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a reverse vaccinology approach to the identification and characterization of ctenocephalides felis candidate protective antigens for the control of cat flea infestations.despite the abundance of the domestic cat flea, ctenocephalides felis (bouché, 1835) and disease risks associated with them, flea control is difficult and requires the development of new control interventions such as vaccines. in this study, a reverse vaccinology approach was designed to achieve a rational selection of cat flea candidate protective antigens.201829347954
parasite spread at the domestic animal - wildlife interface: anthropogenic habitat use, phylogeny and body mass drive risk of cat and dog flea (ctenocephalides spp.) infestation in wild mammals.spillover of parasites at the domestic animal - wildlife interface is a pervasive threat to animal health. cat and dog fleas (ctenocephalides felis and c. canis) are among the world's most invasive and economically important ectoparasites. although both species are presumed to infest a diversity of host species across the globe, knowledge on their distributions in wildlife is poor. we built a global dataset of wild mammal host associations for cat and dog fleas, and used bayesian hierarchical mo ...201829307305
comparison of zoonotic bacterial agents in fleas collected from small mammals or host-seeking fleas from a ugandan region where plague is endemic.fleas (n = 407) were collected from small mammals trapped inside huts and surroundings of homesteads in five villages within the arua and zombo districts of uganda. the most common flea species were dinopsyllus lypusus (26%) and xenopsylla cheopis (50%). off-host fleas (n = 225) were collected inside huts by using kilonzo flea traps. the majority of the off-host fleas were ctenocephalides felis (80%). all fleas were examined for the presence of bartonella spp., rickettsia spp., and yersinia spp. ...201729276773
cat fleas (ctenocephalides felis) carrying rickettsia felis and bartonella species in hong kong.fleas are commonly recorded on stray as well as domestic dogs and cats in hong kong. fleas can be a major cause of pruritus in dogs and cats and also vectors of potentially zoonotic bacteria in the genera rickettsia and bartonella. morphological examination of 174 fleas from dogs and cats living in hong kong revealed only cat fleas (ctenocephalides felis). cytochrome c oxidase subunit 1 gene (cox1) genotyping of 20 randomly selected specimens, revealed three cox1 haplotypes (hk-h1 to hk-h3). the ...201729217417
prevalence and seasonality of fleas associated with california ground squirrels and the potential risk of tularemia in an outdoor non-human primate research facility.ectoparasites at primate research centers may be difficult to control, e.g. without exposing non-human primates (nhps) to toxicants, but their impact on nhp health is poorly understood. in 2010, there was an epizootic of tularemia at the california national primate research center (cnprc) in yolo county, california that resulted in 20 confirmed and suspect clinical cases in outdoors housed rhesus macaques (macaca mulatta [zimmermann]) and a 53% seroprevalence in the southern section of the colon ...201729202201
season-long control of flea and tick infestations in a population of cats in the aeolian archipelago using a collar containing 10% imidacloprid and 4.5% flumethrin.cats that have outdoor access are highly exposed to ticks, fleas, mites and flying insects, though the risk to become infested by arthropods is less perceived in cats than in dogs. this has resulted in fewer treatment and prevention options being available for cats than for dogs. a collar containing a combination of 10% imidacloprid and 4.5% flumethrin (seresto®, bayer animal health) is available for cats and licensed with claims against ticks and fleas for 7-8 months. following the assessment o ...201729173546
effect of naturally occurring wolbachia in anopheles gambiae s.l. mosquitoes from mali on plasmodium falciparum malaria transmission.a naturally occurring wolbachia strain (wanga-mali) was identified in mosquitoes of the anopheles gambiae complex collected in the malian villages of dangassa and kenieroba. phylogenetic analysis of the nucleotide sequence of two 16s rrna regions showed that wanga-mali clusters with wolbachia strains from supergroup a and has the highest homology to a wolbachia strain isolated from cat fleas (ctenocephalides). wanga-mali is different from two wolbachia strains previously reported in a. gambiae f ...201729114059
th1/th2 immune responses and oxidative stress in caprine flea allergy dermatitis.flea allergy dermatitis (fad) is the common, often neglected skin disease of goats caused mainly by ctenocephalides felis. this study aimed to evaluate the immuno-oxidative pathobiology of fad in goats. twelve goats from the same herd were divided into two groups of six animals each. the group i (fad) included animals with natural flea infestation and severe dermatitis lesions. the group ii (healthy control) animals were free from any parasitic infestation. to assess the pathological changes, th ...201729094355
assessment of the onset of lotilaner (credelio™) speed of kill of fleas on dogs.lotilaner (credelio™) is the newest member of the novel isoxazoline chemical class to be developed to treat canine ectoparasitism. administered orally, lotilaner is rapidly absorbed with peak blood levels occurring within 2 h post-treatment. a study was undertaken to determine the earliest onset of lotilaner's efficacy against existing flea infestations.201729089066
assessment of the speed of flea kill of lotilaner (credelio™) throughout the month following oral administration to dogs.lotilaner (credelio™, elanco), a novel isoxazoline, is a systemic insecticide and acaricide that is rapidly absorbed following oral administration to dogs and has a half-life of 30 days. as part of a development program, studies were undertaken to investigate lotilaner's initial and sustained efficacy and speed of kill against fleas.201729089019
the cat flea (ctenocephalides felis) immune deficiency signaling pathway regulates rickettsia typhi infection.rickettsia species are obligate intracellular bacteria with both conserved and lineage-specific strategies for invading and surviving within eukaryotic cells. one variable component of rickettsia biology involves arthropod vectors: for instance, typhus group rickettsiae are principally vectored by insects (i.e., lice and fleas), whereas spotted fever group rickettsiae are exclusively vectored by ticks. for flea-borne rickettsia typhi, the etiological agent of murine typhus, research on vertebrat ...201829084898
potential exposure of humans to rickettsia felis in greece.rickettsia felis is a flea-transmitted pathogen however, in greece, much work has been done on another flea-borne pathogen, r. typhi; human cases have been described and high-risk areas have been characterized. nevertheless, little is known about human infections caused by r. felis in the country since human cases are not routinely tested for antibodies against this pathogen. during the past seven years, we have set up a protocol at the national reference centre in order to improve the testing o ...201829079185
