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baseline insecticide susceptibility screening against six active ingredients for culex and aedes (diptera: culicidae) mosquitoes in the united states.mosquitoes may develop resistance to insecticide active ingredients (ai). thus, mosquitoes should be tested for resistance to confirm efficacy of insecticide-based control, inform management decisions, and protect public and environmental health. our objectives were to determine a baseline of resistance for six ais used in mosquito control in the united states to assess how resistance differs between mosquito collection location, ai, and mosquito species (container-ovipositing aedes and culex th ...201728399272
pirna-3312: a putative role for pyrethroid resistance in culex pipiens pallens (diptera: culicidae).piwi-interacting rnas (pirnas) are a newly identified class of small noncoding rnas. they are associated with chromatin organization, messenger rna stability, and genome structure. although the overexpression of pirna-3312 in deltamethrin-susceptible (ds) strain of culex pipiens pallens (l.) was observed in our previous large-scale transcriptome data, the roles of pirna in insecticide resistance have not been clearly defined. the aim of the present study was to investigate how pirna-3312 is invo ...201728399266
life table analysis of culex pipiens under simulated weather conditions in egypt.the effect of fluctuating ambient air and water temperatures and relative humidity (rh) on the population parameters of culex pipiens was studied using life table data during the winter-spring and summer-autumn seasons in giza province, egypt. in winter-spring (20.46 ± 1.8°c and 51.68 ± 3.63% rh), the preadult survival rate for cx. pipiens was 80.65%, which was higher than the 50.00% observed in the summer-autumn (30.19 ± 0.67°c and 52.68 ± 8.04% rh). during the winter-spring period, the develop ...201728388321
mosquito allergy in children: clinical features and limitation of commercially-available diagnostic tests.to determine the clinical features of mosquito allergy in children and the ability of commercially available mosquito allergy tests to detect children with mosquito allergy in thailand.201728364407
blood-feeding patterns of native mosquitoes and insights into their potential role as pathogen vectors in the thames estuary region of the united kingdom.the range of vertebrate hosts on which species of mosquito blood-feed is an important parameter for identifying potential vectors and in assessing the risk of incursion and establishment of vector-borne pathogens. in the united kingdom, studies of mosquito host range have collected relatively few specimens and used techniques that could only broadly identify host species. this study conducted intensive collection and analysis of mosquitoes from a grazing marsh environment in southeast england. t ...201728347323
the role of mir-2∼13∼71 cluster in resistance to deltamethrin in culex pipiens pallens.excessive and continuous application of deltamethrin has resulted in the development of deltamethrin resistance among mosquitoes, which becomes a major obstacle for mosquito control. in a previous study, differentially expressed mirnas between deltamethrin-susceptible (ds) strain and deltamethrin-resistant (dr) strain using illumina sequencing in culex pipiens pallens were identified. in this study, we applied rnai and the centers for disease control and prevention (cdc) bottle bioassay to inves ...201728342977
co-circulation of two usutu virus strains in northern italy between 2009 and 2014.usutu virus is an arbovirus closely related to west nile virus (genus flavivirus), which circulates between mosquitoes and wild birds. this virus has been increasingly reported in europe, raising concerns for its possible pathogenic potential for wild birds and humans. this study reports the whole genome sequences of 15 strains of usuv, isolated between 2010 and 2014 from mosquitoes and wild birds in the course of west nile virus surveillance in the emilia-romagna and lombardy regions of norther ...201728341546
complete genomic and ultrastructural analysis of a nam dinh virus isolated from culex pipiens quinquefasciatus in china.the nam dinh virus (ndiv) was isolated from culex quinquefasciatus in shenzhen, china, for the first time, in 2011. in this study, we characterized the ultrastructure of ndiv, determined its complete genome sequence and made comparisons with other known nidoviruses. electron microscopic observation revealed that the ndiv strain isolated in china produced viral nucleocapsid-like particles and vesicles in host cells. the extracellular virions were enveloped and were spherical with short spikes. th ...201728325899
co-circulation of west nile virus and distinct insect-specific flaviviruses in turkey.active vector surveillance provides an efficient tool for monitoring the presence or spread of emerging or re-emerging vector-borne viruses. this study was undertaken to investigate the circulation of flaviviruses. mosquitoes were collected from 58 locations in 10 provinces across the aegean, thrace and mediterranean anatolian regions of turkey in 2014 and 2015. following morphological identification, mosquitoes were pooled and screened by nested and real-time pcr assays. detected viruses were f ...201728320443
 the intertidal midge clunio marinus is distributed along the european coast-line. stocks from distinct locations differ in the programming of their emergence time. they represent ecological races. the crossability between 7 stocks was examined.between some stocks a non-reciprocal cross sterility has been found, characterized by fertile hybrids in one cross direction, and no offspring in the reciprocal crosses.the cross jean ♀xsan ♂ was fertile, while the cross san ♀xjean ♂ was sterile. after thr ...197128312441
[organisation, capacity for regeneration and differentiation of leg primordia of culex pipiens (l.)].1. discs of the foreleg ofculex pipiens transplanted from the fourth instar of larvae into larval hosts of the same age differentiate normal adult organs. 2. experiments in fragmentation indicate the position of the primordia of individual parts of the leg in the disc. in addition to this certain structural elements can be located within parts of the leg. 3. among the majority of host larvae the operation results in a prolongation of the fourth larval instar. in whole discs as well as in disc fr ...196928304490
toxicity of β-citronellol, geraniol and linalool from pelargonium roseum essential oil against the west nile and filariasis vector culex pipiens (diptera: culicidae).insect vectors are responsible for spreading devastating parasites and pathogens. a large number of botanicals have been suggested for eco-friendly control programs against mosquito vectors, and some of them are aromatic plants. pelargonium roseum, a species belonging to the geraniaceae family, due to its pleasant rose-like odor may represent a suitable candidate as mosquito repellent and/or larvicide. in this research, we evaluated the toxicity of the essential oil from p. roseum and its major ...201728297637
a novel rhabdovirus, related to merida virus, in field-collected mosquitoes from anatolia and thrace.next-generation sequencing technologies have significantly facilitated the discovery of novel viruses, and metagenomic surveillance of arthropods has enabled exploration of the diversity of novel or known viral agents. we have identified a novel rhabdovirus that is genetically related to the recently described merida virus via next-generation sequencing in a mosquito pool from thrace. the complete viral genome contains 11,798 nucleotides with 83% genome-wide nucleotide sequence similarity to mer ...201728283817
