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water-induced strong protection against acute exposure to low subzero temperature of adult aedes albopictus.as an important vector of dengue and zika, aedes albopictus has been the fastest spreading invasive mosquitoes in the world over the last 3-4 decades. cold tolerance is important for survival and expansion of insects. ae. albopictus adults are generally considered to be cold-intolerant that cannot survive at subzero temperature. however, we found that ae. albopictus could survive for several hours' exposure to -9 to -19 oc so long as it was exposed with water. median lethal time (lt50) of ae. al ...201930716071
pyrethroids use: threats on metabolic-mediated resistance mechanisms in the primary dengue vector aedes aegypti (diptera: culicidae).the emergence of pyrethroid resistance in aedes aegypti (l.) has limited the success of vector control. early detection of resistance could assist authorities in deciding well-suited control strategies to minimize operational failures of ae. aegypti control. herein, biochemical analysis was performed to investigate the mechanisms involved in pyrethroid resistance in nine populations of indonesian ae. aegypti. enzymes of adult ae. aegypti such as esterases (ests), glutathione-s-transferases (gsts ...201930715464
transcriptional profile of aedes aegypti leucine-rich repeat proteins in response to zika and chikungunya viruses.aedes aegypti (l.) is the primary vector of chikungunya, dengue, yellow fever, and zika viruses. the leucine-rich repeats (lrr)-containing domain is evolutionarily conserved in many proteins associated with innate immunity in invertebrates and vertebrates, as well as plants. we focused on the aaelrim1 and aaeapl1 gene expressions in response to zika virus (zikv) and chikungunya virus (chikv) infection using a time course study, as well as the developmental expressions in the eggs, larvae, pupae, ...201930708982
glycosaminoglycan compositional analysis of relevant tissues in zika virus pathogenesis and in vitro evaluation of heparin as an antiviral against zika virus infection.zika virus (zikv) is an enveloped rna virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women. previously, we established that zikv-ep (envelope protein) binds to human placental chondroitin sulfate (cs), suggesting that cs may be a potential host cell surface receptor in zikv pathogenesis. in this study, we further characterized the gag disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in zikv pathoge ...201930698412
presence of the point mutations val1016gly in the voltage-gated sodium channel detected in a single mosquito from panama.in panama, arboviroses such as dengue fever, and more recently chikungunya fever and zika disease, are transmitted by aedes aegypti and aedes albopictus. their control is based on the elimination of breeding sites and fogging with pyrethroid insecticides. however, one of the significant issues derived from the prolonged use of pyrethroid insecticide is the development of resistance mechanisms, such as knockdown resistance or kdr. the objective of this study was to evaluate the presence of kdr mu ...201930691518
identification of potential inhibitors from pyriproxyfen with insecticidal activity by virtual screening.aedes aegypti is the main vector of dengue fever transmission, yellow fever, zika, and chikungunya in tropical and subtropical regions and it is considered to cause health risks to millions of people in the world. in this study, we search to obtain new molecules with insecticidal potential against ae. aegypti via virtual screening. pyriproxyfen was chosen as a template compound to search molecules in the database zinc_natural_stock (znst) with structural similarity using rocs (rapid overlay of c ...201930691028
factors affecting short-range host-seeking for the yellow fever mosquito (diptera: culicidae).understanding short-range cues (e.g., host odorants, heat, moisture) of host-seeking female aedes aegypti l. is very important for attempts to reduce mosquito bites, to complement current control strategies, and to develop potential spatial repellents. we investigated behavior under semi-field conditions utilizing a new portable uni-port taxis box with a caged chicken host. the combined influences of airflow regimes (0, 1.5, 3, 5, and 6 m/s), distance from host odor (10, 50, 100 cm), host-odor c ...201930689913
predation on eggs of aedes aegypti (diptera: culicidae): temporal dynamics and identification of potential predators during the winter season in a temperate region.in temperate regions, the populations of aedes aegypti (l.) remain in the egg stage during the cold season. the ability of these eggs to survive until the next favorable season is affected by several mortality factors, including the action of predators. in the present study, we analyzed the temporal dynamics of the loss of eggs and identified predators of ae. aegypti eggs along the unfavorable season in a temperate region of argentina. to this end, eggs were exposed in field conditions in pitfal ...201930689912
microbial exopolymer-capped selenium nanowires - towards new antibacterial, antibiofilm and arbovirus vector larvicides?arboviral diseases and microbial pathogens resistant to commercially available drugs are on the rise. herein, a facile microbial-based approach was developed to synthesize selenium nanowires (se nws) using microbial exopolymer (mep) extracted from the bacillus licheniformis (probiotic bacteria). mep-se nws were characterized using uv-visible, xrd, ftir, hr-tem, fe-sem and edx. an uv-visible peak was detected at 330 nm while xrd spectrum data pointed out the crystalline nature of mep-se nws. ftir ...201930685584
complete nmr chemical shift assignments of odorant binding protein 22 from the yellow fever mosquito, aedes aegypti, bound to arachidonic acid.aedes aegypti mosquitoes are the vector for transmission of dengue, zika and chikungunya viruses. these mosquitos feed exclusively on human hosts for a blood meal. previous studies have established that dengue virus infection of the mosquito results in increased expression of the odorant binding proteins 22 and 10 within the mosquito salivary gland and silencing of these genes dramatically reduces blood-feeding behaviors. odorant binding proteins are implicated in modulating the chemosensory per ...201930684234
toxicity and possible mechanisms of action of honokiol from magnolia denudata seeds against four mosquito species.this study was performed to determine the toxicity and possible mechanism of the larvicidal action of honokiol, extracted from magnolia denudata seeds, and its 10 related compounds against third-instar larvae of insecticide-susceptible culex pipiens pallens, aedes aegypti, and aedes albopictus and anopheles sinensis resistant to deltamethrin and temephos. honokiol (lc50, 6.13-7.37 mg/l) was highly effective against larvae of all of the four mosquito species, although the toxicity of the compound ...201930674912
chemical composition, aedes mosquito larvicidal activity, and repellent activity against triatoma rubrofasciata of severinia monophylla leaf essential oil.aedes aegypti and ae. albopictus are key vectors in the spread of arboviruses such as dengue, chikungunya, yellow fever, and zika. triatoma rubrofasciata is an "assassin bug" whose populations and association with humans have dramatically increased and may represent a serious health concern. control of insect vectors is a logical course of action to prevent the spread of these insect-borne infections. this work presents the leaf essential oil composition, mosquito larvicidal activities, and inse ...201930671730
