Publications

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first record of aedes aegypti and aedes albopictus in thirteen panhandle region counties of texas, u.s.a. 201729125249
emergent acoustic order in arrays of mosquitoes.the yellow fever mosquito aedes aegypti forms aerial swarms that serve as mating aggregations [1]. despite lacking the remarkable collective order of other animal ensembles, such as fish and birds [2], the kinematic properties of these swarms bear the hallmarks of local interaction and global cohesion [3,4]. however, the mechanisms responsible for collective behaviour in mosquitoes are not well understood. mosquitoes use their antennae as hearing organs to locate and interact with one another vi ...201729161555
dengue virus detection in aedes aegypti larvae and pupae collected in rural areas of anapoima, cundinamarca, colombia.there is a high incidence and prevalence of dengue in the department of cundinamarca, and recently aedes aegypti, the main vector of dengue virus (denv), was detected in some of its rural areas.201729161491
effect of the type and number of washes in the efficacy of long lasting insecticidal nets for aedes aegypti control in girardot, colombia.the use of long lasting insecticidal materials has shown promising results in the control of aedes aegypti.201729161489
virological surveillance of aedes (stegomyia) aegypti and aedes (stegomyia) albopictus as support for decision making for dengue control in medellín.dengue represents an important public health problem in colombia. no treatment is available and the vaccine has not been approved in all countries, hence, actions should be strengthened to mitigate its impact through the control of aedes aegypti, the vector mosquito. in colombia, surveillance is done using entomological indexes and case notification, which is usually informed late, leading to untimely interventions. viral detection in urban mosquitoes using molecular techniques provides more acc ...201729161487
aedes albopictus (skuse, 1894) infected with the american-asian genotype of dengue type 2 virus in medellín suggests its possible role as vector of dengue fever in colombia.aedes aegypti and ae. albopictus are recognized vectors of dengue, yellow fever, chikungunya and zika arboviruses in several countries worldwide. in colombia, ae. albopictus geographical distribution has increased to include highly populated cities such as cali and medellín. although this species has been frequently found in urban and semi-urban zones in the country, its role as vector of the dengue fever is poorly known.201729161485
[laboratory susceptibility tests of aedes aegypti and culex quinquefasciatus larvae to the entomopathogenic nematode heterorhabditis bacteriophora].aedes aegypti is the vector of dengue, yellow fever, zika and chikungunya viruses, and culex quinquefasciatus is the vector of st. louis and west nile encephalitis viruses.201729161479
[spatial distribution of potential and positive aedes aegypti breeding sites].the spatial distribution of aedes aegypti is heterogeneous, and the interaction between positive and potential breeding sites located both inside and outside homes is one of the most difficult aspects to characterize in vector control programs.201729161478
[spatial distribution of aedes aegypti (diptera: culicidae) in the rural area of two municipalities of cundinamarca, colombia].it is difficult to adapt or create new strategies for dengue control in rural areas due to the dispersion of homes and the lack of knowledge about the presence and behavior of the vector in these areas. spatial data analysis could help understand time and space dynamics related to the transmission of vector-borne diseases in rural areas and, thus, contribute to the prevention and control of dengue.201729161476
alternative control of aedes aegypti resistant to pyrethroids: lethal and sublethal effects of monoterpenes bioinsecticides.the mosquito aedes aegypti is intensely controlled because it is vector of viruses that cause innumerous diseases, especially in tropical regions. due to the indiscriminate use of insecticides, populations from different regions have been resistant to pyrethroids. here, we analyzed the lethal and sublethal effects of essential oil of aristolochia trilobata and its major compounds on a. aegypti from susceptible and pyrethroid resistant populations.201729160036
upregulation of aedes aegypti vago1 by wolbachia and its effect on dengue virus replication.dengue infection along with its related disease conditions poses a significant threat to human health. the pathogen responsible for this infection is dengue virus (denv) which is primarily transmitted to humans through the bites of aedes aegypti mosquitoes. unavailability of a potent vaccine has recently sparked renewed research endeavours aimed at vector control. to date, wolbachia as an endosymbiotic bacterium has shown promise as a novel biocontrol agent to restrict denv replication in the ve ...201729157676
a call to introduce structured zika surveillance in india.india has the climatic conditions conducive to year-round transmission of zika virus, and a structured disease surveillance program should be implemented to prevent an outbreak. such a program should (i) start screening before an outbreak arises; (ii) collect baseline data to assess future disease risk and monitor potential birth defects; and (iii) provide new insights into the ecology of the disease and inform public health policy following the one health concept.201729153262
effective population sizes of a major vector of human diseases, aedes aegypti.the effective population size (ne ) is a fundamental parameter in population genetics that determines the relative strength of selection and random genetic drift, the effect of migration, levels of inbreeding, and linkage disequilibrium. in many cases where it has been estimated in animals, ne is on the order of 10%-20% of the census size. in this study, we use 12 microsatellite markers and 14,888 single nucleotide polymorphisms (snps) to empirically estimate ne in aedes aegypti, the major vecto ...201729151858
vectors, hosts, and control measures for zika virus in the americas.we examine zika virus (zikv) from an ecological perspective and with a focus on the americas. we assess (1) the role of wildlife in zikv disease ecology, (2) how mosquito behavior and biology influence disease dynamics, and (3) how nontarget species and ecosystems may be impacted by vector control programs. our review suggests that free-ranging, non-human primates may be involved in zikv transmission in the old world; however, other wildlife species likely play a limited role in maintaining or t ...201729150828
the introduction of dengue follows transportation infrastructure changes in the state of acre, brazil: a network-based analysis.human mobility, presence and passive transportation of aedes aegypti mosquito, and environmental characteristics are a group of factors which contribute to the success of dengue spread and establishment. to understand this process, we assess data from dengue national and municipal basins regarding population and demographics, transportation network, human mobility, and ae. aegypti monitoring for the brazilian state of acre since the first recorded dengue case in the year 2000 to the year 2015. d ...201729149175