insecticide resistance and cytochrome-p450 activation in unfed and blood-fed laboratory and field populations of culex pipiens pallens.understanding the mechanisms of insecticide resistance to vector mosquitoes is critical for the implementation of effective control measures. a nulliparous susceptible culex pipiens pallens (kscp) laboratory colony and two field strains from paju (paj) and jeonju (jeo) korea were evaluated for susceptibility to five pesticides by microapplication techniques. unfed paj and jeo females demonstrated increased resistance compared to unfed kscp females, respectively. while blood-fed kscp females demo ...201728275327
mosgctl-7, a c-type lectin protein, mediates japanese encephalitis virus infection in mosquitoes.japanese encephalitis virus (jev) is an arthropod-borne flavivirus prevalent in asia and the western pacific, and is the leading cause of viral encephalitis. jev is maintained in a transmission cycle between mosquitoes and vertebrate hosts, but the molecular mechanisms by which the mosquito vector participates in transmission are unclear. we investigated the expression of all c-type lectins during jev infection in aedes aegypti. c-type lectin mosgctl-7 (aael002524) was significantly up-regulated ...201728250133
various bioactivity and relationship of structure-activity of matrine analogues.for the first time, the botanic source natural product matrine was reported to have more potent inhibitory activity against tobacco mosaic virus (tmv) than the commercial virucide ribavirin. on the basis of the structural diversity modification strategy, a series of matrine derivatives was synthesized and systematically evaluated for their antiviral activity against tmv, fungicidal activity, and insecticidal activity. as a result, compounds 3 (inhibitory rate 67.3%, 69.5%, 63.7%, 63.0% at 500 μg ...201728248103
the effect of interspecific competition on the temporal dynamics of aedes albopictus and culex pipiens.aedes albopictus and culex pipiens larvae reared in the same breeding site compete for resources, with an asymmetrical outcome that disadvantages only the latter species. the impact of these interactions on the overall ecology of these two species has not yet been assessed in the natural environment. in the present study, the temporal patterns of adult female mosquitoes from both species were analysed in north-eastern italy, and substantial temporal shifts between abundance curves of cx. pipiens ...201728228159
cloning and expression of cry2aa from native bacillus thuringiensis strain sy49-1 and its insecticidal activity against culex pipiens (diptera: culicidae).bacillus thuringiensis (berliner) (bt) is well known for having toxicity against pest insects because of their ability to form endospores and broad-range activity of their parasporal inclusions. in this study, a new member of cry2a gene from previously characterized native b. thuringiensis sy49-1 strain was cloned, expressed and used for its activity against culex pipiens (diptera: culicidae) larvae. the sequence analysis of the cloned cry2a gene revealed that it encodes a polypeptide of 633 aa ...201728215855
inter-epidemic abundance and distribution of potential mosquito vectors for rift valley fever virus in ngorongoro district, tanzania.background rift valley fever (rvf) is a mosquito-borne viral zoonosis that primarily affects ruminants but also has the capacity to infect humans. objective to determine the abundance and distribution of mosquito vectors in relation to their potential role in the virus transmission and maintenance in disease epidemic areas of ngorongoro district in northern tanzania. methods a cross-sectional entomological investigation was carried out before the suspected rvf outbreak in october 2012. mosquitoe ...201528156815
molecular xenomonitoring (mx) and transmission assessment survey (tas) of lymphatic filariasis elimination in two villages, menoufyia governorate, egypt.lymphatic filariasis (lf) is focally endemic in egypt where the female mosquito, culex pipiens, is responsible for its transmission. the aim of the study was to investigate the impact of implementation of the 13th round of mda in two egyptian villages in the menoufyia governorate area after failing the transmission assessment survey (tas) in 2005 using two methods, and to decide whether it is safe to stop mda in these, as well as in similar implementation units (ius). to achieve this aim, both t ...201728155014
identification of larvicidal constituents of the  essential oil of echinops grijsii roots against the  three species of mosquitoes.the screening of chinese medicinal herbs for insecticidal principles showed that the essential oil of echinops grijsii hance roots possessed significant larvicidal activity against mosquitoes. the essential oil was extracted via hydrodistillation and its constituents were determined by gas chromatography-mass spectrometry (gc-ms) analysis. gc-ms analyses revealed the presence of 31 components, with 5-(3-buten-1-yn-1-yl)-2,2'-bithiophene (5-bbt, 27.63%), αterthienyl (α-t, 14.95%),1,8-cineole (5.5 ...201728134799
malaria transmission and the sensitivity of aggressive mosquitoes to insecticides in a poorly urbanized area of the deido health district in douala (cameroon).the design of an appropriate strategy for mosquito control in urban areas must take into account the local ecoclimate and its particularities. this study sought to determine the dynamics of malaria transmission and the level of insecticide susceptibility of aggressive mosquitoes in ndogbong, a poorly urbanized district of douala. mosquitoes were captured on volunteers in 4 houses and outdoors for 2 consecutive nights a month, from april to september 2014, and identified by molecular and morpholo ...201728132950
comparative analysis of the circadian rhythm genes period and timeless in culex pipiens linnaeus, 1758 (diptera, culicidae).nucleotide sequences of the circadian rhythm genes, period and timeless, were studied for the first time in mosquitoes culex pipiens linnaeus, 1758. in this work we evaluated variations of the studied genome fragments for the two forms of culex pipiens (forma "pipiens" - mosquitoes common for aboveground habitats, forma "molestus" - underground mosquitoes). we compared culex pipiens from russia with transatlantic culex pipiens and subtropical culex quinquefasciatus say, 1823. our results show th ...201628123673
experimental transmission of zika virus by mosquitoes from central europe.mosquitoes collected in germany in 2016, including culex pipiens pipiens biotype pipiens, culex torrentium and aedes albopictus, as well as culex pipiens pipiens biotype molestus (in colony since 2011) were experimentally infected with zika virus (zikv) at 18 °c or 27 °c. none of the culex taxa showed vector competence for zikv. in contrast, aedes albopictus were susceptible for zikv but only at 27 °c, with transmission rates similar to an aedes aegypti laboratory colony tested in parallel.201728106528
