Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
laboratory and semi-field evaluations of two (transfluthrin) spatial repellent devices against aedes aegypti (l.) (diptera: culicidae).two transfluthrin-based spatial repellent products (raid dual action insect repellent and home freshener and raid shield (currently not commercially available), sc johnson, racine wi) were evaluated for spatial repellent effects against female aedes aegypti (l.) mosquitoes under laboratory (wind tunnel) and semi-field (outdoor enclosure) conditions. the placement of either product in the wind tunnel significantly reduced host-seeking behaviors. the mean baseline (control) landing counts for the ...201728511270
reemergence of yellow fever in ethiopia after 50 years, 2013: epidemiological and entomological investigations.yellow fever (yf) is a viral hemorrhagic disease transmitted by aedes mosquito species. approximately, 200,000 cases and 30,000 deaths occur worldwide every year. in ethiopia, the last outbreak was reported in 1966 with 2200 cases and 450 deaths. a number of cases with deaths from unknown febrile illness reported from south ari district starting from november 2012. this investigation was conducted to identify the causative agent, source of the outbreak and recommend appropriate interventions.201728506254
aedes aegypti (l.) mosquitoes in trinidad, west indies: longevity case studies.few laboratory and field studies have reported long survival periods for ae. aegypti females and even fewer have designed experiments to characterize this important life history trait. this study was conducted under laboratory conditions to determine the number of blood meals taken by individual females, the number of eggs laid per individual female, the length of the gonotrophic cycle, and the duration of female survival. the results showed individual females oviposited between 670 and 1,500 eg ...201728504438
influence of shading and pedestrian traffic on the preference of aedes (stegomyia) aegypti (diptera: culicidae) for oviposition microenvironments.aedes aegypti mosquitoes are highly adaptable to abiotic stimuli. to evaluate the influence of shading and pedestrian traffic on the preference of ae. aegypti for oviposition microenvironments, 20 sites were sampled weekly using ovitraps within the perimeter of universidade federal do espírito santo, located in são mateus, espírito santo, brazil. a spatial and statistical analysis was performed in order to assess the relationship between shading time, pedestrian traffic, and the presence of biol ...201728504433
isolation of an insulin-like peptide from the asian malaria mosquito, anopheles stephensi, that acts as a steroidogenic gonadotropin across diverse mosquito taxa.many insulin-like peptides (ilps) have been identified in insects, yet only a few were isolated in their native form for structural and functional studies. antiserum produced to ilp3 in aedes aegypti was used in a radioimmunoassay to monitor the purification of an ilp from heads of adult an. stephensi and recognized the ilp in other immunoassays. the structure of the purified peptide matched that predicted for the ilp3 in this species. the native form stimulated ecdysteroid production by ovaries ...201728502740
metazoan ribosome inactivating protein encoding genes acquired by horizontal gene transfer.ribosome inactivating proteins (rips) are rna n-glycosidases that depurinate a specific adenine residue in the conserved sarcin/ricin loop of 28s rrna. these enzymes are widely distributed among plants and their presence has also been confirmed in several bacterial species. recently, we reported for the first time in silico evidence of rip encoding genes in metazoans, in two closely related species of insects: aedes aegypti and culex quinquefasciatus. here, we have experimentally confirmed the p ...201728500327
larval interference competition between the native neotropical mosquito limatus durhamii and the invasive aedes aegypti improves the fitness of both species.interspecific competition with native species during biological invasions can sometimes limit alien expansion. we aimed to determine the potential ecological effects of limatus durhamii theobald 1901, a native neotropical mosquito (diptera: culicidae) species, on the invasive species aedes (stegomyia) aegypti (linnaeus 1762) that breeds in the same artificial water containers. development time and adult dry mass were measured in 3 rearing conditions: control (a single larva), intraspecific compe ...201728497885
assessing the effect of selection with deltamethrin on biological parameters and detoxifying enzymes in aedes aegypti (l.).resistance to insecticides through one or several mechanisms has a cost for an insect in various parameters of its biological cycle. the present study evaluated the effect of deltamethrin on detoxifying enzymes and biological parameters in a population of aedes aegypti selected for 15 generations. the enzyme activities of alpha- and beta-esterases, mixed-function oxidases and glutathione-s-transferases were determined during selection, along with biological parameters.201728485040
what does heat tell a mosquito? characterization of the orientation behaviour of aedes aegypti towards heat sources.the use of heat as a cue for the orientation of haematophagous insects towards hot-blooded hosts has been acknowledged for many decades. in mosquitoes, thermoreception has been studied at the molecular, physiological and behavioural levels, and the response to heat has been evaluated in multimodal contexts. however, a direct characterization of how these insects evaluate thermal sources is still lacking. in this study we characterize aedes aegypti thermal orientation using a simple dual choice p ...201728479122
eco-friendly synthesis of silver nanoparticles and its larvicidal property against fourth instar larvae of aedes aegypti.in this study, larvicidal activity of silver nanoparticles (agnps) synthesised using apple extract against fourth instar larvae of aedes aegypti was determined. as a result, the agnps showed moderate larvicidal effects against ae. aegypti larvae (lc50 = 15.76 ppm and lc90 = 27.7 ppm). in addition, comparison of larvicidal activity performance of agnps at high concentration prepared using two different methods showed that ae. aegypti larvae was fully eliminated within the duration of 2.5 h. from ...201728476997
photo-induced and phytomediated synthesis of silver nanoparticles using derris trifoliata leaf extract and its larvicidal activity against aedes aegypti.due to the increased development of resistance of vectors against synthetic insecticides and chemical drugs, plant based insecticides serve as promising biocontrol agents for effective vector control. green approach for the synthesis of nanoparticles has been attained using environmentally safe, non-toxic plant extracts. the present study was aimed to investigate the potent larvicidal activity of silver nanoparticles (agnps) produced by derris trifoliata leaf extract in relation to the various c ...201728460330
allergens involved in the cross-reactivity of aedes aegypti with other arthropods.cross-reactivity between aedes aegypti and mites, cockroaches, and shrimp has been previously suggested, but the involved molecular components have not been fully described.201728434865
