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retention of duplicated long-wavelength opsins in mosquito lineages by positive selection and differential expression.opsins are light sensitive receptors associated with visual processes. insects typically possess opsins that are stimulated by ultraviolet, short and long wavelength (lw) radiation. six putative lw-sensitive opsins predicted in the yellow fever mosquito, aedes aegypti and malaria mosquito, anopheles gambiae, and eight in the southern house mosquito, culex quinquefasciatus, suggest gene expansion in the family culicidae (mosquitoes) relative to other insects. here we report the first detailed mol ...201728320313
frequency of aedes sp. linnaeus (diptera: culicidae) and associated entomofauna in bromeliads from a forest patch within a densely urbanized area.little is known about the relationship between the presence of aedes, abiotic factors and the entomofauna existing in phytotelmatas. the aim of this study was to identify biotic and abiotic factors associated with the presence of aedes in bromeliads sites located in a forest fragment in rio de janeiro, brazil. in the study area, eight bromeliads from the aechmea genus were chosen and kept in landscape form. physical and chemical variables were measured. collected aquatic insects were identified ...201728315189
a dynamic survival detection and analysis system for mosquito larvae viability in drug assays.bioinformatics studies have emerged in the domain of larval behavior analysis in recent years. a dynamic survival detection and analysis system for automatically monitoring a large amount of mosquito larvae in bioassays with multiwell plates by acquiring and processing videos is proposed in this article. in our system, equipment is designed for acquiring the video of the mosquito larvae in several multiwell plates simultaneously by a camera, and a video analysis module is developed for detecting ...201728314109
toxicity of spinosad to temephos-resistant aedes aegypti populations in brazil.the mosquito aedes aegypti is the primary vector of different arboviruses and represents a major public health problem. several brazilian populations of ae. aegypti have developed resistance to temephos, the most used organophosphate larvicide. new tools which are less harmful to the environment and safer for humans are becoming increasingly important to control this insect vector. spinosad, an aerobic fermentation product of a soil actinobacteria, has a favorable environmental profile. it prese ...201728301568
evaluation of reference genes at different developmental stages for quantitative real-time pcr in aedes aegypti.the mosquito aedes aegypti (ae. aegypti) is the most notorious vector of illness-causing viruses such as dengue, chikugunya, and zika. although numerous genetic expression studies utilizing quantitative real-time pcr (qpcr) have been conducted with regards to ae. aegypti, a panel of genes to be used suitably as references for the purpose of expression-level normalization within this epidemiologically important insect is presently lacking. here, the usability of seven widely-utilized reference ge ...201728300076
longitudinal study of thai people media exposure, knowledge, and behavior on dengue fever prevention and control.dengue hemorrhagic fever is transmitted through a bite by a dengue -infected aedes aegypti mosquito. it was first reported in the mid -20th century in thailand, and since then its epidemiology has been of great concern and has spread all across the country. the alarming incidence of dengue posed a serious threat to human health in all major cities of thailand. this study was aimed at identifying the level of awareness of dengue fever in thai population knowledge for prevention and control, and m ...201728285970
repellency of the origanum onites l. essential oil and constituents to the lone star tick and yellow fever mosquito.the oregano, origanum onites l., essential oil (eo) was tested in laboratory behavioural bioassays for repellent activity against amblyomma americanum (l.) and aedes aegypti (l.). the o. onites eo was characterised using gc-fid and gc-ms. carvacrol (75.70%), linalool (9.0%), p-cymene (4.33%) and thymol (1.9%) were the most abundant compounds. at a concentration of 0.413 mg oil/cm(2) of filter paper, o. onites eo repelled 100% of the ticks tested and at 0.103 mg oil/cm(2) of filter paper, 66.7% o ...201728278656
mosquito-borne diseases and omics: salivary gland proteome of the female aedes aegypti mosquito.the female aedes aegypti mosquito is an important vector for several tropical and subtropical diseases such as dengue, chikungunya, and zika and yellow fever. the disease viruses infect the mosquito and subsequently spread to the salivary glands after which the viruses can be transmitted to humans with probing or feeding by the mosquito. omics systems sciences offer the opportunity to characterize vectors and can inform disease surveillance, vector control and development of innovative diagnosti ...201728271980
cre/lox-recombinase-mediated cassette exchange for reversible site-specific genomic targeting of the disease vector, aedes aegypti.site-specific genome modification (ssm) is an important tool for mosquito functional genomics and comparative gene expression studies, which contribute to a better understanding of mosquito biology and are thus a key to finding new strategies to eliminate vector-borne diseases. moreover, it allows for the creation of advanced transgenic strains for vector control programs. ssm circumvents the drawbacks of transposon-mediated transgenesis, where random transgene integration into the host genome r ...201728266580
chemicals isolated from justicia adhatoda linn reduce fitness of the mosquito, aedes aegypti l.extracts from justicia adhatoda l. (acanthaceae) strongly reduced the fitness of the mosquito, aedes aegypti linn. the methanolic extracts inhibited several enzymes responsible for protecting insects from oxidative and other damage, including glutathione-s-transferase, superoxide dismutase, cytochrome p450, and α- and β-esterases. they increased repellency (maximum repellency at 100 ppm) in host-seeking adult females using the "arm-in cage assay." histopathological examination showed the extract ...201728266058
comparative luciferase assay for establishing reliable in vitro screening system of juvenile hormone agonists.the cultured cell-based in vitro assay using the stringency of ligand-receptor interactions is typically useful for screening certain hormone agonists from among a very large number of molecules. however, ligands are frequently altered or modified through evolution; indeed, even in the same receptor orthologs, different ligand sensitivity profiles are considered to arise among species and/or taxa. such ligand transition has been observed in juvenile hormone (jh), one of the most important endocr ...201728261832
blood feeding status, gonotrophic cycle and survivorship of aedes (stegomyia) aegypti (l.) (diptera: culicidae) caught in churches from merida, yucatan, mexico.blood-feeding status, gonotrophic cycle, and survival rates of aedes (stegmyia) aegypti (l.) was investigated in catholic churches from merida, yucatan. female ae. aegypti were caught using backpack aspirator during 25 consecutive days in rainy (2015) and dry season (2016). blood-feeding status was determined by external examination of the abdomen and classified as unfed, fed, and gravid. daily changes in the parous-nulliparous ratio were recorded, and the gonotrophic cycle length was estimated ...201728258352
