Publications

effects of preexposure to deet on the downstream blood-feeding behaviors of aedes aegypti (diptera: culicidae) mosquitoes.	mosquito behavior is heavily influenced by the chemical molecules in the environment. this knowledge can be used to modify insect behaviors; particularly to reduce vector-host contact as a powerful method for disease prevention. n,n-diethyl-meta-toluamide (deet) is the most widely used insect repellent in the market and an excellent example of a chemical that has been used to modify insect behavior for disease prevention. however, genetic insensitivity and habituation in aedes aegypti (l.) mosqu ...	2016	27288690
somatostatin signaling system as an ancestral mechanism: myoregulatory activity of an allatostatin-c peptide in hydra.	the coordination of physiological processes requires precise communication between cells. cellular interactions allow cells to be functionally related, facilitating the maintaining of homeostasis. neuropeptides functioning as intercellular signals are widely distributed in metazoa. it is assumed that neuropeptides were the first intercellular transmitters, appearing early during the evolution. in cnidarians, neuropeptides are mainly involved in neurotransmission, acting directly or indirectly on ...	2016	27288244
chemical composition and biological activity of essential oils from different species of piper from panama.	the chemical composition of leaf essential oils from 11 species of piper from panama was analyzed by a combination gc-fid and gc-ms procedures. six of them had sesquiterpene hydrocarbons as major constituents, three were characterized by monoterpene hydrocarbons, one by a diterpene, and one by a phenylpropanoid, dillapiole. the main components identified in each species were: cembratrienol (25.4 %) in piper augustum; β-pinene (26.6 %) in piper corrugatum; α-pinene (19.4 %) in piper curtispicum; ...	2016	27286333
reported distribution of aedes (stegomyia) aegypti and aedes (stegomyia) albopictus in the united states, 1995-2016 (diptera: culicidae).	aedes (stegomyia) aegypti (l.) and aedes (stegomyia) albopictus (skuse) transmit arboviruses that are increasing threats to human health in the americas, particularly dengue, chikungunya, and zika viruses. epidemics of the associated arboviral diseases have been limited to south and central america, mexico, and the caribbean in the western hemisphere, with only minor localized outbreaks in the united states. nevertheless, accurate and up-to-date information for the geographical ranges of ae. aeg ...	2016	27282817
semi-field evaluation of metofluthrin-impregnated nets on host-seeking aedes aegypti and anopheles dirus.	the efficacy of a metofluthrin-impregnated net (min) known as the "mushikonazu" on the house entry behavior of female aedes aegypti and anopheles dirus mosquitoes was evaluated using a semi-field 50-m tunnel setup. while the min is labeled for the control of chironomids and moth flies, this study determined the feasibility of using the device, given its current construction and metofluthrin formulation, as a spatial repellent against mosquitoes. sentinel and cone bioassays were used to determine ...	2016	27280351
new candidates for plant-based repellents against aedes aegypti.	based on an ethnobotanical study on use for plant species against mosquito bites in the kota tinggi district, johor state, malaysia, 3 plants selected for study, citrus aurantifolia (leaves), citrus grandis (fruit peel), and alpinia galanga (rhizome), were extracted using hydrodistillation to produce essential oils. these essential oils were then formulated as a lotion using a microencapsulation process and then tested for their repellent effect against aedes aegypti. n,n-diethyl-m-toluamide (de ...	2016	27280349
toxicology of potassium channel-directed compounds in mosquitoes.	potential targets for new vector control insecticides are nerve and muscle potassium channels. in this study, the activities of known potassium channel blockers (4-aminopyridine, quinidine, and tetraethylammonium) and the insecticide propoxur were compared to three experimental catechols and several other compounds against anopheles gambiae and aedes aegypti mosquitoes. experimental catechol 1 was the most toxic experimental compound in all of the mortality assays conducted, but was at least 100 ...	2016	27262624
evaluation of alternative killing agents for aedes aegypti (diptera: culicidae) in the gravid aedes trap (gat).	the gravid aedes trap (gat) uses visual and olfactory cues to attract gravid aedes aegypti (l.) that are then captured when knocked down by a residual pyrethroid surface spray. however, the use of surface sprays can be compromised by poor availability of the spray and pesticide resistance in the target mosquito. we investigated several "alternative" insecticide and insecticide-free killing agents for use in the gat. this included long-lasting insecticide-impregnated nets (llins), vapor-active sy ...	2016	27247350
species composition and habitat characterization of mosquito (diptera: culicidae) larvae in semi-urban areas of dhaka, bangladesh.	mosquito larvae are purely aquatic and develop in water bodies, the type of which is more or less specific to each species. therefore, a study was carried out to identify the habitat characters of different mosquito species along with their species composition in semi-urban area of dhaka in bangladesh during the month of may and june 2012. a total of 6088 mosquito larvae belonging to 12 species (aedes aegypti, aedes albopictus, anopheles barbirostris, anopheles peditaeniatus, anopheles vagus, cu ...	2016	27241953
effects of temperature on the life cycle, expansion, and dispersion of aedes aegypti (diptera: culicidae) in three cities in paraiba, brazil.	the mosquito aedes aegypti is the primary vector of dengue and is common throughout tropical and subtropical regions. its distribution is modulated by environmental factors, such as temperature. this study aimed to evaluate the influence of temperature on the life cycle and expansion of ae. aegypti populations in the cities of campina grande, joão pessoa, and patos. samples of ae. aegypti were collected in the three cities and raised in the laboratory. we assessed the life cycles of the three ae ...	2016	27232118
the effect of wolbachia on dengue outbreaks when dengue is repeatedly introduced.	use of the wolbachia bacterium is a proposed new strategy to reduce dengue transmission, which results in around 390 million individuals infected annually. in places with strong variations in climatic conditions such as temperature and rainfall, dengue epidemics generally occur only at a certain time of the year. where dengue is not endemic, the time of year in which imported cases enter the population plays a crucial role in determining the likelihood of outbreak occurrence. we use a mathematic ...	2016	27217229
yellow fever vaccination status and safety in hemodialysis patients.	the adverse effects of yellow fever (yf) vaccine in dialysis patients are not well known. there is concern about the risks and benefits of the vaccine in immunocompromised patients living in endemic areas, particularly given the risk of resurgence of urban yf with the spread of aedes aegypti mosquitoes. the purpose of this study was to assess the coverage and safety of yf vaccine in chronic dialysis patients.	2016	27208638
