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emergence of epidemic zika virus transmission and congenital zika syndrome: are recently evolved traits to blame?the mechanisms responsible for the dramatic emergence of zika virus (zikv), accompanied by congenital zika syndrome and guillain-barré syndrome (gbs), remain unclear. however, two hypotheses are prominent: (i) evolution for enhanced urban transmission via adaptation to mosquito vectors, or for enhanced human infection to increase amplification, or (ii) the stochastic introduction of zikv into large, naive human populations in regions with abundant aedes aegypti populations, leading to enough rar ...201728074023
fatty acid synthase 2 contributes to diapause preparation in a beetle by regulating lipid accumulation and stress tolerance genes expression.diapause, also known as dormancy, is a state of arrested development that allows insects to survive unfavorable environmental conditions. diapause-destined insects store large amounts of fat when preparing for diapause. however, the extent to which these accumulated fat reserves influence diapause remains unclear. to address this question, we investigated the function of fatty acid synthase (fas), which plays a central role in lipid synthesis, in stress tolerance, the duration of diapause prepar ...201728071706
dengue in latin america: systematic review of molecular epidemiological trends.dengue, the predominant arthropod-borne viral disease affecting humans, is caused by one of four distinct serotypes (denv-1, -2, -3 or -4). a literature analysis and review was undertaken to describe the molecular epidemiological trends in dengue disease and the knowledge generated in specific molecular topics in latin america, including the caribbean islands, from 2000 to 2013 in the context of regional trends in order to identify gaps in molecular epidemiological knowledge and future research ...201728068335
proteomic analysis of cry2aa-binding proteins and their receptor function in spodoptera exigua.the bacterium bacillus thuringiensis produces crystal (cry) proteins that are toxic to a diverse range of insects. transgenic crops that produce bt cry proteins are grown worldwide because of their improved resistance to insect pests. although bt "pyramid" cotton that produces both cry1a and cry2a is predicted to be more resistant to several lepidopteran pests, including spodoptera exigua, than plants that produce cry1ac alone, the mechanisms responsible for the toxicity of cry2aa in s. exigua a ...201728067269
wolbachia infections in aedes aegypti differ markedly in their response to cyclical heat stress.aedes aegypti mosquitoes infected with wolbachia bacteria are currently being released for arbovirus suppression around the world. their potential to invade populations and persist will depend on interactions with environmental conditions, particularly as larvae are often exposed to fluctuating and extreme temperatures in the field. we reared ae. aegypti larvae infected with different types of wolbachia (wmel, walbb and wmelpop-cla) under diurnal cyclical temperatures. rearing wmel and wmelpop-c ...201728056065
the dangers of accepting a single diagnosis: case report of concurrent plasmodium knowlesi malaria and dengue infection.dengue and malaria are two common, mosquito-borne infections, which may lead to mortality if not managed properly. concurrent infections of dengue and malaria are rare due to the different habitats of its vectors and activities of different carrier mosquitoes. the first case reported was in 2005. since then, several concurrent infections have been reported between the dengue virus (denv) and the malaria protozoans, plasmodium falciparum and plasmodium vivax. symptoms of each infection may be mas ...201728049485
pterodon emarginatus oleoresin-based nanoemulsion as a promising tool for culex quinquefasciatus (diptera: culicidae) control.preparation of nanoformulations using natural products as bioactive substances is considered very promising for innovative larvicidal agents. on this context, oil in water nanoemulsions develop a main role, since they satisfactorily disperse poor-water soluble substances, such as herbal oils, in aqueous media. pterodon emarginatus, popularly known as sucupira, has a promising bioactive oleoresin. however, to our knowledge, no previous studies were carried out to evaluate its potential against cu ...201728049483
insecticidal toxicities of carvacrol and thymol derived from thymus vulgaris lin. against pochazia shantungensis chou &lu., newly recorded pest.the insecticidal toxicities of five essential oils against pochazia shantungensis adults and nymphs, newly recorded pests, were evaluated. the lc50 values of thymus vulgaris, ruta graveolens, citrus aurantium, leptospermum petersonii and achillea millefolium oils were recorded as 57.48, 84.44, 92.58, 113.26 and 125.78 mg/l, respectively, against p. shantungensis nymphs using the leaf dipping bioassay, and 75.80, 109.86, 113.26, 145.06 and 153.74 mg/l, respectively, against p. shantungensis adult ...201728106093
rna interference mediated knockdown of brugia malayi udp-galactopyranose mutase severely affects parasite viability, embryogenesis and in vivo development of infective larvae.galactofuranose is an essential cell surface component present in bacteria, fungi and several nematodes such as caenorhabditis spp., brugia spp., onchocerca spp. and strongyloides spp. this sugar maintains the integrity of parasite surface and is essential for virulence. udp-galactopyranose mutase (bmugm) plays a key role in galf biosynthesis by catalyzing conversion of udp-galactopyranose into udp-galactofuranose and knockout studies of the gene in leishmania major, mycobacterium and aspergillu ...201728103957
antiviral effect of compounds derived from the seeds of mammea americana and tabernaemontana cymosa on dengue and chikungunya virus infections.the transmission of dengue virus (denv) and chikungunya virus (chikv) has increased worldwide, due in part to the lack of a specific antiviral treatment. for this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research priority. the objective of this study was to identify some of the compounds that are present in mammea americana (m. americana) and tabernaemontana cymosa (t. cymosa) plants and, subsequently, to evaluate their cyto ...201728100218
microrna-34 directly targets pair-rule genes and cytoskeleton component in the honey bee.micrornas (mirnas) are key regulators of developmental processes, such as cell fate determination and differentiation. previous studies showed dicer knockdown in honeybee embryos disrupt the processing of functional mature mirnas and impairs embryo patterning. here we investigated the expression profiles of mirnas in honeybee embryogenesis and the role of the highly conserved mir-34-5p in the regulation of genes involved in insect segmentation. a total of 221 mirnas were expressed in honey bee e ...201728098233
the physical genome mapping of anopheles albimanus corrected scaffold misassemblies and identified interarm rearrangements in genus anopheles.the genome of the neotropical malaria vector anopheles albimanus was sequenced as part of the 16 anopheles genomes project published in 2015. the draft assembly of this species consisted of 204 scaffolds with an n50 scaffold size of 18.1 mb and a total assembly size of 170.5 mb. it was among the smallest genomes with the longest scaffolds in the 16 anopheles species cluster, making an. albimanus the logical choice for anchoring the genome assembly to chromosomes. in this study, we developed a hi ...201727821634
