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asymmetrical competition between aedes aegypti and culex quinquefasciatus (diptera: culicidae) coexisting in breeding sites.aedes aegypti and culex quinquefasciatus are mosquito vectors for several tropical diseases that represent a current public health problem. the ecological requirements for each species are different, however, both species show high biological adaptability, which promotes their coexistence in the same breeding sites. the purpose of this study was to assess the effect of larval association between ae. aegypti and cx. quinquefasciatus under different laboratory conditions of food supply and tempera ...201729064390
voltage-sensitive sodium channel mutations s989p + v1016g in aedes aegypti confer variable resistance to pyrethroids, ddt and oxadiazines.aedes aegypti is a vector of several important human pathogens. control efforts rely primarily on pyrethroid insecticides for adult mosquito control, especially during disease outbreaks. a. aegypti has developed resistance nearly everywhere it occurs and insecticides are used. an important mechanism of resistance is due to mutations in the voltage-sensitive sodium channel (vssc) gene. two mutations, in particular, s989p + v1016g, commonly occur together in parts of asia.201729064635
culex quinquefasciatus mosquitoes do not support replication of zika virus.the rapid spread of zika virus (zikv) in the americas raised many questions about the role of culex quinquefasciatus mosquitoes in transmission, in addition to the key role played by the vector aedes aegypti. here we analysed the competence of cx. quinquefasciatus (with or without wolbachia endosymbionts) for a zikv isolate. we also examined the induction of rna interference pathways after viral challenge and the production of small virus-derived rnas. we did not observe any infection nor such s ...201729076805
assessing the population at risk of zika virus in asia - is the emergency really over?on november 18, 2016, the who ended its designation of the zika virus (zikv) epidemic as a public health emergency of international concern (pheic). at the same time, zikv transmission continues in asia, with the number of asian countries reporting zika cases increasing over the last 2 years. applying a method that combines epidemiological theory with data on epidemic size and drivers of transmission, we characterised the population at risk of zikv infection from aedes aegypti mosquitoes in 15 c ...201729082009
darker eggs of mosquitoes resist more to dry conditions: melanin enhances serosal cuticle contribution in egg resistance to desiccation in aedes, anopheles and culex vectors.mosquito vectors lay their white eggs in the aquatic milieu. during early embryogenesis water passes freely through the transparent eggshell, which at this moment is composed of exochorion and endochorion. within two hours the endochorion darkens via melanization but even so eggs shrink and perish if removed from moisture. however, during mid-embryogenesis, cells of the extraembryonic serosa secrete the serosal cuticle, localized right below the endochorion, becoming the third and innermost eggs ...201729084225
functional analysis of glycosylation of zika virus envelope protein.zika virus (zikv) infection causes devastating congenital abnormities and guillain-barré syndrome. the zikv envelope (e) protein is responsible for viral entry and represents a major determinant for viral pathogenesis. like other flaviviruses, the zikv e protein is glycosylated at amino acid n154. to study the function of e glycosylation, we generated a recombinant n154q zikv that lacks the e glycosylation and analyzed the mutant virus in mammalian and mosquito hosts. in mouse models, the mutant ...201729091758
gold nanoparticles - against parasites and insect vectors.nanomaterials are currently considered for many biological, biomedical and environmental purposes, due to their outstanding physical and chemical properties. the synthesis of gold nanoparticles (au nps) is of high interest for research in parasitology and entomology, since these nanomaterials showed promising applications, ranging from detection techniques to drug development, against a rather wide range of parasites of public health relevance, as well as on insect vectors. here, i reviewed curr ...201829092797
detection and serotyping of dengue viruses in aedes aegypti and aedes albopictus (diptera: culicidae) collected in surabaya, indonesia from 2008 to 2015.aedes aegypti and aedes albopictus are the primary and secondary vectors, respectively, of dengue, the most important arboviral disease in the world. the aim of this study was to detect and serotype dengue viruses (denv) in the vectors ae. aegypti and ae. albopictus in surabaya, indonesia. between 2008 and 2015, 16,605 aedes mosquitoes were collected in 15 sub-districts of surabaya. ae. aegypti was dominant (90.9%), whereas few ae. albopictus were collected (9.1%). a total of 330 pools of adult ...201829093315
efficiency of two larval diets for mass-rearing of the mosquito aedes aegypti.aedes aegypti is a major vector of arboviruses that may be controlled on an area-wide basis using the sterile insect technique (sit). larval diet is a major factor in mass-rearing for sit programs. we compared dietary effects on immature development and adult fitness-related characteristics for an international atomic energy agency (iaea) diet, developed for rearing ae. albopictus, and a standardized laboratory rodent diet (lrd), under a 14:10 h (light:dark) photoperiod ("light" treatment) or co ...201729095933
using phage display technology to obtain crybodies active against non-target insects.the insecticidal cry toxins produced by bacillus thuringiensis (bt) are increasingly important in the biological control of insect pests and vectors of human disease. markets for bt products and transgenic plants expressing their toxins are driven by their specificity, safety and the move away from chemical control agents. however, the high specificity of cry toxins can also prove to be a limitation when there is no known cry toxin active against a particular target. novel activities can be disc ...201729097681
the bacterium wolbachia exploits host innate immunity to establish a symbiotic relationship with the dengue vector mosquito aedes aegypti.a host's immune system plays a central role in shaping the composition of the microbiota and, in return, resident microbes influence immune responses. symbiotic associations of the maternally transmitted bacterium wolbachia occur with a wide range of arthropods. it is, however, absent from the dengue and zika vector mosquito aedes aegypti in nature. when wolbachia is artificially forced to form symbiosis with this new mosquito host, it boosts the basal immune response and enhances the mosquito's ...201829099491
functional characterization of the dual allatostatin-a receptors in mosquitoes.the neuropeptide allatostatin-a (asta) and its cognate receptors (astars) are involved in the modulation of feeding behavior, which in hematophagous insects includes the regulation of the disease vector-related behaviors, host seeking and blood feeding. in mosquitoes and other dipterans, there are two copies of astar, contrasting with the single copy found in other insects. in this study, we identified and cloned the dual astar system of two important disease vectors aedes aegypti and culex quin ...201829103918
