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transcriptomic analysis of aedes aegypti in response to mosquitocidal bacillus thuringiensis llp29 toxin.globally, aedes aegypti is one of the most dangerous mosquitoes that plays a crucial role as a vector for human diseases, such as yellow fever, dengue, and chikungunya. to identify (1) transcriptomic basis of midgut (2) key genes that are involved in the toxicity process by a comparative transcriptomic analysis between the control and bacillus thuringiensis (bt) toxin (llp29 proteins)-treated groups. next-generation sequencing technology was used to sequence the midgut transcriptome of a. aegypt ...201830140020
modelling the distribution of the vector aedes aegypti in a central argentine city.aedes aegypti (diptera: culicidae) is an urban mosquito involved in the transmission of numerous viruses, including dengue, chikungunya and zika. in argentina, ae. aegypti is the main vector of dengue virus and has been involved in several outbreaks in regions ranging from northern to central argentina since 2009. in order to evaluate areas of potential vector-borne disease transmission in the city of córdoba, argentina, the present study aimed to identify the environmental, socioeconomic and de ...201830027565
assessment of insecticide resistance in primary dengue vector, aedes aegypti (linn.) from northern districts of west bengal, india.aedes mosquitoes are the major vectors transmitting several arboviral diseases such as dengue, zika and chikungunya worldwide. northern districts of west bengal is home to several epidemics vectored by mosquito including dengue infections, proper control of which depends on efficient vector control. however the onset of insecticide resistance has resulted in failure of vector control approaches. this study was carried out to unveil the level of insecticide resistance prevailing among the primary ...201830026024
copy number variation (cnv) and insecticide resistance in mosquitoes: evolving knowledge or an evolving problem?copy number variation (cnv) in insect genomes is a rich source of potentially adaptive polymorphism which may help overcome the constraints of purifying selection on conserved genes and/or permit elevated transcription. classic studies of amplified esterases and acetylcholinesterase duplication in culex pipiens quantified evolutionary dynamics of cnv driven by insecticidal selection. a more complex and potentially medically impactful form of cnv is found in anopheles gambiae, with both heterogen ...201830025639
melanization of dirofilaria immitis larvae in different culicid species.dirofilaria immitis is an important filarioid transmitted by culicids. the vector role of these arthropods may be influenced by biological events as melanization against d. immitis larvae. this study aimed to detect the occurrence of melanization in culex quinquefasciatus, aedes (stegomyia) aegypti and aedes (stegomyia) albopictus experimentally infected with d. immitis.201830018997
the use of insecticide-treated curtains for control of aedes aegypti and dengue virus transmission in "fraccionamiento" style houses in méxico.dengue, chikungunya, yellow fever, and zika viruses transmitted by aedes aegypti mosquitoes are major public health threats in the tropical and subtropical world. in méxico, construction of large tracts of "fraccionamientos" high density housing to accommodate population growth and urbanization has provided fertile ground for ae. aegypti-transmitted viruses. we investigated the utility of pyrethroid-treated window curtains to reduce both the abundance of ae. aegypti and to prevent dengue virus ( ...201830018645
zika virus outbreak in the pacific: vector competence of regional vectors.in 2013, zika virus (zikv) emerged in french polynesia and spread through the pacific region between 2013 and 2017. several potential aedes mosquitoes may have contributed to the zikv transmission including aedes aegypti, the main arbovirus vector in the region, and aedes polynesiensis, vector of lymphatic filariasis and secondary vector of dengue virus. the aim of this study was to analyze the ability of these two pacific vectors to transmit zikv at a regional scale, through the evaluation and ...201830016372
molecular characterization of the cry gene profile of bacillus thuringiensis isolated from a caribbean region of colombia.in order to characterize native strains of bacillus thuringiensis of the colombian caribbean with toxic effect against insect vectors, 28 samples of bacteria identified as b. thuringiensis were isolated from different soils and muds around the city of valledupar. using a biological test, five isolates of b. thuringiensis showed toxic effect against larvae of aedes aegypti. pcr methods were used to detect cry1, cry2, cry4b, cry10 and cyt1 genes. cry1 and cry2 genes were detected in 35.7% and 32.1 ...201830015421
evidence of vertical transmission of zika virus in field-collected eggs of aedes aegypti in the brazilian amazon.arboviruses are viruses transmitted to humans and other animals by the bite of hematophagous arthropods. infections caused by chikungunya virus (chikv), dengue virus (denv), zika virus (zikv), and the deadlier yellow fever virus (yfv) are current public health problems in several countries, mainly those located in tropical and subtropical regions. one of the main prevention strategies continues to be vector control, with the elimination of breeding sites and surveillance of infested areas. the u ...201830011278
transmission potential of mayaro virus in florida aedes aegypti and aedes albopictus mosquitoes.mayaro virus (mayv) is an emerging mosquito-borne arbovirus present in central and south america that causes arthralgia and febrile illness. domestic mosquitoes aedes aegypti (diptera: culicidae) and aedes albopictus are potential vectors of mayv that may allow for transmission to humans in urban settings. the present paper assesses susceptibility to infection, disseminated infection and transmission potential in florida ae. aegypti and ae. albopictus for mayv. oral infection was significantly h ...201830006976
the occurrence, diversity and blood feeding patterns of potential vectors of dengue and yellow fever in kacheliba, west pokot county, kenya.yellow fever (yf) and dengue (den) viruses are important re-emerging mosquito-borne viruses sharing similar vectors and reservoirs. the last documented yf outbreak in kenya occurred in 1992-95. however, yf virus is re-emerging in bordering countries including uganda, ethiopia and south sudan with the potential for spread to the neighboring regions in kenya. dengue is endemic in kenya with outbreaks being detected in various towns in the north and the coast. this study reports on the aedes (stego ...201830006028
synergy in the adulticidal efficacy of essential oils for the improvement of permethrin toxicity against aedes aegypti l. (diptera: culicidae).in a previous screening program for mosquitocides from local edible plants in thailand, essential oils (eos) of cyperus rotundus, alpinia galanga and cinnamomum verum, were found to possess promising adulticidal activity against aedes aegypti. with the aim of reducing usage of conventional insecticides and improving the management of resistant mosquito populations, this study was designed to determine the potential synergism in the adulticidal efficacy of eos on permethrin toxicity against ae. a ...201830005688
