Publications

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proteomics informed by transcriptomics for characterising active transposable elements and genome annotation in aedes aegypti.aedes aegypti is a vector for the (re-)emerging human pathogens dengue, chikungunya, yellow fever and zika viruses. almost half of the ae. aegypti genome is comprised of transposable elements (tes). transposons have been linked to diverse cellular processes, including the establishment of viral persistence in insects, an essential step in the transmission of vector-borne viruses. however, up until now it has not been possible to study the overall proteome derived from an organism's mobile geneti ...201728103802
mitigating diseases transmitted by aedes mosquitoes: a cluster-randomised trial of permethrin-impregnated school uniforms.viral diseases transmitted via aedes mosquitoes are on the rise, such as zika, dengue, and chikungunya. novel tools to mitigate aedes mosquitoes-transmitted diseases are urgently needed. we tested whether commercially insecticide-impregnated school uniforms can reduce dengue incidence in school children.201728103255
ecotoxicological effects of larvicide used in the control of aedes aegypti on nontarget organisms: redefining the use of pyriproxyfen.the continued widespread use of larvicides in aedes aegypti control programs is still a necessary strategy, since there are no apparent efficient vaccines against arboviruses. however, chemical approaches may affect nontarget organisms and produce detrimental effects to environmental health. therefore, the aim of this study was to conduct toxicity testing for pyriproxyfen at different concentrations using daphnia magna and artemia salina as model organisms to evaluate the ecotoxicological parame ...201728095184
excito-repellency of citrus hystrix dc leaf and peel essential oils against aedes aegypti and anopheles minimus (diptera: culicidae), vectors of human pathogens.the essential oils of kaffir lime (citrus hystrix dc.) at four different concentrations (0.5, 1.0, 2.5, and 5.0% v/v) were studied for their repellency, excitation, and knockdown properties against laboratory strains of aedes aegypti (l.) and anopheles minimus theobald using an excito-repellency test system. both contact and noncontact escape responses to leaf- and peel-derived kaffir lime oils were observed. comparing unadjusted escape responses for an. minimus, leaf oil had strong combined irr ...201728082646
toxicity and larvicidal activity of podophyllum-based lignans against aedes aegypti (diptera: culicidae).aedes aegypti l. (diptera: culicidae) is a mosquito species that has adapted to urban environments and is the main vector of dengue viruses. because of the increasing incidence of dengue, a more environmentally acceptable insecticide needs to be found. natural products have been and continue to be an important source of leading compounds that can be modified in order to develop new drugs. the lignan family of natural products includes compounds with a diverse spectrum of biological activity. pod ...201728082643
evaluation of clove oil, icaridin, and transfluthrin for spatial repellent effects in three tests systems against the aedes aegypti (diptera: culicidae).one essential oil (clove oil), one skin repellent (icaridin), and one insecticide (transfluthrin) were tested for spatial repellent effects against non-blood-fed female aedes aegypti (l.) mosquitoes. the compounds were tested in acetone dilution series using a y-olfactometer, a double cage system, and a double room system. all compounds exhibited spatial repellent effects at certain concentrations. clove oil required relative high dosages to cause high effects (y-olfactometer 6 mg, double cage 6 ...201728082642
egg hatching and survival of immature stages of aedes aegypti (diptera: culicidae) under natural temperature conditions during the cold season in buenos aires, argentina.in temperate regions, the seasonal dynamics of aedes aegypti (l.) (diptera: culicidae) is mainly influenced by temperature. it is assumed that, during the winter season, the population remains as eggs and that the development and population growth of surviving eggs begin during the following spring. the aim of the current study was to assess egg hatching of ae. aegypti during the winter in buenos aires city (argentina), and analyze the survival of immature stages. the experiments consisted of im ...201728082637
learning in mosquito larvae (aedes aegypti): habituation to a visual danger signal.in spite of the mosquito aedes aegypti being a vector of several infectious diseases, a limited number of studies has been undertaken on learning in this species. moreover, larval stages have been neglected as model organisms, although they are active, aquatic and perform stereotyped behavioural responses, e.g. the escape response when disturbed. to study the learning abilities of mosquito larvae, we focused on habituation, a form of non-associative learning widely studied in vertebrates and inv ...201728077263
rna interference for mosquito and mosquito-borne disease control.rna interference (rnai) is a powerful tool to silence endogenous mosquito and mosquito-borne pathogen genes in vivo. as the number of studies utilizing rnai in basic research grows, so too does the arsenal of physiological targets that can be developed into products that interrupt mosquito life cycles and behaviors and, thereby, relieve the burden of mosquitoes on human health and well-being. as this technology becomes more viable for use in beneficial and pest insect management in agricultural ...201728067782
the antiviral rnai response in vector and non-vector cells against orthobunyaviruses.vector arthropods control arbovirus replication and spread through antiviral innate immune responses including rna interference (rnai) pathways. arbovirus infections have been shown to induce the exogenous small interfering rna (sirna) and piwi-interacting rna (pirna) pathways, but direct antiviral activity by these host responses in mosquito cells has only been demonstrated against a limited number of positive-strand rna arboviruses. for bunyaviruses in general, the relative contribution of sma ...201728060823
bombyx mori and aedes aegypti form multi-functional immune complexes that integrate pattern recognition, melanization, coagulants, and hemocyte recruitment.the innate immune system of insects responds to wounding and pathogens by mobilizing multiple pathways that provide both systemic and localized protection. key localized responses in hemolymph include melanization, coagulation, and hemocyte encapsulation, which synergistically seal wounds and envelop and destroy pathogens. to be effective, these pathways require a targeted deposition of their components to provide protection without compromising the host. extensive research has identified a larg ...201728199361
meteorological variables and mosquito monitoring are good predictors for infestation trends of aedes aegypti, the vector of dengue, chikungunya and zika.aedes aegypti is an important vector for arboviroses and widely distributed throughout the world. climatic factors can influence vector population dynamics and, consequently, disease transmission. the aim of this study was to characterize the temporal dynamics of an ae. aegypti population and dengue cases and to investigate the relationship between meteorological variables and mosquito infestation.201728193291
