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evidence of vertical transmission of zika virus in field-collected eggs of aedes aegypti in the brazilian amazon.arboviruses are viruses transmitted to humans and other animals by the bite of hematophagous arthropods. infections caused by chikungunya virus (chikv), dengue virus (denv), zika virus (zikv), and the deadlier yellow fever virus (yfv) are current public health problems in several countries, mainly those located in tropical and subtropical regions. one of the main prevention strategies continues to be vector control, with the elimination of breeding sites and surveillance of infested areas. the u ...201830011278
transmission potential of mayaro virus in florida aedes aegypti and aedes albopictus mosquitoes.mayaro virus (mayv) is an emerging mosquito-borne arbovirus present in central and south america that causes arthralgia and febrile illness. domestic mosquitoes aedes aegypti (diptera: culicidae) and aedes albopictus are potential vectors of mayv that may allow for transmission to humans in urban settings. the present paper assesses susceptibility to infection, disseminated infection and transmission potential in florida ae. aegypti and ae. albopictus for mayv. oral infection was significantly h ...201830006976
the occurrence, diversity and blood feeding patterns of potential vectors of dengue and yellow fever in kacheliba, west pokot county, kenya.yellow fever (yf) and dengue (den) viruses are important re-emerging mosquito-borne viruses sharing similar vectors and reservoirs. the last documented yf outbreak in kenya occurred in 1992-95. however, yf virus is re-emerging in bordering countries including uganda, ethiopia and south sudan with the potential for spread to the neighboring regions in kenya. dengue is endemic in kenya with outbreaks being detected in various towns in the north and the coast. this study reports on the aedes (stego ...201830006028
synergy in the adulticidal efficacy of essential oils for the improvement of permethrin toxicity against aedes aegypti l. (diptera: culicidae).in a previous screening program for mosquitocides from local edible plants in thailand, essential oils (eos) of cyperus rotundus, alpinia galanga and cinnamomum verum, were found to possess promising adulticidal activity against aedes aegypti. with the aim of reducing usage of conventional insecticides and improving the management of resistant mosquito populations, this study was designed to determine the potential synergism in the adulticidal efficacy of eos on permethrin toxicity against ae. a ...201830005688
epidemiological significance of dengue virus genetic variation in mosquito infection dynamics.the kinetics of arthropod-borne virus (arbovirus) transmission by their vectors have long been recognized as a powerful determinant of arbovirus epidemiology. the time interval between virus acquisition and transmission by the vector, termed extrinsic incubation period (eip), combines with vector mortality rate and vector competence to determine the proportion of infected vectors that eventually become infectious. however, the dynamic nature of this process, and the amount of natural variation i ...201830005085
the c-type lectin domain gene family in aedes aegypti and their role in arbovirus infection.several medically important flaviviruses that are transmitted by mosquitoes have been shown to bind to the c-type lectin fold that is present in either vertebrate or invertebrate proteins. while in some cases this interaction is part of a neutralizing anti-viral immune response, many reports have implicated this as critical for successful virus entry. despite the establishment of mosquito c-type lectin domain containing proteins (ctldcps) as known host factors in assisting the infectious process ...201830002303
optimization of cationic (q)-paper for detection of arboviruses in infected mosquitoes.previously (glushakova et al. 2017), a cellulose-based cationic (q) paper derivatized with quaternary ammonium groups was shown to be a convenient platform to collect, preserve, and store nucleic acids (nas) derived from mosquito vectors infected with pathogens for surveillance. nas bind electrostatically to q-paper, but the quantity of na bound depends on the paper's binding capacity. to optimize the original technology for mosquito surveillance, factors that affected na absorbance on q-paper w ...201830099053
discovery and high prevalence of phasi charoen-like virus in field-captured aedes aegypti in south china.arboviruses have caused significant global health concerns during the past decade. in this regard, continuous viral surveillance is essential to timely identify emerging arboviruses and other novel viruses. here, a novel isolate of phasi charoen-like virus (pclv zhanjiang01) was identified from field-captured aedes aegypti mosquitoes in zhanjiang by next generation sequencing. phylogenetic analysis suggested that pclv zhanjiang01 belonged to the genus phasivirus in the family phenuiviridae. the ...201830077072
managing aedes aegypti populations in the first zika transmission zones in the continental united states.the african zika virus swept across the pacific, reaching the new world in 2014. in july, 2016, miami-dade county, florida became the locus of the first mosquito-borne zika transmission zones in the continental united states. control efforts were guided by the centers for disease control and prevention, including aerial and truck sprays of adulticides and larvicides. to improve our understanding of how best to fight zika transmission in an urban environment in the developed world, trap counts of ...201830075097
genetic characterization of chikungunya viruses isolated during the 2015-2017 outbreaks in different states of india, based on their e1 and e2 genes.during 2015-2017, chikungunya virus (chikv) showed a resurgence in several parts of india with karnataka, maharashtra and new delhi accounting for a majority of the cases. e2-e1 gene based characterization revealed indian subcontinent sublineage strains possessing aedes aegypti mosquito-adaptive mutations e1: k211e and e2:v264a, with the 211 site positively selected. novel mutational sites e1: k16e/q, e1: k132q/t, e1: s355t, e2: c19r and e2:s185y could be associated with epitopes or virulence de ...201830073419
a low-cost, battery-powered acoustic trap for surveilling male aedes aegypti during rear-and-release operations.the aedes aegypti mosquito is a primary vector of several serious arboviruses throughout the world and is therefore of great concern to many public health organizations. with vector control methodology pivoting towards rearing and releasing large numbers of genetically modified, sterilized, or wolbachia-infected male mosquitoes to control vector populations, economical surveillance methods for release tracking becomes increasingly necessary. previous work has identified that male ae. aegypti are ...201830071091
rapid evolution and the genomic consequences of selection against interspecific mating.while few species introduced into a new environment become invasive, those that do provide critical information on ecological mechanisms that determine invasions success and the evolutionary responses that follow invasion. aedes albopictus (the asian tiger mosquito) was introduced into the naturalized range of aedes aegypti (the yellow fever mosquito) in the united states in the mid-1980s, resulting in the displacement of a. aegypti in much of the south-eastern united states. the rapid displacem ...201830069966
