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the efficiency of a new automated mosquito larval counter and its impact on larval survival.to achieve consistent and standardized rearing for mosquito immature stages, it is crucial to control the initial number of larvae present in each larval tray. in addition, maintaining an optimal and synchronized development rate of larvae is essential to maximize the pupal production and optimize male sorting in a mass-rearing setting. manual counting is labor intensive, time consuming and error prone. therefore, this study aimed to investigate the use of a customized automated counter for the ...201931092868
infection of aedes albopictus mosquito c6/36 cells with the wmelpop strain of wolbachia modulates dengue virus-induced host cellular transcripts and induces critical sequence alterations in the dengue viral genome.dengue virus (denv) causes frequent epidemics infecting ∼390 million people annually in over 100 countries. there are no approved vaccines or antiviral drugs for treatment of infected patients. however, there is a novel approach to control denv transmission by the mosquito vectors, aedes aegypti and aedes albopictus, using the wolbachia symbiont. the wmelpop strain of wolbachia suppresses denv transmission and shortens the mosquito life span. however, the underlying mechanism is poorly understoo ...201931092581
microencapsulation of citrus grandis peel oil using interfacial precipitation chemistry technique for repellent application.essential oil of citrus family plant is known to have repellent effect against mosquito. unfortunately, due to its high volatility effect, its repellency effect was compromised. the incorporation of essential oil in a microencapsulation formulation has been shown to help improve the stability and potency of the repellent. in this study, citrus grandis peel oil (cgpo) was encapsulated by using the interfacial precipitation chemistry technique. the microencapsulated cgpo was then formulated into l ...201931089355
larvicidal, adulticidal, and oviposition-deterrent activity of piper betle l. essential oil to aedes aegypti.aedes aegypti is a primary vector of many arthropod-borne diseases. one of the diseases, dengue fever, is an endemic disease in indonesia causing high mortalities for decades. there are no preventive and specific treatments for dengue so far. therefore, prevention of this disease largely depends on the mosquito control. since resistance to chemical insecticides occurred worldwide, the study on alternate and new mosquito insecticides are mandatory. this study aimed to demonstrate the effect of es ...201931089304
efficacy of the in2care® auto-dissemination device for reducing dengue transmission: study protocol for a parallel, two-armed cluster randomised trial in the philippines.mosquito-borne viruses are imposing an ever increasing health burden worldwide. in addition to the recent zika and chikungunya virus epidemics, dengue viruses have become the fastest growing problem with a 40-fold increase in the number of reported cases over the past five decades. current mosquito control techniques involving larval source reduction, larviciding, and space spray of adulticides are costly, laborious, and of debatable efficacy. there remains an urgent need for the development of ...201931088515
glycosaminoglycan compositional analysis of relevant tissues in zika virus pathogenesis and in vitro evaluation of heparin as an antiviral against zika virus infection.zika virus (zikv) is an enveloped rna virus from the flavivirus family that can cause fetal neural abnormalities in pregnant women. previously, we established that zikv-ep (envelope protein) binds to human placental chondroitin sulfate (cs), suggesting that cs may be a potential host cell surface receptor in zikv pathogenesis. in this study, we further characterized the gag disaccharide composition of other biological tissues (i.e., mosquitoes, fetal brain cells, and eye tissues) in zikv pathoge ...201930698412
presence of the point mutations val1016gly in the voltage-gated sodium channel detected in a single mosquito from panama.in panama, arboviroses such as dengue fever, and more recently chikungunya fever and zika disease, are transmitted by aedes aegypti and aedes albopictus. their control is based on the elimination of breeding sites and fogging with pyrethroid insecticides. however, one of the significant issues derived from the prolonged use of pyrethroid insecticide is the development of resistance mechanisms, such as knockdown resistance or kdr. the objective of this study was to evaluate the presence of kdr mu ...201930691518
identification of potential inhibitors from pyriproxyfen with insecticidal activity by virtual screening.aedes aegypti is the main vector of dengue fever transmission, yellow fever, zika, and chikungunya in tropical and subtropical regions and it is considered to cause health risks to millions of people in the world. in this study, we search to obtain new molecules with insecticidal potential against ae. aegypti via virtual screening. pyriproxyfen was chosen as a template compound to search molecules in the database zinc_natural_stock (znst) with structural similarity using rocs (rapid overlay of c ...201930691028
factors affecting short-range host-seeking for the yellow fever mosquito (diptera: culicidae).understanding short-range cues (e.g., host odorants, heat, moisture) of host-seeking female aedes aegypti l. is very important for attempts to reduce mosquito bites, to complement current control strategies, and to develop potential spatial repellents. we investigated behavior under semi-field conditions utilizing a new portable uni-port taxis box with a caged chicken host. the combined influences of airflow regimes (0, 1.5, 3, 5, and 6 m/s), distance from host odor (10, 50, 100 cm), host-odor c ...201930689913
predation on eggs of aedes aegypti (diptera: culicidae): temporal dynamics and identification of potential predators during the winter season in a temperate region.in temperate regions, the populations of aedes aegypti (l.) remain in the egg stage during the cold season. the ability of these eggs to survive until the next favorable season is affected by several mortality factors, including the action of predators. in the present study, we analyzed the temporal dynamics of the loss of eggs and identified predators of ae. aegypti eggs along the unfavorable season in a temperate region of argentina. to this end, eggs were exposed in field conditions in pitfal ...201930689912
microbial exopolymer-capped selenium nanowires - towards new antibacterial, antibiofilm and arbovirus vector larvicides?arboviral diseases and microbial pathogens resistant to commercially available drugs are on the rise. herein, a facile microbial-based approach was developed to synthesize selenium nanowires (se nws) using microbial exopolymer (mep) extracted from the bacillus licheniformis (probiotic bacteria). mep-se nws were characterized using uv-visible, xrd, ftir, hr-tem, fe-sem and edx. an uv-visible peak was detected at 330 nm while xrd spectrum data pointed out the crystalline nature of mep-se nws. ftir ...201930685584
