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biological control of culicidae with the copepod mesocyclops aspericornis and larvivorous fish (poeciliidae) in a village of french polynesia.the copepod mesocyclops aspericornis daday and the larvivorous fishes gambusia affinis (b. & g.) and poecilia reticulata r. & b., were released into mosquito breeding sites in tuherahera village, tikehau atoll, french polynesia, to control larvae of aedes aegypti (l.), ae.polynesiensis marks, culex annulirostris skuse and cx quinquefasciatus say. treatments were completed within a week, in january 1990. fish quickly eliminated mosquito larvae from the open breeding sites (ponds, wells). the impa ...19921600235
digestion of bacillus thuringiensis var. israelensis spores by larvae of aedes aegypti.the larvicidal activity of bacillus thuringiensis var. israelensis against mosquitoes and the blackfly is included in parasporal crystalline bodies which are produced during sporulation. following ingestion, the crystals are solubilized in the larval midgut and induce death within a short time; the spores germinate in the dead larvae and complete a growth cycle. the fate of the spores in surviving live larvae was elucidated by using a nonlarvicidal b. thuringiensis var. israelensis mutant. when ...19921607667
a novel bioassay system for evaluating the toxicity of bacillus thuringiensis israelensis against mosquito larvae.a bioassay system employing acutely toxic concentrations of a spore-crystal mixture of bacillus thuringiensis subspecies israelensis against fourth instar larvae of aedes aegypti (l.) is described. individual larvae are separately exposed to toxin in glass-lined miniature wells or scintillation vials. this method is free from the deaths due to predation among larvae. such larval deaths are commonly encountered in bioassay groups of 25 larvae as currently specified in the world health organizatio ...19921607671
phenol oxidase activity in hemolymph compartments of aedes aegypti during melanotic encapsulation reactions against microfilariae.monophenol oxidase (mpo) and diphenol oxidase (dpo) activity was assessed in hemocytes, cell-free plasma and complete hemolymph collected from aedes aegypti liverpool strain, intrathoracically inoculated with saline alone, immune activated by the inoculation of dirofilaria immitis microfilariae (mff), and uninoculated. enzyme activities between groups were compared using a radiometric hydroxylation assay (mpo) and a high pressure liquid chromatography with electrochemical detection assay (dpo). ...19921618354
results of a community-based aedes aegypti control program in merida, yucatan, mexico.this report describes the results of a community-based aedes aegypti control program in merida, yucatan, mexico. baseline surveys concerning knowledge, beliefs, and practices of 577 women and a larval survey of 616 house lots were carried out in october 1989. following development of a public health communication intervention from this data, the program was implemented in six communities. evaluation used an untreated control group design with pretest and two post-tests, one at completion and one ...19921621887
first reported outbreak of classical dengue fever at 1,700 meters above sea level in guerrero state, mexico, june 1988.an outbreak of classical dengue fever occurred from march to august 1988 in the city of taxco, guerrero state, mexico. taxco is at an elevation of 1,700 meters above sea level, and this study represents the highest altitude at which an outbreak of dengue has been documented. an investigation was conducted to obtain serologic confirmation of dengue infection, determine the extent of the outbreak, and identify risk factors for dengue illness. toxorhynchites cell lines were used for viral isolation ...19921621889
induced polypeptides associated with filarial worm refractoriness in aedes aegypti.brugia malayi and wuchereria bancrofti are mosquito-borne parasitic nematodes responsible for lymphatic filariasis in approximately 90 million people. the genetic control of the susceptibility of aedes aegypti mosquitoes to b. malayi was well defined 30 years ago, but no data have since been provided regarding the gene products responsible for susceptibility or refractoriness or both. we addressed this problem by assessing polypeptide synthesis in thoracic tissue, the developmental site of this ...19921631149
the diel pattern of entry to outdoor oviposition sites by female aedes aegypti (l.) (diptera: culicidae) that then laid eggs there: a preliminary study.experimental observations, made outdoors during the dry and wet seasons in trinidad, demonstrate that more than 70% of female aedes aegypti that are destined to oviposit within the next two hours enter ovitraps during the first and last two-hour periods in the day, a finding consistent with the diel pattern of oviposition recorded earlier at the same site. it is possible that proportionately more eggs are laid in the morning peak during the dry season than the wet season. fewer eggs are laid wit ...19921288434
[the campaign against aedes aegypti]. 19921296682
[observation on the effectiveness of repellent coating against mosquitoes].the present paper reports on the effectiveness of a repellent coating for the first time. the repellent coating was made by combining deta (n,n-diethyl-meta-toluamide) with slow-releasing reagent. with this coating employed in the laboratory against aedes aegypti, the rate of blood-sucking was 3.9-9.5% and the rate of protection was 88.9-95.3% for 1-720 days. during 1-77 days' field trial, the rpi (relative population index) was 0-8.98% in the day-time and 0.74-17.15 during the night-time agains ...19921307278
susceptibility of selected strains of aedes aegypti and aedes albopictus (diptera: culicidae) to chikungunya virus.the relative susceptibility of selected strains of aedes aegypti (l.) and aedes albopictus (skuse) fed on a viremic monkey to infection with chikungunya virus was determined. infection rates were consistently higher in 10 strains of ae. albopictus tested than in 7 strains of ae. aegypti tested, regardless of the geographic location from which the strains originated or the dose of virus ingested. similarly, virus dissemination rates were higher in the ae. albopictus strains compared with the ae. ...19921313111
the complete nucleotide sequence of cell fusing agent (cfa): homology between the nonstructural proteins encoded by cfa and the nonstructural proteins encoded by arthropod-borne flaviviruses.cell fusing agent (cfa) is an rna virus originally isolated from a line of aedes aegypti mosquito cells. although our characterization of the virus many years ago showed that it resembled the flaviviruses, there was no detectable serological cross-reaction with members of the genus flavivirus. furthermore, unlike the well-studied members of the genus flavivirus, cfa did not replicate in any of several vertebrate cell lines tested. we have now determined the nucleotide sequence of the cfa genome. ...19921322586
enzymes in saliva from four parasitic arthropods.enzyme assays and sds polyacrylamide gel electrophoresis were carried out on saliva and in some cases homogenates of salivary gland and gut from four parasitic arthropods (the cattle tick, boophilus microplus (canestrini); the mosquito, aedes aegypti (l.); non-parasitic adult and parasitic larval blowfly of sheep, lucilia cuprina (wiedemann); the buffalo fly, haematobia irritans exigua de meijere). saliva from all species showed large differences in the number and molecular weight of components, ...19921330086