a gut commensal bacterium promotes mosquito permissiveness to arboviruses.mosquitoes are hematophagous vectors that can acquire human viruses in their intestinal tract. here, we define a mosquito gut commensal bacterium that promotes permissiveness to arboviruses. antibiotic depletion of gut bacteria impaired arboviral infection of a lab-adapted aedes aegypti mosquito strain. reconstitution of individual cultivable gut bacteria in antibiotic-treated mosquitoes identified serratia marcescens as a commensal bacterium critical for efficient arboviral acquisition. s. marc ...201930595552
a simple model to predict the potential abundance of aedes aegypti mosquitoes one month in advance.the mosquito aedes (stegomyia) aegypti (l.) is the primary vector of dengue, chikungunya, and zika viruses in the united states. surveillance for adult ae. aegypti is limited, hindering understanding of the mosquito's seasonal patterns and predictions of areas at elevated risk for autochthonous virus transmission. we developed a simple, intuitive empirical model that uses readily available temperature and humidity variables to predict environmental suitability for low, medium, or high potential ...201930594264
density-dependent enhanced replication of a densovirus in wolbachia-infected aedes cells is associated with production of pirnas and higher virus-derived sirnas.the endosymbiotic bacterium wolbachia pipientis has been shown to restrict a range of rna viruses in drosophila melanogaster and transinfected dengue mosquito, aedes aegypti. here, we show that wolbachia infection enhances replication of aedes albopictus densovirus (aaldnv-1), a single stranded dna virus, in aedes cell lines in a density-dependent manner. analysis of previously produced small rnas of aag2 cells showed that wolbachia-infected cells produced greater absolute abundance of virus-der ...201930583288
the aedes aegypti imd pathway is a critical component of the mosquito antifungal immune response.successful infection of the insect body by entomopathogenic fungi is the result of complex molecular interactions between the host and the invading pathogenic fungi. the mosquito antifungal response is multifaceted and is regulated in part by the toll and jak-stat pathways. here, we assessed the role of the imd pathway in the mosquito ae. aegypti antifungal immune response when challenged with one of two entomopathogenic fungi, beauveria bassiana and isaria javanica. imd pathway components of th ...201930582948
green synthesis of silver-nanoparticles from annona reticulata leaves aqueous extract and its mosquito larvicidal and anti-microbial activity on human pathogens.silver nanoparticles play a important role in controlling mosquito population as well as multi drug resistant pathogens without causing much harm to humans. in the present study was focused on green synthesis of silver nanoparticles against dengue causing vector (aedes aegypti) and pathogens affecting humans. the synthesized silver nanoparticle was confirmed using uv- absorption spectrum range obtained at 416 nm, xrd, ftir and hr-tem analysis were used to determine the silver nanoparticle morpho ...201930581768
distinctive regulatory properties of pyruvate kinase 1 from aedes aegypti mosquitoes.female aedes aegypti mosquitoes are vectors of arboviruses that cause diseases of public health significance. the discovery of new metabolic targets is crucial for improving mosquito control strategies. we recently demonstrated that glucose oxidation supports ammonia detoxification in a. aegypti. pyruvate kinase (pk, ec 2.7.1.40) catalyzes the last step of the glycolytic pathway. in most organisms, one or more allosteric effectors control pk activity. however, the kinetic properties and structur ...201930578824
production of tetravalent dengue virus envelope protein domain iii based antigens in lettuce chloroplasts and immunologic analysis for future oral vaccine development.dengue fever is a mosquito (aedes aegypti) -transmitted viral disease that is endemic in more than 125 countries around the world. there are four serotypes of the dengue virus (denv 1-4) and a safe and effective dengue vaccine must provide protection against all four serotypes. to date, the first vaccine, dengvaxia (cyd-tdv), is available after many decades' efforts, but only has moderate efficacy. more effective and affordable vaccines are hence required. plants offer promising vaccine producti ...201930578710
the tudor protein veneno assembles the ping-pong amplification complex that produces viral pirnas in aedes mosquitoes.piwi-interacting rnas (pirnas) comprise a class of small rnas best known for suppressing transposable elements in germline tissues. the vector mosquito aedes aegypti encodes seven piwi genes, four of which are somatically expressed. this somatic pirna pathway generates pirnas from viral rna during infection with cytoplasmic rna viruses through ping-pong amplification by the piwi proteins ago3 and piwi5. yet, additional insights into the molecular mechanisms mediating non-canonical pirna producti ...201930566680
container size alters the outcome of interspecific competition between aedes aegypti (diptera: culicidae) and aedes albopictus.aedes aegypti l. and aedes albopictus skuse co-occur in a variety of water-filled containers where they compete for resources. larvae of ae. albopictus skuse often outcompete those of ae. aegypti l., but variation in biotic and abiotic parameters can modify the outcome of this interspecific competition. we tested whether container size can alter the magnitude and direction of intra- and interspecific competition by rearing three ae. aegypti and ae. albopictus larval combinations (100:0, 50:50 an ...201930566608
bioactivity of wild carrot (daucus carota, apiaceae) essential oil against mosquito larvae.invasive alien plants wreak havoc on native ecosystems and using them as a source of biopesticides could improve their management. we examined the toxicity of essential oil of wild carrot (also known as 'queen anne's lace', daucus carota linnaeus), an aggressive invader throughout the united states, against aedes aegypti l., culex pipiens l., and culex restuans theobald larvae. comparisons were made between essential oil extracted from umbels of local populations of wild carrot versus a commerci ...201930566578
mechanisms of pyrethroid resistance in aedes (stegomyia) aegypti from colombia.in colombia aedes (stegomyia) aegypti is the main vector of urban arboviruses such as dengue, chikungunya and zika. this urban mosquito has a well-established capacity to develop insecticide resistance to different types of insecticides (pyrethroids, organochlorides, organophosphates), using multiple resistance mechanisms. an understanding of ongoing resistance mechanisms is critical to determining the activities of vector control programs. in order to identify the biochemical and molecular mech ...201930552882
proteins, transcripts, and genetic architecture of seminal fluid and sperm in the mosquito aedes aegypti.the yellow fever mosquito, aedes aegypti,, transmits several viruses causative of serious diseases, including dengue, zika, and chikungunya. some proposed efforts to control this vector involve manipulating reproduction to suppress wild populations or to replace them with disease-resistant mosquitoes. the design of such strategies requires an intimate knowledge of reproductive processes, yet our basic understanding of reproductive genetics in this vector remains largely incomplete. to accelerate ...201930552291