surveillance of intensity level and geographical spreading of dengue outbreak among males and females in punjab, pakistan: a case study of 2011.dengue fever is viral disease which spreads due to the bite of the aedes aegypti mosquito. in recent years, it has affected around 40% population of the world. its endemic flow has led to a large disease burden, in terms of human and financial resources.201729103928
aedes aegypti molecular responses to zika virus: modulation of infection by the toll and jak/stat immune pathways and virus host factors.zika (zikv) and dengue virus (denv) are transmitted to humans by aedes mosquitoes. however, the molecular interactions between the vector and zikv remain largely unexplored. in this work, we further investigated the tropism of zikv in two different aedes aegypti strains and show that the virus infection kinetics, tissue migration, and susceptibility to infection differ between mosquito strains. we also compare the vector transcriptome changes upon zikv or denv infection demonstrating that 40% of ...201729109710
a blood meal enhances innexin mrna expression in the midgut, malpighian tubules, and ovaries of the yellow fever mosquito aedes aegypti.mosquitoes are vectors of pathogens that cause diseases of medical and veterinary importance. female mosquitoes transmit these pathogens while taking a blood meal, which most species require to produce eggs. the period after a blood meal is a time of extreme physiological change that requires rapid coordination of specific tissues. gap junctions (gj) are intercellular channels that aid in the coordination of cells within tissues via the direct transfer of certain small molecules and ions between ...201729113099
assessment of achyranthes aspera induced toxicity and molecular analysis of rapd-pcr profiles of larval genomic dna of aedes aegypti l. (diptera: culicidae).current studies investigated the anti-mosquito potential of achyranthes aspera against the dengue vector, aedes aegypti. the stems and leaves of a. aspera were extracted in hexane and evaluated for their toxicity against early fourth instars of a. aegypti. the larvicidal efficacy of the extract was validated as per who protocol. the mortality counts were made after 24 h and lc values were calculated at different levels. the adverse impact of extracts was also explored on the larval genomic dna. ...201729114143
the impact of temperature and wolbachia infection on vector competence of potential dengue vectors aedes aegypti and aedes albopictus in the transmission of dengue virus serotype 1 in southern taiwan.we evaluated the impact of temperature and wolbachia infection on vector competence of the local aedes aegypti and ae. albopictus populations of southern taiwan in the laboratory.201729116011
environmentally benign nanometric neem-laced urea emulsion for controlling mosquito population in environment.the increasing risk of vector-borne diseases and the environmental pollution in the day-to-day life due to the usage of the conventional pesticides makes the role of nanotechnology to come into the action. the current study deals with one of the applications of nanotechnology through the formulation of neem urea nanoemulsion (nune). nune was formulated using neem oil, tween 20, and urea using the microfluidization method. prior to the development of nanoemulsion, the ratio of oil/surfactant/urea ...201829116538
incidence of dengue and chikungunya viruses in mosquitoes and human patients in border provinces of vietnam.dengue virus remains a major threat in vietnam, while chikungunya virus is expected to become one. surveillance was conducted from 2012 to 2014 in vietnam to assess the presence of dengue and chikungunya viruses in patients hospitalized with acute fever in five vietnam provinces neighboring lao pdr and cambodia. surveillance was extended to mosquitoes present in the vicinity of the patients' households.201729121985
whatch'em: a weather-driven energy balance model for determining water height and temperature in container habitats for aedes aegypti.the mosquito virus vector aedes (ae.) aegypti exploits a wide range of containers as sites for egg laying and development of the immature life stages, yet the approaches for modeling meteorologically sensitive container water dynamics have been limited. this study introduces the water height and temperature in container habitats energy model (whatch'em), a state-of-the-science, physically based energy balance model of water height and temperature in containers that may serve as development sites ...201629123363
first record of aedes aegypti and aedes albopictus in thirteen panhandle region counties of texas, u.s.a. 201729125249
ecological characterization of aedes aegypti larval habitats (diptera: culicidae) in artificial water containers in girardot, colombia.the establishment of habitats for immature ae. aegypti is regulated by biotic and abiotic factors and interactions between these factors. this study aimed to determine the effects of physico-chemical variables and planktonic algae on immature ae. aegypti habitats in 101 water tanks (50 of them containing ae. aegypti pupae and/or larvae) in girardot, colombia. physical data were collected from the water tanks (volume, capacity, material, detritus, and location), along with the physico-chemical va ...201729125250
the distribution of mosquitoes across an altitudinal gradient in the galapagos islands.an avian malaria parasite (genus plasmodium) has been detected consistently in the galapagos penguin (spheniscus mendiculus) and less frequently in some passerines. we sampled three resident mosquito species (aedes taeniorhynchus, culex quinquefasciatus, and aedes aegypti) using cdc light and gravid traps on three islands in 2012, 2013, and 2014. we sampled along altitudinal gradients to ask whether there are mosquito-free refugia at higher elevations as there are in hawaii. we captured both ae. ...201729125252
evaluation of sticky traps for adult aedes mosquitoes in malaysia: a potential monitoring and surveillance tool for the efficacy of control strategies.the present study compared the performance of sticky traps in order to identify the most effective and practical trap for capturing aedes aegypti and aedes albopictus mosquitoes. three phases were conducted in the study, with phase 1 evaluating the five prototypes (models a, b, c, d, and e) of sticky trap release-and-recapture using two groups of mosquito release numbers (five and 50) that were released in each replicate. similarly, phase 2 compared the performance between model e and the classi ...201729125255
infection of a french population of aedes albopictus and of aedes aegypti (paea strain) with zika virus reveals low transmission rates to these vectors' saliva.disease caused by the zika virus (zikv) is a public health emergency of international concern. recent epidemics have emerged in different regions of the world and attest to the ability of the virus to spread wherever its vector, aedes species mosquitoes, can be found. we have compared the transmission of zikv by ae. aegypti (paea strain originating from tahiti) and by a french population of ae. albopictus to better assess their competence and the potential risk of the emergence of zikv in europe ...201729125545
