chromosomal control of foot pad development in sarcophaga bullata. 3. requirement of rna and protein synthesis for cuticle formation and tanning. | | 1972 | 4670875 |
effects of ecdysone analogues on development and metabolic activity of wing disks of the fleshfly, sarcophaga peregrina, in vitro. | | 1973 | 4708145 |
the photoperiodic clock in the flesh-fly, sarcophaga argyrostoma. | | 1973 | 4752418 |
effect of the exotoxin of bacillus thuringiensis on normal and ecdysone-stimulated ribonucleic acid polymerase activity in intact nuclei from the fat-body of sarcophaga bullata larvae. | a nuclear preparation from the fat-body of sarcophaga bullata was obtained which incorporates nucleotides at a steady rate. two activities, differing in their response to alpha-amanitin, are present. the activities are not separated by changes in the concentration of (nh(4))(2)so(4) as effectively as in mammalian nuclei. the activity resistant to alpha-amanitin is stimulated by ecdysone, and both normal and the ecdysone-stimulated activities are inhibited by the exotoxin. the amanitin-sensitive ... | 1973 | 4797896 |
effect of an exotoxin from bacillus thuringiensis on deoxyribonucleic acid-dependent ribonucleic acid polymerase in nuclei from adult sarcophaga bullata. unusual behaviour of eukaryotic polymerases to inhibitors. | the dna-dependent rna polymerase activities in nuclei isolated from adult sarcophaga bullata are unusual in their responses to metal ions, ionic strength and inhibitors. there is an activity that is sensitive both to rifamycin and to alpha-amanitin. the activity is less sensitive to bacillus thuringiensis exotoxin than is larval polymerase, and low concentration of exotoxin provoke a slight stimulation. | 1973 | 4797897 |
the appearance of dopa decarboxylase activity in imaginal discs of sarcophaga bullata, undergoing development in vitro. | | 1974 | 4831109 |
two cases of human sarcophaga (diptera: sarcophagidae) myiasis in quebec, with descriptions of the larvae. | | 1974 | 4840657 |
effect of x-irradiation on the formation of the puparium in the fleshfly, sarcophaga bullata. | | 1974 | 4851715 |
new observations on flagellar fine structure. the relationship between matrix structure and the microtubule component of the axoneme. | the sperm flagella of the blowfly sarcophaga bullata demonstrate the relationship of radial projections in the matrix region to the microtubule organization of the axoneme. the a microtubule of each peripheral doublet is connected to the central sheath by a series of paired radial links. the links lie along the tubule wall with a alternate spacing of about 320/560 a. the distal end of each link is enlarged into a globular head that connects via a transitional link to the helical sheath around th ... | 1970 | 4935335 |
squalene metabolism in the larva of the fly, sarcophaga bullata. | | 1972 | 5009197 |
rates and cycles of oxygen consumption during pupal diapause in sarcophaga flesh flies. | | 1972 | 5024062 |
sarcophaga macroauriculata ho as a facultative parasite in darjeeling. | | 1972 | 5071096 |
the cytological effect of ecdysterone on the midgut cells of the flesh-fly sarcophaga bullata. | larvae of the flesh-fly, sarcophaga bullata, were injected with the synthetic moulting hormone ecdysterone or saline at the beginning of the third and final larval instar. one group was left untreated. the ecdysterone-injected larvae showed an increase in number of secondary lysosomes in the midgut epithelial cells similar to that observed at the onset of metamorphosis, an event which would normally occur about 48 hr later in these larvae. | 1971 | 5092208 |
mevalonate kinase: localization and variation in activity during the development of sarcophaga bullata. | | 1971 | 5094244 |
embryonic determination of pupal diapause in the flesh fly sarcophaga crassipalpis. | | 1971 | 5097106 |
dietary sterol effects on mevalonate kinase activity in axenic and non-axenic sarcophaga bullata (diptera). | | 1971 | 5111035 |
sugar receptor specificity in the fleshfly, sarcophaga bullata. | | 1971 | 5135541 |
ii. culture of cells of the flesh fly, sarcophaga bullata. | | 1971 | 5142321 |
[radiation induced shortening of life of sarcophaga peregrina and its modification by dose fractionation]. | | 1965 | 5217120 |
[radiation effects on the respiration and dna synthesis in the pupa of sarcophaga peregrina]. | | 1967 | 5232505 |
[the relation of "recovery" and mitosis in irradiated sarcophaga pupae]. | | 1967 | 5232507 |