seroprevalence of zika virus among asymptomatic pregnant mothers and their newborns in the najran region of southwest saudi arabia.zika virus (zikv) is a teratogenic flavivirus that can cause microcephaly. its main vector, aedes aegypti, has been previ.ously identified in saudi arabia, but no zikv infection has yet been reported. nevertheless, the country is at risk from zikv because it receives many travelers throughout the year, including pilgrims from zikv-endemic countries.201930531174
co-occurrence of v1016i and f1534c mutations in the voltage-gated sodium channel and resistance to pyrethroids in aedes aegypti (l.) from the colombian caribbean region.knockdown resistance is conferred primarily by non-synonymous mutations that reduce pyrethroids binding to voltage-gated sodium channels. in 2014, kdr mutation v1016i in aedes aegypti populations resistant to pyrethroids was reported for the first time in colombiα, in 2016 another kdr mutation, f1534c, and in 2018 the mutation v419l. nine populations of a. aegypti, previously characterized as being resistant to λ-cyhalothrin, deltamethrin, cyfluthrin and permethrin, were used for this study. gen ...201930520256
differential suppression of persistent insect specific viruses in trans-infected wmel and wmelpop-cla aedes-derived mosquito lines.wolbachia suppresses the replication of +ssrna viruses such as dengue and zika viruses in aedes aegypti mosquitoes. however, the range of viruses affected by this endosymbiont is yet to be explored. recently, novel insect-specific viruses (isvs) have been described from numerous mosquito species and mosquito-derived cell lines. cell-fusing agent virus (flaviviridae) and phasi charoen-like virus (bunyaviridae) persistently infect the ae. aegypti cell line aag2 which has been used for experimental ...201930503908
[new altitudinal registration of aedes aegypti in the region of piura, peru]. 201930517515
coinfection of chikungunya and dengue viruses: a serological study from north western region of punjab, india.dengue and chikungunya (chik) infections appear to be increasing in all parts of india. aedes aegypti mosquitoes are common vectors for dengue virus (denv) and chik virus (chikv). in areas where both viruses cocirculate, they can be transmitted together. there are very few studies discussing the dengue-chik coinfection from punjab region of india. the present study was undertaken to study the clinical features of dengue-chik coinfection and compare with monoinfection.201930498319
volatile composition and biological activities of the leaf essential oil from zanthoxylum limoncello grown in oaxaca, méxico.zanthoxylum limoncello is a native plant from southern mexico which is used as a timber source, condiment and as a traditional medicine. herein, we report on the volatile content of the leaf essential oil and its biological activities. the annual essential oils (2015-2018) contained volatile organic compounds which exhibited a moderate growth inhibitory activity against h. pylori atcc 53504 (mic 121.4-139.7 μg ml-1 ), 26695 (mic 85.5-94.9 μg ml-1 ) and j99 (mic 94.7-110.4 μg ml-1 ). these hydrod ...201930512233
saccharomyces cerevisiae (baker's yeast) as an interfering rna expression and delivery system.the broad application of rna interference for disease prevention is dependent upon the production of dsrna in an economically feasible, scalable, and sustainable fashion, as well as the identification of safe and effective methods for rna delivery. current research has sparked interest in the use of saccharomyces cerevisiae for these applications. this review examines the potential for commercial development of yeast interfering rna expression and delivery systems. s. cerevisiae is a genetic mod ...201930474529
ecotoxicity response of chlorpyrifos in aedes aegypti larvae and lactuca sativa seeds after uv/h2o2 and uvc oxidation.chlorpyrifos (cp) is an organophosphate pesticide widely used in agriculture known to cause neurological and immunological effects in addition to interfering in the reproduction and development of organisms. in this study, cp degradation by uv/h2o2 process and uvc radiation was investigated, and the ecotoxicity and phytotoxicity was evaluated using bioassays of aedes aegypti larvae and lactuca sativa seeds. cp degradation was monitored by hplc-dad, and kinetic parameters were calculated for all ...201930471582
autocidal gravid ovitrap incorporation with attractants for control of gravid and host-seeking aedes aegypti (diptera: culicidae).aedes aegypti linnaeus (diptera: culicidae) is a primary vector of multiple arboviruses including zika, dengue, chikungunya, and yellow fever. after incorporating bg lure or bg lure + octenol, the cdc's autocidal gravid ovitrap (ago) was more effective at collecting and controlling host-seeking female ae. aegypti than gravid female mosquitoes. the addition of octenol to the ago did not increase the number of female mosquitoes captured by the ago, compared with the ago alone. the ago baited with ...201930462293
monitoring insecticide resistance profiles of aedes aegypti (diptera: culicidae) in the sunda islands of indonesia based on diagnostic doses of larvicides.this study was conducted to monitor the susceptibility status of aedes aegypti (linnaeus) larvae in the sunda islands of indonesia against various organophosphates and organochlorines. larval bioassay was performed in accordance with the world health organization standard protocol. field-collected and reference strains of ae. aegypti larvae were tested against diagnostic doses of eight larvicides belonging to organophosphates and organochlorines, namely bromophos (0.050 mg/liter), chlopyrifos (0 ...201930462258
past, present and future of aedes aegypti in its south american southern distribution fringe: what do temperature and population tell us?aedes aegypti (diptera: culicidae) (linnaeus) is currently the major threat among arbovirus vectors in the americas. we examined its past, present, and future distribution patterns in the south american fringe in association with environmental and demographic variables at two spatial scales. we updated the database of the occurrence of ae. aegypti per locality and modelled by glmm the past occurrence (until 2000) and its expansion (2001-2017) as a function of air temperature, precipitation, alti ...201930458122
water channel activity of putative aquaporin-6 present in aedes aegypti.aquaporins (aqps) are integral membrane channels that facilitate the bidirectional transport of water and sometimes other small solutes across biological membranes. aqps are important in mediating environmental adaptations in mosquitoes and are considered as a novel target for the development of effective insecticides against mosquitoes. here, we expressed aedes aegypti aqp6 ( aaaqp6) in human embryonic kidney (hek) 293 cells and analyzed the water permeability by a conventional swelling assay, ...201930456765
isolation and growth inhibition potential of entomopathogenic nematodes against three public health important mosquito vectors.the prevalence of mosquito vector borne diseases and the resistance of mosquitoes to conventional pesticides have been of important public concern to the mosquito endemic countries. present study was conducted to identify the native bio-larvicidal potential of the entomopathogenic nematodes; steinernema siamkayai (kpr-4) heterohabditis indica (kpr-8), steinernema glaseri and steinernema abbasi. the isolated nematodes were subsequently cultured and evaluated their larvicidal potential against the ...201930414843