epidemiological significance of dengue virus genetic variation in mosquito infection dynamics.the kinetics of arthropod-borne virus (arbovirus) transmission by their vectors have long been recognized as a powerful determinant of arbovirus epidemiology. the time interval between virus acquisition and transmission by the vector, termed extrinsic incubation period (eip), combines with vector mortality rate and vector competence to determine the proportion of infected vectors that eventually become infectious. however, the dynamic nature of this process, and the amount of natural variation i ...201830005085
the c-type lectin domain gene family in aedes aegypti and their role in arbovirus infection.several medically important flaviviruses that are transmitted by mosquitoes have been shown to bind to the c-type lectin fold that is present in either vertebrate or invertebrate proteins. while in some cases this interaction is part of a neutralizing anti-viral immune response, many reports have implicated this as critical for successful virus entry. despite the establishment of mosquito c-type lectin domain containing proteins (ctldcps) as known host factors in assisting the infectious process ...201830002303
salinity tolerant aedes aegypti and ae. albopictus-infection with dengue virus and contribution to dengue transmission in a coastal peninsula.aedes aegypti and ae. albopictus are major arboviral vectors that are considered to lay eggs, and undergo preimaginal development only in fresh water collections. however, recently they have been also shown to develop in coastal brackish water habitats. the ability of the biologically variant salinity-tolerant aedes vectors to transmit arboviral diseases is unknown. we therefore, investigated the infection of salinity-tolerant aedes mosquitoes with dengue virus (denv) and analysed dengue inciden ...201829916445
screening of natural wolbachia infection in aedes aegypti, aedes taeniorhynchus and culex quinquefasciatus from guadeloupe (french west indies).guadeloupe islands are threatened by several mosquito-borne viruses such as dengue, chikungunya, zika and west nile virus. it appears essential to look for alternative mosquito control methods such as the incompatible insect technique (itt) aiming at sterilizing wild females by inundative releases of incompatible males. before considering the implementation of such a strategy, the characterization of genetic diversity of the endocellular bacterium wolbachia regarding the local mosquito populatio ...201829908171
prediction and prevention of urban arbovirus epidemics: a challenge for the global virology community.the recent emergence and rapid spread of zika virus in tropical regions of the western hemisphere took arbovirologists and public health officials by surprise, and the earlier transfers of west nile and chikungunya viruses from the old to the new world were also unexpected. these pandemics underscore the increasing threat of zoonotic arboviruses, especially those that are capable of entering into human-amplified, urban transmission cycles transmitted by aedes (stegomyia) aegypti and sometimes ot ...201829906475
lack of evidence for natural wolbachia infections in aedes aegypti (diptera: culicidae).wolbachia is a genus of endosymbiotic bacteria that infects 66% of all insect species. its major impact on insects is in reproduction: sterility, production of one sex, and/or parthenogenesis. another effect was discovered when the disease-transmitting mosquito, aedes aegypti linnaeus (diptera: culicidae), was infected with wolbachia isolated from drosophila: infected female mosquitoes became less capable of transmitting diseases such as dengue fever and chikungunya. this has led to releases of ...201829901734
restoration of pyrethroid susceptibility in a highly resistant aedes aegypti population.insecticide resistance has evolved in disease vectors worldwide, creating the urgent need to either develop new control methods or restore insecticide susceptibility to regain use of existing tools. here we show that phenotypic susceptibility can be restored in a highly resistant field-derived strain of aedes aegypti in only 10 generations through rearing them in the absence of insecticide.201829899128
signifying zika: heterogeneity in the representations of the virus by history of infection.despite having been broadly advertised by the mass media, many negative consequences of the zika virus have been less significant than originally predicted. it is likely that after a few months from the epidemic's onset, personal experience with the virus has altered the person's way to deal with the disease. this study explores the relation between exposure to zika virus and the social representation of the epidemic. more specifically, one analyzes if increased exposure to the risk of zika infe ...201829898004
evaluation of the effects of the larvicides temephos on reproductive performance, embryofetal development and dna integrity of swiss mice.temephos is considered the gold standard by the ministry of health for controlling the larvae of the mosquito aedes aegypti. the present study evaluated the effects of temephos larvicide on the reproductive performance, embryo-fetal development and dna integrity of swiss mice. this study used 30 pregnant female mice: 10 were controls treated with drinking water at a dosage of 0.1 ml/10 g (body weight - b.w., administered orally - a.o.), and 20 were treated with temephos at doses of 0.0043 mg/kg ...201829891373
what does the future hold for yellow fever virus? (i).the recent resurgence of yellow fever virus (yfv) activity in the tropical regions of africa and south america has sparked renewed interest in this infamous arboviral disease. yellow fever virus had been a human plague for centuries prior to the identification of its urban transmission vector, the aedes (stegomyia) aegypti (linnaeus) mosquito species, and the development of an efficient live-attenuated vaccine, the yf-17d strain. the combination of vector-control measures and vaccination campaig ...201829890711
a transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, aedes aegypti.populations of aedes aegypti naturally exhibit variable susceptibility to dengue viruses. this natural variation can be impacted by nutritional stress resulting from larval-stage crowding, indicating the influence of environment components on the adult mosquito immune response. in particular, larval crowding was previously shown to reduce the susceptibility of adult females of a trinidad field isolate of a. aegypti to the dengue serotype 2 (jam1409) virus. here, we present the first whole transc ...201829889847
target and non-target toxicity of fern extracts against mosquito vectors and beneficial aquatic organisms.dengue and malaria are significant mosquito-borne diseases that are rapidly spread worldwide, mainly in temperate countries. pteridophytes were identified to be a significant source of novel mosquitocidal agents. the present research was to explore the eco-friendly larvicides from methanol extracts of ferns, viz., actiniopteris radiata, adiantum caudatum, cheilanthes swartzii, hemionitis arifolia and lycopodium clavatum. the larvicidal potential of the extracts screened using larvae of dengue ve ...201829885618
viral determinants and vector competence of zika virus transmission.zika virus (zikv) has emerged as a new global health threat. since its first discovery in zika forest in uganda, this virus has been isolated from several mosquito species, including aedes aegypti and aedes albopictus. the geographical distribution of these mosquito species across tropical and subtropical regions has led to several outbreaks, including the recent pandemic in brazil, followed by the pacific islands and other areas of north and south america. this has gained attention of the scien ...201829875751