biosurfactants produced by scheffersomyces stipitis cultured in sugarcane bagasse hydrolysate as new green larvicides for the control of aedes aegypti, a vector of neglected tropical diseases.biosurfactants are microbial metabolites with possible applications in various industrial sectors that are considered ecofriendly molecules. in recent years, some studies identified these compounds as alternatives for the elimination of vectors of tropical diseases, such as aedes aegypti. the major bottlenecks of biosurfactant industrial production have been the use of conventional raw materials that increase production costs as well as opportunistic or pathogenic bacteria, which restrict the ap ...201729125845
the diversity, structure, and function of heritable adaptive immunity sequences in the aedes aegypti genome.the aedes aegypti mosquito transmits arboviruses, including dengue, chikungunya, and zika virus. understanding the mechanisms underlying mosquito immunity could provide new tools to control arbovirus spread. insects exploit two different rnai pathways to combat viral and transposon infection: short interfering rnas (sirnas) and piwi-interacting rnas (pirnas) [1, 2]. endogenous viral elements (eves) are sequences from non-retroviral viruses that are inserted into the mosquito genome and can act a ...201729129531
behavior of detoxifying enzymes of aedes aegypti exposed to girgensohnine alkaloid analog and cymbopogon flexuosus essential oil.because mosquito control depend on the use of commercial insecticides and resistance has been described in some of them, there is a need to explore new molecules no resistant. in vivo effects of girgensohnine analog 2-(3,4-dimethoxyphenyl)-2-(piperidin-1-yl)acetonitrile dppa and cymbopogon flexuosus essential oil cfeo, on the detoxifying enzymes acetylcholinesterase (ache), glutathione-s-transferase (gst), nonspecific esterases (α- and β-), mixed function oxidases (mfo) and p-npa esterases were ...201829129589
zika virus: what we need to know?zika virus is one of the emerging viruses and is of significant threat to human health globally. it is a mosquito borne flavivirus similar to dengue, yellow fever, and west nile viruses. it was reported about 5 decades ago and then it spreads to different parts of the world. large outbreaks were reported on yap islands in 2007. now it has gained wide attention globally by health communities. major vector for virus transmission is aedes aegypti mosquito. zikv infection is mostly asymptomatic but ...201829131357
functional characterization of a serine protease inhibitor modulated in the infection of the aedes aegypti with dengue virus.during feeding with blood meal, female aedes aegypti can transmit infectious agents, such as dengue, yellow fever, chikungunya and zika viruses. dengue virus causes human mortality in tropical regions of the world, and there is no specific treatment or vaccine with maximum efficiency being used for these infections. in the vector-virus interaction, the production of several molecules is modulated by both mosquitoes and invading agents. however, little information is available about these molecul ...201829133118
germline cas9 expression yields highly efficient genome engineering in a major worldwide disease vector, aedes aegypti.the development of crispr/cas9 technologies has dramatically increased the accessibility and efficiency of genome editing in many organisms. in general, in vivo germline expression of cas9 results in substantially higher activity than embryonic injection. however, no transgenic lines expressing cas9 have been developed for the major mosquito disease vector aedes aegypti here, we describe the generation of multiple stable, transgenic ae. aegypti strains expressing cas9 in the germline, resulting ...201729138316
designing ecofriendly bionanocomposite assembly with improved antimicrobial and potent on-site zika virus vector larvicidal activities with its mode of action.dialyzed natural polymer, fibroin, from bombyx mori was used to synthesize biocompatible silver and gold nanoparticles in-situ in dispersion form. the films of pure fibroin (pf), fibroin-silver nanocomposite (fsnc) and fibroin-gold nanocomposite (fgnc) were fabricated by drop casting method. the characterization of the resultant dispersion and films was performed by visual color change, uv-vis spectroscopy and atomic force microscopy. the dispersions of pf, fsnc and fgnc were tested for antibact ...201729138496
potential role for flubendazole in limiting filariasis transmission: observations of microfilarial sensitivity.flubendazole (flbz) is a potent and efficacious macrofilaricide after parenteral administration. studies in animal models and one trial in patients infected with onchocerca volvulus revealed that flbz elicits minimal effects on microfilariae (mf). severe complications after ivermectin (ivm) treatment of patients with high loa loa microfilaraemia are of great concern. we examined the potential of flbz to rapidly kill l. loa mf, the phenomenon proposed to underlie the complications. mf of l. loa w ...201829141764
molecular detection and characterization of wolbachia pipientis from culex quinquefasciatus collected from lahore, pakistan.the gram-negative, pleomorphic endosymbiont wolbachia is known to infect a large number of insects and other arthropods naturally. this bacterium modifies the host biology, mainly causing reproductive alterations including feminization, death of male, parthenogenesis, and importantly cytoplasmic incompatibility. wolbachia-induced cytoplasmic incompatibility results in nonviable offspring and vector population suppression. in addition, this bacterium rapidly spreads and propagates within the host ...201829141766
corrigendum: rna-seq comparison of larval and adult malpighian tubules of the yellow fever mosquito aedes aegypti reveals life stage-specific changes in renal function.[this corrects the article on p. 283 in vol. 8, pmid: 28536536.].201729142533
blocking the transmission of heartworm (dirofilaria immitis) to mosquitoes (aedes aegypti) by weekly exposure for one month to microfilaremic dogs treated once topically with dinotefuran-permethrin-pyriproxyfen.this study assessed the influence of a topical ectoparasiticide (dinotefuran-permethrin-pyriproxyfen, dpp, vectra®3d, ceva animal health) on the acquisition of heartworm microfilariae by mosquitoes exposed to microfilaremic dogs weekly for 1 month.201729143651
shifting the paradigm in dirofilaria immitis prevention: blocking transmission from mosquitoes to dogs using repellents/insecticides and macrocyclic lactone prevention as part of a multimodal approach.this study assessed the influence of a topical ectoparasiticide (dinotefuran-permethrin-pyriproxyfen, dpp, vectra® 3d, ceva animal health) combined with a macrocyclic lactone (milbemycin oxime, mbo, interceptor®, virbac) on transmission of heartworm l3 from mosquitoes to dogs and subsequent development of worms in treated dogs exposed to infected mosquitoes.201729143678