preparation and use of a yeast shrna delivery system for gene silencing in mosquito larvae.the mosquito genome projects facilitated research in new facets of mosquito biology, including functional genetic studies in the dengue and zika virus vector aedes aegypti and the primary african malaria vector anopheles gambiae. rna interference (rnai) has facilitated gene silencing experiments in both of these disease vector mosquito species and could one day be applied as a new method of vector control. here, we describe a procedure for the genetic engineering of saccharomyces cerevisiae (bak ...201930414120
phylogeography and invasion history of aedes aegypti, the dengue and zika mosquito vector in cape verde islands (west africa).aedes-borne arboviruses have spread globally with outbreaks of vast impact on human populations and health systems. the west african archipelago of cape verde had its first outbreak of dengue in 2009, at the time the largest recorded in africa, and was one of the few african countries affected by the zika virus epidemic. aedes aegypti was the mosquito vector involved in both outbreaks. we performed a phylogeographic and population genetics study of a. aegypti in cape verde in order to infer the ...201931548858
panophthalmitis associated with scleral necrosis in dengue hemorrhagic fever.dengue is a mosquito-borne flavivirus disease affecting humans. the aedes aegypti mosquito spreads it. ophthalmic manifestations of dengue range from subconjunctival hemorrhage to optic neuropathy. panophthalmitis in dengue fever is a rare finding. we report a case of a 22-year-old male having dengue fever, who presented with pain, redness, swelling and loss of vision in his right eye. he was diagnosed as panophthalmitis with subretinal hemorrhage and required right eye evisceration.201931546563
the efficiency of a new automated mosquito larval counter and its impact on larval survival.to achieve consistent and standardized rearing for mosquito immature stages, it is crucial to control the initial number of larvae present in each larval tray. in addition, maintaining an optimal and synchronized development rate of larvae is essential to maximize the pupal production and optimize male sorting in a mass-rearing setting. manual counting is labor intensive, time consuming and error prone. therefore, this study aimed to investigate the use of a customized automated counter for the ...201931092868
infection of aedes albopictus mosquito c6/36 cells with the wmelpop strain of wolbachia modulates dengue virus-induced host cellular transcripts and induces critical sequence alterations in the dengue viral genome.dengue virus (denv) causes frequent epidemics infecting ∼390 million people annually in over 100 countries. there are no approved vaccines or antiviral drugs for treatment of infected patients. however, there is a novel approach to control denv transmission by the mosquito vectors, aedes aegypti and aedes albopictus, using the wolbachia symbiont. the wmelpop strain of wolbachia suppresses denv transmission and shortens the mosquito life span. however, the underlying mechanism is poorly understoo ...201931092581
microencapsulation of citrus grandis peel oil using interfacial precipitation chemistry technique for repellent application.essential oil of citrus family plant is known to have repellent effect against mosquito. unfortunately, due to its high volatility effect, its repellency effect was compromised. the incorporation of essential oil in a microencapsulation formulation has been shown to help improve the stability and potency of the repellent. in this study, citrus grandis peel oil (cgpo) was encapsulated by using the interfacial precipitation chemistry technique. the microencapsulated cgpo was then formulated into l ...201931089355
larvicidal, adulticidal, and oviposition-deterrent activity of piper betle l. essential oil to aedes aegypti.aedes aegypti is a primary vector of many arthropod-borne diseases. one of the diseases, dengue fever, is an endemic disease in indonesia causing high mortalities for decades. there are no preventive and specific treatments for dengue so far. therefore, prevention of this disease largely depends on the mosquito control. since resistance to chemical insecticides occurred worldwide, the study on alternate and new mosquito insecticides are mandatory. this study aimed to demonstrate the effect of es ...201931089304
efficacy of the in2care® auto-dissemination device for reducing dengue transmission: study protocol for a parallel, two-armed cluster randomised trial in the philippines.mosquito-borne viruses are imposing an ever increasing health burden worldwide. in addition to the recent zika and chikungunya virus epidemics, dengue viruses have become the fastest growing problem with a 40-fold increase in the number of reported cases over the past five decades. current mosquito control techniques involving larval source reduction, larviciding, and space spray of adulticides are costly, laborious, and of debatable efficacy. there remains an urgent need for the development of ...201931088515
linking nutrient stoichiometry to zika virus transmission in a mosquito.food quality and quantity serve as the basis for cycling of key chemical elements in trophic interactions; yet the role of nutrient stoichiometry in shaping host-pathogen interactions is under appreciated. most of the emergent mosquito-borne viruses affecting human health are transmitted by mosquitoes that inhabit container systems during their immature stages, where allochthonous input of detritus serves as the basal nutrients. quantity and type of detritus (animal and plant) were manipulated i ...201931227906
lichen parmelia sulcata mediated synthesis of gold nanoparticles: an eco-friendly tool against anopheles stephensi and aedes aegypti.the gold nanoparticles (aunps) were synthesized using the lichen parmelia sulcata extract (pse) and characterized. the peaks of ultraviolet spectrophotometer and fourier transmission infrared confirmed the formation of nanoparticles and the bioactive compounds of the lichen being responsible for reducing and capping of the particles. the face-centered cubic particles were determined by xrd peaks at 111, 200, 220, and 311. the elemental composition and spherical shape of aunps were confirmed by e ...201931218582
how do trait-mediated non-lethal effects of predation affect population-level performance of mosquitoes?non-lethal, trait-mediated effects of predation impact prey behavior and life-history traits. studying how these effects in turn influence prey demography is crucial to understand prey life-history evolution. mosquitoes are important vectors that claim several million lives every year worldwide by transmitting a range of pathogens. several ecological factors affect life-history traits of both larval and adult mosquitoes, creating effects that cascade to population-level consequences. few studies ...201931218216
community composition and year-round abundance of vector species of mosquitoes make miami-dade county, florida a receptive gateway for arbovirus entry to the united states.vector-borne diseases are a heavy burden to human-kind. global warming and urbanization have a significant impact on vector-borne disease transmission, resulting in more severe outbreaks, and outbreaks in formerly non-endemic areas. miami-dade county, florida was the most affected area in the continental united states during the 2016 zika virus outbreak. miami is an important gateway and has suitable conditions for mosquitoes year-round. therefore, it was critical to establish and validate a sur ...201931217547