n-aryl-n'-ethyleneaminothioureas effectively inhibit acetylcholinesterase 1 from disease-transmitting mosquitoes.vector control of disease-transmitting mosquitoes by insecticides has a central role in reducing the number of parasitic- and viral infection cases. the currently used insecticides are efficient, but safety concerns and the development of insecticide-resistant mosquito strains warrant the search for alternative compound classes for vector control. here, we have designed and synthesized thiourea-based compounds as non-covalent inhibitors of acetylcholinesterase 1 (ache1) from the mosquitoes anoph ...201728433681
identification of the septate junction protein gliotactin in the mosquito aedes aegypti: evidence for a role in increased paracellular permeability in larvae.septate junctions (sjs) regulate paracellular permeability across invertebrate epithelia. however, little is known about the function of sj proteins in aquatic invertebrates. in this study, a role for the transmembrane sj protein gliotactin (gli) in the osmoregulatory strategies of larval mosquito (aedes aegypti) was examined. differences in gli transcript abundance were observed between the midgut, malpighian tubules, hindgut and anal papillae of a. aegypti, which are epithelia that participate ...201728432154
allatotropin: a pleiotropic neuropeptide that elicits mosquito immune responses.allatotropins (at) are neuropeptides with pleotropic functions on a variety of insect tissues. they affect processes such as juvenile hormone biosynthesis, cardiac rhythm, oviduct and hindgut contractions, nutrient absorption and circadian cycle. the present work provides experimental evidence that at elicits immune responses in two important mosquito disease vectors, anopheles albimanus and aedes aegypti. hemocytes and an immune-competent mosquito cell line responded to at by showing strong mor ...201728426765
efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae).the current zika health crisis in the americas has created an intense interest in mosquito control methods and products. mosquito vectors of zika are of the genus aedes, mainly the yellow fever mosquito, aedes aegypti. l. the use of repellents to alter mosquito host seeking behavior is an effective method for the prevention of mosquito-borne diseases. a large number of different spray-on repellents and wearable repellent devices are commercially available. the efficacies of many repellents are u ...201728423421
habitat and density of oviposition opportunity influences aedes aegypti (diptera: culicidae) flight distance.understanding the dispersal of aedes (aegypti (l.) diptera: culicidae) after consuming a potentially infectious bloodmeal is an important part of controlling the spread of the arboviruses it transmits. because of the impact on abundance, removal of oviposition sites is a key component of vector control. however, source reduction around a case may encourage dispersal of potentially infected vectors. we compare the effect of oviposition site availability on ae. aegypti dispersal behavior within 30 ...201728419273
the impact of temperature and body size on fundamental flight tone variation in the mosquito vector aedes aegypti (diptera: culicidae): implications for acoustic lures.aedes aegypti (l.) males use female flight tone as a means of mate localization. by playing the sound of a flying female, males can be attracted to a trap to monitor mosquito populations and the progress of transgenic male releases. however, the female flight tone used to attract males needs to be optimized to maximize trap effectiveness. the fundamental frequency of female flight tone could be influenced by both body size and ambient temperature. however, no analysis yet has considered both the ...201728402550
immunohistochemical mapping and transcript expression of the gpa2/gpb5 receptor in tissues of the adult mosquito, aedes aegypti.gpa2/gpb5 is a glycoprotein hormone found in most bilateral metazoans including the mosquito, aedes aegypti. to elucidate physiological roles and functions of gpa2/gpb5, we aim to identify prospective target tissues by examining the tissue- and sex-specific expression profile of its receptor, the leucine-rich repeat-containing g protein-coupled receptor 1 (lgr1) in the adult mosquito. western analyses using a heterologous system with cho-k1 cells, transiently expressing a. aegypti lgr1, yielded ...201728401307
full-genome dengue virus sequencing in mosquito saliva shows lack of convergent positive selection during transmission byaedes aegypti.like other pathogens with high mutation and replication rates, within-host dengue virus (denv) populations evolve during infection of their main mosquito vector,aedes aegypti. within-host denv evolution during transmission provides opportunities for adaptation and emergence of novel virus variants. recent studies of denv genetic diversity failed to detect convergent evolution of adaptive mutations in mosquito tissues such as midgut and salivary glands, suggesting that convergent positive selecti ...201729497564
[zika: probability of establishment of its vector, aedes aegypti, in chile].the zika virus has raised world alarm in recent years, representing a major public health problem. in this study we evaluated the potential risk of exposure to zika virus in chile, associated with the probability of establishment of the vector aedes aegypti in the country. niche modelling techniques were used to project the bioclimatic requirements of the vector (global niches), identifying zones of high suitability for the species within the country. then, the potential distribution of the vect ...201729488548
[(assessment of risk for arbovirus infections in russia)].the sharply increased incidence and geographical expansion of arbovirus diseases in recent years suggest that some russian areas are vulnerable to vector-borne diseases and that it is important to elaborate control programs to ensure bio- logical safety in our country. moreover, the main vectors of the pathogens of arbovirus infections (zika fever, dengue fever, chikungunya fevers), such as aedes aegypti and ae.albopictus, have been registered on the black sea coast of the caucasus. the programs ...201730721608
larvicidal and pupicidal activities of crude and fractionated extracts of acacia pennata (l.) willd. subsp insuavis shoot tips against aedes aegypti (l.) (diptera: culicidae).acacia pennata subsp insuavis, or cha-om in thai, is a common vegetablefound in thailand. it has been used as a medicinal herb for a long time. from theliterature, antinociceptive, anti-inflammatory, antimicrobial, and anti-helminthicactivities were reported. in this study, we investigated two new actions of thisplant: larvicide and pupicide. the crude ethanolic and fractionated extracts of a.pennata shoot tips were tested against aquatic stages of the dengue virus vector,aedes aegypti mosquito. ...201729644817
[clinical and epidemiological profile of chikungunya infection in hospital patients in 2015 in mérida, mexicoperfil clínico-epidemiológico da infecção por chikungunya em casos hospitalares atendidos em 2015 em mérida, méxico].describe the epidemiological profile and analyze clinical manifestations by age group in chikungunya patients treated in 2015 in a mérida, mexico general hospital.201731391823
erratum: efficacy of some wearable devices compared with spray-on insect repellents for the yellow fever mosquito, aedes aegypti (l.) (diptera: culicidae). 201728365772
