| [study of the co 2 sensitivity symptom induced by vesicular stomatitis virus in drosophila melanogaster. i. vsv of the new jersey serotype and the cocal virus]. | | 1971 | 4329708 |
| [study of the symptom of co 2 sensitivity induced by the vesicular stomatitis virus in drosophila melanogaster. ii. vsv indiana serotype]. | | 1972 | 4340550 |
| natural selection of mutants of vesicular stomatitis virus by cultured cells of drosophila melanogaster. | | 1973 | 4355329 |
| properties of alcohol dehydrogenase isozymes in a strain of drosophila melanogaster homozygous for the adh-slow allele. | | 1974 | 4363212 |
| [vesicular stomatitis virus induced co2 sensitivity in "drosophila melanogaster". 3. a study of different serotypes of the virus (author's transl)]. | | 1973 | 4371090 |
| microspectrophotometry of arthropod visual screening pigments. | absorption spectra of visual screening pigments obtained in vitro with a microspectrophotometer using frozen sections are given for the insects musca domestica, phormia regina, libellula luctuosa, apis mellifera (worker honeybee only), drosophila melanogaster (wild type only) and the arachnids lycosa baltimoriana and lycosa miami. the spectral range covered is 260-700 nm for lycosa and drosophila and 310-700 nm for the remainder of the arthropods. a complete description of the instrumentation is ... | 1972 | 4623852 |
| action of insect hormones at the cellular level. morphological changes of a diploid cell line of drosophila melanogaster, treated with ecdysone and several analogues in vitro. | | 1972 | 4628122 |
| the effect of oxygen on the frequency of somatic recombination in drosophila melanogaster. | the influence of oxygen on the frequency of somatic recombination in the yellow singed system on the x chromosome of drosophila melanogaster was studied under a variety of experimental conditions. flies raised from egg to adult in atmospheres containing 70-90% oxygen were found to have significantly more mosaic spots on their abdominal tergites than were observed in flies which developed in air. first instar larvae x-rayed in from 0 to 100% oxygen demonstrated the existence of an oxygen effect f ... | 1972 | 4630584 |
| lethality patterns and morphology of selected lethal and semi-lethal mutations in the zeste-white region of drosophila melanogaster. | aspects of the developmental genetics of lethal and semi-lethal mutants representing 13 complementation groups (cistrons) in the 3a-3c region of the x chromosome of drosophila melanogaster are given. each of these cistrons is associated with a particular chromomere in the salivary gland chromosome. mutants within each cistron have similar lethality patterns and morphological attributes, and the characteristics of a given cistron are distinct with respect to other cistrons. these results provide ... | 1972 | 4631596 |
| the persistence of a microbial flora during postembryogenesis of drosophila melanogaster. | | 1969 | 4904970 |
| antibacterial compounds in the cell-free haemolymph of drosophila melanogaster. | | 1970 | 4907049 |
| persistence of lethal genes in japanese natural populations of drosophila melanogaster. | | 1970 | 4912302 |
| structure of drosophila melanogaster dat replicated in an in vitro system. | | 1970 | 4933684 |
| [selection of a mutant of the rabies virus adapted to an insect: drosophila melanogaster]. | | 1966 | 4961215 |
| [multiplication of the sindbis virus in drosophila melanogaster (dipterous insect) in experimental conditions]. | | 1967 | 4962181 |
| [repulsive action of living yeast on oviposition of drosophila melanogaster meig]. | | 1969 | 4976836 |
| [serologic comparison of drosophila melanogaster virus p and galleria mellonella densonucleosis virus]. | | 1970 | 4985243 |
| the genetics of aldehyde oxidase in drosophila melanogaster. | | 1970 | 4997169 |
| antigens of developing drosophila melanogaster. | | 1971 | 5000626 |
| [fertility and laying behavior in drosophila melanogaster: effect of several qualities of commercial yeast, of the volume of the cages and of the surface of the food]. | | 1971 | 5005125 |
| lethal mutations induced by dna in drosophila melanogaster. | | 1965 | 5214601 |
| a melting point for the birefringent component of muscle. | the a filament of the striated muscle sarcomere is an ordered aggregate of one or a few species of proteins. ordering of these filaments into a parallel array is the basis of birefringence in the a region, and loss of birefringence is therefore a measure of decreased order. heating caused a large decrease in the birefringence of glycerinated rabbit psoas muscle fibers over a narrow temperature range ( approximately 3 degrees c) and a large decrease in both the birefringence and optical density o ... | 1966 | 5339378 |
| sexual stimulation of males of lucilia cuprina (calliphoridae) and drosophila melanogaster (drosophilidae) by the odors of aggregation sites. | | 1969 | 5374179 |
| histones of polytene and nonpolytene nuclei of drosophila melanogaster. | | 1971 | 5547707 |
| antagonism of terramycin on action of bacillus thuringiensis 'exotoxin' in drosophila melanogaster. | | 1971 | 5549249 |
| the dna content of sperm of drosophila melanogaster. | | 1971 | 5574762 |
| histone acetylation in insect chromosomes. | acetylation of histones takes place along the salivary gland chromosomes of chironomus thummi when rna synthesis is active. it can be observed but not measured quantitatively by autoradiography of chromosome squashes. the "fixatives" commonly used in preparing squashes of insect chromosomes preferentially extract the highly acetylated "arginine-rich" histone fractions; the use of such fixatives may explain the reported absence of histone acetylation in drosophila melanogaster. | 1968 | 5634500 |
| spermatocyte granules in drosophila melanogaster. | | 1969 | 5797803 |
| differential induction of chromosome deletions by natural and synthetic macromolecules in drosophila melanogaster. | | 1965 | 5884228 |
| the expression of maternally-transmitted sex ratio condition (sr) in two strains of drosophila melanogaster. | | 1966 | 5965031 |
