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introduced american eels anguilla rostrata in european waters: life-history traits in a non-native environment.this study investigated growth, condition and development of american eels anguilla rostrata that were introduced into a european river to estimate their competitive potential in a non-native habitat. results demonstrate that a. rostrata develops normally in european waters and successfully competes with the native european eel anguilla anguilla. in addition, a. rostrata appears to be more susceptible to the asian swimbladder nematode anguillicola crassus than a. anguilla and could support the f ...201424787669
presence of viruses in wild eels anguilla anguilla l, from the albufera lake (spain).a virological analysis was conducted on wild eels from the albufera lake (spain). a total of 179 individuals at different growth stages were collected in two different surveys (2004 and 2008). presence of anguillid herpesvirus (anghv-1), aquabirnavirus and betanodavirus was confirmed by pcr procedures in both surveys, although the number of detections was clearly higher in 2008 (83% of the eels analysed resulted positive for virus presence). anghv-1 was the viral agent most frequently detected, ...201424846700
speciation and demographic history of atlantic eels (anguilla anguilla and a. rostrata) revealed by mitogenome sequencing.processes leading to speciation in oceanic environments without obvious physical barriers remain poorly known. european and american eel (anguilla anguilla and a. rostrata) spawn in partial sympatry in the sargasso sea. larvae are advected by the gulf stream and other currents towards the european/north african and north american coasts, respectively. we analyzed 104 mitogenomes from the two species along with mitogenomes of other anguilla and outgroup species. we estimated divergence time betwe ...201424865601
fish-based remedies in spanish ethnomedicine: a review from a historical perspective.fish-based therapeutics is fundamentally based on a dietary use, but these vertebrates have also been employed in the treatment of infectious and parasitic diseases, during pregnancy, childbirth and postpartum and to deal with diseases of the different systems.201424885245
do north atlantic eels show parallel patterns of spatially varying selection?the two north atlantic eel species, the european and the american eel, represent an ideal system in which to study parallel selection patterns due to their sister species status and the presence of ongoing gene flow. a panel of 80 coding-gene snps previously analyzed in american eel was used to genotype european eel individuals (glass eels) from 8 sampling locations across the species distribution. we tested for single-generation signatures of spatially varying selection in european eel by searc ...201424947556
full genome sequence of a novel circo-like virus detected in an adult european eel anguilla anguilla showing signs of cauliflower disease.an adult european eel anguilla anguilla, showing typical signs of the so-called cauliflower disease, was subjected to pathological and molecular virological examinations. samples taken from internal organs and the polypoid proliferative tissue from the mouth were examined by pcr for the detection of several viruses. positive results were obtained with a nested pcr targeting the rep gene of circoviruses. analysis of the partial rep sequence indicated the presence of a putative novel circovirus, b ...201424991738
identification of molecular markers in pectoral fin to predict artificial maturation of female european eels (anguilla anguilla).the european eel is a critically endangered species that cannot be reproduced in captivity yet. artificial maturation of female european eels can be achieved via a laborious and expensive procedure, including weekly injections with pituitary extracts for up to 6 months. the success rate is highly variable and a minimally invasive method for early selection of responsive eels would prevent the unnecessary and lengthy treatment of non-responding individuals. since sexual maturation of european eel ...201424992558
sperm motility parameters and spermatozoa morphometric characterization in marine species: a study of swimmer and sessile species.the biodiversity of marine ecosystems is diverse and a high number of species coexist side by side. however, despite the fact that most of these species share a common fertilization strategy, a high variability in terms of the size, shape, and motion of spermatozoa can be found. in this study, we have analyzed both the sperm motion parameters and the spermatozoa morphometric features of two swimmer (pufferfish and european eel) and two sessile (sea urchin and ascidian) marine species. the most i ...201425016411
cucullanus egyptae sp. nov. (nematoda, cucullanidae) infecting the european eel anguilla anguilla in egypt. morphological and molecular phylogenetic studies.a total of 80 specimens of the european eel anguilla anguilla were collected during the period from february 2013 to march 2014 at the coast of the gulf of suez (red sea, egypt). a new species of parasitic nematodes was recovered and described as cucullanus egyptae. it was found in the eel's intestine with a prevalence of 68.7%. the morphology of the recovered parasite was studied by light and scanning electron microscopy. the adult worms had a wide cephalic extremity with a slit-like oral apert ...201425030116
assessment of chromosomal damage induced by a deltamethrin-based insecticide in fish (anguilla anguilla l.) - a follow-up study upon exposure and post-exposure periods.the pyrethroid insecticide decis®, containing deltamethrin as active ingredient, is among the most popular broad-spectrum biocides, with wide application in agriculture and home pest control. the occurrence of deltamethrin in the aquatic environment is well-established, but the possible genotoxic effects of decis® in non-target organisms, namely fish, remain unknown. hence, this work aimed to evaluate the cytogenetic damaging potential of decis® in european eel (anguilla anguilla l.), adopting t ...201425052525
are dna-damaging effects induced by herbicide formulations (roundup® and garlon®) in fish transient and reversible upon cessation of exposure?owing to the seasonality of crop cultivation and subsequent periodic/seasonal application of herbicides, their input to the aquatic systems is typically intermittent. consequently, exposure of fish to this type of contaminants can be short and followed by a period of permanence in non-contaminated areas. thus, the assessment of genotoxic endpoints in fish after removal of the contamination source appears as a crucial step to improve the knowledge on the dynamics of herbicide genotoxicity, as wel ...201425058560
pah metabolites in european eels (anguilla anguilla) as indicators of pah exposure: different methodological approaches.polycyclic aromatic hydrocarbons (pahs) are ubiquitous contaminants of aquatic environments derived from pyrogenic and petrogenic sources. in fish, as in other vertebrates, pahs are rapidly metabolized. however, the metabolites have been proven to induce multiple deleterious effects in fish. the concentrations of biliary polycyclic aromatic hydrocarbon metabolites in eels (anguilla anguilla) caught in polish waters were measured. the main objectives of the study were to provide information on th ...201425064716