complete nmr chemical shift assignments of odorant binding protein 22 from the yellow fever mosquito, aedes aegypti, bound to arachidonic acid.aedes aegypti mosquitoes are the vector for transmission of dengue, zika and chikungunya viruses. these mosquitos feed exclusively on human hosts for a blood meal. previous studies have established that dengue virus infection of the mosquito results in increased expression of the odorant binding proteins 22 and 10 within the mosquito salivary gland and silencing of these genes dramatically reduces blood-feeding behaviors. odorant binding proteins are implicated in modulating the chemosensory per ...201930684234
toxicity and possible mechanisms of action of honokiol from magnolia denudata seeds against four mosquito species.this study was performed to determine the toxicity and possible mechanism of the larvicidal action of honokiol, extracted from magnolia denudata seeds, and its 10 related compounds against third-instar larvae of insecticide-susceptible culex pipiens pallens, aedes aegypti, and aedes albopictus and anopheles sinensis resistant to deltamethrin and temephos. honokiol (lc50, 6.13-7.37 mg/l) was highly effective against larvae of all of the four mosquito species, although the toxicity of the compound ...201930674912
chemical composition, aedes mosquito larvicidal activity, and repellent activity against triatoma rubrofasciata of severinia monophylla leaf essential oil.aedes aegypti and ae. albopictus are key vectors in the spread of arboviruses such as dengue, chikungunya, yellow fever, and zika. triatoma rubrofasciata is an "assassin bug" whose populations and association with humans have dramatically increased and may represent a serious health concern. control of insect vectors is a logical course of action to prevent the spread of these insect-borne infections. this work presents the leaf essential oil composition, mosquito larvicidal activities, and inse ...201930671730
comparative efficacy of eucalyptus globulus (labill) hydrodistilled essential oil and temephos as mosquito larvicide.in this study eucalyptus globulus essential oil was tested on major mosquito species and toxicity was compared with temephos. mortality was calculated after 24 h and 48 h post treatment. in 24 h treatment essential oil show strong larvicidal activity with lc50 and lc90 values were 30.198ppm, 103.389ppm for anopheles stephensi, 13.578ppm, 106.755ppm for aedes aegypti; and, 7.469ppm, 32.454ppm for culex quinquefasciatus and 48 h post treatment lc50 and lc90 values were, 12.576, 49.380ppm for anoph ...201930623679
a novel entomological index, aedes aegypti breeding percentage, reveals the geographical spread of the dengue vector in singapore and serves as a spatial risk indicator for dengue.aedes aegypti is an efficient primary vector of dengue, and has a heterogeneous distribution in singapore. aedes albopictus, a poor vector of dengue, is native and ubiquitous on the island. though dengue risk follows the dispersal of ae. aegypti, the spatial distribution of the vector is often poorly characterized. here, based on the ubiquitous presence of ae. albopictus, we developed a novel entomological index, ae. aegypti breeding percentage (bp), to demonstrate the expansion of ae. aegypti i ...201930621762
matching the genetics of released and local aedes aegypti populations is critical to assure wolbachia invasion.traditional vector control approaches such as source reduction and insecticide spraying have limited effect on reducing aedes aegypti population. the endosymbiont wolbachia is pointed as a promising tool to mitigate arbovirus transmission and has been deployed worldwide. models predict a rapid increase on the frequency of wolbachia-positive ae. aegypti mosquitoes in local settings, supported by cytoplasmic incompatibility (ci) and high maternal transmission rate associated with the wmelbr strain ...201930620733
identification and characterization of a mosquito-specific eggshell organizing factor in aedes aegypti mosquitoes.mosquito-borne diseases are responsible for several million human deaths annually around the world. one approach to controlling mosquito populations is to disrupt molecular processes or antagonize novel metabolic targets required for the production of viable eggs. to this end, we focused our efforts on identifying proteins required for completion of embryonic development that are mosquito selective and represent potential targets for vector control. we performed bioinformatic analyses to identif ...201930620728
vertical transmission of zika virus in culex quinquefasciatus say and aedes aegypti (l.) mosquitoes.several mosquito species have been described as vectors for the zika virus (zikv), such as those in the aedes, anopheles, mansonia and culex genera. our previous survey studies were found the zikv rna positive in both male, female and larvae of culex quinquefasciatus say and aedes aegypti (l.) mosquitoes collected from active zikv infected patients' homes in thailand. therefore, the aims of this study were to investigate whether zikv could be vertically transmitted in cx. quinquefasciatus, ae. a ...201930918310
evaluation of insecticide resistance in aedes aegypti populations connected by roads and rivers: the case of tocantins state in brazil.the longstanding application of insecticides for vector control without periodic monitoring of the population response to these chemicals can directly drive the selection of resistant populations of vector mosquitoes. tocantins is the newest state of the brazilian federation. despite a historically high number of dengue cases, studies and monitoring data concerning insecticide resistance in the state are lacking.201930916115
understanding the mechanism of chikungunya virus vector competence in three species of mosquitoes.chikungunya virus (chikv) is primarily transmitted by aedes spp. mosquitoes. the present study investigated vector competence for chikv in aedes aegypti and aedes albopictus mosquitoes found in madurai, south india. the role of receptor proteins on midguts contributing to permissiveness of chikv to aedes spp. mosquitoes was also undertaken. mosquitoes were orally infected with chikv drde-06. infection of midguts and dissemination to heads was confirmed by immunofluorescence assay at different ti ...201930913314
mosquitoes on a diet reduce those pesky bites.targeting the internal regulation of mosquito's human-seeking capacity provides a novel means for vector control. mosquitoes bite us to obtain blood in order to develop their eggs. professor vosshall and colleagues (cell 2019;176:687-701) have exploited the modulatory pathway to control this hunger for blood by designing drugs based on human diet-suppression pharmaceuticals.201930910491
high-throughput screening method for evaluating spatial repellency and vapour toxicity to mosquitoes.spatial repellents are an essential tool for personal protection against mosquitoes that bite and transmit disease pathogens to humans. current repellent screening methods, such as olfactometers and alternative choice tests, are complex systems that require a relatively large quantity of compound (mg). the present study validates a high-throughput spatial repellent screening method using a glass tube that has the ends covered with netting, in addition to treated filters and plastic end caps. the ...201930907445