tropical viruses approaching europe.during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ...201729952164
tropical viruses approaching europe.during the last years, headlines about the advance of tropical infectious diseases, which are transmitted by mosquitos, become more frequent. some of these mosquitos are classified as invasive species in europe. the world health organization (who) and the european center for disease prevention and control (ecdc) are enhancing the observation of the geographical spread of these species. especially the asian tiger mosquito and the yellow fever mosquito are in focus. in germany the vector competenc ...201729952163
ultrasound is not useful as a screening tool for dengue fever.dengue fever is a tropical disease that is transmitted by female aedes aegypti mosquitos. early diagnosis is necessary to reduce the mortality and morbidity associated with the disease. a combination of clinical, laboratory, and sonography findings can be potentially helpful in making an early diagnosis of dengue fever. there is paucity of literature on the use of ultrasound for dengue fever screening; hence, the primary objective of the study was to evaluate the utility of ultrasound as a scree ...201729657635
fly ash-based water dispersible powder formulation of bacillus thuringiensis var. israelensis: development & laboratory evaluation against mosquito immatures.bacillus thuringiensis var. israelensis (bti) formulations are presently being used for insect control. in this study, a water dispersible powder (wdp) formulation using fly ash (fa) as a carrier material was developed and studied for its activity against the larval stages of major mosquito vector species.201729664029
infectivity of chordodes nobilii larvae (gordiida: nematomorpha).the gordiids are freshwater representatives of the parasite phylum nematomorpha that function asa link between aquatic and terrestrial ecosystems. in recent years, different ecotoxicologic studies have beenmade with the south-american gordiid species, chordodes nobilii, that have demonstrated the capacity of thisgroup to act as a bioindicator of contamination. despite the gordiida’s ecologic relevance, further studies arestill needed to elucidate different aspects of the biology of the class, an ...201729465953
environmental factors can influence dengue reported cases.global climate changes directly affect the natural environment and contribute to an increase in the transmission of diseases by vectors. among these diseases, dengue is at the top of the list. the aim of our study was to understand the consequences of temporal variability of air temperature in the occurrence of dengue in an area comprising seven municipalities of the greater são paulo.201729451659
evaluation of phytosynthesised silver nanoparticles from leaf extracts of leucas aspera and hyptis suaveolens and their larvicidal activity against malaria, dengue and filariasis vectors.the present study deals with the green synthesis of silver nanoparticle from the aqueous leaf extracts of leucas aspera and hyptis suaveolens as reducing agent and to investigate the larvicidal activity of synthesized silver nanoparticles. the synthesized silver nanoparticles were characterized by ultraviolet and visible absorption spectroscopy (uv), fourier transform-infrared spectroscopy (ft-ir), x-ray spectroscopy (xrd), field emission scanning electron microscope (fesem) and high-resonance t ...201729774292
biological control of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps.mosquitoes can act as vectors of important diseases such as malaria, dengue, zika virus, yellow fever, chikungunya and mayaro fever, in addition to filariasis. the use of insecticides, larvicides, bed nets and repellents, besides the use of drugs as chemoprevention and the treatment of the sick are currently the pillars of the control of these vectors. we studied the biological control against of anopheles darlingi, aedes aegypti and culex quinquefasciatus larvae using shrimps of the species m. ...201729774286
estimating the size of aedes aegypti populations from dengue incidence data: implications for the risk of yellow fever outbreaks.in this paper we present a model to estimate the density of aedes mosquitoes in a community affected by dengue. the method consists in fitting a continuous function to the incidence of dengue infections, from which the density of infected mosquitoes is derived straightforwardly. further derivations allow the calculation of the latent and susceptible mosquitoes' densities, the sum of the three equals the total mosquitoes' density. the method is illustrated with the case of the risk of urban yello ...201730137722
transcription profiling for defensins of aedes aegypti (diptera: culicidae) during development and in response to infection with chikungunya and zika viruses.aedes aegypti (l.) is a vector of chikungunya, dengue, yellow fever and zika viruses. these viruses encounter a variety of induced defense responses from the innate immune system of the mosquito. we cloned defensin a from ae. aegypti using laboratory populations originating from key west and orlando, florida. to characterize inducible immune defensin peptides, we examined the defensin a (defa) and defensin c (defc) expression through time course studies using quantitative real-time pcr. we obser ...201828968775
mapping the spatial distribution of aedes aegypti and aedes albopictus.mosquito-borne infectious diseases, such as rift valley fever, dengue, chikungunya and zika, have caused mass human death with the transnational expansion fueled by economic globalization. simulating the distribution of the disease vectors is of great importance in formulating public health planning and disease control strategies. in the present study, we simulated the global distribution of aedes aegypti and aedes albopictus at a 5×5km spatial resolution with high-dimensional multidisciplinary ...201829191515
green synthesis of silver nanoparticles using holarrhena antidysenterica (l.) wall.bark extract and their larvicidal activity against dengue and filariasis vectors.the present study was carried out to evaluate the larvicidal potential of methanol, hexane, acetone, chloroform, and aqueous bark extracts of holarrhena antidysenterica (l.) wall. and silver nanoparticles (agnps) synthesized using aqueous bark extract against the third instar larvae of aedes aegypti l. and culex quinquefasciatus say. agnps were prepared by adding 10 ml of aqueous bark extract in 90 ml of 1 mm silver nitrate (agno3) solution. after 5 min of mixing, a change in color from yellow t ...201829250727
at the origin of a worldwide invasion: unraveling the genetic makeup of the caribbean bridgehead populations of the dengue vector aedes aegypti.human-driven global environmental changes have considerably increased the risk of biological invasions, especially the spread of human parasites and their vectors. among exotic species that have major impacts on public health, the dengue fever mosquito aedes aegypti originating from africa has spread worldwide during the last three centuries. although considerable progress has been recently made in understanding the history of this invasion, the respective roles of human and abiotic factors in s ...201829267872
