Publications

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characterization of the clinical and anatomical pathological changes associated with hepatozoon mocassini infections in unnatural reptilian hosts.laboratory-reared aedes aegypti mosquitoes were employed in the successful transmission of hepatozoon mocassini from a cotton-mouth moccasin (agkistrodon piscivorus leucostoma) to 3 lizard species (sceloporus undulatus, eumeces obsoletus and sceloporus poinsetti). marked to severe lethargy and anorexia developed in the s. undulatus, e. obsoletus and s. poinsetti at 15, 38, and 96 days postinfection (pi), respectively. all 3 lizards developed a leukocytosis and had increased plasma aspartate amin ...19968690537
yellowfever mosquito (diptera:culicidae) introduced into landi kotal, pakistan, by tire importation.although the recent distribution of yellowfever mosquito, aedes aegypti (l.), in pakistan has been restricted to the port city of karachi, adult and immature mosquitoes breeding in imported tires in warehouses at landi kotal (north-west frontier province) were identified as ae. aegypti. the patterns of tire trade and the current disjunct distribution of ae. aegypti indicated that the introduction into landi kotal may have been either from karachi or india. thermal fog application of pirimiphos-m ...19968699469
evaluation of controlled-release mosquito repellent formulations.eight polymer and 9 microcapsule formulations of deet were tested on laboratory rabbits against aedes aegypti and anopheles albimanus. several formulations were significantly more effective than simple (unformulated) deet at the same strength for periods up to 24 h. best results were obtained with a polymer formulation containing a high molecular weight fatty acid and 3 microcapsule formulations containing lanolin, gum arabic, gelatin, tannic acid, stearic acid, polypropylene glycol, water, and ...19968723256
evaluation of the laboratory rabbit model for screening topical mosquito repellents.the laboratory rabbit was evaluated as a model for screening topical mosquito repellents, using data obtained in tests of deet (n,n-diethyl-3-methylbenzamide) against aedes aegypti on humans and rabbits. host-specific differences in the action of the test material were quantified by multiple regression analysis. the test material was less effective but more persistent in tests on rabbits, and responses of the mosquito test population were more variable.19968723273
lipid transfer from insect fat body to lipophorin: comparison between a mosquito triacylglycerol-rich lipophorin and a sphinx moth diacylglycerol-rich lipophorin.two insect lipoproteins, triacylglycerol-rich aedes aegypti lipophorin and diacylglycerol-rich manduca sexta lipophorin, were compared in their ability to load neutral lipid from fat body. when fat body of m. sexta was incubated in vitro with [3h]oleic acid, all radiolabeled fatty acids were esterified, predominantly to triacylglycerol. in a. aegypti fat body, however, half of the label remained as free fatty acids. when a. aegypti fat body was radiolabeled with [3h]glycerol, most of the radiola ...19968725165
ovulation and post-ovulation changes in the terminal region of the ovarioles of the mosquito aedes aegypti (l.) (diptera: culicidae).detachment of the follicular epithelium from the chorion occurs prior to the passage of the mature egg into the calyx lumen, resulting in a mature oocyte lying free in an epithelial sac. the mature oocytes rupture the epithelial sac, including the tunica propria at the posterior pole, then break through the basal (or granular basal) bodies to enter the calyx lumen. just after ovulation, the formerly terminal parts of the ovariole remain attached to the posterior end of the egg sac near its openi ...19968729630
quiescent ovarioles in the mosquito aedes aegypti (l.) (diptera: culicidae). 19968729634
community involvement in the control of aedes aegypti.in the past 15 years, there has been a dramatic resurgence of dengue and dengue hemorrhagic fever worldwide, with increased frequency of epidemics and geographic expansion of both the mosquito vectors and the viruses. the reasons for this resurgence are not well understood, but include uncontrolled and unplanned urbanization, increased movement of people and viruses by airplane and lack of effective control of aedes aegypti, the principal mosquito vector of dengue viruses. the recommended method ...19968740894
surveillance of the mosquito aedes aegypti and its biocontrol with the copepod mesocyclops aspericornis in australian wells and gold mines.a survey of the dengue vector mosquito aedes aegypti was undertaken using funnel traps to detect immature stages (larvae and pupae) in flooded disused mine shafts and wells in charters towers, queensland, northern australia. the town has a history of dengue fever since 1885 when goldminers were the first recorded victims. during the latest dengue epidemic in 1993, 2% of the population had laboratory-confirmed dengue virus type 2, despite source reduction of ae.aegypti breeding-sites at ground le ...19968744708
a placebo controlled clinical trial investigating the efficacy of a homeopathic after-bite gel in reducing mosquito bite induced erythema.a randomised, placebo controlled clinical trial was conducted to examine the efficacy of a homeopathic after-bite gel in the symptomatic relief of mosquito bites. sixty eight healthy volunteers were bitten under laboratory conditions by aedes aegypti mosquitoes at three spots, on the ventral aspect of the forearm. one bite was treated with the homeopathic after-bite gel, another bite with a placebo gel which was identical in appearance and smell to the homeopathic after-bite gel, and the third b ...19958751030
activity of novel juvenoids on arthropods of veterinary importance.different juvenile hormone mimics were compared regarding their efficacy against larval and pupal stages of the diptera aedes aegypti, musca domestica, and lucilia cuprina as well as the cat flea ctenocephalides felis. structure-activity relationship of a series of new diphenylethers was analyzed and compared to juvenoids like methoprene, hydroprene, and fenoxycarb. selected compounds were taken for a competition binding assay and their effect on jh-degradation by esterases was examined. the res ...19968756312
epidemic dengue 2 in the city of djibouti 1991-1992.from october 1991 to february 1992, an outbreak of acute fever (in which thick blood films were negative for malaria) spread rapidly in the city of djibouti, djibouti republic, affecting all age groups and both nationals and foreigners. the estimated number of cases was 12,000. the clinical features were consistent with a non-haemorrhagic dengue-like illness. serum samples from 91 patients were analysed serologically for flavivirus infection (dengue 1-4, west nile, yellow fever, zika, banzi, and ...19968758061
monoclonal antibodies against manduca sexta hemocytes bind aedes aegypti hemocytes: characterization of six monoclonal antibodies that bind hemocytes from both species.the mechanisms by which hemocytes mediate a mosquito's defense response to parasites or pathogens are not well understood. this is due in part to difficulty in collecting intact mosquito hemocytes for experiments and to a lack of reagents, such as antibodies. our objectives were to collect adult aedes aegypti hemocytes under conditions suitable for immunofluorescence microscopy, and to test whether monoclonal antibodies, generated against larval manduca sexta hemocytes, bind adult ae. aegypti he ...19958773196