urban climate versus global climate change-what makes the difference for dengue?	the expansion in the geographical distribution of vector-borne diseases is a much emphasized consequence of climate change, as are the consequences of urbanization for diseases that are already endemic, which may be even more important for public health. in this paper, we focus on dengue, the most widespread urban vector-borne disease. largely urban with a tropical/subtropical distribution and vectored by a domesticated mosquito, aedes aegypti, dengue poses a serious public health threat. temper ...	2016	27197685
entomological assessment of yellow fever-epidemic risk indices in benue state, nigeria, 2010-2011.	yellow fever (yf) is a vector-borne disease affecting humans and non-human primates in tropical areas. in the past, there have been pockets of yf outbreaks in nigeria that resulted in preventable deaths. surveillance efforts towards avoiding another outbreak have been put in place with the aim of early detection and control. however, risk indices relating to the density of immature yf-mosquito vectors are given little consideration even though it is the first step in curbing a possible outbreak. ...	2016	27189925
functional and immunohistochemical characterization of cceae3a, a carboxylesterase associated with temephos resistance in the major arbovirus vectors aedes aegypti and ae. albopictus.	temephos is a major organophosphate (op) larvicide that has been used extensively for the control of aedes albopictus and aedes aegypti, the major vectors for viral diseases, such as dengue fever, zika and chikungunya. resistance to temephos has been recently detected and associated with the upregulation of carboxylesterases (cces) through gene amplification, in both species. here, we expressed the cceae3a genes which showed the most striking up-regulation in resistant aedes strains, using the b ...	2016	27180726
dsrna-mediated targeting of ribosomal transcripts rps6 and rpl26 induces long-lasting and significant reductions in fecundity of the vector aedes aegypti.	ribosomal transcripts produce critical proteins that are involved in most cellular production processes. targeting ribosomal transcripts has produced mortality in mites and ticks but the effect of ribosomal transcript knockdown has not been thoroughly examined in mosquitoes. we examine the effects of triggers targeting four ribosomal proteins (rp) transcripts. although no significant mortality was observed after dsrna microinjection and subsequent blood feeding, significant contrasts were observ ...	2016	27180677
toxicological evaluation of essential oil from the leaves of croton tetradenius (euphorbiaceae) on aedes aegypti and mus musculus.	for control of aedes aegypti, the main vector of dengue, botanical insecticides can be a viable alternative. herein, we evaluated the chemical composition and insecticidal activity of the essential oils of the leaves of croton tetradenius on ae. aegypti larvae and adults. we also evaluated the acute toxicity in mus musculus. the essential oil chemical analysis was performed using chromatography coupled with mass spectrometry and flame ionization detection. female mice were used for assessing tox ...	2016	27169864
yellow fever cases in asia: primed for an epidemic.	there is currently an emerging outbreak of yellow fever in angola. cases in infected travellers have been reported in a number of other african countries, as well as in china, representing the first ever documented cases of yellow fever in asia. there is a large chinese workforce in angola, many of whom may be unvaccinated, increasing the risk of ongoing importation of yellow fever into asia via busy commercial airline routes. large parts of the region are hyperendemic for the related flavivirus ...	2016	27156836
δ-cadinene, calarene and .δ-4-carene from kadsura heteroclita essential oil as novel larvicides against malaria, dengue and filariasis mosquitoes.	mosquitoes (diptera: culicidae) are major vectors of important pathogens and parasites. malaria, dengue fever, yellow fever, filariasis, schistosomiasis and japanese encephalitis cause millions of deaths every year. mosquito control is being challenging due to the development of pesticide resistance and negative environmental concerns. in this scenario, plants employed in traditional asian medicine may be alternative sources of newer and effective mosquitocides. in this research, we evaluated th ...	2016	27151483
impact of inter- and intra-specific competition among larvae on larval, adult, and life-table traits of aedes aegypti and aedes albopictus females.	few studies have taken a comprehensive approach of measuring the impact of inter- and intra-specific larval competition on adult mosquito traits. in this study, the impact of competition aedes aegypti and a. albopictus was quantified over the entire life of a cohort.competitive treatments affected hatch-to-adult survivorship and development time to adulthood of females for both species, but affected median wing length of females only for a. albopictus. competitive treatments had no significant e ...	2016	27141149
multiple qtl determine dorsal abdominal scale patterns in the mosquito aedes aegypti.	the mosquito, aedes aegypti (l.) originated in sub-saharan africa as a dark form sylvan species (a. aegypti formosus). evolution of a. aegypti aegypti type form as a human commensal facilitated its colonization of most semitropical and tropical areas. we investigated the genetic basis for abdominal white scale presence that represents the diagnostic for sylvan a. aegypti formosus (scales absent), from type form (scales present) and a. aegypti queenslandensis form (dense scaling). we performed qu ...	2016	27130203
insights into the evolution of chemoreceptor genes superfamily in tyrophagus putrescentiae (acari: acaridae).	all living organisms, including animals, plants, fungi, and bacteria, use the olfactory system to recognize chemicals or pheromone from their environment. insects detect a volatile substance using odorant receptors (ors) or gustatory receptors (grs) and ionotropic receptors (irs). the gene families of the olfactory system in acari are still not clear. in this study, we identified seven ors, one gr, and five irs from the transcriptome of the storage mite, tyrophagus putrescentiae schrank. no olfa ...	2016	27113112
detection and distribution of v1016ikdr mutation in the voltage-gated sodium channel gene in aedes aegypti (diptera: culicidae) populations from sergipe state, northeast brazil.	aedes aegypti (l.) resistance to pyrethroids was recorded in brazil few years after its introduction as the adulticide in the national dengue control program campaigns. altered susceptibility to pyrethroids had been reported in the state of sergipe, northeast brazil, through biological assays, even before its use against ae. aegypti in the state. metabolic and target-site resistance mechanisms were also revealed in samples from aracaju, the capital of sergipe. herein, we investigated the presenc ...	2016	27113110