field- and clinically derived estimates of wolbachia-mediated blocking of dengue virus transmission potential in aedes aegypti mosquitoes.the wmel strain of wolbachia can reduce the permissiveness of aedes aegypti mosquitoes to disseminated arboviral infections. here, we report that wmel-infected ae. aegypti (ho chi minh city background), when directly blood-fed on 141 viremic dengue patients, have lower dengue virus (denv) transmission potential and have a longer extrinsic incubation period than their wild-type counterparts. the wmel-infected mosquitoes that are field-reared have even greater relative resistance to denv infection ...201829279375
assessment of a trap based aedes aegypti surveillance program using mathematical modeling.the goal of this study was to assess the goodness-of-fit of theoretical models of population dynamics of aedes aegypti to trap data collected by a long term entomological surveillance program. the carrying capacity k of this vector was estimated at city and neighborhood level. adult mosquito abundance was measured via adults collected weekly by a network of sticky traps (mosquitraps) from january 2008 to december 2011 in vitória, espírito santo, brazil. k was the only free parameter estimated by ...201829304070
hypoxia-induced transcription factor signaling is essential for larval growth of the mosquito aedes aegypti.gut microbes positively affect the physiology of many animals, but the molecular mechanisms underlying these benefits remain poorly understood. we recently reported that bacteria-induced gut hypoxia functions as a signal for growth and molting of the mosquito aedes aegypti in this study, we tested the hypothesis that transduction of a gut hypoxia signal requires hypoxia-induced transcription factors (hifs). expression studies showed that hif-α was stabilized in larvae containing bacteria that in ...201829298915
group b wolbachia strain-dependent inhibition of arboviruses.mosquito-borne viruses, including zika virus (zikv) and dengue virus (denv), are global threats that continue to infect millions annually. historically, efforts to combat the spread of these diseases have sought to eradicate the mosquito population. this has had limited success. recent efforts to combat the spread of these diseases have targeted the mosquito population and the mosquito's ability to transmit viruses by altering the mosquito's microbiome. the introduction of particular strains of ...201829297702
zika virus infection modulates the bacterial diversity associated with aedes aegypti as revealed by metagenomic analysis.zika is a re-emerging infection that has been considered a major threat to global public health. currently at least 100 countries are at risk of zika virus (zikv) transmission. aedes aegypti is the main mosquito vector in the americas. this vector is exposed to, and interacts symbiotically with a variety of microorganisms in its environment, which may result in the formation of a lifetime association. here, the unknown effect that zikv exerts on the dynamic bacterial community harbored by this m ...201829293631
insecticide treated curtains and residual insecticide treatment to control aedes aegypti: an acceptability study in santiago de cuba.within the context of a field trial conducted by the cuban vector control program (aacp), we assessed acceptability of insecticide-treated curtains (itcs) and residual insecticide treatment (rit) with deltamethrin by the community. we also assessed the potential influence of interviewees' risk perceptions for getting dengue and disease severity.201829293501
n-substituted methyl maleamates as larvicidal compounds against aedes aegypti (diptera: culicidae).severe human arboviral diseases can be transmitted by the mosquito aedes aegypti (l.), including dengue, chikungunya, zika, and yellow fever. the use of larvicides in containers that can result as potential breeding places and cannot be eliminated is the main alternative in control programs. however, their continuous and widespread use caused an increase in insecticide-resistant populations of this mosquito. the aim of this study was to evaluate the effect of three n-substituted methyl maleamate ...201829290037
[dengue, zika, chikungunya and the development of vaccines].dengue (denv), zika (zikv) and chikungunya (chikv), three arbovirosis transmitted by aedes mosquitoes, have spread in recent decades in humid tropical and subtropical zones. dengue is epidemic in subtropical areas of argentina. denv infection confers lasting immunity against the infecting serotype but increases the risk of serious disease upon reinfection by any of the other three. the recombinant tetravalent vaccine dengvaxia® prevents severe dengue and hospitalization in seropositive subjects. ...201829360072
cytoplasmic glutamine synthetase gene expression regulates larval development in bactrocera dorsalis (hendel).in insects, glutamine synthetase (gs), a key enzyme in the synthesis of glutamine, has been reported to be associated with embryonic development, heat shock response, and fecundity regulation. however, little is known about the influence of gs on postembryonic development. in this study, we demonstrate that blocking the activity of gs in the oriental fruit fly (bactrocera dorsalis) with use of a gs-specific inhibitor (l-methionine s-sulfoximine), led to a significant delay in larval development, ...201829359358
the wolbachia strain wau provides highly efficient virus transmission blocking in aedes aegypti.introduced transinfections of the inherited bacteria wolbachia can inhibit transmission of viruses by aedes mosquitoes, and in ae. aegypti are now being deployed for dengue control in a number of countries. only three wolbachia strains from the large number that exist in nature have to date been introduced and characterized in this species. here novel ae. aegypti transinfections were generated using the walba and wau strains. in its native ae. albopictus, walba is maintained at lower density tha ...201829370307
fine-scale landscape genomics helps explain the slow spatial spread of wolbachia through the aedes aegypti population in cairns, australia.the endosymbiotic bacterium wolbachia suppresses the capacity for arbovirus transmission in the mosquito aedes aegypti, and can spread spatially through wild mosquito populations following local introductions. recent introductions in cairns, australia have demonstrated slower than expected spatial spread. potential reasons for this include: (i) barriers to ae. aegypti dispersal; (ii) higher incidence of long-range dispersal; and (iii) intergenerational loss of wolbachia. we investigated these th ...201829358725
current concerns and perspectives on zika virus co-infection with arboviruses and hiv.dissemination of vector-borne viruses, such as zika virus (zikv), in tropical and sub-tropical regions has a complicated impact on the immunopathogenesis of other endemic viruses such as dengue virus (denv), chikungunya virus (chikv) and human immunodeficiency virus (hiv). the consequences of the possible co-infections with these viruses have specifically shown significant impact on the treatment and vaccination strategies. zikv is a mosquito-borne flavivirus from african and asian lineages that ...201829352633
aerial ulv control of aedes aegypti with naled (dibrom) inside simulated rural village and urban cryptic habitats.we conducted aerial fixed wing ultra low volume (ulv) spray trials with naled to investigate penetration of exposed and simulated cryptic habitat within opened buildings, partially sealed buildings, and outdoor locations targeting sentinel adult aedes aegypti mosquitoes in north central florida. mortality was observed in open and closed buildings and outdoors, even in mosquitoes placed in cryptic habitats. observations on the impact of building type, mosquito exposure method such as placement in ...201829352307