multiscale approach to deciphering the molecular mechanisms involved in the direct and intergenerational effect of ibuprofen on mosquito aedes aegypti.the anti-inflammatory ibuprofen is a ubiquitous surface water contaminant. however, the chronic impact of this pharmaceutical on aquatic invertebrate populations remains poorly understood. in model insect aedes aegypti, we investigated the intergenerational consequences of parental chronic exposure to an environmentally relevant concentration of ibuprofen. while exposed individuals did not show any phenotypic changes, their progeny showed accelerated development and an increased tolerance to sta ...201829874051
effects of temephos, permethrin, and eucalyptus nitens essential oil on survival and swimming behavior of aedes aegypti and anopheles pseudopunctipennis (diptera: culicidae) larvae.an essential strategy to deal with mosquito-borne diseases is the control of larvae in their development sites. the mosquitoes anopheles pseudopunctipennis (theobald) (diptera: culicidae), a malaria vector, and aedes aegypti (l.) (diptera: culicidae), vector of dengue, zika, yellow fever, and chikungunya viruses, breed in very different habitats. insecticide treatments of mosquito larvae focus mainly on their lethal effects. however, insecticide degradation or the poor dosage of larvicides will ...201829873741
vertical transmission of dengue virus in the yogyakarta airport area.international health regulations controls international travel including human movement, disease vector, and imported items to prevent the spread of dengue, especially in seaports, airports, and border crossing posts. this study aimed to determine dengue transovarial transmission index (tti) and distribution of dengue virus in the areas around adisucipto airport of yogyakarta, indonesia.201829871615
differential effects of temperature and mosquito genetics determine transmissibility of arboviruses by aedes aegypti in argentina.aedes aegypti (l.) (diptera: culicidae) have a global distribution and are the primary vector of a number of mosquito-borne viruses responsible for epidemics throughout the americas. as in much of south america, the threat from pathogens including dengue virus (denv; flaviviridae, flavivirus) and chikungunya virus (chikv; togaviridae, alphavirus) has increased in argentina in recent years. the complexity of transmission cycles makes predicting the occurrence and intensity of arbovirus outbreaks ...201829869610
serosurvey of human antibodies recognizing aedes aegypti d7 salivary proteins in colombia.dengue is one of the most geographically significant mosquito-borne viral diseases transmitted by aedes mosquitoes. during blood feeding, mosquitoes deposit salivary proteins that induce antibody responses. these can be related to the intensity of exposure to bites. some mosquito salivary proteins, such as d7 proteins, are known as potent allergens. the antibody response to d7 proteins can be used as a marker to evaluate the risk of exposure and disease transmission and provide critical informat ...201829868532
effect of the topical repellent para-menthane-3,8-diol on blood feeding behavior and fecundity of the dengue virus vector aedes aegypti.dengue fever is an acute disease caused by the dengue virus and transmitted primarily by the mosquito aedes aegypti. the current strategy for dengue prevention is vector control including the use of topical repellents to reduce mosquito biting. although n,n-diethyl-m-methylbenzamide (deet) is the most common active ingredient in topical repellent products, para-menthane-3,8-diol (pmd) is also used commercially. studies have indicated pmd reduced biting by 90⁻95% for up to 6⁻8 h, similar to the e ...201829867036
pyrethroid resistance in the dengue vector aedes aegypti in southeast asia: present situation and prospects for management.human arboviral diseases transmitted by aedes aegypti such as dengue, zika, chikungunya and yellow fever remain global public health threats to date. of these diseases, dengue fever is particularly prevalent in southeast asia. relentless vector control efforts are performed to curtail disease transmissions through which pyrethroid insecticides are broadly used as the first line of defense to control ae. aegypti, especially in the course of disease outbreaks. here, we compile the largest contempo ...201829866193
molecular and functional characterization of single-box high-mobility group b (hmgb) chromosomal protein from aedes aegypti.high-mobility group b (hmgb) proteins have highly conserved, unique dna-binding domains, hmg boxes, that can bind non-b-type dna structures, such as bent, kinked and unwound structures, with high affinity. hmgb proteins also promote dna bending, looping and unwinding. in this study, we determined the role of the aedes aegypti single hmg-box domain protein aahmgb; characterized its structure, spatiotemporal expression levels, subcellular localization, and nucleic acid binding activities; and comp ...201829859286
technologies to combat aedes mosquitoes: a model based on smart city.aedes aegypti and aedes albopictus mosquitoes are responsible for the transmission of diseases such as dengue fever, yellow fever, chikungunya fever, zika virus fever, some of which can cause irreversible central nervous system problems and death. this study investigates what technologies are being used for combatting and monitoring the aedes mosquitoes and to propose joining these technologies into a single and complete solution using the smart cities concept. a search for newscasts on google a ...201829857404
exposure of a diurnal mosquito vector to floral mimics: foraging responses, feeding patterns, and significance for sugar bait technology.food location by mosquitoes is mediated by resource-derived olfactory and visual signals. smell sensation is intermittent and dependent on the environment, whereas visual signals are continual and precede olfactory cues. success of mosquito bait technology, where olfactory cues are used for attraction, is being impeded by reduced attractiveness. despite proof that mosquitoes respond to colored objects, including those mimicking floral shape, and that they can discriminate among flowers, the impa ...201829856985
the awed trial (applying wolbachia to eliminate dengue) to assess the efficacy of wolbachia-infected mosquito deployments to reduce dengue incidence in yogyakarta, indonesia: study protocol for a cluster randomised controlled trial.dengue and other arboviruses transmitted by aedes aegypti mosquitoes, including zika and chikungunya, present an increasing public health challenge in tropical regions. current vector control strategies have failed to curb disease transmission, but continue to be employed despite the absence of robust evidence for their effectiveness or optimal implementation. the world mosquito program has developed a novel approach to arbovirus control using ae. aegypti stably transfected with wolbachia bacter ...201829855331
a highly secure method for rearing aedes aegypti mosquitoes.vector-borne infectious diseases are caused by pathogenic microorganisms transmitted mainly by blood-sucking arthropod vectors. in laboratories, the handling of insects carrying human pathogens requires extra caution because of safety concerns over their escape risk. based on standard insect containment practices, there have been cases where costly enhancements were required to definitely protect laboratory workers and neighbors from potential infection through mosquito bites. here, we developed ...201829849477
