Publications

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clinical and epidemiological teaching of dengue through simulated practice.to describe the experience of clinical teaching on dengue and the practice of epidemiological surveillance using problematization methodology.201829412306
[eradication of aedes aegypti: new perspectives on international health cooperation in the americas]. 201829412266
context-dependent interactive effects of non-lethal predation on larvae impact adult longevity and body composition.predation impacts development, behavior and morphology of prey species thereby shaping their abundances, distribution and community structure. non-lethal threat of predation, specifically, can have a strong influence on prey lifehistory characteristics. while investigations often focus on the impact of predation threat on prey in isolation, tests of its interactive effects with food availability and resource competition on prey survival and fitness can improve understanding of costs, benefits an ...201829401513
molecular identification, transcript expression, and functional deorphanization of the adipokinetic hormone/corazonin-related peptide receptor in the disease vector, aedes aegypti.the recently discovered adipokinetic hormone/corazonin-related peptide (acp) is an insect neuropeptide structurally intermediate between corazonin (crz) and adipokinetic (akh) hormones, which all demonstrate homology to the vertebrate gonadotropin-releasing hormone (gnrh). to date, the function of the acp signaling system remains unclear. in the present study, we molecularly identified the complete open reading frame encoding the aedes aegypti acp receptor (acpr), which spans nine exons and unde ...201829391531
[the recent epidemic spread of zika virus disease].zika virus (zikv) is one of the members of the spondweni serocomplex within the genus flavivirus of the family flaviviridae. the virus was first isolated from a serum specimen from a sentinel non-human primate in the zika forest of uganda in 1947. zikv is transmitted by aedes aegypti and a. albopictus in an urban cycle and maintained in a sylvatic cycle between aedes mosquitoes and monkeys in africa and asia. initially, the virus was thought to cause only mild and nonspecific clinical symptoms i ...201831105130
increased adult aedes aegypti and culex quinquefasciatus (diptera: culicidae) abundance in a dengue transmission hotspot, compared to a coldspot, within kaohsiung city, taiwan.the assumption that vector abundance differences might drive spatial and temporal heterogeneities in vector-borne disease transmission is common, though data supporting it is scarce. here, we present data from two common mosquito species aedes aegypti (linnaeus) and culex quinquefasciatus say, biweekly sampled as adults, from march 2016 through december 2017, with bg-sentinel traps in two neighboring districts of kaohsiung city (kc), taiwan. one district has historically been a dengue transmissi ...201830104501
impact of mosquito age and insecticide exposure on susceptibility of aedes albopictus (diptera: culicidae) to infection with zika virus.zika virus (zikv) is primarily transmitted to humans by aedes aegypti and ae. albopictus. vector⁻virus interactions influencing vector competence vary and depend on biological and environmental factors. a mosquito's chronological age may impact its immune response against virus infection. insecticides, source reduction, and/or public education are currently the best defense against mosquitoes that transmit zikv. this study assessed the effects of a mosquito's chronological age at time of infecti ...201830103526
effectiveness of a field trap barrier system for controlling aedes albopictus: a "removal trapping" strategy.aedes aegypti and aedes albopictus are the main vectors for the transmission of several viral pathogens, in particular, dengue, zika and chikungunya. in the absence of vaccines and treatment, control of aedes mosquitoes is the only means of keeping these diseases in check. aedes control is difficult, and it is, therefore, necessary to evaluate the efficacy of novel control methods, particularly those targeting adult and exophilic ae. albopictus populations.201829463300
host plant forensics and olfactory-based detection in afro-tropical mosquito disease vectors.the global spread of vector-borne diseases remains a worrying public health threat, raising the need for development of new combat strategies for vector control. knowledge of vector ecology can be exploited in this regard, including plant feeding; a critical resource that mosquitoes of both sexes rely on for survival and other metabolic processes. however, the identity of plant species mosquitoes feed on in nature remains largely unknown. by testing the hypothesis about selectivity in plant feed ...201829462150
variation in wolbachia effects on aedes mosquitoes as a determinant of invasiveness and vectorial capacity.wolbachia has been introduced into aedes aegypti mosquitoes to control the spread of arboviruses, such as dengue, chikungunya and zika. studies showed that certain wolbachia strains (such as wmel) reduce replication of dengue viruses in the laboratory, prompting the release of mosquitoes carrying the bacterium into the field, where vectorial capacity can be realistically assessed in relation to native non-carriers. here we apply a new analysis to two published datasets, and show that wmel increa ...201829662096
numerical modeling of mosquito population dynamics of aedes aegypti.the global incidences of dengue virus have increased the interest in studying and understanding the mosquito population dynamics. it is predominantly spread by aedes aegypti in the tropical and sub-tropical countries in the world. understanding these dynamics is important for public health in countries where climatic and environmental conditions are favorable for the propagation of these diseases. for this reason, a new model has been proposed to investigate the population dynamics of mosquitoes ...201829661212
factors related to aedes aegypti (diptera: culicidae) populations and temperature determine differences on life-history traits with regional implications in disease transmission.aedes aegypti (l.) (diptera: culicidae) is a vector of many medically significant viruses in the americas, including dengue virus, chikungunya virus, and zika virus. traits such as longevity, fecundity, and feeding behavior contribute to the ability of ae. aegypti to serve as a vector of these pathogens. both local environmental factors and population genetics could contribute to variability in these traits. we performed a comparative study of ae. aegypti populations from four geographically and ...201829659944
detection of aedes (stegomyia) aegypti (diptera: culicidae) populations in southern alabama following a 26-yr absence and public perceptions of the threat of zika virus.with the establishment of zika virus in the americas, an accurate understanding of the geographic range of its primary vector, aedes (stegomyia) aegypti (l.) (diptera: culicidae), is vital to assessing transmission risk. in an article published in june 2016, hahn and colleagues compiled county-level records in the united states for the presence of ae. aegypti and aedes (stegomyia) albopictus (skuse) (diptera: culicidae) reported between january 1995 and march 2016. despite ecological suitability ...201829659928