development of cs-tpp-dsrna nanoparticles to enhance rnai efficiency in the yellow fever mosquito, aedes aegypti.mosquito-borne diseases are a major threat to human health and are responsible for millions of deaths globally each year. vector control is one of the most important approaches used in reducing the incidence of these diseases. however, increasing mosquito resistance to chemical insecticides presents challenges to this approach. therefore, new strategies are necessary to develop the next generation vector control methods. because of the target specificity of dsrna, rnai-based control measures are ...201931217512
mosquitocidal chips containing the insect growth regulator pyriproxyfen for control of aedes aegypti (diptera: culicidae).aedes aegypti were exposed to water treated with mosquitocidal chips containing the insecticide pyriproxyfen in a polymer formulation. chips were tested under different conditions; different water volumes, in containers made of different material, and in water with different levels of organic matter. treated chips caused 100% mortality of ae. aegypti during their pupal stage independent of size or type of container, and the mount of organic matter contained in the water to which the mosquito lar ...201931216627
oviposition preferences of the mosquito aedes aegypti linnaeus, 1762 (culicidae): an urban environment bioassay.the establishment of an invasive species depends on reproductive success and dispersion capability in the new environment. one of the striking examples of invasion in urban environments is the mosquito aedes aegypti linnaeus, 1762 (culicidae). the success of this species is primarily attributed to its ability to colonize urban environments, and some of the important adaptive strategies associated with this ability is the preference for humans as a blood source and intense occupation of residenti ...201931213206
inferring who-infected-whom-where in the 2016 zika outbreak in singapore-a spatio-temporal model.singapore experienced its first known zika outbreak in 2016. given the lack of herd immunity, the suitability of the climate for pathogen transmission, and the year-round presence of the vector- aedes aegypti-zika had the potential to become endemic, like dengue. guillain-barré syndrome and microcephaly are severe complications associated elsewhere with zika and the risk of these complications makes understanding its spread imperative. we investigated the spatio-temporal spread of locally transm ...201931213175
asymptomatic dengue virus infections, cambodia, 2012-2013.we investigated dengue virus (denv) and asymptomatic denv infections in rural villages of kampong cham province, cambodia, during 2012 and 2013. we conducted perifocal investigations in and around households for 149 denv index cases identified through hospital and village surveillance. we tested participants 0.5-30 years of age by using nonstructural 1 rapid tests and confirmed denv infections using quantitative reverse transcription pcr or nonstructural 1-capture elisa. we used multivariable po ...201931211672
live calcium imaging of aedes aegypti neuronal tissues reveals differential importance of chemosensory systems for life-history-specific foraging strategies.the mosquito aedes aegypti has a wide variety of sensory pathways that have supported its success as a species as well as a highly competent vector of numerous debilitating infectious pathogens. investigations into mosquito sensory systems and their effects on behavior are valuable resources for the advancement of mosquito control strategies. numerous studies have elucidated key aspects of mosquito sensory systems, however there remains critical gaps within the field. in particular, compared to ...201931208328
the difference of sve-cadherin levels between dengue hemorrhagic fever patients with shock and without shock.dengue virus infection is an infectious disease caused by the dengue virus and transmitted by the aedes aegypti mosquito. dengue virus (den-v) consists of 4 serotypes, namely den-1, den-2, den-3, and den-4. the most feared result of dhf is death. death in children is caused by hypovolemic shock due to plasma leakage from intravascular to extravascular space due to endothelial dysfunction.201931592274
a peridomestic aedes malayensis population in singapore can transmit yellow fever virus.the case-fatality rate of yellow fever virus (yfv) is one of the highest among arthropod-borne viruses (arboviruses). although historically, the asia-pacific region has remained free of yfv, the risk of introduction has never been higher due to the increasing influx of people from endemic regions and the recent outbreaks in africa and south america. singapore is a global hub for trade and tourism and therefore at high risk for yfv introduction. effective control of the main domestic mosquito vec ...201931589616
identification of zika virus in immature phases of aedes aegypti and aedes albopictus: a surveillance strategy for outbreak anticipation.a progressive increase in the circulation of arboviruses in tropical countries has been observed, accounting for 700,000 yearly deaths in the world. the main objective of this article was to identify the presence of zika (zikv), dengue (denv), and chikungunya (chikv) viruses in immature stages of aedes aegypti and ae. albopictus. household collections of immature phases of the vectors were carried out in the years 2015 and 2016. a total of 2902 dwellings were visited and the rate of infestation ...201931721902
genomic epidemiology supports multiple introductions and cryptic transmission of zika virus in colombia.colombia was the second most affected country during the american zika virus (zikv) epidemic, with over 109,000 reported cases. despite the scale of the outbreak, limited genomic sequence data were available from colombia. we sought to sequence additional samples and use genomic epidemiology to describe zikv dynamics in colombia.201931718580
the influence of new surveillance data on predictive species distribution modeling of aedes aegypti and aedes albopictus in the united states.the recent emergence or reemergence of various vector-borne diseases makes the knowledge of disease vectors' presence and distribution of paramount concern for protecting national human and animal health. while several studies have modeled aedes aegypti or aedes albopictus distributions in the past five years, studies at a large scale can miss the complexities that contribute to a species' distribution. many localities in the united states have lacked or had sporadic surveillance conducted for t ...201931718099
chemical compositions and mosquito larvicidal activities of essential oils from piper species growing wild in central vietnam.mosquitoes are the deadliest animals on earth and are the vectors of several neglected tropical diseases. recently, essential oils have emerged as potential renewable, cost-effective, and environmentally benign alternatives to synthetic pesticides for control of mosquitoes. in this work, thirteen species of piper were collected from different areas of central vietnam. the essential oils were obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. the essential oils we ...201931717867