a comparative analysis of three vector-borne diseases across australia using seasonal and meteorological models.ross river virus (rrv), barmah forest virus (bfv), and dengue are three common mosquito-borne diseases in australia that display notable seasonal patterns. although all three diseases have been modeled on localized scales, no previous study has used harmonic models to compare seasonality of mosquito-borne diseases on a continent-wide scale. we fit poisson harmonic regression models to surveillance data on rrv, bfv, and dengue (from 1993, 1995 and 1991, respectively, through 2015) incorporating s ...201728071683
mitigating future avian malaria threats to hawaiian forest birds from climate change.avian malaria, transmitted by culex quinquefasciatus mosquitoes in the hawaiian islands, has been a primary contributor to population range limitations, declines, and extinctions for many endemic hawaiian honeycreepers. avian malaria is strongly influenced by climate; therefore, predicted future changes are expected to expand transmission into higher elevations and intensify and lengthen existing transmission periods at lower elevations, leading to further population declines and potential extin ...201728060848
the search for novel insecticide targets in the post-genomics era, with a specific focus on g-protein coupled receptors.insects are considered pests globally, implicated in the destruction of agricultural fields and transmission of pathogens that cause deadly human diseases, such as dengue, zika and malaria. the diversity of the insecticide arsenal has remained stagnant for decades, but the recent rise of insecticide resistance fueled the discovery of novel modes of action, and the power of genomics has reinvigorated this search. this review discusses the importance of comparative and functional insect genomics i ...201728076467
the transcriptome of the mosquito aedes fluviatilis (diptera: culicidae), and transcriptional changes associated with its native wolbachia infection.wolbachia is a bacterial endosymbiont that naturally infects a wide range of insect species, and causes drastic changes to host biology. stable infections of wolbachia in mosquitoes can inhibit infection with medically important pathogens such as dengue virus and malaria-causing plasmodium parasites. however, some native wolbachia strains can enhance infection with certain pathogens, as is the case for the mosquito aedes fluviatilis, where infection with plasmodium gallinaceum is enhanced by the ...201728049478
argonaute 2 suppresses japanese encephalitis virus infection in aedes aegypti.there are three main innate immune mechanisms against viruses in mosquitoes. infection with the flavivirus dengue virus is controlled by rna interference (rnai) and the jak-stat and toll signaling pathways. this study showed that another flavivirus, japanese encephalitis virus (jev), did not invade the salivary glands of aedes aegypti and that this may be a result of the innate immune resistance to the virus. argonaute 2 (ago2) plays a critical role in the rnai pathway. to understand the mechani ...201727169949
magnetic nanoparticles are highly toxic to chloroquine-resistant plasmodium falciparum, dengue virus (den-2), and their mosquito vectors.a main challenge in parasitology is the development of reliable tools to prevent or treat mosquito-borne diseases. we investigated the toxicity of magnetic nanoparticles (mnp) produced by magnetospirillum gryphiswaldense (strain msr-1) on chloroquine-resistant (cq-r) and sensitive (cq-s) plasmodium falciparum, dengue virus (den-2), and two of their main vectors, anopheles stephensi and aedes aegypti, respectively. mnp were studied by fourier-transform infrared spectroscopy and transmission elect ...201727815736
predicting arboviral disease emergence using bayesian networks: a case study of dengue virus in western australia.a bayesian belief network (bbn) for assessing the potential risk of dengue virus emergence and distribution in western australia (wa) is presented and used to identify possible hotspots of dengue outbreaks in summer and winter. the model assesses the probabilities of two kinds of events which must take place before an outbreak can occur: (1) introduction of the virus and mosquito vectors to places where human population densities are high; and (2) vector population growth rates as influenced by ...201727620510
molecular identification of aedes aegypti mosquitoes from pilani region of rajasthan, india.aedes aegypti is the most important vector of dengue virus infection in humans worldwide. accurate identification and colonization are the essential requirements to understand vector biology as well as its diseases transmission potential. in this study, we have used molecular approaches for the identification of ae. aegypti mosquitoes that were collected from the pilani region of rajasthan, india methods: field collected mosquito larvae were colonized under laboratory conditions. conserved genet ...201727353585
zika virus and neuroscience: the need for a translational collaboration.zika virus (zikv) has become a major challenge for scientists and health agencies. zikv's involvement with human fetal microcephaly and guillain-barré syndrome and its transmission through aedes africanus and aedes aegypti mosquitos highlighted the epidemiological and neurological risks associated to zikv infection. in 2013, zikv arrives in brazil but the first outbreak in the country was reported in 2015. here, we used the web of science as a search tool for comparing the evolution of world and ...201728185126
a heparin-functionalized carbon nanotube-based affinity biosensor for dengue virus.dengue virus is an arthropod-borne virus transmitted primarily by aedes mosquitos and is major cause of disease in tropical and subtropical regions. colloquially known as dengue fever, infection can cause hemorrhagic disorders and death in humans and non-human primates. we report a novel electronic biosensor based on a single-walled carbon nanotube network chemiresistive transducer that is functionalized with heparin for low-cost, label-free, ultra-sensitive, and rapid detection of whole dengue ...201728152487
dengue virus serotype 2 infection alters midgut and carcass gene expression in the asian tiger mosquito, aedes albopictus.the asian tiger mosquito, aedes albopictus is currently an important vector for dengue, chikungunya and zika virus, and its role in transmission of arthropod-borne viruses (arboviruses) may increase in the future due to its ability to colonize temperate regions. in contrast to aedes aegypti, the dominant vector of dengue, chikungunya and zika virus, genetic responses of ae. albopictus upon infection with an arbovirus are not well characterized. here we present a study of the changes in transcrip ...201728152011