[effects of x-rays on respiration of sarcophaga pupae]. | | 1966 | 5232743 |
[age-dependent radiosensitivity in adult sarcophaga peregrina]. | | 1968 | 5248445 |
[independence of the inhibition of imago formation in sarcophaga pupae upon the dose-rate relationship]. | | 1968 | 5251460 |
[recovery and accumulation of radiation effects at low dose-rate in sarcophaga pupae]. | | 1970 | 5266372 |
[effects of anoxic conditions on radiation-induced life-shortening in sarcophaga adults]. | | 1970 | 5276366 |
the role of beta-alanyl-l-tyrosine (sarcophagine) in puparium formation in the fleshfly sarcophaga bullata. | | 1969 | 5347606 |
perception of spatial relationships and pupation delay in fly larvae (sarcophaga barbata). | | 1969 | 5349581 |
chromosomal control of foot pad development in sarcophaga bullata. i. the puffing pattern. | | 1969 | 5367550 |
an intracellular study of the myocardial cells of the flesh fly, sarcophaga bullata. | | 1970 | 5417455 |
neuronal properties of the neurosecretory cells in the fly sarcophaga bullata. | | 1970 | 5417495 |
trehalases of the fleshfly sarcophaga barbata. | | 1970 | 5432383 |
inactivation of alpha-ecdysone and cyasterone by larvae of the fleshfly, sarcophaga peregrina, and pupae of the silkworm, samia cynthia. | | 1970 | 5433302 |
acceleration of puparium formation in sarcophaga argyrostoma by electrical stimulation or scorpion venom. | | 1970 | 5433720 |
chromosomal control of foot pad development in sarcophaga bullata. ii. cuticle formation and tanning. | | 1970 | 5460490 |
electrical correlates of neural superposition in the eye of the fly sarcophaga bullata. | | 1970 | 5470513 |
specificity and locale of the l-3-glycerophosphate-flavoprotein oxidoreductase of mitochondria isolated from the flight muscle of sarcophaga barbata thoms. | 1. the oxidation of l-3-glycerophosphate by flight-muscle mitochondria isolated from the flesh fly sarcophaga barbata has been studied. use of substrate analogues indicates that the catalytic and effector l-3-glycerophosphate binding sites on the allosteric l-3-glycerophosphate-flavoprotein oxidoreductase differ markedly in specificity. 2. the l-3-glycerophosphate-cyanoferrate oxidoreductase system in these mitochondria is antimycin-insensitive whereas the corresponding nadh-cyanoferrate oxidore ... | 1970 | 5499959 |
[vaginal myiasis due to sarcophaga argyrostoma (rob.-desvoidy)]. | | 1970 | 5512982 |
juvenile hormone: effects on a higher dipteran. | injection of dl-juvenile hormone or c(17) methyl ester into sarcophaga bullata larvae prevents puparium formation or arrests development at about the 3rd day of pupal-adult development. topical application to the abdomens of young pupae results in the secretion of a second pupal cuticle. this is the first reported morphogenetic effect of juvenile hormone on a fly. | 1968 | 5659127 |
fine structural features of developing leg discs of the blowfly, sarcophaga bullata (parker). | | 1968 | 5704103 |
the endocrine and nutritional control of egg maturation in the fleshfly sarcophaga bullata. | | 1968 | 5761654 |
the fine structure of neurons and nerve fibres in the thoracic ganglion of the blowfly, sarcophaga bullata. | | 1968 | 5761662 |
hormonal control of turnover of tyrosine and tyrosine phosphate during tanning of the adult cuticle in the fly, sarcophaga bullata. | | 1969 | 5768888 |
bursicon mediation of tyrosine hydroxylation during tanning of the adult cuticle of the fly, sarcophaga bullata. | | 1969 | 5772204 |
synthesis of digestive enzyme in the fleshfly sarcophaga bullata stimulated by food. | | 1969 | 5786202 |
beta-alanyl-l-tyrosine. chemical synthesis, properties and occurrence in larvae of the fleshfly sarcophaga bullata parker. | 1. a naturally occurring peptide, termed sarcophagine, has been isolated from larvae of the fleshfly sarcophaga bullata. 2. the physicochemical properties of the natural peptide are identical with those of synthetic beta-alanyl-l-tyrosine. 3. during larval growth the concentration of the dipeptide increases continuously; in the fully grown larva it is the predominant non-protein ninhydrin-positive material. 4. sarcophagine is rapidly and extensively utilized at the time of hardening and darkenin ... | 1969 | 5821010 |
x-ray induced shortening of life span of adult sarcophaga peregrina and its modification by dose fractionation. | | 1965 | 5832640 |