[human diffusion of arthropod disease vectors throughout the world].the present distribution of animals and plants throughout the world is the result of an evolutionary process involving tectonic, climatic and biotic factors. humans, since their appearance, have contributed to the spreading of many species including disease vectors and pests. when humans left their native african home, they brought with them ectoparasites such as lice and acarids. during the neolithic era, humans were leading domesticated animals which carried their own parasites into new areas. ...19958777543
laboratory evaluation of toxic baits for mosquito (aedes aegypti) larvae.toxic bait discs were prepared by incorporating the larvicide abate (temephos) technical, with yeast and dog biscuit (1:1), the latter forming the attractant source. these were assessed in 1, 10 and 25 litre aquaria and 100 litre drums against aedes aegypti larvae. floating as well as sinking bait discs were found equally effective in controlling free moving larvae. larvae confined in artificially suspended cages were not affected. the baits were found effective for 5 to 6 weeks. toxic disc bait ...19968781040
molecular characterization of the mosquito vitellogenin receptor reveals unexpected high homology to the drosophila yolk protein receptor.the mosquito (aedes aegypti) vitellogenin receptor (aavgr) is a large membrane-bound protein (214 kda when linearized) that mediates internalization of vitellogenin, the major yolk-protein precursor, by oocytes during egg development. we have cloned and sequenced two cdna fragments encompassing the entire coding region of aavgr mrna, to our knowledge the first insect vgr sequence to be reported. the 7.3-kb aavgr mrna is present only in female germ-line cells and is abundant in previtellogenic oo ...19968799131
cloning and expression analysis of aedes aegypti opsin: adaptation of an in situ hybridization protocol for mosquitoes.opsin is a g protein coupled photoreceptor that activates a signal transduction cascade in the ommatidia. its primary and secondary structure is conserved both in insects and vertebrates as exemplified by the drosophila opsins. through serendiplious cloning of a pcr fragment, we have identified an opsin cdna. the latter was used to clone full length cdnas from a mosquito head library. the main purpose of cloning was to have a positive control probe to establish an in situ hybridization protocol ...19968799735
effect of cetirizine on the inflammatory cells in mosquito bites.mosquito bites frequently cause wealing and delayed papules which appear within a few hours after the bites and may persist for several days. cetirizine is an effective drug against mosquito bites by decreasing wealing and also the size and pruritus of the delayed bite papules.19968809428
bioefficacy of mosquito mat, coil and dispenser formulations containing allethrin group of synthetic pyrethroids against mosquito vectors.the bioefficacy of mats, coils and dispensers containing allethrin group of synthetic pyrethroids was studied against laboratory strains of culex quinquefasciatus, aedes aegypti and anopheles stephensi. except esbiothrin in the mat formulation which was 100 and 178 times more effective against cx. quinquefasciatus than against ae. aegypti and an. stephensi with kt50 of 0.005, 0.5 and 0.89 min. respectively, all the three allethrins in mat or coil formulations were equally effective against the t ...19968810142
effect of inactivation by sunlight on the larvicidal activities of mosquitocidal bacillus thuringiensis h-14 isolates from nigerian soils.bacillus thuringiensis h-14 is a well recognized bioinsecticide against mosquitoes. this study investigates the inactivation effect of sunlight on the larvicidal activities of four nigerian isolates of b. thuringiensis h-14, code-named obg1, obg8, gsc3 and gna13 as compared with a standard mosquito larvicide, b. thuringiensis var. israelensis (bti). a 0.125 mg/1 suspension of spore-delta-endotoxin powder of each isolate was exposed to direct sunlight for 7 h on a concrete roof (ca. 13 m high). a ...19968810143
evaluation of bacillus thuringiensis and bacillus sphaericus strains from chinese soils toxic to mosquito larvaeduring our research to isolate and screen microbial agents, eight bacillus thuringiensis isolates and five bacillus sphaericus isolates were shown to have high toxicity to mosquito larvae. comparing the lc50 values, four b. sphaericus isolates with lc50 values ranging from 0.50 to 1.47 ng/ml were about two to six times more toxic than strain 1593 (lc50 3.00 ng/ml) against larvae of culex quinquefasciatus. four b. thuringiensis isolates (lc50 values ranging from 3.80 to 7.54 ng/ml) and four b. sp ...19968812574
cloning and characterization of cdnas encoding the antibacterial peptide, defensin a, from the mosquito, aedes aegypti.insect defensins are cationic, inducible antibacterial peptides. four full-length cdnas encoding defensin a from the mosquito aedes aegypti were cloned using polymerase chain reaction (pcr) and sequenced. all four cdnas are 473 base pairs long, bearing an open reading frame of 98 amino acids with a few substitutions in the signal peptide domain. the deduced amino acid sequence of aedes aegypti defensin (aadef) contains a signal peptide sequence of 18 amino acids followed by a 40-amino acid putat ...19968814787
larvicidal constituents of melantheria albinervia.bioactivity-guided fractionation has led to the isolation of two larvicidal diterpenes, active against the yellow fever-transmitting mosquito aedes aegypti, from a dichloromethane root extract of melantheria albinervia (asteraceae), a plant from zimbabwe. these diterpenes were identified as ent-kaur-16-en-19-oic acid and 9(11),16-kauradien-19-oic acid. the diterpenes were also weakly active against bacillus subtilis.19958824954
survey of container-inhabiting mosquitoes in clemson, south carolina, with emphasis on aedes albopictus.a total of 530 oviposition trap samples were collected within a 10-km radius of clemson university between march 30 and october 19, 1993. from 19,664 larvae reared from collected eggs, 7 species were identified: aedes albopictus (89%), ae. triseriatus (6.5%), culex restuans (2.7%), cx. territans (0.6%), cx. pipiens complex (0.7%), toxorhynchites rutilus septentrionalis (0.2%), and orthopodomyia signifera (0.1%). this is the first record of ae. albopictus in clemson. aedes aegypti was not found. ...19958825496
country-wide prevalence of ascogregarina culicis (apicomplexa: lecudinidae), a protozoan parasite of aedes aegypti in trinidad, west indies.the prevalence of the gregarine parasite ascogregarina culicis was determined for aedes aegypti populations in trinidad, west indies. over 5 months, a total of 5,651 ae. aegypti larvae were collected and identified from 419 sites throughout trinidad as part of a national dengue surveillance program. ascogregarina culicis infections were detected in 13.4% of 1,986 ae. aegypti larvae dissected. in 7 of 8 counties or major subdivisions where this parasite was detected in trinidad, the prevalence pe ...19958825500