the biology and ecology of cat fleas and advancements in their pest management: a review.the cat flea ctenocephalides felis felis (bouché) is the most important ectoparasite of domestic cats and dogs worldwide. it has been two decades since the last comprehensive review concerning the biology and ecology of c. f. felis and its management. since then there have been major advances in our understanding of the diseases associated with c. f. felis and their implications for humans and their pets. two rickettsial diseases, flea-borne spotted fever and murine typhus, have been identified ...201729077073
wild rodent ectoparasites collected from northwestern iran.rodents play an important role as reservoir of some pathogens, and the host of some ectoparasites as well. these ectoparasites can transmit rodents' pathogens to human or animals. the aim of this study was to assess the distribution and infestation load of ectoparasites on rodents in meshkin-shahr district, northwestern iran.201729026851
pathogens in ectoparasites from free-ranging animals: infection with rickettsia asembonensis in ticks, and a potentially new species of dipylidium in fleas and lice.vector-borne infections are persistent public health threats worldwide. in recent years, a number of mosquito-borne viruses have emerged or re-emerged to cause major disease outbreaks. other vector-borne pathogens, however, remain understudied and much neglected especially in the developing regions of the world including southeast asia. in this study, the brown dog tick rhipicephalus sanguineus sensu lato, cat louse felicola subrostratus, and cat fleas ctenocephalides felis and ctenocephalides o ...201728969827
modelling the current distribution and predicted spread of the flea species ctenocephalides felis infesting outdoor dogs in spain.the cat flea, ctenocephalides felis, is the most prevalent flea species detected on dogs and cats in europe and other world regions. the status of flea infestation today is an evident public health concern because of their cosmopolitan distribution and the flea-borne diseases transmission. this study determines the spatial distribution of the cat flea c. felis infesting dogs in spain. using geospatial tools, models were constructed based on entomological data collected from dogs during the perio ...201728927433
first molecular detection and characterization of zoonotic bartonella species in fleas infesting domestic animals in tunisia.bartonellosis is an emerging vector-borne disease caused by different intracellular bacteria of the genus bartonella (rhizobiales: bartonellaceae) that is transmitted primarily by blood-sucking arthropods such as sandflies, ticks and fleas. in tunisia, there are no data available identifying the vectors of bartonella spp. in our research, we used molecular methods to detect and characterize bartonella species circulating in fleas collected from domestic animals in several of the country's biocli ...201728927427
bartonellosis in chronic kidney disease: an unrecognized and unsuspected diagnosis.systemic cat scratch disease or bartonellosis is a clinical entity caused by bartonella henselae, which manifests with necrotizing granulomas in visceral organs. the cat flea, ctenocephalides felis, is the vector responsible for horizontal transmission of the disease from cat to cat, and its bite can also infect humans. in immunocompromised patients including chronic kidney disease and renal transplant recipients, it can cause persistent and disseminated cat scratch disease. the aim of this pape ...201728884961
a study on prevalence and factors associated with ectoparasitism in goats of two agro-climatic regions in india.the present study was aimed to evaluate the factors associated with prevalence of various ectoparasites among goats during winter season in two different agro-climatic regions of india. a total of 320 goats (uttarakhand-208; uttar pradesh-112) were screened and evaluated for ectoparasitism and associated factors. the occurrence of ectoparasitism in goats was studied in relation with age, sex, breed, housing, agro-climatic region and system of rearing. prevalence of ectoparasitism among goats of ...201728848271
usefulness of a topical combination of dinotefuran and pyriproxyfen for long-term control of clinical signs of allergic dermatitis in privately-owned cats in ile-de-france region.the present study assessed the activity of a combination of dinotefuran and pyriproxyfen (vectra® felis) for long-term control (3 months) of allergic dermatitis (ad) in privately-owned cats under common household conditions in ile-de-france region.201728830567
efficacy and safety of sarolaner (simparica®) in the treatment and control of naturally occurring flea infestations in dogs presented as veterinary patients in australia.the efficacy and safety of a novel isoxazoline compound, sarolaner (simparica®, zoetis) and spinosad (comfortis®, elanco) as a positive control were evaluated for the treatment and control of natural flea infestations on dogs in two randomised, blinded, multi-centric clinical trials conducted in 11 veterinary clinics in northeastern and southeastern states of australia.201728814332
evaluation of sarolaner and spinosad oral treatments to eliminate fleas, reduce dermatologic lesions and minimize pruritus in naturally infested dogs in west central florida, usa.an in-home investigation of naturally flea infested dogs was conducted in west central florida, usa to evaluate and compare the effectiveness of two different oral flea adulticides to control flea infestations, minimize dermatologic lesions and reduce pruritus over an 8-week period.201728814316
the sustained speed of kill of ticks (rhipicephalus sanguineus) and fleas (ctenocephalides felis felis) on dogs by a spot-on combination of fipronil and permethrin (effitix®) compared with oral afoxolaner (nexgard®).the rapid speed of kill of a spot-on, combination of fipronil-permethrin (effitix®, virbac) was shown against infestations of rhipicephalus sanguineus and ctenocephalides felis on dogs. efficacy was determined against new infestations at weekly intervals for one month after treatment. dogs were allocated randomly to either an untreated control or to a single administration, given on day 0, of either topical fipronil-permethrin (6.7-13.4mg/kg and 60-120mg/kg, respectively) or oral afoxolaner (2.7 ...201728807310
gastrointestinal helminths and ectoparasites in the stray cats (felidae: felis catus) of ahar municipality, northwestern iran.the stray cats are considered as the sources of emerging humans and domestic livestock pathogens and the zoonoses of public health importance. the present study was aimed to elucidate intestinal helminth infections and infestation with ectoparasites of the stray cats of ahar city, northwestern iran.201728761492
synergy between dinotefuran and fipronil against the cat flea (ctenocephalides felis): improved onset of action and residual speed of kill in adult cats.the cat flea, ctenocephalides felis felis (c. felis), is a cosmopolitan hematophagous ectoparasite, and is considered to be the most prevalent flea species in both europe and the usa. clinical signs frequently associated with flea bites include pruritus, dermatitis and in severe cases even pyodermatitis and alopecia. ctenocephalides felis is also a vector for several pathogens and is an intermediate host for the cestode dipylidium caninum. treatment of cats with a fast-acting pulicide, that is p ...201728724438