mitochondrial affectation, dna damage and ache inhibition induced by salvia officinalis essential oil on aedes aegypti larvae.the aim of this research study was to understand the mechanism of action of salvia officinalis (lamiaceae) essential oil (eo) on aedes aegypti larvae. we evaluated the effect on dna damage, acetylcholinesterase (ache) inhibition and mitochondrial enzymatic alterations. the major components were analyzed in silico using osiris and molispiration free software. aedes aegypti dna was extracted from mosquito larvae between third (l3) and fourth (l4) instars to determine the dna fragmentation or degra ...201930905844
phytochemical composition and larvicidal activity of essential oils from herbal plants.the essential oils (eos) of plectranthus amboinicus showed the highest larvicidal activity among four herbal plants studied and β-caryophyllene might be the major component responsible for its differential toxicity to the larvae of culex quinquefasciatus and aedes aegypti. mosquitoes act as vectors for many life-threatening diseases, including malaria, dengue fever, and zika virus infection. management of mosquitoes mainly relies on synthetic insecticides, which usually result in the rapid devel ...201930904944
publisher correction: past and future spread of the arbovirus vectors aedes aegypti and aedes albopictus.in the version of this article originally published, the affiliation for author catherine linard was incorrectly stated as '6department of infectious disease epidemiology, london school of hygiene and tropical medicine, london, uk'. the correct affiliation is '9spatial epidemiology lab (spell), universite libre de bruxelles, brussels, belgium'. the affiliation for author hongjie yu was also incorrectly stated as '11department of statistics, harvard university, cambridge, ma, usa'. the correct af ...201930903094
aedes mosquitoes acquire and transmit zika virus by breeding in contaminated aquatic environments.zika virus (zikv) is a mosquito-borne flavivirus that predominantly circulates between humans and aedes mosquitoes. clinical studies have shown that zika viruria in patients persists for an extended period, and results in infectious virions being excreted. here, we demonstrate that aedes mosquitoes are permissive to zikv infection when breeding in urine or sewage containing low concentrations of zikv. mosquito larvae and pupae, including from field aedes aegypti can acquire zikv from contaminate ...201930902991
self-assembly of chlorin-e6 on γ-fe2o3 nanoparticles: application for larvicidal activity against aedes aegypti.aedes aegypti mosquitos are widespread vectors of several diseases and their control is of primary importance for biological and environmental reasons, and novel safe insecticides are highly desirable. an eco-friendly photosensitizing magnetic nanocarrier with larvicidal effects on aedes aegypti was proposed. the innovative core-shell hybrid nanomaterial was synthesized by combining peculiar magnetic nanoparticles (called surface active maghemite nanoparticles - samns, the core) and chlorin-e6 a ...201930897400
male accessory gland molecules inhibit harmonic convergence in the mosquito aedes aegypti.aedes aegypti mosquitoes transmit pathogens such as yellow fever, dengue, zika, and chikungunya viruses to millions of human hosts annually [1]. as such, understanding ae. aegypti courtship and mating biology could prove crucial to the success of disease control efforts that target reproduction. potentially to communicate reproductive fitness [2,3], mosquito males and females harmonize their flight tones prior to mating in a behavior known as harmonic convergence (hc) [4]. furthermore, after mat ...201930889386
environmental concentrations of antibiotics may diminish wolbachia infections in aedes aegypti (diptera: culicidae).wolbachia-infected aedes aegypti (l.) mosquitoes for control of dengue transmission are being released experimentally in tropical regions of australia, south-east asia, and south america. to become established, the wolbachia hertig (rickettsiales: rickettsiaceae) strains used must induce expression of cytoplasmic incompatibility (ci) in matings between infected males and uninfected females so that infected females have a reproductive advantage, which will drive the infection through field popula ...201930889242
climate change may enable aedes aegypti infestation in major european cities by 2100.climate change allows aedes aegypti to infest new areas. consequently, it enables the arboviruses the mosquito transmits -- e.g., dengue, chikungunya, zika and yellow fever - to emerge in previously uninfected areas. an example is the portuguese island of madeira during 2012-13.201930884421
update on the geographical distribution and prevalence of aedes aegypti and aedes albopictus (diptera: culicidae), two major arbovirus vectors in cameroon.arboviral diseases including dengue are increasingly spreading in the tropical/subtropical world including africa. updated knowledge on the distribution and abundance of the major vectors aedes aegypti and aedes albopictus constitutes crucial surveillance action to prepare african countries such as cameroon for potential arbovirus outbreaks. here, we present a nationwide survey in cameroon to assess the current geographical distribution and prevalence of both vectors including a genetic diversit ...201930883552
effects of prenatal exposure to temephos on behavior and social interaction.the neurodevelopment period is susceptible to alterations by genetic and environmental factors, such as the exposure to organophosphates (op). the op is neurotoxic and has been associated with neurological diseases pathophysiology. the op temephos is widely used against aedes aegypti in brazil's public health programs.201930880993
heterologous expression of aedes aegypti cation chloride cotransporter 2 (aeccc2) in xenopus laevis oocytes induces an enigmatic na⁺/li⁺ conductance.the yellow fever mosquito aedes aegypti possesses three genes encoding putative na⁺-coupled cation chloride cotransporters (cccs): aenkcc1, aeccc2, and aeccc3. to date, none of the aecccs have been functionally characterized. here we expressed aeccc2 heterologously in xenopus oocytes and measured the uptake of li⁺ (a tracer for na⁺) and rb⁺ (a tracer for k⁺). compared to control (h₂o-injected) oocytes, the aeccc2-expressing oocytes exhibited significantly greater uptake of li⁺, but not rb⁺. howe ...201930875796
biochemical and functional characterization of glycoside hydrolase family 16 genes in aedes aegypti larvae: identification of the major digestive β-1,3-glucanase.insect β-1,3-glucanases belong to glycoside hydrolase family 16 (ghf16) and are involved in digestion of detritus and plant hemicellulose. in this work, we investigated the role of ghf16 genes in aedes aegypti larvae, due to their detritivore diet. aedes aegypti genome has six genes belonging to ghf16 (aae gh16.1 - aae gh16.6), containing two to six exons. sequence analysis suggests that five of these ghf16 sequences (aae gh16.1, 2, 3, 5, and 6) contain the conserved catalytic residues of this f ...201930873040