small females prefer small males: size assortative mating in aedes aegypti mosquitoes.with aedes aegypti mosquitoes now being released in field programmes aimed at disease suppression, there is interest in identifying factors influencing the mating and invasion success of released mosquitoes. one factor that can increase release success is size: released males may benefit competitively from being larger than their field counterparts. however, there could be a risk in releasing only large males if small field females avoid these males and instead prefer small males. here we invest ...201830068363
a gustatory receptor used for rapid detection of tyrophagus putrescentiae in fungi hosts.the storage mite, tyrophagus putrescentiae, found worldwide in many habitats, is an important pest of edible fungi in china. storage mites are tiny and difficult to observe, especially when they occur in fungi composts. in this study, one gustatory receptor protein (tputgr1) was identified from the transcriptome of t. putrescentiae. phylogenetic analysis of grs families from 10 arthropod species revealed that tputgr1 had high homology with the sccagr1 of sarcoptes scabiei and turtgr1-2 of tetran ...201830061635
juvenile hormone-regulated alternative splicing of the taiman gene primes the ecdysteroid response in adult mosquitoes.juvenile hormone (jh) regulates many aspects of insect development and reproduction. in some processes, jh plays a critical role in defining the action of the steroid hormone 20-hydroxyecdysone (20e). in aedes aegypti mosquitoes, jh prepares newly emerged female adults to become competent to synthesize vitellogenin in response to 20e after blood ingestion. the molecular basis of this competence is still not well understood. here, we report that jh regulates pre-mrna splicing of the taiman gene, ...201830061397
comparing the effectiveness of different strains of wolbachia for controlling chikungunya, dengue fever, and zika.once aedes aegypti and aedes albopictus mosquitoes that spread chikungunya virus, dengue virus, and zika virus are infected with wolbachia, they have reduced egg laying rates, reduced transmission abilities, and shorter lifespans. since most infected mosquitoes are only infectious in the last few days of their lives, shortening a mosquito's lifespan by a day or two can greatly reduce their abilities to spread mosquito-borne viral diseases, such as chikungunya, dengue fever, and zika. we develope ...201830059498
modeling the importation and local transmission of vector-borne diseases in florida: the case of zika outbreak in 2016.chikungunya, dengue, and zika viruses are all transmitted by aedes aegypti and aedes albopictus mosquito species, had been imported to florida and caused local outbreaks. we propose a deterministic model to study the importation and local transmission of these mosquito-borne diseases. the purpose is to model and mimic the importation of these viruses to florida via travelers, local infections in domestic mosquitoes by imported travelers, and finally non-travel related transmissions to local huma ...201830053386
modifying the biogents sentinel trap to increase the longevity of captured aedes aegypti.aedes aegypti (l.) (diptera: culicidae) is a vector of viruses causing dengue, zika, chikungunya, and yellow fever and subsequently pose a significant global threat to public health. while sampling live mosquitoes is useful for surveillance purposes, most traps targeting aedes kill captured mosquitoes. the biogents sentinel (bgs) trap, the gold standard for capturing ae. aegypti, is one such trap. in our study, we modified the bgs trap to increase the survival of captured ae. aegypti by replacin ...201830053026
experimental study of the susceptibility of a european aedes albopictus strain to dengue virus under a simulated mediterranean temperature regime.dengue virus (denv) has re-emerged in europe driven by the geographic expansion of the mosquito species aedes albopictus and aedes aegypti (diptera: culicidae) and the introduction of the virus by viraemic travellers. in the present study, the vector competence (vc) of ae. albopictus collected in catalonia (northeast spain) was evaluated for two different denv strains, denv-1 and denv-2, the serotypes responsible for all outbreaks of dengue that have occurred in europe. mosquitoes were reared un ...201830051490
larvicidal effectiveness of azolla pinnata against aedes aegypti (diptera: culicidae) with its effects on larval morphology and visualization of behavioural response.dengue is vector-borne diseases with 390 million infections per year extending over 120 countries of the world. aedes aegypti (l.) (diptera: culicidae) is a primary vector for dengue viral infections for humans. current focus on application of natural product against mosquito vectors has been the main priority for research due to its eco-safety. the extensive use of chemical insecticides has led to severe health problems, environmental pollution, toxic hazards to human and nontarget species, and ...201830050688
the impact of the age of first blood meal and zika virus infection on aedes aegypti egg production and longevity.the impact of senescence and pathogen infection on aedes aegypti life-history traits remains poorly understood. this laboratory study focused on the impact of zika virus (zikv) infection and the age of first blood intake on blood meal and clutch sizes, and more importantly on the egg production ratio per μl of blood. three groups of zikv-infected and uninfected ae. aegypti females that received their first blood meal at 7 (young feeders), 14 (mature feeders) and 21 days old (old feeders) were mo ...201830048481
characterization of dengue virus in aedes aegypti and aedes albopictus spp. of mosquitoes: a study in khyber pakhtunkhwa, pakistan.dengue is a vector-borne disease caused by dengue virus. according to the recent report of cdc that one-third population of the world are at high risk with dengue fever. the prevalence of the dengue hemorrhagic fever was found more in tropical and sub-tropical regions of the world. aedes mosquitoes was reported as the main cause of transmission of dengue virus. so the current study was planned to characterize the virus in aedes mosquitoes collected from different area of pakistan. in current inv ...201830046621
specific human antibody responses to aedes aegypti and aedes polynesiensis saliva: a new epidemiological tool to assess human exposure to disease vectors in the pacific.aedes mosquitoes severely affect the health and wellbeing of human populations by transmitting infectious diseases. in french polynesia, aedes aegypti is the main vector of dengue, chikungunya and zika, and aedes polynesiensis the primary vector of bancroftian filariasis and a secondary vector of arboviruses. tools for assessing the risk of disease transmission or for measuring the efficacy of vector control programmes are scarce. a promising approach to quantify the human-vector contact relies ...201830040826
is autumn the key for dengue epidemics in non endemic regions? the case of argentina.dengue is a major and rapidly increasing public health problem. in argentina, the southern extreme of its distribution in the americas, epidemic transmission takes place during the warm season. since its re-emergence in 1998 two major outbreaks have occurred, the biggest during 2016. to identify the environmental factors that trigger epidemic events, we analyzed the occurrence and magnitude of dengue outbreaks in time and space at different scales in association with climatic, geographic and dem ...201830038860