| [effect of cholesterase reactivators on diphosphorylated acetylcholinesterase from drosophila melanogaster meig. and from cattle erythrocytes]. | | 1966 | 5989983 |
| immunological evidence for structural homology between drosophila melanogaster (s14), rabbit liver (s12), saccharomyces cerevisiae (s25), bacillus subtilis (s6), and escherichia coli (s6) ribosomal proteins. | specific antibodies directed against drosophila melanogaster acidic ribosomal protein s14 were used in a comparative study of eucaryotic and procaryotic ribosomes by immunoblotting and enzyme-linked immunosorbent assays. common antigenic determinants and, thus, structural homology were found between d. melanogaster, saccharomyces cerevisiae (s25), rabbit liver (s12), bacillus subtilis (s6), and escherichia coli (s6) ribosomes. | 1984 | 6083442 |
| inducible transcription and puffing in drosophila melanogaster transformed with hsp70-phage lambda hybrid heat shock genes. | a series of hsp70-phage lambda hybrid genes having various amounts of 5' flanking dna was introduced into the germ line of drosophila melanogaster by p-element-mediated transformation. heat-induced transcription was normal in lines transformed with hsp70-lambda genes having 194 and 146 base pairs of dna upstream from the mrna initiation site. lines transformed with genes having 70 base pairs of upstream dna accumulated correctly initiated transcripts in response to heat shock, but the amount was ... | 1984 | 6089207 |
| supercoil-dependent features of dna structure at drosophila locus 67b1. | we have analyzed the pattern of supercoil-dependent, single strand-specific nuclease cleavage sites across 11.6 kb (11.6 x 10(3) base-pairs) of cloned drosophila melanogaster dna from locus 67b1. this region contains coding sequences for the heat shock proteins hsp23, hsp26 and hsp28 as well as for a 1.6 kb developmentally regulated transcript (r). two major sites are detected on digestion with s1 nuclease or mung bean nuclease. the most prominent site maps 100 base-pairs upstream of hsp26 in a ... | 1984 | 6090677 |
| genetic effects of injection of rous sarcoma virus dna into polar plasm of early drosophila melanogaster embryos. | retroviral proviruses and the transposable elements of eukaryotic genomes are structurally similar. the biological significance of eukaryotic transposable elements has not been examined extensively but it is known that, like prokaryotic transposons, these elements can induce mutations in adjacent genes and cause their transposition. it is of interest to determine whether retroviral proviruses have the same mutagenic and gene transposing ability as transposable elements, particularly because the ... | 1984 | 6090937 |
| detection of virus-specific sequences in drosophila melanogaster mutants induced by injection of rsv dna into early embryos. | the injection of purified rous sarcoma virus (rsv) (prague strain) into drosophila melanogaster (oregon r line) eggs changes the fly phenotype in certain cases, and rsv-specific sequences can be identified in the drosophila genome (ref. 1 and preceding paper). here we have used southern blotting to analyse in greater detail the proviral dna present in several mutant lines of d. melanogaster produced by microinjection of intact rsv or plasmid dna containing the viral insert. in certain population ... | 1984 | 6090938 |
| protein products of the bithorax complex in drosophila. | a sequence from the ubx 5' exon in the bithorax complex of drosophila melanogaster was expressed as a fusion protein in bacteria. this protein was used to raise rabbit antisera and monoclonal antibodies. these antibodies detect antigens that, on protein blots and by immunofluorescence on whole mounts of imaginal discs, show the predicted segmental distribution of ubx products. these products are predominantly, if not totally, localized in the cell nucleus. in the embryonic nervous system nuclei ... | 1984 | 6091908 |
| [induction of unstable mutations in drosophila melanogaster by the microinjection of oncogenic viruses and their dna into early embryos]. | rna-containing raus sarcoma virus, recombinant plasmid pbr322 inserted by kdna rsv (pprc11) and sa7 adenovirus dna were injected into the polar region of drosophila melanogaster early embryons. the exogenic genetic material injected was shown to induce mutations, many of them unstable. in a number of cases, virus-specific sequences were found in dna isolated from mutant flies. it is hypothesized that mutations induced by dna of oncogenic viruses are of the insertion type. | 1984 | 6092212 |
| 70-kilodalton heat shock polypeptides from rainbow trout: characterization of cdna sequences. | rtg-2 cells, a line of fibroblasts from rainbow trout (salmo gairdnerii), are induced to synthesize a distinct set of heat-shock polypeptides after exposure to elevated temperature or to low concentrations of sodium arsenite. we isolated and characterized two cdna sequences, ths70.7 and ths70.14, encoding partial information for two distinct species of 70-kilodalton heat shock polypeptide (hsp70) from these cells. these sequences are identical at 73.3% of the nucleotide positions in their region ... | 1984 | 6092938 |
| the role of connexon aggregate fusion in gap junction growth. | gap junctions are thought to grow in size by the incorporation of single connexons and by the fusion of connexon aggregates but the relative importance of these two growth mechanisms, particularly in the formation of large gap junctions, remains unclear. we have used a quantitative approach to distinguish between these two growth mechanisms and present evidence here that gap junctions in drosophila melanogaster wing discs have a periodic, (approximately equal to 0.025 micron 2), multimodal areal ... | 1984 | 6094843 |