development and validation of reverse transcription loop-mediated isothermal amplification (rt-lamp) for rapid detection of zikv in mosquito samples from brazil.the rapid spread of zika virus (zikv) represents a global public health problem, especially in areas that harbor several mosquito species responsible for virus transmission, such as brazil. in these areas, improvement in mosquito control needs to be a top priority, but mosquito viral surveillance occurs inefficiently in zikv-endemic countries. quantitative reverse transcription pcr (qrt-pcr) is the gold standard for molecular diagnostic of zikv in both human and mosquito samples. however, the te ...201930872672
the pharmacological and functional characterization of the serotonergic system in anopheles gambiae and aedes aegypti: influences on flight and blood-feeding behavior.aedes aegypti and anopheles gambiae harbor the causative agents of diseases such as dengue fever and malaria, afflicting human morbidity and mortality worldwide. given the worldwide emergence of resistance to insecticides, the current mainstay for vector control, identification of alternative modes of action for future insecticides is paramount. the serotonergic (5-ht) system has been documented to impact physiological mechanisms involved in disease transmission, suggesting its potential as a ne ...201930872615
protein expression in female salivary glands of pyrethroid-susceptible and resistant strains of aedes aegypti mosquitoes.a group of insecticides called pyrethroids has been used extensively worldwide and development of pyrethroid resistance within mosquito populations, especially in aedes aegypti, has rapidly spread through populations. in this study, sds-page, 2-de coupled with nanolc-ms, and bioinformatics were used to analyze the female salivary gland proteins of pyrethroid-susceptible (pmd) and pyrethroid-resistant (pmd-r and upk-r) strains of ae. aegypti mosquitoes for the first time.201930871604
evidence for the natural occurrence of wolbachia in aedes aegypti mosquitoes.wolbachia, a gram-negative bacterium, is widely known to be present in arthropods and nematodes. of late, great impetus is given to employ this intracellular bacterium, as an alternative to conventional biocontrol agents for the control of mosquitoes because of its inherent ability to induce sperm-egg incompatibility, feminisation etc. by employing molecular tools, we have shown the presence of wolbachia from aedes aegypti mosquito population collected from coimbatore, india by pcr amplifying th ...201930869785
corrigendum to "larvicidal potential of five selected dragonfly nymphs in sri lanka over aedes aegypti (linnaeus) larvae under laboratory settings".[this corrects the article doi: 10.1155/2018/8759459.].201930868069
first dengue virus seroprevalence study on madeira island after the 2012 outbreak indicates unreported dengue circulation.in 2012, the first dengue virus outbreak was reported on the portuguese island of madeira with 1080 confirmed cases. dengue virus of serotype 1 (denv-1), probably imported from venezuela, caused this outbreak with autochthonous transmission by invasive aedes aegypti mosquitoes.201930867031
genome-wide divergence among invasive populations of aedes aegypti in california.in the summer of 2013, aedes aegypti linnaeus was first detected in three cities in central california (clovis, madera and menlo park). it has now been detected in multiple locations in central and southern ca as far south as san diego and imperial counties. a number of published reports suggest that ca populations have been established from multiple independent introductions.201930866822
naturally infected aedes aegypti collected during a zika virus outbreak have viral titres consistent with transmission. 201930866777
costs of an aedes aegypti vector control program. 201930864616
larvicidal activity of selected essential oil in synergized combinations against aedes aegypti.this study was conducted to attain an alternate plant essential oil (eo) based mosquito larvicide against the dengue vector aedes aegypti. here three plants were selected based on their local availability, safety and insecticidal properties. eos were extracted from syzygium aromaticum (myrtaceae) flower buds, fruits of illicium verum (schisandraceae) and trachyspermum ammi (apiaceae) by hydro-distillation and tested against ae. aegypti larvae individually and in combinations to find synergistic ...201930861442
global outbreaks and origins of a chikungunya virus variant carrying mutations which may increase fitness for aedes aegypti: revelations from the 2016 mandera, kenya outbreak.in 2016, a chikungunya virus (chikv) outbreak was reported in mandera, kenya. this was the first major chikv outbreak in the country since the global reemergence of this virus in kenya in 2004. we collected samples and sequenced viral genomes from this outbreak. all kenyan genomes contained two mutations, e1:k211e and e2:v264a, recently reported to have an association with increased infectivity, dissemination, and transmission in the aedes aegypti vector. phylogeographic inference of temporal an ...201930860010
aedes aegypti agbr1 antibodies modulate early zika virus infection of mice.a recent epidemic of zika virus in the americas, affecting well over a million people, caused substantial mortality and morbidity, including guillain-barre syndrome, microcephaly and other fetal developmental defects1,2. preventive and therapeutic measures that specifically target the virus are not readily available. the transmission of zika virus is predominantly mosquito-borne, and aedes aegypti mosquitoes serve as a key vector for zika virus3. here, to identify salivary factors that modulate ...201930858571
modeling the impact of sterile males on an aedes aegypti population with optimal control.we use partial differential equations to describe the dynamics of an aedes aegypti mosquito population on an island, and the effects of a sterile male release. the model includes mosquito movement and an allee effect to capture extinction events. we apply optimal control theory to identify the release strategy that eliminates the mosquitoes most rapidly, conditional on a limited availability of sterile males. the optimal solution for a single location is to initially release a substantial number ...201930857942
laboratory evaluation of larvicidal and oviposition deterrent properties of edible plant oils for potential management of aedes aegypti (diptera: culicidae) in drinking water containers.the yellow fever mosquito, aedes aegypti (diptera: culicidae) transmits several devastating arboviruses, including dengue, chikungunya, and zika virus, making development of inexpensive and eco-friendly strategies for its control an urgent priority. we evaluated the lethality of 13 commonly used plant-derived edible oils against late-third instar ae. aegypti and then tested the three most lethal oils for stage-specific differences in lethality. we also examined the effects of the most lethal (he ...201930855084
udp-glycosyltransferase genes and their association and mutations associated with pyrethroid resistance in anopheles sinensis (diptera: culicidae).udp-glycosyltransferase (ugt) is an important biotransformation superfamily of enzymes. they catalyze the transfer of glycosyl residues from activated nucleotide sugars to acceptor hydrophobic molecules, and function in several physiological processes, including detoxification, olfaction, cuticle formation, pigmentation. the diversity, classification, scaffold location, characteristics, phylogenetics, and evolution of the superfamily of genes at whole genome level, and their association and muta ...201930845961