coinfection with zika virus (zikv) and dengue virus results in preferential zikv transmission by vector bite to vertebrate host.several tropical cities are permissive to aedes aegypti and dengue virus (denv) endemicity and have allowed for invasion and circulation of zika virus (zikv) in the same areas. people living in arbovirus-endemic regions have been simultaneously infected with ≥2 arboviruses.201829659904
erratum to "comparative oviposition site selection in containers by aedes aegypti and aedes albopictus (diptera: culicidae) from florida". 201829618025
a comparative analysis of corpora allata-corpora cardiaca microrna repertoires revealed significant changes during mosquito metamorphosis.the corpora allata (ca) are a pair of endocrine glands with neural connections to the brain and close association with another neuroendocrine organ, the corpora cardiaca (cc). the ca from adult female aedes aegypti mosquitoes synthesize fluctuating levels of juvenile hormone (jh), which have been linked to the ovarian development and are influenced by nutritional signals. in this study, we investigated the potential involvement of micrornas (mirnas), a type of small non-coding rnas, in the regul ...201829605533
mosquito responses to trait- and density-mediated interactions of predation.mosquito and predatory larvae often share the same habitat. predators may influence mosquito prey populations through both lethal effect and non-lethal pathways. a series of experimental manipulations were used to distinguish between lethal (density-mediated interaction) and non-lethal (trait-mediated interaction) effects in a model system comprised of invasive prey mosquito, aedes aegypti, and a predatory mosquito toxorhynchites rutilus. treatments with predators present or manipulations mimick ...201829594613
effect of relaxation of deltamethrin pressure on metabolic resistance in a pyrethroid-resistant aedes aegypti (diptera: culicidae) strain harboring fixed p989p and g1016g kdr alleles.mutation of the voltage-gated sodium channel genes or knockdown resistance (kdr) and metabolic resistance in aedes aegypti (l.) (diptera: culicidae) are important resistance mechanisms against pyrethroids. the present study investigated the effect of relaxation of deltamethrin selection pressure on the level of mixed-function oxidases (mfo), when the allele frequency of s989p+v1016g mutations is fixed in a resistant ae. aegypti strain (upk-r) from chiang mai, thailand. the mosquitoes were divide ...201829590378
maternal invasion history of aedes aegypti and aedes albopictus into the isthmus of panama: implications for the control of emergent viral disease agents.despite an increase in dengue outbreaks and the arrival of chikungunya and zika disease in panama, studies on the demographic history of the invasive aedes mosquitoes that are the principle vectors of these diseases are still lacking in this region. here, we assess the genetic diversity of these mosquitoes in order to decipher their invasion histories into the isthmus of panama. dna sequences from the mitochondrial cytochrome c oxidase i gene obtained from 30 localities in 10 provinces confirmed ...201829579112
frequency and intensity of pyrethroid resistance through the cdc bottle bioassay and their association with the frequency of kdr mutations in aedes aegypti (diptera: culicidae) from mexico.the control of aedes aegypti (l.), the main urban vector that causes arboviral diseases such as dengue, chikungunya and zika, has proved to be a challenge because of a rapid increase in insecticide resistance. therefore, adequate monitoring of insecticide resistance is an essential element in the control of ae. aegypti and the diseases it transmits. we estimated the frequency and intensity (resistance frequency rapid diagnostic test [f-rdt] and resistance intensity rapid diagnostic test [i-rdt]) ...201829575404
population dynamics of engineered underdominance and killer-rescue gene drives in the control of disease vectors.a number of different genetics-based vector control methods have been proposed. two approaches currently under development in aedes aegypti mosquitoes are the two-locus engineered underdominance and killer-rescue gene drive systems. each of these is theoretically capable of increasing in frequency within a population, thus spreading associated desirable genetic traits. thus they have gained attention for their potential to aid in the fight against various mosquito-vectored diseases. in the case ...201829570717
repellent activity of trig (n-n diethyl benzamide) against man-biting mosquitoes.a study was conducted to assess efficacy of a new repellent brand trig (15% n-n diethyl benzamide) when compared to deet (20% n-n methyl toluamide). the repellents were tested in laboratory and field. in the laboratory, the repellence was tested on human volunteers, by exposing their repellent-treated arms on starved mosquitoes in cages for 3 minutes at hourly intervals, while counting the landing and probing attempts. anopheles gambiae and aedes aegypti mosquitoes were used. field evaluation wa ...201829552040
hr38, an ortholog of nr4a family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction.the aedes aegypti mosquito is the principal vector for many dangerous human viral diseases. carbohydrate metabolism (cm) is essential for supplying the energy necessary for host seeking, blood digestion and rapid egg development of this vector insect. the steroid hormone 20-hydroxyecdysone (20e) and the ecdysone receptor (ecr) are important regulators of cm, coordinating it with female reproductive events. we report here that the nr4a nuclear receptor ahr38 plays a critical role in mediating the ...201829526770
jh biosynthesis and hemolymph titers in adult male aedes aegypti mosquitoes.juvenile hormone (jh) is a major hormonal regulator in insects. in aedes aegypti females, jh signals the completion of the ecdysis to the adult stage and initiates reproductive processes. although the regulation of jh synthesis and titer in ae. aegypti females has been extensively studied, relatively little is known about changes of jh synthesis and titers in male mosquitoes, as well as on the roles of jh controlling male reproductive biology. a better understanding of male mosquito reproductive ...201829526769
mosquito pornoscopy: observation and interruption of aedes aegypti copulation to determine female polyandric event and mixed progeny.ades aegypti is the most important arbovirus vector in the world, and new strategies are under evaluation. biological studies mentioning the occurrence of a second mate in aedes aegypti can interfere with vector control program planning, which involves male mosquito release technique. this study presents different experiments to show the occurrence of mixed progeny. mixed male crosses (using a combination of different type of males in confinement with virgin females) showed no polyandric female. ...201829518112
comparative oviposition site selection in containers by aedes aegypti and aedes albopictus (diptera: culicidae) from florida.invasive container mosquito species aedes aegypti (l.) and ae. albopictus (skuse) frequently co-occur in nature where they are typically segregated spatially by distinctive macrohabitat preferences that may select for different oviposition strategies. in a standard cage environment, we compared oviposition site selection by individual gravid females of these species exposed to variable numbers of water-holding cups and the presence or absence of a rugose wettable container lining. offered plasti ...201829514300