superinfection interference between dengue-2 and dengue-4 viruses in aedes aegypti mosquitoes.dengue virus consists of four antigenically distinct serotypes (denv 1-4) that are transmitted to humans by aedes aegypti and aedes albopictus mosquitoes. in many dengue-endemic regions, co-circulation of two or more denv serotypes is fairly common increasing the likelihood for exposure of the two vectors to multiple serotypes. we used a model system of denv-2 and denv-4 to investigate how prior exposure of aedes aegypti to one denv serotype affects its susceptibility to another serotype.201728150899
the transovarial transmission in the dynamics of dengue infection: epidemiological implications and thresholds.the anthropophilic and peridomestic female mosquito aedes aegypti bites humans to suck blood to maturate fertilized eggs, during which dengue virus can be spread between mosquito and human populations. besides this route of transmission, there is a possibility of dengue virus being passed directly to offspring through transovarial (or vertical) transmission. the effects of both horizontal and transovarial transmission routes on the dengue virus transmission are assessed by mathematical modeling. ...201728131789
recent advances in the identification of the host factors involved in dengue virus replication.dengue virus (denv) belongs to the genus flavivirus of the family flaviviridae and it is primarily transmitted via aedes aegypti and aedes albopictus mosquitoes. the life cycle of denv includes attachment, endocytosis, protein translation, rna synthesis, assembly, egress, and maturation. recent researches have indicated that a variety of host factors, including cellular proteins and micrornas, positively or negatively regulate the denv replication process. this review summarizes the latest findi ...201728124222
aedes aegypti (diptera: culicidae) longevity and differential emergence of dengue fever in two cities in sonora, mexico.dengue virus, primarily transmitted by the aedes aegypti (l.) mosquito, has rapidly expanded in geographic extent over the past several decades. in some areas, however, dengue fever has not emerged despite established ae. aegypti populations. the reasons for this are unclear and have sometimes been attributed to socio-economic differences. in 2013 we compared ae. aegypti adult density and population age structure between two cities in sonora, mexico: hermosillo, which has regular seasonal dengue ...201728082648
comparison of stable and transient wolbachia infection models in aedes aegypti to block dengue and west nile viruses.pathogen replication and transmission in wolbachia infected insects are currently studied using three wolbachia infection systems: naturally infected wolbachia hosts, hosts transinfected with wolbachia (stably maintained and inherited infections) and hosts transiently infected with wolbachia. all three systems have been used to test the effect of wolbachia on mosquito transmitted pathogens such as dengue virus (denv), west nile virus (wnv) and plasmodium. from these studies it is becoming increa ...201728052065
an analysis of the potential impact of climate change on dengue transmission in the southeastern united states.dengue fever, caused by a mosquito-transmitted virus, is an increasing health concern in the americas. meteorological variables such as temperature and precipitation can affect disease distribution and abundance through biophysical impacts on the vector and on the virus. such tightly coupled links may facilitate further spread of dengue fever under a changing climate. in the southeastern united states, the dengue vector is widely established and exists on the current fringe of dengue transmissio ...201727713106
the dna chaperone hmgb1 potentiates the transcriptional activity of rel1a in the mosquito aedes aegypti.high mobility group protein 1 (hmgb1) is a non-histone, chromatin-associated nuclear protein that functions in regulating eukaryotic gene expression. we investigated the influence and mechanism of action of aedes aegypti hmgb1 (aahmgb1) on mosquito rel1a-mediated transcription from target gene promoters. the dna-binding domain (rhd) of aarel1a was bacterially expressed and purified, and aahmgb1 dramatically enhanced rhd binding to consensus nf-kb/rel dna response elements. luciferase reporter an ...201727867076
optimal control problems of mosquito-borne disease subject to changes in feeding behavior of aedes mosquitoes.dengue viruses (denv) are transmitted to humans by the bite of aedes mosquitoes. it is known that dengue virus infection in aedes aegypti female mosquitoes makes a change in the feeding behavior of the infected mosquitoes. in this study, using the forces of infection, we incorporated the effect of changes in the feeding behavior of mosquitoes into the standard vector-borne sir-si model. it has been proved that both a single-strain model and a two-strain model exhibit forward bifurcations. moreov ...201728385591
catalase protects aedes aegypti from oxidative stress and increases midgut infection prevalence of dengue but not zika.digestion of blood in the midgut of aedes aegypti results in the release of pro-oxidant molecules that can be toxic to the mosquito. we hypothesized that after a blood meal, the antioxidant capacity of the midgut is increased to protect cells against oxidative stress. concomitantly, pathogens present in the blood ingested by mosquitoes, such as the arboviruses dengue and zika, also have to overcome the same oxidative challenge, and the antioxidant program induced by the insect is likely to influ ...201728379952
the relationship between entomological indicators of aedes aegypti abundance and dengue virus infection.routine entomological monitoring data are used to quantify the abundance of ae. aegypti. the public health utility of these indicators is based on the assumption that greater mosquito abundance increases the risk of human denv transmission, and therefore reducing exposure to the vector decreases incidence of infection. entomological survey data from two longitudinal cohort studies in iquitos, peru, linked with 8,153 paired serological samples taken approximately six months apart were analyzed. i ...201728333938
a new paradigm for aedes spp. surveillance using gravid ovipositing sticky trap and ns1 antigen test kit.dengue remains a serious public health problem in southeast asia and has increased 37-fold in malaysia compared to decades ago. new strategies are urgently needed for early detection and control of dengue epidemics.201728327173
biologic evidence required for zika disease enhancement by dengue antibodies.the sudden appearance of overt human zika virus infections that cross the placenta to damage fetal tissues, target sexual organs, and are followed in some instances by guillain-barré syndrome raises questions regarding whether these outcomes are caused by genetic mutations or if prior infection by other flaviviruses affects disease outcome. because dengue and zika viruses co-circulate in the urban aedes aegypti mosquito-human cycle, a logical question, as suggested by in vitro data, is whether d ...201728322690
occurrence of concurrent infections with multiple serotypes of dengue viruses during 2013-2015 in northern kerala, india.dengue is a global human public health threat, causing severe morbidity and mortality. the occurrence of sequential infection by more than one serotype of dengue virus (denv) is a major contributing factor for the induction of dengue hemorrhagic fever (dhf) and dengue shock syndrome (dss), two major medical conditions caused by denv infection. however, there is no specific drug or vaccine available against dengue infection. there are reports indicating the increased incidence of concurrent infec ...201728316881