recent changes in the local distribution of aedes aegypti (diptera: culicidae) in south florida, usa.	disease transmission is directly tied to the spatial distribution of disease vectors. the distribution of aedes aegypti (l.) in the united states has diminished since the introduction of aedes albopictus (skuse) in the 1980s. however, ae. aegypti persists in some urban areas, particularly in south florida. the pattern of habitat segregation of these two species is well documented, but the consistency of this phenomenon over time is unknown. to examine the dynamics of the local distributions of t ...	2016	27113103
characteristics and efficacy of three commercial handheld thermal foggers with pyrethroid insecticides against three species of mosquitoes.	the field study's objectives were to compare the physical characteristics as well as efficacy with multiple insecticides for bonide(®) fog rx insect fogger, black flag(®) propane insect fogger, and burgess(®) outdoor propane insect fogger. evaluations were conducted with 7 machine chemical combinations, 3 depths of spray, and 3 species of laboratory-reared mosquitoes, anopheles quadrimaculatus, culex quinquefasciatus, and aedes aegypti. combinations of these factors were analyzed in conjunction ...	2016	27105215
age-stage, two-sex life table characteristics of aedes albopictus and aedes aegypti in penang island, malaysia.	the life table developmental attributes of laboratory colonies of wild strains of aedes albopictus and aedes aegypti were analyzed and compared based on the age-stage, two-sex life table. findings inclusive in this study are: adult preoviposition periods, total preoviposition period, mean intrinsic rate of increase (r), mean finite rate of increase (λ), net reproductive rates (r0), and mean generation time (t). the total preadult development time was 9.47 days for ae. albopictus and 8.76 days fo ...	2016	27105211
comparative analysis of midgut bacterial communities in three aedine mosquito species from dengue-endemic and non-endemic areas of rajasthan, india.	dengue viruses are transmitted to humans through the bites of infected female aedine mosquitoes. differences in the composition and structure of bacterial communities in the midguts of mosquitoes may affect the vector's ability to transmit the disease. to investigate and analyse the role of midgut bacterial communities in viral transmission, midgut bacteria from three species, namely stegomyia aegypti (= aedes aegypti), fredwardsius vittatus (= aedes vittatus) and stegomyia albopicta (= aedes al ...	2016	27094337
profile distribution of juvenile aedes species in an urban area of malaysia.	dengue fever (df) is an urban vector-borne disease transmitted by aedes aegypti and aedes albopictus. both species deposit their eggs in favorable breeding sites either in natural or artificial containers. an understanding of their habitat characteristics is crucial in curbing df outbreaks	2016	27076510
homology modeling and molecular docking studies of drosophila and aedes sex peptide receptors.	the drosophila melanogaster sex peptide receptor (drmspr), which is a g protein-coupled receptor (gpcr), is known as the specific receptor for sex peptide (sp). it is responsible for the reproductive behavior in the drosophila model system; in particular, it is involved in the post-mating responses such as the increase in egg-laying ability and decrease in receptivity in females. in a previous study, we discovered a small molecule agonist of drmspr for the first time, which could not, however, a ...	2016	27060892
functional validation of the carbon dioxide receptor in labial palps of helicoverpa armigera moths.	adult moths possess an organ in their labial palps, the labial-palp pit organ, which is specialized for sensing carbon dioxide (co2). they use co2 as a cue to detect healthy plants and find food or lay eggs on them. the molecular bases of the co2 receptor in drosophila melanogaster and aedes aegypti have been reported, but the molecular mechanisms of the co2 receptor in lepidoptera remains elusive. in this study, we first re-examined three putative helicoverpa armigera co2 gustatory receptor gen ...	2016	27060445
molecular cloning, sequence analysis and developmental stage expression of a putative septin gene fragment from aedes albopictus (diptera: culicidae).	septins belong to gtpases that are involved in vital cellular activities, including cytokinesis. although present in many organisms, they are yet to be isolated from aedes albopictus. this study reports for the first time on a serendipitous isolation of a partial septin sequence from ae. albopictus and its developmental expression profile. the ae. albopictus partial septin sequence contains 591 nucleotides encoding 197 amino acids. it shares homology with several insect septin genes and has a cl ...	2016	27059016
biological and chemical characterization of a new larvicide ovitrap made of plastic with pyriproxyfen incorporated for aedes aegypti (diptera: culicidae) control.	aedes aegypti (l.) is a species of international concern because of its ability to transmit serious human arboviral diseases including yellow fever, dengue, and chikungunya, which have spread to all continents. ovitraps are containers constructed to imitate aedes' natural breeding sites and have been used for many decades as a sensitive and inexpensive surveillance tool for detecting the presence of container-inhabiting mosquitoes. in addition to their value for vector surveillance, various ovit ...	2016	27052297
frequency of the val1016ile mutation on the kdr gene in aedes aegypti (diptera: culicidae) in south brazil.	recently, the number of aedes aegypti foci has increased in west of santa catarina, south brazil, which has increased concern regarding mosquito-borne disease outbreaks such as dengue fever, zika virus, and chikungunya. therefore, it is important to monitor genetic resistance to insecticides through "knockdown resistance". homozygosity (ile/ile) at position 1016 in the coding region of a voltage-dependent sodium channel gene (nav) may induce resistance to pyrethroid insecticides. we evaluated th ...	2016	27886338
one year after the zika virus outbreak in brazil: from hypotheses to evidence.	zika virusis an arbovirus of the flaviviridae family with two major strains, an asian and an african strain. the main vectors involved in the transmission of zika virus are the aedes aegypti and aedes albopictus mosquitoes. despite its identification, discovered in 1947 in the zika forest in uganda, only isolated and sporadic occurrences of human infection were reported within a largely asymptomatic proportion of individuals. the first reported outbreak occurred in 2007 in the yap island, which ...	2016	27812646
sex, mosquitoes and epidemics: an evaluation of zika disease dynamics.	since the first major outbreak reported on the island yap in 2007, the zika virus spread has alerted the scientific community worldwide. zika is an arbovirus transmitted by aedes mosquitoes; particularly in central and south america, the main vector is the same mosquito that transmits dengue and chikungunya, aedes aegypti. seeking to understand the dynamics of spread of the zika, in this paper, three mathematical models are presented, in which vector transmission of the virus, sexual contact tra ...	2016	27743310