comparative analysis of storage conditions and homogenization methods for tick and flea species for identification by maldi-tof ms.ticks and fleas are vectors for numerous human and animal pathogens. controlling them, which is important in combating such diseases, requires accurate identification, to distinguish between vector and non-vector species. recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) was applied to the rapid identification of arthropods. the growth of this promising tool, however, requires guidelines to be established. to this end, standardization protocols ...201728722283
cat scratch disease: u.s. clinicians' experience and knowledge.cat scratch disease (csd) is a zoonotic infection caused primarily by the bacterium bartonella henselae. an estimated 12,000 outpatients and 500 inpatients are diagnosed with csd annually, yet little is known regarding clinician experience with and treatment of csd in the united states. questions assessing clinical burden, treatment and prevention of csd were posed to 3,011 primary care providers (family practitioners, internists, paediatricians and nurse practitioners) during 2014-2015 as part ...201728707827
direct evidence of rickettsia typhi infection in rhipicephalus sanguineus ticks and their canine hosts.murine typhus is a rickettsiosis caused by rickettsia typhi, whose transmission is carried out by rat fleas in urban settlements as classically known, but it also has been related to cat fleas in a sub-urban alternative cycle that has been suggested by recent reports. these studies remarks that in addition to rats, other animals like cats, opossums and dogs could be implied in the transmission of rickettsia typhi as infected fleas obtained from serologically positive animals have been detected i ...201728652984
[epidemiological and molecular profile of rickettsiosis in peruvian border locations].to determine the circulation of rickettsia in the years 2010 and 2011 in border locations in four regions ofperu and their clinical epidemiological and molecular characteristics.201728538849
first description of bartonella koehlerae infection in a spanish dog with infective endocarditis.bartonella koehlerae has been recently described as a new cat- and cat fleas-associated agent of culture-negative human endocarditis. it has been also encountered in one dog from israel and six dogs from the usa, but other clinically relevant reports involving this bacterium are lacking.201728526098
preventive efficacy of nexgard spectra(®) against dipylidium caninum infection in dogs using a natural flea (ctenocephalides felis) infestation model.the efficacy of a monthly oral endectocide product, nexgard spectra(®) (merial), a combination of afoxolaner and milbemycin oxime, was evaluated in a flea (ctenocephalides felis) challenge model for the prevention of dipylidium caninum tapeworm infection in dogs. the efficacy of treatment with nexgard spectra(®) was assessed in 10 dogs following weekly flea infestation with metacestode naturally infected fleas and compared with that in 10 untreated control dogs. the 100 fleas deposited weekly on ...201728497745
a field trial of a fixed combination of permethrin and fipronil (effitix®) for the treatment and prevention of flea infestation in dogs living with sheep.a large number of fleas parasitize dogs living with sheep in greece. the primary aim of this randomized, blinded, placebo-controlled trial was to examine the efficacy of a permethrin-fipronil combination (effitix®) for the treatment and prevention of flea infestation in dogs living with sheep and the secondary aim was to examine the efficacy of this intervention on flea infestation, pruritus and skin lesions of the people in contact with these dogs.201728454576
first report of a rickettsia asembonensis related infecting fleas in brazil.the present study was performed in a non-endemic area for spotted fever (sf) in imperatriz microregion, state of maranhão, brazil. blood samples and ectoparasites were collected from 300 dogs of the imperatriz microregion. canine serum samples were tested individually by indirect immunofluorescence assay (ifa), using five rickettsia isolates from brazil. antibodies reactive to at least one of the five species of rickettsia were detected in 1.6% of the dogs (5/300). these sera were considered rea ...201728427962
spotted fever: epidemiology and vector-rickettsia-host relationship in rio de janeiro state.the eco-epidemiological scenario of spotted fever (sf), a tick-borne disease that affects humans and other animals in several countries around the world, was analyzed in rio de janeiro (rj) state, brazil. during the last 34 years, 990 sf cases were reported in rj (the brazilian state with the highest population density), including 116 cases confirmed by serology (rifi) or pcr, among 42.39% of the municipalities with reported cases of sf. the epidemiologic dynamics of sf in rj state are very hete ...201728424664
high prevalence of rickettsia spp. in dog fleas (siphonaptera: pulicidae) in rural uganda.fleas are known vectors of zoonotic agents. thirty-five fleas, including 28 ctenocephalides felis (bouché), four pulex irritans (l.), and three echidnophaga gallinacea (westwood) from 19 rural dogs from southwestern uganda were analyzed for the presence of rickettsia spp. (ompb, glta, and 17 kda fragment genes) and bartonella spp. (rpob and its genes) by pcr. rickettsial dna was detected in 27 out of 28 of ct. felis and in two out of four p. irritans. none of the e. gallinacea specimens harbored ...201728399271
effect of rickettsia felis strain variation on infection, transmission, and fitness in the cat flea (siphonaptera: pulicidae).rickettsia felis is a human pathogen transmitted by the cat flea, ctenocephalides felis (bouché) (str. lsu), as well as an obligate symbiont of the parthenogenic booklouse liposcelis bostrychophila (badonnel) (str. lsu-lb). the influence of genetic variability in these two strains of r. felis on host specialization and fitness and possible resulting differences on infection and transmission kinetics in c. felis is unknown. utilizing an artificial host system, cat fleas were exposed to a r. felis ...201728399259
first detection of rickettsia typhi and rickettsia felis in fleas collected from client-owned companion animals in the southern great plains.flea-borne rickettsiosis occurs worldwide and includes a number of pathogens, namely, rickettsia typhi and rickettsia felis. most studies in the united states have occurred in southern texas and california where flea-borne rickettsiosis is endemic, resulting in a lack of information from other regions of the country. between march and august 2016, 222 fleas were collected from 52 client-owned dogs and cats in two urban areas in oklahoma. fleas were identified using morphological characteristics ...201728399210
efficacy of a new spot-on formulation of selamectin plus sarolaner for cats against adult ctenocephalides felis, flea egg production and adult flea emergence.a new spot-on formulation of selamectin plus sarolaner was evaluated against fleas for adulticidal efficacy, and for the effect on egg production and hatching when applied to flea-infested cats. ten male and ten female adult domestic shorthair cats were randomly assigned to one of two treatment groups based on pre-treatment flea counts. cats received topical treatment on day 0 in a single spot to the dorsal scapular area with either a placebo formulation or with the combination formulation at th ...201728395752