building infestation index for aedes aegypti and occurrence of dengue fever in the municipality of foz do iguaçu, paraná, brazil, from 2001 to 2016.the building infestation index (bii) uses the rapid assay of the larval index for aedes aegypti (liraa) to express the relationship between positive and surveyed properties. we evaluated liraa and the relationship between the bii and climate variables for dengue cases in foz do iguaçu municipality, paraná.201829513847
occurrence of sweet refuse at disposal sites: rainwater retention capacity and potential breeding opportunities for aedes aegypti.nectar is the staple diet of adult mosquitoes in the wild, but its availability is inconsistent and can be affected by rainfall. in urban centers, aedes vectors commonly use man-made containers as their major habitat; however, they can colonize any items replenished by rainfall. garbage output has increased significantly in recent years, at a time when collection frequency is reducing. such garbage usually includes organic components, some of which are sweet and can be fed upon by other animals ...201829512008
the non-canonical notch signaling is essential for the control of fertility in aedes aegypti.the notch signaling pathway is a highly evolutionarily-conserved cell-cell signaling pathway that regulates many events during development. it plays a pivotal role in the regulation of fundamental cellular processes, such as cell proliferation, stem cell maintenance, and differentiation during embryonic and adult development. however, functions of notch signaling in aedes aegypti, the major mosquito vector for dengue, are largely unknown. in this study, we identified a unique feature of a. aegyp ...201829505577
dengue knowledge in indoor dengue patients from low socioeconomic class; aetiology, symptoms, mode of transmission and prevention.dengue fever has emerged as an emerging public health issue during last decade bearing significant morbidity and economic burden particularly in third world countries. current study aims to assess various domains of knowledge of indoor dengue patients..201829504327
resistance of aedes aegypti (diptera: culicidae) populations to deltamethrin, permethrin, and temephos in cambodia.dengue fever is a major public health concern, including 185,000 annual cases in cambodia. aedes aegypti is the primary vector for dengue transmission and is targeted with insecticide treatments. this study characterized the insecticide resistance status of ae aegypti from rural and urban locations. the susceptibility to temephos, permethrin, and deltamethrin of ae aegypti was evaluated in accordance with world health organization instructions. all the field populations showed lower mortality ra ...201829502428
bacterial microbiota of aedes aegypti mosquito larvae is altered by intoxication with bacillus thuringiensis israelensis.insect microbiota is a dynamic microbial community that can actively participate in defense against pathogens. bacillus thuringiensis (bt) is a natural entomopathogen widely used as a bioinsecticide for pest control. although bt's mode of action has been extensively studied, whether the presence of microbiota is mandatory for bt to effectively kill the insect is still under debate. an association between a higher tolerance and a modified microbiota was already evidenced but a critical point rema ...201829499735
anti-diuretic action of a capa neuropeptide against a subset of diuretic hormones in the disease vector aedes aegypti.the mosquito aedes aegypti is a vector responsible for transmitting various pathogens to humans, and their prominence as chief vectors of human disease is largely due to their anthropophilic blood feeding behaviour. larval stage mosquitoes must deal with the potential dilution of their haemolymph in freshwater, whereas the haematophagus a. aegypti female faces the challenge of excess ion and water intake after a blood meal. the excretory system, composed of the malpighian tubules (mts) and hindg ...201829496779
dengue in araraquara, state of são paulo: epidemiology, climate and aedes aegypti infestation.objective to describe the epidemiology of dengue in a medium-sized city in the state of são paulo. methods data, such as circulating serotypes, severe cases and deaths, age group, sex, among others, were obtained on reported and confirmed dengue cases in araraquara, state of são paulo, between 1991 and 2015. climatic and infestation data were also analyzed. these variables were evaluated descriptively, using statistical measures such as frequencies, averages, minimum and maximum. dengue incidenc ...201829489994
truck-mounted natular 2ec (spinosad) ulv residual treatment in a simulated urban environment to control aedes aegypti and aedes albopictus in north florida.preemptive treatment of dry habitats with an ultra-low volume (ulv) residual larvicide may be effective in an integrated vector management program to control populations of container-inhabiting aedes mosquitoes, key vectors of zika, dengue, and chikungunya viruses. we exposed dry, artificial containers placed in exposed and protected locations to natular 2ec (spinosad) larvicide applied with a truck-mounted ulv sprayer in a simulated urban setting in north florida, and later introduced water and ...201831442121
ultra-low volume application of spinosad (natular 2ec) larvicide as a residual in a tropical environment against aedes and anopheles species.we investigated the efficacy of a liquid larvicide, natular 2ec® (spinosad), applied with ultra-low volume sprayer as a residual application during the dry season in southeastern thailand against 4 medically important species-aedes aegypti, ae. albopictus, anopheles dirus, and an. minimus. we found that this larvicide could be applied as a residual to dry areas known to collect water and potentially still be effective after rains or irrigation, which could increase the flexibility and efficiency ...201831442120
effect of travel speed on dispersion of aqualuer 20-20 sprayed by a truck-mounted ultra-low-volume sprayer against caged aedes aegypti1.the effect of travel speed of a truck-mounted ultra-low-volume (ulv) sprayer on its application efficacy was studied at st. johns county fairground, elkton, fl, during summer 2015. the efficacy was assessed by spray deposition, droplet size spectrum, and 24-h mortality of caged adult aedes aegypti, using 2 rows of sampling locations, 15 m apart and spread up to 122 m from the spray. each location had a bioassay cage and an impinger droplet sampler, 1 m apart from each other, at 1.5 m off the gro ...201831442115
application efficacy of vectobac wdg against larval aedes aegypti using thermal fog technology.the effectiveness of thermal fog application of bacillus thuringiensis israelensis (vectobac® wdg) against larval aedes aegypti was evaluated in open and forest canopied environments in northeastern florida, using a handheld igeba® tf34 thermal fogger (with water conversion kit). the product was applied at the maximum label rate of 1 kg/ha to linear transects, in each environment, containing empty 0.5-liter cups. after application, late-2nd to early-3rd instars of ae. aegypti were added to each ...201831442110