prevalence of dengue and diversity of cultivable bacteria in vector aedes aegypti (l.) from two dengue endemic districts, kanchanpur and parsa of nepal.dengue fever, an endemic arboviral disease, represents one of the major public health concerns in nepal. it is transmitted by bites of infected aedes aegypti and aedes albopictus, the former being primary vector. the bacterial community plays a significant role in biology of mosquitoes; however, the bacterial communities of primary vector a. aegypti remain unstudied in nepal. the study was designed to determine the rate of dengue seropositivity and to explore the bacterial diversity of a. aegypt ...201728193261
leptolegnia chapmanii (straminipila: peronosporomycetes) as a future biorational tool for the control of aedes aegypti (l.).the aim of the present review is to summarize the current knowledge about leptolegnia chapmanii as a pathogen of mosquito larvae. to this end, we present data on its identification, distribution, host range and effects on non-target organisms, effects of environmental factors, in vitro growth, release and persistence in anthropic environments, and effect combined with other insecticides. the data presented allow confirming its potential as a biocontrol agent.201728188766
zika and chikungunya: mosquito-borne viruses in a changing world.the reemergence and growing burden of mosquito-borne virus infections have incited public fear and growing research efforts to understand the mechanisms of infection-associated health outcomes and to provide better approaches for mosquito vector control. while efforts to develop therapeutics, vaccines, and novel genetic mosquito-control technologies are underway, many important underlying ecological questions remain that could significantly enhance our understanding and ability to predict and pr ...201728187236
aedes aegypti larvicidal sesquiterpene alkaloids from maytenus oblongata.four new sesquiterpene alkaloids (1-4) with a β-dihydroagrofuran skeleton and a new triterpenoid (5) were isolated from an ethyl acetate extract of maytenus oblongata stems. their structures were elucidated using 1d and 2d nmr spectroscopy as well as ms and ecd experiments. the m. oblongata stem etoac extract and the pure compounds isolated were tested for larvicidal activity against aedes aegypti under laboratory conditions, and compounds 2 and 3 were found to be active.201728186749
single molecule rna sequencing uncovers trans-splicing and improves annotations in anopheles stephensi.single molecule real-time (smrt) sequencing has recently been used to obtain full-length cdna sequences that improve genome annotation and reveal rna isoforms. here, we used one such method called isoform sequencing from pacific biosciences (pacbio) to sequence a cdna library from the asian malaria mosquito anopheles stephensi. more than 600 000 full-length cdnas, referred to as reads of insert, were identified. owing to the inherently high error rate of pacbio sequencing, we tested different ap ...201728181326
xanthine dehydrogenase-1 silencing in aedes aegypti mosquitoes promotes a blood feeding-induced adulticidal activity.aedesaegypti has 2 genes encoding xanthine dehydrogenase (xdh). we analyzed xdh1 and xdh2 gene expression by real-time quantitative pcr in tissues from sugar- and blood-fed females. differential xdh1 and xdh2 gene expression was observed in tissues dissected throughout a time course. we next exposed females to blood meals supplemented with allopurinol, a well-characterized xdh inhibitor. we also tested the effects of injecting double-stranded rna (dsrna) against xdh1, xdh2, or both. disruption o ...201728179423
mosquitocidal activity and mode of action of the isoxazoline fluralaner.mosquitoes, such as aedes aegypti and anopheles gambiae, are important vectors of human diseases. fluralaner, a recently introduced parasiticide, was evaluated as a mosquitocide in this study. on ae. aegypti and an. gambiae fourth-instar larvae, fluralaner had 24-h lc50 (lethal concentration for 50% mortality) values of 1.8 ppb and 0.4 ppb, respectively. following topical application to adult ae. aegypti, fluralaner toxicity reached a plateau in about 3 days, with 1- and 3-day ld50 (lethal dose ...201728178191
sweet waste extract uptake by a mosquito vector: survival, biting, fecundity responses, and potential epidemiological significance.in nature, adult mosquitoes typically utilize nectar as their main energy source, but they can switch to other as yet unidentified sugary fluids. contemporary lifestyles, with their associated unwillingness to consume leftovers and improper disposal of waste, have resulted in the disposal of huge amounts of waste into the environment. such refuse often contains unfinished food items, many of which contain sugar and some of which can collect water from rain and generate juices. despite evidence t ...201728174057
reducing biting rates of aedes aegypti with metofluthrin: investigations in time and space.indoor residual spraying is key to dengue control in cairns and other parts of northern queensland, australia, where aedes aegypti is prevalent, but the strategy faces challenges with regards to slow application time and, therefore, community coverage. a faster potential improvement might be the use of polyethylene netting impregnated with the volatile pyrethroid metofluthrin (sumione™). this formulation was assessed in rooms in three houses in cairns, australia. one emanator was placed in each ...201728173824
modeling the flux of metabolites in the juvenile hormone biosynthesis pathway using generalized additive models and ordinary differential equations.juvenile hormone (jh) regulates development and reproductive maturation in insects. the corpora allata (ca) from female adult mosquitoes synthesize fluctuating levels of jh, which have been linked to the ovarian development and are influenced by nutritional signals. the rate of jh biosynthesis is controlled by the rate of flux of isoprenoids in the pathway, which is the outcome of a complex interplay of changes in precursor pools and enzyme levels. a comprehensive study of the changes in enzymat ...201728158248
expression of heat shock proteins (hsps) in aedes aegypti (l) and aedes albopictus (skuse) (diptera: culicidae) larvae in response to thermal stress.climatic changes are responsible, to a certain extent for the occurrence and spread of arboviral pathogens world over. temperature is one of the important abiotic factors influencing the physiological processes of mosquitoes. several genes of heat shock protein (hsp) families are known to be expressed in mosquitoes, which aid in overcoming stress induced by elevated temperature. in order to understand expression of hsp family genes in the andaman population of aedes aegypti and aedes albopictus, ...201728024869
mosquito repellent activity of volatile oils from selected aromatic plants.essential oils from fresh leaves of four aromatic plants viz., ocimum sanctum, mentha piperita, eucalyptus globulus and plectranthus amboinicus were extracted by hydrodistillation. the test solutions were prepared as 20% essential oil in ethanol and positive control as 20% deet in ethanol. essential oil blend was prepared as 5% concentration. nulliparous, 3-5-day-old female adult aedes aegypti mosquitoes were used for repellency screening as per icmr protocol. the study showed that the repellenc ...201728013374