the effect of skitosnack, an artificial blood meal replacement, on aedes aegypti life history traits and gut microbiota.public health research and vector control frequently require the rearing of large numbers of vector mosquitoes. all target vector mosquito species are anautogenous, meaning that females require vertebrate blood for egg production. vertebrate blood, however, is costly, with a short shelf life. to overcome these constraints, we have developed skitosnack, an artificial blood meal replacement for the mosquito aedes aegypti, the vector of dengue, zika and chikungunya virus. skitosnack contains bovine ...201830038361
classification of containers with aedes aegypti pupae using a neural networks model.this paper discusses the presence of aedes aegypti pupae in different types of containers considering: volume, ph of the container, among other variables.201830036370
acute inhalation toxicity study of n-heneicosane and its combination with diflubenzuron: an attracticide of aedes aegypti.combination of an oviposition pheromone and an insect growth regulator for the control of vectors is an effective approach. there is a need for toxicological evaluation before its introduction. the present study evaluates the acute inhalation toxicity of n-heneicosane and its combination with diflubenzuron in a head-only inhalation exposure chamber made of glass.201830033812
cgti, a novel thermostable kunitz trypsin-inhibitor purified from cassia grandis seeds: purification, characterization and termiticidal activity.cassia grandis trypsin inhibitor (cgti) is a novel plant serine proteinase inhibitor. this study sets out to purify a thermostable inhibitor from the seeds of cassia grandis and to provide biochemical information about a novel peptide belonging to the kunitz family. moreover, toxicity assays against artemia, aedes aegypti larvae-l4 and nasutitermes corniger are evaluated. the purification process was performed using acetone precipitation, trypsin-sepharose-cl4b and superdex-g75. the inhibitor sh ...201830030073
evaluation of standard field and laboratory methods to compare protection times of the topical repellents pmd and deet.mosquitoes are important vectors of pathogens, and travellers to disease endemic countries are advised to avoid bites by applying topical repellents. topical repellents are typically tested either in the arm-in-cage (aic) test under laboratory conditions or in the field, but not often under both conditions. we, therefore, investigated how two topical repellents, 15% para-menthane-3,8-diol (pmd) and 15% n,n-diethyl-3-methylbenzamide (deet) compare against each other both in the aic test against t ...201830135603
experimental evaluation of the impact of household aerosolized insecticides on pyrethroid resistant aedes aegypti.the extensive reliance on insecticides to control aedes aegypti mosquitoes and disrupt transmission of dengue, chikungunya and zika has fueled the emergence of widespread resistance to insecticides. mismatch between the frequency of pyrethroid resistance in mosquitoes and the occurrence of pyrethroid-based insecticide applications for vector control is often hypothesized to be due to household use of commercial insecticide products. we experimentally quantified phenotypic and genotypic responses ...201830135460
differential transmission of antiviral drug-resistant chikungunya viruses by aedes mosquitoes.the chikungunya virus (chikv) is transmitted by female aedes aegypti and aedes albopictus mosquitoes, mostly present in (sub)tropical regions. no antivirals are available to treat chikv infections. if antiviral drugs are proven efficient to treat chikv-infected patients, it will be pivotal to determine whether drug-resistant viruses can be transmitted from one human to another by their mosquito vectors. we orally infected aedes aegypti mosquitoes with a blood meal containing wild-type or drug-re ...201830135220
study of aedes aegypti population with emphasis on the gonotrophic cycle length and identification of arboviruses: implications for vector management in cemeteries.aedes aegypti is the vector of the arboviruses causing dengue, chikungunya and zika infections in mexico. however, its presence in public places has not been fully evaluated. in a cemetery from merida, yucatan, mexico, the productivity of ae. aegypti, the gonotrophic cycle, and the presence of ae. aegypti females infected with arboviruses were evaluated. immature and adult mosquitoes were inspected every two months between april 2016 to june 2017. for the gonotrophic cycle length, the daily patt ...201830133604
estimating the effects of variation in viremia on mosquito susceptibility, infectiousness, and r0 of zika in aedes aegypti.zika virus (zikv) is an arbovirus primarily transmitted by aedes mosquitoes. like most viral infections, zikv viremia varies over several orders of magnitude, with unknown consequences for transmission. to determine the effect of viral concentration on zikv transmission risk, we exposed field-derived ae. aegypti mosquitoes to four doses (10(3), 10(4), 10(5), 10(6) pfu/ml) representative of potential variation in the field. we demonstrate that increasing zikv dose in the blood-meal significantly ...201830133450
estimating air temperature using modis surface temperature images for assessing aedes aegypti thermal niche in bangkok, thailand.dengue, the most widespread urban vector-borne disease, is transmitted to human by the mosquito aedes aegypti. its distribution in urban areas is heterogeneous over time and space. in time, it is linked to seasonal variations such as warm and cold seasons, as well as rainy and dry seasons. in space, it is linked to social and environmental conditions, which alternate between rich and deprived neighborhoods, vegetated and densely built areas. these variations in terms of land cover can affect sur ...201830132225
efficacy of culicinomyces spp. against aedes aegypti eggs, larvae and adults.the aquatic fungal genus culicinomyces attacks dipteran larvae but little is known about its efficacy against aedes aegypti. here we report on the activity of both described species-culicinomyces clavisporus and culicinomyces bisporalis-on larvae, eggs and adults, and on trans-stadial transmission. ten c. clavisporus isolates (arsef 372, 582, 644, 706, 964, 1260, 2471, 2478, 2479 and 2480) and c. bisporalis arsef 1948 were screened against larvae of this important vector of viral diseases. arsef ...201830130538
glyphosate impairs learning in aedes aegypti mosquito larvae at field-realistic doses.glyphosate is the most widely used herbicide in the world. over the past few years, the number of studies revealing deleterious effects of glyphosate on non-target species has been increasing. here, we studied the impact of glyphosate at field-realistic doses on learning in mosquito larvae (aedes aegypti). larvae of a. aegypti live in small bodies of water and perform a stereotyped escape response when a moving object projects its shadow on the water surface. repeated presentations of an innocuo ...201830127074
laboratory selection of aedes aegypti field populations with the organophosphate malathion: negative impacts on resistance to deltamethrin and to the organophosphate temephos.resistance to pyrethroids and to the organophosphate temephos is widespread in brazilian populations of the dengue vector, aedes aegypti. thereof, since 2009 insect growth regulators are employed as larvicides, and malathion is used against adults.201830125295