dengue in singapore from 2004 to 2016: cyclical epidemic patterns dominated by serotypes 1 and 2.singapore has experienced periodic dengue epidemics despite maintaining a low aedes house index. each epidemic was associated with a switch in the predominant serotype. we investigated the temporal dynamics of dengue fever and dengue virus (denv) and analyzed the epidemiological and entomological patterns of dengue in singapore from 2004 to 2016. the case surveillance is based on a mandatory notification system that requires all medical practitioners to report clinically suspected and laboratory ...201829848407
report of chikungunya virus in wild populations of aedes aegypti in guerrero state, mexico.we detected vertical transmission of chikungunya virus (chikv) in wild populations of aedes aegypti from san marcos, guerrero, mexico, with real-time reverse transcriptase-polymerase chain reaction. a total of 20 pools (1-11 specimens/pool) of larvae, male, and female mosquitoes were tested. we report the detection of chikv in 2 of 11 larval pools, 4 of 5 male pools, and 1 of 4 female pools, from field-collected mosquitoes.201831442150
a survey of the mosquito species in maxcanu, yucatan, mexico.a survey was carried out to identify the mosquitoes inhabiting human premises in the rural locality of maxcanú, yucatán, mexico. using the centers for disease control and prevention backpack aspirators, simple random sampling was carried out inside of 101 homes during the november 2013 rainy season. a total of 1,492 specimens were collected. three subfamilies (anophelinae, culicinae, and toxorhynchitinae) and 5 species were identified: anopheles albimanus, aedes aegypti, culex interrogator, lima ...201831442149
a survey of the mosquitoes of kosrae state, federated states of micronesia, 2016.in response to an outbreak of zika virus that started in february 2016 on kosrae island, kosrae state, federated states of micronesia, we conducted entomological investigations, including a survey to characterize the mosquito fauna on kosrae, from november 29 to december 8, 2016. mosquitoes were collected using several surveillance methods in order to sample all stages of the mosquito life cycle. eggs were collected using ovicups, larvae and pupae were sampled using standard dippers, and adults ...201831414080
[knowledge and practices in aedes aegypti control among different social subjects in salvador, bahia state, brazil].due to the persistence of dengue and other arbovirus infections in brazil, the government has stepped up measures to combat the aedes aegypti mosquito vector. the responsibilities of community endemic disease workers (cedw) and community health workers (chw) include acting as intermediaries and disseminating knowledge in the community. the aim of this study was to analyze knowledge and practices in dengue control by different social subjects: residents and cedw/chw. interviews were held with res ...201829846399
follow up of a robust meta-signature to identify zika virus infection in aedes aegypti: another brick in the wall.the mosquito aedes aegypti is the main vector of several arthropod-borne diseases that have global impacts. in a previous meta-analysis, our group identified a vector gene set containing 110 genes strongly associated with infections of dengue, west nile and yellow fever viruses. of these 110 genes, four genes allowed a highly accurate classification of infected status. more recently, a new study of ae. aegypti infected with zika virus (zikv) was published, providing new data to investigate wheth ...201829846381
the use of wolbachia by the world mosquito program to interrupt transmission of aedes aegypti transmitted viruses.the biological control of mosquito transmission by the use of the naturally occurring insect-specific bacterial endosymbiont wolbachia has been successfully tested in small field trials. the approach has been translated successfully to larger field sites in townsville, australia and expanded to more than 10 countries through the eliminate dengue program. the broader application of the program beyond limiting the transmission of dengue and including other aedes aegypti borne mosquitoes has seen t ...201829845544
structures of zika virus e & ns1: relations with virus infection and host immune responses.zika virus (zikv), first discovered in the zika forest in uganda in 1947 was understudied until the recent explosive epidemic in several south american countries where it has become strongly associated with congenital birth defects leading to severe cranial malformations and neurological conditions. the increase in number of case of microcephaly in newborn children associated with zikv infection triggered the world health organization to declare the epidemic as a public health emergency of inter ...201829845526
back-to-the-future potential for autochthonous transmission of aedes aegypti-transmitted viruses in ethekwini and urban coastal kwazulu-natal province, south africa. 201829843848
the risk of urban yellow fever resurgence in aedes-infested american cities.aedes aegypti, historically known as yellow fever (yf) mosquito, transmits a great number of other viruses such as dengue, west nile, chikungunya, zika, mayaro and perhaps oropouche, among others. well established in africa and asia, aedes mosquitoes are now increasingly invading large parts of the american continent, and hence the risk of urban yf resurgence in the american cities should because of great concern to public health authorities. although no new urban cycle of yf was reported in the ...201829843824
evaluation of larvicidal, adulticidal, and anticholinesterase activities of essential oils of illicium verum hook. f., pimenta dioica (l.) merr., and myristica fragrans houtt. against zika virus vectors.aedes aegypti is the vector responsible for transmitting pathogens that cause various infectious diseases, such as dengue, zika, yellow fever, and chikungunya, worrying health authorities in the tropics. due to resistance of mosquitoes to synthetic insecticides, the search for more effective insecticidal agents becomes crucial. the aim of this study was to verify the larvicidal, adulticidal, and anticholinesterase activities of the essential oils of the illicium verum (eoiv), pimenta dioica (eop ...201829808407
rapid, noninvasive detection of zika virus in aedes aegypti mosquitoes by near-infrared spectroscopy.the accelerating global spread of arboviruses, such as zika virus (zikv), highlights the need for more proactive mosquito surveillance. however, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. we show for the first time that near-infrared spectroscopy (nirs) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect zikv in heads and thoraces of intact aedes aegypti mosquitoes with ...201829806030
impact of short-term temperature challenges on the larvicidal activities of the entomopathogenic watermold leptolegnia chapmanii against aedes aegypti, and development on infected dead larvae.the oomycete leptolegnia chapmanii is among the most promising entomopathogens for biological control of aedes aegypti. this mosquito vector breeds in small water collections, where this aquatic watermold pathogen can face short-term scenarios of challenging high or low temperatures during changing ambient conditions, but it is yet not well understood how extreme temperatures might affect the virulence and recycling capacities of this pathogen. we tested the effect of short-term exposure of ency ...201829801786