isolation of getah virus from mosquitos collected on hainan island, china, and results of a serosurvey.an isolate of getah virus was obtained from culex mosquitos collected in mao'an village, baoting county, hainan province, china, in 1964. the virus (strain m-1) replicated in laboratory-bred aedes aegypti and cx. fatigans (= quinquefasciatus), and was transmitted by laboratory-bred ae. albopictus to healthy newborn albino mice. skeletal muscles of newborn albino mice experimentally infected with the virus showed degeneration, atrophy, necrosis, and inflammatory changes of muscle fibers. antibody ...19921338481
a new serotype of bacillus thuringiensis from colombia toxic to mosquito larvae.during a survey conducted in colombia a new isolate of bacillus thuringiensis that showed toxicity toward culex quinquefasciatus, cx. pipiens, aedes aegypti, and anopheles stephensi larvae was isolated. parasporal crystals were spherical in shape and showed a great degree of similarity with those produced by the reference strain of bacillus thuringiensis subsp. israelensis. supernatant fraction of the whole culture was not toxic, and heat-stable exotoxin production was negative. catalase, urease ...19921347310
[the efficacy of a combined preparation based on bacillus sphaericus and bac. thuringiensis h-14 against the larvae of blood-sucking mosquitoes].the optimal bactoculicide/sphaerolarvicide ratio in combination using against aedes aegypti larvae was established in laboratory by selection. the field trials of the selected bs/bt sample containing the ingredients in titer: bactoculicide-83, sphaerolarvicide-17 were conducted in the arid area of central asia. with 1 g/m2, complete mortality of culex, aedes and anopheles larvae was obtained with the residual larvicidal action of 15 days. it is recommended to carry out more large-scale trials of ...19921354836
comparison of bacillus thuringiensis subsp. israelensis cryiva and cryivb cloned toxins reveals synergism in vivo.when the gene for the mosquitocidal protein cryiva was expressed in two strains of bacillus thuringiensis (bt) cured of their resident delta-endotoxin genes, the protein accumulated as large inclusions. the inclusions produced in the bt subsp. kurstaki recipient strain were twice as soluble at alkaline ph as the inclusions produced in bt subsp. israelensis. solubilized protoxins were activated by treatment with mosquito gut extracts or trypsin for varying lengths of time and tested for in vitro ...19921355748
adulticidal effect of cyfluthrin against mosquitos of public health importance in malaysia.the efficacy of two formulations, wettable powder and emulsifiable concentrate, of cyfluthrin sprayed on plywood [10 mg (ai)/m2] was assessed against six species of mosquitos. the bioassay followed the who standard method, with some modification for the bioassay of insecticidal deposits on wall surfaces. the results indicated that these two formulations of cyfluthrin were effective against anopheles dirus and mansonia uniformis, moderately toxic to aedes aegypti and ae. albopictus in decreasing ...19921355928
infectivity and pathogenesis of iridescent virus type 22 in various insect hosts.this paper reports the results of a series of laboratory experiments to determine the infectivity and pathogenesis of iridescent virus type 22 (iv 22) for six species of mosquitoes, phlebotomine sand flies and triatomid bugs. following inoculation, iv 22 replicated in all of the species tested, without producing noticeable mortality within a 14 day observation period. examination of the infected insects by immunofluorescence demonstrated large amounts of viral antigen in many different organs. e ...19921355961
host range of clostridium bifermentans serovar. malaysia, a mosquitocidal anaerobic bacterium.clostridium bifermentans serovar. malaysia (c.b.m.) is toxic to mosquito larvae. in this study, we quantified its toxicity to the mosquitoes, aedes aegypti, ae. albopictus, ae. caspius, ae. detritus, anopheles stephensi, an. gambiae, culex pipiens and cx. quinquefasciatus. anopheles larvae are the most susceptible, followed by ae. detritus and ae. caspius, then culex and other aedes larvae. according to mosquito species, the lc50 varies from 7 x 10(3) to 1.3 x 10(6) cells/ml. three concentration ...19921357087
virus-dependent mortality in rift valley fever, eastern equine encephalomyelitis, and chikungunya virus-inoculated mosquito (diptera: culicidae) larvae.the effect of inoculation of mosquito larvae with rift valley fever (rvf) virus on survival to the adult stage was evaluated in aedes aegypti (l.), ae. fowleri (charmoy), ae. mcintoshi huang, ae. taeniorhynchus (wiedemann), ae. triseriatus (say), eretmapodites quinquevittatus theobald, anopheles albimanus wiedemann, and culex pipiens l. pupation rates were similar for rvf virus-inoculated and diluent-inoculated larvae of all mosquito species tested except cx. pipiens. however, with the exception ...19921357176
suppression of aedes aegypti by predatory toxorhynchites moctezuma in an island habitat.larval populations of the mosquito aedes aegypti were suppressed by predatory toxorhynchites moctezuma mosquito larvae released systematically in a village on union island (saint vincent and the grenadines) during march-december 1988. eggs and larvae of tx.moctezuma were transported from trinidad and introduced into all semi-permanent and permanent water-holding containers in the experimental village at clifton. the semi-isolated village of ashton served as control. base-line ae.aegypti indices ...19921358269
evaluation of the residual efficacy of permethrin-impregnated screens used against mosquitoes in marigat, baringo district, kenya.insecticide-impregnated screens and bednets are gradually finding wider use in malaria control programmes. the efficacy of these devices is dependent on the method of application, the acceptability by the people and effectiveness of the insecticide used. the present studies were carried out to determine the duration of the effectiveness of a permethrin-impregnated wall cloth (mbu cloth) used in the marigat area of baringo district, kenya in order to ensure its effective use. cotton cloth impregn ...19921363445
distribution, frequency, and diversity of bacillus thuringiensis in an animal feed mill.bacillus thuringiensis was isolated from 36 of 50 residue samples obtained from an animal feed mill (a stored-product environment). of 710 selected colonies having bacillus cereus-b. thuringiensis morphology isolated from the samples, 477 were classified as b. thuringiensis because of production of parasporal delta-endotoxin crystals. there was a diverse population of b. thuringiensis, as revealed by differentiation of the isolates into 36 subgroups by using (i) their spectra of toxicity to the ...199216348699
magnetic resonance studies of eukaryotic cells. iii. spin labeled fatty acids in the plasma membrane.xc sarcoma, vero and aedes aegypti plasma membranes have been studied in viable cells and in purified membrane of xc sarcoma cells by the spin label method. the temperature dependence of the order parameter of fatty acid spin labels is found to be linear in all three cells and membrane and shows no evidence of a lipid phase transition. the order parameter of the fatty acid labels substituted at the 5-position is shown to increase as a function of the cholesterol: phospholipid molar ratio in cell ...1992206284