| isolation and characterization of the beadex locus of drosophila melanogaster: a putative cis-acting negative regulatory element for the heldup-a gene. | we isolated recombinant lambda phage clones spanning 49 kilobases of dna which contain the beadex and heldup-a loci of drosophila melanogaster. these cloned dnas were used to analyze the structure of eight dominant mutant alleles of the beadex locus which show increased gene activity. a region, only 700 base pairs in length, is altered in each of these mutants. six of the mutations have dna insertions within this segment. most of these insertions resemble retrovirus-like transposable elements. i ... | 1984 | 6095064 |
| cloning of heat-shock locus 93d from drosophila melanogaster. | using the microcloning approach a number of recombinant lambda phages carrying dna from the 93d region have been isolated. screening genomic libraries, cloned in phage lambda or cosmid vectors, with this isolated dna yielded a series of overlapping dna fragments from the region 93d6-7 as shown by in situ hybridization to polytene chromosomes. in vitro 32p-labelled nuclear rna prepared from heat-shocked third instar larvae hybridized specifically to one fragment within 85 kb of cloned dna. the re ... | 1984 | 6096125 |
| structure of circular copies of the 412 transposable element present in drosophila melanogaster tissue culture cells, and isolation of a free 412 long terminal repeat. | we have isolated, from drosophila melanogaster tissue culture cells, extrachromosomal circular forms of the transposable element 412, and have cloned some of them in bacteriophage lambda. a total of 24 clones have been analysed in detail by restriction and heteroduplex mapping. seventeen clones are virtually identical, and contain complete 412 elements with one copy of the long terminal direct repeat (ltr). the remaining seven clones are all different and contain various rearrangements. four hav ... | 1984 | 6096559 |
| expression of a drosophila heat-shock gene in cells of the yeast saccharomyces cerevisiae. | a 3.52-kilobase (kb) segment of drosophila melanogaster dna carrying the 2.15-kb transcribed sequence for the 70 000-dalton heat-shock protein (hsp70) and 1.14-kb of the 5' flanking sequence was inserted into an autonomously replicating chimeric plasmid and used to transform the yeast saccharomyces cerevisiae. the drosophila gene is efficiently transcribed in the transformed cells, yielding a transcript which is 21 nucleotides shorter than the normal drosophila mrna at the 5' end. significant in ... | 1984 | 6098321 |
| changes in the abundance of polyadenylated rnas in development of drosophila melanogaster analyzed with cloned dna fragments. | | 1981 | 6113178 |
| characterization of an atpase/datpase activity associated with the drosophila nuclear matrix-pore complex-lamina fraction. identification of the putative enzyme polypeptide by direct ultraviolet photoaffinity labeling. | an atpase/datpase activity found associated with the nuclear matrix-pore complex-lamina fraction isolated from embryos of drosophila melanogaster has been characterized. in the presence of either ca2+ or mg2+, this activity hydrolyzed either atp or datp to adp or dadp, respectively, and pi. hydrolysis was optimal from ph 6.5-7.2, did not require either na+ or k+, and was not significantly inhibited by naf, ouabain, quercetin, na3vo4, ctp, or gtp. in contrast, hydrolysis was inhibited by n-ethylm ... | 1983 | 6131895 |
| drug metabolizing enzymes in drosophila melanogaster: teratogenicity of cyclophosphamide in vitro. | this report concerns the attempt to demonstrate the practicability of using microsomal fractions (rat or drosophila) for activating proteratogens in a drosophila in vitro teratogen screening assay. accordingly, a typical proteratogen (cyclophosphamide) was tested before and after microsomal activation and results compared with the drug directly on the in vitro assay. the effects of microsomal activation on known teratogens (thalidomide) and nonteratogens (acetaminophen) were also assayed in orde ... | 1983 | 6137081 |
| the rudimentary locus of drosophila melanogaster. | in the studies reported here, we have examined the molecular organization of the rudimentary gene of drosophila melanogaster. rudimentary encodes a 220,000 mr polypeptide catalyzing the first three steps in pyrimidine biosynthesis: carbamyl phosphate synthetase, aspartate transcarbamylase and dihydroorotase. we have determined the direction of transcription of the gene relative to the genetic map of the locus. the 5' end maps to the distal end of the locus (relative to the centromere) and transc ... | 1984 | 6144799 |
| structure, translation, and metabolism of the cytoplasmic copia ribonucleic acid of drosophila melanogaster. | we have characterized the copia rna in the cytoplasm of cultured drosophila cells. copia rna was detected and purified by hybridization to dna of the plasmid cdm 1142, which contains the copia sequence. a large fraction (2.2%) of the total cytoplasmic poly(a)+ rna was found to be copia rna. cytoplasmic copia rna displays all the characteristics expected for a messenger rna. it possesses a poly(a) tract identical in length with that of total poly(a)+ cytoplasmic rna. it is associated with polysom ... | 1980 | 6161644 |
| sequence of u1 rna from drosophila melanogaster: implications for u1 secondary structure and possible involvement in splicing. | u1 rna from cultured drosophila melanogaster cells (kc) was identified by its ability to be recognized, as an rnp, by anti-(u1)rnp antibodies from human lupus patients. its sequence was deduced largely from direct analysis of the rna molecule and then confirmed by dna sequence determinations on a genomic clone isolated by hybridization to drosophila u1 rna. the drosophila u1 rna sequence exhibits 72% agreement with human u1 rna. nucleotides 3-11, which are complementary to the entire consensus s ... | 1981 | 6172778 |
| mutagenic effects of bleomycin in drosophila melanogaster. | although bleomycin (blm) induces structural chromosome damage, eg, in human lymphocytes, no, or at best a weak, mutagenicity of this substance has been observed after the application of two well-established mutagenicity screening procedures, the ames test and the sister-chromatid exchange (sce) test. after feeding blm to drosophila melanogaster males we too, observed only a weak mutagenicity as measured by the frequency of recessive sex-linked lethal mutations. these results are based on the ana ... | 1980 | 6173212 |
