Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| comparative analysis of dengue and zika outbreaks reveals differences by setting and virus. | the pacific islands of micronesia have experienced several outbreaks of mosquito-borne diseases over the past decade. in outbreaks on small islands, the susceptible population is usually well defined, and there is no co-circulation of pathogens. because of this, analysing such outbreaks can be useful for understanding the transmission dynamics of the pathogens involved, and particularly so for yet understudied pathogens such as zika virus. here, we compared three outbreaks of dengue and zika vir ... | 2016 | 27926933 |
| aedes (stegomyia) albopictus' dynamics influenced by spatiotemporal characteristics in a brazilian dengue-endemic risk city. | brazil reported the majority of the dengue cases in americas during the last two decades, where the occurrence of human dengue cases is exclusively attributed to the aedes (stegomyia) aegypti (linnaeus). nowadays, other recognized dengue virus (denv) vector in asian countries, aedes (stegomyia) albopictus (skuse), has been detected in more than half of the 5565 brazilian municipalities. therefore, the aim of the present study was to investigate the presence of, and determine the ae. albopictus' ... | 2016 | 27771419 |
| analysis of synonymous codon usage bias of zika virus and its adaption to the hosts. | zika virus (zikv) is a mosquito-borne virus (arbovirus) in the family flaviviridae, and the symptoms caused by zikv infection in humans include rash, fever, arthralgia, myalgia, asthenia and conjunctivitis. codon usage bias analysis can reveal much about the molecular evolution and host adaption of zikv. to gain insight into the evolutionary characteristics of zikv, we performed a comprehensive analysis on the codon usage pattern in 46 zikv strains by calculating the effective number of codons ( ... | 2016 | 27893824 |
| a prospective cohort study to assess seroprevalence, incidence, knowledge, attitudes and practices, willingness to pay for vaccine and related risk factors in dengue in a high incidence setting. | dengue is one of the most important vector-borne diseases in the world, causing significant morbidity and economic impact. in colombia, dengue is a major public health problem. departments of la guajira, cesar and magdalena are dengue endemic areas. the objective of this research is to determine the seroprevalence and the incidence of dengue virus infection in the participating municipalities from these departments, and also establish the association between individual and housing factors and ve ... | 2016 | 27887591 |
| tsetse fly tolerance to t. brucei infection: transcriptome analysis of trypanosome-associated changes in the tsetse fly salivary gland. | for their transmission, african trypanosomes rely on their blood feeding insect vector, the tsetse fly (glossina sp.). the ingested trypanosoma brucei parasites have to overcome a series of barriers in the tsetse fly alimentary tract to finally develop into the infective metacyclic forms in the salivary glands that are transmitted to a mammalian host by the tsetse bite. the parasite population in the salivary gland is dense with a significant number of trypanosomes tightly attached to the epithe ... | 2016 | 27884110 |
| susceptibility profile and metabolic mechanisms involved in aedes aegypti and aedes albopictus resistant to ddt and deltamethrin in the central african republic. | aedes aegypti and ae. albopictus are the main epidemic vectors of dengue, chikungunya and zika viruses worldwide. their control during epidemics relies mainly on control of larvae and adults with insecticides. unfortunately, loss of susceptibility of both species to several insecticide classes limits the efficacy of interventions. in africa, where aedes-borne viruses are of growing concern, few data are available on resistance to insecticides. to fill this gap, we assessed the susceptibility to ... | 2016 | 27881148 |
| generation of monoclonal antibodies against native viral proteins using antigen-expressing mammalian cells for mouse immunization. | due to their rising incidence and progressive geographical spread, infections with mosquito-borne viruses, such as dengue (denv), chikungunya and zika virus, have developed into major public health challenges. since all of these viruses may cause similar symptoms and can occur in concurrent epidemics, tools for their differential diagnosis and epidemiological monitoring are of urgent need. | 2016 | 27876044 |
| the driving force for 2014 dengue outbreak in guangdong, china. | dengue fever has rapidly spread in recent decades to become the most globally expansive viral vector-borne disease. in mainland china, a number of dengue outbreaks have been reported since 1978, but the worst epidemic in decades, involving 45230 cases and 76 imported cases, resulting in six deaths in guangdong province, emerged in 2014. reasons for this ongoing surge in dengue, both imported and autochthonous, are currently unclear and demand urgent investigation. here, a seasonally-driven dynam ... | 2016 | 27861514 |
| genomic studies of envelope gene sequences from mosquito and human samples from bangkok, thailand. | dengue virus (denv) is an rna virus showing a high degree of genetic variation as a consequence of its proofreading inability. this variation plays an important role in virus evolution and pathogenesis. although levels of within-host genetic variation are similar following equilibrium, variation among different hosts is frequently different. to identify dengue quasispecies present among two hosts, we collected patient samples from six acute denv cases and two pools of aedes aegypti mosquitoes an ... | 2016 | 27917342 |
| detection of dengue virus serotypes 1, 2 and 3 in selected regions of kenya: 2011-2014. | dengue fever, a mosquito-borne disease, is associated with illness of varying severity in countries in the tropics and sub tropics. dengue cases continue to be detected more frequently and its geographic range continues to expand. we report the largest documented laboratory confirmed circulation of dengue virus in parts of kenya since 1982. | 2016 | 27814732 |
| comprehensive dna methylation analysis of the aedes aegypti genome. | aedes aegypti mosquitoes are important vectors of viral diseases. mosquito host factors play key roles in virus control and it has been suggested that dengue virus replication is regulated by dnmt2-mediated dna methylation. however, recent studies have shown that dnmt2 is a trna methyltransferase and that dnmt2-dependent methylomes lack defined dna methylation patterns, thus necessitating a systematic re-evaluation of the mosquito genome methylation status. we have now searched the ae. aegypti g ... | 2016 | 27805064 |
| dengue outbreak in mombasa city, kenya, 2013-2014: entomologic investigations. | dengue outbreaks were first reported in east africa in the late 1970s to early 1980s including the 1982 outbreak on the kenyan coast. in 2011, dengue outbreaks occurred in mandera in northern kenya and subsequently in mombasa city along the kenyan coast in 2013-2014. following laboratory confirmation of dengue fever cases, an entomologic investigation was conducted to establish the mosquito species, and densities, causing the outbreak. affected parts of the city were identified with the help of ... | 2016 | 27783626 |
