Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| isolation of a larvicidal compound from piper solmsianum c.dc. (piperaceae). | the aedes aegypti mosquito is one of the major vectors of arboviruses. these diseases have re-emerged and the insecticides used nowadays are toxic to mammals and environment and have only been effective in the short-term. in this context, natural products are an alternative. the genus piper has many active compounds against arthropods, including neolignans. the present study evaluated the larvicidal potential of the n-hexanic extract of piper solmsianum and eupomatenoid-6, identified by gc-ms an ... | 2017 | 28882070 |
| association between suspected zika virus disease during pregnancy and giving birth to a newborn with congenital microcephaly: a matched case-control study. | in early 2015, an outbreak of an acute exanthematous illness with dengue-like symptoms occurred in northeastern brazil. by the end of the same year, an unexpected increase in the number of cases of microcephaly was observed in the region. the microcephaly outbreak cause was unknown and rumors pointing to various potential causes arose. since we were unaware at the time if this scenario would attract the interest of the broader scientific community, due to the neglected regions associated and as ... | 2017 | 28877754 |
| microrna-277 targets insulin-like peptides 7 and 8 to control lipid metabolism and reproduction in aedes aegypti mosquitoes. | hematophagous female mosquitoes transmit numerous devastating human diseases, including malaria, dengue fever, zika virus, and others. because of their obligatory requirement of a vertebrate blood meal for reproduction, these mosquitoes need a lot of energy; therefore, understanding the molecular mechanisms linking metabolism and reproduction is of particular importance. lipids are the major energy store providing the fuel required for host seeking and reproduction. they are essential components ... | 2017 | 28874536 |
| updated reported distribution of aedes (stegomyia) aegypti and aedes (stegomyia) albopictus (diptera: culicidae) in the united states, 1995-2016. | aedes (stegomyia) aegypti (l.) and aedes (stegomyia) albopictus (skuse) are potential vectors of zika, dengue, and chikungunya viruses in the united states. a zika virus outbreak in florida in the summer of 2016, driven by ae. aegypti and resulting in > 200 locally acquired cases of human illness, underscored the need for up-to-date information on the geographic distribution of ae. aegypti and ae. albopictus in the united states. in early 2016, we conducted a survey and literature review to comp ... | 2017 | 28874014 |
| "we need people to collaborate together against this disease": a qualitative exploration of perceptions of dengue fever control in caregivers' of children under 5 years, in the peruvian amazon. | dengue fever presents a significant and growing burden of disease to endemic countries, where children are at particular risk. worldwide, no effective anti-viral treatment has been identified, thus vector control is key for disease prevention, particularly in peru where no vaccine is currently available. this qualitative study aimed to explore the perceptions of dengue control in caregivers' of children under 5 years in peru, to help direct future mosquito control programmes and strategy. | 2017 | 28873408 |
| fat body organ culture system in aedes aegypti, a vector of zika virus. | the insect fat body plays a central role in insect metabolism and nutrient storage, mirroring functions of the liver and fat tissue in vertebrates. insect fat body tissue is usually distributed throughout the insect body. however, it is often concentrated in the abdomen and attached to the abdominal body wall. the mosquito fat body is the sole source of yolk proteins, which are critical for egg production. therefore, the in vitro culture of mosquito fat body tissues represents an important syste ... | 2017 | 28872112 |
| rapid evolution of ovarian-biased genes in the yellow fever mosquito (aedes aegypti). | males and females exhibit highly dimorphic phenotypes, particularly in their gonads, which is believed to be driven largely by differential gene expression. typically, the protein sequences of genes upregulated in males, or male-biased genes, evolve rapidly as compared to female-biased and unbiased genes. to date, the specific study of gonad-biased genes remains uncommon in metazoans. here, we identified and studied a total of 2927, 2013, and 4449 coding sequences (cds) with ovary-biased, testis ... | 2017 | 28630112 |
| determinants of outbreaks of emerging infectious diseases : the case of chikungunya in indian ocean, 2004-2007. | emerging outbreaks of arboviroses are most often tied to the convergence of a set of factors which have physical and environmental, genetic and biological, ecological, social, political and economic origins. thirteen out of these factors are known as main determinants of emerging infectious disease outbreaks. this paper assesses the role of these factors from exploring the chikungunya outbreak which occurred in indian ocean in 2004-2007, as case study. chikungunya is an alphavirus borne and tran ... | 2017 | 28627853 |
| the genetic diversity and population structure of domestic aedes aegypti (diptera: culicidae) in yunnan province, southwestern china. | there was no record of aedes aegypti in yunnan province, china, until 2002, but this species is now continuously found in nine cities (or counties). until now, little was known about the genetic diversity and population structure of this invasive species. thus, a detailed understanding of the invasion strategies, colonisation and dispersal of this mosquito from a population genetics perspective is urgently needed for controlling and eliminating this disease vector. | 2017 | 28610594 |
| deltamethrin resistance in aedes aegypti results in treatment failure in merida, mexico. | the operational impact of deltamethrin resistance on the efficacy of indoor insecticide applications to control aedes aegypti was evaluated in merida, mexico. a randomized controlled trial quantified the efficacy of indoor residual spraying (irs) against adult ae. aegypti in houses treated with either deltamethrin (to which local ae. aegypti expressed a high degree of resistance) or bendiocarb (to which local ae. aegypti were fully susceptible) as compared to untreated control houses. all adult ... | 2017 | 28604781 |
| qsar models for predicting the toxicity of piperidine derivatives against aedes aegypti. | qsar models are proposed for predicting the toxicity of 33 piperidine derivatives against aedes aegypti. from 2d topological descriptors, calculated with the padel software, ordinary least squares multilinear regression (ols-mlr) treatment from the qsarins software and machine learning and related approaches including linear and radial support vector machine (svm), projection pursuit regression (ppr), radial basis function neural network (rbfnn), general regression neural network (grnn) and k-ne ... | 2017 | 28604113 |
| [new strategies for the eradication of aedes aegypti: what challenges do we face in latin america?] | not available. | 2017 | 28603242 |