reduction in musca domestica fecundity by dsrna-mediated gene knockdown.house flies (musca domestica) are worldwide agricultural pests with estimated control costs at $375 million annually in the u.s. non-target effects and widespread resistance challenge the efficacy of traditional chemical control. double stranded rna (dsrna) has been suggested as a biopesticide for m. domestica but a phenotypic response due to the induction of the rnai pathway has not been demonstrated in adults. in this study female house flies were injected with dsrna targeting actin-5c or ribo ...201829342168
zika virus in salivary glands of five different species of wild-caught mosquitoes from mexico.zika virus (zikv) is a mosquito-borne pathogen, and aedes aegypti has been identified as the main vector of the disease. other mosquito species in the aedes and culex genera have been suggested to have the potential for being competent vectors based on experimental exposition of mosquitoes to an infectious blood meal containing zikv. here, we report the isolation in cell culture of zikv obtained from different body parts of wild-caught female mosquitoes (ae. aegypti, ae. vexans, cx. quinquefasci ...201829339746
wolbachia-induced transcription factor gata4 suppresses ovary-specific genes blastoderm-specific protein 25d and imaginal disc growth factor.the endosymbiotic bacterium wolbachia infects a wide array of insect hosts and has been implicated in a range of biological modifications as a consequence of its infection. previously, it was shown that the transcription factor gata4 was significantly induced in wolbachia wmelpop-cla strain infected aedes aegypti whole mosquitoes and cells. here, we provide evidence that this induction also occurs in mosquito ovaries where the ovary-specific genes blastoderm-specific protein 25d (bsg25d) and ima ...201829336504
vibrational properties of bulk boric acid 2a and 3t polymorphs and their two-dimensional layers: measurements and density functional theory calculations.boric acid (h3bo3) is being used effectively nowadays in traps/baits for the management of aedes aegypti l. and aedes albopictus skuse species of mosquitoes, which are the main spreading vectors worldwide for diseases such as malaria, dengue, and zika. previously, we published results on the structural, electronic, and optical properties of its molecular triclinic h3bo3-2a and trigonal h3bo3-3t polymorphs within the framework of density functional theory (dft). because of the renewed importance ...201829328646
the discovery of arthropod-specific viruses in hematophagous arthropods: an open door to understanding the mechanisms of arbovirus and arthropod evolution?the discovery of an odd virus from hematophagous arthropods 40 years ago by stollar and thomas described cell fusing agent virus in cells derived from aedes aegypti mosquitoes. then came the report of kamiti river virus from ae. macintoshi in 1999, followed by worldwide reports of the discovery of other viruses of mosquitoes, ticks, and midges that replicate only in arthropods and not in vertebrates or in vertebrate cells. these viruses (now totaling at least 64 published) have genomes analogous ...201829324047
dengue hemorrhagic fever virus in saudi arabia: a review.dengue fever is a global disease with a spectrum of clinical manifestation ranging from mild febrile disease to a severe disease in the form of dengue hemorrhagic fever and dengue shock syndrome. dengue virus is one viral hemorrhagic fever that exists in the kingdom of saudi arabia in addition to alkhurma (alkhurma) hemorrhagic fever, chikungunya virus, crimean-congo hemorrhagic fever, and rift valley fever. the disease is limited to the western and south-western regions of saudi arabia, where a ...201829319426
qsar analysis of plant-derived compounds with larvicidal activity against zika aedes aegypti (diptera: culicidae) vector using freely available descriptors.we develop a qsar model for predicting the larvicidal activity of 60 plant-derived molecules against aedes aegypti l. (diptera: culicidae), vector of several diseases such as, dengue, yellow fever, chikungunya and zika virus. the balanced subsets method (bsm) based on k-means cluster analysis (k-mca) is employed to split the data set. the replacement method (rm) variable subset selection technique coupled with multivariable linear regression (mlr) proves to be successful for exploring 18,326 mol ...201829314584
krüppel homologue 1 acts as a repressor and an activator in the transcriptional response to juvenile hormone in adult mosquitoes.krüppel homologue 1 (kr-h1) is a zinc finger transcription factor that is upregulated in insects by juvenile hormone (jh) in metamorphosis and adult reproduction. the molecular function of kr-h1 in reproduction remains largely unknown. here we report that aakr-h1 functions as an important transcription regulator in adult female aedes aegypti mosquitoes. the amount of aakr-h1 protein increases with rising jh levels after adult emergence, reaches its peak at 48 h after eclosion, then decreases gra ...201829314423
aristololactam derivatives from the fruits of aristolochia contorta bunge.three new aristololactam derivatives, aristololactam w-y (1-3), and three known compounds (4-6) were isolated from the fruits of aristolochia contorta bunge. compounds 1 and 2 represent the first example of an n-ch2och3 aristololactam derivative from natural products. their structures were elucidated by 1d/2d nmr and hresims spectra. all of the isolated compounds were evaluated for their insecticidal activity against 4th instar larvae of aedes aegypti. compound 4 displayed insecticidal activity ...201829313366
the genomes of four meyerozymacaribbica isolates and novel insights into the meyerozymaguilliermondii species complex.yeasts of the meyerozyma guilliermondii species complex are widespread in nature and can be isolated from a variety of sources, from the environment to arthropods to hospital patients. to date, the species complex comprises the thoroughly studied and versatile m. guilliermondii, and the hard to distinguish m. caribbica, and candida carpophila here we report the whole-genome sequencing and de novo assembly of four m. caribbica isolates, identified with the most recent molecular techniques, derive ...201829311113
critical low temperature for the survival of aedes aegypti in taiwan.taiwan is geographically located in a region that spans both tropical and subtropical climates (22-25°n and 120-122°e). the taiwan centers for disease control have found that the ecological habitat of aedes aegypti appears only south of 23.5°n. low temperatures may contribute to this particular habitat distribution of ae. aegypti under the influence of the east asian winter monsoon. however, the threshold condition related to critically low temperatures remains unclear because of the lack of lar ...201829310716
environmentally benign nanometric neem-laced urea emulsion for controlling mosquito population in environment.the increasing risk of vector-borne diseases and the environmental pollution in the day-to-day life due to the usage of the conventional pesticides makes the role of nanotechnology to come into the action. the current study deals with one of the applications of nanotechnology through the formulation of neem urea nanoemulsion (nune). nune was formulated using neem oil, tween 20, and urea using the microfluidization method. prior to the development of nanoemulsion, the ratio of oil/surfactant/urea ...201829116538