| the interplay of vaccination and vector control on small dengue networks. | dengue fever is a major public health issue affecting billions of people in over 100 countries across the globe. this challenge is growing as the invasive mosquito vectors, aedes aegypti and aedes albopictus, expand their distributions and increase their population sizes. hence there is an increasing need to devise effective control methods that can contain dengue outbreaks. here we construct an epidemiological model for virus transmission between vectors and hosts on a network of host populatio ... | 2016 | 27457093 |
| zika mosquito vectors: the jury is still out. | after a 40-year hiatus, the international congress of entomology (ice 2016) convened in orlando, florida (september 25-30, 2016). one of the symposia at ice 2016, the zika symposium, covered multiple aspects of the zika epidemic, including epidemiology, sexual transmission, genetic tools for reducing transmission, and particularly vector competence. while there was a consensus among participants that the yellow fever mosquito, aedes aegypti, is a vector of the zika virus, there is growing eviden ... | 2016 | 27853521 |
| polyphenol-rich diets exacerbate ampk-mediated autophagy, decreasing proliferation of mosquito midgut microbiota, and extending vector lifespan. | mosquitoes feed on plant-derived fluids such as nectar and sap and are exposed to bioactive molecules found in this dietary source. however, the role of such molecules on mosquito vectorial capacity is unknown. weather has been recognized as a major determinant of the spread of dengue, and plants under abiotic stress increase their production of polyphenols. | 2016 | 27732590 |
| emergence of epidemic dengue-1 virus in the southern province of sri lanka. | dengue is a frequent cause of acute febrile illness with an expanding global distribution. since the 1960s, dengue in sri lanka has been documented primarily along the heavily urbanized western coast with periodic shifting of serotypes. outbreaks from 2005-2008 were attributed to a new clade of denv-3 and more recently to a newly introduced genotype of denv-1. in 2007, we conducted etiologic surveillance of acute febrile illness in the southern province and confirmed dengue in only 6.3% of febri ... | 2016 | 27711206 |
| epidemiological and molecular characterization of dengue viruses imported into guangzhou during 2009-2013. | dengue virus causes one of the most significant infectious diseases in tropical and subtropical regions, notable number of which is imported into china every year. | 2016 | 27722053 |
| epidemiologic investigation of a family cluster of imported zikv cases in guangdong, china: probable human-to-human transmission. | zika virus (zikv) is an emerging mosquito-borne flavivirus that can potentially threaten south china. a chinese family of four returning from venezuela to china was found to be positive for zikv when the youngest son's fever was first detected at an airport immigration inspection. they were isolated temporarily in a local hospital in enping city, guangdong province, where their clinical data were recorded and urine and saliva were collected to isolate zikv and to obtain viral sequences. all of t ... | 2016 | 27599469 |
| the importance of socio-economic versus environmental risk factors for reported dengue cases in java, indonesia. | dengue is a major mosquito-borne viral disease and an important public health problem. identifying which factors are important determinants in the risk of dengue infection is critical in supporting and guiding preventive measures. in south-east asia, half of all reported fatal infections are recorded in indonesia, yet little is known about the epidemiology of dengue in this country. | 2016 | 27603137 |
| maintenance of demographic and hematological profiles in a long-lasting dengue fever outbreak: implications for management. | dengue fever (df) outbreaks present regionally specific epidemiological and clinical characteristics. in certain medium-sized cities (100 000-250 000 inhabitants) of são paulo state, brazil, and after reaching an incidence of 150 cases/100 000 inhabitants ("epidemiological threshold"), clinical diagnosis indicated dengue virus (denv) infection. during this period, other seasonally infectious diseases with symptoms and physical signs mimicking df can simultaneously occur, with the consequential o ... | 2016 | 27593529 |
| measuring haitian children's exposure to chikungunya, dengue and malaria. | to differentiate exposure to the newly introduced chikungunya virus from exposure to endemic dengue virus and other pathogens in haiti. | 2016 | 27821884 |
| patterned array of poly(ethylene glycol) silane monolayer for label-free detection of dengue. | in the present study, the construction of arrays on silicon for naked-eye detection of dna dengue was demonstrated. the array was created by exposing a polyethylene glycol (peg) silane monolayer to 254 nm ultraviolet (uv) light through a photomask. formation of the peg silane monolayer and photomodifed surface properties was thoroughly characterized by using atomic force microscopy (afm), x-ray photoelectron spectroscopy (xps), and contact angle measurements. the results of xps confirmed that ir ... | 2016 | 27571080 |
| impact of dengue virus (denv) co-infection on clinical manifestations, disease severity and laboratory parameters. | the co-circulation of 4 denv serotypes in geographically expanding area, has resulted in increasing occurrence of denv co-infections. however, studies assessing the clinical impact of denv co-infections have been scarce and have involved small number of patients. this study explores the impact of denv co-infection on clinical manifestations and laboratory parameters. | 2016 | 27514512 |
| extended evaluation of virological, immunological and pharmacokinetic endpoints of celaden: a randomized, placebo-controlled trial of celgosivir in dengue fever patients. | celaden was a randomized placebo-controlled trial of 50 patients with confirmed dengue fever to evaluate the efficacy and safety of celgosivir (a study registered at clinicaltrials.gov, number nct01619969). celgosivir was given as a 400 mg loading dose and 200 mg bid (twice a day) over 5 days. replication competent virus was measured by plaque assay and compared to reverse transcription quantitative pcr (qpcr) of viral rna. pharmacokinetics (pk) correlations with viremia, immunological profiling ... | 2016 | 27509020 |
| prospects for dengue vaccines for travelers. | travel-acquired dengue cases have been increasing as the overall global dengue burden has expanded. in korea, imported dengue cases have been reported since 2000 when it first became a notifiable disease. during the first four months of 2016, three times more dengue cases were reported in korea than during the same period the previous year. a safe and efficacious vaccine for travelers would be beneficial to prevent dengue disease in individual travelers and potentially decrease the risk of virus ... | 2016 | 27489798 |