comparison of infection success, development and swim bladder pathogenicity of two congeneric anguillicola species in experimentally infected anguilla anguilla and a. japonica.two closely related parasites, anguillicola crassus and anguillicola novaezelandiae, originally parasitizing swim bladders of the japanese eel (anguilla japonica and the short-finned eel (anguilla australis), respectively, were used for analyzing the infection success of each parasite species on either long-known, recently acquired or new definitive host species and the associated effects on the eels' swim bladders. on that account, european eels (anguilla anguilla) and japanese eels were experi ...201425070578
pcb and organochlorine pesticide burden in eels in the lower thames river (uk).thirty-five european eels (anguilla anguilla), caught in 2007 in the river thames upstream and downstream of both london and the tidal limit, were analysed for pcbs and organochlorine pesticides. most chemicals were detectable in every fish, although they have been banned or severely restricted for many years. in general, the tidal eels were more contaminated than upstream ones, which was related to their higher lipid contents. the ices7 indicator pcb concentrations ranged overall from 4.2 to 12 ...201525078785
occurrence of perfluorooctanesulfonate and perfluorooctanoic acid and histopathology in eels from north italian waters.a perfluorinated alkylated substances (pfas) biomonitoring study was conducted in european eel (anguilla anguilla) in italy for the first time. perfluorooctanesulfonate (pfos) and perfluorooctanoic acid (pfoa) concentrations were assessed in the organs of 35 wild eels from two locations, the highly impacted po river and the comacchio lagoon along the north-western adriatic coast. pfas were extracted by ion-pairing liquid extraction procedure and measured using high performance liquid chromatogra ...201525083944
effects of anguillicola novaezelandiae on the levels of cortisol and hsp70 in the european eel.the nematodes anguillicola novaezelandiae and anguillicola crassus are both alien parasites of the european eel with severe adverse effects on their new host. both species differ in terms of their invasiveness and their severity of harmful effects on the european eel. the purpose of this study was to determine under laboratory conditions whether stages of a. novaezelandiae induce stress in european eels (anguilla anguilla) and if these levels differ from stress levels induced by a. crassus. we a ...201425096532
anthropogenic noise compromises antipredator behaviour in european eels.increases in noise-generating human activities since the industrial revolution have changed the acoustic landscape of many terrestrial and aquatic ecosystems. anthropogenic noise is now recognized as a major pollutant of international concern, and recent studies have demonstrated impacts on, for instance, hearing thresholds, communication, movement and foraging in a range of species. however, consequences for survival and reproductive success are difficult to ascertain. using a series of laborat ...201525098970
mercury toxicity and the protective role of selenium in eel, anguilla anguilla.the aim of the study was to determine the impact trace metals, mainly toxic ones, on the condition of eel (anguilla anguilla) inhabiting four regions of poland. metal concentrations in eel muscle tissues were studied as functions of size, region, and season 2011-2012. the levels of metals were also used for risk assessment on consumer health. copper and zinc occurred at concentrations that could only have positive impacts on eel condition. low levels of cadmium and lead did not impair the condit ...201525099659
progression of dna damage induced by a glyphosate-based herbicide in fish (anguilla anguilla) upon exposure and post-exposure periods--insights into the mechanisms of genotoxicity and dna repair.roundup® is a glyphosate-based herbicide widely used with both agricultural and non-agricultural purposes, which has been demonstrated to represent a risk to non-target aquatic organisms, namely fish. among the described effects to fish, genotoxicity has been pointed out as one of the most hazardous. however, the genotoxic mechanisms of roundup® as well as the involvement of the oxidative dna damage repair system are not entirely understood. hence, this work aimed to improve the knowledge on the ...201425110831
the occurrence of organic contaminants in european eel (anguilla anguilla) in poland: an environmental quality assessment.the objective of the present study was to provide information on the levels of pollutants in the tissues of eels caught in polish waters. the contaminants included in the study are those which have not yet been widely studied in eel stocks, but which arouse concern in relation to the environment. an overview of the pollutant levels in eels caught in other european waters was also conducted. the results are evaluated in terms of environmental quality and consumer health. the mean concentrations o ...201425113214
a simplified method to estimate body growth parameters of the european eel anguilla anguilla.a simple approach is proposed to fit a body growth model for the european eel anguilla anguilla to data-poor case studies. the model is a modified von bertalanffy curve allowing for delayed sex determination and sexual dimorphism. the proposed procedure provides preliminary estimates of model parameters on the basis of average age and body length of silver eels.201425130672
development of techniques and technology for embryonic and larval rearing of the european eel. 201325141622
reproduction of european eel and larval culture--state of the art. 201325141740
complement activation by salivary agglutinin is secretor status dependent.after mucosal damage or gingival inflammation, complement proteins leak into the oral cavity and mix with salivary proteins such as salivary agglutinin (sag/gp-340/dmbt1). this protein is encoded by the gene deleted in malignant brain tumors 1 (dmbt1), and it aggregates bacteria, viruses and fungi, and activates the lectin pathway of the complement system. in the lectin pathway, carbohydrate structures on pathogens or altered self cells are recognized. sag is highly glycosylated, partly on the b ...201525153235
genomic footprints of speciation in atlantic eels (anguilla anguilla and a. rostrata).the importance of speciation-with-geneflow scenarios is increasingly appreciated. however, the specific processes and the resulting genomic footprints of selection are subject to much discussion. we studied the genomics of speciation between the two panmictic, sympatrically spawning sister species; european (anguilla anguilla) and american eel (a. rostrata). divergence is assumed to have initiated more than 3 ma, and although low gene flow still occurs, strong postzygotic barriers are present. r ...201425155907
abnormal ovarian dna methylation programming during gonad maturation in wild contaminated fish.there is increasing evidence that pollutants may cause diseases via epigenetic modifications. epigenetic mechanisms such as dna methylation participate in the regulation of gene transcription. surprisingly, epigenetics research is still limited in ecotoxicology. in this study, we investigated whether chronic exposure to contaminants experienced by wild female fish (anguilla anguilla) throughout their juvenile phase can affect the dna methylation status of their oocytes during gonad maturation. t ...201425203663
changes in the gene expression profiles of the brains of male european eels (anguilla anguilla) during sexual maturation.the vertebrate brain plays a critical role in the regulation of sexual maturation and reproduction by integrating environmental information with developmental and endocrine status. the european eel anguilla anguilla is an important species in which to better understand the neuroendocrine factors that control reproduction because it is an endangered species, has a complex life cycle that includes two extreme long distance migrations with both freshwater and seawater stages and because it occupies ...201425230743