| comparative efficiency of biogents gravid aedes trap, cdc autocidal gravid ovitrap, and cdc gravid trap in northeastern florida. | we conducted a study to compare the effectiveness of the biogents gravid aedes trap (bg-gat) and centers for disease control and prevention (cdc) autocidal gravid ovitrap (ago) with that of the cdc gravid trap (cdc-gt) (as a standard) for their proficiency to collect mosquitoes in an urban residential neighborhood in northeastern florida. aedes aegypti , ae. albopictus, and culex quinquefasciatus were collected from each trap, with the latter species being predominant. significantly more cx. qui ... | 2017 | 28590226 |
| comparison of mosquito abundance from biogents sentinel 2.0 traps with and without rain covers. | biogents sentinel (bgs) traps have rapidly become a standard for adult aedes aegypti surveillance. several investigators have found that trap collections can be damaged easily by heavy dew or rain entering the trap intake port. in addition, water entering the trap may temporarily stop the fan, thus reducing the collection potential of the trap. we evaluated the effectiveness of a rain cover designed to minimize damage to mosquito trap collections from bgs 2.0 traps in a residential backyard in j ... | 2017 | 28590218 |
| diagnostic doses of insecticides for adult aedes aegypti to assess insecticide resistance in cuba. | the objective of this study was to determine diagnostic doses (dds) of 5 insecticides for the rockefeller susceptible strain of aedes aegypti , using the centers for disease control and prevention (cdc) bottle bioassay as a tool for monitoring insecticide resistance in the cuban vector control program. the 30-min dd values determined in this study were 13.5 μg/ml, 6.5 μg/ml, 6 μg/ml, 90.0 μg/ml, and 15.0 μg/ml for cypermethrin, deltamethrin, lambda-cyhalothrin, chlorpyrifos, and propoxur, respec ... | 2017 | 28590216 |
| effects of low-powered rf sweep between 0.01-20 ghz on female aedes aegypti mosquitoes: a collective behaviour analysis. | there are many products claiming to be an electronic solution towards repelling mosquitoes. several reviews were published in debunking these claims. however, there is a lack of a systematic study on effects of electromagnetic (em) or more specifically, radio frequency (rf) waves against mosquitoes due to the conclusions made in those years. therefore, we attempt to establish a fundamental study on female aedes aegypti (linnaeus) mosquitoes by quantifying the collective behavior of the mosquitoe ... | 2017 | 28582398 |
| corrigendum to "functional and immunohistochemical characterization of cceae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors aedes aegypti and ae. albopictus" [insect biochem. mol. biol. 74 (july 2016) 61-67]. | 2017 | 28578775 | |
| detection and perception of generic host volatiles by mosquitoes: responses to co2 constrains host-seeking behaviour. | natural selection has favoured specialization in anthropophilic mosquito host choice, yet in the absence of human hosts, females feed on a selected range of vertebrates. for host recognition, we hypothesize that mosquitoes primarily rely on generic host volatiles. detection and perception of such compounds would provide the mosquito with a flexible, yet constrained, odour coding system that could delineate host preference. in this study, we show that the quintessential generic volatile for host- ... | 2017 | 28573028 |
| a potent larvicidal agent against aedes aegypti mosquito from cardanol. | cardanol is a constituent of cashew nut shell liquid that presents larvicidal activity against aedes aegypti. the isolation of cardanol is somewhat troublesome, however, in this work we describe an efficient and inexpensive method to obtain it as a pure material. the compound was used as starting material to make chemical transformation leading to saturated cardanol, epoxides and, halohydrins. these derivatives were tested for toxicity against aedes aegypti larvae. the results showed that iodohy ... | 2017 | 28562821 |
| a new in vitro bioassay system for the discovery and quantitative evaluation of mosquito repellents. | mosquitoes vector many pathogens that cause human diseases. repellents play a significant role in reducing the risk of these diseases by preventing mosquito bites. in this paper, we are reporting an ali and khan (a & k), large cage in vitro bioassay system that can effectively be used to measure repellency of compounds against mosquitoes. the system uses temperature as a landing and feeding stimulus, and collagen as a feeding substrate. the minimum effective dose (med) of deet (n, n-diethyl-meta ... | 2017 | 28541424 |
| rna-seq comparison of larval and adult malpighian tubules of the yellow fever mosquito aedes aegypti reveals life stage-specific changes in renal function. | introduction: the life history of aedes aegypti presents diverse challenges to its diuretic system. during the larval and pupal life stages mosquitoes are aquatic. with the emergence of the adult they become terrestrial. this shifts the organism within minutes from an aquatic environment to a terrestrial environment where dehydration has to be avoided. in addition, female mosquitoes take large blood meals, which present an entirely new set of challenges to salt and water homeostasis. methods: to ... | 2017 | 28536536 |
| introducción. el virus del zika (zikv) es un flavivirus con envoltura, transmitido a los seres humanos principalmente por el vector aedes aegypti. la infección por zikv se ha asociado con un gran neurotropismo y con efectos neuropáticos, como el síndrome de guillain-barré en el adulto y la microcefalia fetal y posnatal, así como con un síndrome de infección congénita similar al producido por el virus de la rubéola (rv).objetivo. comparar las estructuras moleculares de la proteína de envoltura e ... | 2017 | 28527274 | |
| quantitative structure-activity relationship study of amide mosquito repellents. | a quantitative structure-activity relationship (qsar) study on 43 amide repellents was carried out by the heuristic method in order to reveal the correlations between molecular parameters of these amides and their repellency against aedes aegypti. sketches and optimizations of molecular structures were achieved by the gaussian software package. generation and screening of molecular parameters were accomplished using codessa 2.7.10 software. the leave-one-out method was applied for the model vali ... | 2017 | 28521600 |
| heat in the southeastern united states: characteristics, trends, and potential health impact. | high summer temperatures in extratropical areas have an impact on the public's health, mainly through heat stress, high air pollution concentrations, and the transmission of tropical diseases. the purpose of this study is to examine the current characteristics of heat events and future projections of summer apparent temperature (at)-and associated health concerns-throughout the southeastern united states. synoptic climatology was used to assess the atmospheric characteristics of extreme heat day ... | 2017 | 28520817 |
| laboratory and semi-field evaluations of two (transfluthrin) spatial repellent devices against aedes aegypti (l.) (diptera: culicidae). | two transfluthrin-based spatial repellent products (raid dual action insect repellent and home freshener and raid shield (currently not commercially available), sc johnson, racine wi) were evaluated for spatial repellent effects against female aedes aegypti (l.) mosquitoes under laboratory (wind tunnel) and semi-field (outdoor enclosure) conditions. the placement of either product in the wind tunnel significantly reduced host-seeking behaviors. the mean baseline (control) landing counts for the ... | 2017 | 28511270 |