preliminary results on the control of aedes spp. in a remote guatemalan community vulnerable to dengue, chikungunya and zika virus: community participation and use of low-cost ecological ovillantas for mosquito control.objective: to study the effectiveness of an integrated intervention of health worker training, a low-cost ecological mosquito ovitrap, and community engagement on aedes spp. mosquito control over 10 months in 2015 in an urban remote community in guatemala at risk of dengue, chikungunya and zika virus transmission. methods: we implemented a three-component integrated intervention consisting of: web-based training of local health personnel in vector control, cluster-randomized assignment of an eco ...201628105304
global identification of micrornas associated with chlorantraniliprole resistance in diamondback moth plutella xylostella (l.).the diamondback moth (dbm), plutella xylostella (l.), is one of the most serious cruciferous pests and has developed high resistance to most insecticides, including chlorantraniliprole. previous studies have reported several protein-coding genes that involved in chlorantraniliprole resistance, but research on resistance mechanisms at the post-transcription level is still limited. in this study, a global screen of micrornas (mirnas) associated with chlorantraniliprole resistance in p. xylostella ...201728098189
commensal viruses of mosquitoes: host restriction, transmission, and interaction with arboviral pathogens.recent advances in virus detection strategies and deep sequencing technologies have enabled the identification of a multitude of new viruses that persistently infect mosquitoes but do not infect vertebrates. these are usually referred to as insect-specific viruses (isvs). these novel viruses have generated considerable interest in their modes of transmission, persistence in mosquito populations, the mechanisms that restrict their host range to mosquitoes, and their interactions with pathogens tr ...201728096646
blood meal identification of the mosquito (diptera: culicidae) specimens belong to culex pipiens complex that were collected from kayseri province.this study aimed to determine the host preferences in blood meal of specimens belonging to culex pipiens complex.201628091388
european aedes albopictus and culex pipiens are competent vectors for japanese encephalitis virus.japanese encephalitis virus (jev) is the causative agent of japanese encephalitis, the leading cause of viral encephalitis in asia. jev transmission cycle involves mosquitoes and vertebrate hosts. the detection of jev rna in a pool of culex pipiens caught in 2010 in italy raised the concern of a putative emergence of the virus in europe. we aimed to study the vector competence of european mosquito populations, such as cx. pipiens and aedes albopictus for jev genotypes 3 and 5.201728085881
an updated review on phytochemical properties of "golden dewdrop" duranta erecta.duranta erecta (family: verbenaceae) commonly referred to golden dewdrop, pigeon berry, angel whisper, or skyflower is one of the traditional medicinal plants. it has been shown to possess antimicrobial, antioxidant, and insecticide properties. its phytoconstituents such as alkaloids, flavonoids, glycosides, phenolics, saponins, steroids, tannins, and terpenoids are reported as the basis of its efficacious therapeutic properties. the other important constituents which contribute to the remedial ...028082794
comparison of adult mosquito black-light and light-emitting diode traps at three cowsheds located in malaria-endemic areas of the republic of korea.adult mosquito surveillance and field trials evaluated selected commercially available ultraviolet black-light (bl) and light-emitting diode (led) traps at three sites where vivax malaria is endemic from may to october 2015 in northwestern republic of korea. collections totaled 283,929 adult mosquitoes (280,355 [98.74%] females and 3,574 [1.26%] males) comprising 17 species (including six members of the anopheles hyrcanus group) belonging to six genera. the four most predominant female species c ...201728082650
the search for novel insecticide targets in the post-genomics era, with a specific focus on g-protein coupled receptors.insects are considered pests globally, implicated in the destruction of agricultural fields and transmission of pathogens that cause deadly human diseases, such as dengue, zika and malaria. the diversity of the insecticide arsenal has remained stagnant for decades, but the recent rise of insecticide resistance fueled the discovery of novel modes of action, and the power of genomics has reinvigorated this search. this review discusses the importance of comparative and functional insect genomics i ...201728076467
fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression.diapause, also known as dormancy, is a state of arrested development that allows insects to survive unfavorable environmental conditions. diapause-destined insects store large amounts of fat when preparing for diapause. however, the extent to which these accumulated fat reserves influence diapause remains unclear. to address this question, we investigated the function of fatty acid synthase (fas), which plays a central role in lipid synthesis, in stress tolerance, the duration of diapause prepar ...201728071706
avian malaria on madagascar: bird hosts and putative vector mosquitoes of different plasmodium lineages.avian malaria occurs almost worldwide and is caused by haemosporida parasites (plasmodium, haemoproteus and leucocytozoon). vectors such as mosquitoes, hippoboscid flies or biting midges are required for the transmission of these parasites. there are few studies about avian malaria parasites on madagascar but none about suitable vectors.201728057063
the transcriptome of the mosquito aedes fluviatilis (diptera: culicidae), and transcriptional changes associated with its native wolbachia infection.wolbachia is a bacterial endosymbiont that naturally infects a wide range of insect species, and causes drastic changes to host biology. stable infections of wolbachia in mosquitoes can inhibit infection with medically important pathogens such as dengue virus and malaria-causing plasmodium parasites. however, some native wolbachia strains can enhance infection with certain pathogens, as is the case for the mosquito aedes fluviatilis, where infection with plasmodium gallinaceum is enhanced by the ...201728049478
an overview of mosquitoes and emerging arboviral infections in the zagreb area, croatia.mosquito control in the zagreb area has been conducted for many years, whereas the fauna has only been investigated in the last 20 years. so far 30 mosquito species have been detected in the city area. culex pipiens form molestus is the dominant mosquito species in indoor breeding sites. in forested areas and areas exposed to flooding, the active period is early spring and the dominant species are ochlerotatus sticticus, ochlerotatus cantans, ochlerotatus geniculatus and aedes vexans. the eudomi ...201628036307
the diapause program impacts renal excretion and molecular expression of aquaporins in the northern house mosquito, culex pipiens.adult females of the mosquito culex pipiens entering diapause increase sugar water ingestion and reduce evaporative water loss, but how these attributes of the diapause program impact activity of the renal excretory system remains unknown. here we compared the renal excretory capacity of diapausing and non-diapausing females, as well as the molecular expression of aquaporin (aqp) genes that encode channels involved in transporting water and/or small metabolites. baseline urine excretion rates in ...201728034679