use of genetic polymorphisms detected by the random-amplified polymorphic dna polymerase chain reaction (rapd-pcr) for differentiation and identification of aedes aegypti subspecies and populations.amplification of random regions of genomic dna using 10-base primers in the random-amplified polymorphic dna polymerase chain reaction (rapd-pcr) was used to differentiate and identify mosquito populations based on genetic variation. genomic dna was extracted from individual mosquitoes from 11 geographic populations of aedes aegypti and amplified in pcr reactions using single primers of arbitrary nucleotide sequence. discriminant analysis of the population frequencies of rapd fragments produced ...19921471745
reduced mosquito production in cemetery vases with copper liners.water-holding stone vases were sampled in 4 central florida cemeteries to compare the prevalence of mosquitoes in containers with and without metallic liners. overall, immature mosquitoes were found in more than 60% of the vases lacking liners and in more than 50% of the vases with aluminum liners. significantly fewer vases with copper liners were positive for mosquitoes. high mortality and a lack of development were observed in a field test involving the introduction of aedes aegypti larvae int ...19921361942
a simple method for cultivating freshwater copepods used in biological control of aedes aegypti.a simple method for indoor and outdoor cultivation of mesocyclops aspericornis, macrocyclops albidus and mesocyclops n. sp. copepods is presented. this method utilizes chilomonas sp., paramecium caudatum and fresh lettuce as food sources for copepod cultures. steps for initiating and maintaining copepod cultures are provided.19921474389
sequence and secondary structure comparisons of its rdna in mosquitoes (diptera: culicidae).sequences of the internal transcribed spacers (its1 and its2) of the mosquito aedes aegypti, and the its2 of six related species, a. simpsoni, a. albopictus, a. vexans, a. triseriatus, haemagogus mesodentatus, and psorophora ferox are reported. intraspecific variation in a. aegypti its1 is 1.07% among four clones from three individuals, and in the its2 is 1.17% among 15 clones from four individuals. in a. simpsoni, intraspecific its2 variation is 0.46% among 10 clones from a single individual. a ...19921364170
importance of ecology in aedes aegypti control.aedes aegypti is one of the world's most widely distributed mosquitos and is of considerable medical importance as a vector of dengue and yellow fever. not surprisingly therefore there has been more written on its biology than any other mosquito. the present paper summarizes ecological studies undertaken on this vector, including those on pre-adult mortalities, life-tables, adult dispersal and survival rates. in discussing surveillance techniques it is pointed out there are still no reliable met ...19921298074
food ingestion and digestive enzymes in larval aedes aegypti and ae. albopictus (diptera: culicidae).frequency of the labral brush movements of first, second, and fourth instars of aedes aegypti (l.) and aedes albopictus (skuse) was studied comparatively in the laboratory. a frequency of 197 strokes per min for the first and second instars was observed in the former species compared to 118 strokes per min in the latter species. a faster ingestion rate of algal cells also was observed in first and second instars of ae. aegypti (mean 57.5 cells per s) compared with first and second instars of ae. ...19921460635
variation in aedes aegypti mrna populations related to strain, sex, and development.translation products were used to monitor changes in gene expression between different strains and developmental stages of aedes aegypti. total rna was isolated from fourth stadium larvae, male and female pupae, and male and female adults collected at fixed time intervals following pupation and ecdysis. differences in rna populations were assessed by in vitro translation followed by one- and two-dimensional polyacrylamide gel electrophoresis and fluorography. variations in gene expression betwee ...19921449211
interspecific variation in desiccation survival time of aedes (stegomyia) mosquito eggs is correlated with habitat and egg size.survival times of eggs under three humidity conditions (42%, 68%, 88% rh) were investigated among aedes (stegomyia) mosquitoes from temperate and tropical zones (5 species and 20 geographical strains). this subgenus tends to occupy small aquatic sites as larvae, where desiccation resistance of eggs is necessary during habitat drought. interspecific comparison showed that the egg survival time was correlated with egg volume and dryness of source locality, and probably with habitat. aedes aegypti ...199228313521
beta-cyfluthrin, a synthetic pyrethroid for mosquito control.beta-cyfluthrin (oms 3051), a new synthetic pyrethroid and one of the stereoisomers of cyfluthrin, was studied for insecticidal activity against eight mosquito species. its larvicidal activity with lc50 values of 5.62 x 10(-5) and 1.19 x 10(-4) mg/l respectively for culex quinquefasciatus and aedes aegypti was comparable with that of deltamethrin. this pyrethroid was more effective against the larvae of armigeres subalbatus (lc50 - 7.76 x 10(-7)) and the adults of anopheles culicifacies lt50 - 2 ...19921359650
reproductive and metabolic differences between aedes aegypti and ae. albopictus (diptera: culicidae).aedes albopictus (skuse) females were more likely to develop eggs after they ingested small blood meals than were aedes aegypti (l.) when both species were maintained under the same suboptimal adult nutritional regimen. the longevity of adult female ae. albopictus under conditions of starvation was also significantly greater than that of ae. aegypti. analyses of total body proteins, lipids, and glycogen indicated that the increased reproductive efficiency of ae. albopictus may be a result of its ...19921625295
aedes aegypti (diptera: culicidae) vision: response to stimuli from the optical environment.although aedes aegypti (l.) are capable of some wavelength discrimination, behavioral experiments demonstrated that color was not a stimulus to which they responded. optical stimuli that did affect ae. aegypti behavior were luminous reflectance, vertical contrast, and movement. stationary objects of low reflectance and solid color were the most attractive to males and females.19921625292
digestibility by and pathogenicity of the protozoa tetrahymena pyriformis to larvae of aedes aegypti. 19921607672
invasion of cemeteries in florida by aedes albopictus.aedes albopictus has been found in 53 of the 67 florida counties. the initial discoveries in 11 of these counties were made in cemeteries. at several locations, ae. albopictus became well-established in cemeteries before appearing in nearby accumulations of waste tires. the recycling of plastic floral baskets may be aiding the spread of ae. albopictus. mosquitoes were commonly found in all types of flower-holding containers in cemeteries, except bronze vases. in the laboratory, most aedes aegypt ...19921583479