insecticide susceptibility status and major detoxifying enzymes' activity in aedes albopictus (skuse), vector of dengue and chikungunya in northern part of west bengal, india.mosquitoes belonging to aedes genus, aedes aegypti and aedes albopictus transmit many globally important arboviruses including dengue (denv) and chikungunya (chikv). vector control with the use of insecticide remains the suitable method of choice to stop the transmission of these diseases. however, vector control throughout the world is failing to achieve its target results because of the worldwide development of insecticide resistance in mosquitoes. to assess the insecticide susceptibility stat ...201728254583
a mesh-duox pathway regulates homeostasis in the insect gut.the metazoan gut harbours complex communities of commensal and symbiotic bacterial microorganisms. the quantity and quality of these microorganisms fluctuate dynamically in response to physiological changes. the mechanisms that hosts have developed to respond to and manage such dynamic changes and maintain homeostasis remain largely unknown. here, we identify a dual oxidase (duox)-regulating pathway that contributes to maintaining homeostasis in the gut of both aedes aegypti and drosophila melan ...201728248301
allergenome characterization of the mosquito aedes aegypti.saliva and muscle derived mosquito allergens have been purified and characterized. however, the complete set of allergens remains to be elucidated. in this study, we identified and characterized ige-binding proteins from the mosquito species a. aegypti.201728235135
characterization of carbonic anhydrase 9 in the alimentary canal of aedes aegypti and its relationship to homologous mosquito carbonic anhydrases.in the mosquito midgut, luminal ph regulation and cellular ion transport processes are important for the digestion of food and maintenance of cellular homeostasis. ph regulation in the mosquito gut is affected by the vectorial movement of the principal ions including bicarbonate/carbonate and protons. as in all metazoans, mosquitoes employ the product of aerobic metabolism carbon dioxide in its bicarbonate/carbonate form as one of the major buffers of cellular and extracellular ph. the conversio ...201728230813
relish2 mediates bursicon homodimer-induced prophylactic immunity in the mosquito aedes aegypti.bursicon is a neuropeptide hormone consisting of two cystine-knot proteins (burs α and burs β), responsible for cuticle tanning and other developmental processes in insects. recent studies show that each bursicon subunit forms homodimers that induce prophylactic immunity in drosophila melanogaster. here, we investigated the hypothesis that bursicon homodimers act in prophylactic immunity in insects, and possibly arthropods, generally, using the mosquito, aedes aegypti. we found that burs α and b ...201728225068
transcriptome-wide microrna and target dynamics in the fat body during the gonadotrophic cycle of aedes aegypti.the mosquito aedes aegypti is a major vector of numerous viral diseases, because it requires a blood meal to facilitate egg development. the fat body, a counterpart of mammalian liver and adipose tissues, is the metabolic center, playing a key role in reproduction. therefore, understanding of regulatory networks controlling its functions is critical, and the role of micrornas (mirnas) in the process is largely unknown. we aimed to explore mirna expression and potential targets in the female fat ...201728223504
regulation of antimicrobial peptides in aedes aegypti aag2 cells.antimicrobial peptides (amps) are an important group of immune effectors that play a role in combating microbial infections in invertebrates. most of the current information on the regulation of insect amps in microbial infection have been gained from drosophila, and their regulation in other insects are still not completely understood. here, we generated an amp induction profile in response to infections with some gram-negative, -positive bacteria, and fungi in aedes aegypti embryonic aag2 cell ...201728217557
comparative analysis of mosquito (diptera: culicidae: aedes aegypti liston) responses to the insecticide temephos and plant derived essential oil derived from piper betle l.resistance to treatments with temephos or plant derived oil, pb-cvo, between a field collected wild strain (ws) and a susceptible laboratory strain (ls) of ae. aegypti were measured. the temephos (0.1mg/l) showed the greatest percentage of mosquito mortality compared to pb-cvo (1.5mg/l) in ls ae. aegypti. however, ws ae. aegypti was not significantly affected by temephos (0.1mg/l) treatment compare to the pb-cvo (1.5mg/l). however, both strains (ls and ws) when treated with pb-cvo (1.5mg/l) disp ...201728213320
household dengue prevention interventions, expenditures, and barriers to aedes aegypti control in machala, ecuador.the aedes aegypti mosquito is an efficient vector for the transmission of zika, chikungunya, and dengue viruses, causing major epidemics and a significant social and economic burden throughout the tropics and subtropics. the primary means of preventing these diseases is household-level mosquito control. however, relatively little is known about the economic burden of ae. aegypti control in resource-limited communities. we surveyed residents from 40 households in a high-risk community at the urba ...201728212349
environmental factors can influence dengue reported cases.global climate changes directly affect the natural environment and contribute to an increase in the transmission of diseases by vectors. among these diseases, dengue is at the top of the list. the aim of our study was to understand the consequences of temporal variability of air temperature in the occurrence of dengue in an area comprising seven municipalities of the greater são paulo.201729451659
[(assessment of risk for arbovirus infections in russia)].the sharply increased incidence and geographical expansion of arbovirus diseases in recent years suggest that some russian areas are vulnerable to vector-borne diseases and that it is important to elaborate control programs to ensure bio- logical safety in our country. moreover, the main vectors of the pathogens of arbovirus infections (zika fever, dengue fever, chikungunya fevers), such as aedes aegypti and ae.albopictus, have been registered on the black sea coast of the caucasus. the programs ...201730721608
larvicidal and pupicidal activities of crude and fractionated extracts of acacia pennata (l.) willd. subsp insuavis shoot tips against aedes aegypti (l.) (diptera: culicidae).acacia pennata subsp insuavis, or cha-om in thai, is a common vegetablefound in thailand. it has been used as a medicinal herb for a long time. from theliterature, antinociceptive, anti-inflammatory, antimicrobial, and anti-helminthicactivities were reported. in this study, we investigated two new actions of thisplant: larvicide and pupicide. the crude ethanolic and fractionated extracts of a.pennata shoot tips were tested against aquatic stages of the dengue virus vector,aedes aegypti mosquito. ...201729644817