piwis go viral: arbovirus-derived pirnas in vector mosquitoes.vector mosquitoes are responsible for transmission of the majority of arthropod-borne (arbo-) viruses. virus replication in these vectors needs to be sufficiently high to permit efficient virus transfer to vertebrate hosts. the mosquito immune response therefore is a key determinant for arbovirus transmission. mosquito antiviral immunity is primarily mediated by the small interfering rna pathway. besides this well-established antiviral machinery, the piwi-interacting rna (pirna) pathway processe ...201628033427
evaluation of some plant fruit extracts for the control of west nile virus vector culex pipiens (diptera: culicidae).the extracts of different parts of plants were found very effective against various pests. the aim of this research was to determine the insecticidal activity of fruit methanol extracts obtained from melia azedarach (meliaceae), phoenix theophrasti (arecaceae), styphnolobium japonicum (fabaceae) and pyracantha coccinea (rosaceae) against the larvae of culex pipiens (diptera: culicidae).201628032112
laboratory evaluation of temephos against anopheles stephensi and culex pipiens larvae in iran.malaria is still a health problem in iran. there are several vector control activities, including indoor residual spraying, using insecticide treated nets and larviciding including temephos. in addition nuisance mosquitos are prevalent in the urban areas. so that evaluation of this species to larvicide will provide a clue for management of vector control activities.201628032103
high insecticides resistance in culex pipiens (diptera: culicidae) from tehran, capital of iran.during recent years transmission of dirofilaria immitis (dog heart worm) by culex pipiens and west nile virus have been reported from iran. the present study was preformed for evaluating the susceptibility status of cx. pipiens collected from capital city of tehran, iran.201628032100
serological and molecular detection of dirofilaria species in stray dogs and investigation of wolbachia dna by pcr in turkey.dirofilaria immitis and dirofilaria repens are the most common species of filarial nematodes described in the dogs. a single-step multiplex pcr was applied to detect and differentiate simultaneously and unequivocally d. immitis and d. repens on dna extracted from canine peripheral blood and besides to detect the seroprevalance of d. immitis by elisa in elazig province, turkey. a pcr detection of the wolbachia, which plays an important role in d. immitis biology and contributes to the inflammator ...201628032096
identification of disease-transmitting mosquitoes: development of species-specific probes for dna chip assay using mitochondrial coi and nd2 genes and ribosomal internal transcribed spacer 2.mosquitoes, which transmit infectious diseases, such as malaria and dengue fever, are harmful to human health. thus, accurate and rapid identification of vectors is a critical step for the control of mosquito-borne diseases. however, phenotypic variations in adults, lack of recognizable features of the immature, and fragility of mosquitoes make identification difficult. molecular approaches have been widely applied to identify mosquitoes, yet these methods have been focused only on the identific ...201728028142
a novel window entry/exit trap for the study of endophilic behavior of mosquitoes.to overcome the limitations of currently used window entry/exit traps, we developed an efficient new glue net entry/exit trap (gnt) that is economical, easily transported and assembled, and can be utilized for a variety of studies which aim to investigate the dynamics of mosquito movements between indoor and outdoor habitats. cage experiments were conducted to determine what percentage of mosquitoes trying to pass through the netting are actually being caught. the gnt caught 97% of female and 98 ...201728027886
shedding light on the grey zone of speciation along a continuum of genomic divergence.speciation results from the progressive accumulation of mutations that decrease the probability of mating between parental populations or reduce the fitness of hybrids-the so-called species barriers. the speciation genomic literature, however, is mainly a collection of case studies, each with its own approach and specificities, such that a global view of the gradual process of evolution from one to two species is currently lacking. of primary importance is the prevalence of gene flow between div ...201628027292
effects of irrigation and rainfall on the population dynamics of rift valley fever and other arbovirus mosquito vectors in the epidemic-prone tana river county, kenya.rift valley fever (rvf) is a mosquito-borne viral zoonosis that is found in most regions of sub-saharan africa, and it affects humans, livestock, and some wild ungulates. outbreaks are precipitated by an abundance of mosquito vectors associated with heavy persistent rainfall with flooding. we determined the impact of flood-irrigation farming and the effect of environmental parameters on the ecology and densities of primary and secondary vectors of the rvf virus (rvfv) in an rvf-epidemic hotspot ...201628011732
artificial diets for mosquitoes.mosquito-borne diseases are responsible for more than a million human deaths every year. modern mosquito control strategies such as sterile insect technique (sit), release of insects carrying a dominant lethal (ridl), population replacement strategies (pr), and wolbachia-based strategies require the rearing of large numbers of mosquitoes in culture for continuous release over an extended period of time. anautogenous mosquitoes require essential nutrients for egg production, which they obtain thr ...201628009851
the effect of west nile virus infection on the midgut gene expression of culex pipiens quinquefasciatus say (diptera: culicidae).the interaction of the mosquito and the invading virus is complex and can result in physiological and gene expression alterations in the insect. the association of west nile virus (wnv) and culex pipiens quinquefasciatus mosquitoes results in measurable changes in gene expression; 22 gene products were shown previously to have altered expression. sequence analysis of one product, cq g1a1, revealed 100% amino acid identity to gram negative bacteria binding proteins (cpqgbp) in cx. p. quinquefasci ...201627999244
isolation and characterization of native bacillus thuringiensis strains from saudi arabia with enhanced larvicidal toxicity against the mosquito vector anopheles gambiae (s.l.).worldwide, mosquito vectors are transmitting several etiological agents of important human diseases, including malaria, causing millions of deaths every year. in saudi arabia, as elsewhere, vector-control is based mostly on chemical insecticides which may be toxic and cause environmental deprivation. here, to support the development of bio-pesticide alternatives, a study was conducted to identify native bacillus thuringiensis (bt) isolates with improved toxicity against the malaria vector, anoph ...201627993165
first report of l1014f-kdr mutation in culex pipiens complex from morocco.mosquitoes of the culex pipiens complex, competent vectors for west nile virus (wnv) and rift valley fever virus (rvfv) are widely targeted by insecticide treatments. the intensive application of chemical insecticides led to the development of resistance in many insects including culex pipiens mosquitoes. the absence of data on resistance mechanisms in morocco allow us to assess the levels of lambda-cyhalothrin resistance and the frequency of the mutated gene l1014f kdr in different forms of cx. ...201627986090