vapor toxicity of five volatile pyrethroids against aedes aegypti, aedes albopictus, culex quinquefasciatus, and anopheles quadrimaculatus (diptera: culicidae).mosquito mortality has been documented in numerous studies of spatial repellents but the concentration-dependent toxicity of spatial repellent vapors has not been documented. to address this issue, prallethrin, flumethrin, metofluthrin, transfluthrin, and meperfluthrin were selected for comparative study against aedes albopictus (skuse), ae. aegypti (l.), culex quinquefasciatus say, and anopheles quadrimaculatus say. mosquito were exposed to vapors of each chemical for 2, 4, and 24 h with mortal ...201829797795
codon adaptation biases among sylvatic and urban genotypes of dengue virus type 2.dengue virus (denv) emerged from the sylvatic environment and colonized urban settings, being sustained in a human-aedes-human transmission chain, mainly by the bites of females of the anthropophilic species aedes aegypti. herein, we sought evidence for fine-tuning in viral codon usage, possibly due to viral adaptation to human transmission. we compared the codon adaptation of denv serotype 2 (denv-2) genotypes from urban and sylvatic habitats and tried to correlate the findings with key evoluti ...201829792991
surveillance of mosquitoes (diptera: culicidae) in southern iowa, 2016.the mosquito fauna of iowa has been extensively investigated over several decades, providing a wealth of information regarding species distributions, relative abundance, temporal activity patterns, and identifying vectors of medical importance. however, these investigations have had unequal coverage, leaving the mosquito fauna in some parts of the state, including southern iowa, largely uncharacterized. with the heightened public health threat of zika virus in the summer of 2016, greater emphasi ...201829788488
threshold dynamics of a temperature-dependent stage-structured mosquito population model with nested delays.mosquito-borne diseases remain a significant threat to public health and economics. since mosquitoes are quite sensitive to temperature, global warming may not only worsen the disease transmission case in current endemic areas but also facilitate mosquito population together with pathogens to establish in new regions. therefore, understanding mosquito population dynamics under the impact of temperature is considerably important for making disease control policies. in this paper, we develop a sta ...201829785519
a multi-faceted pandemic: a review of the state of knowledge on the zika virus.while until recently the small and isolated zika outbreaks in eastern asia and pacific islands had been overlooked, the large-scale outbreak that started in brazil in 2015 and the increase of microcephaly cases in the same place and time made media headlines. considered as harmless until recently, zika has given rise to an important global crisis that poses not only health challenges but also environmental, economical, social, and ethical challenges for states and people around the world. the ma ...201829785319
the effect of nonrandom mating on wolbachia dynamics: implications for population replacement and sterile releases in aedes mosquitoes.wolbachia bacteria are known to cause deviations from random mating and affect sperm competition (sc) in some of their arthropod hosts. because these effects could influence the effectiveness of wolbachia in mosquito population replacement and suppression programs, we developed a theoretical framework to investigate them and we collected relevant data for the wmel infection in aedes aegypti. using incompatibility patterns as a measure of mating success of infected versus uninfected mosquitoes, w ...201829968550
strain-specific pathogenicity and subversion of phenoloxidase activity in the mosquito aedes aegypti by members of the fungal entomopathogenic genus isaria.development of alternative vector control strategies are becoming more pressing given the rapid evolution of insecticide resistance and the rise of vector borne pathogens affecting public health such as dengue, chikungunya and zika. fungal-based biopesticides are promising alternatives to synthetic insecticides because they are ecofriendly and are highly effective at infecting insects through contact. this study evaluated the susceptibility of the yellow fever mosquito ae. aegypti to a range of ...201829967469
continued susceptibility of the wmel wolbachia infection in aedes aegypti to heat stress following field deployment and selection.aedes aegypti mosquitoes infected with the wmel strain of wolbachia are being deployed to control the spread of arboviruses around the world through blockage of viral transmission. blockage by wolbachia in some scenarios may be affected by the susceptibility of wmel to cyclical heat stress during mosquito larval development. we therefore evaluated the potential to generate a heat-resistant strain of wmel in ae. aegypti through artificial laboratory selection and through exposure to field tempera ...201829966368
variation in competence for zikv transmission by aedes aegypti and aedes albopictus in mexico.zikv is a new addition to the arboviruses circulating in the new world, with more than 1 million cases since its introduction in 2015. a growing number of studies have reported vector competence (vc) of aedes mosquitoes from several areas of the world for zikv transmission. some studies have used new world mosquitoes from disparate regions and concluded that these have a variable but relatively low competence for the asian lineage of zikv.201829965958
chikungunya in children: a clinical review.chikungunya (chikv) is an emerging arboviral infection with recent spikes in transmission in the americas. chikungunya is most commonly transmitted by mosquitos, specifically aedes aegypti and aedes albopictus. these mosquitoes are found throughout many parts of the united states. the classic tetrad of symptoms for chikv is fever, symmetric polyarthralgia, maculopapular rash, and nonpurulent conjunctivitis. although the majority (3 of 4) of infected people will be symptomatic, the viral illness ...201829965819
adventitious viruses persistently infect three commonly used mosquito cell lines.mosquito cell lines have been used extensively in research to isolate and propagate arthropod-borne viruses and understand virus-vector interactions. despite their utility as an in vitro tool, these cell lines are poorly defined and may harbor insect-specific viruses. accordingly, we screened four commonly-used mosquito cell lines, c6/36 and u4.4 cells from aedes albopictus, aag2 cells from aedes aegypti, and hsu cells from culex quinquefasciatus, for the presence of adventitious (i.e. exogenous ...201829957338
patterns of occurrence of dengue and chikungunya, and spatial distribution of mosquito vector aedes albopictus in swabi district, pakistan.two principal vector species, aedes aegypti and aedes albopictus, are known for transmission of dengue (den) and chikungunya (chk) in pakistan. we aimed to investigate their spatial and temporal distribution.201829956428
empirical optimization of risk thresholds for dengue: an approach towards entomological management of aedes mosquitoes based on larval indices in the kandy district of sri lanka.larval indices such as premise index (pi), breteau index (bi) and container index (ci) are widely used to interpret the density of dengue vectors in surveillance programmes. these indices may be useful for forecasting disease outbreaks in an area. however, use of the values of these indices as alarm signals is rarely considered in control programmes. therefore, the current study aims to propose threshold values for vector indices based on an empirical modeling approach for the kandy district of ...201829954443