| reemergence of yellow fever in ethiopia after 50 years, 2013: epidemiological and entomological investigations. | yellow fever (yf) is a viral hemorrhagic disease transmitted by aedes mosquito species. approximately, 200,000 cases and 30,000 deaths occur worldwide every year. in ethiopia, the last outbreak was reported in 1966 with 2200 cases and 450 deaths. a number of cases with deaths from unknown febrile illness reported from south ari district starting from november 2012. this investigation was conducted to identify the causative agent, source of the outbreak and recommend appropriate interventions. | 2017 | 28506254 |
| aedes aegypti (l.) mosquitoes in trinidad, west indies: longevity case studies. | few laboratory and field studies have reported long survival periods for ae. aegypti females and even fewer have designed experiments to characterize this important life history trait. this study was conducted under laboratory conditions to determine the number of blood meals taken by individual females, the number of eggs laid per individual female, the length of the gonotrophic cycle, and the duration of female survival. the results showed individual females oviposited between 670 and 1,500 eg ... | 2017 | 28504438 |
| influence of shading and pedestrian traffic on the preference of aedes (stegomyia) aegypti (diptera: culicidae) for oviposition microenvironments. | aedes aegypti mosquitoes are highly adaptable to abiotic stimuli. to evaluate the influence of shading and pedestrian traffic on the preference of ae. aegypti for oviposition microenvironments, 20 sites were sampled weekly using ovitraps within the perimeter of universidade federal do espírito santo, located in são mateus, espírito santo, brazil. a spatial and statistical analysis was performed in order to assess the relationship between shading time, pedestrian traffic, and the presence of biol ... | 2017 | 28504433 |
| isolation of an insulin-like peptide from the asian malaria mosquito, anopheles stephensi, that acts as a steroidogenic gonadotropin across diverse mosquito taxa. | many insulin-like peptides (ilps) have been identified in insects, yet only a few were isolated in their native form for structural and functional studies. antiserum produced to ilp3 in aedes aegypti was used in a radioimmunoassay to monitor the purification of an ilp from heads of adult an. stephensi and recognized the ilp in other immunoassays. the structure of the purified peptide matched that predicted for the ilp3 in this species. the native form stimulated ecdysteroid production by ovaries ... | 2017 | 28502740 |
| metazoan ribosome inactivating protein encoding genes acquired by horizontal gene transfer. | ribosome inactivating proteins (rips) are rna n-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28s rrna. these enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. recently, we reported for the first time in silico evidence of rip encoding genes in metazoans, in two closely related species of insects: aedes aegypti and culex quinquefasciatus. here, we have experimentally confirmed the p ... | 2017 | 28500327 |
| larval interference competition between the native neotropical mosquito limatus durhamii and the invasive aedes aegypti improves the fitness of both species. | interspecific competition with native species during biological invasions can sometimes limit alien expansion. we aimed to determine the potential ecological effects of limatus durhamii theobald 1901, a native neotropical mosquito (diptera: culicidae) species, on the invasive species aedes (stegomyia) aegypti (linnaeus 1762) that breeds in the same artificial water containers. development time and adult dry mass were measured in 3 rearing conditions: control (a single larva), intraspecific compe ... | 2017 | 28497885 |
| assessing the effect of selection with deltamethrin on biological parameters and detoxifying enzymes in aedes aegypti (l.). | resistance to insecticides through one or several mechanisms has a cost for an insect in various parameters of its biological cycle. the present study evaluated the effect of deltamethrin on detoxifying enzymes and biological parameters in a population of aedes aegypti selected for 15 generations. the enzyme activities of alpha- and beta-esterases, mixed-function oxidases and glutathione-s-transferases were determined during selection, along with biological parameters. | 2017 | 28485040 |
| what does heat tell a mosquito? characterization of the orientation behaviour of aedes aegypti towards heat sources. | the use of heat as a cue for the orientation of haematophagous insects towards hot-blooded hosts has been acknowledged for many decades. in mosquitoes, thermoreception has been studied at the molecular, physiological and behavioural levels, and the response to heat has been evaluated in multimodal contexts. however, a direct characterization of how these insects evaluate thermal sources is still lacking. in this study we characterize aedes aegypti thermal orientation using a simple dual choice p ... | 2017 | 28479122 |
| eco-friendly synthesis of silver nanoparticles and its larvicidal property against fourth instar larvae of aedes aegypti. | in this study, larvicidal activity of silver nanoparticles (agnps) synthesised using apple extract against fourth instar larvae of aedes aegypti was determined. as a result, the agnps showed moderate larvicidal effects against ae. aegypti larvae (lc50 = 15.76 ppm and lc90 = 27.7 ppm). in addition, comparison of larvicidal activity performance of agnps at high concentration prepared using two different methods showed that ae. aegypti larvae was fully eliminated within the duration of 2.5 h. from ... | 2017 | 28476997 |
| photo-induced and phytomediated synthesis of silver nanoparticles using derris trifoliata leaf extract and its larvicidal activity against aedes aegypti. | due to the increased development of resistance of vectors against synthetic insecticides and chemical drugs, plant based insecticides serve as promising biocontrol agents for effective vector control. green approach for the synthesis of nanoparticles has been attained using environmentally safe, non-toxic plant extracts. the present study was aimed to investigate the potent larvicidal activity of silver nanoparticles (agnps) produced by derris trifoliata leaf extract in relation to the various c ... | 2017 | 28460330 |
| allergens involved in the cross-reactivity of aedes aegypti with other arthropods. | cross-reactivity between aedes aegypti and mites, cockroaches, and shrimp has been previously suggested, but the involved molecular components have not been fully described. | 2017 | 28434865 |
| n-aryl-n'-ethyleneaminothioureas effectively inhibit acetylcholinesterase 1 from disease-transmitting mosquitoes. | vector control of disease-transmitting mosquitoes by insecticides has a central role in reducing the number of parasitic- and viral infection cases. the currently used insecticides are efficient, but safety concerns and the development of insecticide-resistant mosquito strains warrant the search for alternative compound classes for vector control. here, we have designed and synthesized thiourea-based compounds as non-covalent inhibitors of acetylcholinesterase 1 (ache1) from the mosquitoes anoph ... | 2017 | 28433681 |
