| leishmania infantum dna detected in phlebotomine species from puerto iguazú city, misiones province, argentina. | in puerto iguazú city, argentina, human and canine visceral leishmaniasis cases have been recorded since the year 2010, with leishmania infantum as the etiological agent and lutzomyia longipalpis as its main vector. in the present study, polymerase chain reaction and sequencing were used to detect l. infantum dna in 3.9% of the female sandflies captured in puerto iguazú city. this is the first report of l. infantum dna detection in micropygomyia quinquefer, and the second one in lu. longipalpis ... | 2017 | 28476601 |
| occurrence and probability maps of lutzomyia longipalpis and lutzomyia cruzi (diptera: psychodidae: phlebotominae) in brazil. | leishmaniases are serious diseases caused by trypanosomatid protozoans of the genus leishmania transmitted by the bite of phlebotomine sand flies. we analyzed records pertaining to lutzomyia longipalpis (lutz and neiva, 1912) and lutzomyia cruzi (mangabeira, 1938) in brazil from the following sources: the collection of phlebotomine sand flies of the centro de pesquisas rené rachou/fiocruz (fiocruz-colfleb), the "specieslink" (cria) database, from systematic surveys of scientific articles and gra ... | 2017 | 28472338 |
| standardization of a continuous assay for glycosidases and its use for screening insect gut samples at individual and populational levels. | glycoside hydrolases (ghs) are enzymes able to recognize and cleave glycosidic bonds. insect ghs play decisive roles in digestion, in plant-herbivore, and host-pathogen interactions. gh activity is normally measured by the detection of a release from the substrate of products as sugars units, colored, or fluorescent groups. in most cases, the conditions for product release and detection differ, resulting in discontinuous assays. the current protocols result in using large amounts of reaction mix ... | 0 | 28553236 |
| polymerase chain reaction-based assay for the detection and identification of sand fly gregarines in lutzomyia longipalpis, a vector of visceral leishmaniasis. | gregarines that parasitise phlebotomine sand flies belong to the genus psychodiella and, even though they are highly host-specific, only five species have been described to date. their most outstanding features include the unique localisation of the oocysts in the accessory glands of the female host, which ensures contamination of the egg surface during oviposition, and the fact that they naturally parasitise the vectors of leishmania, causal agent of leishmaniasis. the type species, ps. chagasi ... | 2014 | 24820560 |
| ph control in the midgut of aedesaegypti under different nutritional conditions. | aedes aegypti is one of the most important disease vectors in the world. because their gut is the first site of interaction with pathogens, it is important to understand a. aegypti gut physiology. in this study, we investigated the mechanisms of ph control in the midgut of a. aegypti females under different nutritional conditions. we found that unfed females have an acidic midgut (ph ∼6). the midgut of unfed insects is actively maintained at ph 6 regardless of the ingestion of either alkaline or ... | 2017 | 28931720 |
| the role of gallery forests in maintaining phlebotominae populations: potential leishmania spp. vectors in the brazilian savanna. | knowledge on synanthropic phlebotomines and their natural infection by leishmania is necessary for the identification of potential areas for leishmaniasis occurrence. | 2017 | 28953996 |
| spatial population dynamics and temporal analysis of the distribution of lutzomyia longipalpis (lutz & neiva, 1912) (diptera: psychodidae: phlebotominae) in the city of clorinda, formosa, argentina. | lutzomyia longipalpis, the vector for the causal agent of visceral leishmaniasis (vl), has extended its distribution in the southern cone in the americas. the first urban record of lu. longipalpis in argentina was from the city of clorinda in 2004. the aim of this study was to analyse the monthly distribution and abundance of lu. longipalpis and to evaluate its association with environmental and climatic variables in clorinda city, province of formosa. | 2017 | 28743283 |
| ultrastructure of male sex pheromone glands in abdominal tergites of five lutzomyia sandfly species (diptera: psychodidae). | the fine structure of male sex pheromone disseminating structures on abdominal segments of five species of lutzomyia sandflies (diptera: psychodidae) was analyzed. scanning electron microscopy showed that in lutzomyia cruzi [mem. inst. oswaldo cruz 33 (1938) 349] and lutzomyia longipalpis [mem. inst. oswaldo cruz 4 (1912) 84], the disseminating structures are located in pale spots on abdominal tergites iv or iii and iv and are morphologically similar, appearing as small round cuticular elevation ... | 2002 | 18088957 |
| new records of phlebotomine sand flies (diptera: psychodidae) from the state of alagoas, northeast of brazil. | phlebotomine sand flies are small insects of great medical importance. this study presents new records of phlebotomine sand flies, which augment the list of species, recorded in the state of alagoas, brazil. sand flies were collected using cdc miniature light traps. collections were made between november 2008 and april 2016, in 47 municipalities within the state of alagoas. twenty-seven thousand two-hundred forty-four sand flies were collected, representing 18 species in seven genera. lutzomyia ... | 2018 | 29029319 |
| visceral leishmaniasis in an environmentally protected area in southeastern brazil: epidemiological and laboratory cross-sectional investigation of phlebotomine fauna, wild hosts and canine cases. | leishmaniasis is a rapidly expanding zoonosis that shows increasing urbanization. concern exists regarding the role of wildlife in visceral leishmaniasis (vl) transmission, due to frequent natural or anthropogenic environmental changes that facilitate contact between wildlife, humans and their pets. the municipality of campinas, in southeastern brazil, initially recorded vl in 2009, when the first autochthonous case was confirmed in a dog living in an upscale residential condominium, located ins ... | 2017 | 28704391 |
| the role of wing geometric morphometrics in the identification of sandflies within the subgenus lutzomyia. | the lutzomyia subgenus (diptera: psychodidae) includes sibling species with morphologically indistinguishable females. the aims of this study were to analyse variations in the size and shape of wings of species within the lutzomyia subgenus and to assess whether these analyses might be useful in their identification. wings (n = 733) of 18 species deposited in brazilian collections were analysed by geometric morphometrics, using other genera and subgenera as outgroups. shape variation was summari ... | 2017 | 28707301 |
| morphometric analysis of longipalpis (diptera: psychodidae) complex populations in mato grosso do sul, brazil. | owing to the existence of cryptic species that are difficult to distinguish morphologically, the search for new taxonomic characters and methods for identifying and classifying sand flies continues. lutzomyia longipalpis (lutz & neiva, 1912) and lutzomyia cruzi (mangabeira, 1938) (diptera: psychodidae) are two such species that occur in sympatry in some regions of mato grosso do sul state (ms). twenty females and twenty males from each of the five populations of lu. longipalpis and one populatio ... | 2015 | 26334809 |