genetic differentiation of aedes aegypti, the vector of dengue virus in french polynesia.in recent years the incidence of dengue fever epidemics has increased and transmission has tended to be established over a geographically expanding area, including french polynesia. an increase in air transportation contributes to the diffusion of the dengue virus from southeast asia, a region considered to be a hyperendemic dengue zone, to the pacific region. presently, little is known about the role of the vector (aedes aegypti) in the diffusion of the dengue fever virus. a study on the geneti ...19958825508
a new serovar of bacillus thuringiensis possessing 28a28c flagellar antigenic structure: bacillus thuringiensis serovar jegathesan, selectively toxic against mosquito larvae.a novel bacillus thuringiensis strain highly toxic to mosquitoes was isolated from soil samples in malaysia. this strain was shown to display a new subfraction of the h-28 flagellar antigen determining a new serovar h28a28c, which was designated serovar jegathesan. bioassays indicated that culex quinquefasciatus larvae are the most susceptible to this new isolate, whereas toxicity to anopheles maculatus and aedes aegypti larvae was 10 times lower. the potency of this new serotype is also compara ...19958825511
evaluation of entomopathogenic bacteria against aedes polynesiensis, the vector of lymphatic filariasis in french polynesia.thirteen strains among 3 species of entomopathogenic bacteria were tested against 3 medically important mosquito species in french polynesia. two strains of bacillus thuringiensis were highly toxic to aedes polynesiensis, aedes aegypti, and culex quinquefasciatus. six of 7 strains of bacillus sphaericus tested were highly toxic to cx. quinquefasciatus but not to the aedes spp. clostridium bifermentans serovar. malaysia was more toxic to ae. polynesiensis than to the other 2 species. entomopathog ...19958825516
community based dengue control in columbia: people's knowledge and practice and the potential contribution of the biological larvicide bti (bacillus thuringiensis israelensis).a study on dengue control was undertaken in a poor urban area in cúcuta, colombia. the first objective was to describe people's knowledge, attitudes and practices regarding dengue fever, the transmission of the disease and possible preventive measures. the second objective was to analyse the infestation of the community with aedes aegypti larvae, and the third objective to test the efficacy of bti (bacillus thuringiensis israelensis) with respect to the level and duration of reduction of ae. aeg ...19958826104
laboratory and field plot bioassay of bacillus sphaericus against arkansas mosquito species.bacillus sphaericus was evaluated in laboratory bioassays against larvae of 7 arkansas mosquito species and in rice field plots against a single mosquito species. laboratory-tested species included aedes aegypti, ae. albopictus, ae. vexans, anopheles punctipennis, culex quinquefasciatus, cx. restuans, and psorophora columbiae. field plot bioassays were evaluated against ps. columbiae only. the laboratory formulation tested demonstrated activity against all 7 species. mortalities were observed at ...19968827596
response of anopheles farauti to permethrin-treated net and cloth fabrics in the laboratory.the median effective dose of permethrin for knockdown and mortality of anopheles farauti s. s. exposed to 2 net and 2 cloth fabrics was estimated. there were no significant differences in the knockdown or mortality of an. farauti or aedes aegypti following 15-180-sec exposure to permethrin-treated bed nets and polyester/cotton fabric. despite the presence of permethrin (0.007-0.068 mg/cm2) in bed net material, 18.2-50% of an. farauti adults successfully obtained a blood meal through the nets, an ...19968827613
stability of equine infectious anemia virus in aedes aegypti (diptera: culicidae), stomoxys calcitrans (diptera:muscidae), and tabanus fuscicostatus (diptera:tabanidae) stored at -70 degrees c.equine infectious anemia virus (eiav) was injected intrathoracically into aedes aegypti, stomoxys calcitrans, and tabanus fuscicostatus, and fed to ae. aegypti in suspensions of either artificial blood of eagle's minimum essential medium. insects were stored at -70 degrees c for up to 9 months before testing for the presence of eiav. the viral tissue culture titers detected from stored insects were similar to those from insects tested at time 0.19968827617
yellow fever: a decade of reemergence.since the 1980s, yellow fever has reemerged across africa and in south america. the total of 18 735 yellow fever cases and 4522 deaths reported from 1987 to 1991 represents the greatest amount of yellow fever activity reported to the world health organization (who) for any 5-year period since 1948. there is an excellent vaccine against yellow fever. at present, a high proportion of travelers to at-risk areas are reported to be immunized, reflecting widespread knowledge about the international he ...19968827969
dengue 3 virus distribution in the mosquito aedes aegypti: an immunocytochemical study.the dissemination of dengue (den) 3 virus in parenterally infected female aedes aegypti mosquitoes was studied immunocytochemically. antigen was first detected in fat body cells near the thoracic site of virus inoculation. the intussuscepted foregut, salivary glands and nervous tissue were the first major tissues infected. nervous tissue appeared to be the primary site of amplification. muscles, tracheae, malphigian tubules and the posterior midgut did not become infected. the only part of the r ...19968834747
entomological studies in a dengue endemic area, jalore, rajasthan.entomological studies on prevalence of adult and immatures of aedes aegypti along with associated ecological factors have been conducted in a dengue endemic area of jalore, rajasthan from 1992 to 1993. studies in two areas; reportedly affected and unaffected revealed more adults and higher breeding indices in the affected area as compared to the unaffected one. mosquitoes harbouring dengue antigen were found only in the affected locality. presence of dengue antigen in field caught mosquitoes in ...19968840653
enzymatic variability in natural populations of aedes aegypti (diptera:culicidae) from brazil.yellowfever mosquitoes, aedes aegypti (l), were collected from 3 towns located within 70 km of each other in the state of sao paulo to evaluate the protein variability of natural populations. electrophoretic analysis permitted the identification of 6 loci responsible for the production of leucine aminopeptidase, 3 for esterases and malic enzyme, 2 for malate dehydrogenase, isocitrate dehydrogenase and alpha-glycerophosphate dehydrogenase, and 1 for phosphoglucomutase. for the 3 populations, the ...19968840678