in vitro assay for the biosynthesis and metabolism of juvenile hormone by exposed corpora allata of aedes aegypti (diptera: culicidae).a technique has been developed to study jh iii biosynthesis in vitro by removing the head and thorax of aedes aegypti (l.) and exposing the corpora allata (ca). exposed ca were incubated with [12-3h]methyl farnesoate, and the newly synthesized jh iii and jh iii metabolites were followed using c18 reversed-phase, high-performance liquid chromatography (hplc) and preparative gas chromatography. the rate of synthesis of [12-3h]jh iii by exposed ca from newly emerged adult ae. aegypti was 23 fmol/ca ...19921495050
aedes aegypti (diptera: culicidae) vision: spectral sensitivity and other perceptual parameters of the female eye.from measurements of the mean ommatidial diameter (17.2 microns) and mean interommatidial angle (6.2 degrees) of the eye of female ae. aegypti (l.), a minimum resolvable angle of 12.3 degrees and an eye parameter of 1.6 microns were calculated. the eye had relatively poor acuity but high overall sensitivity to light. electroretinographs revealed spectral sensitivity ranging from ultraviolet (323 nm) to orange-red (621 nm), with sensitivity peaks in the ultraviolet (lambda max323-345 nm) and gree ...19921495042
laboratory colonization and maintenance of toxorhynchites moctezuma.a colony of toxorhynchites moctezuma was established at the caribbean epidemiology centre in trinidad in 1984. toxorhynchites moctezuma was maintained in cages with high humidities. eggs were deposited most frequently in a cut bicycle tire containing water. a minimum of 42 h was required for hatching, but 94% hatched between 43 and 51 h. aedes aegypti larvae were supplied as prey. larval development times varied with the quantity of prey offered, but when fed ad lib, peak developmental time was ...19921349915
selection of the yellow fever mosquito aedes aegypti for cheap and easy maintenance without bloodmeals. 19921600234
infectivity and pathogenicity of ascogregarina culicis (eugregarinida: lecudinidae) to aedes aegypti (diptera: culicidae).the susceptibility of three strains of aedes aegypti (l.) to four strains of ascogregarina culicis were studied under laboratory conditions. the parasite was found to be pathogenic to the mosquito; although pathogenicity varied among geographical strains of a. culicis and susceptibility varied among geographical strains of ae. aegypti. in addition to affecting the survival of the mosquito larvae, infection with the parasite was found to shorten larval development time. selected strains of a. cul ...19921552514
the mosquitocidal activity of conidia of tolypocladium tundrense and tolypocladium terricola.tolypocladium tundrense and t. terricola uv-irradiated conidia exhibited acute toxicity to aedes aegypti larvae in concentrations of 5 x 10(5) and 5 x 10(6) ml-1, respectively. substances deposited on the surface of conidia following sporulation appear to be responsible for rapid intoxication and killing of mosquito larvae. treatment of larvae with dried methanolic extract of the spores also induced a high level of mortality accompanied by the same histological changes, including vacuolization a ...19921505208
generation of superoxide anion radical by alpha-terthienyl in the anal gills of mosquito larvae aedes aegypti: a new aspect in alpha-terthienyl phototoxicity.the present study documents that the secondary plant metabolites, especially alpha-terthienyl, exert phototoxic action through inhibition of certain enzymes and generation of singlet oxygen. some of the reports have emerged exhibiting involvement of free radical generation in vitro by alpha-terthienyl. we provide evidence for the generation of a free radical viz., superoxide anion radical, by alpha-terthienyl employing spin-trapping techniques, probably due to the extension of the latter reactio ...19921318115
evaluation of emd vaporizers and bioallethrin vaporizing mats against mosquito vectors.different electric-mat-device (emd) vaporizers and bioallethrin impregnated mats were evaluated for the knockdown and mortality in the adults of culex quinquefasciatus, anopheles stephensi and aedes aegypti. percentage knockdown of 80-100 was recorded throughout the test period of 11 hours. however, the percentage mortality observed was not appreciable in all types of emd vaporizers. device "a" was found to be effective only against ae. aegypti. devices "c" and "d" showed moderate effects agains ...19921488702
synthesis of the orally macrofilaricidal and stable glycerolipidic prodrug of melphalan, 1,3-dipalmitoyl-2-(4'(bis(2''-chloroethyl)amino)phenylalaninoyl)gl ycerol.a new strategy is presented to develop macrofilaricidal compounds orally administered and able to concentrate in the lymphatic system. a diglyceride derivative of melphalan, 1,3-dipalmitoyl-2-(4'(bis(2''-chloroethyl)amino)phenylalaninoyl)gl y cerol, was synthesized. the esterification of melphalan by 1,3-dipalmitin allowed chemical stabilization of the alkylating agent in aqueous dispersion. no degradation of this prodrug was observed after a 3-month storage of an aqueous dispersion at 4 degrees ...19921445485
unique electrophysiological effects of dinitrophenol in malpighian tubules.in the course of electrophysiological studies of malpighian tubules of the mosquito aedes aegypti, we have found unusual effects of 2,4-dinitrophenol (dnp) that offer new insights into the electrogenic and conductive properties of the tubule. dnp (10(-4)m) depolarized the basolateral membrane voltage from -58.0 to -3.3 mv, and it depolarized the apical membrane voltage from 110.6 to 8.9 mv. in parallel the transepithelial electrical resistance increased from 11.4 to 16.8 k omega.cm, and the frac ...19921415649
differences in male mating response and female flight sounds in aedes aegypti and ae. albopictus (diptera: culicidae).aedes aegypti (l.) and ae. albopictus (skuse) showed similar rates and timing of insemination in the laboratory. laboratory attempts at interspecific mating were unsuccessful. because ae. aegypti males are known to locate females by flight sounds, male response to sound was compared in the two species. ae. aegypti males responded to female flight sounds with stereotypical orientation and mating behavior, whereas ae. albopictus males seldom responded. recorded flight sounds of females were sample ...19921404257
aedes albopictus and other mosquitoes imported in tires into durban, south africa.the results of surveillance for immature stages of mosquitoes in samples of wet tires in 5 consignments imported into durban from japan is reported. three of these consignments contained tires with immatures of aedes aegypti, ae. albopictus and uranotaenia n. novobscura. the proportions of all the wet tires calculated to contain mosquitoes were 189/1,488 (13%), 20/813 (2.5%) and 13/1,032 (1.3%) which emphasizes the need for effective mosquito control measures to prevent ae. albopictus escaping f ...19921402872
sublethal effects of larval methoprene exposure on adult mosquito longevity.larvae of aedes aegypti were exposed to sublethal concentrations of the insect growth regulator, methoprene, and the glycogen content of pupae and surviving adults was compared and effects on adult longevity determined. the glycogen reserves in both male and female ae. aegypti pupae were significantly reduced as a result of methoprene exposure. the longevity of adult females was also significantly reduced, but exposure affected neither the longevity nor the glycogen content of adult males. adult ...19921402867