| phlebotomine fauna, natural infection rate and feeding habits of lutzomyia cruzi in jaciara, state of mato grosso, brazil. | visceral leishmaniasis (vl) in brazil is transmitted by the phlebotomine lutzomyia longipalpis and in some midwestern regions by lutzomyia cruzi. studies of the phlebotomine fauna, feeding habits and natural infection rate by leishmania contribute to increased understanding of the epidemiological chain of leishmaniases and their vectorial capacity. collections were performed in jaciara, state of mato grosso from 2010-2013, during which time 2,011 phlebotomines (23 species) were captured (68.70% ... | 2014 | 25338156 |
| phlebotomine sandflies fauna (diptera: psychodidae) at rural settlements in the municipality of cáceres, state of mato grosso, brazil. | leishmaniasis is expanding across brazil, including the state of mato grosso (mt). the aim of this study was to characterize the phlebotomine sandfly fauna at threerural settlements located in the municipality of cáceres, mt, from august 2010 to july 2011. | 2012 | 22836661 |
| sandflies (psychodidae: phlebotominae) survey in an urban transmission area of visceral leishmaniasis, northeastern brazil. | visceral leishmaniasis (vl) is a major public health challenge in brazil, especially in states where it is endemic. the aim of this study was to investigate the relationship of sand fly population density with environmental variables (temperature, rainfall and relative humidity) in urban areas of the city of mossoró, rio grande do norte, northeastern brazil. sand flies were captured with center disease control (cdc) traps installed monthly in the intra and peridomicile of three houses. data anal ... | 2010 | 21184700 |
| bionomic aspects of lutzomyia evansi and lutzomyia longipalpis, proven vectors of leishmania infantum in an endemic area of non-ulcerative cutaneous leishmaniasis in honduras. | some lutzomyia species are the vectors of human leishmaniasis in the americas. visceral and cutaneous leishmaniasis are both endemic in the pacific region of honduras, but the non-ulcerative form is the more frequent clinical manifestation in this region, where lutzomyia longipalpis is the most abundant and the only incriminated vector. taxonomic identification and distribution studies of sand flies are important to understand the epidemiology and to control these neglected tropical diseases. | 2018 | 29304878 |
| nestedness patterns of sand fly (diptera: psychodidae) species in a neotropical semi-arid environment. | a common pattern in neotropical leishmania spp. transmission is the co-occurrence of several sand fly (sf) species at endemic foci. we collected 13 sf spp. by direct aspiration in natural resting places (nrp) and 10 sf spp. with shannon traps (st), totaling 15 spp. with both methods, at 6 locations within a semi-arid region with endemic visceral leishmaniasis transmission in falcón state, northwestern venezuela. we used null model testing of species co-occurrence and nestedness metrics estimated ... | 2016 | 26456179 |
| distribution of putative male sex pheromones among lutzomyia sandflies (diptera: psychodidae). | male lutzomyia longipalpis produce terpene sex pheromones in glandular tissue underlying the cuticle. the pheromones are transmitted to the surface via cuticle-lined ducts (measuring 0.25 microm in diameter), each of which reaches the surface in the centre of a papule (measuring 3-3.5 microm in diameter). similar papules, in a range of shapes but all characterized by the presence of a central pore and absence of macroserae, occur in some other species of sandfly. the aim of the present study was ... | 2002 | 11989537 |
| molecular detection of leishmania (leishmania) infantum in phlebotomine sandflies from a visceral leishmaniasis endemic area in northwestern of são paulo state, brazil. | this study identified the natural infection rate of leishmania (leishmania) infantum in lutzomyia longipalpis sandflies collected in a neighborhood around a kennel, in dracena, northwestern of são paulo state. this region is highly endemic for visceral leishmaniasis in brazil. insects were captured during 2 to 3 nights monthly for 11 months (january-november 2012) using 10 automatic light traps around a kennel in a transition between periurban and urban neighborhood. capture aimed the determinat ... | 2018 | 29373820 |
| identification of secreted proteins involved in nonspecific dsrna-mediated lutzomyia longipalpis ll5 cell antiviral response. | hematophagous insects transmit infectious diseases. sand flies are vectors of leishmaniasis, but can also transmit viruses. we have been studying immune responses of lutzomyia longipalpis, the main vector of visceral leishmaniasis in the americas. we identified a non-specific antiviral response in l. longipalpis ll5 embryonic cells when treated with non-specific double-stranded rnas (dsrnas). this response is reminiscent of interferon response in mammals. we are investigating putative effectors ... | 2018 | 29346269 |
| masp-1 of the complement system enhances clot formation in a microvascular whole blood flow model. | the complement and coagulation systems closely interact with each other. these interactions are believed to contribute to the proinflammatory and prothrombotic environment involved in the development of thrombotic complications in many diseases. complement masp-1 (mannan-binding lectin-associated serine protease-1) activates coagulation factors and promotes clot formation. however, this was mainly shown in purified or plasma-based static systems. here we describe the role of masp-1 and complemen ... | 2018 | 29324883 |
| antibody response to sand fly saliva is a marker of transmission intensity but not disease progression in dogs naturally infected with leishmania infantum. | antibody responses to sand fly saliva have been suggested to be a useful marker of exposure to sand fly bites and leishmania infection and a potential tool to monitor the effectiveness of entomological interventions. exposure to sand fly bites before infection has also been suggested to modulate the severity of the infection. here, we test these hypotheses by quantifying the anti-saliva igg response in a cohort study of dogs exposed to natural infection with leishmania infantum in brazil. | 2018 | 29301571 |
| bacterial diversity of wild-caught lutzomyia longipalpis (a vector of zoonotic visceral leishmaniasis in brazil) under distinct physiological conditions by metagenomics analysis. | the leishmaniases are a group of diseases caused by protozoans of the genus leishmania, which are transmitted by the bite of phlebotomine sand flies. in the new world, lutzomyia longipalpis is the most important vector of visceral leishmaniasis and is a proven vector for leishmania infantum chagasi in brazil. during development within the vector, leishmania can interact with a variety of microorganisms such as fungi and bacteria. the presence of bacteria in the midgut of sand flies can influence ... | 2017 | 29284535 |
| detection of leishmania dna and blood meal identification in sand flies (diptera: psychodidae) from lençois maranhenses national park region, brazil. | to elucidate portions of the transmission cycles of american tegumentary leishmaniasis (tl) and visceral leishmaniasis (vl) occurring in the region surrounding the lençóis maranhenses national park, an important tourist center in brazil, the present study objectives were to determine the rate of natural infection by leishmania spp. and the blood meal in caught sand flies species in the region. sand flies were captured over 36 mo in 62 locations of the municipality of barreirinhas, maranhão with ... | 2017 | 29281057 |