zika virus: an updated review of competent or naturally infected mosquitoes.zika virus (zikv) is an arthropod-borne virus (arbovirus) that recently caused outbreaks in the americas. over the past 60 years, this virus has been observed circulating among african, asian, and pacific island populations, but little attention has been paid by the scientific community until the discovery that large-scale urban zikv outbreaks were associated with neurological complications such as microcephaly and several other neurological malformations in fetuses and newborns. this paper is a ...201729145400
behavioral, climatic, and environmental risk factors for zika and chikungunya virus infections in rio de janeiro, brazil, 2015-16.the burden of arboviruses in the americas is high and may result in long-term sequelae with infants disabled by zika virus infection (zikv) and arthritis caused by infection with chikungunya virus (chikv). we aimed to identify environmental drivers of arbovirus epidemics to predict where the next epidemics will occur and prioritize municipalities for vector control and eventual vaccination. we screened sera and urine samples (n = 10,459) from residents of 48 municipalities in the state of rio de ...201729145452
development and physiological effects of an artificial diet for wolbachia-infected aedes aegypti.the endosymbiotic bacterium wolbachia spreads rapidly through populations of aedes aegypti mosquitoes, and strongly inhibits infection with key human pathogens including the dengue and zika viruses. mosquito control programs aimed at limiting transmission of these viruses are ongoing in multiple countries, yet there is a dearth of mass rearing infrastructure specific to wolbachia-infected mosquitoes. one example is the lack of a blood meal substitute, which accounts for the wolbachia-specific ph ...201729146940
[dengue fever : symptoms, epidemiology, entomology, pathogen diagnosis and prevention].dengue infections are among the most frequent causes of febrile disease in tropical climates. infections are caused by a flavivirus transmitted by the aedes mosquito. aedes aegypti mosquitos are the main transmitters of dengue viruses. since these insects are both diurnal and container breeders, particular prevention and control measures are required. symptom severity varies and can range from a mild, flu-like clinical picture to severe hemorrhage and shock. the most common symptoms experienced ...201729147722
baccharis reticularia dc. and limonene nanoemulsions: promising larvicidal agents for aedes aegypti (diptera: culicidae) control.baccharis reticularia dc. is a plant species from the asteraceae family that is endemic to brazil. despite the great importance of baccharis genus, no study has been carried out regarding either the phytochemical composition of b. reticularia or the evaluation of its larvicidal potential. considering the intrinsic immiscibility of essential oils, this study shows larvicidal nanoemulsions containing the b. reticularia phytochemically characterized essential oil and its main constituent against ae ...201729149027
the introduction of dengue follows transportation infrastructure changes in the state of acre, brazil: a network-based analysis.human mobility, presence and passive transportation of aedes aegypti mosquito, and environmental characteristics are a group of factors which contribute to the success of dengue spread and establishment. to understand this process, we assess data from dengue national and municipal basins regarding population and demographics, transportation network, human mobility, and ae. aegypti monitoring for the brazilian state of acre since the first recorded dengue case in the year 2000 to the year 2015. d ...201729149175
vectors, hosts, and control measures for zika virus in the americas.we examine zika virus (zikv) from an ecological perspective and with a focus on the americas. we assess (1) the role of wildlife in zikv disease ecology, (2) how mosquito behavior and biology influence disease dynamics, and (3) how nontarget species and ecosystems may be impacted by vector control programs. our review suggests that free-ranging, non-human primates may be involved in zikv transmission in the old world; however, other wildlife species likely play a limited role in maintaining or t ...201729150828
effective population sizes of a major vector of human diseases, aedes aegypti.the effective population size (ne ) is a fundamental parameter in population genetics that determines the relative strength of selection and random genetic drift, the effect of migration, levels of inbreeding, and linkage disequilibrium. in many cases where it has been estimated in animals, ne is on the order of 10%-20% of the census size. in this study, we use 12 microsatellite markers and 14,888 single nucleotide polymorphisms (snps) to empirically estimate ne in aedes aegypti, the major vecto ...201729151858
a call to introduce structured zika surveillance in india.india has the climatic conditions conducive to year-round transmission of zika virus, and a structured disease surveillance program should be implemented to prevent an outbreak. such a program should (i) start screening before an outbreak arises; (ii) collect baseline data to assess future disease risk and monitor potential birth defects; and (iii) provide new insights into the ecology of the disease and inform public health policy following the one health concept.201729153262
upregulation of aedes aegypti vago1 by wolbachia and its effect on dengue virus replication.dengue infection along with its related disease conditions poses a significant threat to human health. the pathogen responsible for this infection is dengue virus (denv) which is primarily transmitted to humans through the bites of aedes aegypti mosquitoes. unavailability of a potent vaccine has recently sparked renewed research endeavours aimed at vector control. to date, wolbachia as an endosymbiotic bacterium has shown promise as a novel biocontrol agent to restrict denv replication in the ve ...201729157676
alternative control of aedes aegypti resistant to pyrethroids: lethal and sublethal effects of monoterpenes bioinsecticides.the mosquito aedes aegypti is intensely controlled because it is vector of viruses that cause innumerous diseases, especially in tropical regions. due to the indiscriminate use of insecticides, populations from different regions have been resistant to pyrethroids. here, we analyzed the lethal and sublethal effects of essential oil of aristolochia trilobata and its major compounds on a. aegypti from susceptible and pyrethroid resistant populations.201729160036
[spatial distribution of aedes aegypti (diptera: culicidae) in the rural area of two municipalities of cundinamarca, colombia].it is difficult to adapt or create new strategies for dengue control in rural areas due to the dispersion of homes and the lack of knowledge about the presence and behavior of the vector in these areas. spatial data analysis could help understand time and space dynamics related to the transmission of vector-borne diseases in rural areas and, thus, contribute to the prevention and control of dengue.201729161476