resistance status of aedes aegypti to deltamethrin, malathion, and temephos in ecuador.in ecuador, the status of insecticide resistance for aedes aegypti, the principal arboviral vector in the country, has not been previously evaluated. the aim of this research was to describe the resistance status of ae. aegypti to the principal insecticides used for vector control in provinces with high reports of arboviral clinical cases. this was a descriptive study performed on ae. aegypti collected from 2016 to 2017 in 14 localities of ecuador. the larvae were reared and tested using bioassa ...201931442135
a survey for aedes aegypti in delaware and the virus-positive pool rates of aedes albopictus and aedes triseriatus for west nile and zika viruses.the introduction of zika virus to the usa in 2015 engendered heightened interest in its known vectors. aedes aegypti is the primary vector, with ae. albopictus considered a potential secondary vector, together with several other possible marginal vectors. in delaware, ae. aegypti has been collected rarely, but no breeding populations were detected during past intensive statewide surveillance efforts. however, there is an abundance of ae. albopictus statewide. both species are container breeders ...201931442131
field efficacy trials of aerial ultra-low-volume application of insecticides against caged aedes aegypti in mexico.we evaluated the efficacy of aerial ultra-low-volume (ulv) insecticide spraying in field bioassays with caged aedes aegypti in may 2017 in puerto vallarta, jalisco, mexico. the insecticides tested included an organophosphate (mosquitocida uno ulv) and a neonicotinoid-pyrethroid combination (cielo). two ae. aegypti populations were evaluated: a field pyrethroid-resistant local strain (puerto vallarta) and an insecticide-susceptible laboratory strain (new orleans). knockdown after 1 h by both prod ...201931442128
evaluation and comparison of spray equipment for indoor residual spraying.the world health organization (who) has recently recommended indoor residual spraying (irs) as part of a vector control strategy to combat aedes-borne diseases, including dengue, chikungunya, and zika viruses. hand compression sprayers have been used in malaria prevention and control programs worldwide since the 1950s and are a standard for irs application. however, there are technological advances that should be considered to improve irs application (e.g., flow-control valves, rechargeable-batt ...201931442127
semi-field evaluation of modified 00zzzero® traps with sticky paper to increase the collection efficacy of gravid aedes aegypti.the 00zzzero® trap (0z) is a small black plastic container with permethrin and pyriproxyfen, designed and marketed to both attract and kill gravid aedes mosquitoes (unmodified trap ([umt] or modified trap [mt]). the 0z trap without the insecticide was modified with addition of a sticky paper, coated with an adhesive glue (mt) and evaluated for collection efficacy of released gravid mosquito, aedes aegypti (l.) in outdoor screened enclosures in st. augustine, in northeastern florida. the mean num ...201931442126
influence of sex ratio and density on the comparative repellency of ir3535® against caged aedes, anopheles, and culex mosquitoes.studies on topical repellent efficacy conducted with caged mosquitoes in the laboratory are important to both the development and regulation of insect repellents. guidelines for laboratory studies stipulate specific densities, sex ratios, and biting rates, whereas those for field studies are governmentally required before a promising repellent can be registered for human use. these protocols stipulate minimum biting rates alone. relatively little is known, however, about the influence of mosquit ...201931441773
dynamics and diversity of bacteria associated with the disease vectors aedes aegypti and aedes albopictus.aedes aegypti and aedes albopictus develop in the same aquatic sites where they encounter microorganisms that influence their life history and capacity to transmit human arboviruses. some bacteria such as wolbachia are currently being considered for the control of dengue, chikungunya and zika. yet little is known about the dynamics and diversity of aedes-associated bacteria, including larval habitat features that shape their tempo-spatial distribution. we applied large-scale 16s rrna amplicon se ...201931434963
systematic identification and characterization of aedes aegypti long noncoding rnas (lncrnas).long noncoding rnas (lncrnas) play diverse roles in biological processes. aedes aegypti (ae. aegypti), a blood-sucking mosquito, is the principal vector responsible for replication and transmission of arboviruses including dengue, zika, and chikungunya virus. systematic identification and developmental characterisation of ae. aegypti lncrnas are still limited. we performed genome-wide identification of lncrnas, followed by developmental profiling of lncrna in ae. aegypti. we identified a total o ...201931434910
copper(ii)-binding induces a unique polyproline type ii helical structure within the ion-binding segment in the intrinsically disordered f-domain of ecdysteroid receptor from aedes aegypti.reproduction of the dominant vector of zika and dengue diseases, aedes aegypti mosquito, is controlled by an active heterodimer complex composed of the 20-hydroxyecdysone receptor (ecr) and ultraspiracle protein. although a. aegypti ecr shares the structural and functional organization with other nuclear receptors, its c-terminus has an additional long f domain (aafecr). recently, we showed that the full length aafecr is intrinsically disordered with the ability to specifically bind divalent met ...201931433630
identification of anti-flaviviral drugs with mosquitocidal and anti-zika virus activity in aedes aegypti.zika virus (zikv), an emerging arbovirus belonging to the genus flavivirus, is transmitted by aedes mosquitoes. zikv infection can cause microcephaly of newborn babies and guillain-barré syndrome in adults. because no licensed vaccine or specific antiviral treatment is available for zikv infection, the most commonly used approach to control the spread of zikv is suppression of the mosquito vector population. a novel proposed strategy to block arthropod virus (arbovirus) transmission is based on ...201931430351
detecting wmel wolbachia in field-collected aedes aegypti mosquitoes using loop-mediated isothermal amplification (lamp).the world mosquito program uses wolbachia pipientis for the biocontrol of arboviruses transmitted by aedes aegypti mosquitoes. diagnostic testing for wolbachia in laboratory colonies and in field-caught mosquito populations has typically employed pcr. new, simpler methods to diagnose wolbachia infection in mosquitoes are required for large-scale operational use.201931416478
the major oligosaccharyl transferase complex genes are not involved in dengue virus replication in aedes aegypti mosquitoes.replication of the dengue virus (denv) genome occurs in a vesicle in the endoplasmic reticulum by a complex of host and viral proteins. two host proteins, stt3a and stt3b, as members of the oligosaccharyl transferase complex, have been implicated in playing structural roles in the vesicle in mammalian cells, and the absence of these proteins has been shown to decrease denv replication. aedes aegypti is the main vector of the virus and has been used previously as a model organism to study mosquit ...201931414286
antibiotic resistant bacteria and commensal fungi are common and conserved in the mosquito microbiome.the emerging and increasing prevalence of bacterial antibiotic resistance is a significant public health challenge. to begin to tackle this problem, it will be critical to not only understand the origins of this resistance but also document environmental reservoirs of antibiotic resistance. in this study we investigated the possibility that both colony and field caught mosquitoes could harbor antibiotic resistant bacteria. specifically, we characterized the antibiotic resistant bacterial populat ...201931412044