infectivity of chordodes nobilii larvae (gordiida: nematomorpha).the gordiids are freshwater representatives of the parasite phylum nematomorpha that function asa link between aquatic and terrestrial ecosystems. in recent years, different ecotoxicologic studies have beenmade with the south-american gordiid species, chordodes nobilii, that have demonstrated the capacity of thisgroup to act as a bioindicator of contamination. despite the gordiida’s ecologic relevance, further studies arestill needed to elucidate different aspects of the biology of the class, an ...201729465953
[clinical and epidemiological profile of chikungunya infection in hospital patients in 2015 in mérida, mexicoperfil clínico-epidemiológico da infecção por chikungunya em casos hospitalares atendidos em 2015 em mérida, méxico].describe the epidemiological profile and analyze clinical manifestations by age group in chikungunya patients treated in 2015 in a mérida, mexico general hospital.201731391823
erratum: efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae). 201728365772
tropical viruses approaching europe.during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ...201729952164
tropical viruses approaching europe.during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ...201729952163
evaluation of phytosynthesised silver nanoparticles from leaf extracts of leucas aspera and hyptis suaveolens and their larvicidal activity against malaria, dengue and filariasis vectors.the present study deals with the green synthesis of silver nanoparticle from the aqueous leaf extracts of leucas aspera and hyptis suaveolens as reducing agent and to investigate the larvicidal activity of synthesized silver nanoparticles. the synthesized silver nanoparticles were characterized by ultraviolet and visible absorption spectroscopy (uv), fourier transform-infrared spectroscopy (ft-ir), x-ray spectroscopy (xrd), field emission scanning electron microscope (fesem) and high-resonance t ...201729774292
biological control of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps.mosquitoes can act as vectors of important diseases such as malaria, dengue, zika virus, yellow fever, chikungunya and mayaro fever, in addition to filariasis. the use of insecticides, larvicides, bed nets and repellents, besides the use of drugs as chemoprevention and the treatment of the sick are currently the pillars of the control of these vectors. we studied the biological control against of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps of the species m. ...201729774286
fly ash-based water dispersible powder formulation of bacillus thuringiensis var. israelensis: development & laboratory evaluation against mosquito immatures.bacillus thuringiensis var. israelensis (bti) formulations are presently being used for insect control. in this study, a water dispersible powder (wdp) formulation using fly ash (fa) as a carrier material was developed and studied for its activity against the larval stages of major mosquito vector species.201729664029
ultrasound is not useful as a screening tool for dengue fever.dengue fever is a tropical disease that is transmitted by female aedes aegypti mosquitos. early diagnosis is necessary to reduce the mortality and morbidity associated with the disease. a combination of clinical, laboratory, and sonography findings can be potentially helpful in making an early diagnosis of dengue fever. there is paucity of literature on the use of ultrasound for dengue fever screening; hence, the primary objective of the study was to evaluate the utility of ultrasound as a scree ...201729657635
estimating the size of aedes aegypti populations from dengue incidence data: implications for the risk of yellow fever outbreaks.in this paper we present a model to estimate the density of aedes mosquitoes in a community affected by dengue. the method consists in fitting a continuous function to the incidence of dengue infections, from which the density of infected mosquitoes is derived straightforwardly. further derivations allow the calculation of the latent and susceptible mosquitoes' densities, the sum of the three equals the total mosquitoes' density. the method is illustrated with the case of the risk of urban yello ...201730137722
evaluation of standard field and laboratory methods to compare protection times of the topical repellents pmd and deet.mosquitoes are important vectors of pathogens, and travellers to disease endemic countries are advised to avoid bites by applying topical repellents. topical repellents are typically tested either in the arm-in-cage (aic) test under laboratory conditions or in the field, but not often under both conditions. we, therefore, investigated how two topical repellents, 15% para-menthane-3,8-diol (pmd) and 15% n,n-diethyl-3-methylbenzamide (deet) compare against each other both in the aic test against t ...201830135603
experimental evaluation of the impact of household aerosolized insecticides on pyrethroid resistant aedes aegypti.the extensive reliance on insecticides to control aedes aegypti mosquitoes and disrupt transmission of dengue, chikungunya and zika has fueled the emergence of widespread resistance to insecticides. mismatch between the frequency of pyrethroid resistance in mosquitoes and the occurrence of pyrethroid-based insecticide applications for vector control is often hypothesized to be due to household use of commercial insecticide products. we experimentally quantified phenotypic and genotypic responses ...201830135460
differential transmission of antiviral drug-resistant chikungunya viruses by aedes mosquitoes.the chikungunya virus (chikv) is transmitted by female aedes aegypti and aedes albopictus mosquitoes, mostly present in (sub)tropical regions. no antivirals are available to treat chikv infections. if antiviral drugs are proven efficient to treat chikv-infected patients, it will be pivotal to determine whether drug-resistant viruses can be transmitted from one human to another by their mosquito vectors. we orally infected aedes aegypti mosquitoes with a blood meal containing wild-type or drug-re ...201830135220
study of aedes aegypti population with emphasis on the gonotrophic cycle length and identification of arboviruses: implications for vector management in cemeteries.aedes aegypti is the vector of the arboviruses causing dengue, chikungunya and zika infections in mexico. however, its presence in public places has not been fully evaluated. in a cemetery from merida, yucatan, mexico, the productivity of ae. aegypti, the gonotrophic cycle, and the presence of ae. aegypti females infected with arboviruses were evaluated. immature and adult mosquitoes were inspected every two months between april 2016 to june 2017. for the gonotrophic cycle length, the daily patt ...201830133604
estimating the effects of variation in viremia on mosquito susceptibility, infectiousness, and r0 of zika in aedes aegypti.zika virus (zikv) is an arbovirus primarily transmitted by aedes mosquitoes. like most viral infections, zikv viremia varies over several orders of magnitude, with unknown consequences for transmission. to determine the effect of viral concentration on zikv transmission risk, we exposed field-derived ae. aegypti mosquitoes to four doses (10(3), 10(4), 10(5), 10(6) pfu/ml) representative of potential variation in the field. we demonstrate that increasing zikv dose in the blood-meal significantly ...201830133450