interference of the co-exposure of mercury with silica-coated iron oxide nanoparticles can modulate genotoxicity induced by their individual exposures--a paradox depicted in fish under in vitro conditions.the study aimed to assess the genotoxic potential of silica-coated iron oxide nanoparticle functionalized with dithiocarbamate groups (ionp, 100 nm) in vitro exposure alone or its interference with mercury (hg) co-exposure in the blood of european eel (anguilla anguilla l.) by evaluating 8-hydroxy-2'-deoxyguanosine (8-ohdg), lipid peroxidation (lpo), and erythrocytic nuclear abnormalities (ena). four groups were made: (i) 2 × 10(6) erythrocytes + roswell park memorial institute-1640 (rpmi-1640) ...201525256583
spontaneous multicentric myxoma of the dermal nerve sheaths in farmed european eels anguilla anguilla.this report describes a peripheral nerve sheath tumour in 8 european eels anguilla anguilla l. from a fish farm located in croatia. the newborn tissue appeared as smooth and soft skin nodules without pronounced colour change. nodules were dome-shaped with a pale crater and were present on different body areas. in general, nodules were located as series of differently sized protrusions extending along the lateral line on both sides of the fish, as well as sensory canals on the head. cut sections ...201425266905
the glycoprofile patterns of endothelial cells in usual interstitial pneumonia.the pathological classification of cryptogenic fibrosing alveolitis has been a matter of debate and controversy for histopathologists.201425270010
ultrastructural characters of the spermatozoa in digeneans of the genus lecithochirium lühe, 1901 (digenea, hemiuridae), parasites of fishes: comparative study of l. microstomum and l. musculus.this study provides the first ultrastructural data of spermatozoa in the genus lecithochirium. the spermatozoa of l. microstomum (from trichiurus lepturus in senegal) and l. musculus (from anguilla anguilla in corsica) exhibit the general pattern described in the great majority of the digenea, namely two axonemes with the 9 + "1" pattern typical of the trepaxonemata, one mitochondrion, a nucleus, parallel cortical microtubules and external ornamentation of the plasma membrane. spermatozoa of l. ...201425275216
do we protect freshwater eels or do we drive them to extinction?freshwater eels are important animals because they have a unique catadromous life history and are used as food resources. european, american and japanese eel populations now are considered to be outside the safe biological limits and are seriously threatened with extinction. therefore, the european eel was recently categorised as critically endangered by the european union and the united nations. one of the reasons for the drastic decline in eel populations is overfishing, which has caused a hig ...201425279325
a century of research on the larval distributions of the atlantic eels: a re-examination of the data.the spawning areas of the atlantic freshwater eels were discovered about a century ago by the danish scientist johannes schmidt who after years of searching found newly hatched larvae of the european eel, anguilla anguilla, and the american eel, anguilla rostrata, in the southern sargasso sea. the discovery showed that anguillid eels migrate thousands of kilometers to offshore spawning areas for reproduction, and that their larvae, called leptocephali, are transported equally long distances by o ...201525291986
low larval abundance in the sargasso sea: new evidence about reduced recruitment of the atlantic eels.the european eel anguilla anguilla has shown decreased recruitment in recent decades. despite increasing efforts to establish species recovery measures, it is unclear if the decline was caused by reduced numbers of reproductive-stage silver eels reaching the spawning area, low early larval survival, or increased larval mortality during migration to recruitment areas. to determine if larval abundances in the spawning area significantly changed over the past three decades, a plankton trawl samplin ...201425307845
multiple thyrotropin β-subunit and thyrotropin receptor-related genes arose during vertebrate evolution.thyroid-stimulating hormone (tsh) is composed of a specific β subunit and an α subunit that is shared with the two pituitary gonadotropins. the three β subunits derive from a common ancestral gene through two genome duplications (1r and 2r) that took place before the radiation of vertebrates. analysis of genomic data from phylogenetically relevant species allowed us to identify an additional tshβ subunit-related gene that was generated through 2r. this gene, named tshβ2, present in cartilaginous ...201425386660
subpopulation pattern of eel spermatozoa is affected by post-activation time, hormonal treatment and the thermal regimen.there has been a marked reduction in natural stocks of eels (genus anguilla) over the past 60 years, and the culture of eels is still based on the capture of very large quantities of juveniles. it is necessary to close the life cycle in captivity in order to ease the pressure on wild populations. the aims of the present study were to evaluate sperm subpopulations (through cluster analysis of computer-aided sperm analysis data) in the european eel (anguilla anguilla) and to assess the effects of ...201525402273
nematode eel parasite found inside acanthocephalan cysts--a "trojan horse" strategy?the invasive eel parasite anguillicoloides crassus (syn. anguillicola crassus) is considered one of the major causes for the decline of the european eel (anguilla anguilla) panmictic population. it impairs the swim bladder function and reduces swimming performance of its host. the life cycle of this parasite involves different intermediate and paratenic hosts. despite an efficient immune system of the paratenic fish hosts acting against infections with a. crassus, levels of parasitized eels rema ...201425403767
the effect of drag and attachment site of external tags on swimming eels: experimental quantification and evaluation tool.telemetry studies on aquatic animals often use external tags to monitor migration patterns and help to inform conservation effort. however, external tags are known to impair swimming energetics dramatically in a variety of species, including the endangered european eel. due to their high swimming efficiency, anguilliform swimmers are very susceptibility for added drag. using an integration of swimming physiology, behaviour and kinematics, we investigated the effect of additional drag and site of ...201425409179
the potential reproductive contribution of mediterranean migrating eels to the anguilla anguilla stock.the european eel is a highly migratory fish. after the reproduction in the sargasso sea early larval-stages start a passive ocean migration towards european and mediterranean continental waters. after several years as yellow eels, mature adults change to silver stage and then start their return trip. the trajectory of their backward migration is unknown, because of low probability of capturing migrating individuals, having this capture never been reported in the mediterranean. recently, 8 silver ...201425424371