influence of the length of storage on aedes aegypti (diptera: culicidae) egg viability.aedes aegypti (l.) is one of the most important arboviral vectors worldwide. vector control is targeted at immature and adult stages; however, eggs are resistant to desiccation and may repopulate treated areas long after treatment ceases. we investigated the effect of age on ae. aegypti egg hatching rates using newly colonized populations (f2) from an arid region. we found a strongly negative association where older eggs had lower hatch rates. the capacity of eggs to survive for long periods of ...201728011737
indoor resting behavior of aedes aegypti (diptera: culicidae) in acapulco, mexico.the markedly anthropophilic and endophilic behaviors of aedes aegypti (l.) make it a very efficient vector of dengue, chikungunya, and zika viruses. although a large body of research has investigated the immature habitats and conditions for adult emergence, relatively few studies have focused on the indoor resting behavior and distribution of vectors within houses. we investigated the resting behavior of ae. aegypti indoors in 979 houses of the city of acapulco, mexico, by performing exhaustive ...201728011725
toxicity and synergistic activities of chalcones against aedes aegypti (diptera: culicidae) and drosophila melanogaster (diptera: drosophilidae).mosquito-borne illnesses are of great concern throughout the world, and chemical insecticides are commonly employed to decrease mosquito populations. however, the developmental insecticide pipeline for vector control has primarily been filled by repurposed agricultural products, and is hampered by their widespread use and insecticide resistance. the present study was performed in the search for new chemical insecticides or insecticide synergists. screening of 31 chalcone analogs was performed us ...201728011724
exon-enriched libraries reveal large genic differences between aedes aegypti from senegal, west africa, and populations outside africa.aedes aegypti is one of the most studied mosquito species, and the principal vector of several arboviruses pathogenic to humans. recently failure to oviposit, low fecundity, and poor egg-to-adult survival were observed when ae. aegypti from senegal (senaae) west africa were crossed with ae. aegypti (aaa) from outside of africa, and in senaae intercrosses. fluorescent in situ hybridization analyses indicated rearrangements on chromosome 1, and pericentric inversions on chromosomes 2 and 3. herein ...201728007834
in vitro evaluation of the effect of botanical formulations used in the control of aedes aegypti l. (diptera: culicidae) on liver enzymes.dengue fever is a viral disease transmitted by the aedes aegypti linn. (1792) (diptera: culicidae) mosquito, which is endemic in several regions of brazil. alternative methods for the control of the vector include botanical insecticides, which offer advantages such as lower environmental contamination levels and less likelihood of resistant populations. thus, in this study, the ability of botanical insecticide formulations to inhibit the activity of the liver enzymes serum cholinesterase and mal ...201728001215
a biodegradable device for the controlled release of piper nigrum (piperaceae) standardized extract to control aedes aegypti (diptera, culicidae) larvae.the significant increase in dengue, zika, and chikungunya and the resistance of the aedes aegypti mosquito to major insecticides emphasize the importance of studying alternatives to control this vector. the aim of this study was to develop a controlled-release device containing piper nigrum extract and to study its larvicidal activity against aedes aegypti.201728001214
aedes aegypti rhesus glycoproteins contribute to ammonia excretion by larval anal papillae.in larval aedes aegypti, transcripts of the rhesus-like glycoproteins aerh50-1 and aerh50-2 have been detected in the anal papillae, sites of ammonia (nh3/nh4(+)) excretion; however, these putative ammonia transporters have not been previously localized or functionally characterized. in this study, we show that the aerh50s co-immunolocalize with apical v-type h(+)-atpase as well as with basal na(+)/k(+)-atpase in the epithelium of anal papillae. the double-stranded rna-mediated knockdown of aerh ...201727885043
long-term spatio-temporal dynamics of the mosquito aedes aegypti in temperate argentina.buenos aires city is located near the southern limit of the distribution of aedes aegypti (diptera: culicidae). this study aimed to assess long-term variations in the abundance of ae. aegypti in buenos aires in relation to changes in climatic conditions. ae. aegypti weekly oviposition activity was analyzed and compared through nine warm seasons from 1998 to 2014, with 200 ovitraps placed across the whole extension of the city. the temporal and spatial dynamics of abundances were compared among s ...201727876100
long-lasting permethrin-impregnated clothing: protective efficacy against malaria in hyperendemic foci, and laundering, wearing, and weathering effects on residual bioactivity after worst-case use in the rain forests of french guiana.personal protective measures against hematophagous vectors constitute the first line of defense against arthropod-borne diseases. however, guidelines for the standardized testing and licensing of insecticide-treated clothing are still lacking. the aim of this study was to analyze the preventive effect of long-lasting polymer-coated permethrin-impregnated clothing (ptbdu) against malaria after exposure to high-level disease transmission sites as well as the corresponding loss of permethrin and bi ...201727942961
impact of terminalia chebula retz. against aedes aegypti l. and non-target aquatic predatory insects.aedes aegypti linn is one of the most important mosquito species. the vectors are responsible for causing deadly diseases like dengue and dengue hemorrhagic fever. several chemical pesticides used to control these dengue vectors caused severe toxic significances on human health and other non-target beneficial insects. therefore the current investigation has been made to access the bio-efficacy of the crude seed extracts of t. chebula against the dengue vector ae. aegypti. the gc-ms analysis of c ...201727940415
establishment of aedes aegypti (l.) in mountainous regions in mexico: increasing number of population at risk of mosquito-borne disease and future climate conditions.the study was conducted in the central region of veracruz mexico, in the metropolitan area of xalapa. it is a mountainous area where aedes aegypti (l.) is not currently endemic. an entomological survey was done along an elevation gradient using the ae. aegypti occurrences at different life cycle stages. seven sites were sampled and a total of 24 mosquito species were recorded: 9 species were found in urban areas, 18 in non-urban areas with remnant vegetation, and 3 occurred in both environments. ...201727863974
the formulation of the essential oil of piper aduncum linnaeus (piperales: piperaceae) increases its efficacy as an insect repellent.the essential oil (eo) of piper aduncum linnaeus, known as 'sireh lada' to locals malaysian, has the potential to be used as an alternative to synthetic insect repellents such as n,n-diethyl-meta-toluamide. however, the eo's efficacy as a repellent decreases after application due to the high volatility of its active ingredients. a number of studies have showed that optimizing the formulation of plant-based eos can improve their efficacy as repellents. the present study sought to evaluate the eff ...201727819201