baccharis reticularia dc. and limonene nanoemulsions: promising larvicidal agents for aedes aegypti (diptera: culicidae) control.baccharis reticularia dc. is a plant species from the asteraceae family that is endemic to brazil. despite the great importance of baccharis genus, no study has been carried out regarding either the phytochemical composition of b. reticularia or the evaluation of its larvicidal potential. considering the intrinsic immiscibility of essential oils, this study shows larvicidal nanoemulsions containing the b. reticularia phytochemically characterized essential oil and its main constituent against ae ...201729149027
[dengue fever : symptoms, epidemiology, entomology, pathogen diagnosis and prevention].dengue infections are among the most frequent causes of febrile disease in tropical climates. infections are caused by a flavivirus transmitted by the aedes mosquito. aedes aegypti mosquitos are the main transmitters of dengue viruses. since these insects are both diurnal and container breeders, particular prevention and control measures are required. symptom severity varies and can range from a mild, flu-like clinical picture to severe hemorrhage and shock. the most common symptoms experienced ...201729147722
development and physiological effects of an artificial diet for wolbachia-infected aedes aegypti.the endosymbiotic bacterium wolbachia spreads rapidly through populations of aedes aegypti mosquitoes, and strongly inhibits infection with key human pathogens including the dengue and zika viruses. mosquito control programs aimed at limiting transmission of these viruses are ongoing in multiple countries, yet there is a dearth of mass rearing infrastructure specific to wolbachia-infected mosquitoes. one example is the lack of a blood meal substitute, which accounts for the wolbachia-specific ph ...201729146940
behavioral, climatic, and environmental risk factors for zika and chikungunya virus infections in rio de janeiro, brazil, 2015-16.the burden of arboviruses in the americas is high and may result in long-term sequelae with infants disabled by zika virus infection (zikv) and arthritis caused by infection with chikungunya virus (chikv). we aimed to identify environmental drivers of arbovirus epidemics to predict where the next epidemics will occur and prioritize municipalities for vector control and eventual vaccination. we screened sera and urine samples (n = 10,459) from residents of 48 municipalities in the state of rio de ...201729145452
zika virus: an updated review of competent or naturally infected mosquitoes.zika virus (zikv) is an arthropod-borne virus (arbovirus) that recently caused outbreaks in the americas. over the past 60 years, this virus has been observed circulating among african, asian, and pacific island populations, but little attention has been paid by the scientific community until the discovery that large-scale urban zikv outbreaks were associated with neurological complications such as microcephaly and several other neurological malformations in fetuses and newborns. this paper is a ...201729145400
shifting the paradigm in dirofilaria immitis prevention: blocking transmission from mosquitoes to dogs using repellents/insecticides and macrocyclic lactone prevention as part of a multimodal approach.this study assessed the influence of a topical ectoparasiticide (dinotefuran-permethrin-pyriproxyfen, dpp, vectra® 3d, ceva animal health) combined with a macrocyclic lactone (milbemycin oxime, mbo, interceptor®, virbac) on transmission of heartworm l3 from mosquitoes to dogs and subsequent development of worms in treated dogs exposed to infected mosquitoes.201729143678
blocking the transmission of heartworm (dirofilaria immitis) to mosquitoes (aedes aegypti) by weekly exposure for one month to microfilaremic dogs treated once topically with dinotefuran-permethrin-pyriproxyfen.this study assessed the influence of a topical ectoparasiticide (dinotefuran-permethrin-pyriproxyfen, dpp, vectra®3d, ceva animal health) on the acquisition of heartworm microfilariae by mosquitoes exposed to microfilaremic dogs weekly for 1 month.201729143651
corrigendum: rna-seq comparison of larval and adult malpighian tubules of the yellow fever mosquito aedes aegypti reveals life stage-specific changes in renal function.[this corrects the article on p. 283 in vol. 8, pmid: 28536536.].201729142533
the importance of human population characteristics in modeling aedes aegypti distributions and assessing risk of mosquito-borne infectious diseases.the mosquito aedes aegypti has long been a vector for human illness in the southeastern united states. in the past, it has been responsible for outbreaks of dengue, chikungunya, and yellow fever and, very recently, the zika virus that has been introduced to the region. multiple studies have modeled the geographic distribution of ae. aegypti as a function of climate factors; however, this ignores the importance of humans to the anthropophilic biter. furthermore, ae. aegypti thrives in areas where ...201729167627
determination of the efficiency of diets for larval development in mass rearing aedes aegypti (diptera: culicidae).larval diet quality and rearing conditions have a direct and irreversible effect on adult traits. therefore, the current study was carried out to optimize the larval diet for mass rearing of aedes aegypti, for sterile insect technique (sit)-based applications in sri lanka. five batches of 750 first instar larvae (l 1) of ae. aegypti were exposed to five different concentrations (2-10%) of international atomic energy agency (iaea) recommended the larval diet. morphological development parameters ...201729166980
biology of two larval morphological phenotypes of aedes aegypti in abidjan, côte d'ivoire.since 2008, several outbreaks of yellow fever and dengue occurred in abidjan, the economic capital of côte d'ivoire. a better knowledge of the biology of aedes aegypti populations, the main vector of yellow fever and dengue viruses, is necessary to tailor vector control strategies implemented in the city. this study was designed to determine some biological parameters, occurring during the life cycle of two morphological phenotypes of ae. aegypti larvae. mosquitoes were sampled in a suburb of ab ...201729166962
feeding-induced changes in allatostatin-a and short neuropeptide f in the antennal lobes affect odor-mediated host seeking in the yellow fever mosquito, aedes aegypti.aedes aegypti is a model species in which the endogenous regulation of odor-mediated host seeking behavior has received some attention. sugar feeding and host seeking in female a. aegypti are transiently inhibited following a blood meal. this inhibition is partially mediated by short neuropeptide f (snpf). the paired antennal lobes (als), as the first processing centers for olfactory information, has been shown to play a significant role in the neuropeptidergic regulation of odor-mediated behavi ...201729166650
wolbachia infection alters the relative abundance of resident bacteria in adult aedes aegypti mosquitoes, but not larvae.insect-symbiont interactions are known to play key roles in host functions and fitness. the common insect endosymbiont wolbachia can reduce the ability of several human pathogens, including arboviruses and the malaria parasite, to replicate in insect hosts. wolbachia does not naturally infect aedes aegypti, the primary vector of dengue virus, but transinfected ae. aegypti have antidengue virus properties and are currently being trialled as a dengue biocontrol strategy. here, we assess the impact ...201729165845