comparative efficacy of aphid extracts and some juvenoids against the development of mosquitoes.comparative efficacy of natural juvenile hormones extracted from aphis craccivora and a. gosypii and 5 juvenoids, i.e., methoprene, neporex, oms 3007, oms 3019 and dpe-28 on the development of aedes aegypti, anopheles stephensi and culex quinquefasciatus have been evaluated. oms 3007, oms 3019, dpe-28 and neporex show species specific related activities, while methoprene and aphid extracts do not show such activity against these mosquito species. treatment of mosquito eggs with an ec50 dose of t ...19921402861
relationship of hemolymph phenol oxidase and mosquito age in aedes aegypti.monophenol oxidase (mpo) and diphenol oxidase (dpo) activity in hemocytes and cell-free plasma perfused from 7-, 14-, 21-, and 28-day-old aedes aegypti mosquitoes were compared. a progressive decrease of enzyme activity was detected as mosquito age increased, and this decrease was significant in both hemocytes and cell-free plasma when mosquitoes were 28 days old as compared with that found in 7-day-old mosquitoes. there was no significant difference in total hemolymph protein as mosquito age in ...19921401989
[susceptibility of thirty-one species of mosquitoes to romanomermis yunanensis].a new species of mermithidae was found parasitizing the larvae of culex tritaeniorhynchus and culex fatigans in henan, china and then named romanomermis yunanensis. thirty-one species of mosquitoes involving six genera have been tested for susceptibility to r. yunanensis and culicinae mosquito have consistently been highly susceptible. at a 1:5 ratio of mosquito larvae to nematode juveniles, the parasitisms of aedes aegypti, ae. albopictus, culex fatigans, culex tritaeniorhynchus, anopheles sine ...19921363920
cannibalism and carnivory in toxorhynchites splendens (diptera: culicidae).laboratory reared larvae of toxorhynchites splendens, which were previously starved for 24 hours, cannibalized eggs of their own kind or preyed upon the eggs of other species (aedes aegypti and anopheles stephensi) present on the surface of water in small containers. second and third instars consumed eggs faster than first and fourth instars. the first instar consumed larvae of its own kind faster than the other instars, in the absence of other prey. however, when prey larvae were provided, ther ...19921362626
diel sugar-feeding and host-seeking rhythms in mosquitoes (diptera: culicidae) under laboratory conditions.mosquito sugar-feeding and host-seeking rhythms were recorded in the laboratory using remote sensors. in all five species studied, the two rhythms were nearly synchronous. in anopheles quadrimaculatus say, culex quinquefasciatus say, and aedes triseriatus (say), host-seeking activity increased slightly earlier than sugar feeding in the evening. the evening sugar-feeding period was longer than the evening host-seeking period in aedes aegypti (l.), but the two periods were of similar duration in a ...19921357175
genetic and molecular evidence for a trans-acting regulatory locus controlling glutathione s-transferase-2 expression in aedes aegypti.the amount of glutathione s-transferase-2 (gst-2) protein and enzyme activity in a mutant strain (strain gg) of the yellow fever mosquito (aedes aegypti) is approximately 25-fold higher than in the wild-type (++) strain. the mode of inheritance of the gg phenotype was studied in f1 and backcross progeny using gst enzyme assays, isozyme-specific antisera, and northern blot analysis. enzyme assay of parental and f1 progeny showed that the ++ phenotype was dominant to the gg phenotype. this was tru ...19921508145
biochemical and electrophoretical studies of the effect of some chemosterilants on theenzymes of aedes aegypti (l.) larvae.in vivo effect of standard and prospective chemosterilants on some enzymes is reported. colorimetric and histochemical findings indicate that alkaline phosphatase is inhibited by shikonin, hempa, tepa and shikonin angelate. cholinesterase is inhibited by all the compounds while diphenol oxidase is not inhibited. these findings have been discussed in the light of the earlier observations.199216414718
effect of the repellent deet on the antennal chemoreceptors for oviposition in aedes aegypti (diptera: culicidae).the insect repellent, n,n-diethyl-m-toluamide (deet), interferes with detection of the host attractant, lactic acid (la), by la-excited neurons on the antennae of female aedes aegypti (l.) mosquitoes. in the current laboratory behavioral experiment, the attraction of gravid female mosquitoes to a source of ethyl propionate, an oviposition attractant, was reduced by a factor of two when deet was present. similarly, when deet was presented together with ethyl propionate in electrophysiological exp ...19921495074
in vivo and in vitro biosynthesis and metabolism of methyl farnesoate, juvenile hormone iii, and juvenile hormone iii acid in the mosquito aedes aegypti.biosynthesis and metabolism of juvenile hormone (jh) iii in vivo and in vitro were studied in female aedes aegypti (l.). [12-3h]methyl farnesoate was used to follow the synthesis and [12-3h]-(10r)-jh iii to study metabolism. the rate of biosynthesis of [12-3h]jh iii in vivo after adult eclosion increased from 9 fmol/h per female at 1 h to 22 fmol/h per female at day 6. the rate of biosynthesis by exposed corpora allata (ca) in vitro was 23 fmol/h per ca during the 1st d after adult eclosion, the ...19921495071
mosquito repellent activity of oils from vitex negundo linn. leaves.oil obtained from stream distillate of v. negundo leaves was fractionated by column chromatography. mosquito repellence activity, as evaluated against aedes aegypti was mainly confined to the most polar fractions. the protection period against mosquito bites by polar fractions ranged between 1-3 h. however, the mean protection period values of these fractions did not show significant increase in the subsequent subfractions.19921398810
effects of toxorhynchites moctezuma larval predation on aedes aegypti populations: experimental evaluation.predatory larvae of the mosquito toxorhynchites moctezuma were used experimentally to control a standing crop of larvae of the dengue vector mosquito ae.aegypti. each week, fifty ae.aegypti first instar larvae were introduced to each of five water-filled drums (220 litres) of the type commonly used for domestic water storage in caribbean dwellings. at the beginning of the fourth week, a certain number (0, 1, 2, 5 or 10) of first instar tx.moctezuma larvae were introduced to each drum and the dai ...19921358268
expression of cryiva and cryivb genes, independently or in combination, in a crystal-negative strain of bacillus thuringiensis subsp. israelensis.the cryiva and cryivb genes, encoding the 125- and 135-kda proteins, respectively, of bacillus thuringiensis subsp. israelensis, were cloned either alone or together into a shuttle vector and expressed in a nontoxic strain of b. thuringiensis subsp. israelensis. the cryivb protein was produced at a high level during sporulation and accumulated as inclusions; in contrast, the cryiva polypeptide did not form such structures unless it was cloned on a higher-copy-number plasmid. transcriptional fusi ...199316349095
