Publications

the influence of cryoprotectants on sturgeon (acipenser ruthenus) sperm quality, dna integrity, antioxidant responses, and resistance to oxidative stress.	this study examined the effect of cryoprotectants on dna integrity, antioxidant defense, and resistance to oxidative stress in cryopreserved sterlet acipenser ruthenus sperm. the freeze-thaw process significantly influenced sperm motility, with significant differences among cryoprotectants. in vitro exposure of cryopreserved sperm to the xanthine-xanthine oxidase (x-xo) system as a model reactive oxygen species inducer resulted in a lesser motility rate and velocity compared to the control, and ...	2015	26089085
effects of pre-incubation of eggs in fresh water and varying sperm concentration on fertilization rate in sterlet sturgeon, acipenser ruthenus.	standardization of fertilization protocols for sterlet acipenser ruthenus is crucial for improving reproductive techniques and for conservation purposes. our objectives were to determine the number of sperm (tested 430,000:1, 43,000:1, 4300:1, 430:1 sperm to egg) required to fertilize eggs and explore how pre-incubation of eggs in freshwater for 0min, 0.5min, 1min, and 10min interacts with different sperm ratios. fertilization success ranged from 29.7% at 430:1 to 84.2% at 430,000:1. pre-incubat ...	2015	26119142
novel technique for visualizing primordial germ cells in sturgeons (acipenser ruthenus, a. gueldenstaedtii, a. baerii, and huso huso).	primordial germ cells (pgcs) are the origin of all germ cells in developing embryos. in the sturgeon embryo, pgcs develop from the vegetal hemisphere, which mainly acts as an extraembryonic source of nutrition. current methods for studying sturgeon pgcs require either killing the fish or using costly and time-consuming histological procedures. here, we demonstrate that visualization of sterlet (acipenser ruthenus>) pgcs in vivo is feasible by simply labeling the vegetal hemisphere with fluoresce ...	2015	26134864
sterilization of sterlet acipenser ruthenus by using knockdown agent, antisense morpholino oligonucleotide, against dead end gene.	sturgeons (chondrostean, acipenseridae) are ancient fish species, widely known for their caviar. nowadays, most of them are critically endangered. the sterlet (acipenser ruthenus) is a common eurasian sturgeon species with a small body size and the fastest reproductive cycle among sturgeons. such species can be used as a host for surrogate production; application is of value for recovery of critically endangered and huge sturgeon species with an extremely long reproductive cycle. one prerequisit ...	2015	26248520
enzyme activity in energy supply of spermatozoon motility in two taxonomically distant fish species (sterlet acipenser ruthenus, acipenseriformes and common carp cyprinus carpio, cypriniformes).	as spermatozoon motility duration differs significantly among fish species, the mechanism of atp generation-regeneration and its distribution along the flagellum may be species-dependent. the present study compared the role of creatine kinase (ck) with that of adenylate kinase (ak) in atp regeneration during motility of demembranated spermatozoa of taxonomically distant fish species, sterlet, and common carp, allowing investigation for the presence of the creatine-phosphocreatine (pcr) shuttle i ...	2016	26483312
the antioxidant system of seminal fluid during in vitro storage of sterlet acipenser ruthenus sperm.	the role of the seminal fluid antioxidant system in protection against damage to spermatozoa during in vitro sperm storage is unclear. this study investigated the effect of in vitro storage of sterlet acipenser ruthenus spermatozoa together with seminal fluid for 36 h at 4 °c on spermatozoon motility rate and curvilinear velocity, thiobarbituric acid reactive substance level, and components of enzyme and non-enzyme antioxidant system (superoxide dismutase and catalase activity and uric acid conc ...	2016	26559692
segmental paleotetraploidy revealed in sterlet (acipenser ruthenus) genome by chromosome painting.	acipenseriformes take a basal position among actinopteri and demonstrate a striking ploidy variation among species. the sterlet (acipenser ruthenus, linnaeus, 1758; arut) is a diploid 120-chromosomal sturgeon distributed in eurasian rivers from danube to enisey. despite a high commercial value and a rapid population decline in the wild, many genomic characteristics of sterlet (as well as many other sturgeon species) have not been studied.	2015	26587056