intensive linkage mapping in a wasp (bracon hebetor) and a mosquito (aedes aegypti) with single-strand conformation polymorphism analysis of random amplified polymorphic dna markers.the use of random amplified polymorphic dna from the polymerase chain reaction (rapd-pcr) allows efficient construction of saturated linkage maps. however, when analyzed by agarose gel electrophoresis, most rapd-pcr markers segregate as dominant alleles, reducing the amount of linkage information obtained. we describe the use of single strand conformation polymorphism (sscp) analysis of rapd markers to generate linkage maps in a haplodiploid parasitic wasp bracon (habrobracon) hebetor and a dipl ...08844159
[yellow fever in the ribeirão preto region at the turn of the 19th century: its scientific importance and economic repercussions].yellow fever in the region of ribeirao preto at the turn of xix century: scientific importance and economic repercussion. this historical review describes the bad situation of public health in brazil during the xix century caused by multiple yellow fever outbreaks. the knowledge regarding to yellow fever at that time is also described. a short history is presented of the development of the region of ribeirao preto, located in the northeast of sao paulo state, brazil, emphasising the actuation of ...19968851220
generation of expressed sequence tags and sequence-tagged sites as physical landmarks in the mosquito, aedes aegypti, genome.twenty-three clones were developed as expressed sequence tags (ests) and sequence-tagged sites (stss) that provide broad coverage of the mosquito, aedes aegypti, genome at an average spacing of about 7.2 cm. each of these clones had been mapped previously as a restriction fragment length polymorphism (rflp) marker. nineteen of these clones represent anonymous cdnas and 4 clones represent known genes. forty-seven percent of the anonymous cdnas showed significant deduced amino acid sequence simila ...19968851807
cell targeting of a pore-forming toxin, cyta delta-endotoxin from bacillus thuringiensis subspecies israelensis, by conjugating cyta with anti-thy 1 monoclonal antibodies and insulin.the cytolytic protein toxin cyta was linked to two monoclonal antibodies (mab) directed against the mouse or the rat thy 1 antigen. the purified cyta-mab conjugates were not toxic to either target or nontarget cells. the conjugates did bind specifically to target cells since they agglutinated the target cells but not nontarget cells. when the conjugates were treated with dithiothreitol, the released cyta was toxic to all cells tested. these results suggested that the attachment of cyta to a mole ...19968853459
acasp, a gene encoding a cathepsin d-like aspartic protease from the hookworm ancylostoma caninum.intestinal infection with the zoonotic hookworm ancylostoma caninum can provoke human eosinophilic enteritis. a cdna was isolated from a. caninum, using an oligonucleotide primer designed to hybridize to the region encoding the consensus, catalytic site residues d32tgssnlw of aspartic proteases. this novel cdna encoded an aspartic protease zymogen of 422 amino acids, exhibiting 47% identity to the lysosomal aspartic protease of aedes aegypti, 46% identity to the aspartic protease of schistosoma ...19968858139
phenotypic segregation of aedes aegypti for immune antibacterial activity and resistance to filariae.we report the phenotypic selection of two lines of aedes aegypti, from a filariae susceptible parental stock (refm). this selection was based upon the level of inducible anti-gram negative escherichia coli activity within the haemolymph following e. coli infection. these lines, denoted 'high' and 'low', demonstrated significant differences in anti-e. coli responses throughout. however no difference was observed in the anti-gram positive micrococcus luteus response following e. coli challenge. f4 ...19968858873
the efficacy of prrrikweg gel in the treatment of insect bites: a double-blind, placebo-controlled clinical trial.this study was conducted to examine the efficacy of prrikweg gel, a homeopathic after-bite gel, in relieving the effects of mosquito bites, in particular itching and erythema.19968861830
setaria digitata microfilaraemia in mastomys coucha: an animal model for chemotherapeutic and immunobiological studies.intraperitoneal implantation of adult gravid females of the bovine filarial parasite, setaria digitata in mastomys coucha was found to induce microfilaraemia lasting for about 125 days. the microfilariae (mf) could be detected as early as 4 days post-implantation (p.i.) and peak levels of about 30 mf in 20 microliters of blood were observed by 21 days. a significant positive correlation was found between mf density and the body weight of recipients pre-implantation. the implanted adult worms wer ...19968873474
early trypsin, a female-specific midgut protease in aedes aegypti: isolation, aminoterminal sequence determination, and cloning and sequencing of the gene.early trypsin is a female-specific protease present in the aedes aegypti midgut during the first hours after ingestion of a blood meal. it plays an essential role in the transcriptional activation of the late trypsin form, the major midgut endoprotease involved in the blood meal digestion. early trypsin is the most abundant midgut polypeptide isolated by benzamidine-sepharose affinity chromatography 3 h after feeding. the amino-terminal sequence of the early trypsin protein matches that of the 3 ...19968882654
mosquito vector control and biology in latin america--a sixth symposium.the sixth spanish language symposium presented by the american mosquito control association (amca) was held as part of the 62nd annual meeting in norfolk, va, in march 1996. the principal objective, as for the previous 5 symposia, was to promote the participation in the amca meeting by vector control specialists, public health workers, and academicians from latin america. this publication includes summaries of 25 presentations that were given in spanish by participants from 6 countries in latin ...19968887226
insecticide susceptibility of aedes aegypti and aedes albopictus in the lower rio grande valley of texas and mexico.in response to a potential dengue fever outbreak in south texas during 1995, the susceptibilities of aedes aegypti and aedes albopictus to commonly used mosquito adulticides were assessed. larvae collected from the lower rio grande valley of texas and mexico were reared to adults and tested against susceptible laboratory strains at texas a&m university. resistance ratios at both the lc50 and lc95 rates were all less than 10, indicating that adult populations of both species are still susceptible ...19968887230
discovery of aedes albopictus in guatemala.aedes albopictus was discovered for the first time in guatemala during june 1995. it was collected in 10 out of 15 localities sampled in and around the community of puerto barrios in the department of izabal. the location of the positive collections indicates a more rural distribution than aedes aegypti. sampling was conducted along the atlantic highway up to the capital city to determine the extent of its introduction into the country. larvae were not found more than 4 km outside of puerto barr ...19968887235