| correlation of hnrnp structure and nascent transcript cleavage. | using electron microscopy of spread chromatin, we have observed nonnucleolar transcription units from drosophila melanogaster and calliphora erythrocephala that display specific cleavage of nascent transcripts. we have quantitatively analyzed 20 of these relatively long transcription units. the primary rnp structure of homologous transcripts is nonrandom with respect to both rna sequence and the cleavage event. in general, released rna fragments have a smooth fibrillar rnp morphology (approximat ... | 1981 | 6174239 |
| isolation and characterization of a proteinaceous subnuclear fraction composed of nuclear matrix, peripheral lamina, and nuclear pore complexes from embryos of drosophila melanogaster. | morphologically intact nuclei have been prepared from embryos of drosophila melanogaster by a simple and rapid procedure. these nuclei have been further treated with high concentrations of dnase i and rnase a followed by sequential extraction with 2% triton x-100 and 1 m nacl to produce a structurally and biochemically distinct preparation designated drosophila subnuclear fraction i (dsnf-i). as seen by phase-contrast microscopy, dsnf-i is composed of material which closely resembles unfractiona ... | 1982 | 6177701 |
| [induction of single- and double-strand breaks in linear and superhelical dna by phleomycin]. | phleomycin induced dna breakage was investigated with superhelical col. e1 dna and linear t2 dna as well. in both dna-forms besides single-strand breaks direct double-strand breaks were produced by the drug. the double-strand breakage rate obtained after treatment with phleomycin, however, was considerably smaller than that found after bleomycin treatment. whereas the single- and double-strand breakage rate in col. e1 dna showed a linear and nearly quadratic dependence on the phleomycin concentr ... | 1982 | 6190323 |
| isolation and characterization of drosophila melanogaster u2 small nuclear rna genes. | we describe here the organization of dna sequences complementary to drosophila melanogaster u2 small nuclear (sn) rna. from a genomic library we isolated two recombinants containing two genes each. genomic reconstruction experiments and southern analysis revealed that d. melanogaster possesses only four to five u2 snrna genes or very closely related sequences. the nucleotide sequence of one of the clones analysed shows 77% homology with rat u2 snrna. a stretch of 12 nucleotides that has been imp ... | 1983 | 6195341 |
| true genes for human u1 small nuclear rna. copy number, polymorphism, and methylation. | the nucleotide sequence of a human u1 small nuclear rna gene (hu1-1) is presented, along with several hundred nucleotides of its 5' and 3' flanking regions. we demonstrate that the immediate 5' flanking region sequences are a highly specific hydridization probe capable of distinguishing u1 rna true genes from pseudogenes. the copy number of u1 rna genes was determined to be constant, about 30/haploid genome equivalent in six different individuals. nevertheless, several restriction enzyme cleavag ... | 1984 | 6198328 |
| regulation of synthesis of the larval serum proteins of drosophila melanogaster. | we have determined the relative amounts of subunits of larval serum proteins (lsps) 1 and 2 during larval development in drosophila melanogaster. these results indicate that synthesis of polypeptide subunits of lsp-1 and lsp-2 is coordinate: the proteins are first detected at the same time; they accumulate in a coordinate fashion; their rnas are first detected at the same time; the rnas also accumulate in similar relative amounts. analyses of fat body polypeptides and fat body rna indicate that ... | 1984 | 6199241 |
| proteins related to the mouse l-cell major heat shock protein are synthesized in the absence of heat shock gene expression. | heat shock of mouse l cells induces the synthesis of two polypeptides of mrs 68,000 and 89,000. using a fragment of a cloned gene encoding the drosophila melanogaster mr 70,000 heat shock protein (hsp70), we have shown that this protein has been highly conserved during eukaryotic evolution. we extended this observation by probing at low stringency for the expression in mouse l cells of rna homologous to the drosophila hsp70 gene. in addition to the rna encoding the inducible mr 68,000 heat shock ... | 1984 | 6201852 |
| mobile genetic elements in animal cells and their biological significance. | mobile genetic elements were discovered by mcclintock while analysing unstable mutations in maize. the structural and functional studies of such elements became possible after their cloning, first from the genome of drosophila melanogaster. in particular, ilyin et al. demonstrated the varying location of the described elements in d. melanogaster chromosomes, thus providing the first evidence of their mobility. mobile elements comprise a significant part of the genetic material in d. melanogaster ... | 1984 | 6209137 |
| alu sequences are processed 7sl rna genes. | 7sl rna is an abundant cytoplasmic rna which functions in protein secretion as a component of the signal recognition particle. alu sequences are the most abundant family of human and rodent middle repetitive dna sequences (reviewed in ref. 2). the primary structure of human 7sl rna consists of an alu sequence interrupted by a 155-base pair (bp) sequence that is unique to 7sl rna. in order to obtain information about the evolution of the alu domain of 7sl rna, we have determined the nucleotide se ... | 1984 | 6209580 |