cloning hsp70 and hsp90 genes of kaluga (huso dauricus) and the effects of temperature and salinity stress on their gene expression.	the genes encoding hsp70 and hsp90 proteins were isolated from kaluga by homologous cloning and rapid amplification of complementary dna (cdna) ends (race). hsp70 (genbank accession no. kp050541) and hsp90 (genbank accession no. kp050542) cdnas were composed of 2275 and 2718 bp and encoded polypeptides of 650 and 725 amino acids, respectively. basic local alignment search tool (blast) analysis showed that hsp70 and hsp90 of kaluga shared high identities with those of acipenser ruthenus, acipense ...	2016	26683614
effects of temperature on in vitro short-term storage of sterlet sturgeon (acipenser ruthenus) ova.	artificial propagation of sturgeons is becoming increasingly important for recovery efforts as well as for commercial production. sterlet acipenser ruthenus is a common eurasian sturgeon with a small body size and one of the fastest reproductive cycles among the sturgeons. the practical question being addressed in this study was how long fertilization of ovulated eggs can be delayed without substantially reducing the hatching rate, and an ancillary question is under what' temperature conditions ...	2016	26708725
changes of vitellogenin and lipase in captive sterlet sturgeon acipenser ruthenus females during previtellogenesis to early atresia.	plasma chemistry, lipid metabolism and vitellogenin gene expression of captive sterlet sturgeon acipenser ruthenus were studied in different maturity stages. a total of 32 fish were sampled, and maturity stages were identified on the basis of histological criteria and direct observation. females were classified to four groups: previtellogenic, vitellogenic, post-vitellogenic, and atresia. blood, gonad and liver tissue samples were taken through non-lethal biopsy. our results showed that plasma l ...	2016	26732070
cryopreservation of early stage siberian sturgeon acipenser baerii germ cells, comparison of whole tissue and dissociated cells.	several sturgeon species are near extinction; therefore an efficient conservation strategy is required. germ stem cells can be used for long-term storage and restoration of genetic information using surrogate reproduction. this study compared cryopreservation procedures of early stages of siberian sturgeon acipenser baerii testicular and ovarian cells. whole gonad tissue or dissociated cells were frozen at a cooling rate of 1 °c/min in phosphate buffered saline with 0.5% bovine serum albumin, 50 ...	2016	26920821
first report on facultative parthenogenetic activation of eggs in sterlet sturgeon, acipenser ruthenus.	this study reported facultative parthenogenetic cleavage development of sterlet sturgeon acipenser ruthenus eggs and quantified the percentage of parthenogenetically developed eggs in relation to the fertilization ability of different females. when eggs were activated in freshwater, 5.1-13.7% of eggs developed parthenogenetically, while among those activated eggs 3.6-9.4% developed to 2 cells, 0.4-4.5% developed to 4 cells, and 0-0.8% developed to 8 cells. the mean percentage of fertilized and p ...	2016	26952761
short-term storage of sterlet acipenser ruthenus testicular cells at -80 °c.	the conservation of sturgeons is of critical importance, and optimization of long-term storage is crucial to cell survival. this study aimed to examine the viability rates of several variations of sturgeon testicular cells storage at -80 °c for purpose of a short-term storage in a deep freezer or shipment on dried ice. testes extracted from three immature fish were cut into small pieces, immersed in a cryomedium composed of phosphate buffered saline with 0.5% bovine serum albumin, 50 mm glucose, ...	2016	26964775
erratum to: changes of vitellogenin and lipase in captive sterlet sturgeon acipenser ruthenus females during previtellogenesis to early atresia.		2016	27052423
[the effect of hormonal stimulation of sterlet (acipenser ruthenus l.) on steroid levels in tissue incubates].	sex steroids and corticol levels in leibovitz's l-15 media samples after incubation of intact female and male sterlet (acipenser rhutenus l.) tissue fragments and those if fishes treated with a superactive analogue of mammalian luteinising hormone-releasing hormone (lh-rh-a) were compared. 17,20β,21-trihydroxy-4-pregnen-3-one (20βs) levels were significantly higher in the media samples after incubation of ovarian follicles taken from females 5 h after treatment with lh-rh-a in comparison with 20 ...	2016	27220236
role of ca2+ in the ivm of spermatozoa from the sterlet acipenser ruthenus.	the role of ca2+ in sturgeon sperm maturation and motility was investigated. sperm from mature male sterlets (acipenser ruthenus) were collected from the wolffian duct and testis 24 h after hormone induction. testicular spermatozoa (ts) were incubated in wolffian duct seminal fluid (wdsf) for 5 min at 20°c and were designated 'ts after ivm' (tsm). sperm motility was activated in media with different ion compositions, with motility parameters analysed from standard video microscopy records. to in ...	2016	27246727