ontogenetic fate of the neurosecretory cells in the larval brain of sarcophaga ruficornis (fabricius, 1794) (diptera: cyclorrhapha). | | 1965 | 5844187 |
phospholipid composition of fat-bodies of sarcophaga bullata. | | 1965 | 5848791 |
differential deoxyribonucleic acid replication in the giant foot-pad cells of sarcophaga bullata. | | 1965 | 5868851 |
the effect of diet on the hemolymph amino acid constituents of the larvae and pupae of sarcophaga ruficornis (fabricius): (diptera). | | 1965 | 5870512 |
properties of bursicon: an insect protein hormone that controls cuticular tanning. | on gel filtration the retention volume of bursicon indicates a molecular weight of about 40,000. on disc electrophoresis bursicon migrates toward the anode and appears at an r(f) (relative to the marker dye) of 0.3 to 0.4. the properties of fractions with bursicon activity in blood, brain, and ganglion of a fly (sarcophaga bullata), and in blood, ganglion, and corpora cardiaca of a roach (periplaneta americana) are similar, but not identical. bursicon in the blood is more heat labile, and the ac ... | 1966 | 5908970 |
curare as a neuromuscular blocking agent in insects. | topical application of curare produced no effects on electrical activity in single flight-muscle fibers of the fly sarcophaga. however, the intraabdominal injection of curare induced a neuromuscular block similar to that described for vertebrates. the general refractoriness of some insects to chemical agents may well be due to the method of application. | 1966 | 5919751 |
effects of x-irradiation on the development of the respiratory system and survival time in pupae of sarcophaga peregrina. | | 1966 | 5971336 |
the larval fat body of sarcophaga bullata (diptera) as a system for studying phospholipid biosynthesis. | | 1966 | 6004822 |
[a case of mixed otomyiasis in ivory coast]. | authors relate the first case of otomyiasis in ivory coast due to chrysomyia bezziana and sarcophaga sp. they insist on scarcity of such a localisation. egg-laying and development of larvae in tympanum case was favoured by a preexisting cholesteatoma. precise entomological diagnosis needs incubation of the larvae in petri dishes. | 1984 | 6091936 |
accumulation of cadmium and induction of its binding protein in the digestive tract of fleshfly (sarcophaga peregrina) larvae. | the larva of sarcophaga peregrina ( fleshfly ) was fed with cadmium (cd)-containing diet and the distribution of cd among tissues was determined by separating each organ. approximately 90% of cd accumulated in the larva was found in the digestive tract, the fat body and the malpighian tube being less effective tissues in its accumulation. cd in the digestive tract was mostly bound to an inducible cd-binding protein. the cd-binding protein was a mixture of five isoproteins having several properti ... | 1984 | 6144428 |
excretion of cadmium and change in the relative ratio of iso-cadmium-binding proteins during metamorphosis of fleshfly (sarcophaga peregrina). | cadmium (cd) was accumulated in the larvae of sarcophaga peregrina (fleshfly) by exposure through diet, and the metabolic fate of the accumulated cd during metamorphosis was examined. most of the cd accumulated in the larvae was retained in the pupae, and 53% of that was excreted from the adults immediately after emergence. the cd-binding protein induced in the larvae was degraded after pupation and the relative ratio of iso-cd-binding proteins re-induced in the adults was different from that in ... | 1984 | 6149071 |
identification of storage-protein messenger rna of the fleshfly sarcophaga peregrina. | storage-protein mrna was found to be abundant in poly(a)-containing rna extracted from the fat-body of third-instar larvae of sarcophaga peregrina (fleshfly). this rna sedimented at the position of 19s on sucrose-density-gradient centrifugation and the product of its translation in vitro was 75k protein (protein of mol.wt. 75 000), which was precipitated specifically with antibody against storage protein. this product was suggested to contain a signal sequence that is missing in mature storage p ... | 1982 | 6180730 |
sequence arrangement of the rrna genes of the dipteran sarcophaga bullata. | velocity sedimentation studies of rna of sarcophaga bullata show that the major rrna species have sedimentation values of 26s and 18s. analysis of the rrna under denaturing conditions indicates that there is a hidden break centrally located in the 26s rrna species. saturation hybridization studies using total genomic dna and rrna show that 0.08% of the nuclear dna is occupied by rrna coding sequences and that the average repetition frequency of these coding sequences is approximately 144. the ar ... | 1981 | 6269054 |