comparison of chikungunya viruses generated using infectious clone or the infectious subgenomic amplicons (isa) method in aedes mosquitoes.reverse genetics systems provide the opportunity to manipulate viral genomes and have been widely used to study rna viruses and to develop new antiviral compounds and vaccine strategies. the recently described method called isa (infectious subgenomic amplicons) gives the possibility to rescue rna viruses in days. we demonstrated in cell culture that the use of the isa method led to a higher genetic diversity of viral populations than that observed using infectious clone technology. however, no r ...201829953474
aedes anphevirus: an insect-specific virus distributed worldwide in aedes aegypti mosquitoes that has complex interplays with wolbachia and dengue virus infection in cells.insect-specific viruses (isvs) of the yellow fever mosquito aedes aegypti have been demonstrated to modulate transmission of arboviruses such as dengue virus (denv) and west nile virus by the mosquito. the diversity and composition of the virome of a. aegypti, however, remains poorly understood. in this study, we characterized aedes anphevirus (aeav), a negative-sense rna virus from the order mononegavirales aeav identified from aedes cell lines was infectious to both a. aegypti and aedes albopi ...201829950416
arthropod evs mediate dengue virus transmission through interaction with a tetraspanin domain containing glycoprotein tsp29fb.dengue virus (denv) is a mosquito-borne flavivirus that causes dengue fever in humans, worldwide. using in vitro cell lines derived from aedes albopictus and aedes aegypti, the primary vectors of denv, we report that denv2/denv3-infected cells secrete extracellular vesicles (evs), including exosomes, containing infectious viral rna and proteins. a full-length denv2 genome, detected in arthropod evs, was infectious to naïve mosquito and mammalian cells, including human-skin keratinocytes and bloo ...201829946031
model-based analysis of experimental data from interconnected, row-configured huts elucidates multifaceted effects of a volatile chemical on aedes aegypti mosquitoes.insecticides used against aedes aegypti and other disease vectors can elicit a multitude of dose-dependent effects on behavioral and bionomic traits. estimating the potential epidemiological impact of a product requires thorough understanding of these effects and their interplay at different dosages. volatile spatial repellent (sr) products come with an additional layer of complexity due to the potential for altered movement of affected mosquitoes and diffusion of volatile particles of the produ ...201829941038
wolbachia enhances insect-specific flavivirus infection in aedes aegypti mosquitoes.mosquitoes transmit a diverse group of human flaviviruses including west nile, dengue, yellow fever, and zika viruses. mosquitoes are also naturally infected with insect-specific flaviviruses (isfs), a subgroup of the family not capable of infecting vertebrates. although isfs are not medically important, they are capable of altering the mosquito's susceptibility to flaviviruses and may alter host fitness. wolbachia is an endosymbiotic bacterium of insects that when present in mosquitoes limits t ...201829938064
using barcoded zika virus to assess virus population structure in vitro and in aedes aegypti mosquitoes.arboviruses such as zika virus (zikv, flaviviridae; flavivirus) must replicate in both mammalian and insect hosts possessing strong immune defenses. accordingly, transmission between and replication within hosts involves genetic bottlenecks, during which viral population size and genetic diversity may be significantly reduced. to help quantify these bottlenecks and their effects, we constructed 4 "barcoded" zikv populations that theoretically contain thousands of barcodes each. after identifying ...201829935423
circulation of chikungunya virus in aedes aegypti in maranhão, northeast brazil.the simultaneous circulation of dengue virus (denv), chikungunya virus (chikv) and zika virus (zikv) arboviruses have placed brazil among the main worldwide endemic areas. brazilian northeast region concentrates the highest incidence of infections caused by chikv and zikv. in maranhão, the second biggest northeastern state, there are cases of human infections caused by these three arboviruses and presence of aedes aegypti and aedes albopictus vectors. in this context, this study aimed to investi ...201829932932
ancient treatment for lice: a source of suggestions for carriers of other infectious diseases?louse infestation is one of the oldest contagious pestilential diseases of humankind, which has recently re-emerged in several developing countries as well as in homeless individuals and migrants. the present work provides the first phase of an historical excursus of louse remedies based on the classics of pharmaceutical literature, codes, pharmacopoeia and treatises. the second phase involves a literature search, based on the principal medical databases (scifinder, pubmed, google scholar, isi-w ...201829932096
evaluation of aedes aegypti (diptera: culicidae) life table attributes upon chikungunya virus replication reveals impact on egg-laying pathways.arthropod-borne virus disease cycles constitute interactions among three primary players-the host, the vector, and the virus-in which the virus needs to interact with the host and the vector to establish its survival. while in the host, some arboviruses replicate aggressively, resulting in host pathogenicity, and manifest as a disease condition. these viruses more often utilize the vectors as reservoirs before they are transmitted to the host and therefore do not amplify to as large titers as th ...201829931258
a high-risk zika and dengue transmission hub: virus detections in mosquitoes at a brazilian university campus.zika virus (zikv) and dengue virus (denv) are mosquito-borne flaviviruses prevalent throughout tropical regions. currently, management of zikv and denv centers on control of the primary vector aedes aegypti. this vector is highly anthropophilic and is therefore prevalent throughout densely urbanised landscapes. a new passive trap for gravid ae. aegypti (gravid aedes trap - gat) was developed for mosquito surveillance. here the different killing agents and the level of transmission of arboviruses ...201829929561
risk factors for the presence of dengue vector mosquitoes, and determinants of their prevalence and larval site selection in dhaka, bangladesh.dengue viruses are responsible for over 100 million infections a year worldwide and are a public health concern in bangladesh. although risk of transmission is high, data on vector population characteristics are scanty in bangladesh; therefore, a comprehensive prediction of the patterns of local virus transmission is not possible. recognizing these gaps, multi-year entomological surveys were carried out in dhaka, where the disease is most frequently reported. the specific objectives of the prese ...201829928055
vector competence of aedes aegypti, culex tarsalis, and culex quinquefasciatus from california for zika virus.zika virus (zikv) has emerged since 2013 as a significant global human health threat following outbreaks in the pacific islands and rapid spread throughout south and central america. severe congenital and neurological sequelae have been linked to zikv infections. assessing the ability of common mosquito species to transmit zikv and characterizing variation in mosquito transmission of different zikv strains is important for estimating regional outbreak potential and for prioritizing local mosquit ...201829927940