| identification of the septate junction protein gliotactin in the mosquito aedes aegypti: evidence for a role in increased paracellular permeability in larvae. | septate junctions (sjs) regulate paracellular permeability across invertebrate epithelia. however, little is known about the function of sj proteins in aquatic invertebrates. in this study, a role for the transmembrane sj protein gliotactin (gli) in the osmoregulatory strategies of larval mosquito (aedes aegypti) was examined. differences in gli transcript abundance were observed between the midgut, malpighian tubules, hindgut and anal papillae of a. aegypti, which are epithelia that participate ... | 2017 | 28432154 |
| allatotropin: a pleiotropic neuropeptide that elicits mosquito immune responses. | allatotropins (at) are neuropeptides with pleotropic functions on a variety of insect tissues. they affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. the present work provides experimental evidence that at elicits immune responses in two important mosquito disease vectors, anopheles albimanus and aedes aegypti. hemocytes and an immune-competent mosquito cell line responded to at by showing strong mor ... | 2017 | 28426765 |
| efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae). | the current zika health crisis in the americas has created an intense interest in mosquito control methods and products. mosquito vectors of zika are of the genus aedes, mainly the yellow fever mosquito, aedes aegypti. l. the use of repellents to alter mosquito host seeking behavior is an effective method for the prevention of mosquito-borne diseases. a large number of different spray-on repellents and wearable repellent devices are commercially available. the efficacies of many repellents are u ... | 2017 | 28423421 |
| habitat and density of oviposition opportunity influences aedes aegypti (diptera: culicidae) flight distance. | understanding the dispersal of aedes (aegypti (l.) diptera: culicidae) after consuming a potentially infectious bloodmeal is an important part of controlling the spread of the arboviruses it transmits. because of the impact on abundance, removal of oviposition sites is a key component of vector control. however, source reduction around a case may encourage dispersal of potentially infected vectors. we compare the effect of oviposition site availability on ae. aegypti dispersal behavior within 30 ... | 2017 | 28419273 |
| the impact of temperature and body size on fundamental flight tone variation in the mosquito vector aedes aegypti (diptera: culicidae): implications for acoustic lures. | aedes aegypti (l.) males use female flight tone as a means of mate localization. by playing the sound of a flying female, males can be attracted to a trap to monitor mosquito populations and the progress of transgenic male releases. however, the female flight tone used to attract males needs to be optimized to maximize trap effectiveness. the fundamental frequency of female flight tone could be influenced by both body size and ambient temperature. however, no analysis yet has considered both the ... | 2017 | 28402550 |
| immunohistochemical mapping and transcript expression of the gpa2/gpb5 receptor in tissues of the adult mosquito, aedes aegypti. | gpa2/gpb5 is a glycoprotein hormone found in most bilateral metazoans including the mosquito, aedes aegypti. to elucidate physiological roles and functions of gpa2/gpb5, we aim to identify prospective target tissues by examining the tissue- and sex-specific expression profile of its receptor, the leucine-rich repeat-containing g protein-coupled receptor 1 (lgr1) in the adult mosquito. western analyses using a heterologous system with cho-k1 cells, transiently expressing a. aegypti lgr1, yielded ... | 2017 | 28401307 |
| functional analysis of glycosylation of zika virus envelope protein. | zika virus (zikv) infection causes devastating congenital abnormities and guillain-barré syndrome. the zikv envelope (e) protein is responsible for viral entry and represents a major determinant for viral pathogenesis. like other flaviviruses, the zikv e protein is glycosylated at amino acid n154. to study the function of e glycosylation, we generated a recombinant n154q zikv that lacks the e glycosylation and analyzed the mutant virus in mammalian and mosquito hosts. in mouse models, the mutant ... | 2017 | 29091758 |
| darker eggs of mosquitoes resist more to dry conditions: melanin enhances serosal cuticle contribution in egg resistance to desiccation in aedes, anopheles and culex vectors. | mosquito vectors lay their white eggs in the aquatic milieu. during early embryogenesis water passes freely through the transparent eggshell, which at this moment is composed of exochorion and endochorion. within two hours the endochorion darkens via melanization but even so eggs shrink and perish if removed from moisture. however, during mid-embryogenesis, cells of the extraembryonic serosa secrete the serosal cuticle, localized right below the endochorion, becoming the third and innermost eggs ... | 2017 | 29084225 |
| assessing the population at risk of zika virus in asia - is the emergency really over? | on november 18, 2016, the who ended its designation of the zika virus (zikv) epidemic as a public health emergency of international concern (pheic). at the same time, zikv transmission continues in asia, with the number of asian countries reporting zika cases increasing over the last 2 years. applying a method that combines epidemiological theory with data on epidemic size and drivers of transmission, we characterised the population at risk of zikv infection from aedes aegypti mosquitoes in 15 c ... | 2017 | 29082009 |
| culex quinquefasciatus mosquitoes do not support replication of zika virus. | the rapid spread of zika virus (zikv) in the americas raised many questions about the role of culex quinquefasciatus mosquitoes in transmission, in addition to the key role played by the vector aedes aegypti. here we analysed the competence of cx. quinquefasciatus (with or without wolbachia endosymbionts) for a zikv isolate. we also examined the induction of rna interference pathways after viral challenge and the production of small virus-derived rnas. we did not observe any infection nor such s ... | 2017 | 29076805 |
| voltage-sensitive sodium channel mutations s989p + v1016g in aedes aegypti confer variable resistance to pyrethroids, ddt and oxadiazines. | aedes aegypti is a vector of several important human pathogens. control efforts rely primarily on pyrethroid insecticides for adult mosquito control, especially during disease outbreaks. a. aegypti has developed resistance nearly everywhere it occurs and insecticides are used. an important mechanism of resistance is due to mutations in the voltage-sensitive sodium channel (vssc) gene. two mutations, in particular, s989p + v1016g, commonly occur together in parts of asia. | 2017 | 29064635 |
| asymmetrical competition between aedes aegypti and culex quinquefasciatus (diptera: culicidae) coexisting in breeding sites. | aedes aegypti and culex quinquefasciatus are mosquito vectors for several tropical diseases that represent a current public health problem. the ecological requirements for each species are different, however, both species show high biological adaptability, which promotes their coexistence in the same breeding sites. the purpose of this study was to assess the effect of larval association between ae. aegypti and cx. quinquefasciatus under different laboratory conditions of food supply and tempera ... | 2017 | 29064390 |