functional characterization of the dual allatostatin-a receptors in mosquitoes.the neuropeptide allatostatin-a (asta) and its cognate receptors (astars) are involved in the modulation of feeding behavior, which in hematophagous insects includes the regulation of the disease vector-related behaviors, host seeking and blood feeding. in mosquitoes and other dipterans, there are two copies of astar, contrasting with the single copy found in other insects. in this study, we identified and cloned the dual astar system of two important disease vectors aedes aegypti and culex quin ...201829103918
genome sequence of a novel strain of a phasi charoen-like virus identified in zhanjiang, china.here, we report the genome sequence of a novel strain of a phasi charoen-like virus, isolated from aedes aegypti mosquitoes caught in zhanjiang province of china. phylogenetic analysis suggested that this virus belongs to a new genus, goukovirus, in the family bunyaviridae this is the first reported genome sequence of a phasi charoen-like virus identified in china.201829326198
behavior of detoxifying enzymes of aedes aegypti exposed to girgensohnine alkaloid analog and cymbopogon flexuosus essential oil.because mosquito control depend on the use of commercial insecticides and resistance has been described in some of them, there is a need to explore new molecules no resistant. in vivo effects of girgensohnine analog 2-(3,4-dimethoxyphenyl)-2-(piperidin-1-yl)acetonitrile dppa and cymbopogon flexuosus essential oil cfeo, on the detoxifying enzymes acetylcholinesterase (ache), glutathione-s-transferase (gst), nonspecific esterases (α- and β-), mixed function oxidases (mfo) and p-npa esterases were ...201829129589
multifaceted functional implications of an endogenously expressed trna fragment in the vector mosquito aedes aegypti.the mosquito aedes aegypti is the primary vector of human arboviral diseases caused by dengue, chikungunya and zika viruses. many studies have shown the potential roles of small rna molecules such as microrna, small interfering rna and piwi-interacting rna in vector mosquitoes. the function of trna fragments (trf), the newly discovered class of small rnas, in mosquitoes is not known. in this study, we show that specific trfs are expressed in significantly differential manner between males and fe ...201829364883
mosquito vector-associated microbiota: metabarcoding bacteria and eukaryotic symbionts across habitat types in thailand endemic for dengue and other arthropod-borne diseases.vector-borne diseases are a major health burden, yet factors affecting their spread are only partially understood. for example, microbial symbionts can impact mosquito reproduction, survival, and vectorial capacity, and hence affect disease transmission. nonetheless, current knowledge of mosquito-associated microbial communities is limited. to characterize the bacterial and eukaryotic microbial communities of multiple vector species collected from different habitat types in disease endemic areas ...201829375803
the effect of larval diet on adult survival, swarming activity and copulation success in male aedes aegypti (diptera: culicidae).control of aedes aegypti (l.) (diptera: culicidae) populations is vital for reducing the transmission of several pervasive human diseases. the success of new vector control technologies will be influenced by the fitness of laboratory-reared transgenic males. however, there has been relatively little published data on how rearing practices influence male fitness in aedes mosquitoes. in the laboratory, the effect of larval food availability on adult male fitness was tested, using a range of differ ...201829029298
synthesis and structure-activity relationships of carbohydrazides and 1,3,4-oxadiazole derivatives bearing an imidazolidine moiety against the yellow fever and dengue vector, aedes aegypti.1,3,4-oxadiazole and imidazolidine rings are important heterocyclic compounds exhibiting a variety of biological activities. in this study, novel compounds with oxadiazole and imidazolidine rings were synthesized from 3-(methylsulfonyl)-2-oxoimidazolidine-1-carbonyl chloride and screened for insecticidal activities. the proposed structures of the 17 synthesized compounds were confirmed using elemental analysis, infrared (ir), proton nuclear magnetic resonance (1 h-nmr), and mass spectroscopy.201828869331
screening of plant extracts and fractions on aedes aegypti larvae found in the state of mato grosso do sul (linnaeus, 1762) (culicidae).the constant use of chemical insecticides for aedes aegypti control has caused resistance in the mosquito populations. thus, the objective of this study was to analyze the larvicidal potential of extracts and fractions of plants on a. aegypti larvae. the analysis included sixty one extracts and twenty five fractions of fifty botanical species at concentrations of 0.25; 0.12; 0.06 to 0.03 mg ml-1; 4 replications and one negative control of dechlorinate water and 1% dmso; and a positive control wi ...201828640344
quantification through tlc-densitometric analysis, repellency and anticholinesterase activity of the homemade extract of indian cloves.the rise of the mosquitoes-transmitted diseases, like dengue, zika and chikungunya in brazil in the last years has increased concerns on protection against mosquitoes bites. however, the prohibitive prices of the commercially available repellents for the majority of the brazilian population has provoked a search for cheaper solutions, like the use of the homemade ethanolic extract of indian clove (syzygium aromaticum l.) as repellent, which has been reported as quite efficient by the local press ...201828913959
positional stable isotope tracer analysis reveals carbon routes during ammonia metabolism of aedes aegypti mosquitoes.in aedes aegypti females, the ammonia released during blood meal digestion is partially metabolized to facilitate the disposal of excess nitrogen. in this study, we used low- and high-resolution liquid chromatography-mass spectrometry (lc/ms) techniques to investigate the role of glucose during ammonia detoxification. mosquitoes were fed a blood meal supplemented with [1,2-13c2]glucose, and downstream metabolites were measured for 24 h. quantification of [13c] amino acids in the entire mosquito ...201828970248
zika and chikungunya virus detection in naturally infected aedes aegypti in ecuador.the wide and rapid spread of chikungunya (chikv) and zika (zikv) viruses represent a global public health problem, especially for tropical and subtropical environments. the early detection of chikv and zikv in mosquitoes may help to understand the dynamics of the diseases in high-risk areas, and to design data based epidemiological surveillance to activate the preparedness and response of the public health system and vector control programs. this study was done to detect zikv and chikv viruses i ...201828982578