| storm drains as larval development and adult resting sites for aedes aegypti and aedes albopictus in salvador, brazil. | dengue (denv), chikungunya (chikv), zika (zikv), as well as yellow fever (yfv) viruses are transmitted to humans by aedes spp. females. in salvador, the largest urban center in north-eastern brazil, the four denv types have been circulating, and more recently, chikv and zikv have also become common. we studied the role of storm drains as aedes larval development and adult resting sites in four neighbourhoods of salvador, representing different socioeconomic, infrastructure and topographic condit ... | 2016 | 27464886 |
| effect of spatial repellent exposure on dengue vector attraction to oviposition sites. | aedes aegypti is a primary vector of dengue virus (denv), the causative agent of dengue fever, an arthropod-borne disease of global importance. although a vaccine has been recommended for prevention, current dengue prevention strategies rely on vector control. recently, volatile pyrethroids-spatial repellents-have received interest as a novel delivery system for adult ae. aegypti control. understanding the full range of behavioral effects spatial repellents elicit in mosquito species will be cri ... | 2016 | 27428011 |
| zika virus challenges for neuropsychiatry. | before 2007, zika virus (zikv) was generally considered as an arbovirus of low clinical relevance, causing a mild self-limiting febrile illness in tropical africa and southeast asia. currently, a large, ongoing outbreak of zikv that started in brazil in 2015 is spreading across the americas. virus infection during pregnancy has been potentially linked to congenital malformations, including microcephaly. in addition to congenital malformations, a temporal association between zikv infection and an ... | 2016 | 27478378 |
| applications of a rapid and sensitive dengue duo rapid immunochromatographic test kit as a diagnostic strategy during a dengue type 2 epidemic in an urban city. | dengue infection is a major health problem in tropical and subtropical countries. a prospective observational study in a university-affiliated hospital was conducted between august 2015 and september 2015. patients who visited the emergency department (ed) with a presentation of any symptoms of dengue were eligible for the dengue non-structural protein 1 (ns1), igm/igg rapid immunochromatographic tests and real-time polymerase chain reaction (rt-pcr) to evaluate the performance of the rapid test ... | 2016 | 27415767 |
| serotype influences on dengue severity: a cross-sectional study on 485 confirmed dengue cases in vitória, brazil. | dengue is caused by a rna virus of the family flaviviridae, which presents four serotypes (denv-1 to denv-4) capable of inducing hemorrhage. the purpose of this study was to evaluate the influence of serotype on the outcome of dengue. | 2016 | 27393011 |
| dengue virus sero-cross-reactivity drives antibody-dependent enhancement of infection with zika virus. | zika virus (zikv) was discovered in 1947 and was thought to lead to relatively mild disease. the recent explosive outbreak of zikv in south america has led to widespread concern, with reports of neurological sequelae ranging from guillain barré syndrome to microcephaly. zikv infection has occurred in areas previously exposed to dengue virus (denv), a flavivirus closely related to zikv. here we investigated the serological cross-reaction between the two viruses. plasma immune to denv showed subst ... | 2016 | 27339099 |
| epidemic resurgence of dengue fever in singapore in 2013-2014: a virological and entomological perspective. | dengue resurged in singapore during 2013-14, causing an outbreak with unprecedented number of cases in the country. in the present study, we summarise the epidemiological, virological and entomological findings gathered through the dengue surveillance programme and highlight the drivers of the epidemic. we also describe how the surveillance system facilitated the preparedness to moderate epidemic transmission of dengue in the country. | 2016 | 27316694 |
| virus-like particles derived from pichia pastoris-expressed dengue virus type 1 glycoprotein elicit homotypic virus-neutralizing envelope domain iii-directed antibodies. | four antigenically distinct serotypes (1-4) of dengue viruses (denvs) cause dengue disease. antibodies to any one denv serotype have the potential to predispose an individual to more severe disease upon infection with a different denv serotype. a dengue vaccine must elicit homotypic neutralizing antibodies to all four denv serotypes to avoid the risk of such antibody-dependent enhancement in the vaccine recipient. this is a formidable challenge as evident from the lack of protective efficacy aga ... | 2016 | 27301568 |
| assessing seasonal risks for the introduction and mosquito-borne spread of zika virus in europe. | the explosive zika virus epidemic in the americas is amplifying spread of this emerging pathogen into previously unaffected regions of the world, including europe (gulland, 2016), where local populations are immunologically naïve. as summertime approaches in the northern hemisphere, aedes mosquitoes in europe may find suitable climatic conditions to acquire and subsequently transmit zika virus from viremic travellers to local populations. while aedes albopictus has proven to be a vector for the ... | 2016 | 27344225 |
| population genetics of the asian tiger mosquito aedes albopictus, an invasive vector of human diseases. | the asian tiger mosquito aedes albopictus is currently one of the most threatening invasive species in the world. native to southeast asia, the species has spread throughout the world in the past 30 years and is now present in every continent but antarctica. because it was the main vector of recent dengue and chikungunya outbreaks, and because of its competency for numerous other viruses and pathogens such as the zika virus, a. albopictus stands out as a model species for invasive diseases vecto ... | 2016 | 27273325 |
| particularities of allergy in the tropics. | allergic diseases are distributed worldwide and their risk factors and triggers vary according to geographical and socioeconomic conditions. allergies are frequent in the tropics but aspects of their prevalence, natural history, risk factors, sensitizers and triggers are not well defined and some are expected to be different from those in temperate zone countries. the aim of this review is to investigate if allergic diseases in the tropics have particularities that deserve special attention for ... | 2016 | 27386040 |