travel-associated zika cases and threat of local transmission during global outbreak, california, usa.zika and associated microcephaly among newborns were reported in brazil during 2015. zika has since spread across the americas, and travel-associated cases were reported throughout the united states. we reviewed travel-associated zika cases in california to assess the potential threat of local zika virus transmission, given the regional spread of aedes aegypti and ae. albopictus mosquitoes. during november 2015-september 2017, a total of 588 travel-associated zika cases were reported in californ ...201830124194
chicory (cichorium intybus) and wormwood (artemisia absinthium) extracts exhibit strong larvicidal activity against mosquito vectors of malaria, dengue fever, and filariasis.vector-borne diseases transmitted by mosquitoes cause globally important diseases such as malaria, dengue fever, and filariasis. the incidence of these diseases can be reduced through mosquito control programs but these control programs currently rely on synthetic insecticides that can impact the environment, and has selected widespread mosquito resistance. environment friendly and biodegradable natural insecticides discovered in plants offer an alternative approach to mosquito control. here, we ...201830118862
colonized sabethes cyaneus, a sylvatic new world mosquito species, shows a low vector competence for zika virus relative to aedes aegypti.the introduction of zika virus (zikv) to the americas raised concern that the virus would spill back from human transmission, perpetuated by aedes aegypti, into a sylvatic cycle maintained in wildlife and forest-living mosquitoes. in the americas, sabethes species are vectors of sylvatic yellow fever virus (yfv) and are therefore candidate vectors of a sylvatic zikv cycle. to test the potential of sabethes cyaneus to transmit zikv, sa. cyaneus and ae. aegypti were fed on a129 mice one or two day ...201830115888
levels of resistance to pyrethroid among distinct kdr alleles in aedes aegypti laboratory lines and frequency of kdr alleles in 27 natural populations from rio de janeiro, brazil.several mutations in voltage gated sodium channel (nav) have been identified in aedes aegypti populations worldwide. however, only few are related to knockdown resistance to pyrethroids, most of which with variations in the 1016 and 1534 nav sites. in brazil, at least two nav alleles are known: navr1, with a substitution in the 1534 (1016 val+ + 1534 ile kdr ) and navr2, with substitutions in both 1016 and sites (1016ilekdr + 1534cys kdr ). there is also the duplication in the nav gene, with one ...201830112367
oviplate: a convenient and space-saving method to perform individual oviposition assays in aedes aegypti.aedes aegypti is the principal vector of the urban arboviruses and the blood ingestion is important to produce the eggs in this species. to analyze the egg production in ae. aegypti, researchers frequently use small cages or drosophila vials to collect eggs from gravid females. although it is affordable, the setup is time- and space-consuming, mainly when many mosquitoes need to be individually analyzed. this study presents an easy, cheap, and space-saving method to perform individual ovipositio ...201830111702
temperature drives zika virus transmission: evidence from empirical and mathematical models.temperature is a strong driver of vector-borne disease transmission. yet, for emerging arboviruses we lack fundamental knowledge on the relationship between transmission and temperature. current models rely on the untested assumption that zika virus responds similarly to dengue virus, potentially limiting our ability to accurately predict the spread of zika. we conducted experiments to estimate the thermal performance of zika virus (zikv) in field-derived aedes aegypti across eight constant temp ...201830111605
zika might not be acting alone: using an ecological study approach to investigate potential co-acting risk factors for an unusual pattern of microcephaly in brazil.zika virus infections can cause a range of neurologic disorders including congenital microcephaly. however, while zika infections have been notified across all regions in brazil, there has been an unusual number of congenital microcephaly case notifications concentrated in the northeast of the country. to address this observation, we investigated epidemiological data (2014-2016) on arbovirus co-distribution, environmental and socio-economic factors for each region in brazil. data on arbovirus re ...201830110370
parental and offspring larval diets interact to influence life-history traits and infection with dengue virus in aedes aegypti.the environmental conditions experienced by parents can influence offspring phenotype along with the conditions experienced by offspring. these parental effects are clear in organisms that display parental care and are less clear in other organisms. here, we consider effects of parental and offspring larval nutrition on offspring development time, survivorship and infection with dengue virus in aedes aegypti, the mosquito vector of dengue, chikungunya, yellow fever and zika. parents were raised ...201830109101
anti-inflammatory activities of aedes aegypti cecropins and their protection against murine endotoxin shock.mosquitoes are armed with physiologically active compounds to suppress the host immunity including host inflammatory reaction. however, the specific anti-inflammatory components in mosquitoes remain unknown.201830107813
yellow fever in africa and the americas: a historical and epidemiological perspective.yellow fever was transported during the slave trade in the 15th and 16th centuries from africa to the americas where the virus encountered favorable ecological conditions that allowed creation of a sustainable sylvatic cycle. despite effective vector control and immunization programs for nearly a century, yellow fever epidemics reemerged in many latin american countries, particularly brazil. the emergence or reemergence of vector-borne diseases encompasses many intricate factors. yellow fever ou ...201830158957
local and regional dynamics of chikungunya virus transmission in colombia: the role of mismatched spatial heterogeneity.mathematical models of transmission dynamics are routinely fitted to epidemiological time series, which must inevitably be aggregated at some spatial scale. weekly case reports of chikungunya have been made available nationally for numerous countries in the western hemisphere since late 2013, and numerous models have made use of this data set for forecasting and inferential purposes. motivated by an abundance of literature suggesting that the transmission of this mosquito-borne pathogen is local ...201830157921
sublethal effects of a vapour-active pyrethroid, transfluthrin, on aedes aegypti and ae. albopictus (diptera: culicidae) fecundity and oviposition behaviour.it is assumed that mosquitoes surviving exposure to spatial repellents when attempting to bite a host will not have significant adverse impacts on their downstream biology. therefore, a critical knowledge gap is understanding the extent to which sublethal exposure to volatile pyrethroids may damage the performance of mosquitoes that survive exposure to vapour-active pyrethroids. to address this, laboratory-reared aedes aegypti (l.) and ae. albopictus (skuse) were exposed to one of three subletha ...201830157907