insecticide susceptibility status in mexican populations of stegomyia aegypti (= aedes aegypti): a nationwide assessment.in mexico, mosquito vector-borne diseases are of public health concern as a result of their impact on human morbidity and mortality. the use of insecticides against adult mosquitoes is one of the most common ways of controlling mosquito population densities. however, the use of these compounds has resulted in the development of insecticide resistance. the aim of this study was to estimate susceptibility to six pyrethroids, two carbamates and two organophosphates in mexican populations of stegomy ...201729165810
larvicidal, histopathological efficacy of penicillium daleae against larvae of culex quinquefasciatus and aedes aegypti plus biotoxicity on artemia nauplii a non-target aquatic organism.mosquitoes can transmit the terrible diseases to human beings. soil-borne fungal products act as potential source for low-cost chemicals, used for developing eco-friendly control agents against mosquito-vector borne diseases. the prime aim of study was to check the larvicidal potential of fungus mycelia (by ethyl acetate solvent) extract from penicillium daleae (kx387370) against culex quinquefasciatus and aedes aegypti and to test the toxicity of brine shrimp artemia nauplii, by observing the p ...201729163159
involvement of cecropin b in the formation of the aedes aegypti mosquito cuticle.in this study, we found a mosquito antimicrobial peptide (amp), aedes aegypti cecropin b (aacec b), was expressed constitutively in pupae. knockdown in the pupae of aacec b using double-stranded rna (dsrna) resulted in high mortality, the emergence of deformed adults and an impairment of pharate adult cuticle formation with fewer lamellae being deposited and the helicoidal pattern of the chitin microfibrils being disorganized. simultaneous injection of aacec b dsrna and aacec b peptide into pupa ...201729180688
a peptide signaling system that rapidly enforces paternity in the aedes aegypti mosquito.female aedes aegypti mosquitoes typically mate only once with one male in their lifetime, a behavior known as "monandry" [1]. this single mating event provisions the female with sufficient sperm to fertilize the >500 eggs she will produce during her ∼4- to 6-week lifespan in the laboratory [2]. successful mating induces lifetime refractoriness to subsequent insemination by other males, enforcing the paternity of the first male [3-5]. ae. aegypti mate in flight near human hosts [6], and females b ...201729174895
insecticide susceptibility screening against culex and aedes (diptera: culicidae) mosquitoes from the united states.mosquitoes exposed to sublethal doses of insecticides may be selected for resistance to insecticide active ingredients (ais). mosquitoes are exposed to ais through agricultural, public/private mosquito control programs, homeowners, and other sources. hence, mosquito control programs should routinely measure the resistance/susceptibility status of mosquito populations of public health concern. the objectives here were to determine resistance status for six ais used in adult mosquito control in th ...201729186579
aedes aegypti microrna mir-2b regulates ubiquitin-related modifier to control chikungunya virus replication.arboviruses that replicate in mosquitoes activate innate immune response within mosquitoes. regulatory non-coding micrornas (mirna) are known to be modulated in mosquitoes during chikungunya infection. however, information about targets of these mirnas is scant. the present study was aimed to identify and analyze targets of mirnas that are regulated during chikungunya virus (chikv) replication in aedes aegypti cells and in the mosquito. employing next-generation sequencing technologies, we ident ...201729247247
zika virus can be venereally transmitted between aedes aegypti mosquitoes.alternative transmission routes have been described for zika virus (zikv). here, we assessed for the first time the venereal transmission of zikv between aedes aegypti under laboratory conditions.201729246237
full-genome dengue virus sequencing in mosquito saliva shows lack of convergent positive selection during transmission byaedes aegypti.like other pathogens with high mutation and replication rates, within-host dengue virus (denv) populations evolve during infection of their main mosquito vector,aedes aegypti. within-host denv evolution during transmission provides opportunities for adaptation and emergence of novel virus variants. recent studies of denv genetic diversity failed to detect convergent evolution of adaptive mutations in mosquito tissues such as midgut and salivary glands, suggesting that convergent positive selecti ...201729497564
perturbed cholesterol and vesicular trafficking associated with dengue blocking in wolbachia-infected aedes aegypti cells.wolbachia are intracellular maternally inherited bacteria that can spread through insect populations and block virus transmission by mosquitoes, providing an important approach to dengue control. to better understand the mechanisms of virus inhibition, we here perform proteomic quantification of the effects of wolbachia in aedes aegypti mosquito cells and midgut. perturbations are observed in vesicular trafficking, lipid metabolism and in the endoplasmic reticulum that could impact viral entry a ...201728904344
minimum infectious dose for chikungunya virus in aedes aegypti and ae. albopictus mosquitoes.understanding the ability of the chikungunya virus (chikv) to be transmitted by aedes vectors in the americas is critical for assessing epidemiological risk. one element that must be considered is the minimum infectious dose of virus that can lead to transmission following the extrinsic incubation period. this study aimed to determine the minimum infection rate for the two aedes species studied. the results revealed that doses as low as 3.9 log10 plaque-forming units per ml (pfu/ml) of an asian ...201728902278
first detection of dengue and chikungunya viruses in natural populations of aedes aegypti in martinique during the 2013 - 2015 concomitant outbreak.dengue and chikungunya viruses are transmitted by aedes mosquitoes. in martinique, an island of the french west indies, aedes aegypti is the suspected vector of both arboviruses; there is no aedes albopictus on the island. during the concomitant outbreak of 2013 - 2015, the authors collected wild a. aegypti populations, and for the first time, detected dengue and chikungunya viruses in field-collected females. this paper demonstrates the mosquito's role in transmission of both dengue and chikung ...201728902276
highly effective broad spectrum chimeric larvicide that targets vector mosquitoes using a lipophilic protein.two mosquitocidal bacteria, bacillus thuringiensis subsp. israelensis (bti) and lysinibacillus sphaericus (ls) are the active ingredients of commercial larvicides used widely to control vector mosquitoes. bti's efficacy is due to synergistic interactions among four proteins, cry4aa, cry4ba, cry11aa, and cyt1aa, whereas ls's activity is caused by bin, a heterodimer consisting of bina, the toxin, and binb, a midgut-binding protein. cyt1aa is lipophilic and synergizes bti cry proteins by increasing ...201728900215