molecular detection and characterization of wolbachia pipientis from culex quinquefasciatus collected from lahore, pakistan.the gram-negative, pleomorphic endosymbiont wolbachia is known to infect a large number of insects and other arthropods naturally. this bacterium modifies the host biology, mainly causing reproductive alterations including feminization, death of male, parthenogenesis, and importantly cytoplasmic incompatibility. wolbachia-induced cytoplasmic incompatibility results in nonviable offspring and vector population suppression. in addition, this bacterium rapidly spreads and propagates within the host ...201829141766
potential role for flubendazole in limiting filariasis transmission: observations of microfilarial sensitivity.flubendazole (flbz) is a potent and efficacious macrofilaricide after parenteral administration. studies in animal models and one trial in patients infected with onchocerca volvulus revealed that flbz elicits minimal effects on microfilariae (mf). severe complications after ivermectin (ivm) treatment of patients with high loa loa microfilaraemia are of great concern. we examined the potential of flbz to rapidly kill l. loa mf, the phenomenon proposed to underlie the complications. mf of l. loa w ...201829141764
complex wolbachia infection dynamics in mosquitoes with imperfect maternal transmission.dengue, malaria, and zika are dangerous diseases primarily transmitted by aedes aegypti, aedes albopictus, and anopheles stephensi. in the last few years, a new disease control method, besides pesticide spraying to kill mosquitoes, has been developed by releasing mosquitoes carrying bacterium wolbachia into the natural areas to infect the wild population of mosquitoes and block disease transmission. the bacterium is transmitted by infected mothers and the maternal transmission was assumed to be ...201829161848
bacterial exopolysaccharide (eps)-coated zno nanoparticles showed high antibiofilm activity and larvicidal toxicity against malaria and zika virus vectors.in this study, a novel and effective approach was performed to synthesize zno nanoparticles (zno nps) using the exopolysaccharides (eps) from the probiotic strain bacillus licheniformis dahb1. eps acted as reducing and stabilizing agent for the formation of eps-zno nps by co-precipitation method. structural characterization was investigated by a surface plasma resonance centered at 375nm in uv-vis spectrum. ftir spectrum exhibited functional groups with strong absorption peak at 3814.7-420cm-1. ...201829173489
facile green synthesis of zinc oxide nanoparticles using ulva lactuca seaweed extract and evaluation of their photocatalytic, antibiofilm and insecticidal activity.the bioactivity of semiconductor nanocomplexes has been poorly studied in the field of pesticide science. in this research, the synthesis of zinc nanoparticles was accomplished through new effortless green chemistry process, using the ulva lactuca seaweed extract as a reducing and capping agent. the production of u. lactuca-fabricated zno nanoparticles (ul-zno nps) was characterized by powder x-ray diffraction (xrd), uv-visible, fourier transform infrared (ftir) spectroscopy, selected area elect ...201829169140
first evidence of zika virus venereal transmission in aedes aegypti mosquitoes.aedes aegypti is considered the main zika virus (zikv) vector, and is thought to be responsible for the 2015-2016 outbreak in brazil. zika positive ae. aegypti males collected in the field suggest that vertical and/or venereal transmission of zikv may occur.201829185595
point-of-use nanobiosensor for detection of dengue virus ns1 antigen in adult aedes aegypti: a potential tool for improved dengue surveillance.dengue virus (denv) and its primary mosquito vectors aedes spp. have spread to every continent except antarctica, causing outbreaks and autochthonous transmission in previously disease-free regions. recently, the spread of other arboviruses carried by invasive aedes spp., such as chikungunya and zika, seem to be following similar trends as denv and have renewed interest in monitoring and modeling arboviruses within mosquito vectors. unfortunately, current commercially available detection methods ...201829182305
nanotitania crystals induced efficient photocatalytic color degradation, antimicrobial and larvicidal activity.textile industries release tonnes of harmful toxic dyes into the environment, causing severe effects on living organisms, including humans. mosquitoes vectors spread important diseases which cause millions of human deaths worldwide. to control mosquitoes a number of synthetic mosquitocidal agents have been employed but all these pesticides pose harmful effects to human health and non-target species and also led to resistance development in treated vectors. microbial strains are also developing r ...201829241121
diurnal temperature range and chikungunya virus infection in invasive mosquito vectors.climate strongly influences the geographic distribution and timing of mosquito-borne disease outbreaks. environmental temperature affects phenotypic traits of mosquitoes including vector competence for arboviruses mediated by changes in infection, extrinsic incubation period and in rates of transmission. most experiments, however, are done at constant temperatures. in nature, mosquitoes are more likely to experience daily fluctuations in temperature. here we compare disseminated infection (leg i ...201829040730
the effect of insecticidal stress on reproductive output of susceptible and field strains of aedes aegypti (diptera: culicidae).the phenomenon of high egg reproduction when mortality risk rises is common in mosquitoes. however, the phenomenon may vary between insecticide susceptible and field-collected strains, due to the latter's decreased energy allocation in reproduction in the presence of insecticide resistance. in this study, we evaluated the effect of chlorpyrifos (cp) and temephos (tp) exposure on the oviposition and survival of aedes aegypti (linnaeus) (diptera: culicidae) using a susceptible strain (khsm) and tw ...201829040719
detection and serotyping of dengue viruses in aedes aegypti and aedes albopictus (diptera: culicidae) collected in surabaya, indonesia from 2008 to 2015.aedes aegypti and aedes albopictus are the primary and secondary vectors, respectively, of dengue, the most important arboviral disease in the world. the aim of this study was to detect and serotype dengue viruses (denv) in the vectors ae. aegypti and ae. albopictus in surabaya, indonesia. between 2008 and 2015, 16,605 aedes mosquitoes were collected in 15 sub-districts of surabaya. ae. aegypti was dominant (90.9%), whereas few ae. albopictus were collected (9.1%). a total of 330 pools of adult ...201829093315
gold nanoparticles - against parasites and insect vectors.nanomaterials are currently considered for many biological, biomedical and environmental purposes, due to their outstanding physical and chemical properties. the synthesis of gold nanoparticles (au nps) is of high interest for research in parasitology and entomology, since these nanomaterials showed promising applications, ranging from detection techniques to drug development, against a rather wide range of parasites of public health relevance, as well as on insect vectors. here, i reviewed curr ...201829092797
sublethal exposure to deltamethrin reduces the abilities of giant water bugs to prey upon aedes aegypti larvae.freshwater ecosystems provide environmental conditions for many arthropod species, including pests like mosquitoes and beneficial insects. giant water bugs, belostoma anurum (hemiptera: belostomatidae), are aquatic insects that provide biological control of mosquitoes and small vertebrates in freshwater environments. however, the application of insecticides aiming to control mosquitoes can lead to insecticide exposures of aquatic predators that can result in their death or significant reductions ...201829049958