estimating air temperature using modis surface temperature images for assessing aedes aegypti thermal niche in bangkok, thailand.dengue, the most widespread urban vector-borne disease, is transmitted to human by the mosquito aedes aegypti. its distribution in urban areas is heterogeneous over time and space. in time, it is linked to seasonal variations such as warm and cold seasons, as well as rainy and dry seasons. in space, it is linked to social and environmental conditions, which alternate between rich and deprived neighborhoods, vegetated and densely built areas. these variations in terms of land cover can affect sur ...201830132225
efficacy of culicinomyces spp. against aedes aegypti eggs, larvae and adults.the aquatic fungal genus culicinomyces attacks dipteran larvae but little is known about its efficacy against aedes aegypti. here we report on the activity of both described species-culicinomyces clavisporus and culicinomyces bisporalis-on larvae, eggs and adults, and on trans-stadial transmission. ten c. clavisporus isolates (arsef 372, 582, 644, 706, 964, 1260, 2471, 2478, 2479 and 2480) and c. bisporalis arsef 1948 were screened against larvae of this important vector of viral diseases. arsef ...201830130538
glyphosate impairs learning in aedes aegypti mosquito larvae at field-realistic doses.glyphosate is the most widely used herbicide in the world. over the past few years, the number of studies revealing deleterious effects of glyphosate on non-target species has been increasing. here, we studied the impact of glyphosate at field-realistic doses on learning in mosquito larvae (aedes aegypti). larvae of a. aegypti live in small bodies of water and perform a stereotyped escape response when a moving object projects its shadow on the water surface. repeated presentations of an innocuo ...201830127074
laboratory selection of aedes aegypti field populations with the organophosphate malathion: negative impacts on resistance to deltamethrin and to the organophosphate temephos.resistance to pyrethroids and to the organophosphate temephos is widespread in brazilian populations of the dengue vector, aedes aegypti. thereof, since 2009 insect growth regulators are employed as larvicides, and malathion is used against adults.201830125295
travel-associated zika cases and threat of local transmission during global outbreak, california, usa.zika and associated microcephaly among newborns were reported in brazil during 2015. zika has since spread across the americas, and travel-associated cases were reported throughout the united states. we reviewed travel-associated zika cases in california to assess the potential threat of local zika virus transmission, given the regional spread of aedes aegypti and ae. albopictus mosquitoes. during november 2015-september 2017, a total of 588 travel-associated zika cases were reported in californ ...201830124194
chicory (cichorium intybus) and wormwood (artemisia absinthium) extracts exhibit strong larvicidal activity against mosquito vectors of malaria, dengue fever, and filariasis.vector-borne diseases transmitted by mosquitoes cause globally important diseases such as malaria, dengue fever, and filariasis. the incidence of these diseases can be reduced through mosquito control programs but these control programs currently rely on synthetic insecticides that can impact the environment, and has selected widespread mosquito resistance. environment friendly and biodegradable natural insecticides discovered in plants offer an alternative approach to mosquito control. here, we ...201830118862
colonized sabethes cyaneus, a sylvatic new world mosquito species, shows a low vector competence for zika virus relative to aedes aegypti.the introduction of zika virus (zikv) to the americas raised concern that the virus would spill back from human transmission, perpetuated by aedes aegypti, into a sylvatic cycle maintained in wildlife and forest-living mosquitoes. in the americas, sabethes species are vectors of sylvatic yellow fever virus (yfv) and are therefore candidate vectors of a sylvatic zikv cycle. to test the potential of sabethes cyaneus to transmit zikv, sa. cyaneus and ae. aegypti were fed on a129 mice one or two day ...201830115888
levels of resistance to pyrethroid among distinct kdr alleles in aedes aegypti laboratory lines and frequency of kdr alleles in 27 natural populations from rio de janeiro, brazil.several mutations in voltage gated sodium channel (nav) have been identified in aedes aegypti populations worldwide. however, only few are related to knockdown resistance to pyrethroids, most of which with variations in the 1016 and 1534 nav sites. in brazil, at least two nav alleles are known: navr1, with a substitution in the 1534 (1016 val+ + 1534 ile kdr ) and navr2, with substitutions in both 1016 and sites (1016ilekdr + 1534cys kdr ). there is also the duplication in the nav gene, with one ...201830112367
oviplate: a convenient and space-saving method to perform individual oviposition assays in aedes aegypti.aedes aegypti is the principal vector of the urban arboviruses and the blood ingestion is important to produce the eggs in this species. to analyze the egg production in ae. aegypti, researchers frequently use small cages or drosophila vials to collect eggs from gravid females. although it is affordable, the setup is time- and space-consuming, mainly when many mosquitoes need to be individually analyzed. this study presents an easy, cheap, and space-saving method to perform individual ovipositio ...201830111702
temperature drives zika virus transmission: evidence from empirical and mathematical models.temperature is a strong driver of vector-borne disease transmission. yet, for emerging arboviruses we lack fundamental knowledge on the relationship between transmission and temperature. current models rely on the untested assumption that zika virus responds similarly to dengue virus, potentially limiting our ability to accurately predict the spread of zika. we conducted experiments to estimate the thermal performance of zika virus (zikv) in field-derived aedes aegypti across eight constant temp ...201830111605
zika might not be acting alone: using an ecological study approach to investigate potential co-acting risk factors for an unusual pattern of microcephaly in brazil.zika virus infections can cause a range of neurologic disorders including congenital microcephaly. however, while zika infections have been notified across all regions in brazil, there has been an unusual number of congenital microcephaly case notifications concentrated in the northeast of the country. to address this observation, we investigated epidemiological data (2014-2016) on arbovirus co-distribution, environmental and socio-economic factors for each region in brazil. data on arbovirus re ...201830110370
parental and offspring larval diets interact to influence life-history traits and infection with dengue virus in aedes aegypti.the environmental conditions experienced by parents can influence offspring phenotype along with the conditions experienced by offspring. these parental effects are clear in organisms that display parental care and are less clear in other organisms. here, we consider effects of parental and offspring larval nutrition on offspring development time, survivorship and infection with dengue virus in aedes aegypti, the mosquito vector of dengue, chikungunya, yellow fever and zika. parents were raised ...201830109101