effect of photoperiod on endocrine profiles and vitellogenin expression in european eels anguilla anguilla during artificially induced ovarian development.the aim of this work was to determine the effects of dark and light conditions on the e2, testosterone and thyroid hormones levels and on the gene expression levels (vitellogenin 1, vitellogenin 2, and estradiol receptor one) in european eels (anguilla anguilla) during ovarian development induced by increasing doses of carp pituitary extracts (cpes). the subjects were divided into 2 groups: 14-hour light:10-hour dark (light group) and 24-hour darkness (dark group). all the eels received intramus ...201525459031
occurrence of pops and other persistent organic contaminants in the european eel (anguilla anguilla) from the loire estuary, france.the chemical contamination of the loire estuary by three classes of persistent organic pollutants (pops): the polychlorinated biphenyls (pcbs), the polybrominated diphenyl ethers (pbdes) and the perfluorinated and polyfluorinated alkyl substances (pfas), and three families of organic contaminants, the alkylphenols (aps), the polycyclic aromatic hydrocarbon metabolites (oh-pahs) and the bisphenol a (bpa) were investigated in the muscles and bile of european eel (anguilla anguilla). yellow eels (n ...201525461022
influence of adult anguillicoloides crassus load in european eels swimbladder on macrophage response.anguillicoloides crassus has become one of the most important threats to the european eel (anguilla anguilla). adult parasites colonize the swimbladder leading to an impaired functioning of this organ. the infection is also responsible for an increased in the stress level of infected eels, that could produce an altered immune response as well. differences in parasite loads and effects in the european and japanese eel (anguilla japonica) have been described. we have studied the influence of the n ...201525463301
transcriptome analyses of anguillicola crassus from native and novel hosts.anguillicola crassus is a swim bladder nematode of eels. the parasite is native to the asian eel anguilla japonica, but was introduced to europe and the european eel anguilla anguilla in the early 1980s. a taiwanese source has been proposed for this introduction. in the new host in the recipient area, the parasite appears to be more pathogenic. as a reason for these differences, genetically fixed differences in infectivity and development between taiwanese and european a.crassus have been descri ...201425469324
relationship between sperm quality parameters and the fatty acid composition of the muscle, liver and testis of european eel.this study looks at the correlations that fatty acids have with different tissues in the european eel (anguilla anguilla l.) during hormonally-induced sexual maturation, with different sperm quality parameters. in order to evaluate the different dynamics of the use of fatty acids, a categorization of the results from each sperm quality parameter (volume, concentration, motility and velocity) was performed. low and moderate correlations were observed between muscle tissue and some sperm quality p ...201525483240
an integrated genetic-demographic model to unravel the origin of genetic structure in european eel (anguilla anguilla l.).the evolutionary enlightened management of species with complex life cycles often requires the development of mathematical models integrating demographic and genetic data. the genetic structure of the endangered european eel (anguilla anguilla l.) has been thoroughly analyzed in several studies in the past years. however, the interpretation of the key demographic and biologic processes that determine the observed spatio-temporal genetic structure has been very challenging owing to the complex li ...201125568002
deep sequencing of the olfactory epithelium reveals specific chemosensory receptors are expressed at sexual maturity in the european eel anguilla anguilla.vertebrate genomes encode a diversity of g protein-coupled receptor (gpcr) that belong to large gene families and are used by olfactory systems to detect chemical cues found in the environment. it is not clear however, if individual receptors from these large gene families have evolved roles that are specific to certain life stages. here, we used deep sequencing to identify differentially expressed receptor transcripts in the olfactory epithelia (oe) of freshwater, seawater and sexually mature m ...201525580852
development and validation of a real-time pcr assay for the detection of anguillid herpesvirus 1.anguillid herpesvirus 1 (anghv1) causes a haemorrhagic disease with increased mortality in wild and farmed european eel, anguilla anguilla (l.) and japanese eel anguilla japonica, temminck & schlegel). detection of anghv1 is currently based on virus isolation in cell culture, antibody-based typing assays or conventional pcr. we developed, optimized and concisely validated a diagnostic taqman probe based real-time pcr assay for the detection of anghv1. the primers and probe target anghv1 open rea ...201625588414
early steps in the european eel (anguilla anguilla)-vibrio vulnificus interaction in the gills: role of the rtxa13 toxin.vibrio vulnificus is an aquatic gram-negative bacterium that causes a systemic disease in eels called warm-water vibriosis. natural disease occurs via water born infection; bacteria attach to the gills (the main portal of entry) and spread to the internal organs through the bloodstream, provoking host death by haemorrhagic septicaemia. v. vulnificus produces a toxin called rtxa13 that hypothetically interferes with the eel immune system facilitating bacterial invasion and subsequent death by sep ...201525613341
lipid peroxidation and its control in anguilla anguilla hepatocytes under silica-coated iron oxide nanoparticles (with or without mercury) exposure.having multidisciplinary applications, iron oxide nanoparticles can inevitably enter aquatic system and impact inhabitants such as fish. however, the studies in this context have ignored the significance of obvious interaction of iron oxide nanoparticles with other persistent co-contaminants such as mercury (hg) in the modulation of the toxicity and underlying mechanisms of iron oxide nanoparticles and hg alone, and concomitant exposures. this study aimed to evaluate lipid peroxidation (lpo) and ...201525613805
subcellular partitioning of non-essential trace metals (ag, as, cd, ni, pb, and tl) in livers of american (anguilla rostrata) and european (anguilla anguilla) yellow eels.we determined the intracellular compartmentalization of the trace metals ag, as, cd, ni, pb, and tl in the livers of yellow eels collected from the saint lawrence river system in canada (anguilla rostrata) and in the area of the gironde estuary in france (anguilla anguilla). differential centrifugation, naoh digestion and thermal shock were used to separate eel livers into putative "sensitive" fractions (heat-denatured proteins, mitochondria and microsomes+lysosomes) and detoxified metal fractio ...201525635611
impact of dietary fatty acids on muscle composition, liver lipids, milt composition and sperm performance in european eel.in order for european eel aquaculture to be sustainable, the life cycle should be completed in captivity. development of broodstock diets may improve the species' reproductive success in captivity, through the production of high-quality gametes. here, our aim was to evaluate the influence of dietary regime on muscle composition, and liver lipids prior to induced maturation, and the resulting sperm composition and performance. to accomplish this fish were reared on three "enhanced" diets and one ...201525638567