| antinociceptive effect of tephrosia sinapou extract in the acetic acid, phenyl-p-benzoquinone, formalin, and complete freund's adjuvant models of overt pain-like behavior in mice. | tephrosia toxicaria, which is currently known as tephrosia sinapou (buc'hoz) a. chev. (fabaceae), is a source of compounds such as flavonoids. t. sinapou has been used in amazonian countries traditional medicine to alleviate pain and inflammation. the purpose of this study was to evaluate the analgesic effects of t. sinapou ethyl acetate extract in overt pain-like behavior models in mice by using writhing response and flinching/licking tests. we demonstrated in this study that t. sinapou extract ... | 2016 | 27293981 |
| a deep insight into the sialome of male and female aedes aegypti mosquitoes. | only adult female mosquitoes feed on blood, while both genders take sugar meals. accordingly, several compounds associated with blood feeding (i.e. vasodilators, anti-clotting, anti-platelets) are found only in female glands, while enzymes associated with sugar feeding or antimicrobials (such as lysozyme) are found in the glands of both sexes. we performed de novo assembly of reads from adult aedes aegypti female and male salivary gland libraries (285 and 90 million reads, respectively). by mapp ... | 2016 | 26999592 |
| epidemiology of chikungunya virus in bahia, brazil, 2014-2015. | chikungunya is an emerging arbovirus that is characterized into four lineages. one of these, the asian genotype, has spread rapidly in the americas after its introduction in the saint martin island in october 2013. unexpectedly, a new lineage, the east-central-south african genotype, was introduced from angola in the end of may 2014 in feira de santana (fsa), the second largest city in bahia state, brazil, where over 5,500 cases have now been reported. number weekly cases of clinically confirmed ... | 2016 | 27330849 |
| accuracy of clinical criteria and an immunochromatographic strip test for dengue diagnosis in a denv-4 epidemic. | early diagnosis of dengue infection is important for decision-making and timely implementation of therapeutic measures. although rapid ns1 assays have been used for dengue diagnosis since 2008, their performance in denv-4 cases has not yet been fully assessed. | 2016 | 26822788 |
| in silico analysis of an envelope domain iii-based multivalent fusion protein as a potential dengue vaccine candidate. | dengue virus infection is now a global problem. currently, there is no licensed vaccine or proven antiviral treatment against this virus. all four serotypes (1-4) of dengue virus can infect human. an effective dengue vaccine should be tetravalent to induce protective immune responses against all four serotypes. most of dengue vaccine candidates are monovalent, or in the form of physically mixed multivalent formulations. recently envelope protein domain iii of virus is considered as a vaccine can ... | 2016 | 26866023 |
| the risk of dengue virus transmission in dar es salaam, tanzania during an epidemic period of 2014. | in 2010, 2012, 2013 and 2014 dengue outbreaks have been reported in dar es salaam, tanzania. however, there is no comprehensive data on the risk of transmission of dengue in the country. the objective of this study was to assess the risk of transmission of dengue in dar es salaam during the 2014 epidemic. | 2016 | 26812489 |
| identification of proteins bound to dengue viral rna in vivo reveals new host proteins important for virus replication. | dengue virus is the most prevalent cause of arthropod-borne infection worldwide. due to the limited coding capacity of the viral genome and the complexity of the viral life cycle, host cell proteins play essential roles throughout the course of viral infection. host rna-binding proteins mediate various aspects of virus replication through their physical interactions with viral rna. here we describe a technique designed to identify such interactions in the context of infected cells using uv cross ... | 2016 | 26733069 |
| relevance of non-communicable comorbidities for the development of the severe forms of dengue: a systematic literature review. | patients with dengue fever and comorbidities seem to be at higher risk of developing complications and/or severe dengue compared to healthier individuals. this study systematically reviews the evidence related to comorbidities and dengue. a systematic literature review was performed in five databases (embase, pubmed, global health, scielo, cochrane) and grey literature for full-text articles since its inceptions until october 10, 2015. a total of 230 articles were retrieved. sixteen studies were ... | 2016 | 26727113 |
| antibody-dependent enhancement of dengue virus infection inhibits rlr-mediated type-i ifn-independent signalling through upregulation of cellular autophagy. | antibody dependent enhancement (ade) of dengue virus (denv) infection is identified as the main risk factor of severe dengue diseases. through opsonization by subneutralizing or non-neutralizing antibodies, denv infection suppresses innate cell immunity to facilitate viral replication. however, it is largely unknown whether suppression of type-i ifn is necessary for a successful ade infection. here, we report that both denv and denv-ade infection induce an early isg (nos2) expression through rlr ... | 2016 | 26923481 |
| in silico screening, alanine mutation, and dft approaches for identification of ns2b/ns3 protease inhibitors. | to identify the ligand that binds to a target protein with high affinity is a nontrivial task in computer-assisted approaches. antiviral drugs have been identified for ns2b/ns3 protease enzyme on the mechanism to cleave the viral protein using the computational tools. the consequence of the molecular docking, free energy calculations, and simulation protocols explores the better ligand. it provides in-depth structural insights with the catalytic triad of his51, asp75, ser135, and gly133. the md ... | 2016 | 27057355 |
| no evidence for local adaptation of dengue viruses to mosquito vector populations in thailand. | despite their epidemiological importance, the evolutionary forces that shape the spatial structure of dengue virus genetic diversity are not fully understood. fine-scale genetic structure of mosquito vector populations and evidence for genotype × genotype interactions between dengue viruses and their mosquito vectors are consistent with the hypothesis that the geographical distribution of dengue virus genetic diversity may reflect viral adaptation to local mosquito populations. to test this hypo ... | 2016 | 27099625 |
| phylogenetic analysis of dengue virus 1 isolated from south minas gerais, brazil. | dengue is a major worldwide public health problem, especially in the tropical and subtropical regions of the world. primary infection with a single dengue virus serotype causes a mild, self-limiting febrile illness called dengue fever. however, a subset of patients who experience secondary infection with a different serotype can progress to a more severe form of the disease, called dengue hemorrhagic fever. the four dengue virus serotypes (1-4) are antigenically and genetically distinct and each ... | 2016 | 26887252 |