molecular epidemiology and genetic diversity of zika virus from field-caught mosquitoes in various regions of thailand.zika virus (zikv) infection is an emerging and re-emerging arbovirus disease that is transmitted to humans through the bite of infected mosquitoes. zikv infections were first described in thailand in 1954 from the sera of indigenous residents and several travelers returning from thailand in 2014. however, reported cases in thailand have been increasing since 2015 and 2016, and epidemiological information about the vectors of zikv is unclear. we investigated the molecular epidemiology and genetic ...201930845707
updated methods for the production of toxorhynchites rutilus septentrionalis (diptera, culicidae) for use as biocontrol agent against container breeding pest mosquitoes in harris county, texas.the complex biologies of pestiferous mosquito species complicate the development of a single, across the board effective control measure. the use of traditional biological control through predators, parasitoids, and microbes, though part of a multimodal, integrated pest management approach, is scant in current standard mosquito control operations. at this time, traditional, whole organism mosquito biocontrol agents are not commercially available, and if their integration into a release program i ...201930843583
urban and semi-urban mosquitoes of mexico city: a risk for endemic mosquito-borne disease transmission.since past century, vector-borne diseases have been a major public health concern in several states of mexico. however, mexico city continues to be free of endemic mosquito-borne viral diseases. the city is the most important politic and economic state of mexico and one of the most important city of latin america. its subtropical highland climate and high elevation (2240 masl) had historically made the occurrence of aedes species unlikely. however, the presence of other potential disease vectors ...201930840661
activity of additives and their effect in formulations of metarhizium anisopliae s.l. ip 46 against aedes aegypti adults and on post mortem conidiogenesis.oil formulations of entomopathogenic fungi have interest for biological mosquito control.201930836061
epidemiological and clinical characteristics of dengue virus outbreaks in two regions of china, 2014 - 2015.dengue virus (denv), a single-stranded rna virus and flaviviridae family member, is transmitted by aedes aegypti and aedes albopictus mosquitoes. denv causes dengue fever, which may progress to severe dengue. hospital-based surveillance was performed in two chinese regions, guangzhou and xishuangbanna, during the dengue epidemics in 2014 and 2015, respectively. acute-phase serum was obtained from 133 patients with suspected dengue infections during the peak season for dengue cases. viremia level ...201930835769
transcriptome sequencing and analysis of changes associated with insecticide resistance in the dengue mosquito (aedes aegypti) in vietnam.the mosquito aedes aegypti is a transmission vector for dangerous epidemic diseases in humans. insecticides have been used as the most general vector control method in the world. however, ae. aegypti have developed many resistant mechanisms such as reduced neuronal sensitivity to insecticides (target-site resistance), enhanced insecticide metabolism (metabolic resistance), altered transport, sequestration, and other mechanisms. it has become a major problem for vector control programs. transcrip ...201930834881
past and future spread of the arbovirus vectors aedes aegypti and aedes albopictus.the global population at risk from mosquito-borne diseases-including dengue, yellow fever, chikungunya and zika-is expanding in concert with changes in the distribution of two key vectors: aedes aegypti and aedes albopictus. the distribution of these species is largely driven by both human movement and the presence of suitable climate. using statistical mapping techniques, we show that human movement patterns explain the spread of both species in europe and the united states following their intr ...201930833735
discovery and characterization of 2-nitro-5-(4-(phenylsulfonyl)piperazin-1-yl)- n-(pyridin-4-ylmethyl)anilines as novel inhibitors of the aedes aegypti kir1 ( aekir1) channel.mosquito-borne arboviral diseases such as zika, dengue fever, and chikungunya are transmitted to humans by infected adult female aedes aegypti mosquitoes and affect a large portion of the world's population. the kir1 channel in ae. aegypti ( aekir1) is an important ion channel in the functioning of mosquito malpighian (renal) tubules and one that can be manipulated in order to disrupt excretory functions in mosquitoes. we have previously reported the discovery of various scaffolds that are activ ...201930832472
characterization, diversity, and structure-activity relationship study of lipoamino acids from pantoea sp. and synthetic analogues.a biological evaluation of a library of extracts from entomopathogen strains showed that pantoea sp. extract has significant antimicrobial and insecticidal activities. three hydroxyacyl-phenylalanine derivatives were isolated from this strain. their structures were elucidated by a comprehensive analysis of their nmr and ms spectroscopic data. the antimicrobial and insecticidal potencies of these compounds were evaluated, and compound 3 showed 67% mortality against aedes aegypti larvae at a conce ...201930832353
determination by chromatography and cytotoxotoxic and oxidative effects of pyriproxyfen and pyridalyl.pyriproxyfen (ppf) is a larvicide, used to combat the proliferation of aedes aegypti larvae. the objective of this study was to analyze the compounds of pyriproxyfen and pyridalyl (pyl) in a commercial larvicide to analyze the cytotoxic and oxidative effects of ppf and pyl. the toxic potential of ppf and pyl were assessed based on lethal concentration (lc50) in artemia salina, cytotoxicity based on the mitotic index and the chromosomal alterations in allium cepa and the oxidative damage in sacch ...201930831490
effects of methylmercury on mosquito oviposition behavior: maladaptive response to non-toxic exposure.animals can modulate their own exposure to environmental contaminants through behavioral plasticity such as diet and habitat choice. however, it remains unclear if behavior also has cascading effects on contaminant exposure across multiple generations. in insects, oviposition site selection is an important behavior females can use to modify offspring contaminant exposure risk. in this study, we use the yellow fever mosquito, aedes aegypti, to test how methylmercury (mehg) affects oviposition sit ...201930831364
protocols for testing the toxicity of novel insecticidal chemistries to mosquitoes.new classes of insecticides with novel modes of action are needed to control insecticide resistant populations of mosquitoes that transmit diseases such as zika, dengue and malaria. assays for rapid, high-throughput analyses of unformulated novel chemistries against mosquito larvae and adults are presented. we describe protocols for single point-dose and dose response assays to evaluate the toxicity of small molecule chemistries to the aedes aegypti vector of zika, dengue and yellow fever, the m ...201930829331