ultra-low volume application of spinosad (natular 2ec) as a residual in a hot-arid environment against aedes aegypti.the invasive aedes aegypti is an important disease vector increasing in frequency in hot-arid regions of the usa such as the southwest. within hot-arid surroundings this mosquito may be confined to peridomestic locations that tend to be cooler and humid, such as in lush, irrigated ornamental vegetation surrounding homes. however, to reach these habitat refugia, ultra-low volume (ulv) applications of insecticides targeting this mosquito must retain efficacy after being sprayed from the air or str ...201831442108
mosquitocidal activity of a naturally occurring isochroman and synthetic analogs from the plant pathogenic fungus, diaporthe eres against aedes aegypti (diptera: culicidae).the culture filtrate of a plant pathogenic fungus that infects english ivy (hedera helix l., araliaceae) was investigated for mosquitocidal constituents by bioassay-guided isolation. the fungus responsible for pathogenic effects on the plant h. helix has been identified as diaporthe eres nitschke by molecular techniques. the mosquito adulticidal constituent in the culture filtrate was identified as 3,4-dihydro-8-hydroxy-3,5-dimethylisocoumarin (1) by spectroscopic techniques. laboratory bioassay ...201829474636
comparative analysis of major mosquito vectors response to seed-derived essential oil and seed pod-derived extract from acacia nilotica.botanical metabolites are increasingly realized as potential replacements to chemical insecticides. in the present study, acacia nilotica seed essential oil and seed pod solvent extracts were tested for bioefficacy against three important types of mosquitoes. mortality was recorded 24 h post-treatment, while smoke toxicity of adult mosquitoes was recorded at 10 min intervals for 40 min. seed pod powder was extracted with different solvents and hydrodistilled seed oil chemical constituents were d ...201829473901
socioeconomic and human behavioral factors associated with aedes aegypti (diptera: culicidae) immature habitat in tucson, az.aedes aegypti (l.; diptera: culicidae) has been established in the southwestern united states for several decades, but relationships between humans and mosquitoes in this arid region are not well-characterized. in august 2012, the outdoor premises of 355 houses within 20 neighborhoods in tucson, arizona were surveyed for containers that could provide larval habitat for ae. aegypti mosquitoes. at the same time, a knowledge, attitudes and practices (kap) questionnaire was administered to a residen ...201829471405
regulation of midgut cell proliferation impacts aedes aegypti susceptibility to dengue virus.aedes aegypti is the vector of some of the most important vector-borne diseases like dengue, chikungunya, zika and yellow fever, affecting millions of people worldwide. the cellular processes that follow a blood meal in the mosquito midgut are directly associated with pathogen transmission. we studied the homeostatic response of the midgut against oxidative stress, as well as bacterial and dengue virus (denv) infections, focusing on the proliferative ability of the intestinal stem cells (isc). i ...201829782512
zika virus: report from the task force on tropical diseases by the world federation of societies of intensive and critical care medicine.zika fever is an acute infectious disease caused by the zika virus (zikv), an rna virus belonging to the genus flavivirus. zikv can be transmitted by bites from aedes aegypti and aedes albopictus species, sexual activity, blood transfusion and from mother to fetus during pregnancy and delivery. a total of 50 territories and countries in the americas had reported zikv infections. for those with symptoms, the presentation is similar to dengue fever with body aches, joint pain, fatigue, malaise, fe ...201829779827
genome sequence analysis of a novel bacillus thuringiensis strain blb406 active against aedes aegypti larvae, a novel potential bioinsecticide.blb406 is a novel isolate of bacillus thuringiensis with a larvicidal activity against aedes aegypti larvae. it displays original plasmidic and crystal protein patterns. the present work reported molecular and bioinformatic analyses for the genome sequence of blb406 using miseq illumina next-generation sequencing technology. the reads were assembled by velvet tool. using rast program and pgaap, the genome of blb406 strain was shown to contain 6297 genes corresponding to 5924 protein coding seque ...201829778876
chemical profile and pharmacological effects of the resin and essential oil from bursera slechtendalii: a medicinal "copal tree" of southern mexico.this study reports on the volatile profile and pharmacological activities of the essential oil and resin from bursera slechtendalii, an aromatic medicinal tree commercialized in southern mexico. the essential oil contained 25 volatiles including p-cymen-8-ol (25.4-35.7%), n-hexyl hexanoate (6.8-17.6%) and n-octyl hexanoate (7.6-14.8%), whereas the crystallized resin contained 14 volatiles, including n-octyl acetate (29.6-34.2%), limonene (15.8-18.7%) and n-octyl butyrate (12.6-17-6%). the inhala ...201829777752
modeling dengue vector population using remotely sensed data and machine learning.mosquitoes are vectors of many human diseases. in particular, aedes ægypti (linnaeus) is the main vector for chikungunya, dengue, and zika viruses in latin america and it represents a global threat. public health policies that aim at combating this vector require dependable and timely information, which is usually expensive to obtain with field campaigns. for this reason, several efforts have been done to use remote sensing due to its reduced cost. the present work includes the temporal modeling ...201829777650
use of bead-based serologic assay to evaluate chikungunya virus epidemic, haiti.the index case of chikungunya virus (chikv) in haiti was reported during early 2014; the vector, the pervasive aedes aegypti mosquito, promoted rapid spread throughout the country. during december 2014-february 2015, we collected blood samples from 4,438 persons at 154 sites (62 urban, 92 rural) throughout haiti and measured chikv igg by using a multiplex bead assay. overall chikv seroprevalence was 57.9%; differences between rural (mean 44.9%) and urban (mean 78.4%) areas were pronounced. logis ...201829774861
co-breeding association of aedes albopictus (skuse) and aedes aegypti (linnaeus) (diptera: culicidae) in relation to location and container size.the occurrence of major outbreaks of dengue, and other vector borne diseases such as chikungunya and zika in tropical and subtropical regions has rendered control of the diseases a top-priority for many affected countries including malaysia. control of the mosquito vectors aedes aegypti and aedes albopictus through the reduction of breeding sites and the application of insecticides to kill immature forms and adults are the main control efforts to combat these diseases. the present study describe ...201829644025
evaluation of a simple polytetrafluoroethylene (ptfe)-based membrane for blood-feeding of malaria and dengue fever vectors in the laboratory.controlled blood-feeding is essential for maintaining laboratory colonies of disease-transmitting mosquitoes and investigating pathogen transmission. we evaluated a low-cost artificial feeding (af) method, as an alternative to direct human feeding (dhf), commonly used in mosquito laboratories.201829642937