zika infection decreases aedes aegypti locomotor activity but does not influence egg production or viability.zika has emerged as a new public health threat after the explosive epidemic in brazil in 2015. it is an arbovirus transmitted mainly by aedes aegypti mosquitoes. the knowledge of physiological, behavioural and biological features in virus-infected vectors may help the understanding of arbovirus transmission dynamics and elucidate their influence in vector capacity.201830156598
oviposition deterrent efficacy and characteristics of a botanical natural product, ocimum gratissimum (l.) oil-alginate beads, against aedes aegypti (l.).this study was aimed at investigating the oviposition deterrent activity of ocimum gratissimum (l.) essential oil (o. gratissimum oil) and its product, ocimum gratissimum (l.)- alginate beads (beads), against aedes aegypti (ae. aegypti) mosquitoes. chemical analysis of o. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and z-β-ocimene (14.95 %) as major constituents. good characteristics of beads were obtained by th ...201830154681
forced salivation as a method to analyze vector competence of mosquitoes.vector competence is defined as the potential of a mosquito species to transmit a mosquito-borne virus (mobovirus) to a vertebrate host. viable virus particles are transmitted during a blood meal via the saliva of an infected mosquito. forced salivation assays allow determining the vector potential on the basis of single mosquitoes, avoiding the use of animal experiments. the method is suitable to analyze a large number of mosquitoes in one experiment within a short period of time. forced saliva ...201830148488
qsar modelling of synergists to increase the efficacy of deltamethrin against pyrethroid-resistant aedes aegypti mosquitoes$.space spraying of deltamethrin allows the control of adult aedes (stegomyia) aegypti mosquitoes. unfortunately, many vector control programs are threatened by the development of resistances that decrease the efficacy of this adulticide. faced with this situation, we can either try to use another insecticide presenting a different mechanism of action or find a strategy that brings back the efficacy of the insecticide at a satisfying level to pursue its use in vector control. restoration of the ef ...201830141356
transcriptomic analysis of aedes aegypti in response to mosquitocidal bacillus thuringiensis llp29 toxin.globally, aedes aegypti is one of the most dangerous mosquitoes that plays a crucial role as a vector for human diseases, such as yellow fever, dengue, and chikungunya. to identify (1) transcriptomic basis of midgut (2) key genes that are involved in the toxicity process by a comparative transcriptomic analysis between the control and bacillus thuringiensis (bt) toxin (llp29 proteins)-treated groups. next-generation sequencing technology was used to sequence the midgut transcriptome of a. aegypt ...201830140020
impedimetric immunosensor for dengue diagnosis using graphite screen-printed electrodes coated with poly(4-aminophenylacetic acid).dengue is an infectious viral disease transmitted by the aedes aegypti mosquito, the control of which is complex. in addition, the clinical diagnosis is difficult to perform since it resembles other febrile infections; thus, the development of more effective methods to detect dengue virus (dv) has drawn increasing attention. the present study aimed to develop an impedimetric immunosensor for dengue diagnosis using a screen-printed electrode (spe) functionalized with polymer films derived from 4- ...201830182296
dengue virus infection changes aedes aegypti oviposition olfactory preferences.aedes aegypti mosquitoes, main vectors for numerous flaviviruses, have olfactory preferences and are capable of olfactory learning especially when seeking their required environmental conditions to lay their eggs. in this study, we showed that semiochemical conditions during aedes aegypti larval rearing affected future female choice for oviposition: water-reared mosquitoes preferred to lay eggs in water or p-cresol containers, while skatole reared mosquitoes preferred skatole sites. using two in ...201830181545
a conceptual model for optimizing vaccine coverage to reduce vector-borne infections in the presence of antibody-dependent enhancement.many vector-borne diseases co-circulate, as the viruses from the same family are also transmitted by the same vector species. for example, zika and dengue viruses belong to the same flavivirus family and are primarily transmitted by a common mosquito species aedes aegypti. zika outbreaks have also commonly occurred in dengue-endemic areas, and co-circulation and co-infection of both viruses have been reported. as recent immunological cross-reactivity studies have confirmed that convalescent plas ...201830173664
demonstration of efficient vertical and venereal transmission of dengue virus type-2 in a genetically diverse laboratory strain of aedes aegypti.aedes aegypti is the primary mosquito vector of dengue viruses (denv; serotypes 1-4). human-mosquito transmission cycles maintain denv during epidemics but questions remain regarding how these viruses survive when human infections and vector abundance are minimal. aedes mosquitoes can transmit denv within the vector population through two alternate routes: vertical and venereal transmission (vt and vnt, respectively). we tested the efficiency of vt and vnt in a genetically diverse laboratory (gd ...201830169505
the surveillance of chikungunya virus in a temperate climate: challenges and possible solutions from the experience of lazio region, italy.chikv has become an emerging public health concern in the temperate regions of the northern hemisphere as a consequenceof the expansion of the endemic areas of its vectors (mainly aedes aegypti and aedesalbopictus). in 2017, a new outbreak of chikv was detected in italy with three clusters of autochthonous transmission in the lazio region (central italy), in the cities of anzio, rome, and latina and a secondary cluster in the calabria region (south italy). given the climate characteristics of it ...201830223536
where vectors collide: the importance of mechanisms shaping the realized niche for modeling ranges of invasive aedes mosquitoes.the vector mosquitoes aedes aegypti (l.), native to africa, and aedes albopictus (skuse), native to asia, are widespread invasives whose spatial distributions frequently overlap. predictive models of their distributions are typically correlative rather than mechanistic, and based on only abiotic variables describing putative environmental requirements despite extensive evidence of competitive interactions leading to displacements. here we review putative roles of competition contributing to dist ...201830220875
projecting the end of the zika virus epidemic in latin america: a modelling analysis.zika virus (zikv) emerged in latin america and the caribbean (lac) region in 2013, with serious implications for population health in the region. in 2016, the world health organization declared the zikv outbreak a public health emergency of international concern following a cluster of associated neurological disorders and neonatal malformations. in 2017, zika cases declined, but future incidence in lac remains uncertain due to gaps in our understanding, considerable variation in surveillance and ...201830285863
relationship between local presence and density of aedes aegypti eggs with dengue cases: a spatial analysis approach.to analyze the relationship between the occurrence of dengue and ovitrap positivity and dengue egg density in two amazonas municipalities (brazil) in 2016.201830282110