efficacy and speed of kill of a new spot-on formulation of selamectin plus sarolaner against flea infestations in cats.the efficacy of a new spot-on formulation of selamectin plus sarolaner against induced flea infestations in cats was confirmed in three placebo-controlled, blinded studies. purpose-bred adult cats (n=8/group) were blocked by pre-treatment flea counts and randomly allocated to treatment with either a placebo or with the spot-on formulation at the minimum dose of 6.0mg selamectin and 1.0mg sarolaner per kg bodyweight. treatments were applied topically once on day 0. all cats were infested with app ...201728395751
prevalence of ectoparasites in dogs of shimoga, karnataka.a study was carried out to ascertain the status of ecto-parasites infesting dogs of shimoga region. a total of 120 dogs with the history of skin problems presented to the local hospitals and local pet clinics of shimoga region were screened for different ecto-parasites. the ticks, fleas and lice were collected, processed and identified. the skin scrapings were also collected from the affected dogs and processed for identification of mites. out of 120 dogs examined, 59 (49.1 %) had harboured ecto ...201728316406
feline and canine leishmaniosis and other vector-borne diseases in the aeolian islands: pathogen and vector circulation in a confined environment.vector-borne diseases (vbds) are prevalently investigated in dogs. studies on feline vbds are scant, though feline leishmaniosis (fel) is increasingly recognised as a disease of cats in endemic areas. comprehensive investigations on the distribution of vbds in populations of cats and dogs living in relatively small geographical areas, such as islands, are currently lacking. in this study the prevalence of leishmania infantum and other vbd pathogens was assessed in cohorts of cats and dogs living ...201728288759
diversity of rickettsiae in a rural community in northern california.far northern california forests are highly biodiverse in wildlife reservoirs and arthropod vectors that may propagate rickettsial pathogens in nature. the proximity of small rural communities to these forests puts people and domestic animals at risk of vector-borne infection due to spillover from wildlife. the current study was conducted to document exposure to rickettsial pathogens in people and domestic animals in a rural community, and identify which rickettsiae are present in sylvatic and pe ...201728274808
bartonella species pathogenic for humans infect pets, free-ranging wild mammals and their ectoparasites in the caatinga biome, northeastern brazil: a serological and molecular study.this study verified the occurrence of bartonella spp. in dogs, cats, wild mammals and their ectoparasites in petrolina and lagoa grande counties, pernambuco, located in a semi-arid region in northeastern brazil. anti-bartonella spp. antibodies were detected by indirect immunofluorescence assay (ifa) in 24.8% of dogs (27/109) and in 15% of cats (6/40). bartonella sp. dna was identified by pcr performed on dna extracted from blood and ectoparasites using primers targeting bartonella sp. glta and r ...201728249707
cat fleas (ctenocephalides felis) from cats and dogs in new zealand: molecular characterisation, presence of rickettsia felis and bartonella clarridgeiae and comparison with australia.the cat flea (ctenocephalides felis) is the most common flea species parasitising both domestic cats and dogs globally. fleas are known vectors of zoonotic pathogens such as vector borne rickettsia and bartonella. this study compared cat fleas from domestic cats and dogs in new zealand's north and south islands to australian cat fleas, using the mitochondrial dna (mtdna) marker, cytochrome c oxidase subunit i and ii (cox1, cox2). we assessed the prevalence of rickettsia and bartonella using genu ...201728115179
limited evidence for rickettsia felis as a cause of zoonotic flea-borne rickettsiosis in southern california.over 90% of human flea-borne rickettsioses cases in california are reported from suburban communities of los angeles and orange counties and are presumed to be associated with either rickettsia typhi or rickettsia felis infection. ctenocephalides felis (bouché) is considered the principal vector for both rickettsiae, and r. felis has largely replaced r. typhi as the presumptive etiologic agent based on the widespread incidence of r. felis in cat flea populations. however, with no evidence to con ...201728082625
gregarine infection accelerates larval development of the cat flea ctenocephalides felis (bouché).a high degree of specialization between host and parasite is a well-known outcome of a long history of coevolution, and it is strikingly illustrated in a coordination of their life cycles. in some cases, the arms race ensued at the establishment of a symbiotic relationship results in the adoption of manipulative strategies by the parasite. we have already learned that steinina ctenocephali, a gregarine living in the alimentary canal of cat flea, ctenocephalides felis follows its phenology and me ...201728073394
initial evaluations of the effectiveness of spinetoram as a long-acting, oral systemic pulicide for controlling cat flea (ctenocephalides felis) infestations on dogs.spinetoram is a semi-synthetic, spinosyn class natural product derived from fermentation by the actinomycete, saccharopolyspora spinosa. based on ld50 (50% lethal dose) values against adult cat fleas (ctenocephalides felis) using an in vitro contact assay, spinetoram was approximately 4-fold more potent than spinosad. subsequently, two parallel-arm, randomized block design laboratory studies were conducted to evaluate the effectiveness of orally administered spinetoram against experimental c. fe ...201728043384
a molecular survey for francisella tularensis and rickettsia spp. in haemaphysalis leporispalustris (acari: ixodidae) in northern california.francisella tularensis and rickettsia spp. have been cultured from haemaphysalis leporispalustris packard, but their prevalence in this tick has not been determined using modern molecular methods. we collected h. leporispalustris by flagging vegetation and leaf litter and from lagomorphs (lepus californicus gray and sylvilagus bachmani (waterhouse)) in northern california. francisella tularensis dna was not detected in any of 1,030 ticks tested by polymerase chain reaction (pcr), whereas 0.4% of ...201628031350
intrinsic activity of igrs against larval cat fleas.insect growth regulators (igrs) such as lufenuron, methoprene, and pyriproxyfen have been important tools in the integrated pest management of cat fleas, ctenocephalides felis (bouché), for the past two decades. other igrs have been registered for the control of termite, dipteran, and lepidopterous pests including chlorfluazuron, cyromazine, dicyclanil, and precocene i, but have been not tested against c. felis. the intrinsic activity of igrs was determined by exposing larvae to treated larval r ...201728011733
papular urticaria: a review of causal agents in colombia.papular urticaria is a chronic allergic reaction induced by insect bites, which is common in the tropics. the objective of this review was to deepen on epidemiological and immunological aspects of this disease, focused on data published in latin american countries.we conducted a non-systematic review of the literature through electronic search on the epidemiology of papular urticaria, the entomological characteristics of the causative agents and associated immunological mechanisms.several report ...201627992990