| the effect of luminous intensity on the attraction of phlebotomine sand flies to light traps. | to improve the efficiency of light traps in collecting phlebotomine sand flies, the potential effects of luminous intensity on the attraction of these insects to traps were evaluated. sand flies were collected with hooper pugedo (hp) light traps fitted with 5-mm light-emitting diodes (led) bulbs: green (520 nm wavelength-10,000, 15,000 and 20,000 millicandela (mcd) and blue (470 nm-4,000, 12,000 and 15,000 mcd). a total of 3,264 sand flies comprising 13 species were collected. the collected spec ... | 2017 | 29272438 |
| a defined subunit vaccine that protects against vector-borne visceral leishmaniasis. | vaccine development for vector-borne pathogens may be accelerated through the use of relevant challenge models, as has been the case for malaria. because of the demonstrated biological importance of vector-derived molecules in establishing natural infections, incorporating natural challenge models into vaccine development strategies may increase the accuracy of predicting efficacy under field conditions. until recently, however, there was no natural challenge model available for the evaluation o ... | 2017 | 29263878 |
| lipophosphoglycan polymorphisms do not affect leishmania amazonensis development in the permissive vectors lutzomyia migonei and lutzomyia longipalpis. | lipophosphoglycan (lpg) is a dominant surface molecule of leishmania promastigotes. its species-specific polymorphisms are found mainly in the sugars that branch off the conserved gal(β1,4)man(α1)-po4 backbone of repeat units. leishmania amazonensis is one of the most important species causing human cutaneous leishmaniasis in the new world. here, we describe lpg intraspecific polymorphisms in two le. amazonensis reference strains and their role during the development in three sand fly species. | 2017 | 29246180 |
| alternative splicing originates different domain structure organization of lutzomyia longipalpis chitinases. | background the insect chitinase gene family is composed by more than 10 paralogs, which can codify proteins with different domain structures. in lutzomyia longipalpis, the main vector of visceral leishmaniasis in brazil, a chitinase cdna from adult female insects was previously characterized. the predicted protein contains one catalytic domain and one chitin-binding domain (cbd). the expression of this gene coincided with the end of blood digestion indicating a putative role in peritrophic matri ... | 2018 | 29236932 |
| a temporal comparison of sex-aggregation pheromone gland content and dynamics of release in three members of the lutzomyia longipalpis (diptera: psychodidae) species complex. | lutzomyia longipalpis is the south american vector of leishmania infantum, the etiologic agent of visceral leishmaniasis (vl). male l. longipalpis produce a sex-aggregation pheromone that is critical in mating, yet very little is known about its accumulation over time or factors involved in release. this laboratory study aimed to compare accumulation of pheromone over time and determine factors that might influence release in three members of the l. longipalpis species complex. | 2017 | 29194438 |
| co-occurrence and seasonal and environmental distributions of the sandflies lutzomyia longipalpis and nyssomyia whitmani in the city of puerto iguazú, northeastern argentina. | the aim of this work was to study the distribution of phlebotominae (diptera: psycodidade) abundance in time and space in an area in northeastern argentina with vector transmission of visceral and tegumentary leishmaniasis. for this, 51 households were selected using a 'worst scenario' criterion where one light trap was set during two consecutive nights in peridomiciles in the transitions between the four seasons, and the environment was surveyed simultaneously. the relationships of phlebotomine ... | 2017 | 29178206 |
| pre-clinical antigenicity studies of an innovative multivalent vaccine for human visceral leishmaniasis. | the notion that previous infection by leishmania spp. in endemic areas leads to robust anti-leishmania immunity, supports vaccination as a potentially effective approach to prevent disease development. nevertheless, to date there is no vaccine available for human leishmaniasis. we optimized and assessed in vivo the safety and immunogenicity of an innovative vaccine candidate against human visceral leishmaniasis (vl), consisting of virus-like particles (vlp) loaded with three different recombinan ... | 2017 | 29176865 |
| clinical and immunopathological findings during long term follow-up in leishmania infantum experimentally infected dogs. | canine visceral leishmaniasis (cvl) is caused by leishmania infantum, which in the new world is transmitted by lutzomyia longipalpis. while prospective clinical and immunological assessments of dogs experimentally challenged with l. infantum have been previously reported over a relatively short follow-up period, the long-term characterization of infected animals has not been performed to date. we evaluated dogs in a subclinical state for six years following experimental infection with l. infantu ... | 2017 | 29162847 |
| human competence to transmit leishmania infantum to lutzomyia longipalpis and the influence of human immunodeficiency virus infection. | visceral leishmaniasis (vl) caused by leishmania infantum is a lethal disease transmitted by sand flies. although, considered a zoonosis with dogs held as the main reservoirs, humans are also sources of infection. therefore, control policies currently focused on dog culling may need to consider that vl and human immunodeficiency virus (hiv)/vl patients may also be infectious, contributing to transmission. reservoir competence of patients with vl without and with hiv infection and of persons asym ... | 2018 | 29141704 |
| immunization against full-length protein and peptides from the lutzomyia longipalpis sand fly salivary component maxadilan protects against leishmania major infection in a murine model. | leishmaniasis is an arthropod vectored disease causing considerable human morbidity and mortality. vaccination remains the most realistic and practical means to interrupt the growing number and diversity of sand fly vectors and reservoirs of leishmania. since transmission of leishmania is achieved exclusively by sand fly vectors via immune-modulating salivary substances, conventional vaccination requiring an unmodified host immune response for success are potentially destined to fail unless immu ... | 2017 | 29079105 |
| immunization with ljm11 salivary protein protects against infection with leishmania braziliensis in the presence of lutzomyia longipalpis saliva. | leishmania is transmitted in the presence of sand fly saliva. protective immunity generated by saliva has encouraged identification of a vector salivary-based vaccine. previous studies have shown that immunization with ljm11, a salivary protein from lutzomyia longipalpis, is able to induce a th1 immune response and protect mice against bites of leishmania major-infected lutzomyia longipalpis. here, we further investigate if immunization with ljm11 recombinant protein is able to confer cross-prot ... | 2018 | 29037520 |
| serological tests fail to discriminate dogs with visceral leishmaniasis that transmit leishmania infantum to the vector lutzomyia longipalpis. | the control of reservoirs for leishmania infantum -induced zoonotic visceral leishmaniasis requires the identification of dogs posing a population risk. here, we assessed the performance of several assays to identify lutzomyia longipalpis infectious dogs. | 2017 | 28954069 |