dengue virus ns2b/ns3 protease inhibitors exploiting the prime side.the mosquito-transmitted dengue virus (denv) infects millions of people in tropical and sub-tropical regions. maturation of denv particles requires proper cleavage of the viral polyprotein, including processing 8 of the 13 substrate cleavage sites by dengue ns2b-s3 protease. with no available direct-acting antiviral targeting denv, ns2-s3 protease is a promising target for inhibitor design. current design efforts focus on the nonprime side of the denv protease active site, resulting in highly hy ...201728298600
the microbiome composition of aedes aegypti is not critical for wolbachia-mediated inhibition of dengue virus.dengue virus (denv) is primarily vectored by the mosquito aedes aegypti, and is estimated to cause 390 million human infections annually. a novel method for denv control involves stable transinfection of ae. aegypti with the common insect endosymbiont wolbachia, which mediates an antiviral effect. however, the mechanism by which wolbachia reduces the susceptibility of ae. aegypti to denv is not fully understood. in this study we assessed the potential of resident microbiota, which can play impor ...201728267749
the rnai pathway plays a small part in wolbachia-mediated blocking of dengue virus in mosquito cells.wolbachia pipientis is an insect endosymbiont known to limit the replication of viruses including dengue and zika in their primary mosquito vector, aedes aegypti. wolbachia is being released into mosquito populations globally in a bid to control the diseases caused by these viruses. it is theorized that wolbachia's priming of the insect immune system may confer protection against subsequent viral infection. other hypotheses posit a role for competition between wolbachia and viruses for host cell ...201728262718
population genomics reveals that an anthropophilic population of aedes aegypti mosquitoes in west africa recently gave rise to american and asian populations of this major disease vector.the mosquito aedes aegypti is the main vector of dengue, zika, chikungunya and yellow fever viruses. this major disease vector is thought to have arisen when the african subspecies ae. aegypti formosus evolved from being zoophilic and living in forest habitats into a form that specialises on humans and resides near human population centres. the resulting domestic subspecies, ae. aegypti aegypti, is found throughout the tropics and largely blood-feeds on humans.201728241828
[chikungunya, an emerging viral disease. proposal of an algorithm for its clinical management].chikungunya fever (chik) is an emerging viral disease. it is caused by the chikungunya virus, an alphavirus from the togaviridae family. it is transmitted to humans by the bite of infected mosquitoes, mainly aedes aegypti and aedes albopictus. they are also involved in the transmission of dengue, malaria, etc. chik is now endemic in any region of africa and southeast-asia. cases of chik have been reported in america, the caribbean, and europe (france, italy and spain). there are reservoirs of th ...201725440971
[technical guidelines for the prevention and treatment of chikungunya fever].chikungunya fever is an emerging disease caused by an alphavirus belonging to the togaviridae family, transmitted by the bite of aedes genus species: aedesaegypti and aedesalbopictus. in 2013, paho/who received confirmation of the first cases of indigenous transmission of chikungunya in the americas. this disease may be acute, subacute and chronic, affecting all age groups. following an incubation period from three to seven days, the patient usually begins with a high fever (greater than 39 °c), ...201725680650
potential high-risk areas for zika virus transmission in the contiguous united states.to understand where transmission of zika virus has the highest likelihood to occur in the contiguous united states with regard to its transmission both sexually and via aedes aegypti mosquito bites.201728323468
development of dirofilaria immitis and dirofilaria repens in aedes japonicus and aedes geniculatus.the mosquito-borne filarial nematodes dirofilaria immitis and dirofilaria repens primarily affect dogs but also cats, causing heartworm disease or subcutaneous dirofilariosis, respectively, and both may also cause zoonotic diseases in humans. several mosquito species have been reported as competent vectors for these nematodes, but no data are available for the invasive mosquito species aedes japonicus (theobald, 1901). the objective of this study was to describe the development of both d. immiti ...201728219407
first case of zika virus infection in a hiv+ patient.zika virus is an enveloped, single-stranded rna virus that belongs to the flavivirus genre. as with arbovirus, it is transmitted by arthropods (mosquitoes), both aedes aegypti (urban) and albopictus (rural). zika virus was first isolated in 1947 from monkeys in uganda. the first human cases were reported in 1952 in east africa. outbreaks were reported in micronesia (2007), polynesia (2013), and chile (2014). both diagnostic procedures and therapeutics are poorly developed. serological tests cros ...201727232506
mosgctl-7, a c-type lectin protein, mediates japanese encephalitis virus infection in mosquitoes.japanese encephalitis virus (jev) is an arthropod-borne flavivirus prevalent in asia and the western pacific, and is the leading cause of viral encephalitis. jev is maintained in a transmission cycle between mosquitoes and vertebrate hosts, but the molecular mechanisms by which the mosquito vector participates in transmission are unclear. we investigated the expression of all c-type lectins during jev infection in aedes aegypti. c-type lectin mosgctl-7 (aael002524) was significantly up-regulated ...201728250133
n-glycosylation influences the catalytic activity of mosquito α-glucosidases associated with susceptibility or refractoriness to lysinibacillus sphaericus.cqm1 and aam1 are α-glucosidases (ec 3.2.1.20) expressed in culex quinquefasciatus and aedes aegypti larvae midgut, respectively. these orthologs share high sequence similarity but while cqm1 acts as a receptor for the binary (bin) insecticidal toxin from lysinibacillus sphaericus, aam1 does not bind the toxin, rendering ae. aegypti refractory to this bacterium. aam1 is heavily glycosylated, contrasting to cqm1, but little is known regarding how glycosylation impacts on its function. this study ...201728017798
chemical composition and in vitro antioxidant, cytotoxic, antimicrobial, and larvicidal activities of the essential oil of mentha piperita l. (lamiaceae).the essential oil was obtained by hydrodistillation and the identification and quantification of components were achieved with the use of gc-ms analysis. the antioxidant activity was evaluated by the method of sequestration of dpph. essential oils were used for study the cytotoxic front larvae of artemia salina. in the evaluation of the antimicrobial activity of essential oils, we employed the disk-diffusion method. the potential larvicide in mosquito larvae of the third stage of development of ...201728116346