alkaloids with activity against the zika virus vector aedes aegypti (l.)-crinsarnine and sarniensinol, two new crinine and mesembrine type alkaloids isolated from the south african plant nerine sarniensis.	two new amaryllidaceae alkaloids, belonging to the mesembrine- and crinine-types, named crinsarnine (1) and sarniensinol (2), were isolated from the dried bulbs of nerine sarniensis together with bowdensine (3), sarniensine (4), hippadine (5) and 1-o-acetyl-lycorine (6). crinsarnine (1) and sarniensinol (2) were characterized using spectroscopic and chiroptical methods as (1s,2s,4ar,10bs)-2,7-dimethoxy-1,2,3,4,4a,6-hexahydro-5,11b-ethano[1,3]dioxolo-[4,5-j]phenanthridin-1-yl acetate and (6-(3ar, ...	2016	27801807
the correlation between dengue incidence and diurnal ranges of temperature of colombo district, sri lanka 2005-2014.	background meteorological factors affect dengue transmission. mechanisms of the way in which different diurnal temperatures, ranging around different mean temperatures, influence dengue transmission were published after 2011. objective we endeavored to determine the correlation between dengue incidence and diurnal temperature ranges (dtrs) in colombo district, sri lanka, and to explore the possibilities of using our findings to improve control of dengue. design we calculated the weekly dengue in ...	2016	28156991
acoustic control of mosquito larvae in artificial drinking water containers.	emerging technology designed to kill mosquito larvae with sound waves may present a nonchemical and nonbiological alternative to reduce larval populations of key medically important mosquito species such as aedes aegypti in containers or catchments of water. these devices could benefit integrated vector management programs facing public resistance to the use of chemical or biological larvicides in stored drinking water. in this study we investigate the efficacy of a larvasonic sd-mini acoustic l ...	2016	28206869
effect of photoperiod on permethrin resistance in aedes aegypti.	living organisms have been exposed to light-dark cycles that allowed them to adapt to different ecological niches. circadian cycles affect hormone release, metabolism, and response to xenobiotic compounds. current studies have shown that insect susceptibility to toxic agents depends on circadian cycles, mainly because the biochemical processes involved in detoxification and responses to oxidative stress are modulated by this process. the goal of this study was to determine the effect of photoper ...	2016	28206856
toxicity and physiological actions of carbonic anhydrase inhibitors to aedes aegypti and drosophila melanogaster.	the physiological role of carbonic anhydrases in ph and ion regulation is crucial to insect survival. we examined the toxic and neurophysiological effects of five carbonic anhydrase inhibitors (cais) against aedes aegypti. the 24 h larvicidal toxicities followed this rank order of potency: dichlorphenamide > methazolamide > acetazolamide = brinzolamide = dorzolamide. larvicidal activity increased modestly in longer exposures, and affected larvae showed attenuated responses to probing without ove ...	2016	28025488
exotic mosquito threats require strategic surveillance and response planning.	mosquito-borne diseases caused by endemic pathogens such as ross river, barmah forest and murray valley encephalitis viruses are an annual concern in new south wales (nsw), australia. more than a dozen mosquito species have been implicated in the transmission of these pathogens, with each mosquito occupying a specialised ecological niche that influences their habitat associations, host feeding preferences and the environmental drivers of their abundance. the nsw arbovirus surveillance and mosqui ...	2016	27997935
papular urticaria: a review of causal agents in colombia.	papular urticaria is a chronic allergic reaction induced by insect bites, which is common in the tropics. the objective of this review was to deepen on epidemiological and immunological aspects of this disease, focused on data published in latin american countries.we conducted a non-systematic review of the literature through electronic search on the epidemiology of papular urticaria, the entomological characteristics of the causative agents and associated immunological mechanisms.several report ...	2016	27992990
cocos nucifera coir-mediated green synthesis of pd nps and its investigation against larvae and agricultural pest.	in recent decades, several scientists focused their process towards nanoparticles synthesis by using various sustainable approaches. cocos nucifera (c. nucifera) was one of the versatile trees in tropical regions which also can act as a thrust quencher in all over the world. cocos nucifera coir was one of the waste by-products in all coconut-refining industries and with the help c. nucifera coir, palladium nanoparticles (pd nps) were synthesized. green-synthesized spherical-shape pd nps were ove ...	2016	27908201
larvicidal activity of green synthesized silver nanoparticles using excoecaria agallocha l. (euphorbiaceae) leaf extract against aedes aegypti.	green synthesis of silver nanoparticles (agnps) using plant extracts has been achieved by eco-friendly reducing and capping agents. the present study was conducted to evaluate the larvicidal efficacies of agnps synthesized using aqueous leaf extracts of excoecaria agallocha against dengue vector, aedes aegypti. the 3(rd) and 4(th) instar larvae of a. aegypti were exposed to various concentrations of aqueous extracts of e. agallocha, synthesized agnps and also crude solvent extracts (methanol and ...	2016	27906138
spatial pattern evolution of aedes aegypti breeding sites in an argentinean city without a dengue vector control programme.	the main objective of this study was to obtain and analyse the space-time dynamics of aedes aegypti breeding sites in clorinda city, formosa province, argentina coupled with landscape analysis using the maximum entropy approach in order to generate a dengue vector niche model. in urban areas, without vector control activities, 12 entomologic (larval) samplings were performed during three years (october 2011 to october 2014). the entomologic surveillance area represented 16,511 houses. predictive ...	2016	27903056
wolbachia suppresses cell fusing agent virus in mosquito cells.	the genus flavivirus contains a large number of positive-sense ssrna viruses. while some are transmitted by mosquitoes or other arthropods and are pathogenic to humans and animals (e.g. dengue and zika viruses), some are insect-specific and do not replicate in vertebrate cells. these are known as insect-specific flaviviruses (isfs). cell fusing agent virus (cfav) was the first described isf, which was detected in an aedes aegypti cell line, aag2. here, we investigated the effect of wolbachia, a ...	2016	27902358
fern-synthesized silver nanocrystals: towards a new class of mosquito oviposition deterrents?	mosquitoes act as vectors of devastating pathogens and parasites, representing a key threat for millions of humans and animals worldwide. eco-friendly control tools are urgently required. we proposed a novel method of fern-mediated biosynthesis of silver nanoparticles (agnp) using dicranopteris linearis, acting as a reducing and capping agent. agnp were characterized by uv-vis spectroscopy, fourier transform infrared (ftir) spectroscopy, scanning electron microscopy (sem), energy-dispersive x-ra ...	2016	27892872