swimbladder function and the spawning migration of the european eel anguilla anguilla.the spawning migration of the european eel is an extensive journey over 5000 to 7000 km from the european coast to the sargasso sea. eels do not feed during this journey and on-board fuels must be sufficient to support the journey of 3.5 to 6 month, as well as sexual maturation and the spawning activity. swimming of eels appears to be quite energy efficient compared to other fish species, and elevated hydrostatic pressure has been shown to even reduce the costs of transport. recent studies revea ...201425646080
lipid increases in european eel (anguilla anguilla) in scotland 1986-2008: an assessment of physical parameters and the influence of organic pollutants.recruitment of the european eel (anguilla anguilla) has fallen steadily in recent decades, with current levels understood to be at around 5% of those in the 1970s, and the species is now widely recognised as being endangered. changes in ocean currents, climate shifts, habitat loss, overfishing, barriers to migration, increased predation, plastic litter and exposure to chemicals have all been postulated as potential causative factors. several studies have shown a general decline in eel quality (l ...201525651809
replicating phages in the epidermal mucosa of the eel (anguilla anguilla).in this work, we used the eel (anguilla anguilla) as an animal model to test the hypothesis of barr et al. (2013a,b) about the putative role of the epidermal mucosa as a phage enrichment layer. to this end, we analyzed the microbial content of the skin mucus of wild and farmed eels by using a metagenomic approach. we found a great abundance of replicating phage genomes (concatemers) in all the samples. they were assembled in four complete genomes of three myovirus and one podovirus. we also foun ...201525688234
effects of salinity and sea salt type on egg activation, fertilization, buoyancy and early embryology of european eel, anguilla anguilla.improper activation and swelling of in vitro produced eggs of european eel, anguilla anguilla, has been shown to negatively affect embryonic development and hatching. we investigated this phenomenon by examining the effects of salinity and sea salt type on egg dimensions, cell cleavage patterns and egg buoyancy. egg diameter after activation, using natural seawater adjusted to different salinities, varied among female eels, but no consistent pattern emerged. activation salinities between 30-40 p ...201625707438
the european eel may tolerate multiple infections at a low biological cost.most animals are concurrently infected with multiple parasites, and interactions among them may influence both disease dynamics and host fitness. however, the sublethal costs of parasite infections are difficult to measure and the effects of concomitant infections with multiple parasite species on individual physiology and fitness are poorly described for wild hosts. to understand the costs of co-infection, we investigated the relationships among 189 european eel (anguilla anguilla) from mar men ...201525711727
spatial and seasonal variations of methylmercury in european glass eels (anguilla anguilla) in the adour estuary (france) and relation to their migratory behaviour.overall recruitment of european glass eels (anguilla anguilla) has decreased significantly since the early 1980s. due to their long life cycle, benthic/demersal habits and high lipid content, eels might accumulate high concentrations of contaminants, but data concerning glass eels are still scarce. this study provides original data on methylmercury (mehg) concentrations in glass eels at spatial (marine and estuarine), annual and seasonal scales. the relationship between mehg concentrations in gl ...201525752638
effects of acute cooling on fish electroretinogram: a comparative study.temperature dependence of electroretinogram (erg) was investigated in 3 fish species occupying different habitats--dogfish shark (scyliorhinus canicula), prussian carp (carassius gibelio) and european eel (anguilla anguilla). acute cooling of the shark isolated eyecup from 23°c down to 6°c induced suppression of the electroretinographic b-wave--a complete degradation of this component was observed at 6°c. on the other hand, photoreceptor component of the erg, the negative late receptor potential ...201525759261
methylmercury effects on migratory behaviour in glass eels (anguilla anguilla): an experimental study using isotopic tracers.the effect of methylmercury (mehg) on glass eels' propensity to migrate, mitochondrial activity and antioxidative defence systems was investigated. marine glass eels were first sorted in an experimental flume according to their response to dusk. fish responding to the decrease in light intensity by ascending in the water column and moving with or against the flow were considered as having a high propensity to migrate (migrant). glass eels still sheltering at the end of the 24 h catching period w ...201525797033
relationship between european eel anguilla anguilla infection with non-native parasites and swimming behaviour on encountering accelerating flow.the effect of anguillicola crassus, pseudodactylogyrus bini and pseudodactylogyrus anguillae infection on the behaviour of downstream migrating adult european eels anguilla anguilla as they encountered accelerating water velocity, common at engineered structures where flow is constricted (e.g. weirs and bypass systems), was evaluated in an experimental flume. the probability of reacting to, and rejecting, the velocity gradient was positively related to a. crassus larval, adult and total abundanc ...201525801939
biochemical effects of the pharmaceutical drug paracetamol on anguilla anguilla.the ever-increasing presence of pharmaceutical drugs in the environment is a motif of concern, and human-use drugs are of particular importance. this is the case of paracetamol, a widely employed drug in human therapeutics, as analgesic and antipyretic, whose toxicity on aquatic organisms is still not fully characterized. the present study aimed to assess the toxic deleterious effects of paracetamol on european eel, anguila anguilla, by using a comprehensive battery of antioxidant biomarkers (ac ...201525827650
competing invaders: performance of two anguillicola species in lake bracciano.anguillicola crassus is one of the most successful parasitic invasive species as it has spread from its original habitat in east asia throughout the world and has acquired five new eel host species in the course of its invasion within the last three decades. records from an italian lake indicate that this species has even displaced an established population of its close relative a. novaezelandiae originating from new zealand. in order to analyze the reasons for its high invasive potential, this ...201525830111
histological and hormonal changes in the european eel (anguilla anguilla) after exposure to environmental cocaine concentration.the aim of this study was the assessment of histological and hormonal changes induced in the european eel from environmental concentrations of cocaine. silver eels were exposed to 20 ng l(-1) of cocaine during 50 days; at the same time, control, vehicle control and two post-exposure recovery groups (3 and 10 days) were made. the general morphology of the skin and the intestine, and the plasma levels of prolactin, cortisol and dopamine were evaluated. in the skin, cocaine decreased the number and ...201625865023