extracts of mosquito salivary gland inhibit tumour necrosis factor alpha release from mast cells.extracts of salivary glands of the yellow fever mosquito aedes aegypti inhibit tumour cell-stimulated release of tumour necrosis factor alpha (tnf alpha) from rat mast cells, but do not inhibit antigen-induced histamine secretion. this inhibitory activity for tnf alpha is found in salivary glands of female but not in male mosquitoes. this inhibition is not mediated by bacterial contamination (lps), by calcitonin gene related peptide (cgrp), nerve growth factor (ngf), epidermal growth factor (egf ...19937679483
ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral rna from clinical specimens and mosquitoes by reverse transcriptase-polymerase chain reaction.a rapid, simple and efficient single-tube procedure is described for the isolation of dengue virus rna from small amount of serum (10 microliters) followed by a reverse transcriptase-polymerase chain reaction (rt-pcr). recovery of rna is based on the lysing and nuclease-inactivating properties of guanidinium thiocyanate in the presence of silica. the silica rt-pcr can be completed within 5 hours starting from rna extraction to agarose gel electrophoresis. all of the 63 dengue-3 culture-positive ...19937689635
[dengue epidemic in new caledonia (1989). environmental factors and prevention].an extensive dengue epidemic was reported in new caledonia en 1989. environmental factors (temperature and rainfalls) directly controlled the mosquitos population at the end of 1988. the introduction of a new dengue virus, transmitted by aedes aegypti, was responsible for an epidemic that occurred in january-april 1989 and lasted until july 1989. the estimated number of cases was 25,000 and the attack rate was close to 120 per thousand inhabitants for a six month-period. the epidemic involved ma ...19937819798
[current situation of yellow fever in latin america].yellow fever endemicity is currently stabilized in south america: an average of 115 cases has officially been notified each year since 25 years (between 12 and 304 annual cases). these figures are underevaluated but no epidemic has been observed since 1983. only sporadic or limited grouped cases have been reported. all declared cases correspond to people who have been infected within sylvatic areas, mainly adult forestmen. within the last years, the majority of cases have occurred in peru and bo ...19937819801
mosquito host range and specificity of edhazardia aedis (microspora: culicosporidae).edhazardia aedis was transmitted horizontally to its natural host, aedes aegypti, and to 6 alternate hosts: ae. albopictus, ae. triseriatus, ae. taeniorhynchus, anopheles quadrimaculatus, orthopodomyia signifera and toxorhynchites rutilus rutilus. the microsporidium produced both binucleate and uninucleate spores in all susceptible hosts. transovarial transmission, however, was only successful in ae. aegypti. therefore, while e. aedis can infect a variety of mosquito species from diverse genera, ...19937902414
evaluation of organophosphorus and synthetic pyrethroid insecticides against six vector mosquitoe species.three organophosphorus compounds- malathion, folithion and temephos- and two synthetic pyrethroids- alphamethrin and deltamethrin- were used for monitoring the susceptibility status of larvae and adults of six vector mosquito species: culex quinquefasciatus (filariasis) and aedes albopictus (dengue) (both laboratory and field strains); laboratory strains of aedes aegypti (dengue), anopheles stephensi and anopheles culicifacies (malaria), and culex tritaeniorhynchus (japanese encephalitis) in ind ...19937997809
cytotoxicity and adp-ribosylating activity of the mosquitocidal toxin from bacillus sphaericus ssii-1: possible roles of the 27- and 70-kilodalton peptides.clones expressing regions of the 100-kda bacillus sphaericus ssii-1 mosquitocidal toxin (mtx) as fusion proteins with glutathione s-transferase were constructed, and the toxin-derived peptides were purified. the in vitro adp-ribosylation activities of these peptides and their effects on larvae and cells in culture were studied. mtx25 (amino acids 30 to 493) was found to adp-ribosylate two proteins with molecular masses of 38 and 42 kda, respectively, in culex quinquefasciatus (g7) cell extracts, ...19938096838
effect of temperature on toxicity of two bioinsecticides spherix (bacillus sphaericus) and bactoculicide (bacillus thuringiensis) against larvae of four vector mosquitoes.two bioinsecticide preparations, viz. spherix (bacillus sphaericus) and bactoculicide (bacillus thuringiensis h-14), were tested in the laboratory against larvae of anopheles culicifacies, anopheles stephensi, culex quinquefasciatus and aedes aegypti at different temperatures. the lcs50 of spherix against iii instar larvae of these species at 27 +/- 2 degrees c were 2.0, 0.19, 0.05 and > 40 mg/litre, respectively and those of bactoculicide were 0.32, 0.16, 0.06 and 0.03 mg/litre, respectively. t ...19938100540
linkage map for aedes aegypti using restriction fragment length polymorphisms.we report construction of a genetic linkage map for the mosquito, aedes aegypti, based on restriction fragment length polymorphisms (rflps). the map consists of 50 dna markers that identify 53 loci covering 134 map units across three linkage groups. determination of linkage associations between rflp markers and several mutant marker loci allowed for partial integration of the rflp markers with an existing classical genetic linkage map for a. aegypti. the rflp markers include 42 random cdna clone ...19938101854
variation of larval susceptibility to lagenidium giganteum in three mosquito species.a significantly greater number of lagenidium giganteum zoospores were found encysting on the dorsal thoracic surface of anopheles gambiae larvae than on the larvae of aedes aegypti and culex pipiens. however, germ tube penetration in the cuticle of a. gambiae provoked an intense and diffuse melanization which encapsulated the fungus and protected 56% from death. although a small number of zoospores attach to and penetrate the cuticular surface of a. aegypti and c. pipiens approximately 99% of bo ...19938104999
intracellular development of subperiodic brugia malayi influenced by mosquito thoracic muscle cells.the in vitro development of 1-day-old intracellularly lodged larvae of brugia malayi cultured in infected excised thoraces of selected susceptible and refractory strains of aedes aegypti and anopheles quadrimaculatus was compared with larvae reared in vivo. in susceptible mosquitoes, both in vitro and in vivo, larvae developed normally and abnormally. in refractory mosquitoes this pattern of both normal and abnormal development was also observed, except that comparatively fewer larvae developed ...19938105000
dengue in the south-eastern region of brazil: historical analysis and epidemiology.the aim of the study is an historical analysis of the work undertaken by the public health organizations dedicated to the combat of the aedes aegypti, as well as an epidemiological study of persons with unexplained fever, with a view to evaluating the occurrence of dengue within the population. the mac-elisa, gac-elisa, hemagglutination inhibition, isolation and typage tests were used. organophosphate intoxication in agricultural workers was also assessed by measuring concentrations of seric cho ...19938115829