assessment of the efficacy of a topical combination of fipronil-permethrin (frontline tri-act(®)/frontect(®)) against egg laying and adult emergence of the cat flea (ctenocephalides felis) in dogs.this study was conducted to assess the prevention of egg laying and the inhibition of the emergence of the cat flea (ctenocephalides felis) resulting from the application of a combination of fipronil and permethrin (frontline tri-act(®)/frontect(®), merial) on dogs. sixteen healthy dogs were included after pre-treatment live flea counts and randomly allocated to two groups. eight dogs served as untreated controls and 8 dogs were treated on day 0 and day 30 with topical application of fipronil/pe ...201627991415
pet-related infections.physicians and veterinarians have many opportunities to partner in promoting the well-being of people and their pets, especially by addressing zoonotic diseases that may be transmitted between a pet and a human family member. common cutaneous pet-acquired zoonoses are dermatophytosis (ringworm) and sarcoptic mange (scabies), which are both readily treated. toxoplasmosis can be acquired from exposure to cat feces, but appropriate hygienic measures can minimize the risk to pregnant women. persons ...201627929279
does hair coat length affect flea infestation in naturally infested dogs?the siphonaptera are parasitic insects of endothermic animals and ctenocephalides felis and pulex irritans are important parasites of dogs. this study evaluated the effect of hair coat length and time of year on the population size of c. felis and p. irritans in naturally infested dogs. fleas were collected from 14 dogs on a monthly basis for a year (february 2015 to january 2016) at a rural property in bagé, rio grande do sul, brazil. the dogs were divided into two groups based on hair coat len ...201627925064
limited evidence for rickettsia felis as a cause of zoonotic flea-borne rickettsiosis in southern california.over 90% of human flea-borne rickettsioses cases in california are reported from suburban communities of los angeles and orange counties and are presumed to be associated with either rickettsia typhi or rickettsia felis infection. ctenocephalides felis (bouché) is considered the principal vector for both rickettsiae, and r. felis has largely replaced r. typhi as the presumptive etiologic agent based on the widespread incidence of r. felis in cat flea populations. however, with no evidence to con ...201627816916
parasites and vector-borne diseases in client-owned dogs in albania: infestation with arthropod ectoparasites.to establish the diversity and seasonality of ectoparasite infestation in client-owned dogs in albania, 602 dogs visiting four small animal clinics in tirana from march 2010 to april 2011 inclusive were examined for ectoparasites by full body search and total body comb. in addition, ear swab specimens collected from all dogs and scrapings taken from skin lesions suspicious of mite infestation were examined for parasitic mites. overall, 93 dogs (15.4 %, 95%ci 12.6-18.6) were demonstrated to be in ...201727796564
flea species infesting dogs in spain: updated spatial and seasonal distribution patterns.this entomological survey examines the spatial and seasonal distribution patterns of flea species infesting dogs in spain. bioclimatic zones covering broad climate and vegetation ranges were surveyed according to size. in a cross-sectional spatial survey carried out from late may 2013 to mid-july 2015, 1084 dogs from 42 different locations were examined. a total of 3032 fleas were collected and identified as belonging to the following species: ctenocephalides felis (siphonaptera: pulicidae) (81. ...201727790728
ectoparasites of free-roaming domestic cats in the central united states.free-roaming domestic cat (felis catus) populations serve as a valuable resource for studying ectoparasite prevalence. while they share a similar environment as owned cats, free-roaming cats do not receive routine veterinary care or ectoparasiticide application, giving insight into parasite risks for owned animals. we examined up to 673 infested cats presented to a trap-neuter-return (tnr) clinic in the central united states. ectoparasite prevalences on cats were as follows: fleas (71.6%), ticks ...201627692321
evaluation of pharmacokinetics and efficacy of ivermectin following oral administration in dogs against experimental infection of ctenocephalides felis felis and rhipicephalus sanguineus.with the increasing number of pets in home the human-animal relationship is increasingly close and care about control disease growing. ivermectin (ivm) is frequently used because its proven safety. ivm is recommended for the treatment of demodectic scabies and prevention of heartworm in dogs, but informally is extremely used to control of ctenocephalides felis felis and rhipicephalus sanguineus. the aim of this study is evaluate the use of ivm in dogs, by the oral route at 0.6μg/kg dose, against ...201627692320
differential rickettsial transcription in bloodfeeding and non-bloodfeeding arthropod hosts.crucial factors influencing the epidemiology of rickettsia felis rickettsiosis include pathogenesis and transmission. detection of r. felis dna in a number of arthropod species has been reported, with characterized isolates, r. felis strain lsu and strain lsu-lb, generated from the cat flea, ctenocephalides felis, and the non-hematophagous booklouse, liposcelis bostrychophila, respectively. while it is realized that strain influence on host biology varies, the rickettsial response to these disti ...201627662479
seroepidemiology of rickettsial infections in northeast india.resurgence of scrub typhus was reported in northeast india in 2010 after a gap of 67 years since world war ii. however, the presence of other rickettsial infections remained unknown from this region. a seroepidemiological investigation was undertaken in the scrub typhus affected areas from 2013-2015 in assam, arunachal pradesh and nagaland to assess the exposure to other rickettsial diseases besides scrub typhus.201627618917
development of a larval bioassay method using 96-well microtiter plates for evaluation of susceptibility of the cat fleas (siphonaptera: pulicidae) to insecticides. 201727614898
detection of rickettsiae in fleas and ticks from areas of costa rica with history of spotted fever group rickettsioses.outbreaks of spotted fevers have been reported in costa rica since the 1950s, although vectors responsible for transmission to humans have not been directly identified. in this study, species of rickettsia were detected in ectoparasites from costa rica, mostly from five study sites where cases of spotted fevers have been reported. ticks and fleas were collected using drag cloths or directly from domestic and wild animals and pooled according to species, host, and location. pools were analyzed in ...201627592065
fleas and ticks in carnivores from a domestic-wildlife interface: implications for public health and wildlife.fleas and ticks are parasites of wild and domestic mammals, and can be vectors of several pathogens. in rural areas, domestic carnivores such as the domestic dog (canis lupus familiaris l.), may act as a "bridge" between natural areas and human settlements where ectoparasites can be used as a metric of such link. the aim of this study was to identify fleas, ticks, and rickettsia spp., collected from domestic and wild carnivores in a natural reserve and surrounding human settlements in central ch ...201627539151