efficacy of blood sources and artificial blood feeding methods in rearing of aedes aegypti (diptera: culicidae) for sterile insect technique and incompatible insect technique approaches in sri lanka.selection of the artificial membrane feeding technique and blood meal source has been recognized as key considerations in mass rearing of vectors.201728894749
a natural hybrid of a bacillus thuringiensis cry2a toxin implicates domain i in specificity determination.a pcr-rflp method was used to identify cry2a toxin genes in a collection of 300 strains of bacillus thuringiensis. from 81 genes identified, the vast majority appeared to be cry2aa or cry2ab, however three showed a different pattern and were subsequently cloned and sequenced. the gene cloned from strain hd395 was named cry2ba2. since the proteins encoded by the genes cloned from ls5115-3 and ds415 shared >95% sequence identity with existing toxins their genes were named cry2aa17 and cry2ab29 res ...201728888766
isolation of a larvicidal compound from piper solmsianum c.dc. (piperaceae).the aedes aegypti mosquito is one of the major vectors of arboviruses. these diseases have re-emerged and the insecticides used nowadays are toxic to mammals and environment and have only been effective in the short-term. in this context, natural products are an alternative. the genus piper has many active compounds against arthropods, including neolignans. the present study evaluated the larvicidal potential of the n-hexanic extract of piper solmsianum and eupomatenoid-6, identified by gc-ms an ...201728882070
association between suspected zika virus disease during pregnancy and giving birth to a newborn with congenital microcephaly: a matched case-control study.in early 2015, an outbreak of an acute exanthematous illness with dengue-like symptoms occurred in northeastern brazil. by the end of the same year, an unexpected increase in the number of cases of microcephaly was observed in the region. the microcephaly outbreak cause was unknown and rumors pointing to various potential causes arose. since we were unaware at the time if this scenario would attract the interest of the broader scientific community, due to the neglected regions associated and as ...201728877754
microrna-277 targets insulin-like peptides 7 and 8 to control lipid metabolism and reproduction in aedes aegypti mosquitoes.hematophagous female mosquitoes transmit numerous devastating human diseases, including malaria, dengue fever, zika virus, and others. because of their obligatory requirement of a vertebrate blood meal for reproduction, these mosquitoes need a lot of energy; therefore, understanding the molecular mechanisms linking metabolism and reproduction is of particular importance. lipids are the major energy store providing the fuel required for host seeking and reproduction. they are essential components ...201728874536
updated reported distribution of aedes (stegomyia) aegypti and aedes (stegomyia) albopictus (diptera: culicidae) in the united states, 1995-2016.aedes (stegomyia) aegypti (l.) and aedes (stegomyia) albopictus (skuse) are potential vectors of zika, dengue, and chikungunya viruses in the united states. a zika virus outbreak in florida in the summer of 2016, driven by ae. aegypti and resulting in > 200 locally acquired cases of human illness, underscored the need for up-to-date information on the geographic distribution of ae. aegypti and ae. albopictus in the united states. in early 2016, we conducted a survey and literature review to comp ...201728874014
"we need people to collaborate together against this disease": a qualitative exploration of perceptions of dengue fever control in caregivers' of children under 5 years, in the peruvian amazon.dengue fever presents a significant and growing burden of disease to endemic countries, where children are at particular risk. worldwide, no effective anti-viral treatment has been identified, thus vector control is key for disease prevention, particularly in peru where no vaccine is currently available. this qualitative study aimed to explore the perceptions of dengue control in caregivers' of children under 5 years in peru, to help direct future mosquito control programmes and strategy.201728873408
fat body organ culture system in aedes aegypti, a vector of zika virus.the insect fat body plays a central role in insect metabolism and nutrient storage, mirroring functions of the liver and fat tissue in vertebrates. insect fat body tissue is usually distributed throughout the insect body. however, it is often concentrated in the abdomen and attached to the abdominal body wall. the mosquito fat body is the sole source of yolk proteins, which are critical for egg production. therefore, the in vitro culture of mosquito fat body tissues represents an important syste ...201728872112
evaluation of ecdysteroid antisera for a competitive enzyme immunoassay and extraction procedures for the measurement of mosquito ecdysteroids.ecdysteroid hormones regulate several aspects of insect development and reproduction. the predominant ecdysteroids produced by insects including mosquitoes are ecdysone (e) and 20-hydroxyecdysone (20e). the ability to measure e and 20e titers is essential for many studies, but few sensitive, low cost options are currently available for doing so. to address this deficiency, we developed a new enzyme-linked immunoassay (eia). in the first part of the study, we compared the affinity of two new anti ...201728866256
aedes aegypti saliva enhances chikungunya virus replication in human skin fibroblasts via inhibition of the type i interferon signaling pathway.chikungunya virus (chikv) transmission occurs through the bite of an infected aedes mosquito which injects virus-containing saliva into the skin of the human host during blood feeding. in the present study, we have determined the effect of aedes aegypti saliva on chikv replication in human skin fibroblasts, a major cell type for viral entry, which mimics the events that occur during natural transmission. a significant increase in the expression of viral transcripts and infectious viral particles ...201728866137
presence of a predator image in potential breeding sites and oviposition responses of a dengue vector.in dengue vector control, attempts to minimize or replace the use of pesticides have mostly involved use of predators, but success has been severely impeded by difficulties associated with financial and environmental costs, predator mass production, and persistence in target habitats. visual deterrents have been used successfully to control animal pests, in some cases in an effort to replace pesticide use. despite evidence that visual signals are crucial in site choice for egg deposition by deng ...201728865898
correction: insecticide resistance to permethrin and malathion and associated mechanisms in aedes aegypti mosquitoes from st. andrew jamaica.[this corrects the article doi: 10.1371/journal.pone.0179673.].201728859159