[comparative study of larval and ovitrap efficacy for surveillance of dengue and yellow fever vectors].a comparative study of the efficiency of ovitraps and larval-traps was undertaken with a view to improving the entomological survey of vectors of dengue and yellow fever-aedes aegypti and aedes albopictus-in s. paulo state, brazil. the region studied is infected only by aedes albopictus, a species that keeps to wild habitats but colonizes artificial breeding grounds as well. the first part of the study was located in a periurban area of tremembé county were 3 hollon trees, 23 ovitraps and 5 larv ...19938209154
[aedes (stegomyia) aegypti l. and associated culicidae fauna in a urban area of southern brazil].some aspects of the ecology of eleven species of culicidae that were found breeding in recipients in an urban area of southern brazil are presented. a great variety of recipients were listed as efficient breeding sites. apparently aedes aegypti has been recently introduced into the region and was limited to two areas of the city. culex quinquefasciatus, cx. coronator, ae. aegypti, ae. fluviatilis e limatus durhamii were the predominant species.19938209165
effect of ivermectin on two filaria-vector pairs. brugia malayi-aedes aegypti; litomosoides sigmodontis-bdellonyssus bacoti.the effect of ivermectin was studied on two filaria-vector pairs, brugia malayi-aedes aegypti and litomosoides sigmodontis-bdellonyssus bacoti. the rodent hosts, respectively mastomys coucha and meriones unguiculatus, were treated with ivermectin doses of 0.05 mg/kg, or 0.2 mg/kg or 2 mg/kg. batches of vectors were fed on rodents, infected or not, treated or not, from h7 to d43 post-ivermectin. vector survival was observed and dissections were performed to study the filarial development. it appe ...19938215116
effect of a community-based aedes aegypti control programme on mosquito larval production sites in el progreso, honduras.this paper describes the results of a trial to assess the efficacy in reducing the density of larval production sites of utilizing community involvement in the planning and implementation of an aedes aegypti control programme, in a dengue-endemic city in honduras. in addition to a substantial increase in knowledge about dengue transmission and prevention, a relative reduction in ae. aegypti larval infestation indices was found in those city neighbourhoods where community involvement was utilized ...19938236387
insect blood meal studies using radiosodium 24na and 22na.rabbits were infused with 3.7 gbq (100 mci) of [24na]na ion in a 100-ml sodium carbonate solution. beta particles were detected using a tennelec counting system; background counts were 1.6 +/- 1 counts per minute (cpm). counts for one nanoliter of blood ranged from 22 to 30 cpm. blood volumes on the mouthparts of tabanids following a 15-sec interrupted feeding were estimated to be 12.5 nl for tabanus fuscicostatus, 10.8 nl for t. nigrovittatus and 6.12 nl for chrysops fuliginosus. estimates of t ...19938245935
oviposition response of aedes aegypti mosquitoes to different concentrations of hay infusion in trinidad, west indies.ovitraps containing various concentrations of hay infusion and tap water were exposed weekly in the field for 15 wk to determine the oviposition patterns of aedes aegypti. the results showed 10, 20, 60 and 80% hay infusions each attracted similar numbers of ae. aegypti eggs oviposited and egg occurrences. no repellent effect was observed. in another field study, significantly more eggs and egg occurrences were collected from 25 and 50% hay infusions and tap water. the differences in these result ...19938245947
blood-feeding patterns of aedes aegypti (diptera: culicidae) collected in a rural thai village.aedes aegypti (l.) were collected by aspiration once each week from in and around houses in a rural village in chachoengsao province. thailand, during may 1990 to june 1991. of the 1,230 specimens analyzed with a sandwich elisa, 73% reacted to one or more of the seven hosts which we tested. eighty-eight percent (789/896) of all detectable meals were identified as being from a single host (human). patent multiple meals (double and triple), of which one was always human, were detected in 7% (66/89 ...19938254642
laboratory demonstration of oviposition by aedes aegypti (diptera: culicidae) in covered water jars.in thailand, ceramic jars (135-200 liters) covered by aluminum lids commonly are used to store water for household use. in laboratory cages, gravid female aedes aegypti (l.) were able to enter and oviposit in a covered water jar. although the aluminum lid was not a complete barrier to gravid females, it reduced the number of eggs oviposited by 77%. a vertically oriented foam rubber ring which was compressed between the lip of the jar and the flange of the lid effectively sealed the jar against o ...19938254646
the gonotrophic status and diel pattern of entry to outdoor oviposition sites of female aedes aegypti (l.) (diptera: culicidae).experimental observations, made outdoors during the dry and wet seasons in trinidad, confirm earlier findings regarding the diel pattern of entry to ovitraps and oviposition there and the taking of more than one bloodmeal during a single gonotrophic cycle. for 6-h but not for 6-h exposure periods, significantly more eggs were laid in the morning peak during the dry season than during the wet season. at the study site females were encountered singly, and only in ovitraps that contained eggs. with ...19938257237
seasonal abundance of dengue vectors in manila, republic of the philippines.the dengue vectors aedes aegypti and ae. albopictus were surveyed within the city of manila from august 1985 to july 1987. survey techniques included ovitraps, indoor resting collections, fay traps, and larval surveys. aedes aegypti was the principal container breeder within the residential areas, while ae. albopictus predominated in cemeteries. the ovitrap was very effective in detecting the presence of ae. aegypti, but not in measuring population changes during the year. indoor resting and fay ...19938266245
codon usage patterns in chromosomal and retrotransposon genes of the mosquito anopheles gambiae.codon usage was compiled for fourteen chromosomal genes and four retrotransposons from the mosquito anopheles gambiae. variation exists among chromosomal genes in the degree of bias. the genes showing the highest bias are probably most highly expressed. in these genes, the base composition at the third codon position is much richer in g + c than is the overall coding sequence. thus, codon usage is biased toward g- or c-ending codons. codon usage in each retrotransposon is quite different, not on ...19938269095
dynamic life table model for aedes aegypti (diptera: culicidae): analysis of the literature and model development.the container-inhabiting mosquito simulation model (cimsim) is a weather-driven, dynamic life table simulation model of aedes aegypti (l.). it is designed to provide a framework for related models of similar mosquitoes which inhibit artificial and natural containers. cimsim is an attempt to provide a mechanistic, comprehensive, and dynamic accounting of the multitude of relationships known to play a role in the life history of these mosquitoes. development rates of eggs, larvae, pupae, and the g ...19938271242