identification and activation of storage protein receptor of sarcophaga peregrina fat body by 20-hydroxyecdysone. | previous work showed that 20-hydroxyecdysone activates the fat body of sarcophaga peregrina larvae to incorporate storage protein selectively from the hemolymph. in this study, storage protein receptors of the fat body membrane which were induced on pupation or on treatment of larval fat body with 20-hydroxyecdysone in vitro were identified. the binding of storage protein to its receptor required divalent cations, especially ca2+, and the binding was very sensitive to ph. the storage protein rec ... | 1983 | 6313643 |
mode of action of a bactericidal protein induced in the haemolymph of sarcophaga peregrina (flesh-fly) larvae. | the mode of action of a bactericidal protein (sarcotoxin i) purified from the haemolymph of sarcophaga peregrina (flesh-fly) larvae was studied, focusing attention on its effect on the function of the membrane of escherichia coli. sarcotoxin i almost completely blocked the uptakes of tetraphenylphosphonium ion and proline, which are known to be driven by a membrane potential, indicating that this protein disrupts the membrane potential of bacteria. | 1984 | 6383355 |
induction of human gamma interferon by sarcophaga peregrina humoral lectin. | humoral lectin isolated from the hemolymph of injured sarcophaga peregrina (flesh-fly) larvae was found to activate human peripheral blood cells to produce interferon activity. this interferon was inactivated by dialysis against a solution of ph 2.0 and by heat treatment at 56 degrees c for 30 min, indicating that it was a gamma interferon. the role of this lectin in the defence mechanism is discussed from the viewpoint of comparative immunology. | 1984 | 6434349 |
induction of tnf-like factor by murine macrophage-like cell line j774.1 on treatment with sarcophaga lectin. | on stimulation with sarcophaga lectin, the mouse macrophage-like cell line j774.1 secreted a factor like the tumor necrosis factor (tnf) into the culture medium. this factor was a protein with a molecular weight of 40000-45000, and was cytotoxic to l-929 cells, but not to normal embryonic fibroblasts. this factor was effective on both the ascites form and solid form of sarcoma 180 transplanted into icr mice. | 1984 | 6479338 |
identification of storage protein receptor and its precursor in the fat body membrane of sarcophaga peregrina. | a previous paper (ueno, k., ohsawa, f., and natori, s. (1983) j. biol. chem. 258, 12210-12214) showed that a storage protein receptor is present in membranes of the pupal fat body of sarcophaga peregrina, whereas membranes of the larval fat body contain a cryptic receptor, and that 20-hydroxyecdysone is essential for activation of the cryptic receptor to bind and incorporate the storage protein. the present paper reports that the storage protein receptor is a membrane protein of the pupal fat bo ... | 1984 | 6480601 |
activation of murine macrophage-like cell line j774.1 by sarcophaga peregrina lectin. | the effect of sarcophaga lectin on mouse macrophage-like cell line j774.1 was studied. it was found that this lectin induced significant morphological changes and stimulated glucose consumption of j774.1 cells. a similar effect was observed when bacterial lipopolysaccharide was added to the culture medium. however, although cells treated with lipopolysaccharide were not cytotoxic to allogeneic tumor cells, those treated with sarcophaga lectin were found to acquire cytotoxicity. the physiological ... | 1984 | 6489679 |
metamorphic changes in the hindgut of sarcophaga ruficornis with special reference to the development of rectal pads. | in sarcophaga ruficornis, during normal metamorphosis the larval hindgut epithelium degenerates and the imaginal hindgut epithelium is formed from the proliferation and differentiation of the cells of the posterior imaginal ring. the newly formed hindgut becomes slightly dilated posteriorly to form the rectal pouch. at four places in this pouch, the epithelium becomes thick and forms the primordia of the rectal pads. the cells in the primordia begin to divide and project into the lumen of the po ... | 1984 | 6516915 |