effect of nozzle orientation on dispersion of aqualuer 20-20 sprayed by a truck-mounted ultra-low volume sprayer against caged aedes aegypti.inconsistencies in efficacy of ultra-low volume (ulv) ground applications in the literature are linked to the lack of adjustments in sprayer parameters. to investigate the effect of nozzle orientation of a truck-mounted ulv sprayer on application efficacy, a study was conducted at st. johns county fairground, elkton, fl, during the summer of 2014. the efficacy was assessed by mortality of caged adult aedes aegypti, spray deposition, and droplet size spectrum up to 122 m from the spray line. aqua ...201728854107
larvicidal and ovicidal activity of terminalia chebula retz. (family: combretaceae) medicinal plant extracts against anopheles stephensi, aedes aegypti and culex quinquefasciatus.insect-borne diseases remain to this day a major source of illness and death worldwide. the resistance to chemical insecticides among mosquito species has been considered as a setback in vector control. mosquito control programs, botanical origin may have the potential to eliminate eggs and larvae. so, the larvicidal and ovicidal activities of crude benzene, hexane, ethyl acetate, chloroform and methanol extracts of terminalia chebula were assayed for their toxicity against three important vecto ...201728848262
improving the built environment in urban areas to control aedes aegypti-borne diseases. 201728804174
first report of coelomomyces santabrancae sp. nov. (blastocladiomycetes: blastocladiales) infecting mosquito larvae (diptera: culicidae) in central brazil.a project from 2013 to 2017 sought to discover pathogenic fungi and oomycetes from dipteran species that are vectors of major diseases of humans and animals in central brazil and to begin evaluating the potential of these pathogens as potential biological control agents concentrated on mosquito larvae. some collecting sites proved to be especially productive for pathogens of naturally occurring mosquito species and for placements of healthy sentinel larvae of aedes aegypti in various sorts of co ...201728803981
dinuclear silver(i)-n-heterocyclic carbene complexes: synthesis, characterization and larvicidal activity of bis-imidazolium dinuclear silver(i)-n-heterocyclic carbene complexes.new synthesized bis-imidazolium salts that are linked by xylyl derivatives moiety, 1-4 was reacted with ag2o to facilitate the formation of dinuclear ag(i)-n-heterocyclic carbene (nhc) complexes, 5-8, respectively. all the synthesized ligand salts and complexes were characterized by 1h and 13c nmr, ftir spectroscopy and elemental analysis. molecular structures of compounds 3, 5, and 7 were elucidated by single crystal x-ray diffraction analyses. larvicidal studies against the aedes aegypti and c ...201728800547
preparation of a nanoemulsion with carapa guianensis aublet (meliaceae) oil by a low-energy/solvent-free method and evaluation of its preliminary residual larvicidal activity.andiroba (carapa guianensis) seeds are the source of an oil with a wide range of biological activities and ethnopharmacological uses. however, few studies have devoted attention to innovative formulations, including nanoemulsions. the present study aimed to obtain a colloidal system with the andiroba oil using a low-energy and organic-solvent-free method. moreover, the preliminary residual larvicidal activity of the nanoemulsion against aedes aegypti was evaluated. oleic and palmitic acids were ...201728798803
mosquito species occurrence in association with landscape composition in green urban areas.aedes aegypti prefers densely populated habitats, but has been shown to explore less anthropogenic environments. we investigated composition of the abundance of mosquitoes in forested areas and assessed relationships between species occurrences and different types of land use and land cover at three spatial scales (100m, 500m and 1000m). mosquitoes were collected from october 2012 to march 2013 using oviposition traps. we collected 4,179 mosquitoes in total including at least 10 species. aedes a ...201728793030
insecticide resistance, associated mechanisms and fitness aspects in two brazilian stegomyia aegypti (= aedes aegypti) populations.in brazil, insecticide resistance in stegomyia aegypti (= aedes aegypti) (diptera: culicidae) populations to pyrethroids and to the organophosphate (op) temephos is disseminated. currently, insect growth regulators (igrs) and the op malathion are employed against larvae and adults, respectively. bioassays with mosquitoes from two northeast municipalities, crato and aracaju, revealed, in both populations, susceptibility to igrs and malathion (rr95  ≤ 2.0), confirming the effectiveness of these co ...201728752548
a mosquito hemolymph odorant-binding protein family member specifically binds juvenile hormone.juvenile hormone (jh) is a key regulator of insect development and reproduction. in adult mosquitoes, it is essential for maturation of the ovary and normal male reproductive behavior, but how jh distribution and activity is regulated after secretion is unclear. here, we report a new type of specific jh-binding protein, given the name mosquito juvenile hormone-binding protein (mjhbp), which circulates in the hemolymph of pupal and adult aedes aegypti males and females. mjhbp is a member of the o ...201728751377
rhanterium epapposum oliv. essential oil: chemical composition and antimicrobial, insect-repellent and anticholinesterase activities.the essential oil (eo) of the aerial parts of rhanterium epapposum oliv. (asteraceae), was obtained by hydrodistillation. the oil was subsequently analyzed by both gc-fid and gc-ms, simultaneously. forty-five components representing 99.2% of the oil composition were identified. the most abundant compounds were camphene (38.5%), myrcene (17.5%), limonene (10.1%) and α-pinene (8.7%). referring to the ethnobotanical utilization, an insecticidal assay was performed, where the oil repelled the yellow ...201728725142
effects of rearing salinity on expression and function of ion-motive atpases and ion transport across the gastric caecum of aedes aegypti larvae.larvae of aedes aegypti, the yellow fever vector, inhabit a variety of aquatic habitats ranging from freshwater to brackish water. this study focuses on the gastric caecum of the larvae, an organ that has not been widely studied. we provide the first measurements of h+, k+ and na+ fluxes at the distal and proximal gastric caecum, and have shown that they differ in the two regions, consistent with previously reported regionalization of ion transporters. moreover, we have shown that the regionaliz ...201728659305
insecticide resistance to permethrin and malathion and associated mechanisms in aedes aegypti mosquitoes from st. andrew jamaica.the emergence of novel diseases spread by the aedes aegypti mosquito in jamaica and the caribbean, has prompted studies on insecticide resistance towards effective management of the vector. though jamaica has been using the organophosphate insecticide malathion in its vector control program for more than 30 years, resistance to the pesticide has not been tested in over a decade. we analyzed resistance to malathion and the pyrethroid insecticide, permethrin on mosquitoes collected across st. andr ...201728650966