[spatial distribution of potential and positive aedes aegypti breeding sites].the spatial distribution of aedes aegypti is heterogeneous, and the interaction between positive and potential breeding sites located both inside and outside homes is one of the most difficult aspects to characterize in vector control programs.201729161478
[laboratory susceptibility tests of aedes aegypti and culex quinquefasciatus larvae to the entomopathogenic nematode heterorhabditis bacteriophora].aedes aegypti is the vector of dengue, yellow fever, zika and chikungunya viruses, and culex quinquefasciatus is the vector of st. louis and west nile encephalitis viruses.201729161479
aedes albopictus (skuse, 1894) infected with the american-asian genotype of dengue type 2 virus in medellín suggests its possible role as vector of dengue fever in colombia.aedes aegypti and ae. albopictus are recognized vectors of dengue, yellow fever, chikungunya and zika arboviruses in several countries worldwide. in colombia, ae. albopictus geographical distribution has increased to include highly populated cities such as cali and medellín. although this species has been frequently found in urban and semi-urban zones in the country, its role as vector of the dengue fever is poorly known.201729161485
virological surveillance of aedes (stegomyia) aegypti and aedes (stegomyia) albopictus as support for decision making for dengue control in medellín.dengue represents an important public health problem in colombia. no treatment is available and the vaccine has not been approved in all countries, hence, actions should be strengthened to mitigate its impact through the control of aedes aegypti, the vector mosquito. in colombia, surveillance is done using entomological indexes and case notification, which is usually informed late, leading to untimely interventions. viral detection in urban mosquitoes using molecular techniques provides more acc ...201729161487
effect of the type and number of washes in the efficacy of long lasting insecticidal nets for aedes aegypti control in girardot, colombia.the use of long lasting insecticidal materials has shown promising results in the control of aedes aegypti.201729161489
dengue virus detection in aedes aegypti larvae and pupae collected in rural areas of anapoima, cundinamarca, colombia.there is a high incidence and prevalence of dengue in the department of cundinamarca, and recently aedes aegypti, the main vector of dengue virus (denv), was detected in some of its rural areas.201729161491
emergent acoustic order in arrays of mosquitoes.the yellow fever mosquito aedes aegypti forms aerial swarms that serve as mating aggregations [1]. despite lacking the remarkable collective order of other animal ensembles, such as fish and birds [2], the kinematic properties of these swarms bear the hallmarks of local interaction and global cohesion [3,4]. however, the mechanisms responsible for collective behaviour in mosquitoes are not well understood. mosquitoes use their antennae as hearing organs to locate and interact with one another vi ...201729161555
complex wolbachia infection dynamics in mosquitoes with imperfect maternal transmission.dengue, malaria, and zika are dangerous diseases primarily transmitted by aedes aegypti, aedes albopictus, and anopheles stephensi. in the last few years, a new disease control method, besides pesticide spraying to kill mosquitoes, has been developed by releasing mosquitoes carrying bacterium wolbachia into the natural areas to infect the wild population of mosquitoes and block disease transmission. the bacterium is transmitted by infected mothers and the maternal transmission was assumed to be ...201829161848
larvicidal, histopathological efficacy of penicillium daleae against larvae of culex quinquefasciatus and aedes aegypti plus biotoxicity on artemia nauplii a non-target aquatic organism.mosquitoes can transmit the terrible diseases to human beings. soil-borne fungal products act as potential source for low-cost chemicals, used for developing eco-friendly control agents against mosquito-vector borne diseases. the prime aim of study was to check the larvicidal potential of fungus mycelia (by ethyl acetate solvent) extract from penicillium daleae (kx387370) against culex quinquefasciatus and aedes aegypti and to test the toxicity of brine shrimp artemia nauplii, by observing the p ...201729163159
insecticide susceptibility status in mexican populations of stegomyia aegypti (= aedes aegypti): a nationwide assessment.in mexico, mosquito vector-borne diseases are of public health concern as a result of their impact on human morbidity and mortality. the use of insecticides against adult mosquitoes is one of the most common ways of controlling mosquito population densities. however, the use of these compounds has resulted in the development of insecticide resistance. the aim of this study was to estimate susceptibility to six pyrethroids, two carbamates and two organophosphates in mexican populations of stegomy ...201729165810
wolbachia infection alters the relative abundance of resident bacteria in adult aedes aegypti mosquitoes, but not larvae.insect-symbiont interactions are known to play key roles in host functions and fitness. the common insect endosymbiont wolbachia can reduce the ability of several human pathogens, including arboviruses and the malaria parasite, to replicate in insect hosts. wolbachia does not naturally infect aedes aegypti, the primary vector of dengue virus, but transinfected ae. aegypti have antidengue virus properties and are currently being trialled as a dengue biocontrol strategy. here, we assess the impact ...201729165845
feeding-induced changes in allatostatin-a and short neuropeptide f in the antennal lobes affect odor-mediated host seeking in the yellow fever mosquito, aedes aegypti.aedes aegypti is a model species in which the endogenous regulation of odor-mediated host seeking behavior has received some attention. sugar feeding and host seeking in female a. aegypti are transiently inhibited following a blood meal. this inhibition is partially mediated by short neuropeptide f (snpf). the paired antennal lobes (als), as the first processing centers for olfactory information, has been shown to play a significant role in the neuropeptidergic regulation of odor-mediated behavi ...201729166650
biology of two larval morphological phenotypes of aedes aegypti in abidjan, côte d'ivoire.since 2008, several outbreaks of yellow fever and dengue occurred in abidjan, the economic capital of côte d'ivoire. a better knowledge of the biology of aedes aegypti populations, the main vector of yellow fever and dengue viruses, is necessary to tailor vector control strategies implemented in the city. this study was designed to determine some biological parameters, occurring during the life cycle of two morphological phenotypes of ae. aegypti larvae. mosquitoes were sampled in a suburb of ab ...201729166962