| environmental suitability for lutzomyia longipalpis in a subtropical city with a recently established visceral leishmaniasis transmission cycle, argentina. | visceral leishmaniasis (vl) is an endemic disease in northeastern argentina including the corrientes province, where the presence of the vector and canine cases of vl were recently confirmed in december 2008. | 2017 | 28953995 |
| dog skin parasite load, tlr-2, il-10 and tnf-α expression and infectiousness. | visceral leishmaniosis is a zoonotic disease that is transmitted by lutzomyia longipalpis sandflies. dogs are the main peri-urban reservoir of the disease, and progression of canine leishmaniosis is dependent on the type of immune response elaborated against the parasite. type 1 immunity is characterized by effective cellular response, with production of pro-inflammatory cytokines such as tumour necrosis factor alpha (tnf-α). in contrast, type 2 immunity is predominantly humoral, associated with ... | 2017 | 28929498 |
| the sand fly salivary protein lufaxin inhibits the early steps of the alternative pathway of complement by direct binding to the proconvertase c3b-b. | saliva of the blood feeding sand fly lutzomyia longipalpis was previously shown to inhibit the alternative pathway (ap) of the complement system. here, we have identified lufaxin, a protein component in saliva, as the inhibitor of the ap. lufaxin inhibited the deposition of c3b, bb, properdin, c5b, and c9b on agarose-coated plates in a dose-dependent manner. it also inhibited the activation of factor b in normal serum, but had no effect on the components of the membrane attack complex. surface p ... | 2017 | 28912782 |
| sand fly (diptera, psychodidae, phlebotominae) abundance and diversity in areas affected by the são francisco river transposition project in ceará state, brazil. | entomological surveillance of sand fly vectors was carried out to support leishmaniasis prevention and control measures in areas affected by the são francisco river transposition project. | 2017 | 28851424 |
| evaluation of chemical spraying and environmental management efficacy in areas with minor previous application of integrated control actions for visceral leishmaniasis in brazil. | leishmaniases are vector-borne diseases that are transmitted to humans through the bite of leishmania-infected phlebotomine sand flies (diptera:psychodidae). the main proved vector of visceral leishmaniais (vl) in the new world - lutzomyia longipalpis - is well-adapted to urban areas and has extensive distribution within the five geographical regions of brazil. integrated public health actions directed for the vector, domestic reservoir and humans for the control of vl are preferentially applied ... | 2017 | 28760482 |
| influences of climate change on the potential distribution of lutzomyia longipalpis sensu lato (psychodidae: phlebotominae). | this study explores the present day distribution of lutzomyia longipalpis in relation to climate, and transfers the knowledge gained to likely future climatic conditions to predict changes in the species' potential distribution. we used ecological niche models calibrated based on occurrences of the species complex from across its known geographic range. anticipated distributional changes varied by region, from stability to expansion or decline. overall, models indicated no significant north-sout ... | 2017 | 28668326 |
| the current status of the lutzomyia longipalpis (diptera: psychodidae: phlebotominae) species complex. | lutzomyia longipalpis s.l. is a complex of sibling species and is the principal vector of american visceral leishmaniasis. the present review summarises the diversity of efforts that have been undertaken to elucidate the number of unnamed species in this species complex and the phylogenetic relationships among them. a wide variety of evidence, including chemical, behavioral and molecular traits, suggests very recent speciation events and complex population structure in this group. although signi ... | 2017 | 28225906 |
| leg loss in lutzomyia longipalpis (diptera: psychodidae) due to pyrethroid exposure: toxic effect or defense by autotomy? | phlebotomine sandflies lose their legs after exposure to pyrethroids. in some insects leg loss helps to defend them from intoxication and predation, a phenomenon known as autotomy. a field observation has shown that sandflies that have lost some legs are still able to blood-feed. the aims of the study were to determine whether leg loss in sandflies, after exposure to deltamethrin, is due to autotomy and to establish the effect of the leg loss on blood-feeding. | 2017 | 28035108 |
| argentinian phlebotomine fauna, new records of phlebotominae (diptera: psychodidae) for the country and the province of chaco. | sand flies are insects of medical and veterinary importance, because some species are able to transmit several pathogens such as bartonella spp., phlebovirus spp., and protozoan parasites of the genus leishmania (ross). they are widely distributed in the americas, with recordings ranging from canada to argentina. approximately 500 phlebotominae species are known in the americas, of which it is considered that at least 56 are involved in the transmission of leishmaniasis (maroli et al. 2012). pre ... | 2016 | 27470814 |
| occurrence of lutzomyia longipalpis andhuman and canine cases of visceral leishmaniasis and evaluation of their expansion in the northwest region of the state of são paulo, brazil. | introduction this paper aims to describe the dispersion of lutzomyia longipalpis and the autochthonous occurrence of visceral leishmaniasis (vl) in the northwest region of the state of são paulo between 2007 and 2013 and to analyze their expansion. methods information about the vector and associated cases was described using maps. the incidence, mortality, and lethality of human visceral leishmaniasis (hvl) were calculated. in municipalities in which more than one hvl case occurred, incidences w ... | 2016 | 27163563 |
| first report of the main vector of visceral leishmaniasis in america, lutzomyia longipalpis (lutz, neiva, 1912) (diptera: psychodidae: phlebotominae), in southern minas gerais state, brazil. | | 2015 | 26611979 |
| performance of light-emitting diode traps for collecting sand flies in entomological surveys in argentina. | the performance of two light-emitting diode traps with white and black light for capturing phlebotomine sand flies, developed by the argentinean leishmaniasis research network (redila-wl and redila-bl traps), were compared with the traditional cdc incandescent light trap. entomological data were obtained from six sand fly surveys conducted in argentina in different environments. data analyses were conducted for the presence and the abundance of lutzomyia longipalpis, migonemyia migonei, and nyss ... | 2015 | 26611973 |
| [discriminating concentrations for three insecticides used in public health in a lutzomyia longipalpis experimental strain from colombia]. | in colombia, periurban populations of lutzomyia longipalpis , vector of the causative agent of visceral leishmaniasis in the upper and middle valley of the magdalena river, may be exposed to insecticide applications with different purposes. thus, it is important to begin a susceptibility surveillance of this species to insecticides. | 2014 | 25504252 |