yellow fever.the yellow fever (yf) virus is a flavivirus, transmitted by haemagogus, sabethes or aedes aegypti mosquitoes. the disease is endemic in forest areas in africa and latin america leading to epizootics in monkeys that constitute the reservoir of the disease. there are two forms of yf: sylvatic, transmitted accidentally when approaching the forests, and urban, which can be perpetuated by aedes aegypti. in brazil, the last case of urban yf occurred in 1942. since then, there has been an expansion of ...201829641667
identification of entomopathogenic nematodes and symbiotic bacteria from nam nao national park in thailand and larvicidal activity of symbiotic bacteria against aedes aegypti and aedes albopictus.entomopathogenic nematodes (epns) that are symbiotically associated with xenorhabdus and photorhabdus bacteria can kill target insects via direct infection and toxin action. there are limited reports identifying such organisms in the national park of thailand. therefore, the objectives of this study were to identify epns and symbiotic bacteria from nam nao national park, phetchabun province, thailand and to evaluate the larvicidal activity of bacteria against aedes aegypti and ae. albopictus. a ...201829641570
suppression of the pelo protein by wolbachia and its effect on dengue virus in aedes aegypti.the endosymbiont wolbachia is known to block replication of several important arboviruses, including dengue virus (denv), in the mosquito vector aedes aegypti. so far, the exact mechanism of this viral inhibition is not fully understood. a recent study in drosophila melanogaster has demonstrated an interaction between the pelo gene and drosophila c virus. in this study, we explored the possible involvement of the pelo protein, that is involved in protein translation, in wolbachia-mediated antivi ...201829641562
exploring mosquito fauna of majuro atoll (republic of marshall islands) in the context of zika outbreak.first autochthonous zika clinical case was reported in the republic of marshall islands (rmi) on majuro atoll in february 2016. an entomological survey of mosquito larvae and adult populations was carried out in four areas of majuro, the most populated atoll of rmi encompassing different habitats (forest, rural, or urban) including some with confirmed clinical zika cases to evaluate which mosquito species could be involved in the zika transmission. a total of 2,367 immature and adult mosquito sp ...201829635382
a sex-structured model with birth pulse and release strategy for the spread of wolbachia in mosquito population.dengue fever is one of the most important diseases causing illness and death all over the world, which brings tremendous threat to peoples' life and property security, especially in the undeveloped areas. the main vector, aedes aegypti, must be controlled to prevent the transmission of dengue. there are a variety of methods to control it. wolbachia is an innovative bacterium which breaks the dengue transmission cycle for its characteristics of cytoplasmic incompatibility and maternal transmissio ...201829625205
efficacy of aedes aegypti control by indoor ultra low volume (ulv) insecticide spraying in iquitos, peru.aedes aegypti is a primary vector of dengue, chikungunya, zika, and urban yellow fever viruses. indoor, ultra low volume (ulv) space spraying with pyrethroid insecticides is the main approach used for ae. aegypti emergency control in many countries. given the widespread use of this method, the lack of large-scale experiments or detailed evaluations of municipal spray programs is problematic.201829624581
the influence of urban heat islands and socioeconomic factors on the spatial distribution of aedes aegypti larval habitats.we addressed the potential associations among the temporal and spatial distribution of larval habitats of aedes (stegomyia) aegypti, the presence of urban heat islands and socioeconomic factors. data on larval habitats were collected in santa bárbara d'oeste, são paulo, brazil, from 2004 to 2006, and spatial and temporal variations were analysed using a wavelet-based approach. we quantified urban heat islands by calculating surface temperatures using the results of wavelet analyses and grey leve ...201829772891
urban environmental clustering to assess the spatial dynamics of aedes aegypti breeding sites.the identification of aedes aegypti breeding hotspots in urban areas is crucial for the rational design of control strategies against this disease vector. remote sensing and geographic information systems offer valuable tools for mapping habitat suitability of a given area. however, predicting species occurrences by means of probability distribution maps based on transversal entomological surveys has limited utility for local authorities. the aim of the present study was to carefully examine the ...201829772886
encapsidated host factors in alphavirus particles influence midgut infection of aedes aegypti.transmission of mosquito-borne viruses requires the efficient infection of both a permissive vertebrate host and a competent mosquito vector. the infectivity of sindbis virus (sinv), the type species of the alphavirus genus, is influenced by both the original and new host cell. we have shown that infection of vertebrate cells by sinv, chikungunya virus (chikv), and ross river virus (rrv) produces two subpopulations of virus particles separable based on density. in contrast, a single population o ...201829772674
tracking aedes aegypti (diptera: culicidae) larval behavior across development: effects of temperature and nutrients on individuals' foraging behavior.immature mosquitoes alter their foraging behavior in response to variation in nutrients, predators, and temperature, with consequences on the adult stage where pathogens are transmitted. these patterns of behavior have not been described with respect to both developmental stage and environmental variation, nor has behavior been examined within an individual across instars. we hypothesized that individual larvae have distinct behavioral syndromes, and predict that the rank of foraging activity in ...201829771372
niche conservatism of aedes albopictus and aedes aegypti - two mosquito species with different invasion histories.biological invasions have been associated with niche changes; however, their occurrence is still debated. we assess whether climatic niches between native and non-native ranges have changed during the invasion process using two globally spread mosquitoes as model species, aedes albopictus and aedes aegypti. considering the different time spans since their invasions (>300 vs. 30-40 years), niche changes were expected to be more likely for ae. aegypti than for ae. albopictus. we used temperature a ...201829769652
ornamental bromeliads of miami-dade county, florida are important breeding sites for aedes aegypti (diptera: culicidae).a major public health concern is the emergence and geographical spread of vector-borne diseases such as zika and yellow fever. ornamental bromeliads retaining water in their leaf axils represent potential breeding sites for mosquitoes. as the role of ornamental bromeliads in breeding aedes aegypti in miami-dade county, florida is unknown, we hypothesize that ornamental bromeliads are important breeding sites for ae. aegypti. our objective was to survey bromeliads in areas with high densities of ...201829769105