bacteriophage wo can mediate horizontal gene transfer in endosymbiotic wolbachia genomes.phage-mediated horizontal gene transfer (hgt) is common in free-living bacteria, and many transferred genes can play a significant role in their new bacterial hosts. however, there are few reports concerning phage-mediated hgt in endosymbionts (obligate intracellular bacteria within animal or plant hosts), such as wolbachia. the wolbachia-infecting temperate phage wo can actively shift among wolbachia genomes and has the potential to mediate hgt between wolbachia strains. in the present study, w ...201627965627
transcriptional response of silkworm (bombyx mori) eggs to o₂ or hcl treatment.diapause is a common biological phenomenon that occurs in many organisms, including fish, insects, and nematodes. in the silkworm (bombyx mori), diapause generally occurs in the egg stage. treatment with o₂, hcl, or other compounds can prevent egg diapause. here, we characterized the transcriptomic responses of newly laid eggs treated with o₂ or hcl. digital gene expression analysis showed that 610 genes in o₂-treated eggs and 656 in hcl-treated eggs were differentially expressed. of these, 343 ...201627941611
culex pipiens forms and urbanization: effects on blood feeding sources and transmission of avian plasmodium.the wide spread mosquito culex pipiens pipiens have two forms molestus and pipiens which frequently hybridize. the two forms have behavioural and physiological differences affecting habitat requirements and host selection, which may affect the transmission dynamic of cx. p. pipiens-borne diseases.201627931226
first report on human-biting culex pipiens in sweden.culex mosquitoes are vectors of several bird-hosted arboviruses that cause outbreaks in europe, such as sindbis virus and west nile virus. recently, the human-biting form of culex pipiens, cx. pipiens biotype molestus, was found causing big nuisance in a housing cooperative in gothenburg in southern sweden, confirmed by molecular identification. this is the first report of human-biting culex in scandinavia, signalling increased risk of arbovirus infection in northern europe.201627927233
anthropogenic impacts on mosquito populations in north america over the past century.the recent emergence and spread of vector-borne viruses including zika, chikungunya and dengue has raised concerns that climate change may cause mosquito vectors of these diseases to expand into more temperate regions. however, the long-term impact of other anthropogenic factors on mosquito abundance and distributions is less studied. here, we show that anthropogenic chemical use (ddt; dichlorodiphenyltrichloroethane) and increasing urbanization were the strongest drivers of changes in mosquito ...201627922001
the ace-1 locus is amplified in all resistant anopheles gambiae mosquitoes: fitness consequences of homogeneous and heterogeneous duplications.gene copy-number variations are widespread in natural populations, but investigating their phenotypic consequences requires contemporary duplications under selection. such duplications have been found at the ace-1 locus (encoding the organophosphate and carbamate insecticides' target) in the mosquito anopheles gambiae (the major malaria vector); recent studies have revealed their intriguing complexity, consistent with the involvement of various numbers and types (susceptible or resistant to inse ...201627918584
bioactivities of a new pyrrolidine alkaloid from the root barks of orixa japonica.a new pyrrolidine alkaloid named (z)-3-(4-hydroxybenzylidene)-4-(4-hydroxyphenyl)-1-methylpyrrolidin-2-one was isolated from the ethanol extract of the root barks of orixa japonica. the structure of the new alkaloid was elucidated on the basis of nmr and ms analysis. the compound exhibited larvicidal activity against the fourth instar larvae of aedes aegypti (lc50 = 232.09 μg/ml), anopheles sinensis (lc50 = 49.91 μg/ml), and culex pipiens pallens (lc50 = 161.10 μg/ml). the new alkaloid also poss ...201627918451
widespread usutu virus outbreak in birds in the netherlands, 2016.we report a widespread usutu virus outbreak in birds in the netherlands. viral presence had been detected through targeted surveillance as early as april 2016 and increased mortality in common blackbirds and captive great grey owls was noticed from august 2016 onwards. usutu virus infection was confirmed by post-mortem examination and rt-pcr. extensive usutu virus activity in the netherlands in 2016 underlines the need to monitor mosquito activity and mosquito-borne infections in 2017 and beyond ...027918257
circulation of dirofilaria repens and dirofilaria immitis in moldova.over the last two decades, a significant spread of dirofilariasis has been observed in eastern and central europe. however, data on the circulation of dirofilaria spp. in moldova were absent although direct neighbor states reported high incidence rates of human dirofilariasis.201627912786
exposure of the mosquito vector culex pipiens to the malaria parasite plasmodium relictum: effect of infected blood intake on immune and antioxidant defences, fecundity and survival.the intake of a plasmodium-infected blood meal may affect mosquito physiology and a series of trade-offs may occur, in particular between immune defences, reproduction and self-maintenance. we evaluated the cost of exposure to plasmodium in the mosquito vector by investigating the effect of exposure on fecundity and survival and the implication of immune and antioxidant defences in mediating this cost.201627899136
mutations in acetylcholinesterase2 (ace2) increase the insensitivity of acetylcholinesterase to fosthiazate in the root-knot nematode meloidogyne incognita.the root-knot nematode meloidogyne incognita causes severe damage to continuously cropping vegetables. the control of this nematode relies heavily on organophosphate nematicides in china. here, we described resistance to the organophosphate nematicide fosthiazate in a greenhouse-collected resistant population (rp) and a laboratory susceptible population (sp) of m. incognita. fosthiazate was 2.74-fold less toxic to nematodes from rp than that from sp. quantitative real-time pcr revealed that the ...201627897265
do mosquitoes transmit the avian malaria-like parasite haemoproteus? an experimental test of vector competence using mosquito saliva.the life-cycle of many vector-borne pathogens includes an asexual replication phase in the vertebrate host and sexual reproduction in the insect vector. however, as only a small array of parasites can successfully develop infective phases inside an insect, few insect species are competent vectors for these pathogens. molecular approaches have identified the potential insect vectors of blood parasites under natural conditions. however, the effectiveness of this methodology for verifying mosquito ...201627894354
diet-induced nutritional stress and pathogen interference in wolbachia-infected aedes aegypti.the pathogen interference phenotype greatly restricts infection with dengue virus (denv) and other pathogens in wolbachia-infected aedes aegypti, and is a vital component of wolbachia-based mosquito control. critically, the phenotype's causal mechanism is complex and poorly understood, with recent evidence suggesting that the cause may be species specific. to better understand this important phenotype, we investigated the role of diet-induced nutritional stress on interference against denv and t ...201627893736