potential of aedes aegypti populations in madeira island to transmit dengue and chikungunya viruses.aedes spp. mosquitoes mainly transmit the arboviruses dengue virus (denv) and chikungunya virus (chikv) in urban areas, causing a severe public health problem. in 2012-2013, a major dengue outbreak occurred on madeira island where the mosquito aedes aegypti was the only vector. up to now, the competence of ae. aegypti populations from madeira to transmit denv or chikv remains unknown. this study aimed to assess experimentally the ability of ae. aegypti populations from madeira to transmit these ...201830208974
distribution and breeding sites of aedes aegypti and aedes albopictus in 32 urban/peri-urban districts of mozambique: implication for assessing the risk of arbovirus outbreaks.aedes-borne arboviruses have emerged as an important public health problem worldwide and, in mozambique, the number of cases and its geographical spread have been growing. however, information on the occurrence, distribution and ecology of aedes aegypti and ae. albopictus mosquitoes remain poorly known in the country.201830208017
mosquito and tick repellency of two anthemis essential oils from saudi arabia.the essential oils (eos) of anthemis melampodina (am) and anthemis scrobicularis (as) (asteraceae) were extracted from the aerial parts of the plants by hydrodistillation, and their chemical compositions were analyzed using gc-fid and gc-ms. fifty-six components representing 85.5% of the oil composition of anthemis melampdina were identified, and the major components were α-pinene (17.1%) and β-eudesmol (13.8%). forty-one components representing 86% of the oil composition of anthemis scrobicular ...201830202228
the effect of permethrin resistance on aedes aegypti transcriptome following ingestion of zika virus infected blood.aedes aegypti (l.) is the primary vector of many emerging arboviruses. insecticide resistance among mosquito populations is a consequence of the application of insecticides for mosquito control. we used rna-sequencing to compare transcriptomes between permethrin resistant and susceptible strains of florida ae. aegypti in response to zika virus infection. a total of 2459 transcripts were expressed at significantly different levels between resistant and susceptible ae. aegypti. gene ontology analy ...201830200481
quiescence in aedes aegypti: interpopulation differences contribute to population dynamics and vectorial capacity.the strategy of aedes aegypti to prolong embryonic viability by quiescence has severe implications for geographic expansion and maintenance of mosquito populations in areas under control measures. we evaluated the effects of quiescence on biological parameters directly or indirectly associated with population dynamics and vectorial capacity in populations of this mosquito species from two brazilian municipalities characterized as dengue, chikungunya, and zika transmission areas. egg viability, i ...201830200417
nutritional quality during development alters insulin-like peptides' expression and physiology of the adult yellow fever mosquito, aedes aegypti.mosquitoes have distinct developmental and adult life history, and the vectorial capacity of females has been shown to be affected by the larval nutritional environment. however, little is known about the effect of developmental nutrition on insulin-signaling and nutrient storage. in this study, we used aedes aegypti, the yellow fever mosquito, to determine whether larval nutrition affects insulin gene expression. we also determined the traits regulated by insulin signaling, such as stored-nutri ...201830200185
evaluation of eosin-methylene blue as a photosensitizer for larval control of aedes aegypti by a photodynamic process.aedes aegypti (ae. aegypti) is a competent vector for transmitting important viral diseases such as yellow fever, dengue, chikungunya, and zika. several strategies have been applied to avoid ae. aegypti proliferation by using environmental management, biological, and chemical approaches. however, the development of new methods for effective control of the insect vector population is still needed. photodynamic control is an alternative way to control the vector population by using a physical appr ...201830200177
multiple insecticide resistance mechanisms in primary dengue vector, aedes aegypti (linn.) from dengue endemic districts of sub-himalayan west bengal, india.mosquitoes belonging to genus aedes are the prime vectors of several arboviral diseases such as dengue, zika and chikungunya worldwide. every year numerous cases of dengue infections occur throughout the world, proper control of which depends on efficient vector control. however the onset of insecticide resistance has resulted in failure of vector control approaches.201830199543
plant-derived compounds regulate formation of the insect juvenile hormone receptor complex.insect growth regulators (igrs) are attractive pest control agents due to their high target specificity and relative safety to the environment. recently, plants have been shown to synthesize igrs that affect the insect juvenile hormone (jh) as a part of their defense mechanisms. using a yeast two-hybrid system transformed with the aedes aegypti jh receptor as a reporter system, we identified several jh agonists (jhas) and antagonists (jhans) causing retardation in the ovarian development of fema ...201830195384
extensive recoding of dengue virus type 2 specifically reduces replication in primate cells without gain-of-function in aedes aegypti mosquitoes.dengue virus (denv), an arthropod-borne ("arbovirus") virus, causes a range of human maladies ranging from self-limiting dengue fever to the life-threatening dengue shock syndrome and proliferates well in two different taxa of the animal kingdom, mosquitoes and primates. mosquitoes and primates show taxonomic group-specific intolerance to certain codon pairs when expressing their genes by translation. this is called "codon pair bias". by necessity, dengue viruses evolved to delicately balance th ...201830192757
differential proteome analysis of chikungunya virus and dengue virus coinfection in aedes mosquitoes.chikungunya virus (chikv) and dengue virus (denv) are important arboviruses transmitted by aedes mosquitoes. these viruses are known to coexist within the same vector and coinfect the same host. although information is available on the mechanism of replication of chikv and denv when present independently in a vector, reports are lacking on the dynamics of virus-vector interactions when these viruses coexist in a mosquito. the current study attempts to understand the perturbations in the proteome ...201830192139
recent advances in threshold-dependent gene drives for mosquitoes.mosquito-borne diseases, such as malaria, dengue and chikungunya, cause morbidity and mortality around the world. recent advances in gene drives have produced control methods that could theoretically modify all populations of a disease vector, from a single release, making whole species less able to transmit pathogens. this ability has caused both excitement, at the prospect of global eradication of mosquito-borne diseases, and concern around safeguards. drive mechanisms that require individuals ...201830190331