dynamic life table model for aedes aegypti (diptera: culicidae): simulation results and validation.the container-inhabiting mosquito simulation model (cimsim) is a weather-driven, dynamic life table simulation model of aedes aegypti (l.) and similar nondiapausing aedes mosquitoes that inhabit artificial and natural containers. this paper presents a validation of cimsim simulating ae. aegypti using several independent series of data that were not used in model development. validation data sets include laboratory work designed to elucidate the role of diet on fecundity and rates of larval devel ...19938271243
[outbreak of classical fever of dengue caused by serotype 2 in araguaiana, tocantins, brazil].we report the first outbreak of dengue fever caused by dengue 2 (den 2) in araguaina, tocantins state. four hundred people of 74 families, living at s. joão, araguaina sul and neblina districts were questioned and then bled, in order to obtain sera to test for anti-dengue antibodies. if a person was sick, a small quantity of blood was collected for virus isolation. the main clinical picture of disease was characterized by fever, headache, myalgias, arthralgias and skin rash. were obtained 1,105 ...19938284598
role of the cryivd polypeptide in the overall toxicity of bacillus thuringiensis subsp. israelensis.the gene encoding the cryivd protein of b. thuringiensis subsp. israelensis crystals was disrupted by in vivo recombination. the toxicity of the cryivd protein-free inclusions was similar to that of the wild-type crystals on anopheles stephensi larvae but was half the wild-type toxicity on culex pipiens and aedes aegypti larvae.19938285695
on the transmissibility of eperythrozoon suis by stomoxys calcitrans and aedes aegypti.the stable fly, stomoxys calcitrans (linnaeus), and the yellow fever mosquito, aedes aegypti (linnaeus), were utilized to determine their capability to transmit eperythrozoon suis splitter between swine. three groups of each insect in each trial were allowed to feed on a pig previously infected with e. suis and then transferred to susceptible splenectomized pigs. as a control, one group of each insect was fed on a non-infected pig and then transferred to a susceptible pig. stable flies were tran ...19938291187
stable transformation of mosquito cell lines using a hsp70::neo fusion gene.mosquito cell culture transfection will allow the advancement of genetic studies of these important disease-transmitting insects. towards this end, we report the generation of stably transformed aedes aegypti mos20 cells using a plasmid construct containing the tn5 neo gene, the drosophila melanogaster hsp70 promoter, an sv40 intron and poly adenylation sequence, and a pbr 322 backbone. the apparent frequency of transfection, as measured by transient resistance of cell colonies to geneticin (g41 ...19938293995
[the effect of water temperature on the action of bacterial insecticides against mosquito larvae].the ld50 logarithm of bacillus thuringiensis and b. sphaericus toxins effects on the larvae of aedes aegypti and culex pipiens is in inverse correlation with the water temperature. the influence of the temperature on the toxins effect on c. pipiens larvae is higher than that for a. aegypti larvae. similarly, b. sphaericus toxin effect depends on the water temperature to a greater measure than that of b. thuringiensis toxin. the authors predict a high efficacy of b. sphaericus-based insecticides ...19938336659
heterogeneity in patterns of malarial oocyst infections in the mosquito vector.oocyst prevalence and intensity have been recorded in 349 laboratory infections of anopheles stephensi with plasmodium berghei. intensity and prevalence of infection are shown to be predictably related. the structure and heterogeneity in the infections has been analysed with the objective of describing the biological mechanisms by which the observed negative binomial oocyst distributions are generated. the analysis has revealed that the most likely processes lie within the population dynamic eve ...19938341579
genetic maps of the sheep blowfly lucilia cuprina: linkage-group correlations with other dipteran genera.linkage data and revised genetic maps for 72 autosomal loci in lucilia cuprina are presented. comparison of the linkage relationships of biochemically and morphologically similar mutations in ceratitis capitata, drosophila melanogaster, and musca domestica supports the hypothesis that the major linkage elements have survived relatively intact during evolution of the higher diptera. the relationship of the linkage groups of the mosquito aedes aegypti to these species is less clear.19938349126
unreliable supply of potable water and elevated aedes aegypti larval indices: a causal relationship?we investigated the hypothesis that a deficient supply of piped water was causing a high prevalence of water storage containers, which in turn, become important aquatic habitats of aedes aegypti in a small town in venezuela. the house (71.2%) and breteau indices (229) were considerably elevated. prevalent positive containers were: metal drums, small disposable containers (bottles, tins, etc.), tires, house plants (flowers in vases and plants in pots with earth) and tanks. most people reported fr ...19938350076
identification of blood meals in aedes aegypti by antibody sandwich enzyme-linked immunosorbent assay.a comparative study of 3 elisa protocols was performed for host blood meal identification in aedes aegypti. in the sandwich-b elisa, which used a combination of heavy and heavy+light chain conjugates, specificity was improved to such a degree that conjugates no longer required cross-adsorption with heterologous sera. using the sandwich-b assay, human blood meals in laboratory reared ae. aegypti could be detected longer after feeding (100% at 32 h and 80% at 42 h) than with a direct assay (100% a ...19938350077
[a study on transmission of dengue virus by culex fatigans].patients with acute dengue fever within 2 days of onset were simultaneously bitten by culex fatigans, aedes aegypti and aedes albopictus, which were fed and incubated in the laboratory. isolation and quantitative assay of dengue virus and transmission experiment were conducted on specimens collected from 3 kinds of mosquitoes at different time. the result showed a positive rate of 5.13% (2/39) and an average viral titer of 2.41 logtcid50, for culex fatigans, which indicates that culex fatigans m ...19938353818
three simple devices for preventing development of aedes aegypti larvae in water jars.we developed three types of covers that are easily fabricated from plastic screen locally manufactured in thailand. the covers were designed to permit normal use of water jars for drinking, utility water uses, and water storage. tests of the covers in the laboratory and field demonstrated that they could completely prevent successful development of aedes aegypti (l.) in water jars. initial experience in a village setting demonstrated that these devices were readily manufactured and used in a com ...19938357077
temperature-induced refractoriness of aedes aegypti mosquitoes to infection with the filaria brugia pahangi. 19938369568
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