| effects of azadirachtin on the biology of lutzomyia longipalpis (diptera: psychodidae: phlebotominae) adult female, the main vector of american visceral leishmaniasis. | the effects of azadirachtin a added to the sucrose diet of the adult females on the mortality, oviposition, and hatching of the sand fly vector of american visceral leishmaniasis lutzomyia longipalpis (lutz & neiva, 1912) were investigated. concentrations of 0.1, 1.0, and 10.0 microg/mg of azadirachtin significantly increased insect mortality in comparison with control insects. the same dose also significantly reduced oviposition but not hatching. after a long development period, significantly f ... | 2014 | 25118426 |
| the characterization of the fat bodies and oenocytes in the adult females of the sand fly vectors lutzomyia longipalpis and phlebotomus papatasi. | the fat body (fb) is responsible for the storage and synthesis of the majority of proteins and metabolites secreted into the hemolymph. oenocytes are responsible for lipid processing and detoxification. the fb is distributed throughout the insect body cavity and organized as peripheral and perivisceral portions in the abdomen, with trophocytes and oenocytes attached to the peripheral portion. here, we investigated the morphology and the subcellular changes in the peripheral and perivisceral fbs ... | 2014 | 24863740 |
| orientation of colonized sand flies phlebotomus papatasi, p. duboscqi, and lutzomyia longipalpis (diptera: psychodidae) to diverse honeys using a 3-chamber in-line olfactometer. | a 3-chamber in-line olfactometer designed for use with sand flies is described and tested as a high-throughput method to screen honeys for attractiveness to phlebotomus papatasi (four geographic isolates), p. duboscqi (two geographic isolates), and lutzomyia longipalpis maintained in colonies at the walter reed army institute of research. a diversity of unifloral honey odors were evaluated as a proxy for the natural floral odors that sand flies may use in orientation to floral sugar sources in t ... | 2014 | 24820561 |
| epidemiological aspects of visceral leishmaniasis in jaciara, mato grosso, brazil, 2003 to 2012. | visceral leishmaniasis (vl) is caused by leishmania (l.) infantum chagasi in the americas. in brazil, it is transmitted by sandflies of the species lutzomyia longipalpis and l. cruzi, and dogs are the main domestic reservoirs. the aim of this study was to analyze data relating to vl transmission in jaciara, state of mato grosso, and discuss vector distribution, domestic reservoirs, and human cases that occurred between 2003 and 2012. the data for analysis were obtained from the notifiable diseas ... | 2014 | 24728362 |
| [expansion of the distribution of lutzomyia longipalpis (lutz & neiva, 1912) (diptera: psychodidae) in the department of caldas: increased risk of visceral leishmaniasis]. | diverse taxonomical and molecular studies suggest that lutzomyia longipalpis , main vector of visceral leishmaniasis in latin america, is a complex of species widely distributed throughout the continent and adapted to different habitats. | 2014 | 24652141 |
| climatic factors and population density of lutzomyia longipalpis (lutz & neiva, 1912) in an urban endemic area of visceral leishmaniasis in midwest brazil. | the life cycle of vectors and the reservoirs that participate in the chain of infectious diseases have a strong relationship with the environmental dynamics of the ecosystems in which they live. oscillations in population abundance and seasonality of insects can be explained by factors inherent in each region and time period. therefore, knowledge of the relationship and influence of environmental factors on the population of lutzomyia longipalpis is necessary because of the high incidence of vis ... | 2013 | 24581349 |
| spatial dynamics of urban populations of lutzomyia longipalpis (diptera: psychodidae) in caxias, state of maranhão, brazil. | in this paper, we report the ecology of lutzomyia longipalpis in caxias city, located in the eastern part of state of maranhão, brazil and highlight its seasonal and geographical distribution by environment. in addition, we discuss natural leishmania infection and its relationship with visceral leishmaniasis. | 2013 | 24270246 |
| the dispersion of lutzomyia longipalpis in urban areas. | | 2013 | 23856862 |
| anomalies in the sand fly lutzomyia longipalpis (diptera: psychodidae) in brazil. | lutzomyia longipalpis is the main species involved in the epidemiological cycle of visceral leishmaniasis in brazil. this species shows a wide geographic distribution and belongs to a group that has 2 setae in the paramere with the curved tips towards the apex of this structure among other characteristics. morphological characters in the genitalia of phlebotomines are essential for correct identification of species. the aim of this study was to report the finding of unilateral and bilateral anom ... | 2013 | 23687856 |
| [visceral leishmaniasis in argentina. cases notification and distribution of vectors (2006-2012)]. | visceral leishmaniasis (vl) is a notifiable disease in argentina. every new case diagnosed must be reported to the national health surveillance system. in the period 2006-2012, a total of 103 cases have been notified in four provinces: entre rios, santa fe, misiones and santiago del estero. misiones has the largest amount of cases: a total of 80, detected in 15 towns from may 2006 to july 2012. the highest incidence is observed in children (less than 15 years of age). in 2004 the vector lutzomyi ... | 2013 | 23570757 |
| survey of sandfly fauna (diptera: psychodidae) in uberlândia, minas gerais state, brazil, 2003-2004. | we analyzed the sandflies around houses and domestic animal shelters located in residences close to forests in localities on the banks of the araguari river, uberlândia, mg, from february 2003 to november 2004. the phlebotomines were captured in the peridomiciliary area, where shannon traps were utilized in the peridomicile and cdc traps in animal shelters. 2,783 specimens of sandflies were captured, 2,140 females (76.9%) and 643 males (23.1%), distributed between 17 species. the most abundant s ... | 2013 | 23563760 |
| ecology of phlebotomines (diptera, psychodidae) in rural foci of leishmaniasis in tropical brazil. | this work aimed to study the community structure of sandflies, with regard to the richness, constancy, abundance, and monthly frequency of the species with a focus on the transmission of leishmaniasis. | 2012 | 23295871 |
| sandflies (diptera: psychodidae) from caves of the quartzite espinhaço range, minas gerais, brazil. | this study investigated the sandfly fauna of two quartzite caves in the espinhaço mountain range, located in the municipality of diamantina, state minas gerais. from august 2010-july 2011, entomological sampling was performed in the caves of salitre and monte cristo with two hp light traps exposed in the photic and aphotic zones of each cave. the sandfly fauna consisted of 17 species, among which lutzomyia cipoensis was predominant (54.76%). the male/female ratio in the total captures was 1:2.5. ... | 2012 | 23295752 |
| the first record of lutzomyia longipalpis (lutz & neiva, 1912) (diptera: psychodidae: phlebotominae) in the state of paraná, brazil. | we report the first find of lutzomyia longipalpis in the state of paraná, brazil. | 2012 | 23152351 |
| profile and geographic distribution of reported cases of visceral leishmaniasis in campo grande, state of mato grosso do sul, brazil, from 2002 to 2009. | this study sought to describe the profile and geographic distribution of reported cases of visceral leishmaniasis (vl) in the city of campo grande, state of mato grosso do sul (ms), brazil, from 2002 to 2009. | 2012 | 23152344 |