a comprehensive assessment of inbreeding and laboratory adaptation in aedes aegypti mosquitoes.modified aedes aegypti mosquitoes reared in laboratories are being released around the world to control wild mosquito populations and the diseases they transmit. several efforts have failed due to poor competitiveness of the released mosquitoes. we hypothesized that colonized mosquito populations could suffer from inbreeding depression and adapt to laboratory conditions, reducing their performance in the field. we established replicate populations of ae. aegypti mosquitoes collected from queensl ...201930828375
the first outbreak of dengue fever in greater darfur, western sudan.dengue virus (denv) is an arthropod-borne virus (arbovirus) transmitted by the aedes mosquitoes, mainly aedes aegypti. dengue fever is a rapidly growing disease with expanding geographical distribution worldwide. we investigated a high number of non-malaria febrile cases reported to health clinics in refugee camps in the five states of darfur between august 2015 and march 2016. the clinical presentation of cases and case definition criteria suggested involvement of one or more arboviral hemorrha ...201930823624
trap location and premises condition influences on aedes aegypti (diptera: culicidae) catches using biogents sentinel traps during a 'rear and release' program: implications for designing surveillance programs.as the incidence of arboviral diseases such as dengue, zika, chikungunya, and yellow fever increases globally, controlling their primary vector, aedes aegypti (l.) (diptera: culicidae), is of greater importance than ever before. mosquito control programs rely heavily on effective adult surveillance to ensure methodological efficacy. the biogents sentinel (bgs) trap is the gold standard for surveilling adult aedes mosquitoes and is commonly deployed worldwide, including during modern 'rear and re ...201930817823
efficacy of novel indoor residual spraying methods targeting pyrethroid-resistant aedes aegypti within experimental houses.challenges in maintaining high effectiveness of classic vector control in urban areas has renewed the interest in indoor residual spraying (irs) as a promising approach for aedes-borne disease prevention. while irs has many benefits, application time and intrusive indoor applications make its scalability in urban areas difficult. modifying irs to account for ae. aegypti resting behavior, named targeted irs (tirs, spraying walls below 1.5 m and under furniture) can reduce application time; howeve ...201930817759
socio-ecological factors associated with dengue risk and aedes aegypti presence in the galápagos islands, ecuador.dengue fever is an emerging infectious disease in the galápagos islands of ecuador, with the first cases reported in 2002 and subsequent periodic outbreaks. we report results of a 2014 pilot study conducted in puerto ayora (pa) on santa cruz island, and puerto baquerizo moreno (pb) on san cristobal island. to assess the socio-ecological risk factors associated with dengue and mosquito vector presence at the household level, we conducted 100 household surveys (50 on each island) in neighborhoods ...201930813558
assessment of the effectiveness of bg-sentinel traps baited with co2 and bg-lure for the surveillance of vector mosquitoes in miami-dade county, florida.vector-borne diseases are an increasing issue to public health, endangering billions of people worldwide. controlling vector mosquitoes is widely accepted as the most effective way to prevent vector-borne disease outbreaks. mosquito surveillance is critical for the development of control strategies under the integrated vector management framework. we hypothesize that the effectiveness and reliability of using bg-sentinel traps for the surveillance strongly depend on the bait used to attract mosq ...201930794670
widespread insecticide resistance in aedes aegypti l. from new mexico, u.s.a.aedes aegypti mosquitoes are vectors of a variety of emerging viral pathogens, including yellow fever, dengue, chikungunya, and zika virus. this species has established endemic populations in all cities across southern new mexico sampled to date. presently, control of aedes-borne viruses relies on deployment of insecticides to suppress mosquito populations, but the evolution of insecticide resistance threatens the success of vector control programs. while insecticide resistance is quite common i ...201930794644
high frequencies of f1534c and v1016i kdr mutations and association with pyrethroid resistance in aedes aegypti from somgandé (ouagadougou), burkina faso.resistance to pyrethroid insecticides involving kdr mutations is widespread in aedes aegypti (l.) (diptera: culicidae) and potentially could impact control efforts in endemic countries. dengue cases had been sporadic in burkina faso for over a decade prior to the 2016-2017 outbreak that resulted in 15,074 suspected cases and 36 deaths, mainly in ouagadougou. these outbreaks highlighted the lack of information on numerous aspects of the biology, behaviour and insecticide status of local dengue ve ...201930787670
experimental study on the action of larvicides in aedes aegypti populations collected in the brazilian municipality of itabuna, bahia, under simulated field conditions.to evaluate, under simulated field conditions, the efficacy of pyriproxyfen (juvenile hormone), novaluron (chitin inhibitor) and spinosad (biolarvicide) in controlling aedes aegypti.201930785572
entomological and virological surveillance for dengue virus in churches in merida, mexico.this study was designed to assess whether churches in endemic dengue districts in merida, mexico provide suitable breeding habitats for mosquitoes and are potential sites for dengue virus (denv) transmission. churches were inspected for immature and adult mosquitoes once every week from november 2015 to october 2016. a total of 10,997 immatures of five species were collected. the most abundant species were aedes aegypti (6,051) and culex quinquefasciatus (3,018). the most common source of immatu ...201930785563
origin and expansion of the mosquito aedes aegypti in madeira island (portugal).historically known as the yellow fever mosquito, aedes aegypti invaded madeira island in 2005 and was the vector of the island's first dengue outbreak in 2012. we have studied genetic variation at 16 microsatellites and two mitochondrial dna genes in temporal samples of madeira island, in order to assess the origin of the invasion and the population structure of this mosquito vector. our results indicated at least two independent colonization events occurred on the island, both having a south am ...201930783149
optimization of irradiation dose to aedes aegypti and ae. albopictus in a sterile insect technique program.the sterile insect technique (sit) may offer a means to control the transmission of mosquito borne diseases. sit involves the release of male insects that have been sterilized by exposure to ionizing radiation. we determined the effects of different doses of radiation on the survival and reproductive capacity of local strains of aedes aegypti and ae. albopictus in southern mexico. the survival of irradiated pupae was invariably greater than 90% and did not differ significantly in either sex for ...201930779779