differential expression of two abundant messenger rnas during development of sarcophaga peregrina. | expression of two abundant mrnas in the fat body of third instar larvae of sarcophaga peregrina was studied using genomic clones of storage protein and 25k protein as probes. it was found that these two genes were expressed sequentially in the third instar with a 20-hr interval between their expressions. no amplification of genes was detected during larval development, suggesting that the high level of mrna was due to efficient transcription. the contents of the two mrnas in third instar larvae ... | 1983 | 6617997 |
on neuronal homology: a comparison of similar axons in musca, sarcophaga, and drosophila (diptera: schizophora). | thoracic axons occurring in musca and sarcophaga are similar to those previously reported in the giant fiber pathway of drosophila. serial section reconstruction of both species has shown that the cervical giant fiber descending from the brain into the thoracic ganglion and the thoracic motor axon innervating the tergotrochanteral muscle follow courses matching those of similar axons in drosophila. likewise in both musca and sarcophaga a thoracic axon establishes axoaxonal synapses onto dorsal l ... | 1983 | 6619326 |
polymorphonuclear leukocyte-mediated cytolysis induced by animal lectin. | nine animal lectins, i.e., sarcophaga peregrina agglutinin, balanus roseus agglutinin, aplysia kurodai agglutinin, balanus balanoides agglutinin, tetraclita squamosa japonica agglutinin, misgurnus anguillicaudatus lectin, asterina pectinifera agglutinin, helix aspersa agglutinin and helix pomatia agglutinin, were tested for induction of cytolysis mediated by polymorphonuclear leukocytes. among them, s. peregrina agglutinin and b. roseus agglutinin lysed murine target cells in co-operation with p ... | 1983 | 6628907 |
influence of age and diet on dna synthesis and the dna content of mid-gut cells in the dipteran sarcophaga carnaria. | virgin female sarcophaga carnaria maintained on a diet of sugar and water had shorter lifespans than those maintained on a liver, sugar and water diet; there was no significant different between the lifespans of mated females or mated males kept on the two diets. autoradiographs of mid-gut sections from virgin females which had been fed with [3h]thymidine showed substantial amounts of dna synthesis in the nuclei during at least the first 14-15 days of adult life. cytophotometric measurements of ... | 1983 | 6656299 |
sterol-free eukaryotic cells from continuous cell lines of insects. | three cell lines from mamestra brassicae (lepidoptera), one from aedes albopictus (diptera) and one from sarcophaga peregrina (diptera) were continuously cultured in a sterol-free medium and analyzed for sterol content. no sterols were found in any of the cell lines, whereas from cells cultured in a serum-containing medium, cholesterol was detected. this is the first instance of sterol-free animal cells. | 1983 | 6667502 |
identification of testosterone and progesterone in hemolymph of larvae of the fleshfly sarcophaga bullata. | testosterone- and progesterone-like substances were detected by radioimmunoassay (ria) in chromatographed extracts of hemolymph from larvae of sarcophaga bullata (s.b.). gas chromatographic (gc) analysis after heptafluorobutyric acid (hfba) derivation of hemolymph extracts, purified by paper and silica gel column chromatography, showed a peak in the gc recordings with the same retention time as the hfba derivative of pure testosterone. a testosterone concentration of 92 ng/100 ml hemolymph was f ... | 1983 | 6667840 |
metabolism of injected [3h]ecdysone in male sarcophaga bullata (diptera). | within 30 min after injection, [3h]ecdysone was rapidly partially metabolized to ecdysterone and other ecdysteroids. after 8 hr most (99%) of the tritiated material had disappeared from the hemolymph. in testes, the predominant ecdysteroid appeared to be ecdysterone but no accumulation occurred. comparison of the dynamics of the ecdysone metabolism in abdomens and head-thorax sections showed that in the head-thorax ecdysterone was the major component, whereas in abdomens the major metabolites we ... | 1983 | 6667841 |
multi-gene structure of the storage protein genes of sarcophaga peregrina. | three recombinant phages containing chromosomal segments of sarcophaga peregrina encoding storage protein were isolated. these clones were found to contain sequences hybridized with the messenger rna of 75,000 mr protein, a monomer of storage protein. from restriction analysis it was found that message-homologous regions are not identical, and thus are different genes with similar sequences. from the analysis of these clones it was concluded that the storage protein gene belongs to a multi-gene ... | 1984 | 6716476 |