| relationship between digestive enzymes and food habit of lutzomyia longipalpis (diptera: psychodidae) larvae: characterization of carbohydrases and digestion of microorganisms. | the sandfly lutzomyia longipalpis (lutz and neiva, 1912) is the main vector of american visceral leishmaniasis. in spite of its medical importance and several studies concerning adult digestive physiology, biochemistry and molecular biology, very few studies have been carried out to elucidate the digestion in sandfly larvae. even the breeding sites and food sources of these animals in the field are largely uncharacterized. in this paper, we describe and characterize several carbohydrases from th ... | 2012 | 22684112 |
| disruption of the peritrophic matrix by exogenous chitinase feeding reduces fecundity in lutzomyia longipalpis females. | lutzomyia longipalpis is the most important vector of visceral leishmaniasis in brazil. when female sandflies feed on blood, a peritrophic matrix (pm) is formed around the blood bolus. the pm is secreted by midgut cells and composed of proteins, glycoproteins and chitin microfibrils. the pm functions as both a physical barrier against pathogens present in the food bolus and blood meal digestion regulator. previous studies of mosquitoes and sandflies have shown that the absence of a pm, resulting ... | 2012 | 22666867 |
| biotic factors and occurrence of lutzomyia longipalpis in endemic area of visceral leishmaniasis, mato grosso do sul, brazil. | the relationships between environmental exposure to risk agents and health conditions have been studied with the aid of remote sensing imagery, a tool particularly useful in the study of vegetation cover. this study aims to evaluate the influence of environmental variables on the spatial distribution of the abundance of lutzomyia longipalpis and the reported canine and human visceral leishmaniasis (vl) cases at an urban area of campo grande, state of mato grosso do sul. the sandfly captures were ... | 2012 | 22510836 |
| report of lutzomyia longipalpis (lutz & neiva, 1912) (diptera: psychodidae: phlebotominae) in a cutaneous-leishmaniasis-endemic area of panama. | lutzomyia longipalpis is the primary vector of the parasite responsible for visceral leishmaniasis in the americas. in the present study, lu. longipalpis was found in a domiciliary area in limón, a district in capira, a region in which cutaneous leishmaniasis is endemic in panama. previously, this species has been found in a humid forest in this same region. finding lu. longipalpis in domiciliary areas indicates that this species may be adapting to new habitats and that it may play a role in the ... | 2011 | 22241132 |
| assessment of sand fly (diptera, psychodidae) control using cypermethrin in an endemic area for visceral leishmaniasis, montes claros, minas gerais state, brazil. | montes claros in minas gerais state, brazil, was considered an intense transmission area for visceral leishmaniasis. this study evaluated sand fly fauna after insecticide application. captures were performed in 10 districts from september 2005 to august 2006 with cdc light traps inside and outside each residence. cypermethrin was sprayed in two cycles during november/2005 and may/2006. the 636 specimens collected, belonging to 10 species, were predominantly lutzomyia longipalpis (79%), and most ... | 2011 | 22124489 |
| possible implication of the genetic composition of the lutzomyia longipalpis (diptera: psychodidae) populations in the epidemiology of the visceral leishmaniasis. | lutzomyia longipalpis (diptera: psychodidae) is the principal vector of american visceral leishmaniasis. several studies have indicated that the lu. longipalpis population structure is complex. it has been suggested that genetic divergence caused by genetic drift, selection, or both may affect the vectorial capacity of lu. longipalpis. however, it remains unclear whether genetic differences among lu. longipalpis populations are directly implicated in the transmission features of visceral leishma ... | 2011 | 21936320 |
| pheromone gland development and pheromone production in lutzomyia longipalpis (diptera: psychodidae: phlebotominae). | the sand fly lutzomyia longipalpis (lutz & neiva) (diptera: psychodidae: phlebotominae) is the main vector of american visceral leishmaniasis. adult males produce a terpenoid sex pheromone that in some cases also acts as male aggregation pheromone. we have analyzed the correlation between male pheromone production levels and pheromone gland cell morphogenesis after adult emergence from pupae. the abdominal tergites of l. longipalpis males were dissected and fixed in glutaraldehyde for transmissi ... | 2011 | 21661306 |
| copulatory courtship song in lutzomyia migonei (diptera: psychodidae). | lutzomyia migonei is a vector of leishmaniasis with a wide distribution in south america, which could favour population differentiation and speciation. cryptic species of the lutzomyia longipalpis complex, the widely distributed sand fly vector of visceral leishmaniasis in latin america, have previously been shown to display distinct copulation songs. we found that lu. migonei males also produce a song during copulation. this "lovesong" presents short trains (6-8 pulses) with an inter-pulse inte ... | 2010 | 21225208 |
| monitoring of lutzomyia longipalpis lutz & neiva, 1912 in an area of intense transmission of visceral leishmaniasis in rio grande do norte, northeast brazil. | urban increase of visceral leishmaniasis (vl) in brazil is associated with the adaptation of its vector, lutzomyia longipalpis, to environments modified by humans. the present study reports the results of an entomological monitoring of l. longipalpis and the effect of environmental variables on its population density. sandflies were captured in the municipality of mossoró, state of rio grande do norte, northeastern brazil, from january 2005 to december 2006. two cdc light traps were placed month ... | 2010 | 20385058 |
| should reproductively isolated populations of lutzomyia longipalpis sensu lato receive taxonomically valid names? | a group of 18 research workers involved in different aspects of the biology of lutzomyia longipalpis discussed whether or not it is important to give taxonomically valid names to populations that have been defined by biological, biochemical and molecular methods to be reproductively isolated. the type material of this medically important species has been lost and because of this it was recommended that a colony should be established from insects captured in the region of the type area and that t ... | 2009 | 20140386 |
| salivary gland homogenates of lutzomyia longipalpis and its vasodilatory peptide maxadilan cause plasma leakage via pac1 receptor activation. | experiments were designed to determine if salivary gland homogenates (sgh) of the sand fly lutzomyia longipalpis, the vasodilatory peptides maxadilan and pituitary adenylate cyclase-activating peptide (pacap-38) may cause plasma leakage and to what extent these effects could be due to pac1 receptor stimulation. | 2009 | 19176972 |
| fine structure and distribution pattern of antennal sensilla of lutzomyia longipalpis (diptera: psychodidae) sand flies. | the specific aims of this work were to examine the antennal sensilla of lutzomyia longipalpis (lutz & neiva 1912) (diptera: psychodidae) adults and to characterize their typology and topography, with special attention to olfactory sensilla. the surfaces of the antennal segments of lu. longipalpis males and females were studied by scanning electron microscopy (sem). lu. longipalpis used in the current study were obtained from a colony originating from lapinha cave, minas gerais state, brazil. mic ... | 2008 | 19058620 |