energy reserves mobilization in the yellow eel as herbicide exposure effect.thiobencarb and propanil are two of the most extensive used herbicides worldwide in rice cultivation. especially scanty is the available information regarding the effect of herbicides on fish energy resources. in the present study, the effect of sublethal exposure to these herbicides on the energy reserves of juvenile eel anguilla anguilla was compared. eels were exposed to 72 h to the herbicide thiobencarb (0.22 mg l(-1)) or propanil (0.63 mg l(-1)), and allowed to recover in clean water (144 h ...201525917606
duplicated leptin receptors in two species of eel bring new insights into the evolution of the leptin system in vertebrates.since its discovery in mammals as a key-hormone in reproduction and metabolism, leptin has been identified in an increasing number of tetrapods and teleosts. tetrapods possess only one leptin gene, while most teleosts possess two leptin genes, as a result of the teleost third whole genome duplication event (3r). leptin acts through a specific receptor (lepr). in the european and japanese eels, we identified two leptin genes, and for the first time in vertebrates, two lepr genes. synteny analyses ...201525946034
comparative imaging of european eels (anguilla anguilla) for the evaluation of swimbladder nematode (anguillicoloides crassus) infestation.this study compares diagnostic imaging tools in detecting the parasitic swimbladder nematode anguillicoloides crassus in anguilla anguilla (l.) and focuses on ultrasound in an attempt to develop a non-destructive, field diagnostic test. ultrasound use could allow the parasite to be diagnosed without decreasing the number of critically endangered european eels through post-mortem. in the preliminary study, eels were examined with computed radiography, computed tomography, magnetic resonance imagi ...201625952716
differences in brain gene transcription profiles advocate for an important role of cognitive function in upstream migration and water obstacles crossing in european eel.european eel is a panmictic species, whose decline has been recorded since the last 20 years. among human-induced environmental factors of decline, the impact of water dams during species migration is questioned. the main issue of this study was to pinpoint phenotypic traits that predisposed glass eels to successful passage by water barriers. the approach of the study was individual-centred and without any a priori hypothesis on traits involved in the putative obstacles selective pressure. we an ...201525962588
a global viability assessment of the european eel.the global european eel (anguilla anguilla) stock is critically endangered according to the iucn, and the european commission has urged the development of conservation plans aimed to ensure its viability. however, the complex life cycle of this panmictic species, which reproduces in the open ocean but spends most of its prereproductive life in continental waters (thus embracing a huge geographic range and a variety of habitat types), makes it difficult to assess the long-term effectiveness of co ...201525965113
trends of persistent organic pollutants in american eel (anguilla rostrata) from eastern lake ontario, canada, and their potential effects on recruitment.this study reports the history of contamination of american eels (anguilla rostrata) from eastern lake ontario (lo) by persistent organic pollutants (pops). three groups of 10 large female eels captured in eastern lo in each of 1988, 1998, and 2008 were analysed for polychlorinated dibenzo-p-dioxins and furans, polychlorinated biphenyls, several organochlorine pesticides, and polybrominated diphenyl ethers. mean concentrations were up to 3-fold lower in 2008 compared to previous years. when comb ...201526022407
local scale, coastal currents influence recruitment to freshwater populations in the european eel anguilla anguilla: a case study from the isle of man.this study examines juvenile anguilla anguilla (<30 cm) abundance in five study catchments on the isle of man. preliminary results suggest that juvenile abundance is negatively correlated with increasing coastal current speed at river mouth entry (p < 0·05). these findings indicate that at least under some circumstances, tidally driven coastal currents may influence recruitment to freshwater habitats; therefore, it is presumed that high coastal current speed at the entry to river mouths may redu ...201526033294
maternal transfer of emerging brominated and chlorinated flame retardants in european eels.the european eel (anguilla anguilla) is regarded as a critically endangered species. scientists are in agreement that the "quality of spawners" is a vital factor for the survival of the species. this quality can be impaired by parasites, disease and pollution. especially endocrine disrupting organic chemicals pose a potential threat to reproduction and development of offspring. to our knowledge, the findings in this publication for the first time describe maternal transfer of contaminants in eel ...201526042896
the effect of thawing methods on the quality of eels (anguilla anguilla).physical (colour), chemical (ph, total volatile basic nitrogen (tvb-n), thiobarbituric acid values (tba)) and microbiological (total aerobic mesophylic bacteria, salmonella, coliform, yeast and mould counts) analyses were carried out on thawed european eel (anguilla anguilla). different thawing treatments were used (in a refrigerator, in water, in air at ambient temperature and in a microwave oven). the results obtained were compared statistically with those of fresh fish. ph, tba and a(∗) value ...200826047438
transcript levels of the soluble sperm factor protein phospholipase c zeta 1 (plcζ1) increase through induced spermatogenesis in european eel.activation at fertilization of the vertebrate egg is triggered by ca(2+) waves. recent studies suggest the phospholipase c zeta (plcζ), a sperm-specific protein, triggers egg activation by an ip3-mediated ca(2+) release and allow ca(2+) waves at fertilization. in the present study we cloned, characterized, and phylogenetically positioned the european eel plcζ (plcζ1). it is 1521 bp long, with 10 exons encoding an open reading frame of 506 amino acids. the amino acid sequence contains an ef-hand ...201526051612
organophosphorus flame retardants in the european eel in flanders, belgium: occurrence, fate and human health risk.the present study investigated the levels, profiles and human health risk of organophosphorus flame retardants and plasticizers (pfrs) in wild european eels (anguilla anguilla) from freshwater bodies in the highly populated and industrial flanders region (belgium). yellow eels (n=170) were collected at 26 locations between 2000 and 2009 and for each site, muscle samples of 3-10 eels were pooled and analyzed (n=26). muscle lipid percentages varied widely between 2.4% and 21%, with a median value ...201526056994
size does matter-a closer look on anguillicola morphology.the present study deals with morphological differences between two closely related parasitic nematode species (anguillicola crassus kuwahara et al., 1974 and anguillicola novaezelandiae moravec & taraschewski, 1988) in two different experimentally infected eel species (anguilla anguilla linnaeus, 1758 and anguilla japonica temminck & schlegel, 1847). furthermore, it considers the question whether size differences between those two species are ontogenetically determined or host species-dependent. ...201526096826
pah metabolites in bile of european eel (anguilla anguilla) from morocco.environmental pollution of fish with organic contaminants is a topic of rising attention in morocco. polycyclic aromatic hydrocarbons (pah) are prominent organic contaminants which are rapidly metabolized in fish. their metabolites are accumulated in the bile fluid and can be used to assess pah exposure. the two pah metabolites 1-hydroxypyrene and 1-hydroxyphenanthrene were quantified in european eels (anguilla anguilla) from two moroccan river systems by high-performance liquid chromatography w ...201526109310