effects of variations in a formulated protein meal on the fecundity and fertility of female mosquitoes.a formulated protein meal developed by kogan (1990) for adult female aedes aegypti mosquitoes was evaluated and modified to increase egg and pupal yield. a vigorous laboratory colony was maintained with the females fed exclusively on this dietary formula for about twenty-five generations over more than 2 years. extra modifications were made to produce a diet suitable for anopheles arabiensis and anopheles stephensi females to produce eggs. both formulations contain bovine albumin, haemoglobin an ...19968887337
adaptive larval thermotolerance and induced cross-tolerance to propoxur insecticide in mosquitoes anopheles stephensi and aedes aegypti.fourth-instar larvae of mosquitoes anopheles stephensi and aedes aegypti normally died within 90 min at 43 degrees c. pre-exposure to high but sublethal temperatures conferred adaptive thermotolerance, dependent on the temperature and the duration of pre-exposure. adaptive cross-tolerance to propoxur (a carbamate insecticide) was also induced in larvae by pre-exposing them to sublethal temperatures. pre-exposure to sublethal concentrations of propoxur was found to confer cross-thermotolerance to ...19968887340
heritability of infection-induced humoral antibacterial activity in the immune response of the mosquito aedes aegypti. 19968887345
the mosquito ultraspiracle homologue, a partner of ecdysteroid receptor heterodimer: cloning and characterization of isoforms expressed during vitellogenesis.we report the cloning and characterization of two isoforms of the ultraspiracle homologue (aausp) from the mosquito, aedes aegypti. the 2.33-kb aauspa cdna has an open reading frame (orf) of 484 amino acids encoding a polypeptide of 54 kda, whereas the 2.14-kb aauspb orf of 459 amino acids encodes a 51.3 kda polypeptide. the aauspa and aauspb proteins differ only in the n-terminal portion of the variable a/b domain. the aausp dna-binding domain shares 92% and 97% identities with the respective d ...19968892313
a search for components in human body odour that attract females of aedes aegypti.in a new type of wind tunnel, mosquitoes fly upwind towards host odour sources and towards human skin wash extracts obtained by rubbing the skin with a pad soaked in ethanol. we used this behavioural response as a bioassay to identify attractants in liquid chromatography fractions of such extracts. l-lactic acid is a major constituent of skin wash extracts and it is a necessary component for the extract's effectiveness. as a single stimulus, however, l-lactic acid is only slightly effective. thi ...19968894295
central olfactory pathways in mosquitoes and other insects.studies of cns processing of olfactory information have contributed significantly to understanding olfactory-guided behaviour in insects. evidence in moths suggests that each glomerulus in the antennal lobes has a unique property: receptor-axon projections and dendritic arborizations of uniglomerular output neurons can relate to anatomically and functionally distinct glomeruli. similar correlations are not typical of the concentrically organized locust antennal lobes. insights about odour proces ...19968894298
sensory aspects of host location in mosquitoes.visual, thermal and olfactory stimuli all contribute to blood meal host location in mosquitoes but olfaction is probably the dominant sensory modality used for this purpose. much attention has been devoted to the l-lactic acid receptor because it is well characterized and because its sensitivity is a major determinant of host responsiveness in the anautogenous species aedes aegypti. studies employing statistical analysis of close to 500 single unit recordings and the scanning electron microscope ...19968894299
responses of antennal olfactory receptors in the yellow fever mosquito aedes aegypti to human body odours.recent behavioural studies have demonstrated that human body odours which female aedes aegypti find attractive exert their effects as complex mixtures of synergistically acting components. we have attempted to clarify the sensory mechanisms underlying the perception of these complex host odours by studying the responses of sensory cells underneath the a3-type sensilla of the mosquito antenna to both a human skin wash extract and the extract's active chromatographic fractions. the reaction patter ...19968894302
purification and characterization of a major glutathione s-transferase from the mosquito anopheles dirus (species b).the major form of glutathione s-transferase (gst) activity from the mosquito anopheles dirus (species b), a vector of malaria in thailand has been purified 421-fold. it constituted approx. 20% of the total measured cdnb conjugating activity in the homogenate. this enzyme appeared as a single band of 25.0 +/- 0.26 kda on sds-page and was kinetically characterized with 10 substrates and 4 inhibitors. the enzyme is capable of catalysing dehydrochlorination of 1,1,1-trichloro-2,2-bis-(p-chlorophenyl ...19968900597
involvement of peroxidase in chorion hardening in aedes aegypti.peroxidase activity is detectable in aedes aegypti ovaries, containing developing eggs, at 24 h following blood feeding, and peak peroxidase activity is reached at 36-48 h after the blood-meal. peroxidase is associated with the chorion layer in mature eggs and the majority of the enzyme is released from the chorion layer by treating the isolated chorion fraction with sds/urea. analysis of the sds/urea solubilized chorion proteins using sds-page with tropolone/h2o2 or dopa staining verified the p ...19968900599
inter- and intraspecific variation in resistance to desiccation by adult aedes (stegomyia) spp. (diptera: culicidae) from indonesia.desiccation survival times of adult mosquitoes were studied for yellow fever mosquito, aedes aegypti (l.) (6 strains), asian tiger mosquito, ae. albopictus (skuse) (5 strains), and ae. paullusi stone & farmer (1 strain) colonized from south sulawesi, indonesia. at both 90 and 70% rh, ae. aegypti males and females outlived the other species. the forest species ae. paullusi was least resistant to desiccation. strains of ae. aegypti and ae. albopictus from urban areas were more resistant to desicca ...19968906905
occurrence of aedes (stegomyia) spp. mosquitoes (diptera: culicidae) in halmahela villages, indonesia.aedes (stegomyia) spp. mosquitoes breeding in containers in and around human houses were surveyed in halmahela villages, the north moluccas, indonesia. adults reared from larvae found in containers and those reared from eggs collected by ovitraps revealed the dominance of aedes scutellaris (walker) in residential areas. breeding of aedes aegypti (l.) and aedes albopictus (skuse) was confirmed in a few coastal villages with high human densities.19968906924
attraction and trapping of aedes aegypti (diptera: culicidae) with host odors in the laboratory.volatile constituents of mouse odor were eluted from an adsorbent after collection within a closed-air system, and offered to host-seeking yellowfever mosquitoes, aedes aegypti (l.), in the laboratory. gas chromatographic analysis showed that individual mouse odors varied in blend, both quantitatively and qualitatively. in a 2-choice bioassay, sticky traps baited with individual mouse odors caught more female mosquitoes than controls (69% caught on baited traps) in all cases. when 2 traps baited ...19968906926