hydrophobic nanoprecipitates of β-cyclodextrin/avermectins inclusion compounds reveal insecticide activity against aedes aegypti larvae and low toxicity against fibroblasts.in the present work, hydrophobic nanoprecipitates (hnps) of inclusion complexes formed between β-cyclodextrin (βcd) and the avermectins (avms) named eprinomectin (epri) and ivermectin (iver) were synthesized and characterized, and their larvicidal activity against aedes aegypti and human safety against fibroblasts were evaluated. initially, thermogravimetric analysis/differential thermal analysis data revealed that inclusion increased the thermal stability of avms in the presence of βcd. nuclear ...201829925239
field testing & evaluation of the efficacy & duration of effectiveness of a biolarvicide, bactivec® sc (bacillus thuringiensis var. israelensis sh-14) in bengaluru, india.different formulations of bacillus thuringiensis var. israelensis (bti) have been tested against different mosquito vectors and other insects for their residual activity. in the present study, the efficacy and residual activity of a new formulation of bti (bactivec suspension concentrate) were evaluated against immature stages of anopheles stephensi liston (diptera: culicidae), aedes aegypti linnaeus (diptera: culicidae) and culex quinquefasciatus say (diptera: culicidae), in natural habitats in ...201829923520
identification of aedes aegypti cis-regulatory elements that promote gene expression in olfactory receptor neurons of distantly related dipteran insects.sophisticated tools for manipulation of gene expression in select neurons, including neurons that regulate sexually dimorphic behaviors, are increasingly available for analysis of genetic model organisms. however, we lack comparable genetic tools for analysis of non-model organisms, including aedes aegypti, a vector mosquito which displays sexually dimorphic behaviors that contribute to pathogen transmission. formaldehyde-assisted isolation of regulatory elements followed by sequencing (faire-se ...201829996889
tropomyosins in mosquito and house dust mite cross-react at the humoral and cellular level.aedes aegypti and dermatophagoides pteronyssinus contain important allergens including cross-reactive tropomyosins. however, the functional and clinical relevance of their cross-reactivity is still debated.201829992665
mosquito arbovirus survey in selected areas of kenya: detection of insect-specific virus.many arboviral outbreaks have occurred in various locations in kenya. entomological surveys are suitable methods for revealing information about circulating arboviruses before human outbreaks are recognized. therefore, mosquitoes were collected in kenya to determine the distribution of arboviruses.201829991925
environmentally-friendly green approach for the production of zinc oxide nanoparticles and their anti-fungal, ovicidal, and larvicidal properties.green synthesis of nanoparticles can be an important alternative compared to conventional physio-chemical synthesis. we utilized scadoxus multiflorus leaf powder aqueous extract as a capping and stabilizing agent for the synthesis of pure zinc oxide nanoparticles (zno nps). further, the synthesized zno nps were subjected to various characterization techniques. transmission electron microscope (tem) analysis showed an irregular spherical shape, with an average particle size of 31 ± 2 nm. furtherm ...201829986435
molecular aspects of the dengue virus infection process: a review.at the present time, dengue is one of the most important arboviruses affecting man, becoming a serious global public health problem, especially in subtropical and tropical countries, where environmental conditions favor the development and proliferation of the mosquito aedes aegypti. dengue is caused by a type of flavivírus, which is an enveloped virus of spherical geometry. nowadays, it is one of the diseases with the highest incidence in brazil, reaching the population of all states, regardles ...201829984641
aedes aegypti in the black sea: recent introduction or ancient remnant?the yellow fever mosquito aedes aegypti transmits viral diseases that have plagued humans for centuries. its ancestral home are forests of africa and ~400-600 years ago it invaded the new world and later europe and asia, causing some of the largest epidemics in human history. the species was rarely detected in countries surrounding the mediterranean sea after the 1950s, but during the last 16 years it re-appeared in madeira, russia and in the eastern coast of the black sea. we genotyped ae. aegy ...201829980229
west nile virus and other mosquito-borne viruses present in eastern europe.eastern europe (ee) has been severely affected by mosquito-borne viruses (moboviruses). in this review, we summarize the epidemiology of moboviruses, with particular attention to west nile virus (wnv). the study of wnv human cases in ee between 2010 and 2016, revealed that the epidemiology of wnv in ee is complex with the combination of introduction of different wnv strains from lineages 1 and 2, and the establishment of endemic cycles. we found a positive correlation between the risk of wnv re- ...201829979950
both living bacteria and eukaryotes in the mosquito gut promote growth of larvae.we recently reported that larval stage aedes aegypti and several other species of mosquitoes grow when living bacteria are present in the gut but do not grow when living bacteria are absent. we further reported that living bacteria induce a hypoxia signal in the gut, which activates hypoxia-induced transcription factors and other processes larvae require for growth. in this study we assessed whether other types of organisms induce mosquito larvae to grow and asked if the density of non-living mi ...201829979680
variation in temephos resistance in field populations of aedes aegypti (diptera: culicidae) in the state of sergipe, northeast brazil.the organophosphate temephos was first used in brazil in the 1960s for the control of aedes aegypti. because of its extensive and longstanding use worldwide, selection for mosquito populations resistant to the chemical has been observed not only in different regions of brazil but also in several parts of the world. the objective of this study was to evaluate the susceptibility of ae. aegypti to the organophosphate temephos, a larvicide used in vector control activities in sergipe/northeast brazi ...201829972557
zika virus: - a review of the main aspects of this type of arbovirosis.the objective of this study was to integrate findings related to zika virus from the scientific literature. an integrative review of medline was conducted, and data search was performed using the health sciences descriptor zika virus and the following filters: full texts available; studies in humans; full articles; and publications in portuguese, french, english, and spanish. after application of the filters, followed by reading of the titles, abstracts, and full texts, 44 studies were included ...201829972554
the surveillance of chikungunya virus in a temperate climate: challenges and possible solutions from the experience of lazio region, italy.chikv has become an emerging public health concern in the temperate regions of the northern hemisphere as a consequenceof the expansion of the endemic areas of its vectors (mainly aedes aegypti and aedesalbopictus). in 2017, a new outbreak of chikv was detected in italy with three clusters of autochthonous transmission in the lazio region (central italy), in the cities of anzio, rome, and latina and a secondary cluster in the calabria region (south italy). given the climate characteristics of it ...201830223536