| the risk of dengue for non-immune foreign visitors to the 2016 summer olympic games in rio de janeiro, brazil. | rio de janeiro in brazil will host the summer olympic games in 2016. about 400,000 non-immune foreign tourists are expected to attend the games. as brazil is the country with the highest number of dengue cases worldwide, concern about the risk of dengue for travelers is justified. | 2016 | 27129407 |
| analysis of significant factors for dengue fever incidence prediction. | many popular dengue forecasting techniques have been used by several researchers to extrapolate dengue incidence rates, including the k-h model, support vector machines (svm), and artificial neural networks (ann). the time series analysis methodology, particularly arima and sarima, has been increasingly applied to the field of epidemiological research for dengue fever, dengue hemorrhagic fever, and other infectious diseases. the main drawback of these methods is that they do not consider other v ... | 2016 | 27083696 |
| first chikungunya outbreak in suriname; clinical and epidemiological features. | in june 2014, suriname faced the first chikungunya outbreak. since international reports mostly focus on hospitalized patients, the least affected group, a study was conducted to describe clinical characteristics of mainly outpatients including children. in addition, the cumulative incidence of this first epidemic was investigated. | 2016 | 27082985 |
| assessing potential countermeasures against the dengue epidemic in non-tropical urban cities. | dengue is a common mosquito-borne viral disease epidemic especially in tropical and sub-tropical regions where water sanitation is not substantially controlled. however, dengue epidemics sometimes occur in non-tropical urban cities with substantial water sanitary control. using a mathematical model, we investigate what conditions can be important for a dengue epidemic to occur in an urban city such as tokyo, where vectors are active only in summer and there are little number of vectors around ho ... | 2016 | 27072122 |
| epidemiological dynamics of an urban dengue 4 outbreak in são paulo, brazil. | dengue studies at the urban scale are scarce and required for guiding control efforts. in brazil, the burden of dengue is high and challenges city public health administrations with limited resources. here we studied the dynamics of a dengue epidemic in a single city. | 2016 | 27069820 |
| presence of three dengue serotypes in ouagadougou (burkina faso): research and public health implications. | the significant malaria burden in africa has often eclipsed other febrile illnesses. burkina faso's first dengue epidemic occurred in 1925 and the most recent in 2013. yet there is still very little known about dengue prevalence, its vector proliferation, and its poverty and equity impacts. | 2016 | 27044528 |
| fitting the incidence data from the city of campinas, brazil, based on dengue transmission modellings considering time-dependent entomological parameters. | four time-dependent dengue transmission models are considered in order to fit the incidence data from the city of campinas, brazil, recorded from october 1st 1995 to september 30th 2012. the entomological parameters are allowed to depend on temperature and precipitation, while the carrying capacity and the hatching of eggs depend only on precipitation. the whole period of incidence of dengue is split into four periods, due to the fact that the model is formulated considering the circulation of o ... | 2016 | 27010654 |
| evolutionary analysis of dengue serotype 2 viruses using phylogenetic and bayesian methods from new delhi, india. | dengue fever is the most important arboviral disease in the tropical and sub-tropical countries of the world. delhi, the metropolitan capital state of india, has reported many dengue outbreaks, with the last outbreak occurring in 2013. we have recently reported predominance of dengue virus serotype 2 during 2011-2014 in delhi. in the present study, we report molecular characterization and evolutionary analysis of dengue serotype 2 viruses which were detected in 2011-2014 in delhi. envelope genes ... | 2016 | 26977703 |
| dengue in java, indonesia: relevance of mosquito indices as risk predictors. | no vaccine is currently available for dengue virus (denv), therefore control programmes usually focus on managing mosquito vector populations. entomological surveys provide the most common means of characterising vector populations and predicting the risk of local dengue virus transmission. despite indonesia being a country strongly affected by denv, only limited information is available on the local factors affecting denv transmission and the suitability of available survey methods for assessin ... | 2016 | 26967524 |
| co-distribution and co-infection of chikungunya and dengue viruses. | chikungunya and dengue infections are spatio-temporally related. the current review aims to determine the geographic limits of chikungunya, dengue and the principal mosquito vectors for both viruses and to synthesise current epidemiological understanding of their co-distribution. | 2016 | 26936191 |
| high-throughput cis-regulatory element discovery in the vector mosquito aedes aegypti. | despite substantial progress in mosquito genomic and genetic research, few cis-regulatory elements (cres), dna sequences that control gene expression, have been identified in mosquitoes or other non-model insects. formaldehyde-assisted isolation of regulatory elements paired with dna sequencing, faire-seq, is emerging as a powerful new high-throughput tool for global cre discovery. faire results in the preferential recovery of open chromatin dna fragments that are not bound by nucleosomes, an ev ... | 2016 | 27161480 |
| projected impact of dengue vaccination in yucatán, mexico. | dengue vaccines will soon provide a new tool for reducing dengue disease, but the effectiveness of widespread vaccination campaigns has not yet been determined. we developed an agent-based dengue model representing movement of and transmission dynamics among people and mosquitoes in yucatán, mexico, and simulated various vaccine scenarios to evaluate effectiveness under those conditions. this model includes detailed spatial representation of the yucatán population, including the location and mov ... | 2016 | 27227883 |
| temporal dynamics and spatial patterns of aedes aegypti breeding sites, in the context of a dengue control program in tartagal (salta province, argentina). | since 2009, fundación mundo sano has implemented an aedes aegypti surveillance and control program in tartagal city (salta province, argentina). the purpose of this study was to analyze temporal dynamics of ae. aegypti breeding sites spatial distribution, during five years of samplings, and the effect of control actions over vector population dynamics. | 2016 | 27223693 |
| meta-analysis of dengue severity during infection by different dengue virus serotypes in primary and secondary infections. | dengue virus (denv) infection is currently a major cause of morbidity and mortality in the world; it has become more common and virulent over the past half-century and has gained much attention. thus, this review compared the percentage of severe cases of both primary and secondary infections with different serotypes of dengue virus. | 2016 | 27213782 |