| genetic divergence between two sympatric species of the lutzomyia longipalpis complex in the paralytic gene, a locus associated with insecticide resistance and lovesong production. | the sandfly lutzomyia longipalpis s.l. is the main vector of american visceral leishmaniasis. l. longipalpis s.l. is a species complex but until recently the existence of cryptic sibling species among brazilian populations was a controversial issue. a fragment of paralytic (para), a voltage dependent sodium channel gene associated with insecticide resistance and courtship song production in drosophila, was isolated and used as a molecular marker to study the divergence between two sympatric sibl ... | 2008 | 19057828 |
| changes in amounts of total salivary gland proteins of lutzomyia longipallpis (diptera: psychodidae) according to age and diet. | saliva plays important roles in facilitation of a bloodmeal, lubrication of mouthparts, and parasite transmission for some vector insects. salivary composition changes during the lifetime of an insect, and differences in the salivary profile may influence its functions. in this report, the amount and profile of salivary gland protein of the american visceral leishmaniasis vector lutzomyia longipalpis (lutz & neiva, 1912) were analyzed at different times of insect development and diet. protein co ... | 2008 | 18533433 |
| courtship behaviour in the sandfly lutzomyia longipalpis, the new world vector of visceral leishmaniasis. | courtship behaviour in lutzomyia longipalpis lutz and neiva (diptera: psychodidae) was examined to determine the sequence of behaviours that occur prior to copulation. courtship consisted of a series of male and female touching and wing-flapping behaviours, with males performing a greater variety of wing-flapping behaviours than previously described. occurrence of male approach-flapping, semi-circling and female stationary-flapping were all predictors of eventual copulation, and may coincide wit ... | 2007 | 18092971 |
| effects of azadirachtin on the development and mortality of lutzomyia longipalpis larvae (diptera: psychodidae: phlebotominae). | the effects of azadirachtin a added to the standard diet on the development, mortality, and metamorphosis of lutzomyia longipalpis lutz & neiva, 1912 were studied. concentrations of 0.1, 1.0, and 10.0 microg of azadirachtin/mg of diet significantly increased larval mortality in comparison with nontreated insects. concentrations 0.1 and 1.0 microg blocked the molt of larvae, which remained as third instars until the end of the experiment. the 10 microg/mg concentration resulted in greater molt in ... | 2006 | 16619609 |
| structure and distribution of sensilla on maxillary palps and labella of lutzomyia longipalpis (diptera: psychodidae) sand flies. | the typology, number, size, and distribution pattern of labellar and palpal sensilla of male and female lutzomyia longipalpis (lutz and neiva, 1912) sand flies were analyzed by scanning electron and light microscopy, contributing to the construction of a gustatory and olfactory map in this important vector species. about 35 trichoid sensilla, sorted into three different classes according to their lengths, are present on the two labellar lobes. five distinct structures are observed in the maxilla ... | 2005 | 16003785 |
| mitochondrial cytochrome b variation in populations of the visceral leishmaniasis vector lutzomyia longipalpis across eastern brazil. | a population analysis of peridomestic, light-trapped, field specimens of the phlebotomine sand fly lutzomyia longipalpis was targeted to six locations representing a geographic transect across eastern brazil. mitochondrial cytochrome b gene sequences established the pattern of genetic variation among the populations. alignment of a 261-basepair region at the 3' end of cytochrome b identified 30 haplotypes and 21 segregating sites from 78 sand flies. pairwise comparisons indicated statistically s ... | 2003 | 14640498 |
| the taxonomic status of genetically divergent populations of lutzomyia longipalpis (diptera: psychodidae) based on the distribution of mitochondrial and isozyme variation. | the sand fly, lutzomyia longipalpis (lutz & neiva) reputedly is a complex of cryptic species; however, there is currently no consensus as to the number of species in the complex or their geographic distributions. we conducted phylogenetic analyses of 31 populations from throughout the species range, using seven isozyme loci and genes in the mitochondrial genome. analyses of these two independent sets of markers were largely concordant and revealed four distinct clades that support the existence ... | 2003 | 14596274 |
| navigation of lutzomyia longipalpis (diptera: psychodidae) under dusk or starlight conditions. | the responses of male and female lutzomyia longipalpis (lutz & neiva) to different wavelengths of light was tested by presenting the sandflies with two light sources simultaneously, a series of test wavelengths between 350-670 nm and a 400 nm control. to test whether l. longipalpis could discriminate between the test and control, three sets of experiments were carried out in which the test wavelengths were presented at higher, equivalent or lower intensity than the control. in all three experime ... | 2003 | 12908917 |
| [approximation to the cdc method to determine insecticide susceptibility in leishmaniasis vectors]. | the centers for disease control (cdc, usa) has proposed a simplified method for the determinations of insecticide resistance in adult mosquitoes, using 250 ml wheaton bottles containing measured dosages. insects are transferred into the bottle for 1 hour and monitored for mortality at regular intervals. in standardizing the cdc method for use with phlebotomine sand flies, effects of the solvent without insecticide were evaluated. two colonized sand fly vector species were used: lutzomyia longipa ... | 2003 | 12696404 |
| preliminary assays indicate that antonia ovata (loganiaceae) and derris amazonica (papilionaceae), ichthyotoxic plants used for fishing in roraima, brazil, have an insecticide effect on lutzomyia longipalpis (diptera: psychodidae: phlebotominae). | laboratory-reared lutzomyia longipalpis (lutz and neiva 1912) was tested with extracts of two ichthyotoxic plants, known as timbós, used as fishing poison in the amazon. phlebotomines, l. longipalpis, and plants, antonia ovata and derris amazonica, were collected in the raposa-serra do sol indian reserve, a focus of visceral leishmaniasis in the state of roraima, brazil. extracts were prepared from dried leaves of a. ovata and roots of d. amazonica that were percolated in water, filtered and dri ... | 2002 | 12219144 |
| [standardization of the method proposed by the world health organization for determining the susceptibility levels of leishmaniasis vectors to insecticides]. | the who method for determining insecticide resistance was standardized for several species of lutzomyia sand flies under laboratory and field conditions. the biological assays were applied solely to optimize the conditions for the control, i.e., without insecticide, and to estimate mortality due to handling or other unfavorable conditions. adult female flies from 3 laboratory colonies and one field strain were tested: two laboratory strains of lutzomyia longipalpis, one laboratory strain of lutz ... | 2002 | 12152485 |