mosquito populations dynamics associated with climate variations.mosquitoes are responsible for the transmission of numerous serious pathogens. members of the aedes and culex genera, which include many important vectors of mosquito-borne diseases, are highly invasive and adapted to man-made environments. they are spread around the world involuntarily by humans and are highly adapted to urbanized environments, where they are exposed to climate-related abundance drivers. we investigated culicidae fauna in two urban parks in the city of são paulo to analyze the ...201727810426
repellency and larvicidal activity of essential oils from xylopia laevigata, xylopia frutescens, lippia pedunculosa, and their individual compounds against aedes aegypti linnaeus.in order to find new alternatives for vector control and personal protection, we evaluated the larvicidal and repellent activity of essentials oils from plants found in the northeast of brazil against aedes aegypti linnaeus mosquitoes. the plants tested include xylopia laevigata, xylopia frutescens, and lippia pedunculosa and their major compounds, piperitenone oxide, and (r)-limonene. the essential oil of l. pedunculosa and its major volatile compounds were shown to be toxic for ae. aegypti lar ...201727844468
dynamic expression of genes encoding subunits of inward rectifier potassium (kir) channels in the yellow fever mosquito aedes aegypti.inward rectifier potassium (kir) channels play fundamental roles in neuromuscular, epithelial, and endocrine function in mammals. recent research in insects suggests that kir channels play critical roles in the development, immune function, and excretory physiology of fruit flies and/or mosquitoes. moreover, our group has demonstrated that mosquito kir channels may serve as valuable targets for the development of novel insecticides. here we characterize the molecular expression of 5 mrnas encodi ...201727836744
essential oils enhance the toxicity of permethrin against aedes aegypti and anopheles gambiae.insecticide resistance and growing public concern over the safety and environmental impacts of some conventional insecticides have resulted in the need to discover alternative control tools. naturally occurring botanically-based compounds are of increased interest to aid in the management of mosquitoes. susceptible strains of aedes aegypti (linnaeus) (diptera: culicidae) and anopheles gambiae (meigen) (diptera: culicidae) were treated with permethrin, a common type-i synthetic pyrethroid, using ...201727800630
how much does inbreeding reduce heterozygosity? empirical results from aedes aegypti.deriving strains of mosquitoes with reduced genetic variation is useful, if not necessary, for many genetic studies. inbreeding is the standard way of achieving this. full-sib inbreeding the mosquito aedes aegypti for seven generations reduced heterozygosity to 72% of the initial heterozygosity in contrast to the expected 13%. this deviation from expectations is likely due to high frequencies of deleterious recessive alleles that, given the number of markers studied (27,674 single nucleotide pol ...201727799643
salinity responsive aquaporins in the anal papillae of the larval mosquito, aedes aegypti.the larvae of the mosquito, aedes aegypti normally inhabit freshwater (fw) where they face dilution of body fluids by osmotic influx of water. in response, the physiological actions of the anal papillae result in ion uptake while the malpighian tubules and rectum work in concert to excrete excess water. in an apparent paradox, the anal papillae express aquaporins (aqps) and are sites of water permeability which, if aqps are expressed by the epithelium, apparently exaggerates the influx of water ...201727642095
salinity alters snakeskin and mesh transcript abundance and permeability in midgut and malpighian tubules of larval mosquito, aedes aegypti.this study examined the distribution and localization of the septate junction (sj) proteins snakeskin (ssk) and mesh in osmoregulatory organs of larval mosquito (aedes aegypti), as well as their response to altered environmental salt levels. ssk and mesh transcripts and immunoreactivity were detected in tissues of endodermal origin such as the midgut and malpighian tubules of a. aegypti larvae, but not in ectodermally derived hindgut and anal papillae. immunolocalization of ssk and mesh in the m ...201727988380
diverse subcellular localizations of the insect cmp-sialic acid synthetases.the occurrence and biological importance of sialic acid (sia) and its metabolic enzymes in insects have been studied using drosophila melanogaster. the most prominent feature of d. melanogaster cmp-sia synthetase (dmcss) is its golgi-localization, contrasted with nuclear localization of vertebrate csss. however, it remains unclear if the golgi-localization is common to other insect csss and why it happens. to answer these questions, aedes aegypti (mosquito) css (aacss) and tribolium castaneum (b ...201727986833
field comparisons of the gravid aedes trap (gat) and bg-sentinel trap for monitoring aedes albopictus (diptera: culicidae) populations and notes on indoor gat collections in vietnam.we report on the use of the gravid aedes trap (gat) as a surveillance device for aedes albopictus (skuse) relative to the bg-sentinel (bgs) trap in field studies conducted in trenton, nj, and on hammond island, queensland, australia. a parallel study conducted in nha trang, vietnam, assessed the use of the gat as an indoor surveillance device as well as the use of canola oil as a noninsecticide killing agent. in trenton and hammond island, the gat collected fewer male (0.40 ± 0.12 and 0.43 ± 0.3 ...201727707983
impact of micropollutants on the life-history traits of the mosquito aedes aegypti: on the relevance of transgenerational studies.hazard assessment of chemical contaminants often relies on short term or partial life-cycle ecotoxicological tests, while the impact of low dose throughout the entire life cycle of species across multiple generations has been neglected. this study aimed at identifying the individual and population-level consequences of chronic water contamination by environmental concentrations of three organic micropollutants, ibuprofen, bisphenol a and benzo[a]pyrene, on aedes aegypti mosquito populations in e ...201727667679
changes in infestation sites of female aedes aegypti in northeast brazil.we report the behavioral changes in use of oviposition sites in aedes aegypti in fortaleza, brazil.201727598639
cost of intensive routine control and incremental cost of insecticide-treated curtain deployment in a setting with low aedes aegypti infestation.information regarding the cost of implementing insecticide-treated curtains (itcs) is scarce. therefore, we evaluated the itc implementation cost, in addition to the costs of intensive conventional routine activities of the aedes control program in the city of guantanamo, cuba.201727598627
molecular docking and simulation studies of gustatory receptor of aedes aegypti: a potent drug target to distract host-seeking behaviour in mosquitoes.it is well reported that exhaled co 2 and skin odour from human being assist female mosquitoes to locate human host. basically, the receptors for this activity are expressed in cpa neurons. in both aedes aegypti and anopheles gambiae, this co 2-sensitive olfactory neuron detects myriad number of chemicals present in human skin. therefore, manipulation of gustatory receptors housing these neurons may serve as important targets for behavioural intervention. the study was aimed towards virtual scre ...201727353589