determination of the efficiency of diets for larval development in mass rearing aedes aegypti (diptera: culicidae).larval diet quality and rearing conditions have a direct and irreversible effect on adult traits. therefore, the current study was carried out to optimize the larval diet for mass rearing of aedes aegypti, for sterile insect technique (sit)-based applications in sri lanka. five batches of 750 first instar larvae (l 1) of ae. aegypti were exposed to five different concentrations (2-10%) of international atomic energy agency (iaea) recommended the larval diet. morphological development parameters ...201729166980
the importance of human population characteristics in modeling aedes aegypti distributions and assessing risk of mosquito-borne infectious diseases.the mosquito aedes aegypti has long been a vector for human illness in the southeastern united states. in the past, it has been responsible for outbreaks of dengue, chikungunya, and yellow fever and, very recently, the zika virus that has been introduced to the region. multiple studies have modeled the geographic distribution of ae. aegypti as a function of climate factors; however, this ignores the importance of humans to the anthropophilic biter. furthermore, ae. aegypti thrives in areas where ...201729167627
facile green synthesis of zinc oxide nanoparticles using ulva lactuca seaweed extract and evaluation of their photocatalytic, antibiofilm and insecticidal activity.the bioactivity of semiconductor nanocomplexes has been poorly studied in the field of pesticide science. in this research, the synthesis of zinc nanoparticles was accomplished through new effortless green chemistry process, using the ulva lactuca seaweed extract as a reducing and capping agent. the production of u. lactuca-fabricated zno nanoparticles (ul-zno nps) was characterized by powder x-ray diffraction (xrd), uv-visible, fourier transform infrared (ftir) spectroscopy, selected area elect ...201829169140
bacterial exopolysaccharide (eps)-coated zno nanoparticles showed high antibiofilm activity and larvicidal toxicity against malaria and zika virus vectors.in this study, a novel and effective approach was performed to synthesize zno nanoparticles (zno nps) using the exopolysaccharides (eps) from the probiotic strain bacillus licheniformis dahb1. eps acted as reducing and stabilizing agent for the formation of eps-zno nps by co-precipitation method. structural characterization was investigated by a surface plasma resonance centered at 375nm in uv-vis spectrum. ftir spectrum exhibited functional groups with strong absorption peak at 3814.7-420cm-1. ...201829173489
a peptide signaling system that rapidly enforces paternity in the aedes aegypti mosquito.female aedes aegypti mosquitoes typically mate only once with one male in their lifetime, a behavior known as "monandry" [1]. this single mating event provisions the female with sufficient sperm to fertilize the >500 eggs she will produce during her ∼4- to 6-week lifespan in the laboratory [2]. successful mating induces lifetime refractoriness to subsequent insemination by other males, enforcing the paternity of the first male [3-5]. ae. aegypti mate in flight near human hosts [6], and females b ...201729174895
involvement of cecropin b in the formation of the aedes aegypti mosquito cuticle.in this study, we found a mosquito antimicrobial peptide (amp), aedes aegypti cecropin b (aacec b), was expressed constitutively in pupae. knockdown in the pupae of aacec b using double-stranded rna (dsrna) resulted in high mortality, the emergence of deformed adults and an impairment of pharate adult cuticle formation with fewer lamellae being deposited and the helicoidal pattern of the chitin microfibrils being disorganized. simultaneous injection of aacec b dsrna and aacec b peptide into pupa ...201729180688
point-of-use nanobiosensor for detection of dengue virus ns1 antigen in adult aedes aegypti: a potential tool for improved dengue surveillance.dengue virus (denv) and its primary mosquito vectors aedes spp. have spread to every continent except antarctica, causing outbreaks and autochthonous transmission in previously disease-free regions. recently, the spread of other arboviruses carried by invasive aedes spp., such as chikungunya and zika, seem to be following similar trends as denv and have renewed interest in monitoring and modeling arboviruses within mosquito vectors. unfortunately, current commercially available detection methods ...201829182305
first evidence of zika virus venereal transmission in aedes aegypti mosquitoes.aedes aegypti is considered the main zika virus (zikv) vector, and is thought to be responsible for the 2015-2016 outbreak in brazil. zika positive ae. aegypti males collected in the field suggest that vertical and/or venereal transmission of zikv may occur.201829185595
insecticide susceptibility screening against culex and aedes (diptera: culicidae) mosquitoes from the united states.mosquitoes exposed to sublethal doses of insecticides may be selected for resistance to insecticide active ingredients (ais). mosquitoes are exposed to ais through agricultural, public/private mosquito control programs, homeowners, and other sources. hence, mosquito control programs should routinely measure the resistance/susceptibility status of mosquito populations of public health concern. the objectives here were to determine resistance status for six ais used in adult mosquito control in th ...201729186579
mapping the spatial distribution of aedes aegypti and aedes albopictus.mosquito-borne infectious diseases, such as rift valley fever, dengue, chikungunya and zika, have caused mass human death with the transnational expansion fueled by economic globalization. simulating the distribution of the disease vectors is of great importance in formulating public health planning and disease control strategies. in the present study, we simulated the global distribution of aedes aegypti and aedes albopictus at a 5×5km spatial resolution with high-dimensional multidisciplinary ...201829191515
first insights on insecticidal activity upon aedes aegypti and partial biochemical characterization of a novel low molecular mass chymotrypsin-trypsin inhibitor purified from lonchocarpus sericeus seeds.arboviroses such as dengue, zika and chikungunya represent a serious issue in public health due to the absence of approved vaccine or specific antiviral drugs. one way to prevent these diseases is by combating the vector mosquito, aedes aegypti (diptera), which has serine proteases in the midgut. protease inhibitors are molecules that can block enzyme activity impairing digestion and nutrition that can lead to death. thus, we purified and characterized a novel chymotrypsin- trypsin inhibitor fro ...201729193604
larvicidal and residual activity of imidazolium salts against aedes aegypti (diptera: culicidae).aedes aegypti is an important species of mosquito, which can transmit several arboviruses such as dengue fever, yellow fever, chikungunya, and zika. as these mosquitoes are becoming resistant to most chemical insecticides used in the world, studies with new larvicides should be prioritized. based on the known biological profile of imidazolium salts (is), the objective of this study was to evaluate the potential of six is as larvicides against ae. aegypti, which was tested against ae. aegypti lar ...201729193680