| proceedings of the 3rd ipleiria's international health congress : leiria, portugal. 6-7 may 2016. | 2016 | 27409075 | |
| a review of chikungunya virus-induced arthralgia: clinical manifestations, therapeutics, and pathogenesis. | chikungunya virus (chikv) is a mosquito-borne alphavirus that circulates predominantly in tropical and subtropical regions, potentially affecting over 1 billion people. recently, an outbreak began in the western hemisphere and has resulted in over 1.8 million reported suspected cases. infection often results in severe fever, rash and debilitating polyarthralgia lasting weeks to months. additionally, the current literature reports that chikv can result in a severe chronic arthralgia and/or arthri ... | 2016 | 28077980 |
| analysis of chikungunya virus proteins reveals that non-structural proteins nsp2 and nsp3 exhibit rna interference (rnai) suppressor activity. | rnai pathway is an antiviral defence mechanism employed by insects that result in degradation of viral rna thereby curbing infection. several viruses including flaviviruses encode viral suppressors of rnai (vsrs) to counteract the antiviral rnai pathway. till date, no vsr has been reported in alphaviruses. the present study was undertaken to evaluate chikungunya virus (chikv) proteins for rnai suppressor activity. we systematically analyzed all nine chikv proteins for rnai suppressor activity us ... | 2016 | 27901124 |
| diet-induced nutritional stress and pathogen interference in wolbachia-infected aedes aegypti. | the pathogen interference phenotype greatly restricts infection with dengue virus (denv) and other pathogens in wolbachia-infected aedes aegypti, and is a vital component of wolbachia-based mosquito control. critically, the phenotype's causal mechanism is complex and poorly understood, with recent evidence suggesting that the cause may be species specific. to better understand this important phenotype, we investigated the role of diet-induced nutritional stress on interference against denv and t ... | 2016 | 27893736 |
| cross-mating compatibility and competitiveness among aedes albopictus strains from distinct geographic origins - implications for future application of sit programs in the south west indian ocean islands. | the production of large numbers of males needed for a sustainable sterile insect technique (sit) control program requires significant developmental and operational costs. this may constitute a significant economic barrier to the installation of large scale rearing facilities in countries that are undergoing a transition from being largely dependent on insecticide use to be in a position to integrate the sit against aedes albopictus. alternative options available for those countries could be to r ... | 2016 | 27806056 |
| recent chikungunya virus infection in 2 travelers returning from mogadishu, somalia, to italy, 2016. | 2016 | 27513985 | |
| chikungunya virus infection: an update on joint manifestations and management. | the advent of sophisticated diagnostics has enabled the discovery of previously unknown arthropod-borne viruses like chikungunya. this infection has become increasingly prevalent in the last 10 years across the indian ocean and has been brought to media attention by a recent outbreak in the caribbean. the outbreak has been aided by a drastic rise in air travel, allowing infected individuals to transport the virus to previously unaffected regions. in addition, a recently documented viral mutation ... | 2016 | 27824550 |
| in silico study on anti-chikungunya virus activity of hesperetin. | the re-emerging, aedes spp. transmitted chikungunya virus (chikv) has recently caused large outbreaks in a wide geographical distribution of the world including countries in europe and america. though fatalities associated with this self-remitting disease were rarely reported, quality of patients' lives have been severely diminished by polyarthralgia recurrence. neither effective antiviral treatment nor vaccines are available for chikv. our previous in vitro screening showed that hesperetin, a b ... | 2016 | 27812412 |
| use of household cluster investigations to identify factors associated with chikungunya virus infection and frequency of case reporting in puerto rico. | chikungunya virus (chikv) is transmitted by aedes species mosquitoes and is the cause of an acute febrile illness characterized by potentially debilitating arthralgia. after emerging in the caribbean in late 2013, the first locally-acquired case reported to public health authorities in puerto rico occurred in may 2014. during june-august 2014, household-based cluster investigations were conducted to identify factors associated with infection, development of disease, and case reporting. | 2016 | 27764085 |
| chikungunya virus transmission between aedes albopictus and laboratory mice. | chikungunya virus (chikv) is a mosquito-borne alphavirus associated with epidemics of acute and chronic arthritic disease in humans. aedes albopictus has emerged as an important new natural vector for chikv transmission; however, mouse models for studying transmission have not been developed. | 2016 | 27760560 |
| mapping of courses on vector biology and vector-borne diseases systems: time for a worldwide effort. | major emergency efforts are being mounted for each vector-borne disease epidemiological crisis anew, while knowledge about the biology of arthropods vectors is dwindling slowly but continuously, as is the number of field entomologists. the discrepancy between the rates of production of knowledge and its use and need for solving crises is widening, in particular due to the highly differing time spans of the two concurrent processes. a worldwide web based search using multiple key words and search ... | 2016 | 27759770 |
| chikungunya virus in febrile humans and aedes aegypti mosquitoes, yucatan, mexico. | chikungunya virus (chikv) was isolated from 12 febrile humans in yucatan, mexico, in 2015. one patient was co-infected with dengue virus type 1. two additional chikv isolates were obtained from aedes aegypti mosquitoes collected in the homes of patients. phylogenetic analysis showed that the chikv isolates belong to the asian lineage. | 2016 | 27347760 |
| climate change influences potential distribution of infected aedes aegypti co-occurrence with dengue epidemics risk areas in tanzania. | dengue is the second most important vector-borne disease of humans globally after malaria. incidence of dengue infections has dramatically increased recently, potentially due to changing climate. climate projections models predict increases in average annual temperature, precipitation and extreme events in the future. the objective of this study was to assess the effect of changing climate on distribution of dengue vectors in relation to epidemic risk areas in tanzania. | 2016 | 27681327 |