impact of container material on the development of aedes aegypti larvae at different temperatures.aedes aegypti, the primary vector of dengue generally breeds in intradomestic and peridomestic containers made up of different materials, i.e. plastic, iron, rubber, earthen material etc. the material of container is likely to affect the temperature of water in container with variation in environmental temperature. the present study was aimed to determine the effect of different container materials on larval development of ae. aegypti at different temperatures.201727353584
spatial distribution and insecticide susceptibility status of aedes aegypti and aedes albopictus in dengue affected urban areas of rawalpindi, pakistan.dengue is one of the most common arthropod-borne viral diseases which is transmitted mainly by two vector species, aedes aegypti (linnaeus 1762) and ae. albopictus (skuse, 1894) worldwide. as there is no effective medicine and vaccine available, vector control remains the most effective measure to prevent its transmission and outbreak. the aim of the study was to confirm the co-occurrence of aedes aegypti and ae. albopictus populations in the different localities of rawalpindi, pakistan and exam ...201727353583
spatial analysis of wing geometry in dengue vector mosquito, aedes aegypti (l.) (diptera: culicidae), populations in metropolitan manila, philippines.aedes aegypti (l.) is an efficient vector for arboviral diseases such as dengue. the wings of ae. aegypti has been extensively studied in order to investigate population heterogeneity and structure by utilizing a landmark based geometric morphometrics (gms) approach. the aim of this study was to examine and assess the wing geometry of ae. aegypti in metropolitan manila.201727353582
trypsin inhibitor from leucaena leucocephala seeds delays and disrupts the development of aedes aegypti, a multiple-disease vector.nowadays, the aedes aegypti mosquito represents a serious public health issue in view of the large outbreaks of the arboviral diseases zika, dengue, chikungunya and yellow fever. this holometabolous insect has midgut digestive enzymes that are trypsin- and chymotrypsin-like proteins. protease inhibitors are able to bind to proteolytic enzymes and promote a blockage in digestion and nutrition, leading to death. thus, we investigated the effect of trypsin inhibitor of leucaena leucocephala (lti) s ...201727040615
synthesis and insecticidal activity of novel pyrimidine derivatives containing urea pharmacophore against aedes aegypti.aedes aegypti is a major mosquito vector for the transmission of serious diseases, especially dengue and yellow fever. more than 1 billion people in developing countries are at risk. the widespread and continual use of pesticides can lead to resistant mosquitoes. in order to maintain mosquito control gains, it is critical to develop and evaluate novel bioactive molecules that differ in mode of action from currently used products.201727448764
aedes aegypti (diptera: culicidae) in a tree hole in brazil. 201727384845
serious disease outbreaks caused by viruses transmitted by aedes aegypti in brazil. 201727384820
sexual chemoecology of mosquitoes (diptera, culicidae): current knowledge and implications for vector control programs.mosquitoes (diptera: culicidae) act as vectors of medical and veterinary importance, due to their ability to transmit many pathogens and parasites. renewed interest has been recently devoted to the potential of sterile insect technique (sit) for mosquito suppression. however, the success of the sit is mostly dependent on the ability of sterile males to compete for mates with the wild ones in the field. nevertheless, little is known on the sexual chemical ecology of mosquitoes, with special refer ...201727692501
toxicity of squamocin on aedes aegypti larvae, its predators and human cells.the mosquito aedes aegypti transmits a virus that causes diverse human diseases, and control of the vector is an important strategy to avoid disease propagation. plants in the family annonaceae are recognised as sources of molecules with uses in the medical and agriculture fields. molecules of secondary metabolites of annonaceae plants exhibit insecticidal potential against insect pests and vectors, especially acetogenins, showing high toxicity at low doses, which has encouraged research into pr ...201727366879
[control of aedes aegypti breeding sites with the program recicla por tu bienestar in merida, mexico].to determine the importance of ae. aegypti breeding-sites in merida;to evaluate the impact of recicla por tu bienestar (rxb, a recycling program) on the reduction of breeding sites and the perception of participants.201726302122
transposition of mutator-like transposable elements (mules) resembles hat and transib elements and v(d)j recombination.mutator-like transposable elements (mules) are widespread across fungal, plant and animal species. despite their abundance and importance as genetic tools in plants, the transposition mechanism of the mule superfamily was previously unknown. discovery of the muta1 element from aedes aegypti and its successful transposition in yeast facilitated the characterization of key steps in muta1 transposition. here we show that purified transposase binds specifically to the muta1 ends and catalyzes excisi ...201728482040
ascogregarina taiwanensis infection in aedes aegypti and aedes albopictus in santa catarina, south brazil.this study registers ascogregarina spp. infection in field populations of aedes aegypti and aedes albopictus in a subtropical region of brazil.201728562761
molecular characterization of the gene profile of bacillus thuringiensis berliner isolated from brazilian ecosystems and showing pathogenic activity against mosquito larvae of medical importance.the occurrence of aedes aegypti, culex quinquefasciatus, and mosquitoes of the genus anopheles potentiate the spread of several diseases, such as dengue, zika, chikungunya, urban yellow fever, filariasis, and malaria, a situation currently existing in brazil and in latin america. control of the disease vectors is the most effective tool for containing the transmission of the pathogens causing these diseases, and the bacterium bacillus thuringiensis var. israelensis has been widely used and has s ...201728823909
mosquitocidal potential of silver nanoparticles synthesized using local isolates of bacillus thuringiensis subsp. israelensis and their synergistic effect with a commercial strain of b. thuringiensis subsp. israelensis.control of larval stages of aedes aegypti is considered an effective approach for preventing outbreaks of dengue fever. in this work, silver nanoparticles (ag nps) were synthesized using the supernatant and insecticidal proteins from local isolates of bacillus thuringiensis subsp. israelensis (bti). mosquitocidal activity assays against a. aegypti larvae revealed that the highest toxicity was obtained from the ag nps synthesized using supernatant of bti k55 and the inclusion proteins of bti k46 ...201728754251