data from salivary gland proteome analysis of female aedes aegypti linn.salivary gland proteins from female aedes aegypti mosquito were extracted and analyzed on high-resolution mass spectrometry. proteomic data was analysed using two search algorithms sequest and mascot, which results in acquisition of 83,836 spectra which were assigned to 5417 peptides belonging to 1208 proteins.these proteins were then assigned molecular functions and further analysis revealed biological processes they are involved in using gene ontology annotations. several immunity related path ...201728649586
[zika: probability of establishment of its vector, aedes aegypti, in chile].the zika virus has raised world alarm in recent years, representing a major public health problem. in this study we evaluated the potential risk of exposure to zika virus in chile, associated with the probability of establishment of the vector aedes aegypti in the country. niche modelling techniques were used to project the bioclimatic requirements of the vector (global niches), identifying zones of high suitability for the species within the country. then, the potential distribution of the vect ...201729488548
laboratory evaluation of a novel lethal ovitrap for control of aedes aegypti.aedes aegypti (l.) (diptera: culicidae) are known to flourish in a variety of natural and residential habitats and are competent vectors of at least 22 different arboviruses, including dengue, chikungunya, and zika. their global distribution, anthropophilic nature, and vector competency make them species of interest for control. a novel durable dual-action lethal ovitrap (ddalo) with combined larviciding and adulticiding effects, as well as a slow-release polymer (isobutyl methacrylate), was des ...201728968892
insecticidal toxicities of carvacrol and thymol derived from thymus vulgaris lin. against pochazia shantungensis chou &lu., newly recorded pest.the insecticidal toxicities of five essential oils against pochazia shantungensis adults and nymphs, newly recorded pests, were evaluated. the lc50 values of thymus vulgaris, ruta graveolens, citrus aurantium, leptospermum petersonii and achillea millefolium oils were recorded as 57.48, 84.44, 92.58, 113.26 and 125.78 mg/l, respectively, against p. shantungensis nymphs using the leaf dipping bioassay, and 75.80, 109.86, 113.26, 145.06 and 153.74 mg/l, respectively, against p. shantungensis adult ...201728106093
rna interference mediated knockdown of brugia malayi udp-galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae.galactofuranose is an essential cell surface component present in bacteria, fungi and several nematodes such as caenorhabditis spp., brugia spp., onchocerca spp. and strongyloides spp. this sugar maintains the integrity of parasite surface and is essential for virulence. udp-galactopyranose mutase (bmugm) plays a key role in galf biosynthesis by catalyzing conversion of udp-galactopyranose into udp-galactofuranose and knockout studies of the gene in leishmania major, mycobacterium and aspergillu ...201728103957
antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections.the transmission of dengue virus (denv) and chikungunya virus (chikv) has increased worldwide, due in part to the lack of a specific antiviral treatment. for this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research priority. the objective of this study was to identify some of the compounds that are present in mammea americana (m. americana) and tabernaemontana cymosa (t. cymosa) plants and, subsequently, to evaluate their cyto ...201728100218
microrna-34 directly targets pair-rule genes and cytoskeleton component in the honey bee.micrornas (mirnas) are key regulators of developmental processes, such as cell fate determination and differentiation. previous studies showed dicer knockdown in honeybee embryos disrupt the processing of functional mature mirnas and impairs embryo patterning. here we investigated the expression profiles of mirnas in honeybee embryogenesis and the role of the highly conserved mir-34-5p in the regulation of genes involved in insect segmentation. a total of 221 mirnas were expressed in honey bee e ...201728098233
assessment of synthetic floral-based attractants and sugar baits to capture male and female aedes aegypti (diptera: culicidae).the viruses transmitted by aedes aegypti, including dengue and zika viruses, are rapidly expanding in geographic range and as a threat to public health. in response, control programs are increasingly turning to the use of sterile insect techniques resulting in a need to trap male ae. aegypti to monitor the efficacy of the intervention. however, there is a lack of effective and cheap methods for trapping males. thus, we attempted to exploit the physiological need to obtain energy from sugar feedi ...201728095875
identification of functional enolase genes of the silkworm bombyx mori from public databases with a combination of dry and wet bench processes.various insect species have been added to genomic databases over the years. thus, researchers can easily obtain online genomic information on invertebrates and insects. however, many incorrectly annotated genes are included in these databases, which can prevent the correct interpretation of subsequent functional analyses. to address this problem, we used a combination of dry and wet bench processes to select functional genes from public databases.201728086791
application of the analytic hierarchy approach to the risk assessment of zika virus disease transmission in guangdong province, china.an international spread of zika virus (zikv) infection has attracted global attention in 2015. the infection also affected guangdong province, which is located in southern china. multiple factors, including frequent communication with south america and southeast asia, suitable climate (sub-tropical) for the habitat of aedes species, may increase the risk of zikv disease transmission in this region.201728086897
functional characterization of the active mutator-like transposable element, muta1 from the mosquito aedes aegypti.mutator-like transposable elements (mules) are widespread with members in fungi, plants, and animals. most of the research on the mule superfamily has focused on plant mules where they were discovered and where some are extremely active and have significant impact on genome structure. the maize mudr element has been widely used as a tool for both forward and reverse genetic studies because of its high transposition rate and preference for targeting genic regions. however, despite being widesprea ...201728096902
juvenile hormone biosynthesis in adult blattella germanica requires nuclear receptors seven-up and ftz-f1.in insects, the transition from juvenile development to the adult stage is controlled by juvenile hormone (jh) synthesized from the corpora allata (ca) glands. whereas a jh-free period during the last juvenile instar triggers metamorphosis and the end of the growth period, the reappearance of this hormone after the imaginal molt marks the onset of reproductive adulthood. despite the importance of such transition, the regulatory mechanism that controls it remains mostly unknown. here, using the h ...201728074850