transcriptome sequencing reveals large-scale changes in axenic aedes aegypti larvae.mosquitoes host communities of microbes in their digestive tract that consist primarily of bacteria. we previously reported that aedes aegypti larvae colonized by a native community of bacteria and gnotobiotic larvae colonized by only escherichia coli develop very similarly into adults, whereas axenic larvae never molt and die as first instars. in this study, we extended these findings by first comparing the growth and abundance of bacteria in conventional, gnotobiotic, and axenic larvae during ...201728060822
larvicidal and growth-inhibitory activity of entomopathogenic bacteria culture fluids against aedes aegypti (diptera: culicidae).dengue, chikungunya, and zika are important vector-borne diseases, and aedes aegypti l. is their main transmitter. as the disease management is mainly based on mosquito control strategies, the search for alternative and cost-effective approaches is ongoing. the gram-negative bacteria xenorhabdus nematophila and photorhabdus luminescens are symbiotically associated with entomopathogenic nematodes and are highly pathogenic for insect larvae. after we have recently confirmed the toxicity of these b ...201728062794
artesunate-tafenoquine combination therapy promotes clearance and abrogates transmission of the avian malaria parasite plasmodium gallinaceum.clinical manifestations of malaria infection in vertebrate hosts arise from the multiplication of the asexual stage parasites in the blood, while the gametocytes are responsible for the transmission of the disease. antimalarial drugs that target the blood stage parasites and transmissible gametocytes are rare, but are essentially needed for the effective control of malaria and for limiting the spread of resistance. artemisinin and its derivatives are the current first-line antimalarials that are ...201728043395
the evolutionary divergence of stat transcription factor in different anopheles species.anopheles mosquito transmits plasmodium, the malaria causing parasite. different species of anopheles mosquito dominate in a particular geographical location and are capable of transmitting specific strains of plasmodium. it is important to understand the biology of different anophelines to control the parasite transmission. stat is an evolutionary conserved transcription factor that regulates the parasite development in african malaria vector anopheles gambiae. unlike drosophila and aedes aegyp ...201727664587
molecular expression of aquaporin mrnas in the northern house mosquito, culex pipiens.aquaporins (aqps) are a family of membrane-bound proteins, originally described as water-channels, that broadly exist in prokaryotic and eukaryotic cells. however, some aqps can transport small molecules (e.g. urea, glycerol) along with or preferentially to water. previous work in the mosquitoes aedes aegypti and anopheles gambiae have characterized the molecular expression of one or more aqp genes and shown that they are involved in water homeostasis after blood feeding, tolerance to dehydratio ...201727773635
toxicity of herbal extracts used in ethno-veterinary medicine and green-encapsulated zno nanoparticles against aedes aegypti and microbial pathogens.dengue and chikungunya are arboviral diseases mainly vectored by the mosquito aedes aegypti. presently, there is no treatment for these viral diseases and their prevention is still based on vector control measures. nanopesticides fabricated using herbal extracts as reducing and capping agents currently represent an excellent platform for pest control. in this scenario, the present study assessed the acute toxicity of seven plants employed in ethno-veterinary medicine of southern india, as well a ...201728389893
pseudomonas aeruginosa kun2, extracellular toxins-a potential source for the control of dengue vector.dengue fever is one of the serious health disease transmitted by aedes spp mosquitoes. the incidence of dengue has increased dramatically around the world in recent decades. vector control is one of the important strategies practiced for the control of dengue fever. the emergence of resistance among vectors against the existing insecticides has raised new challenges. the aim of the present study was to identify the larvicidal activity of extracellular toxins from pseudomonas spp for the control ...201727353579
microbiota activates imd pathway and limits sindbis infection in aedes aegypti.aedes aegypti is the main vector of important arboviruses such as dengue, zika and chikungunya. during infections mosquitoes can activate the immune pathways toll, imd and jak/stat to limit pathogen replication.201728231846
rplp1 and rplp2 are essential flavivirus host factors that promote early viral protein accumulation.the flavivirus genus contains several arthropod-borne viruses that pose global health threats, including dengue viruses (denv), yellow fever virus (yfv), and zika virus (zikv). in order to understand how these viruses replicate in human cells, we previously conducted genome-scale rna interference screens to identify candidate host factors. in these screens, we identified ribosomal proteins rplp1 and rplp2 (rplp1/2) to be among the most crucial putative host factors required for denv and yfv infe ...201727974556
diversity of yellow fever mosquito vectors in the atlantic forest of rio de janeiro, brazil.environmental modifications caused by human activities have led to changes in mosquito vector populations, and sylvatic species have adapted to breeding in urban areas.201727384833
modelling wolbachia infection in a sex-structured mosquito population carrying west nile virus.wolbachia is possibly the most studied reproductive parasite of arthropod species. it appears to be a promising candidate for biocontrol of some mosquito borne diseases. we begin by developing a sex-structured model for a wolbachia infected mosquito population. our model incorporates the key effects of wolbachia infection including cytoplasmic incompatibility and male killing. we also allow the possibility of reduced reproductive output, incomplete maternal transmission, and different mortality ...201728097419
spatial quantification of the world population potentially exposed to zika virus.zika virus is an emerging flaviviridae virus, which has spread rapidly in the last few years. it has raised concern because it has been associated with fetus microcephaly when pregnant women are infected. the main vector is the mosquito aedes aegypti , distributed in tropical areas.201728338754
boswellia ovalifoliolata (burseraceae) essential oil as an eco-friendly larvicide? toxicity against six mosquito vectors of public health importance, non-target mosquito fishes, backswimmers, and water bugs.the use of synthetic pesticides to control vector populations is detrimental to human health and the environment and may lead to the development of resistant strains. plants can be alternative sources of safer compounds effective on mosquito vectors. in this study, the mosquito larvicidal activity of boswellia ovalifoliolata leaf essential oil (eo) was evaluated against anopheles stephensi, anopheles subpictus, aedes aegypti, aedes albopictus, culex quinquefasciatus, and culex tritaeniorhynchus. ...201728332087