| expression and novel structure of a collagen gene in drosophila. | we report the structure and developmental expression of collagen gene sequences in drosophila melanogaster. collagen-like genomic clones were isolated by screening a drosophila genomic library with a chicken pro alpha 2(i) cdna clone as a hybridization probe. a 1.5-kilobase (kb) dna sequence from a 9.2-kb dna clone (pdcg1) is presented. unlike the highly fragmented genes for vertebrate type i collagen, there is no evidence of a 54-base-pair primordial unit within this gene segment. instead, the ... | 1982 | 6210912 |
| latitudinal relationships of esterase-6 and phosphoglucomutase gene frequencies in drosophila melanogaster. | geographic variation in esterase-6 (est-6) and phosphoglucomutase (pgm) gene frequencies in australasian populations of drosophila melanogaster are compared with analogous data collated from 16 previous reports for north america and europe/asia. a large-scale latitudinal cline is found on all three zoogeographic zones for est-6 and overall, est-61.00 frequency increases from about 20 per cent around 20 degrees latitude to about 80 per cent approaching 50 degrees latitude. in contrast, there is n ... | 1981 | 6211421 |
| estimation of the amount of 5-methylcytosine in drosophila melanogaster dna by amplified elisa and photoacoustic spectroscopy. | we have previously reported a sensitive immunochemical method for detecting 5-methylcytosine in dna which involves spotting dna samples on nitrocellulose paper and detection of 5-methylcytosine, if any, by a combination of the double antibody method and a staining reaction brought about by biotin-avidin and peroxidase. we report here a linear relationship between the concentration of 5-methylcytosine in dna and staining intensity, as recorded by photoacoustic spectroscopy. it appears possible to ... | 1984 | 6232132 |
| the dna polymerase-primase from drosophila melanogaster embryos. rate and fidelity of polymerization on single-stranded dna templates. | the dna polymerase activity of the near homogeneous, multisubunit dna polymerase-primase from drosophila melanogaster embryos has been compared to escherichia coli dna polymerase iii core, dna polymerase iii, and dna polymerase iii holoenzyme. the rate of deoxynucleotide incorporation by the drosophila polymerase on singly primed phi x174 dna is similar to that observed with equivalent levels of dna polymerase iii holoenzyme in the absence of e. coli single-stranded dna binding protein. however, ... | 1984 | 6235226 |
| vesicular stomatitis virus growth in drosophila melanogaster cells: g protein deficiency. | in cultured drosophila melanogaster cells, vesicular stomatitis virus (vsv) established a persistent, noncytopathic infection. no inhibition of host protein synthesis occurred even though all cells were initially infected. no defective interfering particles were detected, which would explain the establishment of the carrier state. in studies of the time course of viral protein synthesis in drosophila cells, n, ns, and m viral polypeptides were readily detected within 1 h of infection. the yield ... | 1980 | 6245242 |
| construction of phylogenetic trees by pattern recognition procedures. | the sequence of a protein can be graphed as a discrete function and a cross-correlation between any two such number sets produces a similarity score. the scores are used to prepare a phylogenetic tree involving hierarchical cluster analysis, non-linear mapping, and minimal spanning routines. changes are suggested in the sequences of cytochrome c's from mediterranean fruit fly, locust, and rattlesnake. the method is faster than existing procedures and does not require human intervention at any st ... | 1980 | 6250297 |
| distribution of sequences common to the 25--28s-ribonucleic acid genes of xenopus laevis and neurospora crassa. | the extent of homology between the nucleotide sequence of l-rrna (the major rna component of the larger ribosomal subparticle) of a lower eukaryote (neurospora crassa) and an amphibian (xenopus laevis) was investigated by utilizing rdna (dna coding for rrna) of x. laevis cloned in plasmids pmb9 and pml2, and rdna of n. crassa cloned in bacteriophage lambda. hybridization studies revealed that sequences common to both n. crassa and x. laevis l-rrna comprise a total of approx. 1050 /+- 200 nucleot ... | 1980 | 6250536 |
| sequence of the 3'-terminal portion of drosophila melanogaster 18 s rrna and of the adjoining spacer: comparison with corresponding prokaryotic and eukaryotic sequences. | | 1980 | 6250894 |
| comparative analysis of proteins selectively released from cell nuclei of different eukaryotic species under conditions of mild hydrolysis by nucleases. | nuclei of cultured chinese hamster fibroblasts, dechoryonized eggs of drosophila melanogaster, calf thymocytes and mouse spleen were digested with micrococcal nuclease or dnase i under conditions when about 15-20% of nuclear dna is hydrolyzed. proteins released from the nuclei with this dna were analyzed by electrophoresis in polyacrylamide gels in the presence of sds and the most prominent components were purified in each case. it is shown that these components derived from different species ha ... | 1980 | 6251362 |
| [simple method of cloning eukaryote dna: production of certain new ribosomal genes of drosophila]. | we propose a simple method which allows to receive a collection of clones containing recombinant plasmids. it is based on the ligation of the longer fragment of pbr332 formed by ecori and bamh1 with eukaryotic dna (from drosophila melanogaster embryo in this case) partially cleaved with ecori and bamhi. this approach gave us 10(4) colonies from 1 microgram of drosophila dna and 0.1 microgram of the bamhi--ecori "vector". about 0.5% of all clones carried the fragments of ribosomal genes with inse ... | 1980 | 6252449 |
| putative actin genes in the macronucleus of oxytricha fallax. | previous work has shown that the macronuclear dna of the hypotrichous ciliate oxytricha fallax is arranged as short achromosomal pieces, 22 to 0.5 kilobase pairs (kb) in length. micronuclear dna has a typical chromosomal organization. macronuclear dna is derived from micronuclear dna through a process of polytene chromosome fragmentation with a resultant decrease in dna sequence complexity. three putative actin genes have been identified in macronuclear dna by using a cloned yeast actin gene as ... | 1980 | 6254076 |