identification of hemagglutinating protein and bactericidal activity in the hemolymph of adult sarcophaga peregrina on injury of the body wall. | when the body wall of adult sarcophaga peregrina (flesh-fly) was injured with a hypodermic needle, hemagglutinating activity and antibacterial activity were induced in the hemolymph simultaneously. the hemagglutinating activity was shown to be due to the same lectin that was found previously in the hemolymph of injured larvae. thus, larval and adult sarcophaga respond in the same way to injury of the body wall. | 1984 | 6734869 |
in vitro activity of dipteran ring glands and activation by the prothoracicotropic hormone. | the relationship between hemolymph ecdysteroid titer, ring gland (rg) activity, and prothoracicotropic hormone (ptth) activation of rg in vitro has been examined during the postfeeding larval, prepupal, and pupal stages of sarcophaga bullata. using the ecdysteroid radioimmunoassay (ria), two significant peaks were recorded during the red spiracular stage and during the first few hours after the formation of the white prepupa and a third large peak 9 hr later. it is postulated that these increase ... | 1984 | 6735164 |
sarcophagine (beta-alanyl-l-tyrosine) synthesis in the fat body of sarcophaga peregrina larvae. | the fat body of sarcophaga peregrina larvae was found to have activity for synthesis of sarcophagine (beta-alanyl-l-tyrosine). this activity was due to a soluble enzyme (sarcophagine synthetase) that requires mg2+ and atp as cofactors. the enzyme activity decreased significantly after puparium formation and no sarcophagine synthesis was detected when fat body from white pupae was incubated in vitro with 3h-beta-alanine. this apparent loss of sarcophagine synthesis was found to be partly due to p ... | 1984 | 6746587 |
purification of lectin induced in the hemolymph of sarcophaga peregrina larvae on injury. | a lectin was purified from the hemolymph of sarcophaga peregrina larvae, obtained after injury of their body wall. this lectin agglutinated sheep red blood cells markedly and the hemagglutinating activity was inhibited by galactose and lactose. the active lectin was found to have a molecular weight of 190,000 and to consist of four alpha subunits and two beta subunits, with molecular weights of 32,000 and 30,000, respectively. during the early pupal stage, similar hemagglutinating activity in th ... | 1980 | 6766942 |
a possible mechanism of induction of insect lectin. | studies were made using antibody against a lectin induced in the hemolymph of sarcophaga peregrina larvae on injury. the results suggested that the alpha subunit (mr = 32,000) of the lectin is normally present in the hemolymph. on injury of the body wall, part of the alpha subunit seems to be converted to the beta subunit (mr = 30,000), probably by partial proteolysis with a protease activated in response to the injury. as a result, an active lectin with the structure alpha 4 beta 2 is formed. | 1981 | 6788759 |
measurement of sarcophaga peregrina lectin under various physiological conditions by radioimmunoassay. | lectin induced in the hemolymph of sarcophaga peregrina (flesh fly) larvae on injury of the body wall or on pupation was studied further by radioimmunoassay, focusing on the interaction between the lectin and hemocytes. it was found that the amount of lectin on the surface of hemocytes prepared from injured larvae increased with time after injury of the body wall. radioiodinated lectin could bind to hemocytes prepared from injured larvae more effectively than those from normal larvae, indicating ... | 1983 | 6822551 |
change in free amino acids and phospholipids in the head of adult sarcophaga peregrina with age. | the amino acid and phospholipid contents in the head of adult sarcophaga peregrina (flesh-fly) were measured with age. only aspartic acid was found to increase significantly with aging. a significant difference was also detected in the fatty acid composition of the phospholipids. phospholipids isolated from old flies contained more unsaturated fatty acid than young flies, suggesting that the properties of the membrane of head cells change with age. these changes were observed irrespective of sex ... | 1983 | 6873527 |