macroparasites and their communities of the european eel anguilla anguilla (linnaeus) in the czech republic.this paper summarises the results of parasitological examinations of the european eel anguilla anguilla (linnaeus) in the czech republic, carried out at the institute of parasitology, czech academy of sciences (previously the czechoslovak academy of sciences) within the period of 50 years (1958-2008). even though this survey is limited to the czech republic, it provides extensive data probably incomparable with any other study anywhere regarding the number of eels examined and parasites found. a ...201526130652
response of seaward-migrating european eel (anguilla anguilla) to manipulated flow fields.anthropogenic structures (e.g. weirs and dams) fragment river networks and restrict the movement of migratory fish. poor understanding of behavioural response to hydrodynamic cues at structures currently limits the development of effective barrier mitigation measures. this study aimed to assess the effect of flow constriction and associated flow patterns on eel behaviour during downstream migration. in a field experiment, we tracked the movements of 40 tagged adult european eels (anguilla anguil ...201526136454
influence of β-glucan leiber(®)beta-s on selected innate immunity parameters of european eel (anguilla anguilla) in an intensive farming system.nutritional support plays an important role in promoting high cellular and humoral innate immunity activity and in preventing outbreaks of disease. the effects of β-glucan leiber(®)beta-s dietary supplementation on selected nonspecific immune parameters in juvenile european eel (anguilla anguilla) in an intensive culture system were studied. the fish were fed commercial pellets containing either 0 (control group) or 200 mg leiber(®)beta-s kg-1 of feed (glucan-fed group). after four and eight wee ...201526155177
gonadal transcriptome analysis of wild contaminated female european eels during artificial gonad maturation.since the early 1980s, the population of european eels (anguilla anguilla) has dramatically declined. nowadays, the european eel is listed on the red list of threatened species (iucn red list) and is considered as critically endangered of extinction. pollution is one of the putative causes for the collapse of this species. among their possible effects, contaminants gradually accumulated in eels during their somatic growth phase (yellow eel stage) would be remobilized during their reproductive mi ...201526159298
an enriched european eel transcriptome sheds light upon host-pathogen interactions with vibrio vulnificus.infectious diseases are one of the principal bottlenecks for the european eel recovery. the aim of this study was to develop a new molecular tool to be used in host-pathogen interaction experiments in the eel. to this end, we first stimulated adult eels with different pathogen-associated molecular patterns (pamps), extracted rna from the immune-related tissues and sequenced the transcriptome. we obtained more than 2 x 10(6) reads that were assembled and annotated into 45,067 new descriptions wit ...201526207370
a physiologically based toxicokinetic (pbtk) model for moderately hydrophobic organic chemicals in the european eel (anguilla anguilla).the european eel (anguilla anguilla) is a facultatively catadromous fish species with a complex life cycle. its current population status is alarming: recruitment has decreased drastically since the 1980s and its stock is still considered to be outside safe biological limits. although there is no consensus on the reasons for this situation, it is currently thought to have resulted from a combination of different stressors, including anthropogenic contaminants. to deepen our understanding of the ...201526218567
maternally transferred dioxin-like compounds can affect the reproductive success of european eel.reported concentrations of dioxin-like compounds accumulated in the european eel (anguilla anguilla) were used to perform a risk assessment for eel larval survival, taking into account a modeled amplification of tissue concentrations with a factor of 1.33 during spawning migration. the calculated concentrations of dioxin-like compounds finally deposited in the eggs were compared with the internal effect concentrations for survival of early life stages of the european eel; these concentrations, b ...201626223357
transcriptomic profiling of male european eel (anguilla anguilla) livers at sexual maturity.the european eel anguilla anguilla has a complex life cycle that includes freshwater, seawater and morphologically distinct stages as well as two extreme long distance migrations. eels do not feed as they migrate across the atlantic to the sargasso sea but nevertheless reach sexual maturity before spawning. it is not yet clear how existing energy stores are used to reach the appropriate developmental state for reproduction. since the liver is involved in energy metabolism, protein biosynthesis a ...201526253995
changes in ovarian gene expression profiles and plasma hormone levels in maturing european eel (anguilla anguilla); biomarkers for broodstock selection.complete sexual maturation of european eels (anguilla anguilla) in captivity can only be achieved via injections with gonadotropins. for female eels this procedure takes 4-6months and the response ranges from "unresponsive" to final maturation and ovulation. reproductive success could be significantly increased via early selection of responders based on predictive markers and minimally invasive sampling methods. to get a better understanding of the genetic background of ovarian maturation of the ...201626255685
climbing experience in glass eels: a cognitive task or a matter of physical capacities?the european eel is a panmictic species, whose decline has been recorded since the last 30 years. among human-induced environmental factors of decline, the impact of water dams during species migration is questioned. indeed, water impoundments can be a severe obstacle for young eels trying to reach the upstream freshwater zones, even if they are equipped with fish-friendly passes. the passage by such devices could be an important event shaping the outcome of the future life and life history trai ...201526260433
head shape dimorphism in european glass eels (anguilla anguilla).the life cycle of the european eel (anguilla anguilla) remained a mystery until the 20th century, when schmidt discovered that the sargasso sea was its spawning area. however, many aspects of the eel's life cycle remain poorly understood. among these is the bimodal distribution in head shape, with broad- and narrowheaded phenotypes reported in the yellow eel stage. although this has been linked to dietary preferences of the yellow eels, very little is known about why, how and when this dimorphis ...201526265585
signatures of natural selection between life cycle stages separated by metamorphosis in european eel.species showing complex life cycles provide excellent opportunities to study the genetic associations between life cycle stages, as selective pressures may differ before and after metamorphosis. the european eel presents a complex life cycle with two metamorphoses, a first metamorphosis from larvae into glass eels (juvenile stage) and a second metamorphosis into silver eels (adult stage). we tested the hypothesis that different genes and gene pathways will be under selection at different life st ...201526268725