| four heat shock proteins of drosophila melanogaster coded within a 12-kilobase region in chromosome subdivision 67b. | unique coding sequences for four heat shock proteins of drosophila melanogaster, hsp 28, hsp 26, hsp 23, and hsp 22, are clustered in a 12-kilobase interval at chromosome subdivision 67b. the four genes are not transcribed in the same direction and each gives rise to a separate messenger rna, with no indication of intervening sequences. including the present results, the genes for all seven major heat shock proteins of d. melanogaster are now cloned are found to exhibit a variety of patterns of ... | 1980 | 6254079 |
| [reiterated genes scattered among the chromosomes of drosophila melangoaster with varying locations. v. nature of self-complementary rna sequences transcribed from chromosomal dna]. | double-stranded rna (dsrna) sequences were isolated from either total rna or cytoplasmic poly(a)+rna of drosophila melanogaster culture cells. virtually all dsrna was found to be of high molecular weight (> 200 base pairs) and unable to snap back after rna melting. thus, it corresponds to one of dsrna classes found in mouse cells, namely, to dsrna-a. three different cloned dna fragments of d. melanogaster which hybridized to melted dsrna were selected among 100 randomly taken. all of them effici ... | 1980 | 6256262 |
| a new high-capacity cosmid vector and its use. | the construction of a cosmid, mua-3, designed for the convenient cloning of eukaryotic dna segments up to 48 kb in length is described. the cosmid contains all of the plasmid pbr322 with approx. 400 bases of lambda dna, including the cohesive end site, inserted at the pbr322 psti endonuclease recognition site. methods for using this vector to construct several types of drosophila melanogaster genomic dna libraries are given, and libraries made by these methods are characterized. a sheared drosop ... | 1980 | 6260574 |
| [instability of hybrid plasmids containing drosophila melanogaster dna in rec+ and rec- escherichia coli k-12 strains]. | the stability of hybrid plasmids, constructed on the basis of vector pcv20(aprtcr) and containing hindiii fragments of drosophila melanogaster dna (pdm6, pdm9) and psti fragments of d. melanogaster dna (pdm39, pdm187, pdm189) was studied. after the transformation of e. coli hb101 reca and escherichia coli 802 rec+ and selection to tcr (pdm6, pdm9), or to apr (pdm39, pdm189, pdm187) 0.04--9% of clones with reduced resistance to tc or ap was detected. the hybrid plasmids are more stable in rec-, b ... | 1981 | 6262192 |
| localization of arginine kinase in muscles fibres of drosophila melanogaster. | arginine kinase (ak), a monomeric protein of mol. wt. 40 000, was purified from adult drosophila melanogaster. antiserum to ak was raised in rabbits; its specificity was established by immunodiffusion tests and by immunoreplicas of electropherograms. after electrophoresis in non-denaturing conditions, the immunoreactive material was shown to possess enzymatic activity. localization of ak by indirect immunofluorescence on washed myofibrils showed that at least a fraction of the total cellular ak ... | 1980 | 6262374 |
| nucleotide sequence of the initiation site for ribosomal rna transcription in drosophila melanogaster: comparison of genes with and without insertions. | the sequence of 470 nucleotides surrounding the initiation site for rrna transcription in drosophila melanogaster has been determined. the precise initiation site was determined first by measuring the dna fragment protected by the rrna precursor against digestion by the single-strand specific nuclease s1 and second by direct sequence determination of the first 13 nucleotides of the rrna precursor. because greater than 80% od rrna precursor molecules have been shown previously to bear pppa or ppa ... | 1981 | 6262809 |
| a calcium/calmodulin-dependent cyclic adenosine monophosphate phosphodiesterase from drosophila heads. | a ca2+-activated cycl amp phosphodiesterase from drosophila melanogaster heads was studied. the enzyme accounted for approx. 40% of the total, soluble cyclic amp phosphodiesterase activity in heads. after gel filtration, ca2+ stimulation of the enzyme was no longer apparent, but ca2+ activation could be restored by the addition of boiled drosophila extract to the column-fractionated phosphodiesterase. the protein responsible for restoring ca2+ activation was purified and shown to have some chara ... | 1981 | 6263352 |
| the nucleotide sequence at the transcription termination site of ribosomal rna in drosophila melanogaster. | the sequence of 300 nucleotides surrounding the transcription termination site for ribosomal rna in drosophila melanogaster has been determined. the precise position of the rna 3'-end was located by sizing rdna fragments protected from s1 nuclease by hybridization with cytoplasmic 28s rrna or with nuclear 38s pre-rrna. next, the sequence of the first 10 nucleotides at the 3'-end of 28s and 38s rrna was determined directly. the results indicate the absence of 3'-terminal processing of the 38s pre ... | 1981 | 6264393 |
| isolation and characterization of human actin genes. | we have utilized cloned actin genes from drosophila melanogaster and from chicken to isolate 12 actin gene fragments from a human dna library. each of these 12 clones was shown to contain actin coding regions by its ability to selectively hybridize to human actin mrna as assayed by in vitro translation. the translation product was judged to be actin on the basis of its comigration with authentic actins when electrophoresed on one- and two-dimensional nadodso4/polyacrylamide gels and on the basis ... | 1981 | 6272269 |