preparation of ecofriendly formulations containing biologically active monoterpenes with their fumigant and residual toxicities against adults of culex pipiens.different mixtures of monoterpenes (ketone, alcohol, and alkene) were loaded on paper discs and wax and their knockdown activities were evaluated against culex pipiens adults. some individual monoterpenes were also evaluated by residual toxicity technique. citronella oil as a reference was also loaded separately or in combination with monoterpenes on paper discs and wax. the ketone monoterpenes mixture (camphor, menthone, carvone, and fenchone) on paper discs was the most active (kt50 = 17.20 mi ...201627891154
susceptibility profile and metabolic mechanisms involved in aedes aegypti and aedes albopictus resistant to ddt and deltamethrin in the central african republic.aedes aegypti and ae. albopictus are the main epidemic vectors of dengue, chikungunya and zika viruses worldwide. their control during epidemics relies mainly on control of larvae and adults with insecticides. unfortunately, loss of susceptibility of both species to several insecticide classes limits the efficacy of interventions. in africa, where aedes-borne viruses are of growing concern, few data are available on resistance to insecticides. to fill this gap, we assessed the susceptibility to ...201627881148
latitudinal diversity of culex pipiens biotypes and hybrids in farm, peri-urban, and wetland habitats in europe.despite the presence of culex (cx.) pipiens mosquitoes and circulation of west nile virus (wnv), wnv outbreaks have so far not occurred in northern europe. the species cx. pipiens consists of two morphologically identical biotypes, pipiens and molestus, which can form hybrids. until now, population dynamic studies of cx. pipiens have not differentiated between biotypes and hybrids at the european scale, nor have they used comparative surveillance approaches. we therefore aimed to elucidate the r ...201627870890
structure, spatial and temporal distribution of the culex pipiens complex in shanghai, china.culex pipiens molestus was first reported in shanghai in 2010. the population structures and seasonal distributions of culex pipiens subspecies c. p. molestus, culex pipiens pallens, and culex pipiens quinquefasciatus are not well known.201627869687
synthesis and characterization of silver nanoparticles using bacillus amyloliquefaciens and bacillus subtilis to control filarial vector culex pipiens pallens and its antimicrobial activity.culex pipiens pallens are the most common mosquito's vector in asia. in order to protect the people from diseases, the anti-mosquito population is necessary that uses safe and new bio-pesticides such as bacteria-agnps. in our report, we used two kinds of bacteria to synthesize silver nanoparticles to examine the toxic effect on the larvae and pupae of cx. pipiens pallens and also used as antimicrobial activity. the biosynthesis of agnps and its characterization was carried out by uv-vis spectrop ...201627855517
solid-state nmr reveals differential carbohydrate utilization in diapausing culex pipiens.culex pipiens is the mosquito that vectors west nile virus and other human-pathogenic flavivruses in north america. in response to shortened day length and lower temperatures, female cx. pipiense prepares for the diapause by actively feeding on carbohydrates to increase the biosynthesis of glycogen and lipid to store energy for overwintering. the effect of feeding different carbohydrates on glycogen and lipid biosynthesis in diapausing mosquitoes was investigated in vivo using 13c solid-state nm ...201627853281
searching for putative avian malaria vectors in a seasonally dry tropical forest in brazil.haemosporidian parasites of the genera plasmodium and haemoproteus can have detrimental effects on individual birds and populations. despite recent investigations into the distribution and richness of these parasites and their vertebrate hosts, little is known about their dipteran vectors. the neotropics has the highest diversity of mosquitoes in the world, but few studies have tried to identify vectors in this area, hampering the understanding of the ecology of avian malaria in the highly diver ...201627852326
review of west nile virus circulation and outbreak risk in madagascar: entomological and ornithological perspectives.west nile fever (wnf) is a zoonotic disease, occurring nearly globally. in madagascar, west nile virus (wnv) was first detected in 1978 from wild birds and the virus is currently distributed across the island, but no epidemic or epizootic period has been recorded. one fatal human case of wnv infection was reported in 2011, suggesting a "tip of the iceberg" phenomenon of a possible wnf epidemic/epizootic on the island. the main objective of this literature-based survey is to review patterns of wn ...201627849515
downregulation of aedes aegypti chromodomain helicase dna binding protein 7/kismet by wolbachia and its effect on dengue virus replication.dengue virus (denv) is a mosquito-transmitted virus imposing a significant burden on human health around the world. since current control strategies are not sufficient, there is an urgent need to find alternative methods to control denv transmission. it has been demonstrated that introduction of wolbachia pipientis in aedes aegypti mosquitoes can impede denv transmission with the mechanism(s) not fully understood. recently, a number of studies have found the involvement of chromodomain dna bindi ...201627827425
transcriptome analysis of the midgut of the chinese oak silkworm antheraea pernyi infected with antheraea pernyi nucleopolyhedrovirus.the antheraea pernyi nucleopolyhedrovirus (apnpv) is an exclusive pathogen of a. pernyi. the intense interactions between apnpv and a. pernyi cause a series of physiological and pathological changes to a. pernyi. however, no detailed report exists regarding the molecular mechanisms underlying the interactions between apnpv and a. pernyi. in this study, four cdna libraries of the a. pernyi midgut, including two apnpv-infected groups and two control groups, were constructed for transcriptomic anal ...201627820844
west virginia mosquitoes: sequential list by publication, newly found species, corrections, and notes for earlier records.a chronological list of species based on date of publication is provided for confirmed mosquito species in west virginia. five additional newly found species in the state are documented with collection data. two previously misidentified or mislabeled published records are corrected. the number of confirmed species occurring in the state is now 35, and many more species are likely present, but undetected. significant populations of 4 widely distributed known vector species, aedes albopictus , ae. ...201627802407
using next-generation sequencing for dna barcoding: capturing allelic variation in its2.internal transcribed spacer 2 (its2) is a popular dna barcoding marker; however, in some animal species it is hypervariable and therefore difficult to sequence with traditional methods. with next-generation sequencing (ngs) it is possible to sequence all gene variants despite the presence of single nucleotide polymorphisms (snps), insertions/deletions (indels), homopolymeric regions, and microsatellites. our aim was to compare the performance of sanger sequencing and ngs amplicon sequencing in c ...201727799340