ascension island: a survey to assess the presence of zika virus vectors.following concerns regarding the spread of zika virus, joint services health unit (cyprus) were tasked to carry out a mosquito survey on the ascension island, south atlantic. this was to determine if vectors of the virus such as aedes aegypti and aedes albopictus were introduced and established on the island.201728331047
spatial-temporal analysis of dengue deaths: identifying social vulnerabilities.currently, dengue fever, chikungunya fever, and zika virus represent serious public health issues in brazil, despite efforts to control the vector, the aedes aegypti mosquito.201728327810
correction: laboratory strains of aedes aegypti are competent to brazilian zika virus.[this corrects the article doi: 10.1371/journal.pone.0171951.].201728288192
variation in aedes aegypti mosquito competence for zika virus transmission.to test whether zika virus has adapted for more efficient transmission by aedes aegypti mosquitoes, leading to recent urban outbreaks, we fed mosquitoes from brazil, the dominican republic, and the united states artificial blood meals containing 1 of 3 zika virus strains (senegal, cambodia, mexico) and monitored infection, dissemination, and virus in saliva. contrary to our hypothesis, cambodia and mexica strains were less infectious than the senegal strain. only mosquitoes from the dominican re ...201728287375
<i>aedes aegypti</i> control through modernized, integrated vector management.in the context of the ongoing, unprecedented zika virus outbreak in the americas, the world health organization has expressed its support for developing and up-scaling three novel approaches to controlling the aedes aegypti mosquito: the sterile insect technique (sit), the release of insects carrying dominant lethal genes (ridl) and the release of wolbachia-infected mosquitoes. whereas the former two approaches are temporary insect population suppression strategies, wolbachia infection is a self ...201728286698
vertical transmission of zika virus by aedes aegypti and ae. albopictus mosquitoes.to determine the potential role of vertical transmission in zika virus expansion, we evaluated larval pools of perorally infected aedes. aegypti and ae. albopictus adult female mosquitoes; ≈1/84 larvae tested were zika virus-positive; and rates varied among mosquito populations. thus, vertical transmission may play a role in zika virus spread and maintenance.201728277199
zika in rio de janeiro: assessment of basic reproduction number and comparison with dengue outbreaks.zika virus infection was declared a public health emergency of international concern in february 2016 in response to the outbreak in brazil and its suspected link with congenital anomalies. in this study, we use notification data and disease natural history parameters to estimate the basic reproduction number (r 0) of zika in rio de janeiro, brazil. we also obtain estimates of r 0 of dengue from time series of dengue cases in the outbreaks registered in 2002 and 2012 in the city, when denv-3 and ...201728240195
combining contact tracing with targeted indoor residual spraying significantly reduces dengue transmission.the widespread transmission of dengue viruses (denv), coupled with the alarming increase of birth defects and neurological disorders associated with zika virus, has put the world in dire need of more efficacious tools for aedes aegypti-borne disease mitigation. we quantitatively investigated the epidemiological value of location-based contact tracing (identifying potential out-of-home exposure locations by phone interviews) to infer transmission foci where high-quality insecticide applications c ...201728232955
leaking containers: success and failure in controlling the mosquito aedes aegypti in brazil.in 1958, the pan american health organization declared that brazil had successfully eradicated the mosquito aedes aegypti, responsible for the transmission of yellow fever, dengue fever, chikungunya, and zika virus. yet in 2016 the brazilian minister of health described the situation of dengue fever as "catastrophic." discussing the recent epidemic of zika virus, which amplified the crisis produced by the persistence of dengue fever, brazil's president declared in january 2016 that "we are in th ...201728207332
does zika virus infection affect mosquito response to repellents?the world health organization (who) recommends that people travelling to or living in areas with zika virus (zikv) outbreaks or epidemics adopt prophylactic measures to reduce or eliminate mosquito bites, including the use of insect repellents. it is, however, unknown whether repellents are effective against zikv-infected mosquitoes, in part because of the ethical concerns related to exposing a human subject's arm to infected mosquitoes in the standard arm-in-cage assay. we used a previously dev ...201728205633
a cdna clone-launched platform for high-yield production of inactivated zika vaccine.a purified inactivated vaccine (piv) using the zika virus (zikv) puerto rico strain prvabc59 showed efficacy in monkeys, and is currently in a phase i clinical trial. high-yield manufacture of this piv is essential for its development and vaccine access. here we report an infectious cdna clone-launched platform to maximize its yield. a single ns1 protein substitution (k265e) was identified to increase zikv replication on vero cells (a cell line approved for vaccine production) for both cambodian ...201728196656
laboratory strains of aedes aegypti are competent to brazilian zika virus.the zika virus outbreaks are unprecedented human threat in relation to congenital malformations and neurological/autoimmune complications. since this virus has high potential to spread in regions presenting the vectors, improvement in mosquito control is a top priority. thus, aedes aegypti laboratory strains will be fundamental to support studies in different research fields implicated on zika-mosquito interactions which are the basis for the development of innovative control methods. in this se ...201728187183
predicting the potential role of non-human hosts in zika virus maintenance.arboviruses are often maintained in complex cycles involving vertebrates such as mammals or birds and blood-feeding mosquitoes. however, the role of wildlife hosts in their emergence or re-emergence in human populations has received little attention. the recent emergence of zika virus in america, and previous occurrences of chikungunya and dengue, forces us to confront a potential new disease-emergence phenomenon. using a spatial data mining framework to identify potential biotic interactions, b ...201728180996
larvicidal activity of blumea eriantha essential oil and its components against six mosquito species, including zika virus vectors: the promising potential of (4e,6z)-allo-ocimene, carvotanacetone and dodecyl acetate.the effective and environmentally sustainable control of mosquitoes is a challenge of essential importance. this is due to the fact that some invasive mosquitoes, with special reference to the aedes genus, are particularly difficult to control, due to their high ecological plasticity. moreover, the indiscriminate overuse of synthetic insecticides resulted in undesirable effects on human health and non-target organisms, as well as resistance development in targeted vectors. here, the leaf essenti ...201728168560