emergence of epidemic zika virus transmission and congenital zika syndrome: are recently evolved traits to blame?the mechanisms responsible for the dramatic emergence of zika virus (zikv), accompanied by congenital zika syndrome and guillain-barré syndrome (gbs), remain unclear. however, two hypotheses are prominent: (i) evolution for enhanced urban transmission via adaptation to mosquito vectors, or for enhanced human infection to increase amplification, or (ii) the stochastic introduction of zikv into large, naive human populations in regions with abundant aedes aegypti populations, leading to enough rar ...201728074023
fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression.diapause, also known as dormancy, is a state of arrested development that allows insects to survive unfavorable environmental conditions. diapause-destined insects store large amounts of fat when preparing for diapause. however, the extent to which these accumulated fat reserves influence diapause remains unclear. to address this question, we investigated the function of fatty acid synthase (fas), which plays a central role in lipid synthesis, in stress tolerance, the duration of diapause prepar ...201728071706
dengue in latin america: systematic review of molecular epidemiological trends.dengue, the predominant arthropod-borne viral disease affecting humans, is caused by one of four distinct serotypes (denv-1, -2, -3 or -4). a literature analysis and review was undertaken to describe the molecular epidemiological trends in dengue disease and the knowledge generated in specific molecular topics in latin america, including the caribbean islands, from 2000 to 2013 in the context of regional trends in order to identify gaps in molecular epidemiological knowledge and future research ...201728068335
proteomic analysis of cry2aa-binding proteins and their receptor function in spodoptera exigua.the bacterium bacillus thuringiensis produces crystal (cry) proteins that are toxic to a diverse range of insects. transgenic crops that produce bt cry proteins are grown worldwide because of their improved resistance to insect pests. although bt "pyramid" cotton that produces both cry1a and cry2a is predicted to be more resistant to several lepidopteran pests, including spodoptera exigua, than plants that produce cry1ac alone, the mechanisms responsible for the toxicity of cry2aa in s. exigua a ...201728067269
wolbachia infections in aedes aegypti differ markedly in their response to cyclical heat stress.aedes aegypti mosquitoes infected with wolbachia bacteria are currently being released for arbovirus suppression around the world. their potential to invade populations and persist will depend on interactions with environmental conditions, particularly as larvae are often exposed to fluctuating and extreme temperatures in the field. we reared ae. aegypti larvae infected with different types of wolbachia (wmel, walbb and wmelpop-cla) under diurnal cyclical temperatures. rearing wmel and wmelpop-c ...201728056065
the dangers of accepting a single diagnosis: case report of concurrent plasmodium knowlesi malaria and dengue infection.dengue and malaria are two common, mosquito-borne infections, which may lead to mortality if not managed properly. concurrent infections of dengue and malaria are rare due to the different habitats of its vectors and activities of different carrier mosquitoes. the first case reported was in 2005. since then, several concurrent infections have been reported between the dengue virus (denv) and the malaria protozoans, plasmodium falciparum and plasmodium vivax. symptoms of each infection may be mas ...201728049485
pterodon emarginatus oleoresin-based nanoemulsion as a promising tool for culex quinquefasciatus (diptera: culicidae) control.preparation of nanoformulations using natural products as bioactive substances is considered very promising for innovative larvicidal agents. on this context, oil in water nanoemulsions develop a main role, since they satisfactorily disperse poor-water soluble substances, such as herbal oils, in aqueous media. pterodon emarginatus, popularly known as sucupira, has a promising bioactive oleoresin. however, to our knowledge, no previous studies were carried out to evaluate its potential against cu ...201728049483
the physical genome mapping of anopheles albimanus corrected scaffold misassemblies and identified interarm rearrangements in genus anopheles.the genome of the neotropical malaria vector anopheles albimanus was sequenced as part of the 16 anopheles genomes project published in 2015. the draft assembly of this species consisted of 204 scaffolds with an n50 scaffold size of 18.1 mb and a total assembly size of 170.5 mb. it was among the smallest genomes with the longest scaffolds in the 16 anopheles species cluster, making an. albimanus the logical choice for anchoring the genome assembly to chromosomes. in this study, we developed a hi ...201727821634
a large scale biorational approach using bacillus thuringiensis israeliensis (strain am65-52) for managing aedes aegypti populations to prevent dengue, chikungunya and zika transmission.aedes aegypti is a container-inhabiting mosquito and a vector of dengue, chikungunya, and zika viruses. in 2009 several cases of autochthonous dengue transmission were reported in key west, florida, usa prompting a comprehensive response to control a. aegypti. in key west, larvae of this mosquito develop in containers around human habitations which can be numerous and labor intensive to find and treat. aerial applications of larvicide covering large areas in a short time can be an efficient and ...201728199323
levels of insecticide resistance to deltamethrin, malathion, and temephos, and associated mechanisms in aedes aegypti mosquitoes from the guadeloupe and saint martin islands (french west indies).in the guadeloupe and saint martin islands, aedes aegypti mosquitoes are the only recognized vectors of dengue, chikungunya, and zika viruses. for around 40 years, malathion was used as a mosquito adulticide and temephos as a larvicide. since the european union banned the use of these two insecticide molecules in the first decade of the 21st century, deltamethrin and bacillus thuringiensis var. israelensis are the remaining adulticide and larvicide, respectively, used in guadeloupe. in order to ...201728187780
response of three cyprinid fish species to the scavenger deterrent factor produced by the mutualistic bacteria associated with entomopathogenic nematodes.the symbiotic bacteria, photorhabdus and xenorhabdus associated with entomopathogenic nematodes (epns) in the genera heterorhabditis and steinernema, respectively, produce a compound(s) called the scavenging deterrent factor (sdf). sdf deters a number of terrestrial insect scavengers and predators and one bird species from feeding on host insects killed by the nematode-bacterium complex but has not been tested against aquatic vertebrates. moreover, the heterorhabditis-photorhabdus association is ...201727908637
entomological investigations during early stages of a chikungunya outbreak in the united states virgin islands, 2014.during the 2014 chikungunya (chik) outbreak in the caribbean, we performed entomological surveys on 3 united states virgin islands (usvi): st. croix, st. thomas, and st. john. we aimed to evaluate the potential for chikungunya virus (chikv) transmission in the usvi. the surveys took place between june 19, 2014, and june 29, 2014, during the dry season in usvi. a total of 1,929 adult mosquitoes belonging to 4 species- culex quinquefasciatus (68.4%), aedes aegypti (29.7%), ae. mediovittatus (1.3%) ...201728388320