demonstration of the coexistence of duplicated lh receptors in teleosts, and their origin in ancestral actinopterygians.pituitary gonadotropins, fsh and lh, control gonad activity in vertebrates, via binding to their respective receptors, fshr and lhr, members of gpcr superfamily. until recently, it was accepted that gnathostomes possess a single fshr and a single lhr, encoded by fshr and lhcgr genes. we reinvestigated this question, focusing on vertebrate species of key-phylogenetical positions. genome analyses supported the presence of a single fshr and a single lhcgr in chondrichthyans, and in sarcopterygians ...201526271038
effect of the probiotic lactobacillus rhamnosus on the expression of genes involved in european eel spermatogenesis.positive effects of probiotics on fish reproduction have been reported in several species. in the present study, 40 male european eels were weekly treated with recombinant hcg for 9 weeks and with three different concentrations (10(3), 10(5), and 10(6) cfu/ml) of probiotic lactobacillus rhamnosus imc 501 (sinbyotec, italy). the probiotics were daily added to the water from the sixth week of the hcg treatment. males from the treated and control groups were sacrificed after 1, 2, and 3 weeks of pr ...201526271165
musculoskeletal anatomy and feeding performance of pre-feeding engyodontic larvae of the european eel (anguilla anguilla).being part of the elopomorph group of fishes, anguillidae species show a leptocephalus larval stage. however, due to largely unknown spawning locations and habitats of their earliest life stages, as well as their transparency, these anguilla larvae are rarely encountered in nature. therefore, information regarding the early life history of these larvae, including their exogenous feeding strategy and feeding performance, is rather scarce. to better understand the structural basis and functional p ...201526278932
toxic textile dyes accumulate in wild european eel anguilla anguilla.dyes are used to stain inks, paints, textile, paper, leather and household products. they are omnipresent, some are toxic and may threaten our environment, especially aquatic ecosystems. the presence of residues of sixteen dyes (triarylmethanes, xanthenes, phenothiazines and phenoxazines) and their metabolites was analyzed in muscle tissue samples of individual yellow-phased european eels (anguilla anguilla) from 91 locations in belgian rivers, canals and lakes sampled between 2000 and 2009 usin ...201526291760
chemical elements in the muscle tissues of european eel (anguilla anguilla) from selected lakes in latvia.fish is a significant source of essential nutrients, as well as toxic elements in the human diet. concentration of 17 elements was determined in muscles of eels (anguilla anguilla) collected from five fishing lakes in the territory of latvia. the concentration of main elements determined in muscle tissues varied within the following ranges: for pb, 0.019-0.047; cd, 0.0051-0.011; hg, 0.13-0.36; cu, 0.76-0.92; zn, 28-42; and as, 0.13-0.23 mg kg(-1) wet weight. a positive correlation was revealed b ...201526334326
rescheduling the process of nanoparticle removal used for water mercury remediation can increase the risk to aquatic organism: evidence of innate immune functions modulation in european eel (anguilla anguilla l.).this study aimed to assess the mechanisms of innate immune function responses to silica-coated iron oxide nanoparticle functionalized with dithiocarbamate groups (ionp) exposure alone and its associated mercury (hg) in european eel (anguilla anguilla l.) phagocytes isolated from peritoneum (p-phagocytes), gill (g-phagocytes), head kidney (hk-phagocytes) and spleen (s-phagocytes). the study evaluated viability, phagocytosis, oxidative burst activity (oba) and lipid peroxidation (lpo). four groups ...201526396010
abundance of specific mrna transcripts impacts hatching success in european eel, anguilla anguilla l.maternal mrna governs early embryonic development in fish and variation in abundance of maternal transcripts may contribute to variation in embryonic survival and hatch success in european eel, anguilla anguilla. previous studies have shown that quantities of the maternal gene products β-tubulin, insulin-like growth factor 2 (igf2), nucleoplasmin (npm2), prohibitin 2 (phb2), phosphatidylinositol glycan biosynthesis class f protein 5 (pigf5), and carnitine o-palmitoyltransferase liver isoform-lik ...201626415730
comparison of swimming capacity and energetics of migratory european eel (anguilla anguilla) and new zealand short-finned eel (a. australis).the spawning migration of the european eel (anguilla anguilla) can cover more than 6000 km, while that of the new zealand short-finned eel (a. australis) is assumed to be approximately 3000 km. since these species are expected to show adaptive traits to such an important lifetime event, we hypothesized differences in swimming capacity and energetics as a response to this adaptation. in an experimental swimming respirometer set-up, critical swimming speed (ucrit), optimal swimming speed (uopt), m ...201526441675
role of calcium on the initiation of sperm motility in the european eel.sperm from european eel males treated with hcgrec was washed in a calcium free extender, and sperm motility was activated both in the presence (seawater, sw) and in the absence of calcium (nacl+edta), and treated with calcium inhibitors or modulators. the sperm motility parameters were evaluated by a computer-assisted sperm analysis (casa) system, and changes in the [ca(2+)]i fluorescence (and in [na(+)]i in some cases) were evaluated by flow cytometry. after sperm motility was activated in a me ...201626459984
a question of origin: dioxin-like pcbs and their relevance in stock management of european eels.the stock of european eel (anguilla anguilla l.) has reached an all-time low in 2011. spawner quality of mature eels in terms of health status and fitness is considered one of the key elements for successful migration and reproduction. dioxin-like polychlorinated biphenyls (dl-pcbs) are known persistent organic pollutants potentially affecting the reproductive capability and health status of eels throughout their entire lifetime. in this study, muscle tissue samples of 192 european eels of all c ...201626477019
cutaneous myxidiosis in european eel, anguilla anguilla (linnaeus, 1758): histopathology, histochemistry and laminin immunohistochemistry.histopathology, histochemistry and immunohistochemistry of the integument of european eel, anguilla anguilla (linnaeus, 1758), infected by myxidium sp. are reported. skin samples from affected and unaffected eels were dissected, formalin fixed, paraffin embedded, sectioned and stained with h&e, periodic acid-schiff's staining method, alcian blue 8 gx ph 2.5/periodic acid-schiff's and mccallum-goodpasture's gram stain. moreover, immunohistochemistry was performed using a primary polyclonal lamini ...201626525491
influence of nematode anguillicoloides crassus infestation on the cellular and humoral innate immunity in european eel (anguilla anguilla l.).parasitic invasions are recognized as one of the primary factors responsible for decreasing populations of european eel. the aim of the present study was to determine the influence of infestation with the nematode anguillicoloides crassus on the innate immunity in european eel (anguilla anguilla). anguillicoloides crassus parasitizes the swim bladder of this fish. levels of the following immunological parameters were measured: spleen phagocyte respiratory burst activity, spleen phagocyte potenti ...201526557024
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