efficacy of the main repellents available in the brazilian market against aedes aegypti bites under concentrations applied to pediatric populations. 201829768569
ns1 codon usage adaptation to humans in pandemic zika virus.zika virus (zikv) was recognised as a zoonotic pathogen in africa and southeastern asia. human infections were infrequently reported until 2007, when the first known epidemic occurred in micronesia. after 2013, the asian lineage of zikv spread along the pacific islands and americas, causing severe outbreaks with millions of human infections. the recent human infections of zikv were also associated with severe complications, such as an increase in cases of guillain-barre syndrome and the emergenc ...201829768530
pyrethroid insecticides maintain repellent effect on knock-down resistant populations of aedes aegypti mosquitoes.pyrethroid-treated clothing is commonly worn for protection against mosquitoes; pyrethroids are both insecticides and repellents. pyrethroid resistance has become increasingly common in aedes aegypti, the vector of dengue, zika, and other arboviruses, but it is not clear whether resistance is associated with reductions in repellency. in order to determine whether long-lasting permethrin impregnated (llpi) clothing is protective, we used aedes aegypti from new orleans, la (pyrethroid-sensitive) a ...201829763445
effects of light and temperature on daily activity and clock gene expression in two mosquito disease vectors.most organisms feature an endogenous circadian clock capable of synchronization with their environment. the most well-known synchronizing agents are light and temperature. the circadian clock of mosquitoes, vectors of many pathogens, drives important behaviors related to vectoral capacity, including oviposition, host seeking, and hematophagy. main clock gene expression, as well as locomotor activity patterns, has been identified in aedes aegypti and culex quinquefasciatus under artificial light- ...201829761749
threats of zika virus transmission for asia and its hindu-kush himalayan region.asia and its hindu kush himalayan (hkh) region is particularly vulnerable to environmental change, especially climate and land use changes further influenced by rapid population growth, high level of poverty and unsustainable development. asia has been a hotspot of dengue fever and chikungunya mainly due to its dense human population, unplanned urbanization and poverty. in an urban cycle, dengue virus (denv) and chikungunya virus (chikv) are transmitted by aedes aegypti and ae. albopictus mosqui ...201829759076
aedes aegypti(linnaeus) larvae from dengue outbreak areas in selangor showing resistance to pyrethroids but susceptible to organophosphates.the resistance status of selangor aedes aegypti (linnaeus) larvae against four major groups of insecticides (i.e., organochlorines, carbamates, organophosphates and pyrethroids) was investigated. aedes aegypti were susceptible against temephos (organophosphate), although resistance (rr50 = 0.21-2.64) may be developing. the insecticides susceptibility status of ae. aegypti larvae were found heterogeneous among the different study sites. results showed that ae. aegypti larvae from klang, sabak ber ...201829758171
a review of the ongoing research on zika virus treatment.the zika fever is an arboviral disease resulting from the infection with zika virus (zikv). the virus is transmitted to humans by the bite of aedes mosquitos, mainly aedes aegypti and aedes albopictus. zikv has been detected for decades in african and asian regions and, since 2007, has spread to other continents; among them, infections are most reported in the americas. this can be explained by the presence of vectors in highly populated and tropical regions where people are susceptible to conta ...201829758005
two novel mutations in the voltage-gated sodium channel associated with knockdown resistance (kdr) in the dengue vector aedes aegypti in vietnam. 201829757514
diel patterns of aedes aegypti (diptera: culicidae) after resurgence in st. augustine, florida as collected by a mechanical rotator trap. 201829757509
mosquito traps for urban surveillance: collection efficacy and potential for use by citizen scientists.mosquito-borne diseases are a pervasive public health problem on a global scale, and effective management of them requires well-designed surveillance programs for both vectors and pathogens. mosquito traps are a common component of such programs, and their reach can be expanded by engaging citizen scientists. in this study in a southern australian city, we compared the mosquito collection efficacy of two types of traps and assessed their suitability for use in citizen science programs. bg sentin ...201829757507
controversies over the scientific name of the principal mosquito vector of yellow fever virus - expediency versus validity.the history of the scientific name of the yellow fever mosquito, the vector of yellow fever virus, ranges from 1757 to the early twenty-first century. in his 1757 work iter palaestinum, frederic hasselquist gave the name culex aegypti to a mosquito species responsible for fierce attacks on humans in egypt. that name was never later ascribed to hasselquist as author, but to linnaeus, although the name never appeared in any of linnaeus' publications. in cuba, at the end of the nineteenth century, ...201829757506
effects of scarcity and excess of larval food on life history traits of aedes aegypti (diptera: culicidae).few studies have assessed the effects of food scarcity or excess on the life history traits of aedes aegypti (l.) (diptera: culicidae) independently from larval density. we assessed immature survival, development time, and adult size in relation to food availability. we reared cohorts of 30 ae. aegypti larvae from newly hatched to adult emergence with different food availability. food conditions were kept constant by transferring larvae each day to a new food solution. immature development was c ...201829757503
de novo dna synthesis in aedes aegypti midgut cells as a complementary strategy to limit dengue viral replication.aedes aegypti is the main vector of dengue virus, carrying the virus during the whole mosquito life post-infection. few mosquito fitness costs have been associated to the virus infection, thereby allowing for a swift dissemination. in order to diminish the mosquito population, public health agency use persistent chemicals with environmental impact for disease control. most countries barely use biological controls, if at all. with the purpose of developing novel dengue control strategies, a detai ...201829755433
sargassum wightii-synthesized zno nanoparticles - from antibacterial and insecticidal activity to immunostimulatory effects on the green tiger shrimp penaeus semisulcatus.the green synthesis of metal nanoparticles using phytochemical from marine seaweeds is a fast-growing research field in nanotechnology. here, the biosynthesis of zinc oxide nanoparticles was achieved using the hot water extract of sargassum wightii. the hot water extract prepared from s. wightii (h sw) and zno nps were studied by uv-visible and ftir spectroscopy, sem and xrd. then, both products were evaluated for antibiofilm activity towards aquatic pathogens. the nanoparticles' immunostimulati ...201829754050