identification and characterization of differentially expressed cdnas of the vector mosquito, anopheles gambiae.the isolation and study of anopheles gambiae genes that are differentially expressed in development, notably in tissues associated with the maturation and transmission of the malaria parasite, is important for the elucidation of basic molecular mechanisms underlying vector-parasite interactions. we have used the differential display technique to screen for mrnas specifically expressed in adult males, females, and midgut tissues of blood-fed and unfed females. we also screened for mrnas specifica ...19968917545
[ecology of aedes aegypti in africa and asia].aedes aegypti is originated from africa and expanded around the tropical world with a pantropical distribution in 1930. because of mosquito control, this extension decreased, but is still very important and threatening because of disease transmission. eggs of ae. aegypti can survive unfavorable conditions. larvae and pupae of ae. aegypti breed into natural or artificial containers, the last being the most important. artificial breeding-sites are mostly water storage containers and discarded cont ...19968924766
[the risk of urban yellow fever outbreaks in brazil by dengue vectors. aedes aegypti and aedes albopictus].urban yellow fever (yf) epidemics have disappeared from brazil since about 50 years, but a selvatic cycle still exist. in many states, cases are more or less numerous each year. ae. aegypti was eradicated in 1954, re-appeared temporarily in 1967, and then definitively in 1976-1977. ae. aegypti is a vector of yellow few (yf), but also of dengue, whose first cases were reported in 1982. today, dengue is endemic in many regions. a second flavivirus vector, aedes albopictus is present since about te ...19968924767
[aedes aegypti in french guiana. some aspects of history, general ecology and vertical transmission of the dengue virus].aedes aegypti is suspected to be present in the country since the late 18th century, and was responsible of urban yellow fever epidemics in the last century. this mosquito was identified for the first time in french guiana in 1902. more recently, in 1940, an eradication campaign started and aedes aegypti was eradicated between 1950 and 1963, date of the reinfestation. during the past 30 years, some dengue outbreaks occurred every 2 to 6 years, and the first dengue haemorrhagic fever epidemic spr ...19968924768
[oviposition and dispersion of aedes aegypti in an urban environment].it is generally accepted that female aedes aegypti do not fly more than 50-100 m in their entire lifetime, yet the rapidity with which this species colonizes new areas, and the explosive nature of dengue and yellow fever epidemics appear to contradict this. using molecular methods, we have confirmed that the ae. aegypti females lay small numbers of eggs at many sites. the distribution of available sites implies that the female may fly a considerable distance to deposit her whole egg batch. we de ...19968924769
[prediction and prevention of dengue epidemics].prediction and prevention of dengue epidemics are based on informations gathered about the mosquito vector species, the dengue types transmitted, the vertebrate hosts and their environment. although aedes aegypti is the most important vector, other aedes may also propagate the dengue viruses. the populations of vector mosquitoes are evaluated through several indices: breteau and/or positive house index, number of indoor resting aedes females, etc.... the four dengue types can replicate in verteb ...19968924770
[dengue and its vectors in brazil].history of dengue in brazil is covered from the first citations in the xixth century to the great outbreaks of the last ten years. den-1 and den-4 viruses have been isolated for the first time in 1982 during an epidemic in boa vista, roraima state. in 1986-1987, epidemics of dengue type 1 covered an extended area from rio de janeiro/sào paulo states to the north east states of brazil. during 1990-1991, dengue type 2 epidemics have been notified in the south east (rio de janeiro/são paulo) and in ...19968924771
[the prevention of dengue in guadeloupe].dengue is the only disease transmitted by mosquitoes in the guadeloupe islands of french antilles. two dengue outbreaks were reported in 1992 and 1994. aedes aegypti is recognized the only vector of dengue in guadeloupe, and the 200 l drums are the most important breeding-sites. vector control in guadeloupe includes community health education, larvicide and adulticide spraying, and the destruction of the breeding-sites. however, ae. aegypti control has limitations such as the lack of participati ...19968924773
[control of mosquitos in martinique. a collective public health action and improvement of the quality of life].aedes aegypti control program in martinique started en 1968. the aedes aegypti major breeding sites are associated with flowers vases, drums, used tyres, artificial containers and watering places. however, culex quinquefasciatus is the main nuisance mosquito in all the island. this species is unpleasant, especially in urban areas. over 200 culex quinquefasciatus bites per person per night have been observed in several localities of the island. culex quinquefasciatus breeding sites are mostly ass ...19968924774
[anti-aedes aegypti campaign in french guiana].the history of vector control in french guiana started in 1947 when a small team was recruited for that purpose. in 1949, the first ddt treatments were implemented and aedes aegypti could not be found until 1960. between 1966 and 1972 an eradication campaign was carried out which resulted in the elimination of ae. aegypti from all the department except the city of cayenne. malathion and orthodibrom were used as adulticides and temephos (abate) was sprayed against the larvae. nevertheless, in 198 ...19968924775
[epidemiology of arbovirus diseases: use and value of physiologic age determination of female mosquito vectors].the physiological age of yellow fever aedes females in africa was studied during four years, from 1988 to 1992. we used a method, according to polovodova's method, which looks for the "yellow body" under natural light. those yellow bodies exist in the old females, the "parous" ones, and not in the young females, the "nulliparous" ones. we present some results to illustrate the interest of studying the physiological age of mosquitoes in the epidemiology of the arboviral diseases. the transmission ...19968924776
[control of aedes aegypti in martinique. contribution of entomology studies].the dengue prevention activities in martinique are based on: entomological surveillance (including the use of insecticide in all parts of the island). epidemiological (clinical, serological and virological) surveillance. health education (radio, tv, exhibitions, talks in primaries and secondaries schools, in associations,...). the main breeding sites of aedes aegypti are maintained by human practices: flowers vases, containers, used tyres, waste... the role of those human practices in the standi ...19968924777
[new epidemiological aspects of dengue].since the 1940s, the dengue epidemics occur more and more often in the pacific islands with an increased severity. for example, in new caledonia, outbreaks of dengue-like diseases have been reported since the end of the last century but the first epidemic due to an identified virus occurred in 1942-1943 and was caused by the den-1 type. the next, due to the den-2 type, was reported thirty years later, in 1972-1973. after that, three outbreaks burst between 1975 and 1990, caused successively by d ...19968924778