global risk mapping for major diseases transmitted by aedes aegypti and aedes albopictus.the objective of this study was to map the global risk of the major arboviral diseases transmitted by aedes aegypti and aedes albopictus by identifying areas where the diseases are reported, either through active transmission or travel-related outbreaks, as well as areas where the diseases are not currently reported but are nonetheless suitable for the vector.201729196275
global transcriptome analysis of aedes aegypti mosquitoes in response to zika virus infection.zika virus (zikv) of the flaviviridae family is a recently emerged mosquito-borne virus that has been implicated in the surge of the number of microcephaly instances in south america. the recent rapid spread of the virus led to its declaration as a global health emergency by the world health organization. the virus is transmitted mainly by the mosquito aedes aegypti, which is also the vector of dengue virus; however, little is known about the interactions of the virus with the mosquito vector. i ...201729202041
dengue fever outbreaks in eritrea, 2005-2015: a case for strengthening surveillance, control and reporting.the geographic distribution and burden of dengue is increasing globally. this study aims to evaluate dengue outbreaks and to substantiate the need for strengthened surveillance, reporting and control in eritrea.201629202065
alternative patterns of sex chromosome differentiation in aedes aegypti (l).some populations of west african aedes aegypti, the dengue and zika vector, are reproductively incompatible; our earlier study showed that divergence and rearrangements of genes on chromosome 1, which bears the sex locus (m), may be involved. we also previously described a proposed cryptic subspecies senaae (pk10, senegal) that had many more high inter-sex fst genes on chromosome 1 than did ae.aegypti aegypti (aaa, pai lom, thailand). the current work more thoroughly explores the significance of ...201729202694
an aedes aegypti-associated fungus increases susceptibility to dengue virus by modulating gut trypsin activity.transmission of dengue virus (denv) requires successful completion of the infection cycle in the aedes aegypti vector, which is initiated in the midgut tissue after ingestion of an infectious blood meal. while certain ae. aegypti midgut-associated bacteria influence virus infection, little is known about the midgut-associated fungi (mycobiota), and how its members might influence susceptibility to denv infection. we show that a talaromyces (tsp_pr) fungus, isolated from field-caught ae. aegypti, ...201729205153
dengue serotype-specific immune response in aedes aegypti and aedes albopictus.dengue viruses (denv) are considered one of the most important emerging pathogens and dengue disease is a global health threat. the geographic expansion of dengue viruses has led to co-circulation of all four dengue serotypes making it imperative that new denv control strategies be devised.201729211244
novel wolbachia-transinfected aedes aegypti mosquitoes possess diverse fitness and vector competence phenotypes.wolbachia pipientis from drosophila melanogaster (wmel) is an endosymbiotic bacterium that restricts transmission of human pathogenic flaviviruses and alphaviruses, including dengue, zika, and chikungunya viruses, when introduced into the mosquito vector aedes aegypti. to date, wmel-infected ae. aegypti have been released in field trials in 5 countries to evaluate the effectiveness of this strategy for disease control. despite the success in establishing wmel-infected mosquitoes in wild populati ...201729216317
differences in prevalence of symptomatic zika virus infection by age and sex-puerto rico, 2016.during the zika virus (zikv) outbreak in puerto rico in 2016, non-pregnant women aged 20-39 years were disproportionately identified with zikv disease. we used household-based cluster investigations to determine if this disparity was associated with age- or sex-dependent differences in the rate of zikv infection or reporting symptoms.201729216376
the immune strategies of mosquito aedes aegypti against microbial infection.yellow fever mosquito aedes aegypti transmits many devastating arthropod-borne viruses (arboviruses), such as dengue virus, yellow fever virus, chikungunya virus, and zika virus, which cause great concern to human health. mosquito control is an effective method to block the spread of infectious diseases. ae. aegypti uses its innate immune system to fight against arboviruses, parasites, and fungi. in this review, we briefly summarize the recent findings in the immune response of ae. aegypti again ...201729217264
the gastric caecum of larval aedes aegypti: stimulation of epithelial ion transport by 5-hydroxytryptamine and camp.we report measurements of ion transport across the gastric caecum of larvae of aedes aegypti, a vector of yellow fever that inhabits a variety of aquatic habitats ranging from freshwater to brackish water. we provide the first measurements of the effect of 5-hydroxytryptamine (5-ht) on transepithelial potential (tep), luminal ion concentrations and electrochemical potentials, as well as basolateral membrane potential and h+, na+ and k+ fluxes. tep, basolateral membrane potential, and h+, k+, and ...201729217627
full-length genome sequence of a chikungunya virus isolate from the 2017 autochthonous outbreak, lazio region, italy.we report here the genome sequence of a human chikungunya virus isolate from the ongoing autochthonous outbreak in central italy. the sequence (east-central-south african lineage, indian ocean sublineage), which is similar to recent sequences from pakistan and india, shows e1 and e2 signatures of strains whose main mosquito vector is aedes aegypti, although aedes albopictus is the vector in italy.201729217791
the first detected airline introductions of yellow fever mosquitoes (aedes aegypti) to europe, at schiphol international airport, the netherlands.air-borne introduction of exotic mosquitoes to schiphol airport in the netherlands has been considered plausible based upon findings of mosquitoes in aircraft cabins during 2008, 2010 and 2011. beginning in 2013, surveillance efforts at schiphol had focused on promptly detecting accidental introductions at the airport facilities in order to quickly react and avoid temporary proliferation or establishment of mosquito populations, identify the origin of the introductions, and avoid potential trans ...201729221490
species specific rna a-to-i editing of mosquito rdl modulates gaba potency and influences agonistic, potentiating and antagonistic actions of ivermectin.the insect gaba receptor, rdl, is the target of several classes of pesticides. the peptide sequences of rdl are generally highly conserved between diverse insects. however, rna a-to-i editing can effectively alter amino acid residues of rdl in a species specific manner, which can affect the potency of gaba and possibly insecticides. we report here that rna a-to-i editing alters the gene products of rdl in three mosquito disease vectors, recoding five amino acid residues in rdl of aedes aegypti a ...201729223796