| integration, transcription, and control of a drosophila heat shock gene in mouse cells. | mouse l cells were transformed with a cloned 3.6-kilobase (kb) segment of drosophila melanogaster dna carrying the 2.25-kb transcribed sequence for the drosophila 70,000-dalton heat shock protein (hsp70) and 1.1 kb and 0.2 kb of 5' and 3' flanking dna, respectively. heat shock of one of three such transformed cell lines containing multiple copies of the intact drosophila segment induced the abundant accumulation of transcripts of the drosophila gene, with correct or nearly correct 5' and 3' term ... | 1981 | 6273904 |
| nucleotide sequence of the 3'-terminal region of rat 18s ribosomal dna. | the 3'-terminal 230 base-pairs (bp) of the gene for 18s rrna and 40 bp of the adjoining spacer have been sequenced for the sprague-dawley rat. this mammalian sequence has been compared with the known sequences of yeast, fruit fly, silkworm, and frog. this study has shown that the nucleotide-sequence differences between rat and frog are the smallest among and longer species, probably reflecting their evolutionary closeness and longer maturation time compared to the others. there is little similar ... | 1981 | 6278265 |
| in vitro transcription of a cloned mouse ribosomal rna gene. | an in vitro transcription system which utilizes cloned mouse ribosomal rna gene (rdna) fragments and a mouse cell extract has been developed. rna polymerases i is apparently responsible for this transcription as evidenced by the complete resistance to a high concentration (200 micrograms/ml) of alpha-amanitin. run-off products obtained with three different truncated rdna fragments indicated that rna was transcribed from a unique site of rdna. the s1 nuclease protection mapping of the in vitro pr ... | 1981 | 6278446 |
| glutamate trna genes are adjacent to 5s rna genes in drosophila and reveal a conserved upstream sequence (the act-ta box). | in drosophila melanogaster at least six transfer rna genes are located adjacent to the 3' end of the 5s rna gene cluster. three of these have been sequenced and identified as coding for glutamate trna4. in the chromosome they are arranged as tandem repeats on the same dna strand and transcribed in the same direction as is 5s dna, towards the centromere. we have also identified a sequence, the act-ta box, that is highly conserved among the polymerase iii transcribed genes. usually the sequence is ... | 1982 | 6289265 |
| immunoglobulin switch region-like sequences in drosophila melanogaster. | we found immunoglobulin switch (s) region-like sequences in dnas of wide variety of organisms including sea urchin, yeast and drosophila that do not produce immunoglobulins. dna fragments carrying smu-like sequences were cloned from drosophila and the nucleotide sequence of a clone is almost identical to that of the mouse smu region. restriction fragments of drosophila smu-like sequences and their flanking regions seem to vary among drosophila species. possible evolutionary significance of the s ... | 1982 | 6289266 |
| the proteins expressed by different isolates of drosophila c virus. | isolates of drosophila c virus (dcv) from drosophila flies obtained in geographically different regions were adapted to growth in drosophila tissue culture cells. the viruses, purified from tissue culture cells, were shown to be serologically related to one of the isolates ("o" from ouarzazate, morocco). analysis of the structural proteins by polyacrylamide gel electrophoresis demonstrated differences between the isolates. labelling intracellular proteins of infected drosophila melanogaster cell ... | 1982 | 6297428 |
| homology between actin coding and its adjacent sequences in widely divergent species. | eco ri restriction endonuclease dna fragments from several representatives of the kingdoms protista and animalia were electrophoretically separated and transferred to the nitrocellulose filters. these dna's were hybridized with [32p]-labelled actin coding sequence from drosophila melanogaster (dm). the results indicate that the nucleic acid sequences of the genes coding for actin(s) has been highly conserved throughout evolution. similar experiments were performed using the sequence derived from ... | 1983 | 6299292 |
| sequence of a drosophila dna segment that functions in saccharomyces cerevisiae and its regulation by a yeast promoter. | we have determined the complete dna sequence of a segment derived from drosophila melanogaster that complements a yeast adenine-8 (ade8) mutation. this sequence, in conjunction with transcriptional analysis, predicts that a 206 amino acid sequence accounts for complementation. we have ligated a 1.5 kb promoter segment of the yeast alcohol dehydrogenase i (adhi) gene just upstream of the open reading frame. this construction results in ade8+ function that responds to glucose and ethanol in a mann ... | 1983 | 6300768 |
| one side of a deletion breakpoint from the drosophila melanogaster genome contains a transposable element. | the deletion-fusion fragment of the drosophila melanogaster x-chromosome deficiency df(1)n5419 was cloned in a lambda phage vector. dna on the genetic left side of the fusion contains sequences homologous to the roo family of transposable repetitive elements. restriction mapping data are consistent with the right end of the roo element being situated near the breakpoint. the deficiency is of spontaneous origin, and it is possible that transposition of the roo element was involved in the deletion ... | 1983 | 6301953 |
| a dna-binding protein from ustilago maydis prefers duplex dna without chain interruptions. | using a nitrocellulose filter binding assay, we have partially purified a protein from mitotic cells of ustilago maydis that binds preferentially to covalently closed circular duplex dna. dna containing single- or double-strand breaks is bound poorly by the protein. once formed, the dna-protein complex is stable, resisting dissociation in high salt. however, when a dna strand is broken, the complex appears to dissociate. the protein binds equally well to form i dna of phi x174 or the plasmid pbr ... | 1983 | 6304499 |