[the situation of dengue in the world].the situation of dengue in the world can be summarized as follows: 2,5 billions persons at risk, 60 millions cases per year, and 30 000 deaths per year. the four dengue serotypes, den-1, den-2, den-3 and den-4 are nearly human-specific. clinical symptoms of dengue vary from unapparent infection, mild febrile manifestations to hemorrhagic forms with or without shock syndrome. dengue viruses are transmitted by aedes mosquitoes, the most important vectors being aedes aegypti and aedes albopictus. t ...19968924780
[dengue in the americas].dengue fever is endemic from argentina to mexico, where serotypes 1, 2 and 4 are in wide circulation. after an absence of almost 20 years, serotype 3 arrived in central america in late 1994. over the past decade dengue has become a major public health problem in many of these countries, with an increase in the prevalence of dengue haemorrhagic fever and in the number of fatal cases. the situation is not new; there is evidence of a least eight pandemics from the early 19th century until the initi ...19968924781
[dengue in french guiana. history and present status].the evolution of dengue in french guiana has the same trend as in most tropical american countries, with emergence of dengue hemorrhagic fever (dhf) and endemicity of the disease. during the 1940's, several dengue-like outbreaks were reported from french guiana. then, the only dengue vector, aedes aegypti, was eradicated between 1950 and 1963, when it started to reinfest the country. by the end of the 1960's, new dengue epidemics with serological confirmation were described. after 1970, the firs ...19968924782
apyrase and alpha-glucosidase in the salivary glands of aedes albopictus.an apyrase and an alpha-glucosidase were detected in the salivary glands extracts of adult aedes albopictus. the apyrase is a 61,000 da secreted protein that hydrolyses atp and adp. this protein is synthe-sized in adults and is preferentially accumulated in the distal lateral lobes of the female salivary glands. the alpha-glucosidase is a secreted 67,000 da protein. this enzyme is synthesized during adult life and accumulated in the proximal-lateral lobes of both males and females. the results a ...19968925436
dengue fever at the u.s.-mexico border, 1995-1996.dengue is a mosquito-transmitted acute disease caused by any of four virus serotypes (den-1, den-2, den-3 and den-4) and is characterized by acute manifestations that can include fever, headache, myalgia, arthralgia, rash, nausea, and vomiting. on august 25, 1995, public health authorities in mexico notified the texas department of health (tdh) of an ongoing outbreak of dengue fever in the state of tamaulipas, which borders south texas. because of the year-round presence of the aedes aegypti mos ...19968927003
pathogenicity of the aedes albopictus parvovirus (aapv), a denso-like virus, for aedes aegypti mosquitoes.aapv, a denso-like virus isolated from a c6/36 clone of the aedes albopictus cell line, proved to be very pathogenic for aedes aegypti first and third instar larvae following per os infection. the mortality reached 90% in 10 days for larvae infected at the first instar. several factors, such as temperature, larval density and stage, and duration of contact with infectious particles, influenced infection. the virus titer in females surviving infection at the third larval instar reached 10(8) tcid ...19968931366
peritrophic matrix proteins of anopheles gambiae and aedes aegypti.little is known about the composition and function of the mosquito peritrophic matrix (pm), a physical barrier that pathogens must traverse to complete their life cycles. anopheles gambiae and aedes aegypti pm proteins induced by blood or by a protein-free meal have been characterized by the use of 2-d gel electrophoresis and lectin-binding affinity assays. more than forty proteins have been identified in both species. over half of the pm proteins of both mosquitoes migrate identically. many pm ...19968933177
muscle actin gene from aedes aegypti (diptera:culicidae).a recombinant phagemid containing a 1,240-bp insert encoding an actin was isolated from a yellow fever mosquito, aedes aegypti (l.), complementary dna library. this insert (pbs-act35) contained an open reading frame of 822 bp whose deduced amino acid sequence exhibited > 95% homology with the carboxyl terminal 274 amino acids of drosophila melanogaster meigen and silkworm, bombyx mori (l.), actin genes. reverse transcriptase-polymerase chain reaction was used to clone and determine the sequence ...19968961646
sensory bases of attractancy: morphology of mosquito olfactory sensilla-- a review.the internal and external ultrastructure of virtually all of the olfactory sensilla of the mosquitoes aedes aegypti and anopheles stephensi have been described. many other mosquitoes have had their olfactory complements partially described. this review summarizes ultrastructural features and suspected biological roles of the small sensilla coeloconica, large sensilla coeloconica, sensilla ampullaceae, grooved pegs and sensilla trichodea of mosquito antennae, and the capitate pegs of mosquito pal ...19948965084
endogenous regulation of the attraction of aedes aegypti mosquitoes.even when host attractants are present, there are times when endogenous physiological mechanisms prevent female mosquitoes from responding to them and engaging in host-seeking behavior. these times include a brief postemergence period, periods of the day determined by a circadian clock, and following a blood meal before and after eggs develop, which are controlled by nervous and humoral mechanisms. other factors such as age, nutrition, and mating status can further modulate host-seeking behavior ...19948965086
changes in ribosomal protein rpl8 mrna during the reproductive cycle of the mosquito, aedes aegypti.changes in the abundance of ribosomal protein rpl8 mrna were compared with 18s rrna and vitellogenin mrna in fat body from adult female aedes aegypti during the reproductive cycle. levels of rpl8 mrna began to increase within 2 h after adult eclosion, peaked at about 24 h post-eclosion, and remained high throughout the next 48 h. during this same period, rrna abundance increased about 2-fold. after the bloodmeal, levels of rpl8 mrna gradually decreased over the next 48 h, while rrna levels incre ...19968969467
disposable containers as larval habitats for aedes aegypti in a city with regular refuse collection: a study in marília, são paulo state, brazil.in marília, brazil, refuse is collected at least every other day, yet non-useful, non-returnable containers such as cans, plastic bottles and tires account for almost half of the container habitats found positive for the aedes aegypti mosquito. a study was therefore conducted to investigate why these containers exist despite regular refuse collection and a high level of awareness of dengue prevention, and how the control program could most effectively respond. differing community perceptions as ...19968971274