toward mosquito control with a green alga: expression of cry toxins of bacillus thuringiensis subsp. israelensis (bti) in the chloroplast of chlamydomonas.we are developing chlamydomonas strains that can be used for safe and sustainable control of mosquitoes, because they produce proteins from bacillus thuringiensis subsp. israelensis (bti) in the chloroplast. chlamydomonas has a number of advantages for this approach, including genetic controls that are not generally available with industrial algae. the bti toxin has been used for mosquito control for > 30 years and does not engender resistance; it contains three cry proteins, cry4aa (135 kda), c ...201728713202
molecular characterization of indigenous bacillus thuringiensis strains isolated from kashmir valley.bacillus thuringiensis (bt) being an eco-friendly bioinsecticide is effectively used in pest management strategies and, therefore, isolation and identification of new strains effective against a broad range of target pests is important. in the present study, new indigenous b. thuringiensis strains were isolated and investigated so that these could be used as an alternative and/or support the current commercial strains/cry proteins in use. for this, 159 samples including soil, leaf and spider web ...201728597156
functional characterization of aedes aegypti alkaline phosphatase alp1 involved in the toxicity of cry toxins from bacillus thuringiensis subsp. israelensis and jegathesan.presently three major groups of proteins from aedes aegypti, cadherin, alkaline phosphatases (alp) and aminopeptidases n (apn), have been identified as cry11aa toxin receptors. to further characterize their role on toxicity, transgenic mosquitoes with silenced aedes cadherin expression were previously generated and the role of cadherin in mediating the toxicity of four different mosquitocidal toxins (cry11aa, cry11ba, cry4aa and cry4ba) was demonstrated. here, we investigated the role of another ...201728587836
evaluating the vector control potential of the in2care® mosquito trap against aedes aegypti and aedes albopictus under semifield conditions in manatee county, florida.successful integrated vector management programs may need new strategies in addition to conventional larviciding and adulticiding strategies to target aedes aegypti and ae. albopictus, which can develop in small, often cryptic, artificial and natural containers. the in2care® mosquito trap was recently developed to target and kill larval and adult stages of these invasive container-inhabiting aedes mosquitoes by utilizing autodissemination. gravid females that visit the trap pick up pyriproxyfen ...201728854105
mosquitoes can harbour yeasts of clinical significance and contribute to their environmental dissemination.there is still a lack of studies on fungal microbiota in mosquitoes, compared with the number available on bacterial microbiota. this study reports the identification of yeasts of clinical significance in laboratory mosquito species: anopheles gambiae, anopheles stephensi, culex quinquefasciatus, aedes albopictus and aedes aegypti. among the yeasts isolated, they focused on the opportunistic pathogen candida parapsilosis, since there is a need to better understand breakthrough candidaemia with r ...201728714286
vector competence of populations of aedes aegypti from three distinct cities in kenya for chikungunya virus.in april, 2004, chikungunya virus (chikv) re-emerged in kenya and eventually spread to the islands in the indian ocean basin, south-east asia, and the americas. the virus, which is often associated with high levels of viremia in humans, is mostly transmitted by the urban vector, aedes aegypti. the expansion of chikv presents a public health challenge both locally and internationally. in this study, we investigated the ability of ae. aegypti mosquitoes from three distinct cities in kenya; mombasa ...201728820881
co-infection of mosquitoes with chikungunya and dengue viruses reveals modulation of the replication of both viruses in midguts and salivary glands of aedes aegypti mosquitoes.arthropod-borne virus (arbovirus) infections cause several emerging and resurgent infectious diseases in humans and animals. chikungunya-affected areas often overlap with dengue-endemic areas. concurrent dengue virus (denv) and chikungunya virus (chikv) infections have been detected in travelers returning from regions of endemicity. chikv and denv co-infected aedes albopictus have also been collected in the vicinity of co-infected human cases, emphasizing the need to study co-infections in mosqu ...201728777313
high-resolution structure of a kazal-type serine protease inhibitor from the dengue vector aedes aegypti.blood-feeding exoparasites are rich sources of protease inhibitors, and the mosquito aedes aegypti, which is a vector of dengue virus, yellow fever virus, chikungunya virus and zika virus, is no exception. aati is a single-domain, noncanonical kazal-type serine proteinase inhibitor from a. aegypti that recognizes both digestive trypsin-like serine proteinases and the central protease in blood clotting, thrombin, albeit with an affinity that is three orders of magnitude lower. here, the 1.4 å res ...201728777090
evidence of dengue and chikungunya virus co-infection and circulation of multiple dengue serotypes in a recent indian outbreak.in india, dengue endemic areas overlap with chikungunya-affected areas and both the viruses are transmitted by same vector, aedes aegypti - thereby increasing opportunity of co-infection by both viruses. present study was carried out to understand the denv-chikv infection dynamics during recent outbreaks in eastern india (west bengal state) and its implication on disease manifestations. blood was collected from 326 symptomatic febrile patients. patients' serum was subjected to serological diagno ...201728756561
transmission risk of two chikungunya lineages by invasive mosquito vectors from florida and the dominican republic.between 2014 and 2016 more than 3,800 imported human cases of chikungunya fever in florida highlight the high risk for local transmission. to examine the potential for sustained local transmission of chikungunya virus (chikv) in florida we tested whether local populations of aedes aegypti and aedes albopictus show differences in susceptibility to infection and transmission to two emergent lineages of chikv, indian ocean (ioc) and asian genotypes (ac) in laboratory experiments. all examined popul ...201728749964
assessment of the probability of autochthonous transmission of chikungunya virus in canada under recent and projected climate change.chikungunya virus (chikv) is a reemerging pathogen transmitted by aedes aegypti and aedes albopictus mosquitoes. the ongoing caribbean outbreak is of concern due to the potential for infected travelers to spread the virus to countries where vectors are present and the population is susceptible. although there has been no autochthonous transmission of chikv in canada, there is concern that both ae. albopictus and chikv will become established, particularly under projected climate change. we devel ...201728731409
the medicinal chemistry of chikungunya virus.arthropod-borne viruses (arboviruses) are an important threat to human and animal health globally. among these, zoonotic diseases account for billions of cases of human illness and millions of deaths every year, representing an increasing public health problem. chikungunya virus belongs to the genus alphavirus of the family togariridae, and is transmitted mainly by the bite of female mosquitoes of the aedes aegypti and/or a. albopictus species. the focus of this review will be on the medicinal c ...201728689975