toxicity of ar-curcumene and epi-β-bisabolol from hedychium larsenii (zingiberaceae) essential oil on malaria, chikungunya and st. louis encephalitis mosquito vectors.mosquitoes act as vectors of key pathogens and parasites. plant essential oils have been recognized as important sources of biopesticides, which do not induce resistance and have limited toxic effects on human health and non-target organisms. in this research, we evaluated the larvicidal and oviposition deterrence activity of hedychium larsenii essential oil (eo) and its major compounds ar-curcumene and epi-β-bisabolol. both molecules showed high toxicity against early third instars of anopheles ...201727918946
zika virus-what the otolaryngologist should know: a review.initially discovered in 1947, zika virus infection received little notoriety as a tropical disease until 2015 when an outbreak of microcephaly cases was reported in brazil. zika is a single-stranded rna arbovirus of the flaviviridae family. the primary source of infection in humans stems from aedes aegypti mosquito bites but can also occur through sexual, blood, and perinatal transmission. with expectations that 3 to 4 million people across the americas will be infected over the next year, the w ...201727892990
an integrated approach for the assessment of the aedes aegypti and aedes albopictus global spatial distribution, and determination of the zones susceptible to the development of zika virus.the zika virus, one of the new epidemic diseases, is reported to have affected millions of people in the past year. the suitable climate conditions of the areas where zika virus has been reported, especially in areas with a high population density, are the main cause of the current outbreak and spread of the disease. indeed, the suitable climatic conditions of certain territories constitute perfect breading nest for the propagation and outbreak of worldwide diseases. the main objective of this r ...201728111132
experimental transmission of zika virus by mosquitoes from central europe.mosquitoes collected in germany in 2016, including culex pipiens pipiens biotype pipiens, culex torrentium and aedes albopictus, as well as culex pipiens pipiens biotype molestus (in colony since 2011) were experimentally infected with zika virus (zikv) at 18 °c or 27 °c. none of the culex taxa showed vector competence for zikv. in contrast, aedes albopictus were susceptible for zikv but only at 27 °c, with transmission rates similar to an aedes aegypti laboratory colony tested in parallel.201728106528
zika virus vector competency of mosquitoes, gulf coast, united states.zika virus has recently spread throughout the americas. although aedes aegypti mosquitoes are considered the primary vector, culex quinquefasciatus and mosquitoes of other species may also be vectors. we tested cx. quinquefasciatus and ae. taeniorhynchus mosquitoes from the us gulf coast; both were refractory to infection and incapable of transmission.201728005002
zika virus in asia.zika virus (zikv) is an emerging mosquito-borne virus that was first isolated from a sentinel rhesus monkey in the zika forest in uganda in 1947. in asia, the virus was isolated in malaysia from aedes aegypti mosquitoes in 1966, and the first human infections were reported in 1977 in central java, indonesia. in this review, all reported cases of zikv infection in asia as of september 1, 2016 are summarized and some of the hypotheses that could currently explain the apparently low incidence of zi ...201727939768
flavivirus transmission focusing on zika.flaviviruses are among the most diverse viruses with over 85 species recognized. taxonomically, this genus is one of the 4 recognized genera within the family flaviviridae. most flaviviruses of human public health significance, for example, dengue, yellow fever and zika viruses, are arthropod-borne (arboviruses) and have two evolutionarily and ecologically distinct transmission cycles: a sylvatic transmission cycle, where the virus circulates between zoonotic vertebrate reservoir and amplificati ...201727936448
does zika virus cause microcephaly - applying the bradford hill viewpoints.zika virus has been documented since 1952, but been associated with mild, self-limiting disease. zika virus is classified as an arbovirus from a family of flaviviridae and primarily spread by aedes aegypti mosquitos. however, in a large outbreak in brazil in 2015, zika virus has been associated with microcephaly.201728357156
host-virus interaction of zika virus in modulating disease pathogenesis.the zika virus (zikv) is a newly emerging pathogen that has resulted in a worldwide epidemic. it primarily spreads either through infected aedes aegypti or aedes albopictus mosquitos leading to severe neurological disorders such as microcephaly and guillain-barré syndrome in susceptible individuals. the mode of zikv entry into specific cell types such as: epidermal keratinocytes, fibroblasts, immature dendritic cells (idcs), and stem-cell-derived human neural progenitors has been determined thro ...201728349242
[zika virus infection in pregnancy].the zika virus is a flavivirus spread by mosquitoes. its primary vectors are the aedes aegypti and the aedes albopictus. before 2007 it sporadically caused benign morbidity. since 2015, it started spreading "explosively" in america, especially in brazil. in august 2016 they reported cases from new york and poland, too. most of the infections don't produce any symptoms, but can cause grave complications. the most important lesion is microcephalia that forms in fetuses. microcephalia's most seriou ...201728393598
new records of aedes aegypti in southern oklahoma, 2016.aedes aegypti is an important subtropical vector species and is predicted to have a limited year-round distribution in the southern united states. collection of the species has not been officially verified in oklahoma since 1940. adult mosquitoes were collected in 42 sites across 7 different cities in oklahoma using 3 different mosquito traps between may and september 2016. between july and september 2016, 88 ae. aegypti adults were collected at 18 different sites in 4 different cities across so ...201728388329
entomological investigations during early stages of a chikungunya outbreak in the united states virgin islands, 2014.during the 2014 chikungunya (chik) outbreak in the caribbean, we performed entomological surveys on 3 united states virgin islands (usvi): st. croix, st. thomas, and st. john. we aimed to evaluate the potential for chikungunya virus (chikv) transmission in the usvi. the surveys took place between june 19, 2014, and june 29, 2014, during the dry season in usvi. a total of 1,929 adult mosquitoes belonging to 4 species- culex quinquefasciatus (68.4%), aedes aegypti (29.7%), ae. mediovittatus (1.3%) ...201728388320
potential of aedes aegypti and aedes albopictus populations in the central african republic to transmit enzootic chikungunya virus strains.major chikungunya outbreaks have affected several central african countries during the past decade. the chikungunya virus (chikv) was isolated from humans and sylvan mosquitoes in the central african republic (car) during the 1970 and 1980s but has not been found recently, despite the presence of aedes albopictus since 2010. the risk of a massive chikungunya epidemic is therefore potentially high, as the human populations are immunologically naïve and because of the presence of the mosquito vect ...201728347325