[the yeast flora of blood-sucking mosquitoes].yeast flora composition has been studied in natural populations of blood-sucking mosquitoes of genera aedes. anopheles and culex dwelling in the territory of ukraine. the authors have distinguished 18 yeast species belonging to 7 genera. species candida famata, c. (yarrowia) lipolytica as well as the yeast of genera rhodotorula and cryptococcus dominate on the larvae and imago of mosquitoes. the distinguished strains are the components of normal microflora of blood-sucking mosquitoes and take no ...19968983519
the effect of chitin synthesis inhibitors on the development of brugia malayi in aedes aegypti.two chitin synthesis inhibitors (csis) viz., triflumuron and hexaflumuron interfere++ with the development of brugia malayi in aedes aegypti (a black-eyed liverpool strain). the development of b. malayi was slow in both the treated populations and the infection rate, infectivity rate and l3 load per mosquito decreased significantly (p < 0.001) in comparison with untreated controls. hexaflumuron was found to be more inhibiting than triflumuron.19968984113
in vivo effect of a natural aedes aegypti defensin on brugia pahangi development. 19968994145
nad(p)h-dependent production of oxygen reactive species by the salivary glands of the mosquito anopheles albimanus.salivary gland homogenates of the adult female mosquito anopheles albimanus, but not those of aedes aegypti, induced light production in the presence of nadph and luminol, indicating a nadph oxidase activity producing reactive oxygen species (superoxide anion) by the anopheline salivary homogenate. superoxide production by the anopheline salivary homogenate was also confirmed by the nadph-dependent, superoxide dismutase inhibitable, reduction of cytochrome c. the nadph oxidase reaction measured ...19968995793
[description of the colonization of aedes albopictus (diptera: culicidae) in the region of saõ josé do rio preto, sp, 1991-1994].this study aims to describe and analyze the colonization of aedes albopictus whose presence was detected in 1991 in the area of sào josé do rio. preto already colonized by the aedes aegypti. year and month of occurrence, counties, composition and location of larval samples, kinds of containers, average number of larvae an breteau index bare been analyzed from the information obtained in measurements of larval density by superintendência de controle de endemias (sucen). the presence of aedes albo ...19969011878
mosquito hexamerins: characterization during larval development.we report here the first examination of hexamerins expressed during mosquito larval development. haemolymph proteins from fourth-instar larvae of six species representing the two major subfamilies of mosquitoes were characterized by immunoblotting using antisera to calliphorin, the major hexamerin of the blowfly. calliphora vicina, or to lsp1 or lsp2, the two distinct hexamerins of drosophila melanogaster. in each mosquito species the antisera demonstrated the presence of multiple abundant hexam ...19979013251
juvenile hormone controls early trypsin gene transcription in the midgut of aedes aegypti.early trypsin is a female-specific protease present in the aedes aegypti midgut during the first few hours after ingestion of a blood meal. the enzymatic activity of early trypsin plays an essential role in the transcriptional activation of the late trypsin gene, which encodes the major midgut endoprotease involved in blood meal protein digestion. transcription of the early trypsin gene is part of the normal post-emergence maturation of the midgut in the adult female. abdominal ligation within 1 ...19979013256
bioactive compounds from taiwania cryptomerioides.two new sesquiterpenes, 10 alpha-hydroxyamorphan-4-en-3-one (1) and 4 alpha-methylcadinane-4 alpha-methyl-1 alpha,2 alpha,10 alpha-triol (2), together with four known compounds, sesquiterpenes 10 alpha-hydroxycadinan-4-en-3-one (3) and alpha-cadinol (4), diterpene ferruginol and lignan helioxanthin, were isolated from the whole plant of taiwania cryptomerioides under bioassay-guided fractionations. the structures of 1 and 2 were elucidated mainly by the nmr spectroscopic analyses. bioactivities ...19979014350
transovarial transmission of dengue 3 virus by aedes aegypti. 19969015501
identification of a gene for cyt1a-like hemolysin from bacillus thuringiensis subsp. medellin and expression in a crystal-negative b. thuringiensis strain.a gene designated cyt1ab1, encoding a 27,490-da protein, was isolated from bacillus thuringiensis subsp. medellin (h30 serotype) by using an oligonucleotide probe corresponding to the cyt1aa1 gene. the sequence of the cyt1ab1 protein, as deduced from the sequence of the cyt1ab1 gene, was 86% identical to that of the cyt1aa1 protein and 32% identical to that of the cyt2aa1 protein from b. thuringiensis subsp. kyushuensis. the cyt1ab1 gene was flanked upstream by a p21 gene, in the same orientatio ...19979023925
[detection of aedes aegypti and aedes albopictus, in an urban zone of the municipality of catanduva, sp, after control of a dengue epidemic].after the realization of control research that had in view the transmission of dengue virus, we started to monitor two kinds of entomological vigilance, breteau index and ovitrap. we intended to evaluate the necessary time elapsed before aedes sp mosquitoes were again detected at the urban area of catanduva s town (sp). the ovitraps showed positiveness for the aedes aegypti two months after the control research, while the breteau index became positive only at the fourth month after the end of th ...19969026829
characterization of aedes communis, aedes aegypti and anopheles stephensi mosquito saliva antigens by immunoblotting.mosquito bites cause immediate wheals and delayed bite papules in sensitized subjects having saliva-specific ige and igg4 antibodies. at present, mosquito saliva antigens are not well characterized.19979030098
toxicity of insecticides to toxorhynchites splendens and three vector mosquitos and their sublethal effect on biocontrol potential of the predator.toxicity of six larvicides ie fenthion, temephos, malathion, deltamethrin, alphamethrin (fendona), oms 3031 and five adulticides ie malathion, fenitrothion, propoxur, deltamethrin, permethrin to aedes aegypti, culex quiquefasciatus, anopheles stephensi and the predator, toxorhynchites splendens was studied for evaluating safety margin. concentrations of alphamethrin that killed 50% larvae of t. splendens were 53 and 12 times more than that which killed cx. quinquefasciatus and ae. aegypti. in ca ...19969031419
dengue vector mosquitos at a tourist attraction, ko samui, in 1995.on ko samui, thailand there were two epidemics of dengue hemorrhagic fever (dhf) in 1966 and 1967, followed by endemics up to 1994. aedes aegypti and aedes albopictus were the vectors. from january to july 1995, 51 cases of dhf were reported, out of these were many foreigners who still suffer from dengue fever and return home with negative impression. we carried out an entomological survey around the island and collected the mosquitos to detect dengue virus by digoxigenin-cdna probe. the data re ...19969031420
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