high risk for chikungunya virus to initiate an enzootic sylvatic cycle in the tropical americas.chikungunya virus (chikv) has dispersed in the americas since 2013, and its range of distribution has overlapped large forested areas. herein, we assess vector competence of two sylvatic neotropical mosquito species, haemagogus leucocelaenus and aedes terrens, to evaluate the risk of chikv to initiate a sylvatic cycle in the continent.201728662031
first report of naturally infected aedes aegypti with chikungunya virus genotype ecsa in the americas.the worldwide expansion of new emergent arboviruses such as chikungunya and zika reinforces the importance in understanding the role of mosquito species in spreading these pathogens in affected regions. this knowledge is essential for developing effective programs based on species specificity to avoid the establishment of endemic transmission cycles sustained by the identified local vectors. although the first autochthonous transmission of chikungunya virus was described in 2014 in the north of ...201728614394
immunoglobulin-responsive chikungunya encephalitis: two case reports.chikungunya virus is an alphavirus transmitted by the mosquito aedes, mainly aedes aegypti and aedes albopictus, that can cause acute illness, mostly self-limited, characterized by fever, maculopapular rash, and disabling polyarthritis/arthralgia, with an incubation period of 1 to 12 days. chikungunya was largely regarded as a non-fatal and self-limited disease, but recently, serious cases have been reported including some with severe involvement of the nervous system, such as meningoencephaliti ...201728577289
mosquito co-infection with zika and chikungunya virus allows simultaneous transmission without affecting vector competence of aedes aegypti.zika virus (zikv) and chikungunya virus (chikv) are highly pathogenic arthropod-borne viruses that are currently a serious health burden in the americas, and elsewhere in the world. zikv and chikv co-circulate in the same geographical regions and are mainly transmitted by aedes aegypti mosquitoes. there is a growing number of case reports of zikv and chikv co-infections in humans, but it is uncertain whether co-infection occurs via single or multiple mosquito bites. here we investigate the poten ...201728570693
wmel limits zika and chikungunya virus infection in a singapore wolbachia-introgressed ae. aegypti strain, wmel-sg.zika (zikv) and chikungunya (chikv) viruses are emerging aedes-borne viruses that are spreading outside their known geographic range and causing wide-scale epidemics. it has been reported that these viruses can be transmitted efficiently by ae. aegypti. recent studies have shown that ae. aegypti when transinfected with certain wolbachia strains shows a reduced replication and dissemination of dengue (denv), chikungunya (chikv), and yellow fever (yfv) viruses. the aim of this study was to determi ...201728542240
[arboviruses also have an american dream].some arboviruses that originated in the old world have been introduced by humans into the american continent. the first of them was the yellow fever virus, coming from the west african coast with slaves in the 17th-19th centuries, followed by dengue viruses, which were always prevalent within the americas. next was thewest nile virus, introduced in new york in 1999, that spread in only a few years over the whole continent. then, chikungunya virus arrived on saint martin island in 2013 after its ...201728509947
the midgut transcriptome of aedes aegypti fed with saline or protein meals containing chikungunya virus reveals genes potentially involved in viral midgut escape.the mosquito aedes aegypti is the primary vector for medically important arthropod-borne viruses, including chikungunya virus (chikv). following oral acquisition, an arbovirus has to persistently infect several organs in the mosquito before becoming transmissible to another vertebrate host. a major obstacle an arbovirus has to overcome during its infection cycle inside the mosquito is the midgut escape barrier, representing the exit mechanism arboviruses utilize when disseminating from the midgu ...201728506207
clinical features and neurologic complications of children hospitalized with chikungunya virus in honduras.the first case of chikungunya virus in honduras was identified in 2014. the virus has spread widely across honduras via the aedes aegypti mosquito, leading to an outbreak of chikungunya virus (chikv) in 2015 that significantly impacted children. a retrospective chart review of 235 children diagnosed with chikv and admitted to the national autonomous university of honduras hospital escuela (hospital escuela) in tegucigalpa, honduras, was accomplished with patients who were assessed for clinical f ...201728459170
co-circulation of dengue virus serotypes with chikungunya virus in madhya pradesh, central india.dengue and chikungunya present with very similar signs and symptoms in the initial stage of illness and so it is difficult to distinguish them clinically. both are transmitted by aedes aegypti and aedes albopictus mosquitoes. this study was conducted with the aim to explore the co-circulation of dengue and chikungunya viruses in central india.201728607252
ensuring successful introduction of wolbachia in natural populations of aedes aegypti by means of feedback control.the control of the spread of dengue fever by introduction of the intracellular parasitic bacterium wolbachia in populations of the vector aedes aegypti, is presently one of the most promising tools for eliminating dengue, in the absence of an efficient vaccine. the success of this operation requires locally careful planning to determine the adequate number of individuals carrying the wolbachia parasite that need to be introduced into the natural population. the introduced mosquitoes are expected ...201728856446
influence of the mixtures composed of slow-release insecticide formulations against aedes aegypti mosquito larvae reared in pond water.vector borne diseases remain the major source of illness and death worldwide. aedes aegypti is the primary carrier of dengue and dengue haemorrhagic fever in many developing countries in the tropical world. because a. aegypti populations are becoming more and more resistant to conventional and non conventional insecticides, alternative strategies have to be rapidly implemented in the future for dengue vector control. the present study aimed to evaluate the larvicidal efficacy of slow-release for ...201728855810
human movement, cooperation and the effectiveness of coordinated vector control strategies.vector-borne disease transmission is often typified by highly focal transmission and influenced by movement of hosts and vectors across different scales. the ecological and environmental conditions (including those created by humans through vector control programmes) that result in metapopulation dynamics remain poorly understood. the development of control strategies that would most effectively limit outbreaks given such dynamics is particularly urgent given the recent epidemics of dengue, chik ...201728855386
a statewide survey for container-breeding mosquitoes in mississippi.container-breeding mosquitoes are important in public health due to outbreaks of zika, chikungunya, and dengue viruses. this paper documents the distribution of container-breeding mosquito species in mississippi, with special emphasis on the genus aedes. five sites in each of the 82 mississippi counties were sampled monthly between may 1 and august 31, 2016, and 50,109 mosquitoes in 14 species were collected. the most prevalent and widely distributed species found was ae. albopictus, being found ...201728854113