| copulation songs in three siblings of lutzomyia longipalpis (diptera: psychodidae). | we present the results of recording male courtship songs of the sandfly lutzomyia longipalpis. the striking differences in the songs from 3 brazilian populations of this sandfly with 3 distinct male pheromones support the 3 sibling species previously proposed based on this characteristic. | 2006 | 11925981 |
| external morphology of sensory structures of fourth instar larvae of neotropical species of phlebotomine sand flies (diptera: psychodidae) under scanning electron microscopy. | in the present study, some morphological structures of antennae, maxillary palps and caudal setae of fourth instar larvae of laboratory-reared phlebotomine sand flies (lutzomyia longipalpis, l. migonei, l. evandroi, l. lenti, l. sericea, l. whitmani and l. intermedia) of the state of ceará, brazil, were examined under scanning electron microscopy. the antennal structures exhibited considerable variation in the morphology and position. a prominent digitiform distal segment has been observed only ... | 2001 | 11784930 |
| ultrastructure of endocrine cells from the abdominal midgut epithelium of lutzomyia longipalpis (diptera: psychodidae). | transmission electron microscopy (tem) was used to study two types of endocrine cells front the midgut of adult female luttzomyia longipalpis (lutz & neiva). endocrine cells rarely have been observed in nematocera, even using tem, and were present in small numbers dispersed among the monolayer of midgut digestive epithelial cells. triangular shaped "closed" cells were observed along the basement membrane, bounded on each side by digestive cells; these cells closed distally before reaching the ep ... | 2001 | 11580051 |
| new molecular markers for phlebotomine sand flies. | using degenerate-primers pcr we isolated and sequenced fragments from the sand fly lutzomyia longipalpis homologous to two behavioural genes in drosophila, cacophony and period. in addition we identified a number of other gene fragments that show homology to genes previously cloned in drosophila. a codon usage table for l. longipalpis based on these and other genes was calculated. these new molecular markers will be useful in population genetics and evolutionary studies in phlebotomine sand flie ... | 2001 | 11334955 |
| courtship song genes and speciation in sand flies. | lutzomyia longipalpis (lutz & neiva, 1912) (diptera: psychodidae: phlebotominae) is a vector of visceral leishmaniasis in the americas and it might represent a complex of sibling species. reproductive isolation between closely related species often involves differences in courtship behaviour. cacophony (cac) and period (per) are two drosophila genes that control features of the "lovesong" males produce during courtship that has been implicated in the sexual isolation between closely related spec ... | 2001 | 11313653 |
| speciation and population structure in the morphospecies lutzomyia longipalpis (lutz & neiva) as derived from the mitochondrial nd4 gene. | recent studies have suggested that the phlebotomine sand fly lutzomyia longipalpis (diptera: psychodidae), the principal vector of visceral leishmaniasis in the neotropics, may consist of several allopatric sibling species. phylogenetic and population genetic analyses of nucleotide variation in a 618-bp fragment of the mitochondrial nd4 gene were carried out on specimens of lu. longipalpis from several locations in central and south america. the analyses were concordant with previous findings, i ... | 2001 | 11161745 |
| sensilla on the terminalia of lutzomyia spp. (diptera: psychodidae) sand flies. | sensilla on the male terminalia of four different species of sand flies (diptera: psychodidae) are described. two species belonged to the same species complex: lutzomyia longipalpis (lutz & neiva) and l. cruzi (mangabeira). other brazilian species complex was represented by l. lenti (mangabeira) and l. carmelinoi (ryan, fraiha, lainson & shaw). approximately 5-15 sensilla were found on the initial part of the styles of all species. structurally, the sensory organs were similar to small coeloconi ... | 2000 | 11126541 |
| posterior spiracles of fourth instar larvae of four species of phlebotomine sand flies (diptera: psychodidae) under scanning electron microscopy. | in the present study, posterior spiracles of laboratory-reared fourth instar larvae of lutzomyia longipalpis, l. migonei, l. lenti, and l. whitmani (diptera: psychodidae) of the state of ceará, brazil, were examined under scanning electron microscopy. the number of papillae of spiracles examined varied according to the species examined, but no intraspecific differences were found. the importance of this structure to sand fly larva identification and phylogeny is commented. | 2003 | 10998217 |
| "the salivary 5'-nucleotidase/phosphodiesterase of the hematophagus sand fly, lutzomyia longipalpis" by jose m.c. ribeiro, edgar d. rowton and rosane charlab. insect biochemistry and molecular biology 30(4) pp.279-285 | | 2000 | 10989299 |
| the salivary adenosine deaminase from the sand fly lutzomyia longipalpis. | in the process of sequencing a subtracted cdna library from the salivary glands of the sand fly lutzomyia longipalpis, we identified a cdna with similarities to gene products of the adenosine deaminase family. prompted by this cdna finding, we detected adenosine deaminase activity at levels of 1 u/mg protein in salivary gland homogenates. the activity was significantly reduced following a blood meal indicating its apparent secretory fate. the native enzyme has a k(m) of approximately 10 microm, ... | 2000 | 10864517 |
| aspects related to productivity for four generations of a lutzomyia longipalpis laboratory colony. | a closed colony of lutzomyia longipalpis was established with specimens collected in the raposa - serra do sol indian reservoir, one of the main foci of visceral leishmaniasis in the state of roraima, brazil. biological observations were made on four generations of a l. longipalpis colony with emphasis on productivity. aspects studied were the number of laid and retained eggs, and the number of adults (male and female) per generation. during the four generations the percentage of engorged female ... | 2006 | 10733749 |
| the salivary 5'-nucleotidase/phosphodiesterase of the hematophagus sand fly, lutzomyia longipalpis [corrected]. | salivary gland homogenates from adult female lutzomyia longipalpis sand flies contain large amounts of 5'-nucleotidase and phosphodiesterase activities. phosphodiesterase activity was found to be associated with 5'-nucleotidase in several independent experiments: (i) it coelutes with 5'-nucleotidase on a molecular sieving column, (ii) it coelutes with 5'-nucleotidase on a chromatofocusing column, and (iii) it has the same thermal inactivation kinetics as the 5'-nucleotidase activity. additionall ... | 2000 | 10727894 |
| metaphase karyotypes and g-banding in sandflies of the lutzomyia longipalpis complex. | mitotic metaphase chromosomes (2n = 8) from brain cells of fourth instar sandfly larvae of four geographical strains of the lutzomyia longipaplis complex were examined microscopically, with bright-field illumination, after staining by a new g-banding technique involving exposure of air-dried chromosome preparations to quinacrine and ultraviolet light. differences of g-banding and/or position of the centromere on chromosome 4 (the smallest chromosome pair) distinguished four putative sibling spec ... | 1999 | 10194752 |