functional characterization of a serine protease inhibitor modulated in the infection of the aedes aegypti with dengue virus.during feeding with blood meal, female aedes aegypti can transmit infectious agents, such as dengue, yellow fever, chikungunya and zika viruses. dengue virus causes human mortality in tropical regions of the world, and there is no specific treatment or vaccine with maximum efficiency being used for these infections. in the vector-virus interaction, the production of several molecules is modulated by both mosquitoes and invading agents. however, little information is available about these molecul ...201829133118
zika virus: what we need to know?zika virus is one of the emerging viruses and is of significant threat to human health globally. it is a mosquito borne flavivirus similar to dengue, yellow fever, and west nile viruses. it was reported about 5 decades ago and then it spreads to different parts of the world. large outbreaks were reported on yap islands in 2007. now it has gained wide attention globally by health communities. major vector for virus transmission is aedes aegypti mosquito. zikv infection is mostly asymptomatic but ...201829131357
the bacterium wolbachia exploits host innate immunity to establish a symbiotic relationship with the dengue vector mosquito aedes aegypti.a host's immune system plays a central role in shaping the composition of the microbiota and, in return, resident microbes influence immune responses. symbiotic associations of the maternally transmitted bacterium wolbachia occur with a wide range of arthropods. it is, however, absent from the dengue and zika vector mosquito aedes aegypti in nature. when wolbachia is artificially forced to form symbiosis with this new mosquito host, it boosts the basal immune response and enhances the mosquito's ...201829099491
an overview of mosquito vectors of zika virus.the mosquito-borne arbovirus zika virus (zikv, flavivirus, flaviviridae), has caused an outbreak impressive by its magnitude and rapid spread. first detected in uganda in africa in 1947, from where it spread to asia in the 1960s, it emerged in 2007 on the yap island in micronesia and hit most islands in the pacific region in 2013. subsequently, zikv was detected in the caribbean, and central and south america in 2015, and reached north america in 2016. although zikv infections are in general asy ...201829481868
vector competence of selected north americananophelesandculexmosquitoes for zika virus.zika virus (zikv) is a vector-borne flavivirus that has caused recent outbreaks associated with serious disease in infants and newborns in the americas.aedesmosquitoes are the primary vectors for zikv, but little is known about the diversity of mosquitoes that can transmit zikv in north america. we chose three abundant north american mosquito species (anopheles freeborni,anopheles quadrimaculatus, andculex tarsalis) and one known vector species (aedes aegypti), fed them blood meals supplemented ...201829472998
potential occurrence of zika from subtropical to temperate argentina considering the basic reproduction number (r0).to assess the potential occurrence of zika transmission throughout argentina by the mosquito aedes aegypti considering the basic reproduction number (r0).201829466517
alterations of mitochondrial electron transport chain and oxidative stress induced by alkaloid-like α-aminonitriles on aedes aegypti larvae.aedes aegypti mosquitoes are responsible for dengue, chikungunya, and zika virus transmission in tropical and subtropical areas around the world. due to the absence of vaccines or antiviral drugs for human treatment, the majority of control strategies are targeted at ae. aegypti elimination. our research on mosquito control insecticidal agents has previously shown that the alkaloid girgensohnine and its analogues (α-aminonitriles) present in vitro acetylcholinesterase inhibition and in vivo inse ...201829463410
synergistic effect of entomopathogenic fungus fusarium oxysporum extract in combination with temephos against three major mosquito vectors.mosquito control using chemical insecticides is facing a major challenge due to development of insecticide resistance. improving the efficiency of existing insecticides using synergistic secondary metabolites of biological origin is increasingly being researched. herein, we evaluated the toxicity of fusarium oxysporum extract alone and in binary combinations with temephos, on larvae and pupae of anopheles stephensi, aedes aegypti and culex quinquefaciatus. f. oxysporum extract was characterized ...201829457957
modeling the spread of the zika virus using topological data analysis.zika virus (zikv), a disease spread primarily through the aedes aegypti mosquito, was identified in brazil in 2015 and was declared a global health emergency by the world health organization (who). epidemiologists often use common state-level attributes such as population density and temperature to determine the spread of disease. by applying techniques from topological data analysis, we believe that epidemiologists will be able to better predict how zikv will spread. we use the vietoris-rips fi ...201829438377
integrated vector control of aedes aegypti mosquitoes around target houses.the developing fetuses of pregnant women are at high risk of developing serious birth defects following zika virus infections. we applied an integrated vector control (ivc) approach using source reduction, larviciding, and mass trapping with non-insecticidal sticky traps to protect targeted houses by reducing the density of female aedes aegypti mosquitoes.201829422087
zika and aedes aegypti: new and old challenges.infection with the zika virus had a great impact not only on pregnant women and newborns, but also on public health, on popular ideas about aedes aegypti and with respect to women's social rights. the objective of this paper is to identify this impact and the historical, social and health changes of the disease and the legacy of the zika virus. interventions by researchers from different specialties foster conditions for more comprehensive investigations into future epidemic threats in brazil an ...201829412263
zika virus mb16-23 in mosquitoes, miami-dade county, florida, usa, 2016.we isolated a strain of zika virus, mb16-23, from aedes aegypti mosquitoes collected in miami beach, florida, usa, on september 2, 2016. phylogenetic analysis suggests that mb16-23 most likely originated from the caribbean region.201829400646
mission accomplished? we need a guide to the 'post release' world of wolbachia for aedes-borne disease control.historically, sustained control of aedes aegypti, the vector of dengue, chikungunya, yellow fever, and zika viruses, has been largely ineffective. subsequently, two novel 'rear and release' control strategies utilizing mosquitoes infected with wolbachia are currently being developed and deployed widely. in the incompatible insect technique, male aedes mosquitoes, infected with wolbachia, suppress populations through unproductive mating. in the transinfection strategy, both male and female wolbac ...201829396201
Displaying items 13701 - 13800 of 14974