rickettsial infections among ctenocephalides felis and host animals during a flea-borne rickettsioses outbreak in orange county, california.due to a resurgence of flea-borne rickettsioses in orange county, california, we investigated the etiologies of rickettsial infections of ctenocephalides felis, the predominant fleas species obtained from opossums (didelphis virginiana) and domestic cats (felis catus), collected from case exposure sites and other areas in orange county. in addition, we assessed the prevalence of igg antibodies against spotted fever group (sfgr) and typhus group (tgr) rickettsiae in opossum sera. of the 597 flea ...201627537367
infection rates of wolbachia sp. and bartonella sp. in different populations of fleas.in the present study, a molecular detection of bartonella sp. and wolbachia sp. in ctenocephalides felis (siphonaptera: pulicidae) isolated from canis lupus familiaris from different geographical areas of spain, iran and south africa, and in stenoponia tripectinata tripectinata isolated from mus musculus from the canary islands has been carried out by amplification of the 16s ribosomal rna partial gene of wolbachia sp. and intergenic spacer region (its region) of bartonella sp. a total of 70 % o ...201627515668
isolation and characterization of a novel rickettsia species (rickettsia asembonensis sp. nov.) obtained from cat fleas (ctenocephalides felis).a novel rickettsial agent, 'candidatus rickettsia asembonensis' strain nmrciit, was isolated from cat fleas, ctenocephalides felis, from kenya. genotypic characterization of the new isolate based on sequence analysis of five rickettsial genes, rrs, glta, ompa, ompb and sca4, indicated that this isolate clustered with rickettsia felis urrwxcal2. the degree of nucleotide similarity demonstrated that isolate nmrciit belongs within the genus rickettsia and fulfils the criteria for classification as ...201627506201
rickettsial disease in the peruvian amazon basin.using a large, passive, clinic-based surveillance program in iquitos, peru, we characterized the prevalence of rickettsial infections among undifferentiated febrile cases and obtained evidence of pathogen transmission in potential domestic reservoir contacts and their ectoparasites. blood specimens from humans and animals were assayed for spotted fever group rickettsiae (sfgr) and typhus group rickettsiae (tgr) by elisa and/or pcr; ectoparasites were screened by pcr. logistic regression was used ...201627416029
reference gene selection and rna preservation protocol in the cat flea, ctenocephalides felis, for gene expression studies.the cat flea, ctenocephalides felis, is a major pest species on companion animals thus of significant importance to the animal health industry. the aim of this study was to develop sampling and storage protocols and identify stable reference genes for gene expression studies to fully utilize the growing body of molecular knowledge of c. felis. rna integrity was assessed in adult and larvae samples, which were either pierced or not pierced and stored in rnalater at ambient temperature. rna qualit ...201627406059
evaluation of fluralaner and afoxolaner treatments to control flea populations, reduce pruritus and minimize dermatologic lesions in naturally infested dogs in private residences in west central florida usa.a study was conducted to evaluate and compare the effectiveness of two different oral flea and tick products to control flea infestations, reduce pruritus and minimize dermatologic lesions over a 12 week period on naturally infested dogs in west central fl usa.201627352607
rickettsia felis, an emerging flea-borne rickettsiosis.rickettsia felis is an emerging insect-borne rickettsial pathogen and the causative agent of flea-borne spotted fever. first described as a human pathogen from the usa in 1991, r. felis is now identified throughout the world and considered a common cause of fever in africa. the cosmopolitan distribution of this pathogen is credited to the equally widespread occurrence of cat fleas (ctenocephalides felis), the primary vector and reservoir of r. felis. although r. felis is a relatively new member ...201627340613
opossums and cat fleas: new insights in the ecology of murine typhus in galveston, texas.murine typhus is an acute undifferentiated febrile illness caused by rickettsia typhi the classic reservoir (rattus spp.) and flea vector (xenopsylla cheopis) were once culprits of murine typhus in the united states. vector and rodent control efforts have drastically decreased the prevalence of disease, except in a few endemic foci where opossums and cat fleas play a role in transmission. since 2012, there has been a reemergence of murine typhus in galveston, tx. we hypothesize that opossums and ...201627273642
the effect of water and shampooing on the efficacy of fluralaner spot-on solution against ixodes ricinus and ctenocephalides felis infestations in dogs.fluralaner spot-on solution provides immediate and persistent efficacy against tick and flea infestations in dogs and cats for 12-weeks following topical administration. the active ingredient fluralaner is distributed systemically following transdermal absorption. therefore, this study tested the hypothesis whether water-immersion or shampooing of dogs following administration of fluralaner spot-on solution has an impact on subsequent tick and flea efficacy.201627241021
molecular identification and phylogenic analysis of bartonella henselae isolated from iranian cats based on glta gene.one of the most important species of the bartonella genus is b. henselae that causes a zoonotic infection, cat scratch disease (csd). the main source of the bacteria is cat and the carrier is ctenocephalides felis flea. one hundred and forty nail and saliva samples were collected from 70 domestic cats. positive samples for b. henselae were characterized by polymerase chain reaction (pcr) and sequencing. sequences of glta gene were trimmed using bioedit software and then compared with the sequenc ...201627226890
optimization of isoxazoline amide benzoxaboroles for identification of a development candidate as an oral long acting animal ectoparasiticide.novel isoxazoline amide benzoxaboroles were designed and synthesized to optimize the ectoparasiticide activity of this chemistry series against ticks and fleas. the study identified an orally bioavailable molecule, (s)-n-((1-hydroxy-3,3-dimethyl-1,3-dihydrobenzo[c][1,2]oxaborol-6-yl)methyl)-2-methyl-4-(5-(3,4,5-trichlorophenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazol-3-yl)benzamide (23), with a favorable pharmacodynamics profile in dogs (cmax=7.42ng/ml; tmax=26.0h; terminal half-life t1/2=127h). ...201627210432
efficacy of a novel topical combination of fipronil 9.8% and (s)-methoprene 8.8% against ticks and fleas in naturally infested dogs.the efficacy of a novel topical combination of fipronil 9.8% (w/v) and (s)-methoprene 8.8% (w/v) (fiprofort® plus) was tested against ticks and fleas in naturally infested dogs. a total of fifty dogs were allocated in the study with ticks infestation (n = 35) and fleas infestation (n = 15). on day 0, thirty-five tick and fifteen flea infested dogs received the test formulation, a combination of fipronil 9.8% (w/v) and (s)-methoprene 8.8% (w/v) spot-on solution. ticks and flea counts were taken o ...201627190688