methods and procedures used in aedes aegypti control in the successful campaign for yellow fever prophylaxis in rio de janeiro, brazil, in 1928 and 1929.	to review the challenges and procedures used in aedes aegypti control and the characteristics of the epidemics in the successful campaign, led by clementino fraga, for yellow fever control in rio de janeiro, brazil, in 1928 and 1929, before the vaccine (1937) and ddt (1947).	2016	27869977
incidence of dengue cases (2007-2013) and seasonal distribution of mosquitoes (diptera: culicidae) (2012-2013) in barreiras, bahia, brazil.	to describe seasonal mosquito distribution and the incidence of dengue cases in barreiras, bahia, brazil.	2016	27869967
[aedes aegypti control strategies: a review].	to describe the main strategies to control aedes aegypti, with emphasis on promising technological innovations for use in brazil.	2016	27869956
no magic bullet: citizenship and social participation in the control of aedes aegypti.		2016	27869933
[first report of the f1534c mutation associated with cross-resistance to ddt and pyrethroids in aedes aegypti from colombia].	the main strategy for the control of aedes aegypti, vector of dengue, chikungunya and zika viruses, is based on the use of insecticides to reduce its populations. however, their use has led to insect resistance to these chemicals. objective: to determine the presence of the f1534c mutation associated with cross-resistance to ddt and pyrethroids in a. aegypti in sincelejo, colombia. materials and methods: we studied nine specimens of a. aegypti that showed resistance to lambdacyhalothrin in bioas ...	2016	27869391
housing improvement: a novel paradigm for urban vector-borne disease control?		2016	27864518
epidemiological characteristics of yellow fever in brazil, 2000-2012.	this study aims to describe the epidemiological characteristics of yellow fever in brazil in the period 2000-2012.	2016	27861674
movement of aedes aegypti following a sugar meal and its implication in the development of control strategies in durán, ecuador.	we evaluated how the presence of sugar sources impacted the distribution of aedes aegypti in different habitats in durán, ecuador. land cover and normalized difference vegetation index maps were used to guide a random point sampling routine to select study grids (30 m × 30 m) in low vegetation (lv) and high vegetation (hv). five individual plants, at one home in the lv and hv grid, were treated with a different colored, non-attractive, 60% sucrose solution to determine mosquito feeding and movem ...	2016	27860016
evaluation of collection methods for culex quinquefasciatus, aedes aegypti, and aedes simpsoni in northeastern tanzania.	in east africa, significant morbidity and mortality are caused by infections spread by culex quinquefasciatus and aedes aegypti. sticky traps have been shown to be effective tools for sampling populations of aedes mosquitoes and have been found to catch cx. quinquefasciatus. thus, they could potentially be used to sample populations of this species. this study compared sticky ovitraps (so) and mosquitraps (mqt) with the cdc gravid trap (cdc-gt) for collection of culex and aedes mosquito populati ...	2016	27860009
evidence of the presence of the zika virus in mexico since early 2015.	to assess the possible circulation of zika virus (zikv) prior to the first documented case in mexico, we reanalyzed the stored samples from the states of veracruz and yucatán, which were originally collected to test for dengue (denv) and chikungunya (chikv) but were negative for these viruses despite the symptomatology. the samples were originally collected between the 30 and 46 epidemiological weeks (ew) when the zikv was not yet declared as a public health emergency of international concern (p ...	2016	27557815
an updated review of zika virus.	the current outbreak of zika virus (zikv) in south america is one of the most serious public health emergencies since the ebola outbreak of west africa [2014]. zikv belongs to the flaviviridae family and has two lineages (asian and african). the virus was first discovered in uganda [1947] and the first human infection was identified in nigeria [1952]. the current epidemic is the third of its type after that of yap island, micronesia [2007] and french polynesia [2013]. phylogenetic studies reveal ...	2016	27721110
zika virus disease cases - 50 states and the district of columbia, january 1-july 31, 2016.	zika virus is a mosquito-borne flavivirus primarily transmitted to humans by aedes aegypti mosquitoes (1). zika virus infections have also been documented through intrauterine transmission resulting in congenital infection; intrapartum transmission from a viremic mother to her newborn; sexual transmission; blood transfusion; and laboratory exposure (1-5). most zika virus infections are asymptomatic (1,6). clinical illness, when it occurs, is generally mild and characterized by acute onset of fev ...	2016	27631604
epidemiology, virology, and pathogenesis of the zika virus: from neglected tropical disease to a focal point of international attention.	over the past year, the zika virus, an arthropod-borne flavivirus, has transitioned from a relatively unknown tropical disease to the cause of a public health emergency. the zika virus is transmitted by the aedes species of mosquito as well as by sexual intercourse. although the symptoms of acute zika virus infection are usually mild and self-limited, it causes fetal microcephaly in pregnant women, and is associated with an increased risk of guillain-barré syndrome. the risk of microcephaly from ...	2016	27618294
single-step biological fabrication of colloidal silver nanoparticles using hugonia mystax: larvicidal potential against zika virus, dengue, and malaria vector mosquitoes.	mosquito control is facing key challenges, including outbreaks of new arbovirus threats. we proposed an eco-friendly synthesis of silver nanoparticles (agnps) employing a low-cost extract of hugonia mystax. agnps were specified by uv, xrd, ftir and edx spectroscopy, sem and tem. agnps were more toxic to anopheles stephensi, aedes aegypti, and culex quinquefasciatus larvae (lc50: 14.45, 15.86, and 17.46 μg/ml) if compared to aquatic biocontrol organisms gambusia affinis, diplonychus indicus, and ...	2016	27611658
acute toxicity and repellent activity of the origanum scabrum boiss. & heldr. (lamiaceae) essential oil against four mosquito vectors of public health importance and its biosafety on non-target aquatic organisms.	the recent outbreaks of dengue, chikungunya, and zika virus highlighted the pivotal importance of mosquito vector control in tropical and subtropical areas worldwide. however, mosquito control is facing hot challenges, mainly due to the rapid development of pesticide resistance in culicidae and the limited success of biocontrol programs on aedes mosquitoes. in this framework, screening botanicals for their mosquitocidal potential may offer effective and eco-friendly tools in the fight against mo ...	2016	27604128