distribution of the members of the pipiens assemblage in the sympatric area from argentina: which is where and when?given their medical and veterinary relevance, the members of the pipiens assemblage are a worldwide target of ecological research. the distribution of culex pipiens s.s. and cx. quinquefasciatus converge in buenos aires, argentina, where hybrids have been detected. each member of the assemblage exhibits a distinct eco-physiological behaviour that can affect its efficiency in pathogen transmission. our aim was to identify the environmental drivers for the spatio-temporal distribution of each memb ...201627783720
west nile virus 'circulation' in vojvodina, serbia: mosquito, bird, horse and human surveillance.efforts to detect west nile virus (wnv) in the vojvodina province, northern serbia, commenced with human and mosquito surveillance in 2005, followed by horse (2009) and wild bird (2012) surveillance. the knowledge obtained regarding wnv circulation, combined with the need for timely detection of virus activity and risk assessment resulted in the implementation of a national surveillance programme integrating mosquito, horse and bird surveillance in 2014. from 2013, the system showed highly satis ...201727777104
looking backward, looking forward: the long, torturous struggle with mosquitoes.the american anti-mosquito movement grew out of the discovery of the role of mosquitoes in transferring pathogens and public concern about pest and nuisance mosquitoes in the late 1800s. in the 20th century, organized mosquito control in the united states passed through three eras: mechanical, chemical, and integrated mosquito control. mosquito control in the 21st century faces the challenge of emerging pathogens, invasive mosquito species, and balancing concerns about the environment with effec ...201627775554
molecular expression of aquaporin mrnas in the northern house mosquito, culex pipiens.aquaporins (aqps) are a family of membrane-bound proteins, originally described as water-channels, that broadly exist in prokaryotic and eukaryotic cells. however, some aqps can transport small molecules (e.g. urea, glycerol) along with or preferentially to water. previous work in the mosquitoes aedes aegypti and anopheles gambiae have characterized the molecular expression of one or more aqp genes and shown that they are involved in water homeostasis after blood feeding, tolerance to dehydratio ...201727773635
a review of the mosquito species (diptera: culicidae) of bangladesh.diseases caused by mosquito-borne pathogens remain an important source of morbidity and mortality in bangladesh. to better control the vectors that transmit the agents of disease, and hence the diseases they cause, and to appreciate the diversity of the family culicidae, it is important to have an up-to-date list of the species present in the country. original records were collected from a literature review to compile a list of the species recorded in bangladesh.201627770829
haemocoel injection of pira1b1 to galleria mellonella larvae leads to disruption of the haemocyte immune functions.the bacterium photorhabdus luminescens produces a number of insecticidal proteins to kill its larval prey. in this study, we cloned the gene coding for a binary toxin pira1b1 and purified the recombinant protein using affinity chromatography combined with desalination technology. furthermore, the cytotoxicity of the recombinant protein against the haemocytes of galleria mellonella larvae was investigated. we found that the protein had haemocoel insecticidal activity against g. mellonella with an ...201627734915
genomic evidence for plant-parasitic nematodes as the earliest wolbachia hosts.wolbachia, one of the most widespread endosymbionts, is a target for biological control of mosquito-borne diseases (malaria and dengue virus), and antibiotic elimination of infectious filarial nematodes. we sequenced and analyzed the genome of a new wolbachia strain (wppe) in the plant-parasitic nematode pratylenchus penetrans. phylogenomic analyses placed wppe as the earliest diverging wolbachia, suggesting two evolutionary invasions into nematodes. the next branches comprised strains in sap-fe ...201627734894
rickettsia in mosquitoes, yangzhou, china. 201627729642
resistance in bacteria and indirect beta-lactamase detection in e. coli isolated from culex pipiens detected by matrix-assisted laser desorption ionization time of flight mass spectrometry.the aim of this study was detections of antibiotic resistance and resistance mechanism in bacteria isolated from mosquitos (culex pipiens) living near humans. therefore, antibiotic resistance in bacteria isolated from culex pipiens was investigated by disk diffusion test and mic e-test in this study. maldi-tof mass spectrometry was used for detection of resistant mechanism. in this study, hydrolytic breakdown products after a few hours of incubation of the bacteria isolated from culex pipiens we ...201727726502
finding of anopheles (anopheles) hyrcanus (pallas, 1771) (diptera, culicidae) during the entomological surveillance for west nile virus in umbria, italy.this study reports the mosquito collections conducted in july-august 2013 in castiglione del lago (umbria region, italy) during the entomological surveillance within the west nile national control program. the mosquito collections showed the noteworthy occurrence of anopheles hyrcanus s.l. (n = 156; 35.8% of the whole sample), a relatively rare mosquito species in italy, with molecular analyses con rming that these mosquitoes belong to an. hyrcanus s.s. the same catching site was characterized b ...201627723051
transmission dynamics of the west nile virus in mosquito vector populations under the influence of weather factors in the danube delta, romania.mosquitoes were collected in the danube delta during the active seasons of 2011-2013. for culex spp. mosquitoes, the abundance was calculated. culex pipiens (sensu lato), (s.l.) and culex modestus pools were tested for the presence of west nile virus (wnv) genome, and the maximum likelihood of the infection rate was established. mean daily temperatures and precipitation were obtained for the closest meteorological station. a negative binominal model was used to evaluate linkages between the temp ...201627709311
rapid and high throughput molecular identification of diverse mosquito species by high resolution melting analysis.mosquitoes are a diverse group of invertebrates, with members that are among the most important vectors of diseases. the correct identification of mosquitoes is paramount to the control of the diseases that they transmit. however, morphological techniques depend on the quality of the specimen and often unavailable taxonomic expertise, which may still not be able to distinguish mosquitoes among species complexes (sibling and cryptic species). high resolution melting (hrm) analyses, a closed-tube, ...201627703667
massive yet grossly underestimated global costs of invasive insects.insects have presented human society with some of its greatest development challenges by spreading diseases, consuming crops and damaging infrastructure. despite the massive human and financial toll of invasive insects, cost estimates of their impacts remain sporadic, spatially incomplete and of questionable quality. here we compile a comprehensive database of economic costs of invasive insects. taking all reported goods and service estimates, invasive insects cost a minimum of us$70.0 billion p ...201627698460