evaluation and comparison of a flumethrin-imidacloprid collar and repeated monthly treatments of fipronil/(s)-methoprene to control flea, ctenocephalides f. felis, infestations on cats for eight months.this controlled laboratory study was designed to evaluate the efficacy of the 10 % imidacloprid/4.5 % flumethrin collar (seresto®, bayer animal health) against fleas (ctenocephalides f. felis) on cats, when compared to fipronil (9.8 %w/w)/(s)-methoprene (11.8 % w/w) topical spot-on formulation (frontline® plus for cats and kittens, merial).201627189592
detection of vector-borne pathogens in cats and their ectoparasites in southern italy.vector-borne pathogens are the subject of several investigations due to the zoonotic concern of some of them. however, limited data are available about the simultaneous presence of these pathogens in cats and their ectoparasites. the aim of the present study was to define the species of ectoparasites found on cats as well as to investigate vector-borne pathogens in cats and their ectoparasites in southern italy.201627160725
rickettsia felis: the complex journey of an emergent human pathogen.rickettsia felis is an obligate intracellular bacterium that is different from other officially recognized rickettsial species. it has multiple genes of different origins, an incubation temperature of less than 32°c, and a conjugative plasmid. this rickettsia is commonly detected in febrile patients in sub-saharan africa. r. felis is frequently detected in cat fleas, but recently mosquitoes have been suspected to be able to transmit the bacterium. however, many aspects of the ecology and epidemi ...201627155905
transmission mechanisms of an emerging insect-borne rickettsial pathogen.vector-borne pathogens must overcome arthropod infection and escape barriers (e.g. midgut and salivary glands) during the extrinsic incubation period (eip) before subsequent transmission to another host. this particular timespan is undetermined for the etiological agent of flea-borne spotted fever (rickettsia felis). artificial acquisition of r. felis by blood-feeding cat fleas revealed dissemination to the salivary glands after seven days; however, this length of time is inconsistent with co-fe ...201627117813
influence of bloodmeal host on blood feeding, egg production, and offspring sex ratio of ctenocephalides felis felis (siphonaptera: pulicidae).the cat flea, ctenocephalides felis felis (bouché), feeds on different host species, causing annoyance or transmitting disease agents. in this study, the influence of the host of the cat flea on blood feeding, egg production, and sex ratio of the offspring was investigated. two strains of c. felis were domesticated on either rats or mice for >10 yr in the laboratory, and in this study, these fleas were placed in the following groups and fed on rats or mice continuously: group a (rat-domesticated ...201627106931
infection by rickettsia felis in opossums (didelphis sp.) from yucatan, mexico.rickettsia felis is an emergent pathogen and the causative agent of a typhus-like rickettsiosis in the americas. its transmission cycle involves fleas as biological vectors (mainly ctenocephalides felis) and multiple domestic and synanthropic mammal hosts. nonetheless, the role of mammals in the cycle of r. felis is not well understood and many efforts are ongoing in different countries of america to clarify it. the present study describes for the first time in mexico the infection of two specie ...201627074326
molecular evidence of vector-borne pathogens in dogs and cats and their ectoparasites in algiers, algeria.in algeria, only limited information is currently available on the prevalence of emergent canine and feline vector-borne diseases. the aim of the present work was to detect by qpcr vector-associated bacteria in stray dogs and cats and their ectoparasites from algiers. 18/117 (15.38%) dogs and 2/107 (1.87%) cats were positive for at least one vector-borne agent. coxiella burnetii and bartonella henselae were identified in 1/117 (0.85%) dog individually. ehrlichia canis dna was detected in 17/117 ...201627012917
bartonella henselae in italy: a rare seasonal infection.symptomatic bartonella henselae infection is considered rare in europe. cat fleas transmit the microorganism between cats, but their role in transmission of b. henselae to humans has not been defined. the aim of our study was to perform a retrospective study of detected cases at our hospital.201627008366
open field study on the efficacy of oral fluralaner for long-term control of flea allergy dermatitis in client-owned dogs in ile-de-france region.fluralaner is the first orally administered isoxazoline to provide 12 weeks of activity against fleas and ticks after a single administration. as a result of its potent anti-flea activity, oral fluralaner may be proposed as a component of a strategy for the control of flea allergy dermatitis (fad) in dogs. the open field study reported here assessed the efficacy of fluralaner for long-term control (up to 6 months) of fad in affected client-owned dogs maintained under common household conditions ...201627007494
insecticide resistance in fleas.fleas are the major ectoparasite of cats, dogs, and rodents worldwide and potential vectors of animal diseases. in the past two decades the majority of new control treatments have been either topically applied or orally administered to the host. most reports concerning the development of insecticide resistance deal with the cat flea, ctenocephalides felis felis. historically, insecticide resistance has developed to many of the insecticides used to control fleas in the environment including carba ...201626999217
exposure of dogs to spotted fever group rickettsiae in urban sites associated with human rickettsioses in costa rica.the zoonotic transmission cycles of rickettsia rickettsii and other spotted fever group (sfg) rickettsiae in latin america have usually been associated with rural or sylvatic environments, although domestic dogs can be implicated in more populated settings. in this study, exposure of dogs to sfg rickettsiae in the greater metropolitan area of costa rica was investigated. dogs from sites associated with human cases and from dog shelters were evaluated by indirect immunofluorescence assay (ifa) us ...201626995323
discovery of sarolaner: a novel, orally administered, broad-spectrum, isoxazoline ectoparasiticide for dogs.the novel isoxazoline ectoparasiticide, sarolaner, was identified during a lead optimization program for an orally-active compound with efficacy against fleas and ticks on dogs. the aim of the discovery program was to identify a novel isoxazoline specifically for use in companion animals, beginning with de novo synthesis in the zoetis research laboratories. the sarolaner molecule has unique structural features important for its potency and pharmacokinetic (pk) properties, including spiroazetidin ...201626961590
evaluation of the speed of kill, effects on reproduction, and effectiveness in a simulated infested-home environment of sarolaner (simparica™) against fleas on dogs.four studies were conducted to evaluate the speed of kill, effect on egg production, and efficacy in a simulated infested-home environment of a novel isoxazoline, sarolaner (simparica™, zoetis), against fleas on dogs. individually identified and housed, purpose-bred beagles were used in each study and were allocated randomly to groups based on pretreatment parasite counts. in two speed of kill studies, groups of dogs infested with 100 fleas prior to treatment were treated orally with placebo or ...201626961589
