaihe river with the genetic history of carassius auratus along the river, we examined the genetic variations and population histories of three wild c. auratus populations in huaihe river based on the d-loop gene. the results showed that their nucleotide and haplotype diversities were ranged from 0.00268 to 0.00651 and from 0.863 to 0.902, respectively, and their genetic distance was quite small. the analysis of molecular variance ... | 2016 | 27206839 |
| expansion of the known host range of the microsporidium, pseudoloma neurophilia. | the microsporidium, pseudoloma neurophilia, is the most common infectious organism found in laboratory zebrafish colonies. many currently used zebrafish lines originally came from pet store fish, and the initial description of p. neurophilia came from zebrafish obtained from a retail pet store. however, as p. neurophilia has not been described from wild-caught zebrafish, whether p. neurophilia is a natural pathogen of zebrafish is an open question. the pooling of fish of different species in the ... | 2016 | 27182659 |
| effect of green light spectra on the reduction of retinal damage and stress in goldfish, carassius auratus. | we investigated the effect of light spectra on retinal damage and stress in goldfish using green (530 nm) and red (620 nm) light emitting diodes (leds) at three intensities each (0.5, 1.0, and 1.5 w/m(2)). we measured the change in the levels of plasma cortisol and h2o2 and expression and levels of caspase-3. the apoptotic response of green and red led spectra was assessed using the terminal transferase dutp nick end labeling (tunel) assay. stress indicator (cortisol and h2o2) and apoptosis-rela ... | 2016 | 27181352 |
| enzyme activity and morphological change in the spleens of crucian carp in the yongcheng coal mine subsidence area, china. | the goal of the current study was to investigate the effects of pollution on aquatic organisms in the yongcheng coal mine subsidence area. crucian carp (carassius auratus) were collected from yongcheng natural fishpond (experimental group) and tianmu lake (control group), and the spleens were isolated for analysis. subsequently, histological changes, dna damage, and antioxidant enzyme activity were assessed. the result showed that there were more vacuoles, widened blood sinus cavities, increased ... | 2016 | 27173263 |
| detection of goldfish haematopoietic necrosis herpes virus (cyprinid herpesvirus-2) with multi-drug resistant aeromonas hydrophila infection in goldfish: first evidence of any viral disease outbreak in ornamental freshwater aquaculture farms in india. | this outbreak report details of a mortality event where cyprinid herpes virus-2 (cyhv-2) was detected in association with multidrug-resistant aeromonas hydrophila infection in goldfish, carassius auratus, from commercial farms. the goldfish exhibited large scale haemorrhages on the body, fins and gills, lepidorthosis, necrosed gills, protruded anus and shrunken eyes. white nodular necrotic foci in spleen and kidneys were noticed, along with necrosis and fusion of gill lamellae. transmission elec ... | 2016 | 27172876 |
| monitoring and assessment of heavy metal contamination in a constructed wetland in shaoguan (guangdong province, china): bioaccumulation of pb, zn, cu and cd in aquatic and terrestrial components. | the objective of this study is to evaluate the current status of heavy metal concentrations in constructed wetland, shaoguan (guangdong, china). sediments, three wetland plants (typha latifolia, phragmites australis, and cyperus malaccensis), and six freshwater fish species [carassius auratus (goldfish), cirrhinus molitorella (mud carp), ctenopharyngodon idellus (grass carp), cyprinus carpio (wild common carp), nicholsicypris normalis (mandarin fish), sarcocheilichthys kiangsiensis (minnows)] in ... | 2017 | 27164879 |
| ifna of triploid hybrid of gold fish and allotetraploid is an antiviral cytokine against svcv and gcrv. | triploid hybrid of gold fish (carassius auratus red var., ♀, 2n = 100) and allotetroploid (♂, 2n = 100) displays much improved disease resistance than its parents. in this paper, one of the type i ifns of triploid hybrid (3nifna) has been cloned and characterized. the full-length cdna of 3nifna consists of 740 nucleotides and the predicted 3nifna protein contains 183 amino acids. the mrna transcription of 3nifna was detected in all the selected tissues of triploid hybrid and was obviously enhanc ... | 2016 | 27157597 |
| effects of ifnγ administration on allograft rejection in ginbuna crucian carp. | in vertebrates, the rejection of allografts is primarily accomplished by cell-mediated immunity. we recently identified four ifnγ isoforms with antiviral activity in ginbuna crucian carp, carassius auratus langsdorfii. however, involvement of the ifnγ isoforms in cell-mediated immunity, especially in t cell function remains unknown. here we investigate expression of the ifnγ isoforms and effects of administration of recombinant ifnγ (rgifnγ) isoforms in ginbuna scale allograft rejection. all fou ... | 2016 | 27156851 |
| determination of estrogenic activity in the river chienti (marche region, italy) by using in vivo and in vitro bioassays. | environmental estrogen-like compounds (i.e. xenoestrogens) are a variety of pollutants, ranging from synthetic to natural occurring molecules, that are found in surface and waste waters over a wide range of concentrations. in aquatic environment, the overall estrogenic activity is often due to the presence of a mixture of chemicals and their degraded products which can induce synergistic effects. current strategies for monitoring estrogen-like chemicals are based on the use of a battery of in vi ... | 2016 | 27155408 |
| susceptibility of isogeneic ginbuna carassius auratus langsdorfii temminck et schlegel to cyprinid herpesvirus-2 (cyhv-2) as a model species. | herpesviral haematopoietic necrosis (hvhn), caused by cyprinid herpesvirus-2 (cyhv-2), has affected the commercial production of the goldfish carassius auratus and gibelio carp carassius auratus gibelio. high water temperature treatments are reported to reduce the mortality rate of infected goldfish and elicit immunity in the survivors. to define the mechanism by which this intervention induces resistance, clonal ginbuna carassius auratus langsdorfii, which is closely related to both species and ... | 2017 | 27150547 |