larvicidal and ovicidal activities of artocarpus blancoi extracts against aedes aegypti.dengue control may be done by targeting its vector. in this study, we used artocarpus blancoi (elm.) merr. (moraceae) leaves, an endemic hematophagous insect repellent as a larvicide and ovicide.201930776315
natural vertical infection by dengue virus serotype 4, zika virus and mayaro virus in aedes (stegomyia) aegypti and aedes (stegomyia) albopictus.vertical transmission to progeny ensures the maintenance of arboviruses in their natural vectors. this mechanism is largely reported for dengue virus (denv) and yellow fever virus (yfv). few studies have addressed this mechanism for zika virus (zikv), mayaro virus (mayv) and other arboviruses. the present study investigated the natural infection rate by arboviruses in 4490 aedes (stegomyia) aegypti and 296 aedes (stegomyia) albopictus (diptera: culicidae) reared from eggs collected with ovitraps ...201930776139
interventions for the control of aedes aegypti in latin america and the caribbean: systematic review and meta-analysis.to determine the effectiveness and degree of implementation of interventions for the control of aedes aegypti in latin america and the caribbean (lac) as reported in scientific literature.201930771267
juvenile hormone controls ovarian development in female anopheles albimanus mosquitoes.anophelinae mosquitoes are vectors of human malaria, a disease that infects hundreds of millions of people and causes almost 600,000 fatalities annually. despite their medical importance, laboratory studies on key aspects of anophelinae reproductive biology have been limited, and in particular, relatively little is known about the role of juvenile hormone (jh) in the control of female reproduction. the study presented here attempts to fill a gap of knowledge in our understanding of the jh contro ...201930765796
female resistance and harmonic convergence influence male mating success in aedes aegypti.despite the importance of mosquito mating biology to reproductive control strategies, a mechanistic understanding of individual mating interactions is currently lacking. using synchronised high-speed video and audio recordings, we quantified behavioural and acoustic features of mating attempts between tethered female and free-flying male aedes aegypti. in most couplings, males were actively displaced by female kicks in the early phases of the interaction, while flight cessation prior to adoption ...201930765779
surveillance of aedes aegypti indoors and outdoors using autocidal gravid ovitraps in south texas during local transmission of zika virus, 2016 to 2018.the yellow fever mosquito, aedes aegypti, has facilitated the re-emergence of dengue virus (denv) and emergence of chikungunya virus (chikv) and zika virus (zikv) in the americas and the caribbean. the recent transmission of these arboviruses in the continental united states has been limited, to date, to south florida and south texas despite ae. aegypti occurring over a much larger geographical region within the country. the main goal of our study was to provide the first long term longitudinal ...201930763563
potential for sylvatic and urban aedes mosquitoes from senegal to transmit the new emerging dengue serotypes 1, 3 and 4 in west africa.dengue fever (den) is the most common arboviral disease in the world and dengue virus (denv) causes 390 million annual infections around the world, of which 240 million are inapparent and 96 million are symptomatic. during the past decade a changing epidemiological pattern has been observed in africa, with den outbreaks reported in all regions. in senegal, all denv serotypes have been reported. these important changes in the epidemiological profile of den are occurring in a context where there i ...201930759080
exome-wide association of deltamethrin resistance in aedes aegypti from mexico.aedes aegypti is the major vector of a number of arboviruses that cause disease in humans. without vaccines or pharmaceuticals, pyrethroid insecticides remain the major tool for public health protection. pyrethroid resistance is now widespread. replacement substitutions in the voltage-gated sodium channel (vgsc) that reduce the stability of pyrethroid binding account for most of the resistance, but metabolic mechanisms also inactivate pyrethroids. high-throughput sequencing and the a. aegypti l5 ...201930758862
risk of dengue transmission in cocody (abidjan, ivory coast).in recent years, an upsurge of vector-borne diseases has been reported in several parts of the world. among these is dengue fever, the first arbovirus transmitted by mosquitoes of the genus aedes. after the detection of the dengue virus serological types (type 1, 2, and 3) in the health district of cocody-bingerville in ivory coast, entomological investigations were carried out in the city of cocody (host of most cases) to evaluate the risk of transmission of the disease in view of an effective ...201930755798
[effect of attraction of transfluthrin and linalool on the mosquito vector aedes aegypti]. 201930753763
citywide control of aedes aegypti (diptera: culicidae) during the 2016 zika epidemic by integrating community awareness, education, source reduction, larvicides, and mass mosquito trapping.this investigation was initiated to control aedes aegypti and zika virus transmission in caguas city, puerto rico, during the 2016 epidemic using integrated vector management (ivm), which included community awareness and education, source reduction, larviciding, and mass-trapping with autocidal gravid ovitraps (ago). the epidemic peaked in august to october 2016 and waned after april 2017. there was a preintervention period in october/november 2016 and ivm lasted until august 2017. the area unde ...201930753539
quantitative evaluation of the behavioral response to attractant and repellent compounds in anopheles pseudopunctipennis and aedes aegypti (diptera: culicidae) larvae.the mosquito anopheles pseudopunctipennis (theobald) is the principal vector for malaria in latin-america. aedes aegypti (l.) (diptera: culicidae) is the key vector of four important arboviral diseases: dengue, yellow fever, zika, and chikungunya. controlling larval stages to reduce the production of new adult mosquitoes is part of the integrated vector management strategies. however, there are few studies about the olfactory behavior on immature stages of mosquitoes, especially in an. pseudopun ...201930753516
vector competence of biting midges and mosquitoes for shuni virus.shuni virus (shuv) is an orthobunyavirus that belongs to the simbu serogroup. shuv was isolated from diverse species of domesticated animals and wildlife, and is associated with neurological disease, abortions, and congenital malformations. recently, shuv caused outbreaks among ruminants in israel, representing the first incursions outside the african continent. the isolation of shuv from a febrile child in nigeria and seroprevalence among veterinarians in south africa suggests that the virus ma ...201930753187