| assessment of the insecticidal activity of afoxolaner against aedes aegypti in dogs treated with nexgard®. | twelve healthy dogs were studied in this parallel group, blinded, randomised, and negative controlled efficacy study. on day -1, the 12 dogs included were ranked within sex in descending order of individual pre-treatment (day -5) fed mosquito counts and randomly allocated by blocks of two dogs to the untreated control group or the afoxolaner-treated group. nexgard® (merial, now part of boehringer ingelheim animal health) was administered orally on day 0 in accordance with the european label inst ... | 2017 | 29063828 |
| synthesis and chemometrics of thymol and carvacrol derivatives as larvicides against aedes aegypti. | thymol and carvacrol have previously demonstrated larvicidal activity against aedes aegypti (diptera: culicidae). in view of this fact, it was of our interest to obtain synthetic derivatives and evaluate their larvicidal activity on ae. aegypti larvae. | 2017 | 29062856 |
| evaluation of larvicidal activity of essential oil from leaves of coccinia grandis against three mosquito species. | to study the chemical constituents and larvicidal activity of essential oil extracted from the leaves of coccinia grandis against three mosquito species. | 2017 | 29062847 |
| use of a recombinant mosquito densovirus as a gene delivery vector for the functional analysis of genes in mosquito larvae. | in vivo microinjection is the most commonly used gene transfer technique for analyzing the gene functions in individual mosquitoes. however, this method requires a more technically demanding operation and involves complicated procedures, especially when used in larvae due to their small size, relatively thin and fragile cuticle, and high mortality, which limit its application. in contrast, viral vectors for gene delivery have been developed to surmount extracellular and intracellular barriers. t ... | 2017 | 29053694 |
| comparative efficacy of commercial mosquito coils against aedes aegypti (diptera: culicidae) in malaysia: a nationwide report. | this study was conducted using the glass chamber method to determine the susceptibility status of the dengue vector, aedes aegypti (l.) from 11 states in malaysia to commercial mosquito coils containing four different active ingredients, namely metofluthrin, d-allethrin, d-trans allethrin, and prallethrin. aedes aegypti exhibited various knockdown rates, ranging from 14.44% to 100.00%, 0.00% to 61.67%, 0.00% to 90.00%, and 0.00% to 13.33% for metofluthrin, d-allethrin, d-trans allethrin, and pra ... | 2017 | 29048583 |
| evaluation of embryotoxic and embryostatic effects of the aqueous extract of rhizophora mangle and tannic acid on eggs and larvae of aedes aegypti. | rhizophora mangle is an abundant plant in mangroves and tannic acid is a polyphenol produced by the secondary metabolism of plants. the aim of the study was to evaluate the embryotoxic and embriostatic effects of the aqueous extract of r. mangle and synthetic tannic acid on eggs and larvae of aedes aegypti. a. aegypti eggs were exposed in duplicate at concentrations of 250, 500, 750 and 1000 µg/ml of extract and tannic acid for a period of 14 days. mineral water was used as a negative control. t ... | 2017 | 29044325 |
| characterization of the zika virus induced small rna response in aedes aegypti cells. | rna interference (rnai) controls arbovirus infections in mosquitoes. two different rnai pathways are involved in antiviral responses: the piwi-interacting rna (pirna) and exogenous short interfering rna (exo-sirna) pathways, which are characterized by the production of virus-derived small rnas of 25-29 and 21 nucleotides, respectively. the exo-sirna pathway is considered to be the key mosquito antiviral response mechanism. in aedes aegypti-derived cells, zika virus (zikv)-specific sirnas were pr ... | 2017 | 29040304 |
| patchy dna forms of the zika virus rna genome are generated following infection in mosquito cell cultures and in mosquitoes. | zika virus (zikv) is a mosquito-borne flavivirus and has historically been reported to cause mild symptomatic diseases during human infections. more recently, the explosion of microcephaly among infants born to zikv-infected women has made zikv a global public health concern. while zikv causes acute human diseases, infections of vector mosquitoes are basically non-pathogenic, allowing persistent infections and conferring lifelong ability to transmit the virus. recent studies have revealed that d ... | 2017 | 29039731 |
| lure-and-kill yeast interfering rna larvicides targeting neural genes in the human disease vector mosquito aedes aegypti. | new mosquito control strategies are vitally needed to address established arthropod-borne infectious diseases such as dengue and yellow fever and emerging diseases such as zika and chikungunya, all of which are transmitted by the disease vector mosquito aedes aegypti. in this investigation, saccharomyces cerevisiae (baker's yeast) was engineered to produce short hairpin rnas (shrnas) corresponding to the aedes aegypti orthologs of fasciculation and elongation protein zeta 2 (fez2) and leukocyte ... | 2017 | 29038510 |
| modeling the environmental suitability for aedes (stegomyia) aegypti and aedes (stegomyia) albopictus (diptera: culicidae) in the contiguous united states. | the mosquitoes aedes (stegomyia) aegypti (l.)(diptera:culicidae) and ae. (stegomyia) albopictus (skuse) (diptera:culicidae) transmit dengue, chikungunya, and zika viruses and represent a growing public health threat in parts of the united states where they are established. to complement existing mosquito presence records based on discontinuous, non-systematic surveillance efforts, we developed county-scale environmental suitability maps for both species using maximum entropy modeling to fit clim ... | 2017 | 29029153 |
| combined toxicity of three essential oils against aedes aegypti (diptera: culicidae) larvae. | essential oils are potential alternatives to synthetic insecticides because they have low mammalian toxicity, degrade rapidly in the environment, and possess complex mixtures of bioactive constituents with multi-modal activity against the target insect populations. twenty-one essential oils were initially screened for their toxicity against aedes aegypti (l.) larvae and three out of the seven most toxic essential oils (manuka, oregano, and clove bud essential oils) were examined for their chemic ... | 2017 | 29029151 |
| effect of climatic conditions and water bodies on population dynamics of the dengue vector, aedes aegypti (diptera: culicidae). | the population of mosquitoes is mainly influenced by the biotic and abiotic factors. although aedes aegypti was reported until 1960's in the punjab, pakistan, the population has increased dramatically since 2009 and caused severe epidemics in 2011 due to heavy floods and rains. thus, this study was carried out to know the effect of biotic and abiotic factors on the population of aedes aegypti. | 2017 | 29026852 |
| central nervous system effects of intrauterine zika virus infection: a pictorial review. | relatively few agents have been associated with congenital infections involving the brain. one such agent is the zika virus, which has caused several outbreaks worldwide and has spread in the americas since 2015. the zika virus is an arbovirus transmitted by infected female mosquito vectors, such as the aedes aegypti mosquito. this virus has been commonly associated with congenital infections of the central nervous system and has greatly increased the rates of microcephaly. ultrasonography (us) ... | 2017 | 29019762 |