culex species mosquitoes and zika virus.	recent reports of zika virus (zikv) isolates from culex species mosquitoes have resulted in concern regarding a lack of knowledge on the number of competent vector species for zikv transmission in the new world. although observations in the field have demonstrated that zikv isolation can be made from culex species mosquitoes, the detection of zikv in these mosquitoes is not proof of their involvement in a zikv transmission cycle. detection may be due to recent feeding on a viremic vertebrate, an ...	2016	27556838
what's in the literature?	the guillain-barré syndrome (gbs) is one of the few neuropathies well known to the general public, in part because of its association with swine flu vaccinations in 1976. gbs has again reached the general public with its possible association with zika virus. the virus, borne by infected aedes aegypti mosquitos, is being linked to birth defects when pregnant women are bitten and infected. there are early reports also linking gbs to zika infection, which could expose a wider range of infected peop ...	2016	27552392
contraceptive use among nonpregnant and postpartum women at risk for unintended pregnancy, and female high school students, in the context of zika preparedness - united states, 2011-2013 and 2015.	zika virus infection during pregnancy can cause congenital microcephaly and brain abnormalities (1,2). since 2015, zika virus has been spreading through much of the world health organization's region of the americas, including u.s. territories. zika virus is spread through the bite of aedes aegypti or aedes albopictus mosquitoes, by sex with an infected partner, or from a pregnant woman to her fetus during pregnancy.* cdc estimates that 41 states are in the potential range of aedes aegypti or ae ...	2016	27490117
one-pot fabrication of silver nanocrystals using nicandra physalodes: a novel route for mosquito vector control with moderate toxicity on non-target water bugs.	mosquitoes (diptera: culicidae) as vectors for important diseases and parasites causing millions of deaths every year. the use of synthetic pesticides against culicidae leads to resistance and environmental concerns. therefore, eco-friendly control tools are a priority. in this research, nicandra physalodes-mediated synthesis of silver nanoparticles (ag nps) was conducted, in order to control larval populations of three important mosquito vectors, anopheles stephensi, aedes aegypti and culex qui ...	2016	27473981
a detailed comparative analysis of codon usage bias in zika virus.	zika virus (zikv) is a member of the family flaviviridae and its genome consists of a single-stranded positive sense rna molecule with 10,794 nucleotides. clinical manifestations of disease caused by zikv infection range from asymptomatic cases to an influenza-like syndrome. there is an increasing concern about the possible relation among microcephaly and zikv infection. to get insight into the relation of codon usage among viruses and their hosts is extremely important to understand virus survi ...	2016	27449601
outbreak of zika virus infection, chiapas state, mexico, 2015, and first confirmed transmission by aedes aegypti mosquitoes in the americas.	after decades of obscurity, zika virus (zikv) has spread through the americas since 2015 accompanied by congenital microcephaly and guillain-barré syndrome. although these epidemics presumably involve transmission by aedes aegypti, no direct evidence of vector involvement has been reported, prompting speculation that other mosquitoes such as culex quinquefasciatus could be involved.	2016	27436433
egg and fourth instar larvae gut of aedes aegypti as a source of stem cells.	according to the world health organization, 2015 registered more than 1.206.172 cases of dengue in the americas. recently, the aedes aegypti has been not only related to dengue, but also with cases of zika virus and chikungunya. due to its epidemiological importance, this study characterized the morphology of the embryonated eggs of a. aegypti and provided a protocol to culture stem cells from eggs and digestive tract of fourth instar larvae in order to examine cell biology and expression of mar ...	2016	27401144
yellow fever remains a potential threat to public health.	yellow fever (yf) remains a serious public health threat in endemic countries. the recent re-emergence in africa, initiating in angola and spreading to democratic republic of congo and uganda, with imported cases in china and kenya is of concern. there is such a shortage of yf vaccine in the world that the world health organization has proposed the use of reduced doses (1/5) during emergencies. in this short communication, we discuss these and other problems including the risk of spread of yf to ...	2016	27400066
screening of blood donations for zika virus infection - puerto rico, april 3-june 11, 2016.	transfusion-transmitted infections have been documented for several arboviruses, including west nile and dengue viruses (1). zika virus, a flavivirus transmitted primarily by aedes aegypti mosquitoes that has been identified as a cause of congenital microcephaly and other serious brain defects (2), became recognized as a potential threat to blood safety after reports from a 2013-2014 outbreak in french polynesia. blood safety concerns were based on very high infection incidence in the population ...	2016	27337368
[zika virus and zika viral disease].	since zika virus (zikv) has firstly been isolated in 1947, uganda, outbreaks of zika fever have been reported in many areas such as in africa, southeast asia and america. imported cases in china also have been reported. zika virus belongs to the family flaviviridae, genus flavivirus, and include africa subtype and asia subtype. it is a mosquito-borne virus primarily transmitted by aedes aegypti mosquitoes. sexual transmission, blood transmission and mother-to-fetus transmission were also reporte ...	2016	27295893
dengue, chikungunya … and the missing entity - zika fever: a new emerging threat.	zika virus (zikv), a relative newcomer from the flavivirus group that includes dengue, japanese encepahalitis and yellow fever, is one of the emerging pathogens that is fast transcending geographical boundaries. it is a vector-borne disease transmitted by the same aedes aegypti and aedes albopictus, which cause dengue and chikungunya. in addition to the vector-mediated transmission of zika fever, probable human-to-human transmission through exchange of body fluids, including sexual and perinatal ...	2016	27257326
zika virus infection: current concerns and perspectives.	the zika virus outbreaks highlight the growing importance need for a reliable, specific and rapid diagnostic device to detect zika virus, as it is often recognized as a mild disease without being identified. many zika virus infection cases have been misdiagnosed or underreported because of the non-specific clinical presentation. the aim of this review was to provide a critical and comprehensive overview of the published peer-reviewed evidence related to clinical presentations, various diagnostic ...	2016	27236440
an infectious cdna clone of zika virus to study viral virulence, mosquito transmission, and antiviral inhibitors.	the asian lineage of zika virus (zikv) has recently caused epidemics and severe disease. unraveling the mechanisms causing increased viral transmissibility and disease severity requires experimental systems. we report an infectious cdna clone of zikv that was generated using a clinical isolate of the asian lineage. the cdna clone-derived rna is infectious in cells, generating recombinant zikv. the recombinant virus is virulent in established zikv mouse models, leading to neurological signs relev ...	2016	27198478