midgut barriers prevent the replication and dissemination of the yellow fever vaccine in aedes aegypti.to be transmitted to vertebrate hosts via the saliva of their vectors, arthropod-borne viruses have to cross several barriers in the mosquito body, including the midgut infection and escape barriers. yellow fever virus (yfv) belongs to the genus flavivirus, which includes human viruses transmitted by aedes mosquitoes, such as dengue and zika viruses. the live-attenuated yfv-17d vaccine has been used safely and efficiently on a large scale since the end of world war ii. early studies have shown, ...201931412040
nature of complex network of dengue epidemic as a scale-free network.dengue epidemic is a dynamic and complex phenomenon that has gained considerable attention due to its injurious effects. the focus of this study is to statically analyze the nature of the dengue epidemic network in terms of whether it follows the features of a scale-free network or a random network.201931406610
zika virus detection, isolation and genome sequencing through culicidae sampling during the epidemic in vitória, espírito santo, brazil.zika virus (zikv) has been isolated from many mosquito species in nature, but it is believed that the main vectors in urban environments are species of the genus aedes. here, we detected and isolated zikv in samples from aedes aegypti, aedes taeniorhynchus and culex quinquefasciatus, collected during the zika epidemic in vitória, southeast brazil. using quantitative real-time polymerase chain reaction, zikv detection was performed in mosquito samples collected from february to april 2016.201931068218
determination of the spatial susceptibility to yellow fever using a multicriteria analysis.the outbreak of sylvatic yellow fever (syf) in humans during 2016-2017 in brazil is one of the greatest in the history of the disease. the occurrence of the disease in areas with low vaccination coverage favoured the dissemination of the disease; therefore, it is necessary to identify the areas vulnerability to the yf virus (yfv) to assist in the adoption of preventive measures.201931066755
embryonic development and egg viability of wmel-infected aedes aegypti.aedes aegypti is a major disease vector in urban habitats, involved in the transmission of dengue, chikungunya and zika. despite innumerous attempts to contain disease outbreaks, there are neither efficient vaccines nor definite vector control methods nowadays. in recent years, an innovative strategy to control arboviruses, which exploits the endosymbiotic bacterium wolbachia pipientis, emerged with great expectations. the success of the method depends on many aspects, including wolbachia's cyto ...201931060581
arboviral screening of invasive aedes species in northeastern turkey: west nile virus circulation and detection of insect-only viruses.the recent reports of aedes aegypti and ae. albopictus populations in turkey, in parallel with the territorial expansion identified in several surrounding countries, have raised concerns about the establishment and re-establishment of these invasive aedes mosquitoes in turkey. this cross-sectional study was performed to detect aedes aegypti and ae. albopictus in regions of recent incursions, and screen for viral pathogens known to be transmitted elsewhere by these species.201931059502
dengue virus: a review on advances in detection and trends - from conventional methods to novel biosensors.dengue virus is an important arbovirus infection which transmitted by the aedes female mosquitoes. the attempt to control and early detection of this infection is a global public health issue at present. because of the clinical importance of its detection, the main focus of this review is on all of the methods that can offer the new diagnosis strategies. the advantages and disadvantages of reported methods have been discussed comprehensively from different aspects like biomarkers type, sensitivi ...201931055654
evidence for infection but not transmission of zika virus by aedes albopictus (diptera: culicidae) from spain.a number of mosquito-borne viruses such as dengue virus (denv), usutu virus (usuv), west nile virus (wnv) are autochthonously transmitted in europe and six invasive mosquito species have been detected in this temperate region. this has increased the risk for the emergence of further mosquito-borne diseases. however, there is a paucity of information on whether european populations of invasive mosquito species are competent to transmit arboviruses. in this study, the susceptibility of aedes albop ...201931053164
an evaluation of efficacy of the auto-dissemination technique as a tool for aedes aegypti control in madeira, portugal.the frequency and intensity of arboviral epidemics is steadily increasing and posing an intractable public health burden. current vector control methods are proving ineffectual and despite progress in the development of high technology approaches, there is an urgent need for the development of tools for immediate implementation. several studies suggest that the auto-dissemination of pyriproxyfen (ppf) is a promising new approach to larviciding although there is little detail on the conditions un ...201931053095
a facile and rapid method for green synthesis of achyranthes aspera stem extract-mediated silver nano-composites with cidal potential against aedes aegypti l.aedes aegypti l. is the primary vector associated with transmission of globally concerned diseases; zika, yellow fever, dengue and chikungunya. present study investigates an efficient, alternative and comparative approach for mosquito control which is safe to environment and non-target organisms. the silver nano-composites (agncs) were synthesized from the aqueous stem extract of achyranthes aspera (aase) using different concentration of aqueous silver nitrate (agno3). the synthesis was tracked ...201931048994
voltages and resistances of the anterior malpighian tubule of drosophila melanogaster.the small size of malpighian tubules in the fruit fly drosophila melanogaster has discouraged measurements of the transepithelial electrical resistance. the present study introduces two methods for measuring the transepithelial resistance in isolated d. melanogaster malpighian tubules using conventional microelectrodes and pclamp hardware and software. the first method uses three microelectrodes to measure the specific transepithelial resistance normalized to tubule length or luminal surface are ...201931043456
similar neural pathways control foraging in mosquitoes and worms.female aedes aegypti mosquitoes bite human hosts to obtain a blood meal and, in the process, act as vectors for many disease-causing viruses, including the dengue, chikungunya, yellow fever, and zika viruses. after a complete meal, the female mosquitoes lose attraction to their hosts for several days. new research shows that pharmacological activation of neuropeptide y-like receptor (npylr) signaling elicits host aversion in female mosquitoes. this behavior of mosquitoes shows remarkable similar ...201931040241
resistance to temephos and deltamethrin in aedes aegypti from brazil between 1985 and 2017.aedes aegypti populations in brazil have been subjected to insecticide selection pressures with variable levels and sources since 1967. therefore, the brazilian ministry of health (moh) coordinated the activities of an ae. aegypti insecticide resistance monitoring network (morenaa) from 1999 to 2012.201931038548