adulticidal susceptibility evaluation of aedes albopictus using new diagnostic doses in penang island, malaysia.insecticide-based vector control approaches are facing challenges due to the development of resistance in vector mosquitoes. therefore, a proper resistance surveillance program using baseline lethal concentrations is crucial for resistance management strategies. currently, the world health organization's (who) diagnostic doses established for aedes aegypti and anopheles species are being used to study the resistance status of aedes albopictus. in this study, we established the diagnostic doses f ...201728854111
toxicological effects of chemical constituents from piper against the environmental burden aedes aegypti liston and their impact on non-target toxicity evaluation against biomonitoring aquatic insects.dengue is the most rapidly spreading mosquito-borne viral disease in the world. the mosquito, aedes aegypti, also spreads yellow fever, chikungunya, and zika virus. as the primary vector for dengue, ae. aegypti now occurs in over 20 countries and is a serious concern with reports of increasing insecticide resistance. developing new treatments to manage mosquitoes are needed. formulation of crude volatile oil from piper betle leaves (pb-cvo) was evaluated as a potential treatment which showed lar ...201728852982
the elimination of the dengue vector, aedes aegypti, from brisbane, australia: the role of surveillance, larval habitat removal and policy.aedes aegypti (l.) (diptera: culicidae) is a highly invasive mosquito whose global distribution has fluctuated dramatically over the last 100 years. in australia the distribution of ae. aegypti once spanned the eastern seaboard, for 3,000 km north to south. however, during the 1900s this distribution markedly reduced and the mosquito disappeared from its southern range. numerous hypotheses have been proffered for this retraction, however quantitative evidence of the mechanisms driving the disapp ...201728846682
zika, chikungunya, and other emerging vector-borne viral diseases.arthropod-borne viruses (arboviruses) have a long history of emerging to infect humans, but during recent decades, they have been spreading more widely and affecting larger populations. this is due to several factors, including increased air travel and uncontrolled mosquito vector populations. emergence can involve simple spillover from enzootic (wildlife) cycles, as in the case of west nile virus accompanying geographic expansion into the americas; secondary amplification in domesticated animal ...201728846489
sero-surveillance of dengue in the city lahore, pakistan.to conduct sero-surveillance of dengue cases, and to find out the demographic and socio-economic status of dengue patients.201728839300
carryover effects of larval exposure to different environmental bacteria drive adult trait variation in a mosquito vector.conditions experienced during larval development of holometabolous insects can affect adult traits, but whether differences in the bacterial communities of larval development sites contribute to variation in the ability of insect vectors to transmit human pathogens is unknown. we addressed this question in the mosquito aedes aegypti, a major arbovirus vector breeding in both sylvatic and domestic habitats in sub-saharan africa. targeted metagenomics revealed differing bacterial communities in th ...201728835919
the quantitative structure-insecticidal activity relationships from plant derived compounds against chikungunya and zika aedes aegypti (diptera:culicidae) vector.the insecticidal activity of a series of 62 plant derived molecules against the chikungunya, dengue and zika vector, the aedes aegypti (diptera:culicidae) mosquito, is subjected to a quantitative structure-activity relationships (qsar) analysis. the replacement method (rm) variable subset selection technique based on multivariable linear regression (mlr) proves to be successful for exploring 4885 molecular descriptors calculated with dragon 6. the predictive capability of the obtained models is ...201728830053
maintaining aedes aegypti mosquitoes infected with wolbachia.aedes aegypti mosquitoes experimentally infected with wolbachia are being utilized in programs to control the spread of arboviruses such as dengue, chikungunya and zika. wolbachia-infected mosquitoes can be released into the field to either reduce population sizes through incompatible matings or to transform populations with mosquitoes that are refractory to virus transmission. for these strategies to succeed, the mosquitoes released into the field from the laboratory must be competitive with na ...201728829414
limited transmission potential of takeda's tetravalent dengue vaccine candidate by aedes albopictus.recombinant live-attenuated chimeric tetravalent dengue vaccine viruses, tdv-1, -2, -3, and -4, contain the premembrane and envelope genes of dengue virus serotypes 1-4 in the replicative background of the attenuated dengue virus type-2 (denv-2) pdk-53 vaccine strain. previous results have shown that these recombinant vaccine viruses demonstrate limited infection and dissemination in aedes aegypti and are unlikely to be transmitted by the primary mosquito vector of denvs. in this report, we expa ...201728820715
scaling up of an innovative intervention to reduce risk of dengue, chikungunya, and zika transmission in uruguay in the framework of an intersectoral approach with and without community participation.to contribute to the prevention of dengue, chikungunya, and zika, a process of scaling up an innovative intervention to reduce aedes aegypti habitats, was carried out in the city of salto (uruguay) based on a transdisciplinary analysis of the eco-bio-social determinants. the intervention in one-third of the city included the distributions of plastic bags for all households to collect all discarded water containers that were recollected by the ministry of health and the municipality vector contro ...201728820690
assessment of risk of dengue and yellow fever virus transmission in three major kenyan cities based on stegomyia indices.dengue (den) and yellow fever (yf) are re-emerging in east africa, with contributing drivers to this trend being unplanned urbanization and increasingly adaptable anthropophilic aedes (stegomyia) vectors. entomological risk assessment of these diseases remains scarce for much of east africa and kenya even in the dengue fever-prone urban coastal areas. focusing on major cities of kenya, we compared den and yf risk in kilifi county (den-outbreak-prone), and kisumu and nairobi counties (no document ...201728817563
an economic evaluation of vector control in the age of a dengue vaccine.dengue is a rapidly emerging vector-borne neglected tropical disease, with a 30-fold increase in the number of cases reported since 1960. the economic cost of the illness is measured in the billions of dollars annually. environmental change and unplanned urbanization are conspiring to raise the health and economic cost even further beyond the reach of health systems and households. the health-sector response has depended in large part on control of the aedes aegypti and ae. albopictus (mosquito) ...201728806786
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