[infestation status aedes albopictus and related mosquito-borne infectious disease risk in central urban area in shanghai].	to evaluate aedes albopictus infestation status in the central urban area of shanghai, and analyze the related epidemic risk of mosquito-borne infectious disease.	2016	27188346
experimental studies of susceptibility of italian aedes albopictus to zika virus.	we report a study on vector competence of an italian population of aedes albopictus for zika virus (zikv). ae. albopictus was susceptible to zikv infection (infection rate: 10%), and the virus could disseminate and was secreted in the mosquito's saliva (dissemination rate: 29%; transmission rate: 29%) after an extrinsic incubation period of 11 days. the observed vector competence was lower than that of an ae. aegypti colony tested in parallel.	2016	27171034
zika virus - emergence, evolution, pathology, diagnosis, and control: current global scenario and future perspectives - a comprehensive review.	this review converses the zika virus which has attained global concern due to its rapid pandemic potential and impact on humans. though zika virus was first isolated in 1947, till the recent large-scale outbreak which occurred in micronesia, in 2007, the virus was placed into the innocuous pathogen category. the world health organization on 1 february 2016 declared it as a 'public health emergency of international concern.' of the note, american as well as pacific island strains/isolates is rela ...	2016	27158761
[zika virus infection or the future of infectious diseases].	zika virus belongs to the flaviridae, an extended phylogenetic family containing dengue or yellow fever, viruses whose shared main vector are aedes aegypti mosquitoes. the virus originally came from central african simian reservoirs and, from there, expanded rapidly across the pacific to south america. the disease is an example of exantematic fever usually mild. mortality is very low and mainly limited to secondary guillain-barré or fetal microcephaly cases. diagnostic confirmation requires a rt ...	2016	27156484
search and one will find: zika virus everywhere.		2016	27076507
one-step synthesis of polydispersed silver nanocrystals using malva sylvestris: an eco-friendly mosquito larvicide with negligible impact on non-target aquatic organisms.	the synthesis of eco-friendly nanoparticles is evergreen branch of nanoscience with a growing number of biomedical implications. in this study, we investigated the synthesis of polydisperse and stable silver nanoparticles (agnp) using a cheap leaf extract of malva sylvestris (malvaceae). bio-reduced agnp were characterized by uv-visible spectrophotometry, fourier transform infrared spectroscopy (ftir), x-ray diffraction analysis (xrd), atomic force microscopy (afm), scanning electron microscopy ...	2016	27075309
zika virus: implications for public health.	the world health organization has declared the current zika virus (zikv) epidemic a public health emergency of international concern. lack of vaccines and reliable diagnostic tests, broad geographical distribution of mosquito species that can transmit the virus, and absence of population immunity in newly affected countries are causes for concern. although most infected persons are asymptomatic, zikv has been associated with a rise in cases of neurological complications and fetal central nervous ...	2016	27048745
zika virus.	zika virus (zikv) is an arthropod-borne virus (arbovirus) in the genus flavivirus and the family flaviviridae. zikv was first isolated from a nonhuman primate in 1947 and from mosquitoes in 1948 in africa, and zikv infections in humans were sporadic for half a century before emerging in the pacific and the americas. zikv is usually transmitted by the bite of infected mosquitoes. the clinical presentation of zika fever is nonspecific and can be misdiagnosed as other infectious diseases, especiall ...	2016	27029595
zika virus: yet another emerging threat to nepal.	zika virus (zikv) is a flavivirus with single stranded rna related to yellow fever, dengue, west nile, and japanese encephalitis viruses and is transmitted by aedes mosquitoes primarily by aedes aegipti which is widely distributed in nepal. zikv was first identified incidentally in rhesus monkey in uganda in 1947 and human infection in 1952; and by now outbreaks of zikv disease have been recorded in africa, the americas, asia and the pacific. the world health organization (who) has recently decl ...	2016	27005721
larvicidal and pupicidal activities of alizarin isolated from roots of rubia cordifolia against culex quinquefasciatus say and aedes aegypti (l.) (diptera: culicidae).	the mosquitocidal activities of different fractions and a compound alizarin from the methanol extract of rubia cordifolia roots were evaluated on larvae and pupae of culex quinquefasciatus say and aedes aegypti (l.) (diptera: culicidae). larvae and pupae were exposed to concentrations of 2.5, 5.0, 7.5 and 10 ppm for fractions and 0.5, 1.0, 1.5 and 2.0 ppm for compound. after 24 h, the mortality was assessed and the lc50 and lc90 values were estimated for larvae and pupae. among the 23 fractions ...	2016	27004695
revision to cdc's zika travel notices: minimal likelihood for mosquito-borne zika virus transmission at elevations above 2,000 meters.	since may 2015, when zika virus, a flavivirus transmitted primarily by aedes aegypti mosquitoes, was reported in brazil, the virus has rapidly spread across the region of the americas and the caribbean. the association between maternal zika virus infection and adverse fetal and reproductive outcomes, including microcephaly, prompted cdc to issue a level 2 alert travel notice* for the 37 countries and u.s. territories (at the national and territorial level) that have reported recent zika virus tr ...	2016	26985965
the convergence of a virus, mosquitoes, and human travel in globalizing the zika epidemic.	the zika virus was first identified in 1947 in the zika forest of uganda. it was discovered in a rhesus monkey that had been placed in a cage on a sentinel platform in the forest by the virus research institute. when this writer visited the institute and the zika forest in 1961, work was underway to identify mosquito species at various levels of the tree canopy. this was done through the placement of traps at various levels of a 120-foot-high steel tower which this writer climbed. at that time, ...	2016	26969497
zika virus outbreak in brazil.	zika virus (zikv) infection is spreading rapidly within the americas after originating from an outbreak in brazil. we describe the current zikv infection epidemic in brazil and the neurological symptoms arising. first cases of an acute exanthematic disease were reported in brazil's northeast region at the end of 2014. in march 2015, autochthonous zikv was determined to be the causative agent of the exanthematic disease. as cases of neurological syndromes in regions where zikv, dengue and/or chik ...	2016	26927450