evaluation of (-)-borneol derivatives against the zika vector, aedes aegypti and a non-target species, artemia sp.zika, dengue, and chikungunya are vector-borne diseases of pronounced concern transmitted by the mosquito aedes aegypti linn. (diptera: culicidae). the most important method to avoid outbreaks is to control mosquito spreading by the employment of insecticides and larvicides. failure to control mosquito dispersal is mostly accounted to ae. aegypti resistance to currently available larvicides and insecticides, encouraging the development of novel pesticides. in addition, the excessive use of larvi ...201830187415
knowledge, practices and entomological aspects of dengue in medellín, colombia: a comparative study of neighborhoods with high and low incidenceintroduction: dengue, mainly transmitted by aedes aegypti, is a very important viral disease in terms of public health. colombia is an endemoepidemic country for dengue and, in cities like medellín, there are neighborhoods with high and low incidence. the disease dynamics in the neighborhoods might be determined by differences in the knowledge, practices and entomological aspects of the vector among the communities.objective: to identify the knowledge and practices of residents of neighborhoods ...201830184371
novel larvicide tablets of bacillus thuringiensis var. israelensis: assessment of larvicidal effect on aedes aegypti (diptera: culicidae) in colombia.aedes (stegomyia) aegypti is the vector for dengue, chikungunya, and zika arboviruses. bti-cecif is a bioinsecticide designed and developed in the form of a solid tablet for the control of this vector. it contains bacillus thuringiensis var. israelensis (bti) serotype h-14.201830184370
exposure and sensitization to insects in allergic patients in the tropicsintroduction: mites are an important source of allergens in the tropics. other potential sources of allergens prevalent in the region such as insects have been poorly studied.objective: to determine the relationship between exposure and allergic sensitization to cockroaches, mosquitos, ants and the interaction with mite sensitization.materials and methods: we included patients with allergy tests for blatella germanica, aedes aegypti, solenopsis invicta, blomia tropicalis, dermatophagoides farina ...201830184366
genetic variability of aedes aegypti in the department of sucre, colombia, by analysis of the nucleotide sequence of the mitochondrial nd4 geneintroduction. aedes aegypti is the most important mosquito species in america for the transmission of viruses of dengue, zika, chikungunya and yellow fever. ecological factors as well as chemical controls can affect the genetic composition of ae. aegypti populations, which is why its genetic characterization is necessary.objective. to determine the genetic variability of ae. aegypti populations in four municipalities of sucre department, colombia.materials and methods. larvae of ae. aegypti, col ...201830184356
vector competence of aedes bromeliae and aedes vitattus mosquito populations from kenya for chikungunya virus.kenya has experienced outbreaks of chikungunya in the past years with the most recent outbreak occurring in mandera in the northern region in may 2016 and in mombasa in the coastal region from november 2017 to february 2018. despite the outbreaks in kenya, studies on vector competence have only been conducted on aedes aegypti. however, the role played by other mosquito species in transmission and maintenance of the virus in endemic areas remains unclear. this study sought to determine the possib ...201830321181
using maldi-tof ms to identify mosquitoes collected in mali and their blood meals.matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) has been recently described as an innovative and effective tool for identifying arthropods and mosquito blood meal sources. to test this approach in the context of an entomological survey in the field, mosquitoes were collected from five ecologically distinct areas of mali. we successfully analysed the blood meals from 651 mosquito abdomens crushed on whatman filter paper (wfps) in the field using maldi-t ...201829409547
modulation of host learning in aedes aegypti mosquitoes.how mosquitoes determine which individuals to bite has important epidemiological consequences. this choice is not random; most mosquitoes specialize in one or a few vertebrate host species, and some individuals in a host population are preferred over others. mosquitoes will also blood feed from other hosts when their preferred is no longer abundant, but the mechanisms mediating these shifts between hosts, and preferences for certain individuals within a host species, remain unclear. here, we sho ...201829395917
the spatial and temporal scales of local dengue virus transmission in natural settings: a retrospective analysis.dengue is a vector-borne disease caused by the dengue virus (denv). despite the crucial role of aedes mosquitoes in denv transmission, pure vector indices poorly correlate with human infections. therefore there is great need for a better understanding of the spatial and temporal scales of denv transmission between mosquitoes and humans. here, we have systematically monitored the circulation of denv in individual aedes spp. mosquitoes and human patients from caratinga, a dengue endemic city in th ...201829394906
natural vertical transmission of dengue virus in aedes aegypti and aedes albopictus: a systematic review.dengue is of great concern in various parts of the world, especially in tropical and subtropical countries where the mosquito vectors aedes aegypti and aedes albopictus are present. the transmission of this virus to humans, by what is known as horizontal transmission, occurs through the bite of infected females of one or other of the two mosquito species. furthermore, an infected female or male parent, by what is known as vertical transmission, can transfer this arbovirus to some part of their o ...201829391071
altered vector competence in an experimental mosquito-mouse transmission model of zika infection.few animal models of zika virus (zikv) infection have incorporated arthropod-borne transmission. here, we establish an aedes aegypti mosquito model of zikv infection of mice, and demonstrate altered vector competency among three strains, (orlando, orl, ho chi minh, hcm, and patilas, pat). all strains acquired zikv in their midguts after a blood meal from infected mice, but zikv transmission only occurred in mice fed upon by hcm, and to a lesser extent pat, but not orl, mosquitoes. this defect in ...201829505571
effects of climate change on the spread of zika virus: a public health threat.zika is a vector-borne viral disease transmitted to humans primarily by aedes aegypti mosquitoes. the increased climate instability has contributed to the emergence of infections carried by mosquitoes like dengue, chikungunya and zika. while infection with the zika virus is not new, the recent epidemic of microcephaly in brazil and other countries in south america resulting from the infection of pregnant women with the zika virus raise a number of serious public health concerns. these include th ...201829500926
wolbachia-mediated virus blocking in mosquito cells is dependent on xrn1-mediated viral rna degradation and influenced by viral replication rate.wolbachia is currently being developed as a novel tool to block the transmission of dengue viruses (denv) by aedes aegypti. a number of mechanisms have been proposed to explain the denv-blocking phenotype in mosquitoes, including competition for fatty acids like cholesterol, manipulation of host mirnas and upregulation of innate immune pathways in the mosquito. we examined the various stages in the denv infection process to better understand the mechanism of wolbachia-mediated virus blocking (wm ...201829494679