in silico validation of d7 salivary protein-derived b- and t-cell epitopes of aedes aegypti as potential vaccine to prevent transmission of flaviviruses and togaviruses to humans.mosquito (aedes aegyptii) salivary proteins play a crucial role in facilitating viral transmission from vector-to-host due to their role in facilitating the "blood meal" of the vector. three main proteins, d7, aegyptin and sialokinin play a role in this process. using in-silico programs, we identified b- and t-cell epitopes in the mosquito salivary proteins d7 long and short form. t-cell epitopes with high affinity to the most prevalent hla mhc class-i supertypes among different population group ...201729225429
efficacy of 13 commercial household aerosol insecticides against aedes aegypti (diptera: culicidae) from morelos, mexico.in mexico, aedes aegypti (l.) (diptera: culicidae) is the primary vector of dengue, zika, and chikungunya viruses. control programs include community participation using personal protection such as household aerosol insecticides. in both, urban or rural areas, the use of aerosol insecticides is a common practice to avoiding mosquito biting. thus, information on the efficacy of commercial products must be available. this study reports the efficacy of 13 household aerosol insecticides against ae. ...201729228197
detection of zika virus in aedes mosquitoes from mexico.we report on the results of an entomovirological surveillance system of aedes populations performed by the ministry of health of the central state of san luis potosí, mexico.201729232453
aedes aegypti breeding site in an underground rainwater reservoir: a warning.we describe the discovery of aedes aegypti underground breeding site in the pinheiros neighborhood of são paulo, sp, during an entomological survey program performed in 2016. even with intense surveillance and vector control, large numbers of mosquitoes were present in this area. a detailed investigation allowed for the detection of ae. aegypti in an underground reservoir used for rainwater storage. after the implementation of protection screens in the accesses, the presence of the vector was no ...201729236877
assessing natural infection with zika virus in the southern house mosquito, culex quinquefasciatus, during 2016 in puerto rico.the epidemic of zika in the western hemisphere has led to intense investigations of all species important in the transmission of zika virus (zikv), including putative mosquito vectors. although evidence points to stegomyia (= aedes) (diptera: culicidae) mosquitoes as the primary vectors in nature among humans, there remains the possibility that other common mosquito species may be implicated in the rapid spread of the virus. herein, field-caught culex quinquefasciatus (diptera: culicidae) collec ...201729239003
analytical report of the 2016 dengue outbreak in córdoba city, argentina.after elimination of the aedes aegypti vector in south america in the 1960s, dengue outbreaks started to reoccur during the 1990s; strongly in argentina since 1998. in 2016, córdoba city had the largest dengue outbreak in its history. in this article we report this outbreak including spatio-temporal analysis of cases and vectors in the city. a total of 653 dengue cases were recorded by the laboratory-based dengue surveillance system and georeferenced by their residential addresses. case maps wer ...201729239555
nanotitania crystals induced efficient photocatalytic color degradation, antimicrobial and larvicidal activity.textile industries release tonnes of harmful toxic dyes into the environment, causing severe effects on living organisms, including humans. mosquitoes vectors spread important diseases which cause millions of human deaths worldwide. to control mosquitoes a number of synthetic mosquitocidal agents have been employed but all these pesticides pose harmful effects to human health and non-target species and also led to resistance development in treated vectors. microbial strains are also developing r ...201829241121
the attractiveness of the gravid aedes trap to dengue vectors in fiji.the gravid aedes trap (gat) is a passive trap that relies on visual and olfactory cues to lure and capture gravid mosquitoes. the gat was designed as a dengue vector surveillance tool for use in conditions where power sources are not readily available. experiments were conducted over a 10-d period in fiji to determine which species of mosquitoes the gat would collect, how different infusion types affect the attractiveness of the trap, how long each infusion type took to begin attracting mosquito ...201729244174
zika virus can be venereally transmitted between aedes aegypti mosquitoes.alternative transmission routes have been described for zika virus (zikv). here, we assessed for the first time the venereal transmission of zikv between aedes aegypti under laboratory conditions.201729246237
aedes aegypti microrna mir-2b regulates ubiquitin-related modifier to control chikungunya virus replication.arboviruses that replicate in mosquitoes activate innate immune response within mosquitoes. regulatory non-coding micrornas (mirna) are known to be modulated in mosquitoes during chikungunya infection. however, information about targets of these mirnas is scant. the present study was aimed to identify and analyze targets of mirnas that are regulated during chikungunya virus (chikv) replication in aedes aegypti cells and in the mosquito. employing next-generation sequencing technologies, we ident ...201729247247
green synthesis of silver nanoparticles using holarrhena antidysenterica (l.) wall.bark extract and their larvicidal activity against dengue and filariasis vectors.the present study was carried out to evaluate the larvicidal potential of methanol, hexane, acetone, chloroform, and aqueous bark extracts of holarrhena antidysenterica (l.) wall. and silver nanoparticles (agnps) synthesized using aqueous bark extract against the third instar larvae of aedes aegypti l. and culex quinquefasciatus say. agnps were prepared by adding 10 ml of aqueous bark extract in 90 ml of 1 mm silver nitrate (agno3) solution. after 5 min of mixing, a change in color from yellow t ...201829250727
larvicidal potential of juglans regia male flower against anopheles stephensi, aedes aegypti and culex quinquefasciatus.the diseases vectored by mosquitoes continue to be a main cause of illnesses and death throughout the world. the methanol extract of juglans regia male flower was screened for larvicidal activity against three therapeutically important mosquito vectors viz., malarial vector, anopheles stephensi; dengue vector, aedes aegypti and the filarial vector, culex quinquefasciatus. the larvicidal activity was assayed against the early fourth-instar larvae of tested mosquito species at a concentration rang ...201729251995
aedes aegypti resistance development to commonly used insecticides in jakarta, indonesia.aedes aegypti is the primary vector of various relevant arthropod-borne viral infectious diseases worldwide. the mosquito control is still mainly performed by using insecticides but their effectiveness is increasingly questioned nowadays. we here conducted a study on ae. aegypti resistance development towards several commonly used insecticides in the capital city of jakarta, indonesia. in order to achieve this goal, ae. aegypti eggs from jakarta were collected with ovitraps and hatched in the in ...201729253003
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