anti-inflammatory activities of aedes aegypti cecropins and their protection against murine endotoxin shock.mosquitoes are armed with physiologically active compounds to suppress the host immunity including host inflammatory reaction. however, the specific anti-inflammatory components in mosquitoes remain unknown.201830107813
impedimetric immunosensor for dengue diagnosis using graphite screen-printed electrodes coated with poly(4-aminophenylacetic acid).dengue is an infectious viral disease transmitted by the aedes aegypti mosquito, the control of which is complex. in addition, the clinical diagnosis is difficult to perform since it resembles other febrile infections; thus, the development of more effective methods to detect dengue virus (dv) has drawn increasing attention. the present study aimed to develop an impedimetric immunosensor for dengue diagnosis using a screen-printed electrode (spe) functionalized with polymer films derived from 4- ...201830182296
dengue virus infection changes aedes aegypti oviposition olfactory preferences.aedes aegypti mosquitoes, main vectors for numerous flaviviruses, have olfactory preferences and are capable of olfactory learning especially when seeking their required environmental conditions to lay their eggs. in this study, we showed that semiochemical conditions during aedes aegypti larval rearing affected future female choice for oviposition: water-reared mosquitoes preferred to lay eggs in water or p-cresol containers, while skatole reared mosquitoes preferred skatole sites. using two in ...201830181545
a conceptual model for optimizing vaccine coverage to reduce vector-borne infections in the presence of antibody-dependent enhancement.many vector-borne diseases co-circulate, as the viruses from the same family are also transmitted by the same vector species. for example, zika and dengue viruses belong to the same flavivirus family and are primarily transmitted by a common mosquito species aedes aegypti. zika outbreaks have also commonly occurred in dengue-endemic areas, and co-circulation and co-infection of both viruses have been reported. as recent immunological cross-reactivity studies have confirmed that convalescent plas ...201830173664
demonstration of efficient vertical and venereal transmission of dengue virus type-2 in a genetically diverse laboratory strain of aedes aegypti.aedes aegypti is the primary mosquito vector of dengue viruses (denv; serotypes 1-4). human-mosquito transmission cycles maintain denv during epidemics but questions remain regarding how these viruses survive when human infections and vector abundance are minimal. aedes mosquitoes can transmit denv within the vector population through two alternate routes: vertical and venereal transmission (vt and vnt, respectively). we tested the efficiency of vt and vnt in a genetically diverse laboratory (gd ...201830169505
the surveillance of chikungunya virus in a temperate climate: challenges and possible solutions from the experience of lazio region, italy.chikv has become an emerging public health concern in the temperate regions of the northern hemisphere as a consequenceof the expansion of the endemic areas of its vectors (mainly aedes aegypti and aedesalbopictus). in 2017, a new outbreak of chikv was detected in italy with three clusters of autochthonous transmission in the lazio region (central italy), in the cities of anzio, rome, and latina and a secondary cluster in the calabria region (south italy). given the climate characteristics of it ...201830223536
where vectors collide: the importance of mechanisms shaping the realized niche for modeling ranges of invasive aedes mosquitoes.the vector mosquitoes aedes aegypti (l.), native to africa, and aedes albopictus (skuse), native to asia, are widespread invasives whose spatial distributions frequently overlap. predictive models of their distributions are typically correlative rather than mechanistic, and based on only abiotic variables describing putative environmental requirements despite extensive evidence of competitive interactions leading to displacements. here we review putative roles of competition contributing to dist ...201830220875
differences in the replicative capacities of clinical isolates of dengue virus in c6/36 cells and in urban populations of aedes aegypti from colombia, south america.dengue, the most prevalent arboviral disease worldwide, is caused by any of the four dengue virus (denv) serotypes that co-circulate constantly in hyperendemic areas such as medellin (colombia), and these serotypes are transmitted by mosquitoes of the genus aedes. in this study, we evaluated the replicative capacity of strains isolated in medellin between 2003 and 2007 in c6/36 cells and in colonies of aedes aegypti collected during 2010-2011 from high or low-incidence areas within the same city ...201830165044
direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of wolbachia using a cellphone.manipulation of natural mosquito populations using the endosymbiotic bacteria wolbachia is being investigated as a novel strategy to reduce the burden of mosquito-borne viruses. to evaluate the efficacy of these interventions, it will be critical to determine wolbachia infection frequencies in aedes aegypti mosquito populations. however, current diagnostic tools are not well-suited to fit this need. morphological methods cannot identify wolbachia, immunoassays often suffer from low sensitivity a ...201830161131
yellow fever in africa and the americas: a historical and epidemiological perspective.yellow fever was transported during the slave trade in the 15th and 16th centuries from africa to the americas where the virus encountered favorable ecological conditions that allowed creation of a sustainable sylvatic cycle. despite effective vector control and immunization programs for nearly a century, yellow fever epidemics reemerged in many latin american countries, particularly brazil. the emergence or reemergence of vector-borne diseases encompasses many intricate factors. yellow fever ou ...201830158957
local and regional dynamics of chikungunya virus transmission in colombia: the role of mismatched spatial heterogeneity.mathematical models of transmission dynamics are routinely fitted to epidemiological time series, which must inevitably be aggregated at some spatial scale. weekly case reports of chikungunya have been made available nationally for numerous countries in the western hemisphere since late 2013, and numerous models have made use of this data set for forecasting and inferential purposes. motivated by an abundance of literature suggesting that the transmission of this mosquito-borne pathogen is local ...201830157921
sublethal effects of a vapour-active pyrethroid, transfluthrin, on aedes aegypti and ae. albopictus (diptera: culicidae) fecundity and oviposition behaviour.it is assumed that mosquitoes surviving exposure to spatial repellents when attempting to bite a host will not have significant adverse impacts on their downstream biology. therefore, a critical knowledge gap is understanding the extent to which sublethal exposure to volatile pyrethroids may damage the performance of mosquitoes that survive exposure to vapour-active pyrethroids. to address this, laboratory-reared aedes aegypti (l.) and ae. albopictus (skuse) were exposed to one of three subletha ...201830157907