first evidence of vertical infection of dengue virus 2 in aedes aegypti mosquitoes from sinaloa, mexico.fourteen pools of aedes aegypti larvae collected within the urban area of culiacán, sinaloa, were analyzed by rt-pcr. the results demonstrate, for the first time, the vertical infection of serotype-2 dengue virus (denv-2) in sinaloa, mexico, suggesting that ae. aegypti acts as a natural reservoir of denv-2 in this region.201829485942
hypericum japonicum: a double-headed sword to combat vector control and cancer.mosquito control with naturally derived herbal insecticides has gained much momentum, with the increased insecticide resistance of vectors and the multiple infectious diseases spread by them. yet, recent studies also suggest that mosquitoes could probably transmit some cancerous cells or cancer-causing viruses from one individual to another between their blood meals. the current research thus focused on the screening and characterization of novel plants with both mosquitocidal and anticancerous ...201829476319
in tune with nature: wolbachia does not prevent pre-copula acoustic communication in aedes aegypti.mosquito-borne diseases are rapidly spreading to vast territories, putting at risk most of the world's population. a key player in this scenario is aedes aegypti, a hematophagous species which hosts and transmits viruses causing dengue and other serious illnesses. since vector control strategies relying only on insecticides have proven unsustainable, an alternative method involving the release of wolbachia-harboring individuals has emerged. its successful implementation vastly depends on how fit ...201829471864
dengue viruses and promising envelope protein domain iii-based vaccines.dengue viruses are emerging mosquito-borne pathogens belonging to flaviviridae family which are transmitted to humans via the bites of infected mosquitoes aedes aegypti and aedes albopictus. because of the wide distribution of these mosquito vectors, more than 2.5 billion people are approximately at risk of dengue infection. dengue viruses cause dengue fever and severe life-threatening illnesses as well as dengue hemorrhagic fever and dengue shock syndrome. all four serotypes of dengue virus can ...201829470620
using adult aedes aegypti females to predict areas at risk for dengue transmission: a spatial case-control study.traditional indices for measuring dengue fever risk in a given area are based on the immature forms of the vector (larvae and pupae surveys). however, this is inefficient because only adult female mosquitoes actually transmit the virus. based on these assumptions, our objective was to evaluate the association between an entomological index obtained from adult mosquito traps and the occurrence of dengue in a hyperendemic area. additionally, we compared its cost to that of the breteau index (bi).201829462598
seasonal distribution and container preference ratio of the dengue fever vector (aedes aegypti, diptera: culicidae) in rawalpindi, pakistan.aedes aegypti (l.) and aedes albopictus (skuse) are known vectors of dengue, chikungunya, and other pathogens; however, their ecology and role in virus transmission has not been well studied in pakistan. here, we report on an intensive survey of potential breeding sites of ae. aegypti in rawalpindi, punjab province, pakistan. the study continued for 11 mo and was divided into three seasons: january to june (pre-monsoon), july to september (monsoon), and october-november (post-monsoon). larval mo ...201829462424
improving vector-borne pathogen surveillance: a laboratory-based study exploring the potential to detect dengue virus and malaria parasites in mosquito saliva.vector-borne pathogen surveillance programmes typically rely on the collection of large numbers of potential vectors followed by screening protocols focused on detecting pathogens in the arthropods. these processes are laborious, time consuming, expensive, and require screening of large numbers of samples. to streamline the surveillance process, increase sample throughput, and improve cost-effectiveness, a method to detect dengue virus and malaria parasites (plasmodium falciparum) by leveraging ...201829460859
entomological and epidemiological aspects of dengue epidemics in fortaleza, ceará, brazil, 2001-2012.to characterize the entomological and epidemiological aspects of dengue epidemics occurred in fortaleza, ceará, brazil, from 2001 to 2012.201829451615
dynamic remodeling of lipids coincides with dengue virus replication in the midgut of aedes aegypti mosquitoes.we describe the first comprehensive analysis of the midgut metabolome of aedes aegypti, the primary mosquito vector for arboviruses such as dengue, zika, chikungunya and yellow fever viruses. transmission of these viruses depends on their ability to infect, replicate and disseminate from several tissues in the mosquito vector. the metabolic environments within these tissues play crucial roles in these processes. since these viruses are enveloped, viral replication, assembly and release occur on ...201829447265
mosquitoes of northwestern uganda.despite evidence of arbovirus activity in northwestern uganda (west nile sub-region), there is very limited information on the mosquito fauna of this region. the only published study reported 52 mosquito species in northwestern uganda but this study took place in 1950 and the information has not been updated for more than 60 yr. in january and june 2011, co2 baited-light traps were used to collect 49,231 mosquitoes from four different locations, paraa (9,487), chobe (20,025), sunguru (759), and ...201829444287
the impact of insecticide applications on the dynamics of resistance: the case of four aedes aegypti populations from different brazilian regions.in the tropics, the utilization of insecticides is still an important strategy for controlling aedes aegypti, the principle vector of dengue, chikungunya and zika viruses. however, increasing insecticide resistance in ae. aegypti populations might hinder insecticide efficacy on a long-term basis. it will be important to understand the dynamics and evolution of insecticide resistance by assessing its frequency and the mechanisms by which it occurs.201829432488
optimal control approach for establishing wmelpop wolbachia infection among wild aedes aegypti populations.wolbachia-based biocontrol has recently emerged as a potential method for prevention and control of dengue and other vector-borne diseases. major vector species, such as aedes aegypti females, when deliberately infected with wolbachia become less capable of getting viral infections and transmitting the virus to human hosts. in this paper, we propose an explicit sex-structured population model that describes an interaction of uninfected (wild) male and female mosquitoes and those deliberately inf ...201829429122
a natural agonist of mosquito trpa1 from the medicinal plant cinnamosma fragrans that is toxic, antifeedant, and repellent to the yellow fever mosquito aedes aegypti.plants produce various secondary metabolites that offer a potential source of novel insecticides and repellents for the control of mosquito vectors. plants of the genus cinnamosma are endemic to, and widely-distributed throughout, the island of madagascar. the barks of these species are commonly used in traditional medicines for treating a wide range of maladies. the therapeutic nature of the bark is thought to be associated with its enrichment of pungent drimane sesquiterpenes, which elicit ant ...201829425195
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