| close relationship between the long terminal repeats of avian leukosis-sarcoma virus and copia-like movable genetic elements of drosophila. | a new species of copia-like movable genetic element termed 17.6 was identified in drosophila melanogaster, and the nucleotide sequences of its long terminal repeats (ltrs) were determined. the ltrs of 17.6 were not only homologous to those of 297, a sibling movable genetic element of 17.6, but also closely matched those of avian leukosis-sarcoma virus. this made it possible (i) to identify the nucleotide sequences in 17.6 and 297 that correspond to the crucial regulatory sequences for both trans ... | 1983 | 6304696 |
| knotting of the circular duplex dna by type ii dna topoisomerase from drosophila melanogaster. | dna molecules with topological knots can be detected in a reaction mixture of circular dna and type ii dna topoisomerase purified from the embryos of drosophila melanogaster. similar to the dna knotting reaction by bacteriophage t4-induced topoisomerase (liu, l. f., liu, c. c., and alberts, b. m. (1980) cell 19, 697-707), the knotting reaction by the drosophila topoisomerase ii requires a high ratio of enzyme/dna. however, they differ in the following aspects: 1) the knotting reaction by the dro ... | 1983 | 6305983 |
| double strand dna cleavage by type ii dna topoisomerase from drosophila melanogaster. | the purified type ii dna topoisomerase from the embryos of drosophila melanogaster exists in its native form as a dimer of 170,000-dalton polypeptides. in addition to the 170,000-dalton polypeptides, 3 polypeptides with molecular weights of 151,000, 141,000, and 132,000 were resolved when the enzyme was analyzed by electrophoresis under denaturing conditions. all four polypeptides can participate in the topoisomerase cleavage reaction and form covalent complexes with the cleaved dna. furthermore ... | 1983 | 6305984 |
| escherichia coli single-strand binding protein stabilizes specific denatured sites in superhelical dna. | escherichia coli single-strand binding protein relaxes supercoiled dna molecules containing the drosophila melanogaster histone gene repeat unit, by stabilizing denaturation bubbles that map near the boundaries of the genes, at sites that in native chromatin have been shown to be hypersensitive to nucleases. a similar process may contribute to the propagation of such hypersensitive sites after their induction on the activation of gene expression. | 1983 | 6306467 |
| vesicular stomatitis virus growth in drosophila melanogaster cells. ii. modifications of viral protein phosphorylation. | the phosphoproteins of vesicular stomatitis virus released from infected drosophila melanogaster cells were examined. the membrane (m) protein was more phosphorylated than after multiplication in chicken embryo cells, even in drosophila cell cytoplasm before its association with cellular membranes. analysis of phosphopeptides generated after partial proteolysis and of phosphoamino acids obtained after complete acid hydrolysis showed that m phosphorylation was quantitatively and qualitatively cha ... | 1983 | 6308137 |
| the effect of two chemical mutagens enu and mms on mr-mediated reversion of an insertion-sequence mutation in drosophila melanogaster. | the effects of two mutagens enu and mms characterized by different alkylation patterns have been studied on the reversion of an mr-induced singed mutation to wild-type. reversion of this unstable singed mutation under the influence of mr is assumed to represent the removal or transposition of an insertion element. since mr acts primarily in spermatogonia, the mutagens were fed to 1st instar larvae. recessive lethal tests were carried out simultaneously to calibrate for the mutagenic effectivenes ... | 1983 | 6308435 |
| a yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product. | organisms amenable to easy genetic analysis should prove helpful in assessing the function of at least those proto-oncogene products which are highly conserved in different eukaryotic cells. one obvious possibility is to pursue the matter in drosophila melanogaster dna, which has sequences homologous to several vertebrate oncogenes. another is to turn to the yeast saccharomyces cerevisiae, if it contains proto-oncogene sequences. here we report the identification of a gene in s. cerevisiae which ... | 1983 | 6318115 |
| ras-related gene sequences identified and isolated from saccharomyces cerevisiae. | the oncogenes of harvey and kirsten murine sarcoma viruses (v-rash and v-rask) and their cellular homologues (c-rash and c-rask) constitute two members of the ras gene family. each functional member of the ras gene family encodes a 21,000 molecular weight protein (p21ras). ras genes have been detected in a wide variety of vertebrate species, including xenopus laevis (r. e. steele, personal communication), and in drosophila melanogaster. we report here the detection of ras-related genes in the ye ... | 1983 | 6318116 |
| the normal developmental regulation of a cloned sgs3 'glue' gene chromosomally integrated in drosophila melanogaster by p element transformation. | a 7-kb genomic segment containing the coding sequence for the drosophila melanogaster formosa variant of salivary gland secretion protein 3 (sgs3) has been inserted into the snw y, bw, st strain of d. melanogaster using the p transformation vector p.6.1. the inserted sequence codes for a shorter rna which can be distinguished from that of the host gene. the amount of rna, and its stage- and tissue-specific synthesis is identical to that of the host gene, which suggests that all the cis-acting re ... | 1983 | 6321149 |
| isolation and characterization of an alpha-tubulin gene from leishmania enriettii. | an alpha-tubulin gene of leishmania enriettii has been identified in genomic southern blots by hybridization with a heterologous alpha-tubulin gene from drosophila melanogaster. a clone containing this gene has been isolated from a plasmid library of size-selected l. enriettii dna. it was identified by hybridization with the d. melanogaster tubulin gene. the cloned dna fragment was characterized by restriction analysis and partial dna sequence analysis. the cloned dna fragment is 2 kb in length, ... | 1983 | 6321982 |