zika infection decreases aedes aegypti locomotor activity but does not influence egg production or viability.zika has emerged as a new public health threat after the explosive epidemic in brazil in 2015. it is an arbovirus transmitted mainly by aedes aegypti mosquitoes. the knowledge of physiological, behavioural and biological features in virus-infected vectors may help the understanding of arbovirus transmission dynamics and elucidate their influence in vector capacity.201830156598
oviposition deterrent efficacy and characteristics of a botanical natural product, ocimum gratissimum (l.) oil-alginate beads, against aedes aegypti (l.).this study was aimed at investigating the oviposition deterrent activity of ocimum gratissimum (l.) essential oil (o. gratissimum oil) and its product, ocimum gratissimum (l.)- alginate beads (beads), against aedes aegypti (ae. aegypti) mosquitoes. chemical analysis of o. gratissimum oil obtained by hydrodistillation, using gas chromatography-mass spectroscopy techniques, presented eugenol (67.38%) and z-β-ocimene (14.95 %) as major constituents. good characteristics of beads were obtained by th ...201830154681
forced salivation as a method to analyze vector competence of mosquitoes.vector competence is defined as the potential of a mosquito species to transmit a mosquito-borne virus (mobovirus) to a vertebrate host. viable virus particles are transmitted during a blood meal via the saliva of an infected mosquito. forced salivation assays allow determining the vector potential on the basis of single mosquitoes, avoiding the use of animal experiments. the method is suitable to analyze a large number of mosquitoes in one experiment within a short period of time. forced saliva ...201830148488
qsar modelling of synergists to increase the efficacy of deltamethrin against pyrethroid-resistant aedes aegypti mosquitoes$.space spraying of deltamethrin allows the control of adult aedes (stegomyia) aegypti mosquitoes. unfortunately, many vector control programs are threatened by the development of resistances that decrease the efficacy of this adulticide. faced with this situation, we can either try to use another insecticide presenting a different mechanism of action or find a strategy that brings back the efficacy of the insecticide at a satisfying level to pursue its use in vector control. restoration of the ef ...201830141356
transcriptomic analysis of aedes aegypti in response to mosquitocidal bacillus thuringiensis llp29 toxin.globally, aedes aegypti is one of the most dangerous mosquitoes that plays a crucial role as a vector for human diseases, such as yellow fever, dengue, and chikungunya. to identify (1) transcriptomic basis of midgut (2) key genes that are involved in the toxicity process by a comparative transcriptomic analysis between the control and bacillus thuringiensis (bt) toxin (llp29 proteins)-treated groups. next-generation sequencing technology was used to sequence the midgut transcriptome of a. aegypt ...201830140020
optimization of cationic (q)-paper for detection of arboviruses in infected mosquitoes.previously (glushakova et al. 2017), a cellulose-based cationic (q) paper derivatized with quaternary ammonium groups was shown to be a convenient platform to collect, preserve, and store nucleic acids (nas) derived from mosquito vectors infected with pathogens for surveillance. nas bind electrostatically to q-paper, but the quantity of na bound depends on the paper's binding capacity. to optimize the original technology for mosquito surveillance, factors that affected na absorbance on q-paper w ...201830099053
discovery and high prevalence of phasi charoen-like virus in field-captured aedes aegypti in south china.arboviruses have caused significant global health concerns during the past decade. in this regard, continuous viral surveillance is essential to timely identify emerging arboviruses and other novel viruses. here, a novel isolate of phasi charoen-like virus (pclv zhanjiang01) was identified from field-captured aedes aegypti mosquitoes in zhanjiang by next generation sequencing. phylogenetic analysis suggested that pclv zhanjiang01 belonged to the genus phasivirus in the family phenuiviridae. the ...201830077072
managing aedes aegypti populations in the first zika transmission zones in the continental united states.the african zika virus swept across the pacific, reaching the new world in 2014. in july, 2016, miami-dade county, florida became the locus of the first mosquito-borne zika transmission zones in the continental united states. control efforts were guided by the centers for disease control and prevention, including aerial and truck sprays of adulticides and larvicides. to improve our understanding of how best to fight zika transmission in an urban environment in the developed world, trap counts of ...201830075097
genetic characterization of chikungunya viruses isolated during the 2015-2017 outbreaks in different states of india, based on their e1 and e2 genes.during 2015-2017, chikungunya virus (chikv) showed a resurgence in several parts of india with karnataka, maharashtra and new delhi accounting for a majority of the cases. e2-e1 gene based characterization revealed indian subcontinent sublineage strains possessing aedes aegypti mosquito-adaptive mutations e1: k211e and e2:v264a, with the 211 site positively selected. novel mutational sites e1: k16e/q, e1: k132q/t, e1: s355t, e2: c19r and e2:s185y could be associated with epitopes or virulence de ...201830073419
a low-cost, battery-powered acoustic trap for surveilling male aedes aegypti during rear-and-release operations.the aedes aegypti mosquito is a primary vector of several serious arboviruses throughout the world and is therefore of great concern to many public health organizations. with vector control methodology pivoting towards rearing and releasing large numbers of genetically modified, sterilized, or wolbachia-infected male mosquitoes to control vector populations, economical surveillance methods for release tracking becomes increasingly necessary. previous work has identified that male ae. aegypti are ...201830071091
rapid evolution and the genomic consequences of selection against interspecific mating.while few species introduced into a new environment become invasive, those that do provide critical information on ecological mechanisms that determine invasions success and the evolutionary responses that follow invasion. aedes albopictus (the asian tiger mosquito) was introduced into the naturalized range of aedes aegypti (the yellow fever mosquito) in the united states in the mid-1980s, resulting in the displacement of a. aegypti in much of the south-eastern united states. the rapid displacem ...201830069966
small females prefer small males: size assortative mating in aedes aegypti mosquitoes.with aedes aegypti mosquitoes now being released in field programmes aimed at disease suppression, there is interest in identifying factors influencing the mating and invasion success of released mosquitoes. one factor that can increase release success is size: released males may benefit competitively from being larger than their field counterparts. however, there could be a risk in releasing only large males if small field females avoid these males and instead prefer small males. here we invest ...201830068363
a gustatory receptor used for rapid detection of tyrophagus putrescentiae in fungi hosts.the storage mite, tyrophagus putrescentiae, found worldwide in many habitats, is an important pest of edible fungi in china. storage mites are tiny and difficult to observe, especially when they occur in fungi composts. in this study, one gustatory receptor protein (tputgr1) was identified from the transcriptome of t. putrescentiae. phylogenetic analysis of grs families from 10 arthropod species revealed that tputgr1 had high homology with the sccagr1 of sarcoptes scabiei and turtgr1-2 of tetran ...201830061635