using the basic reproduction number to assess the risk of transmission of lumpy skin disease virus by biting insects.in recent years, lumpy skin disease virus (lsdv) has emerged as a major threat to cattle outside africa, where it is endemic. although evidence suggests that lsdv is transmitted by the bites of blood sucking arthropods, few studies have assessed the risk of transmission posed by particular vector species. here this risk is assessed by calculating the basic reproduction number (r0 ) for transmission of lsdv by five species of biting insect: the stable fly, stomoxys calcitrans, the biting midge, c ...201931038286
aedes aegypti. 201931036346
exploring deeper genetic structures: aedes aegypti in brazil.aedes aegypti, being the principal vector of dengue (denv1 to 4), chikungunya and zika viruses, is considered as one of the most important mosquito vectors. in brazil, despite regular vector control programs, ae. aegypti still persists with high urban density in all the states. this study aimed to estimate the intra and inter population genetic diversity and genetic structure among 15 brazilian populations of ae. aegypti based on 12 microsatellite loci. a total of 510 specimens were analyzed com ...201931034798
distribution and relative densities of aedes aegypti in niger.its climate and environmental conditions put niger at particularly high risk of the spread of aedes mosquitoes, which can transmit arboviruses. in september 2016, the republic of niger reported its first outbreak of rift valley fever (rvf) in the northern region of tahoua, near the mali border, particularly in the departments of tchintabaraden, tassara, and abalak. the history of rvf has showed that epidemics and epizootics have a considerable socioeconomic impact in affected countries, such as ...201931031247
aedes aegypti mosquitoes use their legs to sense deet on contact.deet (n, n-diethyl-meta-toluamide) is the most effective and widely used insect repellent, but its mechanism of action is both complex and controversial [1]. deet acts on insect smell [2-6] and taste [7-11], and its olfactory mode of action requires the odorant co-receptor orco [2, 3, 6]. we previously observed that orco mutant female aedes aegypti mosquitoes are strongly attracted to humans even in the presence of deet, but they are rapidly repelled after contacting deet-treated skin [6]. deet ...201931031114
resistance status to deltamethrin, permethrin, and temephos along with preliminary resistance mechanism in aedes aegypti (diptera: culicidae) from punjab, pakistan.the use of insecticides such as deltamethrin, permethrin, and temephos has been a primary tool to manage aedes aegypti (linnaeus) in punjab province, pakistan; however, recent reports of control failure necessitate monitoring insecticide resistance. for this reason, we evaluated 12 field strains of ae. aegypti from punjab for resistance against deltamethrin, permethrin, and temephos along with underlying resistance mechanisms. for deltamethrin, high level of resistance was observed in rawalpindi ...201931028391
association of age, sex, and pyrethroid resistance status on survival and cytochrome p450 gene expression in aedes aegypti (l.).aedes aegypti is a vector of viruses that negatively impact human health. insecticide resistance complicates mosquito control efforts, but understanding the mechanisms of resistance can help to improve management practices. this study examined different factors that could influence the interpretation of toxicity bioassays and gene expression studies in a. aegypti, including sex and age, in the context of resistance to pyrethroids. bioassays using a pyrethroid-resistant strain, puerto rico (pr), ...201931027587
"armed to the teeth": the multiple ways to survive insecticidal and predatory challenges in aedes aegypti larvae.environmental pollutants, such as insecticides, can alter the equilibrium of aquatic ecosystems, particularly those closely located to human occupations. the use of such anthropogenic compounds frequently results in the selection of resistant individuals. however, how the underlying insecticide resistance mechanisms interplay with the abilities of the resistant individuals to cope with other environmental challenges (e.g., predators) has not received adequate attention. here, we evaluated whethe ...201931027585
aedes-chikungunya virus interaction: key role of vector midguts microbiota and its saliva in the host infection.aedes mosquitoes are important vectors for emerging diseases caused by arboviruses, such as chikungunya (chikv). these viruses' main transmitting species are aedes aegypti and ae. albopictus, which are present in tropical and temperate climatic areas all over the globe. knowledge of vector characteristics is fundamentally important to the understanding of virus transmission. only female mosquitoes are able to transmit chikv to the vertebrate host since they are hematophagous. in addition, mosqui ...201931024463
microorganism-based larval diets affect mosquito development, size and nutritional reserves in the yellow fever mosquito aedes aegypti (diptera: culicidae).mosquito larvae feed on organic detritus from the environment, particularly microorganisms comprising bacteria, protozoa, and algae as well as crustaceans, plant debris, and insect exuviae. little attention has been paid to nutritional studies in aedes aegypti larvae.201931024326
cell death induced by α-terthienyl via reactive oxygen species-mediated mitochondrial dysfunction and oxidative stress in the midgut of aedes aegypti larvae.α-terthienyl (α-t) is a photosensitizer that produces many reactive oxygen species (ros) under ultraviolet light. here, we aimed to evaluate the oxidation mechanism of the 25%, 50%, and 75% lethal concentrations in aedes aegypti larvae; the lethal concentration of α-t was used as the test value. the effects on mitochondria, oxidative stress, and cell death patterns caused by ros were evaluated. the results showed that α-t mainly produced large amounts of ros in the midgut of larvae. moreover, mi ...201931022448
life on the margin: rainwater tanks facilitate overwintering of the dengue vector, aedes aegypti, in a sub-tropical climate.a key determinant of insect persistence in marginal habitats is the ability to tolerate environmental extremes such as temperature. aedes aegypti is highly invasive and little is known about the physiological sensitivity of the species to fluctuating temperature regimes at the lower critical threshold for development. a temperature that may limit the establishment and persistence of the species in sub-optimal regions. daily winter temperatures were measured in common australian larval habitats, ...201931022231
application of a targeted-enrichment methodology for full-genome sequencing of dengue 1-4, chikungunya and zika viruses directly from patient samples.the frequency of epidemics caused by dengue viruses 1-4, zika virus and chikungunya viruses have been on an upward trend in recent years driven primarily by uncontrolled urbanization, mobility of human populations and geographical spread of their shared vectors, aedes aegypti and aedes albopictus. infections by these viruses present with similar clinical manifestations making them challenging to diagnose; this is especially difficult in regions of the world hyperendemic for these viruses. in thi ...201931022183
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