| culex quinquefasciatus from rio de janeiro is not competent to transmit the local zika virus. | the americas have suffered a dramatic epidemic of zika since may in 2015, when zika virus (zikv) was first detected in brazil. mosquitoes belonging to subgenus stegomyia of aedes, particularly aedes aegypti, are considered the primary vectors of zikv. however, the rapid spread of the virus across the continent raised several concerns about the transmission dynamics, especially about potential mosquito vectors. the purpose of this work was to assess the vector competence of the house mosquito cul ... | 2016 | 27598421 |
| knowledge and use of prevention measures for chikungunya virus among visitors - virgin islands national park, 2015. | in june 2014, the mosquito-borne chikungunya virus (chikv) emerged in the u.s. virgin islands (usvi), a location where tourists comprise the majority of the population during peak season (january-april). limited information is available concerning visitors' chikv awareness and prevention measures. | 2016 | 27597388 |
| genetic evidence of expansion by passive transport of aedes (stegomyia) aegypti in eastern argentina. | 2016 | 27583521 | |
| zika virus, a new threat for europe? | since its emergence in 2007 in micronesia and polynesia, the arthropod-borne flavivirus zika virus (zikv) has spread in the americas and the caribbean, following first detection in brazil in may 2015. the risk of zikv emergence in europe increases as imported cases are repeatedly reported. together with chikungunya virus (chikv) and dengue virus (denv), zikv is transmitted by aedes mosquitoes. any countries where these mosquitoes are present could be potential sites for future zikv outbreak. we ... | 2016 | 27505002 |
| expression and characterization of yeast derived chikungunya virus like particles (chik-vlps) and its evaluation as a potential vaccine candidate. | chikungunya virus (chikv) has emerged as a global health concern due to its recent spread in both old and new world. so far, no chikv specific drug or vaccine is licensed for human use. in this study, we report production of chikungunya virus like particles (chik-vlps) using novel yeast expression system (pichia pastoris) and its evaluation as vaccine candidate. the gene encoding structural polyprotein of chikv from a recent epidemic strain was cloned into yeast expression system. the multicopy ... | 2016 | 27399001 |
| annotation of the asian citrus psyllid genome reveals a reduced innate immune system. | citrus production worldwide is currently facing significant losses due to citrus greening disease, also known as huanglongbing. the citrus greening bacteria, candidatus liberibacter asiaticus (clas), is a persistent propagative pathogen transmitted by the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae). hemipterans characterized to date lack a number of insect immune genes, including those associated with the imd pathway targeting gram-negative bacteria. the d. citri draft ... | 2016 | 27965582 |
| disruption of phenylalanine hydroxylase reduces adult lifespan and fecundity, and impairs embryonic development in parthenogenetic pea aphids. | phenylalanine hydroxylase (pah) is a key tyrosine-biosynthetic enzyme involved in neurological and melanin-associated physiological processes. despite extensive investigations in holometabolous insects, a pah contribution to insect embryonic development has never been demonstrated. here, we have characterized, for the first time, the pah gene in a hemimetabolous insect, the aphid acyrthosiphon pisum. phylogenetic and sequence analyses confirmed that appah is closely related to metazoan pah, exhi ... | 2016 | 27694983 |
| convergent evolution of trna gene targeting preferences in compact genomes. | in gene-dense genomes, mobile elements are confronted with highly selective pressure to amplify without causing excessive damage to the host. the targeting of trna genes as potentially safe integration sites has been developed by retrotransposons in various organisms such as the social amoeba dictyostelium discoideum and the yeast saccharomyces cerevisiae. in d. discoideum, trna gene-targeting retrotransposons have expanded to approximately 3 % of the genome. recently obtained genome sequences o ... | 2016 | 27583033 |
| small rna regulators of plant-hemipteran interactions: micromanagers with versatile roles. | non-coding small rnas (srnas) in plants have important roles in regulating biological processes, including development, reproduction, and stress responses. recent research indicates significant roles for srna-mediated gene silencing during plant-hemipteran interactions that involve all three of these biological processes. plant responses to hemipteran feeding are determined by changes in the host transcriptome that appear to be fine-tuned by srnas. the role of srna in plant defense responses is ... | 2016 | 27625654 |
| novel insights into insect-microbe interactions-role of epigenomics and small rnas. | it has become increasingly clear that microbes form close associations with the vast majority of animal species, especially insects. in fact, an array of diverse microbes is known to form shared metabolic pathways with their insect hosts. a growing area of research in insect-microbe interactions, notably for hemipteran insects and their mutualistic symbionts, is to elucidate the regulation of this inter-domain metabolism. this review examines two new emerging mechanisms of gene regulation and th ... | 2016 | 27540386 |
| heat sensitivity of wmel wolbachia during aedes aegypti development. | the wmel strain of wolbachia bacteria is known to prevent dengue and zika virus transmission in the mosquito vector aedes aegypti. accordingly, the release of wmel-infected a. aegypti in endemic regions has been recommended by the world health organization as a potential strategy for controlling dengue and zika outbreaks. however, the utility of this approach could be limited if high temperatures in the aquatic habitats where a. aegypti develop are detrimental to wolbachia. we exposed wmel-infec ... | 2016 | 27459519 |
| de novo transcriptome assembly of the grapevine phylloxera allows identification of genes differentially expressed between leaf- and root-feeding forms. | grapevine phylloxera, an insect related to true aphids, is a major historic pest of viticulture only controlled through the selection of resistant rootstocks or through quarantine regulations where grapevine is cultivated own-rooted. transcriptomic data could help understand the bases of its original life-traits, including a striking case of polyphenism, with forms feeding on roots and forms feeding in leaf-galls. comparisons with true aphids (for which complete genomes have been sequenced) shou ... | 2016 | 26968158 |
| detection of dengue, west nile virus, rickettsiosis and leptospirosis by a new real-time pcr strategy. | dengue virus (denv) infection causes sudden fever along with several nonspecific signs and symptoms and in severe cases, death. denv is transmitted to people by aedes aegypti and ae. albopictus mosquitoes, whose populations increase during rainy season. west nile virus (wnv), rickettsia spp. and leptospira spp. are fever-causing pathogens that share many of the initial symptoms of denv infection and also thrive in the rainy season. outbreaks in some regions may be due to any of these pathogens t ... | 2016 | 27350908 |