goggatomy: a method for opening small cuticular compartments in arthropods for physiological experiments.most sense organs of arthropods are ensconced in small exoskeletal compartments that hinder direct access to plasma membranes. we have developed a method for exposing live sensory and supporting cells in such structures. the technique uses a viscous light cured resin to embed and support the structure, which is then sliced with a sharp blade. we term the procedure a "goggatomy," from the khoisan word for a bug, gogga. to demonstrate the utility of the method we show that it can be used to expose ...201627695420
climate change influences on the global potential distribution of the mosquito culex quinquefasciatus, vector of west nile virus and lymphatic filariasis.rapid emergence of most vector-borne diseases (vbds) may be associated with range expansion of vector populations. culex quinquefasciatus say 1823 is a potential vector of west nile virus, saint louis encephalitis virus, and lymphatic filariasis. we estimated the potential distribution of cx. quinquefasciatus under both current and future climate conditions. the present potential distribution of cx. quinquefasciatus showed high suitability across low-latitude parts of the world, reflecting the c ...201627695107
research contributing to improvements in controlling florida's mosquitoes and mosquito-borne diseases.research on mosquitoes and mosquito-borne diseases has contributed to improvements in providing effective, efficient, and environmentally proper mosquito control. florida has benefitted from several research accomplishments that have increased the state's mosquito control capabilities. research with florida's mosquitoes has resulted in the development of ecologically sound management of mosquito impoundments on florida's east coast. this strategy, called rotational impoundment management (rim), ...201627690112
colour cues: effects of ipomoea plant extract on culex quinquefasciatus say gravid females in choosing oviposition site.the interaction between plants and insects is dynamic, and may favour either the plant or the insect. plant chemicals are deeply implicated in this relationship and influence insect behaviour. here, we investigated the oviposition behaviour response of culex quinquefasciatus mosquitoes based on the colour cues produced by ipomoea cairica leaves extract. in this study, two sets of oviposition choice experiments were conducted: (1) single solution in a cage; and (2) multiple concentration solution ...027688853
arbovirosis and potential transmission blocking vaccines.infectious diseases caused by arboviruses (viruses transmitted by arthropods) are undergoing unprecedented epidemic activity and geographic expansion. with the recent introduction of west nile virus (1999), chikungunya virus (2013) and zika virus (2015) to the americas, stopping or even preventing the expansion of viruses into susceptible populations is an increasing concern. with a few exceptions, available vaccines protecting against arboviral infections are nonexistent and current disease pre ...201627664127
biologically informed individual-based network model for rift valley fever in the us and evaluation of mitigation strategies.rift valley fever (rvf) is a zoonotic disease endemic in sub-saharan africa with periodic outbreaks in human and animal populations. mosquitoes are the primary disease vectors; however, rift valley fever virus (rvfv) can also spread by direct contact with infected tissues. the transmission cycle is complex, involving humans, livestock, and multiple species of mosquitoes. the epidemiology of rvfv in endemic areas is strongly affected by climatic conditions and environmental variables. in this res ...201627662585
relating fitness to long-term environmental variations in natura.quantifying links between ecological processes and adaptation dynamics in natura remains a crucial challenge. many studies have documented the strength, form and direction of selection, and its variations in space and time, but only a few managed to link these variations to their proximal causes. this step is, however, crucial, if we are to understand how the variation in selective pressure affects adaptive allele dynamics in natural settings. we used data from a long-term survey (about 30 years ...201627662519
female adult aedes albopictus suppression by wolbachia-infected male mosquitoes.dengue, chikungunya and zika viruses are pathogens with an increasing global impact. in the absence of an approved vaccine or therapy, their management relies on controlling the mosquito vectors. but traditional controls are inadequate, and the range of invasive species such as aedes albopictus (asian tiger mosquito) is expanding. genetically modified mosquitoes are being tested, but their use has encountered regulatory barriers and public opposition in some countries. wolbachia bacteria can cau ...201627659038
genome sequencing and genetic characterization of culex flavirirus (cxfv) provides new information about its genotypes.culex flavivirus (cxfv) is an insect-specific virus that is widely distributed and primarily infects mosquito species from the genus culex. its hosts include culex tritaeniorhynchus, culex quinquefasciatus, and anopheles sinensis mosquitoes. since its original identification, cxfv has been reported in several countries. despite the increasing number of reports on cxfv, little is known about its genomic characteristics. it is unclear whether the phylogenetic relationships between the strains are ...201627658621
mir-285 targets p450 (cyp6n23) to regulate pyrethroid resistance in culex pipiens pallens.micrornas play critical roles in post-transcriptional regulation of gene expression, which participate in the modulation of almost all of the cellular processes. although emerging evidence indicates that micrornas are related with antineoplastic drugs resistance, whether micrornas are responsible for insecticide resistance in mosquitos is poorly understood. in this paper, we found that mir-285 was significantly upregulated in the deltamethrin-resistant strain of culex pipiens pallens, and overex ...201627651043
comparison of adult mosquito black-light and light-emitting diode traps at three cowsheds located in malaria-endemic areas of the republic of korea.adult mosquito surveillance and field trials evaluated selected commercially available ultraviolet black-light (bl) and light-emitting diode (led) traps at three sites where vivax malaria is endemic from may to october 2015 in northwestern republic of korea. collections totaled 283,929 adult mosquitoes (280,355 [98.74%] females and 3,574 [1.26%] males) comprising 17 species (including six members of the anopheles hyrcanus group) belonging to six genera. the four most predominant female species c ...201627638960
identification of climatic factors affecting the epidemiology of human west nile virus infections in northern greece.climate can affect the geographic and seasonal patterns of vector-borne disease incidence such as west nile virus (wnv) infections. we explore the association between climatic factors and the occurrence of west nile fever (wnf) or west nile neuro-invasive disease (wnnd) in humans in northern greece over the years 2010-2014. time series over a period of 30 years (1979-2008) of climatic data of air temperature, relative humidity, soil temperature, volumetric soil water content, wind speed, and pre ...201627631082
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