| a multiplex pcr for detection of knockdown resistance mutations, v1016g and f1534c, in pyrethroid-resistant aedes aegypti. | mutation of the voltage-gated sodium channel (vgsc) gene, or knockdown resistance (kdr) gene, is an important resistance mechanism of the dengue vector aedes aegypti mosquitoes against pyrethroids. in many countries in asia, a valine to glycine substitution (v1016g) and a phenylalanine to cysteine substitution (f1534c) are common in ae. aegypti populations. the g1016 and c1534 allele frequencies have been increasing in recent years, and hence there is a need to have a simple and inexpensive tool ... | 2017 | 29017613 |
| temporal distribution and insecticide resistance profile of two major arbovirus vectors aedes aegypti and aedes albopictus in yaoundé, the capital city of cameroon. | aedes aegypti and ae. albopictus are the major epidemic vectors of several arbovirus diseases such as yellow fever, dengue, zika and chikungunya worldwide. both aedes vectors are presents in cameroon; however, knowledge on the dynamic of the distribution of these species across cities and their resistance profile to insecticide are limited. here, we assessed the current distribution of ae. aegypti and ae. albopictus in yaoundé, the capital city, established the resistance profile to insecticides ... | 2017 | 29017606 |
| hydrogen peroxide resistance in strigomonas culicis: effects on mitochondrial functionality and aedes aegypti interaction. | reactive oxygen species (ros) are toxic molecules involved in several biological processes such as cellular signaling, proliferation, differentiation and cell death. adaptations to oxidative environments are crucial for the success of the colonization of insects by protozoa. strigomonas culicis is a monoxenic trypanosomatid found in the midgut of mosquitoes and presenting a life cycle restricted to the epimastigote form. among s. culicis peculiarities, there is an endosymbiotic bacterium in the ... | 2017 | 28993269 |
| evaluation of gamma radiation-induced dna damage in aedes aegypti using the comet assay. | the study was undertaken to evaluate gamma radiation-induced dna damage in aedes aegypti. the comet assay was employed to demonstrate the extent of dna damage produced in adult male a. aegypti exposed to seven different doses of gamma radiation, ranging from 1 gy to 50 gy. dna damage was measured as the percentage of comet tail dna. a significant linear increase in dna damage was observed in all samples; the extent of damage being proportional to the dose of gamma radiation the organism received ... | 2017 | 28992792 |
| how does competition among wild type mosquitoes influence the performance of aedes aegypti and dissemination of wolbachia pipientis? | wolbachia has been deployed in several countries to reduce transmission of dengue, zika and chikungunya viruses. during releases, wolbachia-infected females are likely to lay their eggs in local available breeding sites, which might already be colonized by local aedes sp. mosquitoes. therefore, there is an urgent need to estimate the deleterious effects of intra and interspecific larval competition on mosquito life history traits, especially on the duration of larval development time, larval mor ... | 2017 | 28991902 |
| duplex real-time pcr assay distinguishes aedes aegypti from ae. albopictus (diptera: culicidae) using dna from sonicated first-instar larvae. | aedes aegypti (l.) and ae. albopictus (skuse) are important arbovirus vectors in the united states, and the recent emergence of zika virus disease as a public health concern in the americas has reinforced a need for tools to rapidly distinguish between these species in collections made by vector control agencies. we developed a duplex real-time pcr assay that detects both species and does not cross-amplify in any of the other seven aedes species tested. the lower limit of detection for our assay ... | 2017 | 28981691 |
| pyrethroid susceptibility has been maintained in the dengue vector, aedes aegypti (diptera: culicidae), in queensland, australia. | although pesticide resistance is common in insect vectors of human diseases, the evolution of resistance might be delayed if management practices are adopted that limit selection of resistance alleles. outbreaks of dengue fever have occurred in queensland, australia, since the late 1800s, leading to ongoing attempts to control the mosquito vector, aedes aegypti (l.). since the 1990s, pyrethroid insecticides have been used for this purpose, but have been applied in a strategic manner with a varie ... | 2017 | 28981684 |
| resistance status and resistance mechanisms in a strain of aedes aegypti (diptera: culicidae) from puerto rico. | puerto rico (pr) has a long history of vector-borne disease and insecticide-resistant aedes aegypti (l.). defining contributing mechanisms behind phenotypic resistance is critical for effective vector control intervention. however, previous studies from pr have each focused on only one mechanism of pyrethroid resistance. this study examines the contribution of p450-mediated enzymatic detoxification and sodium channel target site changes to the overall resistance phenotype of ae. aegypti collecte ... | 2017 | 28981681 |
| cutaneous manifestations of chikungunya fever: observations from an outbreak at a tertiary care hospital in southeast rajasthan, india. | chikungunya fever is caused by chikungunya virus which is transmitted by the bite of infected aedes aegypti and a. albopictus mosquitoes. | 2017 | 28979866 |
| infection rate of aedes aegypti mosquitoes with dengue virus depends on the interaction between temperature and mosquito genotype. | dengue fever is the most prevalent arthropod-transmitted viral disease worldwide, with endemic transmission restricted to tropical and subtropical regions of different temperature profiles. temperature is epidemiologically relevant because it affects dengue infection rates in aedes aegypti mosquitoes, the major vector of the dengue virus (denv). aedes aegypti populations are also known to vary in competence for different denv genotypes. we assessed the effects of mosquito and virus genotype on d ... | 2017 | 28978730 |
| identification of surveillance and control priority areas for dengue and other arboviruses transmitted by aedes aegypti in natal-rn, brazil: experience report. | to report the vigi@dengue experience, held in the municipality of natal-rn, brazil, from october/2015 to may/2016. | 2017 | 28977187 |
| circulation of denv2 and denv4 in aedes aegypti (diptera: culicidae) mosquitoes from praia, santiago island, cabo verde. | arthropod-borne viruses, such as dengue (denv), chikungunya (chikv), and zika (zikv), pose a challenge to public health, due to their worldwide distribution and large-scale outbreaks. dengue fever is currently one of the most important diseases and it is caused by four serotypes of denv and is mainly transmitted by the mosquito aedes aegypti. it is estimated that 50-100 million cases are reported every year worldwide. more recently, chikv and zikv, which are also transmitted by ae. aegypti, have ... | 2017 | 28973490 |
| chikungunya virus dissemination from the midgut of aedes aegypti is associated with temporal basal lamina degradation during bloodmeal digestion. | in the mosquito, the midgut epithelium is the initial tissue to become infected with an arthropod-borne virus (arbovirus) that has been acquired from a vertebrate host along with a viremic bloodmeal. following its replication in midgut epithelial cells, the virus needs to exit the midgut and infect secondary tissues including the salivary glands before it can be transmitted to another vertebrate host. the viral exit mechanism from the midgut, the midgut escape barrier (meb), is poorly understood ... | 2017 | 28961239 |