juvenile hormone-regulated alternative splicing of the taiman gene primes the ecdysteroid response in adult mosquitoes.juvenile hormone (jh) regulates many aspects of insect development and reproduction. in some processes, jh plays a critical role in defining the action of the steroid hormone 20-hydroxyecdysone (20e). in aedes aegypti mosquitoes, jh prepares newly emerged female adults to become competent to synthesize vitellogenin in response to 20e after blood ingestion. the molecular basis of this competence is still not well understood. here, we report that jh regulates pre-mrna splicing of the taiman gene, ...201830061397
comparing the effectiveness of different strains of wolbachia for controlling chikungunya, dengue fever, and zika.once aedes aegypti and aedes albopictus mosquitoes that spread chikungunya virus, dengue virus, and zika virus are infected with wolbachia, they have reduced egg laying rates, reduced transmission abilities, and shorter lifespans. since most infected mosquitoes are only infectious in the last few days of their lives, shortening a mosquito's lifespan by a day or two can greatly reduce their abilities to spread mosquito-borne viral diseases, such as chikungunya, dengue fever, and zika. we develope ...201830059498
modeling the importation and local transmission of vector-borne diseases in florida: the case of zika outbreak in 2016.chikungunya, dengue, and zika viruses are all transmitted by aedes aegypti and aedes albopictus mosquito species, had been imported to florida and caused local outbreaks. we propose a deterministic model to study the importation and local transmission of these mosquito-borne diseases. the purpose is to model and mimic the importation of these viruses to florida via travelers, local infections in domestic mosquitoes by imported travelers, and finally non-travel related transmissions to local huma ...201830053386
modifying the biogents sentinel trap to increase the longevity of captured aedes aegypti.aedes aegypti (l.) (diptera: culicidae) is a vector of viruses causing dengue, zika, chikungunya, and yellow fever and subsequently pose a significant global threat to public health. while sampling live mosquitoes is useful for surveillance purposes, most traps targeting aedes kill captured mosquitoes. the biogents sentinel (bgs) trap, the gold standard for capturing ae. aegypti, is one such trap. in our study, we modified the bgs trap to increase the survival of captured ae. aegypti by replacin ...201830053026
experimental study of the susceptibility of a european aedes albopictus strain to dengue virus under a simulated mediterranean temperature regime.dengue virus (denv) has re-emerged in europe driven by the geographic expansion of the mosquito species aedes albopictus and aedes aegypti (diptera: culicidae) and the introduction of the virus by viraemic travellers. in the present study, the vector competence (vc) of ae. albopictus collected in catalonia (northeast spain) was evaluated for two different denv strains, denv-1 and denv-2, the serotypes responsible for all outbreaks of dengue that have occurred in europe. mosquitoes were reared un ...201830051490
larvicidal effectiveness of azolla pinnata against aedes aegypti (diptera: culicidae) with its effects on larval morphology and visualization of behavioural response.dengue is vector-borne diseases with 390 million infections per year extending over 120 countries of the world. aedes aegypti (l.) (diptera: culicidae) is a primary vector for dengue viral infections for humans. current focus on application of natural product against mosquito vectors has been the main priority for research due to its eco-safety. the extensive use of chemical insecticides has led to severe health problems, environmental pollution, toxic hazards to human and nontarget species, and ...201830050688
the impact of the age of first blood meal and zika virus infection on aedes aegypti egg production and longevity.the impact of senescence and pathogen infection on aedes aegypti life-history traits remains poorly understood. this laboratory study focused on the impact of zika virus (zikv) infection and the age of first blood intake on blood meal and clutch sizes, and more importantly on the egg production ratio per μl of blood. three groups of zikv-infected and uninfected ae. aegypti females that received their first blood meal at 7 (young feeders), 14 (mature feeders) and 21 days old (old feeders) were mo ...201830048481
characterization of dengue virus in aedes aegypti and aedes albopictus spp. of mosquitoes: a study in khyber pakhtunkhwa, pakistan.dengue is a vector-borne disease caused by dengue virus. according to the recent report of cdc that one-third population of the world are at high risk with dengue fever. the prevalence of the dengue hemorrhagic fever was found more in tropical and sub-tropical regions of the world. aedes mosquitoes was reported as the main cause of transmission of dengue virus. so the current study was planned to characterize the virus in aedes mosquitoes collected from different area of pakistan. in current inv ...201830046621
specific human antibody responses to aedes aegypti and aedes polynesiensis saliva: a new epidemiological tool to assess human exposure to disease vectors in the pacific.aedes mosquitoes severely affect the health and wellbeing of human populations by transmitting infectious diseases. in french polynesia, aedes aegypti is the main vector of dengue, chikungunya and zika, and aedes polynesiensis the primary vector of bancroftian filariasis and a secondary vector of arboviruses. tools for assessing the risk of disease transmission or for measuring the efficacy of vector control programmes are scarce. a promising approach to quantify the human-vector contact relies ...201830040826
is autumn the key for dengue epidemics in non endemic regions? the case of argentina.dengue is a major and rapidly increasing public health problem. in argentina, the southern extreme of its distribution in the americas, epidemic transmission takes place during the warm season. since its re-emergence in 1998 two major outbreaks have occurred, the biggest during 2016. to identify the environmental factors that trigger epidemic events, we analyzed the occurrence and magnitude of dengue outbreaks in time and space at different scales in association with climatic, geographic and dem ...201830038860
the effect of skitosnack, an artificial blood meal replacement, on aedes aegypti life history traits and gut microbiota.public health research and vector control frequently require the rearing of large numbers of vector mosquitoes. all target vector mosquito species are anautogenous, meaning that females require vertebrate blood for egg production. vertebrate blood, however, is costly, with a short shelf life. to overcome these constraints, we have developed skitosnack, an artificial blood meal replacement for the mosquito aedes aegypti, the vector of dengue, zika and chikungunya virus. skitosnack contains bovine ...201830038361
classification of containers with aedes aegypti pupae using a neural networks model.this paper discusses the presence of aedes aegypti pupae in different types of containers considering: volume, ph of the container, among other variables.201830036370