potential of aedes aegypti and aedes albopictus populations in the central african republic to transmit enzootic chikungunya virus strains.major chikungunya outbreaks have affected several central african countries during the past decade. the chikungunya virus (chikv) was isolated from humans and sylvan mosquitoes in the central african republic (car) during the 1970 and 1980s but has not been found recently, despite the presence of aedes albopictus since 2010. the risk of a massive chikungunya epidemic is therefore potentially high, as the human populations are immunologically naïve and because of the presence of the mosquito vect ...201728347325
computer-aided structure based drug design approaches for the discovery of new anti-chikv agents.chikungunya is a viral infection caused by chikungunya virus (chikv), an arbovirus transmitted through mosquito (aedes aegypti and aedes albopictus) bite. the virus from sylvatic cycle in africa mutated to new vector adaptation and became one of the major emerging and re-emerging viral infections in the past decade, affecting more than 40 countries. efforts are being made by many researches to develop means to prevent and control the infection through vaccines and vector control strategy. on the ...201728294048
chikungunya infection: a global public health menace.chikungunya virus (chikv) has been involved in epidemics in african and asian subcontinents and, of late, has transcended to affect the americas. aedes aegypti and aedes albopictus are the major vectors for chikv infection, which results in dissemination of virus to various vital organs. entry of virus into these tissues causes infiltration of innate immune cells, monocytes, macrophages, neutrophils, natural killer cells, and adaptive immune cells. macrophages bearing the replicating virus, in t ...201728233156
aedes-borne virus-mosquito interactions: mass spectrometry strategies and findings.aedes-borne viruses are responsible for high-impact neglected tropical diseases and unpredictable outbreaks such as the ongoing zika epidemics. aedes mosquitoes spread different arboviruses such as dengue virus (denv), chikungunya virus (chikv), and zika virus, among others, and are responsible for the continuous emergence and reemergence of these pathogens. these viruses have complex transmission cycles that include two hosts, namely the aedes mosquito as a vector and susceptible vertebrate hos ...201728192064
environmental and social change drive the explosive emergence of zika virus in the americas.since zika virus (zikv) was detected in brazil in 2015, it has spread explosively across the americas and has been linked to increased incidence of microcephaly and guillain-barré syndrome (gbs). in one year, it has infected over 500,000 people (suspected and confirmed cases) in 40 countries and territories in the americas. along with recent epidemics of dengue (denv) and chikungunya virus (chikv), which are also transmitted by aedes aegypti and ae. albopictus mosquitoes, the emergence of zikv s ...201728182667
severe chikungunya infection in northern mozambique: a case report.although chikungunya virus has rapidly expanded to several countries in sub-saharan africa, little attention has been paid to its control and management. until recently, chikungunya has been regarded as a benign and self-limiting disease. in this report we describe the first case of severe chikungunya disease in an adult patient in pemba, mozambique.201728179029
transcriptome analysis of aedes aegypti in response to mono-infections and co-infections of dengue virus-2 and chikungunya virus.chikungunya virus (chikv) and dengue virus (denv) spread via the bite of infected aedes mosquitoes. both these viruses exist as co-infections in the host as well as the vector and are known to exploit their cellular machinery for their replication. while there are studies reporting the changes in aedes transcriptome when infected with denv and chikv individually, the effect both these viruses have on the mosquitoes when present as co-infections is not clearly understood. in the present study, we ...201728161634
chikungunya virus: an update on the biology and pathogenesis of this emerging pathogen.re-emergence of chikungunya virus, a mosquito-transmitted pathogen, is of serious public health concern. in the past 15 years, after decades of infrequent, sporadic outbreaks, the virus has caused major epidemic outbreaks in africa, asia, the indian ocean, and more recently the caribbean and the americas. chikungunya virus is mainly transmitted by aedes aegypti mosquitoes in tropical and subtropical regions, but the potential exists for further spread because of genetic adaptation of the virus t ...201728159534
engineered aedes aegypti jak/stat pathway-mediated immunity to dengue virus.we have developed genetically modified ae. aegypti mosquitoes that activate the conserved antiviral jak/stat pathway in the fat body tissue, by overexpressing either the receptor dome or the janus kinase hop by the blood feeding-induced vitellogenin (vg) promoter. transgene expression inhibits infection with several dengue virus (denv) serotypes in the midgut as well as systemically and in the salivary glands. the impact of the transgenes dome and hop on mosquito longevity was minimal, but it re ...201728081143
identification and initial characterization of matrix metalloproteinases in the yellow fever mosquito, aedes aegypti.aedes aegypti is a major vector for arboviruses such as dengue, chikungunya and zika viruses. during acquisition of a viremic bloodmeal, an arbovirus infects mosquito midgut cells before disseminating to secondary tissues, including the salivary glands. once virus is released into the salivary ducts it can be transmitted to another vertebrate host. the midgut is surrounded by a basal lamina (bl) in the extracellular matrix, consisting of a proteinaceous mesh composed of collagen iv and laminin. ...201728054419
[chikungunya, an emerging viral disease. proposal of an algorithm for its clinical management].chikungunya fever (chik) is an emerging viral disease. it is caused by the chikungunya virus, an alphavirus from the togaviridae family. it is transmitted to humans by the bite of infected mosquitoes, mainly aedes aegypti and aedes albopictus. they are also involved in the transmission of dengue, malaria, etc. chik is now endemic in any region of africa and southeast-asia. cases of chik have been reported in america, the caribbean, and europe (france, italy and spain). there are reservoirs of th ...201725440971
[technical guidelines for the prevention and treatment of chikungunya fever].chikungunya fever is an emerging disease caused by an alphavirus belonging to the togaviridae family, transmitted by the bite of aedes genus species: aedesaegypti and aedesalbopictus. in 2013, paho/who received confirmation of the first cases of indigenous transmission of chikungunya in the americas. this disease may be acute, subacute and chronic, affecting all age groups. following an incubation period from three to seven days, the patient usually begins with a high fever (greater than 39 °c), ...201725680650
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