| zika virus: an update on epidemiology, pathology, molecular biology, and animal model. | zika virus (zikv) was first described in 1947, and became a health emergency problem in 2016 when its association with fetal microcephaly cases was confirmed by centers for disease control and prevention (cdc) in the united states. to date, zikv infection has been documented in 66 countries. zikv is recognized as a neurotropic virus and numerous diseases manifested in multiple neurological disorders have been described, mainly in countries that have been exposed to zikv after the 2007 outbreak i ... | 2016 | 27124623 |
| magnitude of neglected tropical diseases in indonesia at postmillennium development goals era. | the world will enter the postmillennium development goals 2015 era. the achievements of the millennium development goals (mdgs) as a global development target need to be evaluated. a sustainable new reasonable target is important for neglected tropical diseases (ntd) elimination in indonesia. this review describes the ntd situation in indonesia and highlights problems beneath the ntd transmission. multidisciplinary approach is a promising strategy to help the marginalized people. | 2016 | 27190525 |
| the untapped cell biology of neglected tropical diseases. | the world health organization lists a constellation of 17 tropical diseases that afflict approximately one in six individuals on the planet and, until recently, few resources have been devoted to the treatment and eradication of those diseases. they are often referred to as the diseases of the "bottom billion," because they are most prevalent among the poorest individuals in impoverished tropical nations. however, the few studies that have been performed reveal an extraordinary world of molecula ... | 2016 | 26915691 |
| structure and functional diversity of gcn5-related n-acetyltransferases (gnat). | general control non-repressible 5 (gcn5)-related n-acetyltransferases (gnat) catalyze the transfer of an acyl moiety from acyl coenzyme a (acyl-coa) to a diverse group of substrates and are widely distributed in all domains of life. this review of the currently available data acquired on gnat enzymes by a combination of structural, mutagenesis and kinetic methods summarizes the key similarities and differences between several distinctly different families within the gnat superfamily, with an emp ... | 2016 | 27367672 |
| overview of zika virus (zikv) infection in regards to the brazilian epidemic. | zika virus (zikv), a mosquito-borne flavivirus, belongs to the flaviviridae family, genus flavivirus. zikv was initially isolated in 1947 from a sentinel monkey in the zika forest, uganda. little clinical importance was attributed to zikv, once only few symptomatic cases were reported in some african and southeast asiatic countries. this situation changed in 2007, when a large outbreak was registered on the yap island, micronesia, caused by the asian zikv lineage. between 2013 and 2014, zikv spr ... | 2016 | 27143174 |
| zika: the origin and spread of a mosquito-borne virus. | to describe the temporal and geographical distribution of zika virus infection and associated neurological disorders, from 1947 to 1 february 2016, when zika became a public health emergency of international concern (pheic). | 2016 | 27708473 |
| persistent infection by wolbachia walbb has no effect on composition of the gut microbiota in adult female anopheles stephensi. | the bacteria in the midgut of anopheles stephensi adult females from laboratory colonies were studied by sequencing the v4 region of 16s rrna genes, with respect to three experimental factors: stable or cured wolbachia infection; sugar or blood diet; and age. proteobacteria and bacteroidetes dominated the community [>90% of operational taxonomic units (otus)]; most taxa were in the classes flavobacteriia, gammaproteobacteria, and alphaproteobacteria, and were assigned to elizabethkingia (46.9%), ... | 2016 | 27708633 |
| functional integration of the circulatory, immune, and respiratory systems in mosquito larvae: pathogen killing in the hemocyte-rich tracheal tufts. | as both larvae and adults, mosquitoes encounter a barrage of immune insults, ranging from microbe-rich communities in larval habitats to ingested blood-borne pathogens in adult blood meals. given that mosquito adults have evolved an efficient means of eliminating infections in their hemocoel (body cavity) via the coordinated action of their immune and circulatory systems, the goal of the present study was to determine whether such functional integration is also present in larvae. | 2016 | 27643786 |
| mosquito c-type lectins maintain gut microbiome homeostasis. | the long-term evolutionary interaction between the host immune system and symbiotic bacteria determines their cooperative rather than antagonistic relationship. it is known that commensal bacteria have evolved a number of mechanisms to manipulate the mammalian host immune system and maintain homeostasis. however, the strategies employed by the microbiome to overcome host immune responses in invertebrates still remain to be understood. here, we report that the gut microbiome in mosquitoes utilize ... | 2016 | 27170846 |
| open-air biowarfare testing and the evolution of values. | the united states and the united kingdom ended outdoor biological warfare testing in populated areas nearly half a century ago. yet, the conduct, health effects, and propriety of those tests remain controversial. the varied views reflect the limits of currently available test information and evolving societal values on research involving human subjects. western political culture has changed since the early days of the american and british testing programs. people have become less reluctant to qu ... | 2016 | 27564984 |
| symbiosis with francisella tularensis provides resistance to pathogens in the silkworm. | francisella tularensis, the causative agent of tularemia, is a highly virulent facultative intracellular pathogen found in a wide range of animals, including arthropods, and environments. this bacterium has been known for over 100 years, but the lifestyle of f. tularensis in natural reservoirs remains largely unknown. thus, we established a novel natural host model for f. tularensis using the silkworm (bombyx mori), which is an insect model for infection by pathogens. f. tularensis established a ... | 2016 | 27507264 |
| advances and limitations of disease biogeography using ecological niche modeling. | mapping disease transmission risk is crucial in public and animal health for evidence based decision-making. ecology and epidemiology are highly related disciplines that may contribute to improvements in mapping disease, which can be used to answer health related questions. ecological niche modeling is increasingly used for understanding the biogeography of diseases in plants, animals, and humans. however, epidemiological applications of niche modeling approaches for disease mapping can fail to ... | 2016 | 27547199 |