computer-aided structure based drug design approaches for the discovery of new anti-chikv agents.chikungunya is a viral infection caused by chikungunya virus (chikv), an arbovirus transmitted through mosquito (aedes aegypti and aedes albopictus) bite. the virus from sylvatic cycle in africa mutated to new vector adaptation and became one of the major emerging and re-emerging viral infections in the past decade, affecting more than 40 countries. efforts are being made by many researches to develop means to prevent and control the infection through vaccines and vector control strategy. on the ...201728294048
chikungunya infection: a global public health menace.chikungunya virus (chikv) has been involved in epidemics in african and asian subcontinents and, of late, has transcended to affect the americas. aedes aegypti and aedes albopictus are the major vectors for chikv infection, which results in dissemination of virus to various vital organs. entry of virus into these tissues causes infiltration of innate immune cells, monocytes, macrophages, neutrophils, natural killer cells, and adaptive immune cells. macrophages bearing the replicating virus, in t ...201728233156
aedes-borne virus-mosquito interactions: mass spectrometry strategies and findings.aedes-borne viruses are responsible for high-impact neglected tropical diseases and unpredictable outbreaks such as the ongoing zika epidemics. aedes mosquitoes spread different arboviruses such as dengue virus (denv), chikungunya virus (chikv), and zika virus, among others, and are responsible for the continuous emergence and reemergence of these pathogens. these viruses have complex transmission cycles that include two hosts, namely the aedes mosquito as a vector and susceptible vertebrate hos ...201728192064
environmental and social change drive the explosive emergence of zika virus in the americas.since zika virus (zikv) was detected in brazil in 2015, it has spread explosively across the americas and has been linked to increased incidence of microcephaly and guillain-barré syndrome (gbs). in one year, it has infected over 500,000 people (suspected and confirmed cases) in 40 countries and territories in the americas. along with recent epidemics of dengue (denv) and chikungunya virus (chikv), which are also transmitted by aedes aegypti and ae. albopictus mosquitoes, the emergence of zikv s ...201728182667
severe chikungunya infection in northern mozambique: a case report.although chikungunya virus has rapidly expanded to several countries in sub-saharan africa, little attention has been paid to its control and management. until recently, chikungunya has been regarded as a benign and self-limiting disease. in this report we describe the first case of severe chikungunya disease in an adult patient in pemba, mozambique.201728179029
transcriptome analysis of aedes aegypti in response to mono-infections and co-infections of dengue virus-2 and chikungunya virus.chikungunya virus (chikv) and dengue virus (denv) spread via the bite of infected aedes mosquitoes. both these viruses exist as co-infections in the host as well as the vector and are known to exploit their cellular machinery for their replication. while there are studies reporting the changes in aedes transcriptome when infected with denv and chikv individually, the effect both these viruses have on the mosquitoes when present as co-infections is not clearly understood. in the present study, we ...201728161634
chikungunya virus: an update on the biology and pathogenesis of this emerging pathogen.re-emergence of chikungunya virus, a mosquito-transmitted pathogen, is of serious public health concern. in the past 15 years, after decades of infrequent, sporadic outbreaks, the virus has caused major epidemic outbreaks in africa, asia, the indian ocean, and more recently the caribbean and the americas. chikungunya virus is mainly transmitted by aedes aegypti mosquitoes in tropical and subtropical regions, but the potential exists for further spread because of genetic adaptation of the virus t ...201728159534
engineered aedes aegypti jak/stat pathway-mediated immunity to dengue virus.we have developed genetically modified ae. aegypti mosquitoes that activate the conserved antiviral jak/stat pathway in the fat body tissue, by overexpressing either the receptor dome or the janus kinase hop by the blood feeding-induced vitellogenin (vg) promoter. transgene expression inhibits infection with several dengue virus (denv) serotypes in the midgut as well as systemically and in the salivary glands. the impact of the transgenes dome and hop on mosquito longevity was minimal, but it re ...201728081143
identification and initial characterization of matrix metalloproteinases in the yellow fever mosquito, aedes aegypti.aedes aegypti is a major vector for arboviruses such as dengue, chikungunya and zika viruses. during acquisition of a viremic bloodmeal, an arbovirus infects mosquito midgut cells before disseminating to secondary tissues, including the salivary glands. once virus is released into the salivary ducts it can be transmitted to another vertebrate host. the midgut is surrounded by a basal lamina (bl) in the extracellular matrix, consisting of a proteinaceous mesh composed of collagen iv and laminin. ...201728054419
a large scale biorational approach using bacillus thuringiensis israeliensis (strain am65-52) for managing aedes aegypti populations to prevent dengue, chikungunya and zika transmission.aedes aegypti is a container-inhabiting mosquito and a vector of dengue, chikungunya, and zika viruses. in 2009 several cases of autochthonous dengue transmission were reported in key west, florida, usa prompting a comprehensive response to control a. aegypti. in key west, larvae of this mosquito develop in containers around human habitations which can be numerous and labor intensive to find and treat. aerial applications of larvicide covering large areas in a short time can be an efficient and ...201728199323
levels of insecticide resistance to deltamethrin, malathion, and temephos, and associated mechanisms in aedes aegypti mosquitoes from the guadeloupe and saint martin islands (french west indies).in the guadeloupe and saint martin islands, aedes aegypti mosquitoes are the only recognized vectors of dengue, chikungunya, and zika viruses. for around 40 years, malathion was used as a mosquito adulticide and temephos as a larvicide. since the european union banned the use of these two insecticide molecules in the first decade of the 21st century, deltamethrin and bacillus thuringiensis var. israelensis are the remaining adulticide and larvicide, respectively, used in guadeloupe. in order to ...201728187780
response of three cyprinid fish species to the scavenger deterrent factor produced by the mutualistic bacteria associated with entomopathogenic nematodes.the symbiotic bacteria, photorhabdus and xenorhabdus associated with entomopathogenic nematodes (epns) in the genera heterorhabditis and steinernema, respectively, produce a compound(s) called the scavenging deterrent factor (sdf). sdf deters a number of terrestrial insect scavengers and predators and one bird species from feeding on host insects killed by the nematode-bacterium complex but has not been tested against aquatic vertebrates. moreover, the heterorhabditis-photorhabdus association is ...201727908637
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