effects of feeding, digestion and fasting on the respiration and swimming capability of juvenile sterlet sturgeon (acipenser ruthenus, linnaeus 1758).	the objective of this study is to provide information on changes in swimming capability and respiration of the sterlet sturgeon (acipenser ruthenus, linnaeus 1758) caused by different levels of fasting. before testing, the four groups of sturgeon (body length: 12.1-15.4 cm, body mass: 10.0-20.2 g) fasted for 6 h, 2 days, 1 and 2 weeks, respectively. swimming tests were then performed to measure critical swimming speed and oxygen consumption at 20 ± 0.5 °c. results show: (1) fasting times shorter ...	2017	27632015
fertilization success of sterlet acipenser ruthenus and siberian sturgeon acipenser baerii gametes under conditions of heterospecific mating.	species may be prevented from interspecific hybridization by a number of different reproductive barriers that operate precopulatory and postcopulatory. in situation, when natural precopulatory reproductive barriers are affected by anthropogenic factors, postcopulatory reproductive barriers may be important for maintaining gametic isolation and hence preventing interspecific hybridization. this is highly topical in sturgeon (order acipenseriformes) which exhibits remarkable ease of interspecific ...	2016	27692499
the mammalian skeletal muscle dhpr has larger ca(2+) conductance and is phylogenetically ancient to the early ray-finned fish sterlet (acipenser ruthenus).	the l-type ca(2+) channel or dihydropyridine receptor (dhpr) in vertebrate skeletal muscle is responsible for sensing sarcolemmal depolarizations and transducing this signal to the sarcoplasmic ca(2+) release channel ryr1 via conformational coupling to initiate muscle contraction. during this excitation-contraction (ec) coupling process there is a slow ca(2+) current through the mammalian dhpr which is fully missing in euteleost fishes. in contrast to ancestral evolutionary stages where skeletal ...	2017	27793347
ultrastructural localization of intracellular calcium during spermatogenesis of sterlet (acipenser ruthenus).	calcium regulates many intracellular events such as growth and differentiation during different stages of gamete development. the aim of this study was to localize and quantify the intracellular distribution of calcium during different developmental stages of spermatogenesis in sterlet, acipenser ruthenus, using a combined oxalate-pyroantimonate technique. the distribution of calcium was described in spermatogonium, spermatocyte, spermatid, and spermatozoon stages. in the spermatogonium and sper ...	2016	27866505
gonad histology and serum 11-kt profile during the annual reproductive cycle in sterlet sturgeon adult males, acipenser ruthenus.	the aim of this study was to assess monthly testicular development in the cultured breeding stock of sterlet, acipenser ruthenus, using histological and serum sex steroid changes. testicular development in the adult male was examined monthly and showed four distinct phases including resting, pre-spawning, spawning and post-spawning. also, seasonal changes of the testes were described according to its variations in gonadosomatic index (gsi) during different phases of testicular development. using ...	2017	28109018
physiological changes and reproductive performance of sterlet sturgeon acipenser ruthenus injected with thiamine.	to evaluate the effects of thiamine on physiological changes and spawning performance of sterlet sturgeon acipenser ruthenus, 45 farmed female fish (698.6±8.9g) were randomly distributed in 9 tanks (1000l) and fed a diet with 1g/kg of an anti-thiamine drug. this was provided for 5 months prior to spawning. thiamine hydrochloride was intraperitoneally injected to fish at three different doses: 0 (t0, as control), 5 (t5) and 50 (t50) mg/kg body weight at days 30, 90 and 150 after the experiment st ...	2017	28131605
disturbances in the ploidy level in the gynogenetic sterlet acipenser ruthenus.	artificial mitotic gynogenesis, a chromosome set manipulation, is applied to provide the homozygous progeny with only maternal inheritance. here, gynogenetic development was induced in the sterlet acipenser ruthenus l. (acipenseridae) by activation of the eggs originating from albino females with the uv-irradiated spermatozoa from wild-coloured males, followed by the heat shock applied to suppress the first mitotic division in the haploid zygotes. all experimentally obtained gynogenetic offsprin ...	2017	28168627