dynamics of dengue disease with human and vector mobility.dengue is a vector borne disease transmitted to humans by aedes aegypti mosquitoes carrying virus of different serotypes. dengue exhibits complex spatial and temporal dynamics, influenced by various biological, human and environmental factors. in this work, we study the dengue spread for a single serotype (denv-1) including statistical models of human mobility with exponential step length distribution, by using reaction-diffusion equations and stochastic cellular automata (sca) approach. we anal ...201829751893
a wolbachia triple-strain infection generates self-incompatibility in aedes albopictus and transmission instability in aedes aegypti.artificially-introduced transinfections of the intracellular bacterium wolbachia pipientis have the potential to reduce the vectorial capacity of mosquito populations for viruses such as dengue and chikungunya. aedes albopictus has two native strains of wolbachia, but their replacement with the non-native wmel strain blocks transmission of both viruses. the pattern of cytoplasmic incompatiiblity generated by wmel with wild-types is bidirectional. novel-plus-native-strain co-infection is predicte ...201829751814
molecular responses to the zika virus in mosquitoes.the zika virus (zikv), originally discovered in 1947, did not become a major concern until the virus swept across the pacific and into the americas in the last decade, bringing with it news of neurological complications and birth defects in zikv affected areas. this prompted researchers to dissect the molecular interactions between zikv and the mosquito vector in an attempt to better understand not only the changes that occur upon infection, but to also identify molecules that may potentially en ...201829751526
effect of uv-b irradiation on water-suspended metarhizium anisopliae s.l. (hypocreales: clavicipitaceae) conidia and their larvicidal activity in aedes aegypti (diptera: culicidae).ultraviolet (uv) radiation is a key limiting factor for biological pest control with entomopathogenic fungi. while little is known about the impact of uv on metarhizium anisopliae metchnikoff (sorokin) (hypocreales: clavicipitaceae) conidia in aquatic mosquito-breeding sites, this study determined the effect of uv-b on the viability and virulence of m. anisopliae sensu lato (s.l.) strain ip 46 in the laboratory against aedes aegypti (l.) (diptera: culicidae) larvae. conidia were treated in cups ...201829750411
experimental zika virus infection in aedes aegypti: susceptibility, transmission & co-infection with dengue & chikungunya viruses.there are reports about the susceptibility of aedes mosquitoes to zikv from various countries, however, no such information is available from indian sub-continent, although, high level of group cross-reactivity of zikv with other flaviviruses has been reported. during outbreak situations, many cases of dengue (den) and chikungunya (chik) are reported. in such scenario, vector mosquitoes are likely to get co-infection/secondary-infection with one or other virus. the present study was carried out ...201829749366
a new "american" subgroup of african-lineage chikungunya virus detected in and isolated from mosquitoes collected in haiti, 2016.as part of on-going arboviral surveillance activity in a semi-rural region in haiti, chikungunya virus (chikv)-positive mosquito pools were identified in 2014 (the peak of the caribbean asian-clade epidemic), and again in 2016 by rt-pcr. in 2014, chikv was only identified in aedes aegypti (11 positive pools/124 screened). in contrast, in sampling in 2016, chikv was not identified in ae. aegypti, but, rather, in (a) a female aedes albopictus pool, and (b) a female culex quinquefasciatus pool. gen ...201829746539
seasonal temperature variation influences climate suitability for dengue, chikungunya, and zika transmission.dengue, chikungunya, and zika virus epidemics transmitted by aedes aegypti mosquitoes have recently (re)emerged and spread throughout the americas, southeast asia, the pacific islands, and elsewhere. understanding how environmental conditions affect epidemic dynamics is critical for predicting and responding to the geographic and seasonal spread of disease. specifically, we lack a mechanistic understanding of how seasonal variation in temperature affects epidemic magnitude and duration. here, we ...201829746468
atypical neurological manifestations of chikungunya fever: two case reports.chikungunya fever (chik) is an arboviral disease which is transmitted by a mosquito bite (aedes aegypti). fever, polyarthralgia, and maculopapular rash are hallmark clinical manifestations of this disease. these manifestations are self-liming and complete recovery is seen in most of the cases. however, atypical and lethal complications such as myocarditis, hepatitis, guillain-barre syndrome (gbs), and meningoencephalitis have been reported in few cases of chik. we hereby report two cases of chik ...201829743772
complete genome of aedes aegypti anphevirus in the aag2 mosquito cell line.a novel negative-sense rna virus, aedes aegypti anphevirus, was recently identified in wild aedes aegypti mosquitoes. we show that this virus is also present in the aag2 aedes aegypti cell line and characterize its complete genome and evolutionary history. the aedes aegypti anphevirus genome is estimated to be 12 916 nucleotides in length, contains four genes and has a genome structure similar to that of other anpheviruses. phylogenetically, aedes aegypti anphevirus falls within an unclassified ...201829741476
expression of a synthetic gene for the major cytotoxin (cyt1aa) of bacillus thuringiensis subsp. israelensis in the chloroplast of wild-type chlamydomonas.chlamydomonas reinhardtii (chlamydomonas) strains that are toxic to mosquito larvae because they express chloroplast transgenes that are based on the mosquitocidal proteins of bacillus thuringiensis subsp. israelensis (bti) could be very useful in mosquito control. chlamydomonas has several advantages for this approach, including genetic controls not generally available with industrial algae. the bti toxin is produced by sporulating bacteria and has been used for mosquito control for >30 years w ...201829738473
crystal structure of a novel kunitz type inhibitor, alocasin with anti-aedes aegypti activity targeting midgut proteases.the pesticidal properties of many kunitz-type inhibitors have been reported previously; however, the mechanism of action is not well established. in this study, the activity of alocasin against aedes aegypti is demonstrated and the structure-activity relationship of this kunitz-type inhibitor is explained through x-ray structure analyses.201829737039
antibodies against chikungunya in northern mozambique during dengue outbreak, 2014.an outbreak of dengue and high densities of aedes aegypti were reported in 2014 in northern mozambique, suggesting an increased risk for other arboviruses such as chikungunya virus (chikv) in this region. the aim of this study was to investigate the occurrence of chikv during an outbreak of dengue virus (denv) in pemba city in northern mozambique in 2014. febrile patients (n = 146) seeking medical attention at the pemba provincial hospital between march and april 2014 were enrolled in this study ...201829733254