purification and characterization of an antibacterial protein from haemolymph of sarcophaga peregrina (flesh-fly) larvae. | three antibacterial proteins were induced when the body wall of sarcophaga peregrina (flesh-fly) larvae was injured with a hypodermic needle. these proteins were separated and one was purified to homogeneity. the molecular weight of the purified protein was 5000 and its amino acid composition was similar to that of cecropins, which are antibacterial proteins in hyalophora cecropia (cecropia moth) pupae. this protein was found to have bactericidal activity and to be effective at a concentration o ... | 1983 | 6882366 |
gingival myiasis caused by diptera (sarcophaga). | a unique case of true and accidental gingival myiasis caused by diptera, sarcophaga (flesh fly, housefly) is reported. clinical findings are presented and a possible etiology is discussed. | 1980 | 6928286 |
relative growth of oocyte, nurse cells and follicular epithelium during oogenesis in the fly sarcophaga ruficornis (diptera). | the relative growth of the vitellarial components of sarcophaga ruficornis, a flesh fly, is recorded both in linear dimensions and volumes for each of the 12 stages of their development. the oocyte grows enormously, up to 24,828 times initial volume, the oocyte nucleus during the same period increases 159 times initial volume. the nurse cells and follicular epithelial cells grow only during the previtellogenic stages and decline thereafter in the process of furnishing nourishment to the oocyte, ... | 1982 | 7129202 |
induction of macrophage-mediated tumor lysis by an animal lectin, sarcophaga peregrina agglutinin. | several animal lectins, such as sarcophaga peregrina agglutinin, limulus polyhemus agglutinin, helix pomatia agglutinin and helix aspersa agglutinin, were tested for induction of tumor lysis mediated by macrophages. among them, only s. peregrina agglutinin purified from the hemolymph of s. peregrina larvae lysed tumor cells in co-operation with macrophages from the peritoneal cavity of mice. s. peregrina agglutinin alone did not kill target tumor cells. macrophages in the presence of this lectin ... | 1982 | 7152198 |
inactivation and reactivation of 20-hydroxyecdysone during pupal-adult development of the fleshfly, sarcophaga peregrina. | the inactivation process of ecdysteroids during pupal-adult development of the fleshfly, sarcophaga peregrina, was analyzed by following injected 3h-20-hydroxyecdysone, 20-hydoxyecdysone was rapidly metabolized and converted mostly into three groups of metabolites, one of which was closely related to 20-hydroxyecdysone in its chemical nature and the other two were polar conjugates of 20-hydroxyecdysone (oa and ob). the first group of metabolites decreased through the first day after injection of ... | 1980 | 7218574 |
human cases of furuncular, traumatic, and nasal myiasis in connecticut. | case histories are reported for four persons who had furuncular, traumatic, and nasal myiasis in connecticut during 1980. third-stage sarcophaga sp. and musca domestica larvae were removed from sores or wounds, a 2nd-stage cuterebra sp. larva was dislodged from cutaneous tissue, and 3rd-stage larvae of phaenicia sericata were extracted from nasal membranes. human exposure to these facultative or obligate dipteran parasites occurred at residences in urban and suburban settings, including a conval ... | 1981 | 7258501 |
gluconeogenesis in the dipteran fleshfly, sarcophaga ruficornis. | | 1981 | 7275148 |
moulting hormone, juvenile hormone and the ultrastructure of the fat body of adult sarcophaga bullata (diptera). | in the ovoviviparous fly, sarcophaga bullata, vitellogenesis in cyclic; a process reflected in ultrastructural changes in the fat body cells and oenocytes. at eclosion the larval fat body has not yet completely disappeared. during vitellogenesis the fat body cells are specialized for intensive protein synthesis showing a very extensive rer and numerous invaginations of the plasma membrane. these features disappear when the eggs descend into the oviducts to complete embryogenesis. the predominant ... | 1981 | 7307050 |