local transmission of zika virus--puerto rico, november 23, 2015-january 28, 2016.	zika virus, a mosquito-borne flavivirus, spread to the region of the americas (americas) in mid-2015, and appears to be related to congenital microcephaly and guillain-barré syndrome (1,2). on february 1, 2016, the world health organization (who) declared the occurrence of microcephaly cases in association with zika virus infection to be a public health emergency of international concern.* on december 31, 2015, puerto rico department of health (prdh) reported the first locally acquired (index) c ...	2016	26890470
interim guidelines for prevention of sexual transmission of zika virus - united states, 2016.	zika virus is a mosquito-borne flavivirus primarily transmitted by aedes aegypti mosquitoes (1,2). infection with zika virus is asymptomatic in an estimated 80% of cases (2,3), and when zika virus does cause illness, symptoms are generally mild and self-limited. recent evidence suggests a possible association between maternal zika virus infection and adverse fetal outcomes, such as congenital microcephaly (4,5), as well as a possible association with guillain-barré syndrome. currently, no vaccin ...	2016	26866485
comparative identification of protein profiles and major allergens of saliva, salivary gland and whole body extracts of mosquito species in thailand.	allergic reactions to mosquito bites, such as generalized urticaria or severe local reactions are common problems worldwide. the diverse sources of allergen prepared from different mosquito body parts usage are a major obstacle to obtaining safe and effective tests and immunotherapy for mosquito bite allergy. thus, the reactions are often not recognized and allergen immunotherapy is seldom used for severe reaction to mosquito bites. in a search for appropriate allergen sources, the protein profi ...	2016	21038786
[ultrastructural alterations in larvae of aedes aegypti subject to labdane diterpene isolated from copaifera reticulata (leguminosae) and a fraction enriched with tannins of magonia pubescens (sapindaceae)].	dengue is an important public health problem in many countries and its main vector aedes aegypti, is the mosquito most adapted to urban areas. for the first time, the mechanism of action of labdane diterpenoid extracted from copaifera reticulata and the fraction enriched of catechin tannins extracted from magonia pubescens is demonstrated through ultrastructural alterations of aedes aegypti larvae.	2016	21468474
[comprehensive surveillance system for dengue prevention].	a comprehensive ecosystem approach-based surveillance system was designed and implemented for dengue prevention in cotorro municipality at the city of havana. cuba, geographically situated near those countries with high dengue incidence, and having a preventive approach as a premise of its public healthcare system, has to adopt measures to prevent new dengue epidemics. the environmental, entomological and clinical/epidemiological elements of the surveillance system were integrated and combined w ...	2016	23427456
calibration and evaluation of field cage for oviposition study with aedes (stegomyia) aegypti female (l.) (diptera: culicidae).	differences among results gathered from insect behavior studies conducted in laboratory and field situations are due to ambient variables that differ greatly between both environments. in laboratory studies the environmental conditions can be controlled whereas in field temperature, humidity and air velocity vary uncontrollably. the objective of this study was to calibrate and evaluate an experimental area (field cage) (14 x 7 x 3.5 m) subdivided into eight test cages (2.5 x 2.5 x 2 m) for use i ...	2016	18813752
[entomological evaluation of the risk of urban outbreak of yellow fever in 2008 in abidjan, côte d'ivoire].	in april 2008, seven years after the outbreak of 2001, abidjan faced another epidemic of yellow fever. three weeks after the case detected in the commune of treichville at 28(th) of april 2008, two other cases were notified, the first one in port-bouet and the second one in cocody located respectively in the south and north of the city of abidjan. in order to determine a large-scale risk of urban yellow fever epidemic, epidemiological and entomological investigations were conducted by the minist ...	2016	23693032
an anti-mosquito mixture for domestic use, combining a fertiliser and a chemical or biological larvicide.	plant saucers are an important larval habitat for aedes aegypti and ae. albopictus in peridomestic situations. because npk fertilisers in plant containers tend to enhance the oviposition of these species, we investigated the effects of bacillus thuringiensis var. israelensis, spinosad, pyriproxyfen and diflubenzuron larvicides in combination with fertiliser on the adult emergence and fecundity of the mosquitoes coming from plant saucers in controlled greenhouse experiments. npk + larvicide (npk- ...	2016	26414239
importance of thr(328) and thr(369) for functional maintenance of two receptor-binding β-hairpins of the bacillus thuringiensis cry4ba toxin: implications for synergistic interactions with cyt2aa2.	bacillus thuringiensis cry4ba mosquito-active toxin was previously shown to utilize two critical loop-residues, tyr(332) and phe(364) which are respectively located in β2-β3 and β4-β5 loops, for synergistic interactions with its alternative receptor-cyt2aa2. here, structural analysis of the cry4ba-receptor-binding domain revealed that its n-terminal subdomain encompasses β2-β3 and β4-β5 hairpins which are stabilized by inter-hairpin hydrogen bonding between thr(328) in β2 and thr(369) in β5. fun ...	2016	26692482
alkaline phosphatases are involved in the response of aedes aegypti larvae to intoxication with bacillus thuringiensis subsp. israelensis cry toxins.	bacillus thuringiensis subsp. israelensis (bti) is a natural pathogen of dipterans widely used as a biological insecticide for mosquito control. to characterize the response of mosquitoes to intoxication with bti, the transcriptome profile of bti-exposed susceptible aedes aegypti larvae was analysed using illumina rna-seq. gene expression of 11 alkaline phosphatases (alps) was further investigated by real time quantitative polymerase chain reaction and alp activity was measured in the susceptibl ...	2016	26663676
toxin stability improvement and toxicity increase against dipteran and lepidopteran larvae of bacillus thuringiensis crystal protein cry2aa.	bacillus thuringiensis δ-endotoxins are the most widely used biopesticides for controlling economically important crop pests and disease vectors. improving their efficacy is of great benefit. here, an improvement in cry2aa δ-endotoxin toxicity was attempted via a cry gene over expression system using p20 from b. thuringiensis israelensis.	2016	26910489
local mosquito-borne transmission of zika virus - miami-dade and broward counties, florida, june-august 2016.	during the first 6 months of 2016, large outbreaks of zika virus disease caused by local mosquito-borne transmission occurred in puerto rico and other u.s. territories, but local mosquito-borne transmission was not identified in the continental united states (1,2). as of july 22, 2016, the florida department of health had identified 321 zika virus disease cases among florida residents and visitors, all occurring in either travelers from other countries or territories with ongoing zika virus tran ...	2016	27684886