| use of rhodamine b to mark the body and seminal fluid of male aedes aegypti for mark-release-recapture experiments and estimating efficacy of sterile male releases. | recent interest in male-based sterile insect technique (sit) and incompatible insect technique (iit) to control aedes aegypti and aedes albopictus populations has revealed the need for an economical, rapid diagnostic tool for determining dispersion and mating success of sterilized males in the wild. previous reports from other insects indicated rhodamine b, a thiol-reactive fluorescent dye, administered via sugar-feeding can be used to stain the body tissue and seminal fluid of insects. here, we ... | 2017 | 28957318 |
| the distribution of aedes aegypti (diptera, culicidae) in eight selected parks of lahore, using oviposition traps during rainy season. | to investigate the seasonal distribution of immature habitat of aedes aegypti mosquito species and its association with environmental and climatic conditions. | 2017 | 28955062 |
| oviposition preferences of dengue vectors; aedes aegypti and aedes albopictus in sri lanka under laboratory settings. | investigations on oviposition behaviour of dengue vectors are critical for effective controlling of vector breeding. therefore, the aim of the present study was to determine the oviposition behaviour of dengue vectors, aedes aegypti and aedes albopictus in sri lanka. batches of 1000 adult mosquitoes (1 : 1, male: female ratio) housed in rearing cages were used for each experimental setup from ae. aegypti and ae. albopictus. oviposition responses with respect to the size of the ovitrap, colours o ... | 2017 | 28950922 |
| extensive genetic differentiation between homomorphic sex chromosomes in the mosquito vector, aedes aegypti. | mechanisms and evolutionary dynamics of sex-determination systems are of particular interest in insect vectors of human pathogens like mosquitoes because novel control strategies aim to convert pathogen-transmitting females into nonbiting males, or rely on accurate sexing for the release of sterile males. in aedes aegypti, the main vector of dengue and zika viruses, sex determination is governed by a dominant male-determining locus, previously thought to reside within a small, nonrecombining, se ... | 2017 | 28945882 |
| growth inhibitory effect of phenolic extracts of ziziphus jujuba mill. in dengue vector aedes aegypti (l) in parent and f1 generation. | to evaluate the lethal and emergence inhibitory effect of alkaloid, phenolic and terpenoid extracts of ziziphus jujuba (rhamnaceae) against aedes aegypti (diptera: culicidae), and to explore the effect of the most effective fraction on developmental and biochemical parameters of the dengue vector. | 2017 | 28942827 |
| chemical composition of buddleja polystachya aerial parts and its bioactivity against aedes aegypti. | a new acylatediridoid glycoside, 6-o-α-l-(2″-acetyl-4″-o-trans-isoferuloyl) rhamnopyranosyl catalpol (9) together with 18 known compounds belonging to the iridoids, flavonoids, triterpene saponin glycosides and phenylethanoids (1-8, 10-18) were isolated from the aerial parts and the flowers of buddleja polystachya. their structures were elucidated on the basis of spectroscopic evidence and comparison with that reported in the literature. promising adulticidal activity was shown for all extracts ... | 2017 | 28942684 |
| insights into the epidemiological link between biting flies and pemphigus foliaceus in southeastern brazil. | black fly and sandfly bites are related to the endemicity of pemphigus foliaceus (pf); however, an immune reaction against the salivary proteins from these flies still requires confirmation in the case of pf patients living in southeastern brazil. | 2017 | 28941730 |
| contribution of lysinibacillus sphaericus hemolysin and chitin-binding protein in entomopathogenic activity against insecticide resistant aedes aegypti. | given its toxicity against culicid larvae, lysinibacillus sphaericus is used for the biological control of mosquitoes such as culex sp. and anopheles sp. the toxicity factors currently reported for l. sphaericus include the binary toxin, mtx toxins, and the s-layer. however, aedes aegypti is refractory to the binary toxin, the most toxic larvicidal protein of l. sphaericus. until now, there are no evidences of the hemolytic and chitinolytic capacity of l. sphaericus. herein, the expression of th ... | 2017 | 28940156 |
| molecular typing and phylogeny of wolbachia: a study from assam, north-eastern part of india. | wolbachia are maternally inherited endosymbiotic alphaproteobacteria, infecting 40-75% of arthropod species. knowledge on distribution of native strains infecting mosquito vectors from endemic regions is essential for successful implementation of vector control interventions utilizing potential strains of wolbachia. study identified various native strains of wolbachia inhabiting different mosquito species from field and colonised conditions of assam. the fly drosophila melanogaster was also incl ... | 2017 | 28939497 |
| the dengue preface to endemic in mainland china: the historical largest outbreak by aedes albopictus in guangzhou, 2014. | dengue was regarded as a mild epidemic in mainland china transmitted by aedes albopictus. however, the 2014 record-breaking outbreak in guangzhou could change the situation. in order to provide an early warning of epidemic trends and provide evidence for prevention and control strategies, we seek to characterize the 2014 outbreak through application of detailed cases and entomological data, as well as phylogenetic analysis of viral envelope (e) gene. | 2017 | 28934991 |
| isolation and identification of xenorhabdus and photorhabdus bacteria associated with entomopathogenic nematodes and their larvicidal activity against aedes aegypti. | aedes aegypti is a potential vector of west nile, japanese encephalitis, chikungunya, dengue and zika viruses. alternative control measurements of the vector are needed to overcome the problems of environmental contamination and chemical resistance. xenorhabdus and photorhabdus are symbionts in the intestine of entomopathogenic nematodes (epns) steinernema spp. and heterorhabditis spp. these bacteria are able to produce a broad range of bioactive compounds including antimicrobial, antiparasitic, ... | 2017 | 28934970 |
| proteomics technique opens new frontiers in mobilome research. | a large proportion of the genome of most eukaryotic organisms consists of highly repetitive mobile genetic elements. the sum of these elements is called the "mobilome," which in eukaryotes is made up mostly of transposons. transposable elements contribute to disease, evolution, and normal physiology by mediating genetic rearrangement, and through the "domestication" of transposon proteins for cellular functions. although 'omics studies of mobilome genomes and transcriptomes are common, technical ... | 2017 | 28932623 |
| zika virus congenital syndrome: experimental models and clinical aspects. | viral infections have long been the cause of severe diseases to humans, increasing morbidity and mortality rates worldwide, either in rich or poor countries. yellow fever virus, h1n1 virus, hiv, dengue virus, hepatitis b and c are well known threats to human health, being responsible for many million deaths annually, associated to a huge economic and social cost. in this context, a recently introduced flavivirus in south america, called zika virus (zikv), led the who to declare in february 1st 2 ... | 2017 | 28932235 |