acute inhalation toxicity study of n-heneicosane and its combination with diflubenzuron: an attracticide of aedes aegypti.combination of an oviposition pheromone and an insect growth regulator for the control of vectors is an effective approach. there is a need for toxicological evaluation before its introduction. the present study evaluates the acute inhalation toxicity of n-heneicosane and its combination with diflubenzuron in a head-only inhalation exposure chamber made of glass.201830033812
cgti, a novel thermostable kunitz trypsin-inhibitor purified from cassia grandis seeds: purification, characterization and termiticidal activity.cassia grandis trypsin inhibitor (cgti) is a novel plant serine proteinase inhibitor. this study sets out to purify a thermostable inhibitor from the seeds of cassia grandis and to provide biochemical information about a novel peptide belonging to the kunitz family. moreover, toxicity assays against artemia, aedes aegypti larvae-l4 and nasutitermes corniger are evaluated. the purification process was performed using acetone precipitation, trypsin-sepharose-cl4b and superdex-g75. the inhibitor sh ...201830030073
modelling the distribution of the vector aedes aegypti in a central argentine city.aedes aegypti (diptera: culicidae) is an urban mosquito involved in the transmission of numerous viruses, including dengue, chikungunya and zika. in argentina, ae. aegypti is the main vector of dengue virus and has been involved in several outbreaks in regions ranging from northern to central argentina since 2009. in order to evaluate areas of potential vector-borne disease transmission in the city of córdoba, argentina, the present study aimed to identify the environmental, socioeconomic and de ...201830027565
assessment of insecticide resistance in primary dengue vector, aedes aegypti (linn.) from northern districts of west bengal, india.aedes mosquitoes are the major vectors transmitting several arboviral diseases such as dengue, zika and chikungunya worldwide. northern districts of west bengal is home to several epidemics vectored by mosquito including dengue infections, proper control of which depends on efficient vector control. however the onset of insecticide resistance has resulted in failure of vector control approaches. this study was carried out to unveil the level of insecticide resistance prevailing among the primary ...201830026024
copy number variation (cnv) and insecticide resistance in mosquitoes: evolving knowledge or an evolving problem?copy number variation (cnv) in insect genomes is a rich source of potentially adaptive polymorphism which may help overcome the constraints of purifying selection on conserved genes and/or permit elevated transcription. classic studies of amplified esterases and acetylcholinesterase duplication in culex pipiens quantified evolutionary dynamics of cnv driven by insecticidal selection. a more complex and potentially medically impactful form of cnv is found in anopheles gambiae, with both heterogen ...201830025639
melanization of dirofilaria immitis larvae in different culicid species.dirofilaria immitis is an important filarioid transmitted by culicids. the vector role of these arthropods may be influenced by biological events as melanization against d. immitis larvae. this study aimed to detect the occurrence of melanization in culex quinquefasciatus, aedes (stegomyia) aegypti and aedes (stegomyia) albopictus experimentally infected with d. immitis.201830018997
the use of insecticide-treated curtains for control of aedes aegypti and dengue virus transmission in "fraccionamiento" style houses in méxico.dengue, chikungunya, yellow fever, and zika viruses transmitted by aedes aegypti mosquitoes are major public health threats in the tropical and subtropical world. in méxico, construction of large tracts of "fraccionamientos" high density housing to accommodate population growth and urbanization has provided fertile ground for ae. aegypti-transmitted viruses. we investigated the utility of pyrethroid-treated window curtains to reduce both the abundance of ae. aegypti and to prevent dengue virus ( ...201830018645
zika virus outbreak in the pacific: vector competence of regional vectors.in 2013, zika virus (zikv) emerged in french polynesia and spread through the pacific region between 2013 and 2017. several potential aedes mosquitoes may have contributed to the zikv transmission including aedes aegypti, the main arbovirus vector in the region, and aedes polynesiensis, vector of lymphatic filariasis and secondary vector of dengue virus. the aim of this study was to analyze the ability of these two pacific vectors to transmit zikv at a regional scale, through the evaluation and ...201830016372
molecular characterization of the cry gene profile of bacillus thuringiensis isolated from a caribbean region of colombia.in order to characterize native strains of bacillus thuringiensis of the colombian caribbean with toxic effect against insect vectors, 28 samples of bacteria identified as b. thuringiensis were isolated from different soils and muds around the city of valledupar. using a biological test, five isolates of b. thuringiensis showed toxic effect against larvae of aedes aegypti. pcr methods were used to detect cry1, cry2, cry4b, cry10 and cyt1 genes. cry1 and cry2 genes were detected in 35.7% and 32.1 ...201830015421
evidence of vertical transmission of zika virus in field-collected eggs of aedes aegypti in the brazilian amazon.arboviruses are viruses transmitted to humans and other animals by the bite of hematophagous arthropods. infections caused by chikungunya virus (chikv), dengue virus (denv), zika virus (zikv), and the deadlier yellow fever virus (yfv) are current public health problems in several countries, mainly those located in tropical and subtropical regions. one of the main prevention strategies continues to be vector control, with the elimination of breeding sites and surveillance of infested areas. the u ...201830011278
transmission potential of mayaro virus in florida aedes aegypti and aedes albopictus mosquitoes.mayaro virus (mayv) is an emerging mosquito-borne arbovirus present in central and south america that causes arthralgia and febrile illness. domestic mosquitoes aedes aegypti (diptera: culicidae) and aedes albopictus are potential vectors of mayv that may allow for transmission to humans in urban settings. the present paper assesses susceptibility to infection, disseminated infection and transmission potential in florida ae. aegypti and ae. albopictus for mayv. oral infection was significantly h ...201830006976
the occurrence, diversity and blood feeding patterns of potential vectors of dengue and yellow fever in kacheliba, west pokot county, kenya.yellow fever (yf) and dengue (den) viruses are important re-emerging mosquito-borne viruses sharing similar vectors and reservoirs. the last documented yf outbreak in kenya occurred in 1992-95. however, yf virus is re-emerging in bordering countries including uganda, ethiopia and south sudan with the potential for spread to the neighboring regions in kenya. dengue is endemic in kenya with outbreaks being detected in various towns in the north and the coast. this study reports on the aedes (stego ...201830006028
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