synergism of the igrs methoprene and pyriproxyfen against larval cat fleas (siphonaptera: pulicidae).insect growth regulators (igrs) methoprene and pyriproxyfen are widely used as topical treatments to pets or applied to the indoor environment to control cat fleas, ctenocephalides felis (bouché). the toxicity of methoprene, pyriproxyfen, and combinations of both igrs to cat flea larvae was determined. the lc50 of methoprene and pyriproxyfen applied to larval rearing medium was 0.39 and 0.19 ppm, respectively. combinations of methoprene:pyriproxyfen in ratios of 1:1, 5:1, 10:1, and 20:1 produced ...201626957391
determination of the effective dose of a novel oral formulation of sarolaner (simparica™) for the treatment and month-long control of fleas and ticks on dogs.three laboratory studies were conducted to determine the appropriate dose of sarolaner, a novel isoxazoline, for the treatment and month-long control of infestations of fleas and ticks on dogs. in the first study, dogs were treated orally with sarolaner suspension formulations at 1.25, 2.5 or 5.0mg/kg, and infested with dermacentor reticulatus, rhipicephalus sanguineus ticks and with ctenocephalides felis felis (cat flea) prior to treatment and then weekly for up to 8 weeks. fleas and ticks were ...201626948830
efficacy and safety of sarolaner (simparica™) against fleas on dogs presented as veterinary patients in the united states.the efficacy and safety of a novel isoxazoline parasiticide, sarolaner (simparica™), for the control of fleas on dogs was evaluated in a randomized, controlled clinical study conducted in 19 general veterinary practices throughout the united states. four hundred and seventy nine (479) dogs from 293 households were enrolled. each household was randomly assigned to treatment with either sarolaner oral tablets (simparica™, zoetis) at the proposed label dose or an approved comparator product at the ...201626947964
evaluation of the effectiveness of a novel oral formulation of sarolaner (simparica™) for the treatment and control of fleas on dogs.the efficacy of a single oral dose of a novel isoxazoline, sarolaner (simparica™, zoetis), for the treatment and control of flea infestations on dogs was confirmed in five laboratory studies. the studies were conducted using adult purpose-bred beagles and/or mixed breed dogs. all animals were individually identified and housed, and were allocated randomly to treatment with either placebo or sarolaner (eight to 10 per group) based on pretreatment parasite counts. three studies used cat flea (cten ...201626935820
on some issues of human-animal studies: an introduction.animals are "in" - since prehistoric times when humans (or their ancient ancestors) were hunting animals, and when they fabricated the paleolithic dog as well as the paleolithic cat. in less general terms, animals are "in" since they received names and were listed, observed, mummified, turned into totems, and, later on, dissected, tortured under laboratory conditions, trained as experimental subjects or "purified" as model organisms. and they are massively "in" again, but now from overtly legal ...201626903370
molecular detection of rickettsia felis in humans, cats, and cat fleas in bangladesh, 2013-2014.high prevalence of rickettsia felis in patients with fever of unknown origin was revealed in the north-central bangladesh from 2012 to 2013. subsequently, in this study, prevalence of r. felis in cats and cat fleas (ctenocephalides felis), together with febrile patients, was studied by pcr detection of 17 kda antigen gene and dna sequencing. r. felis was detected in 28% (28/100) and 21% (14/68) of cat blood and cat flea samples, respectively, whereas 42% (21/50) of patients were positive for r. ...201626901499
bartonella species identified in rodent and feline hosts from island and mainland western australia.bacteria of the genus bartonella have been described in multiple mammalian hosts with many species capable of causing disease in humans. cats and various species of rats have been reported to play a role as vertebrate hosts to a number of bartonella spp. this study aimed to identify bartonella spp. in western australia, dirk hartog island (dhi), and christmas island (ci) and to investigate the presence of potential arthropod vectors. feral cats were collected from ci (n = 35), dhi (n = 23) and s ...201626900749
comparative speed of kill of sarolaner (simparica) and spinosad plus milbemycin oxime (trifexis) against induced infestations of ctenocephalides felis on dogs.fleas are a ubiquitous ectoparasite infesting dogs and cause direct discomfort, allergic reactions and are responsible for the transmission of several pathogens. the rapid speed of kill of a parasiticide is important to alleviate the direct deleterious effects of fleas, reduce the impact of allergic responses, and break the flea life cycle. in this study, the speed of kill of a novel orally administered isoxazoline parasiticide, sarolaner (simparica) against fleas on dogs was evaluated and compa ...201626896448
comparative speed of kill of sarolaner (simparica and fluralaner (bravecto) against induced infestations of ctenocephalides felis on dogs.fleas are the most common ectoparasite infesting dogs globally and cause direct discomfort, induce allergic reactions, and transmit pathogenic agents. rapid speed of kill is an important characteristic for a parasiticide in order to alleviate the direct deleterious effects of fleas, reduce the impact of allergic responses, and break the flea life cycle. in this study, the speed of kill of a novel, orally administered isoxazoline parasiticide, sarolaner (simparica), against fleas on dogs was eval ...201626896436
comparative speed of kill of sarolaner (simparica) and afoxolaner (nexgard) against induced infestations of ctenocephalides felis on dogs.fleas are the most common ectoparasite infesting dogs globally. the many possible sequellae of infestation include: direct discomfort; allergic reactions; and the transmission of pathogens. rapid speed of kill is an important characteristic for a parasiticide in order to alleviate the direct deleterious effects of fleas, reduce the impact of allergic responses, and break the flea infestation cycle. in this study, the speed of kill of a novel orally administered isoxazoline parasiticide, sarolane ...201626896428
in vitro efficacy of essential oils and extracts of schinus molle l. against ctenocephalides felis felis.extracts and essential oils from plants are important natural sources of pesticides. these compounds are considered an alternative to control ectoparasites of veterinary importance. schinus molle, an endemic species of brazil, produces a high level of essential oil and several other compounds. the aim of this work was to determinate the chemical composition of extracts and essential oils of s. molle and further to evaluate the activity against eggs and adults of ctenocephalides felis felis, a pr ...201626887529
knock-down and speed of kill of a combination of fipronil and permethrin for the prevention of ctenocephalides felis flea infestation in dogs.a topical combination of fipronil + permethrin (frontline tri-act/frontect, merial) has recently been developed to control fleas, ticks, mosquitoes, sandflies and stable flies on dogs. two studies were conducted to assess its speed of kill and knock-down effect on ctenocephalides felis fleas. the combination was compared to either fipronil alone or to a combination of permethrin, dinotefuran, and pyriproxyfen,201626830931
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