| ph control in the midgut of aedesaegypti under different nutritional conditions. | aedes aegypti is one of the most important disease vectors in the world. because their gut is the first site of interaction with pathogens, it is important to understand a. aegypti gut physiology. in this study, we investigated the mechanisms of ph control in the midgut of a. aegypti females under different nutritional conditions. we found that unfed females have an acidic midgut (ph ∼6). the midgut of unfed insects is actively maintained at ph 6 regardless of the ingestion of either alkaline or ... | 2017 | 28931720 |
| microgeographic population structuring of aedes aegypti (diptera: culicidae). | aedes aegypti is one of the species most favored by changes in the environment caused by urbanization. its abundance increases rapidly in the face of such changes, increasing the risk of disease transmission. previous studies have shown that mosquito species that have adapted to anthropogenic environmental changes benefit from urbanization and undergo population expansion. in light of this, we used microsatellite markers to explore how urbanization processes may be modulating ae. aegypti populat ... | 2017 | 28931078 |
| pyrethroid resistance alters the blood-feeding behavior in puerto rican aedes aegypti mosquitoes exposed to treated fabric. | emerging insecticide resistance is a major issue for vector control. it decreases the effectiveness of insecticides, thereby requiring greater quantities for comparable control with a net increase in risk of disease resurgence, product cost, and damage risk to the ecosystem. pyrethroid resistance has been documented in puerto rican populations of aedes aegypti (l.) mosquitoes. in this study, topical toxicity of five insecticides (permethrin, etofenprox, deltamethrin, ddt, transfluthrin) was dete ... | 2017 | 28931018 |
| emerging arboviruses in rio grande do sul, brazil: chikungunya and zika outbreaks, 2014-2016. | the recent emergence of arboviruses such as chikungunya virus (chikv) and zika virus (zikv) in brazil has posed a threat to human health and to the country's economy. outbreaks occur mainly in tropical areas; however, increasing number of cases have been observed in rio grande do sul (rs), the southernmost state; therefore, surveillance of these arboviruses is essential for public health measures. | 2017 | 28929534 |
| sybr green-based one step quantitative real-time polymerase chain reaction assay for the detection of zika virus in field-caught mosquitoes. | the monitoring of vectors is one of the key surveillance measures to assess the risk of arbovirus transmission and the success of control strategies in endemic regions. the recent re-emergence of zika virus (zikv) in the tropics, including singapore, emphasizes the need to develop cost-effective, rapid and accurate assays to monitor the virus spread by mosquitoes. as zikv infections largely remain asymptomatic, early detection of zikv in the field-caught mosquitoes enables timely implementation ... | 2017 | 28927458 |
| insecticide susceptibility of aedes albopictus and ae. aegypti from brazil and the swiss-italian border region. | aedes aegypti and ae. albopictus are two highly invasive mosquito species, both vectors of several viruses, including dengue, chikungunya and zika. while ae. aegypti is the primary vector in the tropics and sub-tropics, ae. albopictus is increasingly under the public health watch as it has been implicated in arbovirus-transmission in more temperate regions, including continental europe. vector control using insecticides is the pillar of most control programmes; hence development of insecticide r ... | 2017 | 28927441 |
| community participation in mosquito breeding site control: an interdisciplinary mixed methods study in curaçao. | as the arboviral diseases dengue, chikungunya and zika emerge in the americas, so does the need for sustainable vector control policies. to successfully achieve mosquito control, joint efforts of both communities and governments are essential. this study investigates this important, but by-and-large neglected topic. | 2017 | 28927437 |
| differential expression of putative sodium-dependent cation-chloride cotransporters in aedes aegypti. | the yellow fever mosquito, aedes aegypti, has three genes that code for proteins with sequence similarity to vertebrate na+-k+-cl- cotransporters (nkccs) of the solute-linked carrier 12 superfamily of cation-chloride cotransporters (cccs). we hypothesized that these mosquito nkcc orthologues have diverged to perform distinct roles in salt secretion and absorption. in phylogenetic analyses, one protein (aenkcc1) groups with a drosophila melanogaster nkcc that mediates salt secretion whereas two o ... | 2017 | 28923771 |
| role of the venus kinase receptor in the female reproductive physiology of the desert locust, schistocerca gregaria. | venus kinase receptors (vkr) are a subfamily of invertebrate receptor tyrosine kinases, which have only recently been discovered. they contain an intracellular tyrosine kinase domain and an extracellular venus flytrap domain. vkrs have been functionally and pharmacologically characterized in only two invertebrate species, namely the human parasite schistosoma mansoni and the mosquito aedes aegypti, where they play a crucial role in oogenesis. here, we report the characterization of a vkr in the ... | 2017 | 28916758 |
| evaluation of ecdysteroid antisera for a competitive enzyme immunoassay and extraction procedures for the measurement of mosquito ecdysteroids. | ecdysteroid hormones regulate several aspects of insect development and reproduction. the predominant ecdysteroids produced by insects including mosquitoes are ecdysone (e) and 20-hydroxyecdysone (20e). the ability to measure e and 20e titers is essential for many studies, but few sensitive, low cost options are currently available for doing so. to address this deficiency, we developed a new enzyme-linked immunoassay (eia). in the first part of the study, we compared the affinity of two new anti ... | 2017 | 28866256 |
| aedes aegypti saliva enhances chikungunya virus replication in human skin fibroblasts via inhibition of the type i interferon signaling pathway. | chikungunya virus (chikv) transmission occurs through the bite of an infected aedes mosquito which injects virus-containing saliva into the skin of the human host during blood feeding. in the present study, we have determined the effect of aedes aegypti saliva on chikv replication in human skin fibroblasts, a major cell type for viral entry, which mimics the events that occur during natural transmission. a significant increase in the expression of viral transcripts and infectious viral particles ... | 2017 | 28866137 |
| presence of a predator image in potential breeding sites and oviposition responses of a dengue vector. | in dengue vector control, attempts to minimize or replace the use of pesticides have mostly involved use of predators, but success has been severely impeded by difficulties associated with financial and environmental costs, predator mass production, and persistence in target habitats. visual deterrents have been used successfully to control animal pests, in some cases in an effort to replace pesticide use. despite evidence that visual signals are crucial in site choice for egg deposition by deng ... | 2017 | 28865898 |