feasibility of feeding aedes aegypti mosquitoes on dengue virus-infected human volunteers for vector competence studies in iquitos, peru.transmission of dengue virus (denv) from humans to mosquitoes represents a critical component of dengue epidemiology. examinations of this process have generally been hampered by a lack of methods that adequately represent natural acquisition of denv by mosquitoes from humans. in this study, we assessed artificial and natural blood feeding methods based on rates of denv infection and dissemination within mosquitoes for use in a field-based epidemiological cohort study in iquitos, peru.201930753180
bacteria: a novel source for potent mosquito feeding-deterrents.antibiotic and insecticidal bioactivities of the extracellular secondary metabolites produced by entomopathogenic bacteria belonging to genus xenorhabdus have been identified; however, their novel applications such as mosquito feeding-deterrence have not been reported. here, we show that a mixture of compounds isolated from xenorhabdus budapestensis in vitro cultures exhibits potent feeding-deterrent activity against three deadly mosquito vectors: aedes aegypti, anopheles gambiae, and culex pipi ...201930746455
the long and short of hearing in the mosquito aedes aegypti.mating behavior in aedes aegypti mosquitoes occurs mid-air and involves the exchange of auditory signals at close range (millimeters to centimeters) [1-6]. it is widely assumed that this intimate signaling distance reflects short-range auditory sensitivity of their antennal hearing organs to faint flight tones [7, 8]. to the contrary, we show here that male mosquitoes can hear the female's flight tone at surprisingly long distances-from several meters to up to 10 m-and that unrestrained, resting ...201930744970
detection of rna-dependent rna polymerase of hubei reo-like virus 7 by next-generation sequencing in aedes aegypti and culex quinquefasciatus mosquitoes from brazil.advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. hubei reo-like virus 7 (hrlv 7) was recently detected by the virome analysis of fecal samples from migratory birds in australia. we now report the detection of rna-dependent rna polymerase sequences of hrlv 7 in pools of aedes aegypti and culex quinquefasciatus mosquitoes species from the brazilian amazon forest. phylogenetic ...201930744159
modeling the present and future distribution of arbovirus vectors aedes aegypti and aedes albopictus under climate change scenarios in mainland china.aedes aegypti and aedes albopictus are two important mosquito species which transmit various infectious arbovirus diseases represented mainly by dengue fever. these two species of mosquito have a wide range of distribution and strong transfer capacity. with ongoing global climate change, we are facing an increasing public health threat from the rapid spread of vectors in wider geographical areas. based on observed occurrence records of ae. aegypti and ae. albopictus and high-resolution environme ...201930743113
acceptability of aedes aegypti blood feeding on dengue virus-infected human volunteers for vector competence studies in iquitos, peru.as part of a study to investigate drivers of dengue virus (denv) transmission dynamics, this qualitative study explored whether denv-infected residents of iquitos, peru, considered it acceptable (1) to participate in direct mosquito feeding experiments (lab-reared aedes aegypti mosquitoes fed directly on human volunteers) and (2) to provide blood meals indirectly (ae. aegypti fed on blood drawn from participants by venipuncture). twelve focus group discussions (fgds; 94 participants: 82 females ...201930742621
mathematical modeling of dengue epidemic: control methods and vaccination strategies.dengue is, in terms of death and economic cost, one of the most important infectious diseases in the world. so, its mathematical modeling can be a valuable tool to help us to understand the dynamics of the disease and to infer about its spreading by the proposition of control methods. in this paper, control strategies, which aim to eliminate the aedes aegypti mosquito, as well as proposals for the vaccination campaign are evaluated. in our mathematical model, the mechanical control is accomplish ...201930740641
small-molecule agonists of ae. aegypti neuropeptide y receptor block mosquito biting.female aedes aegypti mosquitoes bite humans to obtain blood to develop their eggs. remarkably, their strong attraction to humans is suppressed for days after the blood meal by an unknown mechanism. we investigated a role for neuropeptide y (npy)-related signaling in long-term behavioral suppression and discovered that drugs targeting human npy receptors modulate mosquito host-seeking. in a screen of all 49 predicted ae. aegypti peptide receptors, we identified npy-like receptor 7 (npylr7) as the ...201930735632
the perfect appetizer: a pharmacological strategy for a non-biting mosquito.new possibilities for vector-borne disease control are revealed by duvall et al. (2019), who link host-seeking behavioral modulation in aedes aegypti to neuropeptide y (npy)-like receptor 7. small-molecule screening yields agonist compounds able to activate npylr7 and suppress attraction to hosts.201930735629
loop-mediated isothermal amplification (lamp) for the identification of invasive aedes mosquito species.invasive aedes mosquito species (diptera: culicidae) are of public health concern in europe because they are either recognized or potential vectors of pathogens. loop-mediated isothermal amplification (lamp) is a rapid and simple method for amplifying dna with high specificity and efficiency, with the technique having potential for application in the field, including in high-throughput format. specific lamp assays based on rdna internal transcribed spacers 1 or 2 sequences, considering intraspec ...201930734975
adaptation of aedes aegypti to salinity: characterized by larger anal papillae in larvae. 201930618451
epidemic potential of zika virus in australia: implications for blood transfusion safety.zika virus (zikv) is transfusion-transmissible. in australia the primary vector, aedes aegypti, is established in the north-east, such that local transmission is possible following importation of an index case, which has the potential to impact on blood transfusion safety and public health. we estimated the basic reproduction number (r 0 ) to model the epidemic potential of zikv in australian locations, compared this with the ecologically similar dengue viruses (denv), and examined possible impl ...201930618208
larvicidal and pupicidal activities of eco-friendly phenolic lipid products from anacardium occidentale nutshell against arbovirus vectors.aedes aegypti and culex quinquefasciatus are vectors of diseases that constitute public health problems. the discovery of products capable of inhibiting their development which are less harmful to the environment would have a huge impact on vector control. here, natural cashew nut shell liquid (cnsl), technical cnsl, anacardic acid, cardanol, and cardol were isolated from anacardium occidentale and evaluated for larvicidal and pupicidal activity against ae. aegypti and cx. quinquefasciatus under ...201930610586
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