activation of latent ribonuclease in the fat-body of fleshfly (sarcophaga peregrina) larvae on pupation. | a crude extract of the fat-bodies of third-instar larvae of sarcophaga peregrina (fleshfly) was found to contain latent rnaase (ribonuclease) consisting of rnaase and inhibitor protein that is sensitive to p-chloromercuribenzoic acid. the rnaase activity in the crude extract of fat-bodies became detectable with time after puparium formation, indicating that the inhibitor is selectively inactivated and rnaase is released from the rnaase-inhibitor complex during metamorphosis. | 1981 | 7317010 |
stereochemistry of the hydrolysis of trehalose by the enzyme trehalase prepared from the flesh fly sarcophaga barbata. | 1. the enzyme trehalase was prepared from whole flesh flies, sarcophaga barbata. 2. the rate of mutorotation of glucose released by the enzyme was compared with that of alpha-d-glucose, beta-d-glucose and an equimolar mixture of the two. 3. derivatives of the glucose released by the enzyme were prepared and analysed by gas chromatography for anomeric composition. 4. the enzyme was incubated with trehalose in a medium enriched with h218o and the glucose was analysed by gas chromatography/mass spe ... | 1980 | 7341233 |
penetrability of orally toxic protein from cobra venom through the gut of a blowfly. | the oral toxicity of a radioiodinated toxic polypeptide isolated from a cobra snake venom as assayed by sarcophaga falculata blowflies coupled with assays on competitive displacement have indicated that: (a) during 3--4 h 8% of the orally active toxin is able to pass through the digestive system of the fly; (b) the orally active toxin after passing the gut binds to body tissues. the strong affinity of the toxin to tissue membranes explains its absence in the insect's hemolymph following oral app ... | 1980 | 7353052 |
penetrability of proteins through the digestive system of sarcophaga falculata blowfly. | on the background of the previous findings concerning the passage of a low molecular basic protein from cobra snake venom through the gut of a blowfly (primor, n., teitelbaum, z. and zlotkin, e. (1980) biochim, biophys, acta 627, 71--81) several additional proteins were tested in the present study. with the aid of toxicity tests, radioiodinated proteins, gel filtration chromatography, and the usage of sarcophaga falculata flies as test animals, the following information was obtained: 1. out of t ... | 1980 | 7353053 |
changes in head proteins of sarcophaga peregrina with age. | | 1980 | 7358184 |
autonomous puffing patterns in thoracic and abdominal polytene bristle cell chromosomes of the flesh fly sarcophaga barbata. | | 1980 | 7372010 |
on the growth of the proximal oocyte of the fly, sarcophaga ruficornis (diptera): 1. nutritional control. | sarcophaga ruficornis can survive in the laboratory if given sugar and water but is not able to show any appreciable growth of the proximal oocyte. when meat is given in addition to sugar and water, either from the time of emergence or from the fourth day of emergence, tremendous increase in the length and volume of this oocyte is observed. the growth of the oocyte is comparatively greater in the mated flies than in the virgin ones. the possible influence of pheromones or other sex signals was e ... | 1980 | 7380326 |
structure and metamorphic changes in the brain of the flesh fly sarcophaga ruficornis fabr. (diptera: sarcophagidae). | the larval brain is invested in a thin non-nucleated neurilemma below which lies a cellular perineurium. the brain lacks the central complex but feebly developed pedunculate bodies are present. the visual centres as represented by three optic lobe anlagen placed laterally to the protocerebrum. the deutocerebrum and tritocerebrum are represented by simple, small and rounded medullary masses. in adult, the central complex comprises of well developed central body, ellipsoid body, ventral tubercles ... | 1980 | 7400580 |
dermal myiasis in man caused by sarcophaga krameri (boettcher) (diptera: sarcophagidae) in malaysia. | | 1980 | 7403944 |
peripheral nervous system of the larva and adult of sarcophaga ruficornis fabr. (diptera: sarcophagidae). | in larva the thoracic and abdominal ganglia of ventral nerve cord are amalgamated into a thoracoabdominal ganglionic "mass". 3 thoracic and 8 paired lateral segmental nerves arise from the "mass" and innervate 1st to 8th abdominal segments. besides these, 7 mid dorsal nerves arise and bifurcate into left and right branches. these innervate the musculature of the 2nd to 8th abdominal segments. the suboesophageal ganglion is incorporated in the brain. in adult, the cervical connective separates th ... | 1980 | 7451947 |