where vectors collide: the importance of mechanisms shaping the realized niche for modeling ranges of invasive aedes mosquitoes.the vector mosquitoes aedes aegypti (l.), native to africa, and aedes albopictus (skuse), native to asia, are widespread invasives whose spatial distributions frequently overlap. predictive models of their distributions are typically correlative rather than mechanistic, and based on only abiotic variables describing putative environmental requirements despite extensive evidence of competitive interactions leading to displacements. here we review putative roles of competition contributing to dist ...201830220875
differences in the replicative capacities of clinical isolates of dengue virus in c6/36 cells and in urban populations of aedes aegypti from colombia, south america.dengue, the most prevalent arboviral disease worldwide, is caused by any of the four dengue virus (denv) serotypes that co-circulate constantly in hyperendemic areas such as medellin (colombia), and these serotypes are transmitted by mosquitoes of the genus aedes. in this study, we evaluated the replicative capacity of strains isolated in medellin between 2003 and 2007 in c6/36 cells and in colonies of aedes aegypti collected during 2010-2011 from high or low-incidence areas within the same city ...201830165044
direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of wolbachia using a cellphone.manipulation of natural mosquito populations using the endosymbiotic bacteria wolbachia is being investigated as a novel strategy to reduce the burden of mosquito-borne viruses. to evaluate the efficacy of these interventions, it will be critical to determine wolbachia infection frequencies in aedes aegypti mosquito populations. however, current diagnostic tools are not well-suited to fit this need. morphological methods cannot identify wolbachia, immunoassays often suffer from low sensitivity a ...201830161131
regulation of midgut cell proliferation impacts aedes aegypti susceptibility to dengue virus.aedes aegypti is the vector of some of the most important vector-borne diseases like dengue, chikungunya, zika and yellow fever, affecting millions of people worldwide. the cellular processes that follow a blood meal in the mosquito midgut are directly associated with pathogen transmission. we studied the homeostatic response of the midgut against oxidative stress, as well as bacterial and dengue virus (denv) infections, focusing on the proliferative ability of the intestinal stem cells (isc). i ...201829782512
zika virus: report from the task force on tropical diseases by the world federation of societies of intensive and critical care medicine.zika fever is an acute infectious disease caused by the zika virus (zikv), an rna virus belonging to the genus flavivirus. zikv can be transmitted by bites from aedes aegypti and aedes albopictus species, sexual activity, blood transfusion and from mother to fetus during pregnancy and delivery. a total of 50 territories and countries in the americas had reported zikv infections. for those with symptoms, the presentation is similar to dengue fever with body aches, joint pain, fatigue, malaise, fe ...201829779827
genome sequence analysis of a novel bacillus thuringiensis strain blb406 active against aedes aegypti larvae, a novel potential bioinsecticide.blb406 is a novel isolate of bacillus thuringiensis with a larvicidal activity against aedes aegypti larvae. it displays original plasmidic and crystal protein patterns. the present work reported molecular and bioinformatic analyses for the genome sequence of blb406 using miseq illumina next-generation sequencing technology. the reads were assembled by velvet tool. using rast program and pgaap, the genome of blb406 strain was shown to contain 6297 genes corresponding to 5924 protein coding seque ...201829778876
chemical profile and pharmacological effects of the resin and essential oil from bursera slechtendalii: a medicinal "copal tree" of southern mexico.this study reports on the volatile profile and pharmacological activities of the essential oil and resin from bursera slechtendalii, an aromatic medicinal tree commercialized in southern mexico. the essential oil contained 25 volatiles including p-cymen-8-ol (25.4-35.7%), n-hexyl hexanoate (6.8-17.6%) and n-octyl hexanoate (7.6-14.8%), whereas the crystallized resin contained 14 volatiles, including n-octyl acetate (29.6-34.2%), limonene (15.8-18.7%) and n-octyl butyrate (12.6-17-6%). the inhala ...201829777752
modeling dengue vector population using remotely sensed data and machine learning.mosquitoes are vectors of many human diseases. in particular, aedes ægypti (linnaeus) is the main vector for chikungunya, dengue, and zika viruses in latin america and it represents a global threat. public health policies that aim at combating this vector require dependable and timely information, which is usually expensive to obtain with field campaigns. for this reason, several efforts have been done to use remote sensing due to its reduced cost. the present work includes the temporal modeling ...201829777650
use of bead-based serologic assay to evaluate chikungunya virus epidemic, haiti.the index case of chikungunya virus (chikv) in haiti was reported during early 2014; the vector, the pervasive aedes aegypti mosquito, promoted rapid spread throughout the country. during december 2014-february 2015, we collected blood samples from 4,438 persons at 154 sites (62 urban, 92 rural) throughout haiti and measured chikv igg by using a multiplex bead assay. overall chikv seroprevalence was 57.9%; differences between rural (mean 44.9%) and urban (mean 78.4%) areas were pronounced. logis ...201829774861
the influence of urban heat islands and socioeconomic factors on the spatial distribution of aedes aegypti larval habitats.we addressed the potential associations among the temporal and spatial distribution of larval habitats of aedes (stegomyia) aegypti, the presence of urban heat islands and socioeconomic factors. data on larval habitats were collected in santa bárbara d'oeste, são paulo, brazil, from 2004 to 2006, and spatial and temporal variations were analysed using a wavelet-based approach. we quantified urban heat islands by calculating surface temperatures using the results of wavelet analyses and grey leve ...201829772891
urban environmental clustering to assess the spatial dynamics of aedes aegypti breeding sites.the identification of aedes aegypti breeding hotspots in urban areas is crucial for the rational design of control strategies against this disease vector. remote sensing and geographic information systems offer valuable tools for mapping habitat suitability of a given area. however, predicting species occurrences by means of probability distribution maps based on transversal entomological surveys has limited utility for local authorities. the aim of the present study was to carefully examine the ...201829772886
encapsidated host factors in alphavirus particles influence midgut infection of aedes aegypti.transmission of mosquito-borne viruses requires the efficient infection of both a permissive vertebrate host and a competent mosquito vector. the infectivity of sindbis virus (sinv), the type species of the alphavirus genus, is influenced by both the original and new host cell. we have shown that infection of vertebrate cells by sinv, chikungunya virus (chikv), and ross river virus (rrv) produces two subpopulations of virus particles separable based on density. in contrast, a single population o ...201829772674
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