male contributions during mating increase female survival in the disease vector mosquito aedes aegypti.aedes aegypti is a vector of medically important viruses including those causing zika, dengue, and chikungunya. during mating, males transfer a number of proteins and other molecules to the female and these components of the male ejaculate are essential in shifting female post-mating behaviors in a number of insect species. because these molecules are highly variable by species, and female post-mating behavior by species is also varied, behavioral assays testing the function of the ejaculate are ...201829729859
improving the delivery and efficiency of fungus-impregnated cloths for control of adult aedes aegypti using a synthetic attractive lure.entomopathogenic fungi are highly promising agents for controlling aedes aegypti mosquitoes. deploying fungus-impregnated black cloths in pet traps efficiently reduced ae. aegypti female survival rates under intra-domicile conditions. with the aim of further increasing the effectiveness of the traps, the addition of attractive lures to fungus-impregnated traps was evaluated.201829728121
advising a woman with suspected zika virus infection.the zika virus (zikv) epidemic is spreading: 67 countries are now reporting transmission, and over 2,000 cases of congenital zika syndrome (czs) have been confirmed. the heaviest burden has been borne by those living where poverty, poor infrastructure, and lack of access to health services are common and the penetration of aedes aegypti is high. because most cases are asymptomatic, the most dramatic signs of the disease appear through the czs cases. in spite of the need for disaggregated epidemi ...201829724698
larval density mediates knockdown resistance to pyrethroid insecticides in adult aedes aegypti.understanding mechanisms driving insecticide resistance in vector populations remains a public health priority. to date, most research has focused on the genetic mechanisms underpinning resistance, yet it is unclear what role environmental drivers may play in shaping phenotypic expression. one of the key environmental drivers of aedes aegypti mosquito population dynamics is resource-driven intraspecific competition at the larval stage. we experimentally investigated the role of density-dependent ...201829724237
contributions from the silent majority dominate dengue virus transmission.despite estimates that, each year, as many as 300 million dengue virus (denv) infections result in either no perceptible symptoms (asymptomatic) or symptoms that are sufficiently mild to go undetected by surveillance systems (inapparent), it has been assumed that these infections contribute little to onward transmission. however, recent blood-feeding experiments with aedes aegypti mosquitoes showed that people with asymptomatic and pre-symptomatic denv infections are capable of infecting mosquit ...201829723307
wolbachia significantly impacts the vector competence of aedes aegypti for mayaro virus.wolbachia, an intracellular endosymbiont present in up to 70% of all insect species, has been suggested as a sustainable strategy for the control of arboviruses such as dengue, zika and chikungunya. as mayaro virus outbreaks have also been reported in latin american countries, the objective of this study was to evaluate the vector competence of brazilian field-collected ae. aegypti and the impact of wolbachia (wmel strain) upon this virus. our in vitro studies with aag2 cells showed that mayaro ...201829720714
vector competence of certain culex and aedes mosquitoes for the chittoor virus, the indian variant of the batai virus.chittoor virus (chitv), a mosquito-borne bunyavirus (orthobunyavirus: bunyaviridae) isolated in india, has been found to be antigenically close to the batai virus (batv), which has a wide distribution across asia, europe, and africa. the latter virus causes influenza-like illness in humans and mild illness in sheep and goats. batv has been involved in genetic reassortment with other bunyaviruses, generating novel genome combinations and causing severe clinical manifestations including hemorrhagi ...201829718685
high throughput discovery of protein variants using proteomics informed by transcriptomics.proteomics informed by transcriptomics (pit), in which proteomic ms/ms spectra are searched against open reading frames derived from de novo assembled transcripts, can reveal previously unknown translated genomic elements (tges). however, determining which tges are truly novel, which are variants of known proteins, and which are simply artefacts of poor sequence assembly, is challenging. we have designed and implemented an automated solution that classifies putative tges by comparing to referenc ...201829718325
dehydration prompts increased activity and blood feeding by mosquitoes.current insights into the mosquito dehydration response rely on studies that examine specific responses but ultimately fail to provide an encompassing view of mosquito biology. here, we examined underlying changes in the biology of mosquitoes associated with dehydration. specifically, we show that dehydration increases blood feeding in the northern house mosquito, culex pipiens, which was the result of both higher activity and a greater tendency to land on a host. similar observations were noted ...201829717151
efficacy of larvicides for the control of dengue, zika, and chikungunya vectors in an urban cemetery in southern mexico.many countries in latin america have recently experienced outbreaks of zika and chikungunya fever, in additional to the usual burden imposed by dengue, all of which are transmitted by aedes aegypti in this region. to identify potential larvicides, we determined the toxicity of eight modern insecticides to a. aegypti larvae from a colony that originated from field-collected insects in southern mexico. the most toxic compounds were pyriproxyfen (which prevented adult emergence) and λ-cyhalothrin, ...201829713901
parallel evolution of vgsc mutations at domains is6, iis6 and iiis6 in pyrethroid resistant aedes aegypti from mexico.aedes aegypti is the primary urban mosquito vector of viruses causing dengue, zika and chikungunya fevers -for which vaccines and effective pharmaceuticals are still lacking. current strategies to suppress arbovirus outbreaks include removal of larval-breeding sites and insecticide treatment of larval and adult populations. insecticidal control of ae. aegypti is challenging, due to a recent rapid global increase in knockdown-resistance (kdr) to pyrethroid insecticides. widespread, heavy use of p ...201829712956
identification of candidate iron transporters from the zip/znt gene families in the mosquito aedes aegypti.mosquito-transmitted viral pathogens, such as dengue and zika, afflict tens of thousands of people every year. these viruses are transmitted during the blood-feeding process that is required for mosquito reproduction, the most important vector being aedes aegypti. while vertebrate blood is rich in protein, its high iron content is potentially toxic to mosquitoes. although iron transport and sequestration are essential in the reproduction of vector mosquitoes, we discovered that culicine mosquito ...201829706902
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