Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| mutagenesis of two surface-exposed loops of the bacillus thuringiensis cryic delta-endotoxin affects insecticidal specificity. | site-directed mutagenesis was used to determine the role of two surface-exposed loops (gly-317-phe-320 and gln-374-pro-377) in the insecticidal specificity of the bacillus thuringiensis cryic delta-endotoxin. mutant toxins were generated by pcr using degenerate oligonucleotide primers, and expressed in escherichia coli. more than 50 mutant toxins were screened for toxicity to the lepidopteran spodoptera frugiperda sf9 cell line using an in vitro lawn assay. a panel of these mutant toxins, which ... | 1994 | 8093015 |
| filaria vector competence of some anopheles species. | the filaria vector competence of anopheles stephensi was compared with brugia-susceptible aedes aegypti liverpool strain, an. gambiae badagry lagos strain and an. dirus perlis malaysia strain. an. stephensi ingested more brugia pahangi microfilariae, had the highest infectivity rate and yielded more infective mosquitoes than the other two anopheline species. the overall vector competence of an. stephensi was 0.13 times that of ae. aegypti, 0.62 times that of an. gambiae and 2.17 times that of an ... | 1994 | 8066378 |
| mosquito repellency & toxicity of isomeric n, n-diethyltolylacetamides. | three isomers of n, n-diethyltolylacetamides were synthesized and evaluated against aedes aegypti and culex quinquefasciatus for repellency and with mice for ld50, as an indicator of mammalian toxicity. of these, n, n-diethyl-p-tolylacetamide (depta) showed comparatively better repellency against c. quinquefasciatus. all the compounds showed higher repellency against c. quinquefasciatus than ae. aegypti. in addition, n, n-diethyl-m-tolylacetamide (demta) was found to be least toxic to mice. | 1994 | 8063347 |
| use of randomly amplified polymorphic dna amplified by polymerase chain reaction markers to estimate the number of aedes aegypti families at oviposition sites in san juan, puerto rico. | we report the application of a molecular genetic technique to estimate the number of full-sibling families of aedes aegypti contained in oviposition traps. randomly amplified polymorphic dna amplified by the polymerase chain reaction markers were used to estimate the numbers and sizes of families in traps at field locations in san juan, puerto rico. forty-nine presumptive loci were amplified with five primers in a total of 813 individuals from 26 sites. the average family size was 10.95, but the ... | 1994 | 8059920 |
| triglyceride-rich lipophorin in aedes aegypti (diptera: culicidae). | a lipoprotein was isolated from aedes aegypti (l.) larvae. this lipoprotein resembled the typical lipoprotein responsible for lipid transport in insect hemolymph, also called lipophorin. the mosquito lipophorin had a hydrated density of 1.113 g/ml and contained 49% lipid and 3.2% carbohydrate. in contrast to all other insect species studied thus far, the major lipid component of the mosquito lipophorin was triacylglycerol. the native lipophorin had an apparent molecular weight of 480 kd and cont ... | 1994 | 8057318 |
| isolation and identification of three leucokinins from the mosquito aedes aegypti. | three leucokinins were isolated from the mosquito aedes aegypti. the amino acid sequences of the aedes leukokinins 1, 2 and 3 have been determined to be asn-ser-lys-tyr-val-ser-lys-gln-lys-phe-tyr-ser-trp-gly-amide, asn-pro-phe-his-ala-trp-gly-amide and asn-asn-pro-asn-val-phe-tyr-pro-trp-gly-amide, respectively. | 1994 | 8048942 |
| effect of ambrosia maritima on anopheles stephensi and aedes aegypti. | the toxicity of the molluscicidal plant ambrosia maritima l. was evaluated against anopheles stephensi and aedes aegypti. in the larvicidal assays a negligible mortality was observed in both species after application of the dried leaves in the water at concentrations up to 2000 mg/l. when the powdered leaves were applied at the surface of the water, however, up to 38% of the larvae of a. stephensi were killed at 2000 mg/l. virtually no inhibitory effect on the larval growth of both species was n ... | 1994 | 8046947 |
| toxicity of newly synthesized pyrethroids against the yellow fever mosquito larvae, aedes aegypti (diptera: culicidae). | synthesis of p-substituted benzyl esters of (+/-) cis 2,2-dimethyl-3-(2,2-dichlorovinyl)cyclopropane-carboxylic acid has been reported. (+/-) cis dichlorovinyl acid esters of p-substituted phenols acquired insecticidal activity after introduction of methylene group. the insecticidal activity shown against mosquito larvae a. aegypti by compounds having electron withdrawing groups such as -no2, -cn was substantially higher than that of the compounds having electron-donating groups such as -ch3, -o ... | 1994 | 8045613 |
| frequent occurrence of ige and igg4 antibodies against saliva of aedes communis and aedes aegypti mosquitoes in children. | we examined the prevalence of ige and igg4 class antibodies to the saliva of aedes communis and aedes aegypti mosquitoes in the sera of three groups of exposed children using a sensitive immunoblot method. the frequencies of ige antibodies to the major 36-kd a. communis and a. aegypti saliva antigens ranged from 82 to 90% in the 20 finnish, 17 kenyan, and 20 mexican children. the corresponding igg4 antibody frequencies were 85, 41, and 20%, respectively. the nonexposed 20 icelandic children did ... | 1994 | 8038615 |
| purification of an insect defensin from the mosquito, aedes aegypti. | using a new, sensitive assay of bacterial growth inhibition, inducible antibacterial activity has been identified in the haemolymph of the mosquito, aedes aegypti following inoculation with bacteria or with microfilariae of the filarial nematode brugia pahangi, but not after inoculation with sterile culture medium. a lower level of antibacterial activity has also been observed in untreated individual mosquitoes. following bacterial inoculation, a basic, inducible antibacterial peptide has been d ... | 1994 | 8025559 |
| community-based integrated control of aedes aegypti: a brief overview of current programs. | dengue viruses are maintained in endemic transmission cycles in tropical urban areas where epidemics periodically occur. until about 30 years ago, there were long intervals (10-40 years) between epidemics but they are now occurring in many areas at 3-5-year intervals. these epidemics are most likely caused by virus strains with different epidemic potential. accompanying this increased frequency in epidemic activity has been a change in the disease pattern with cases of the severe form of dengue ... | 1994 | 8024084 |
| protozoan-enhanced toxicity of bacillus thuringiensis var. israelensis delta-endotoxin against aedes aegypti larvae. | the toxicity of bacillus thuringiensis var. israelensis (bti) in mosquito larvae was enhanced by encapsulation in the protozoan tetrahymena pyriformis. aedes aegypti larvae which fed on t. pyriformis loaded with bti died about three times faster than when fed on the same concentrations of bti alone due to ingestion of higher toxin concentrations, reflected by shorter death times of exposed populations. the best larvicidal activities were achieved at ratios of cell/spore numbers in the range of 1 ... | 1994 | 8021522 |
| studies on the inheritance of repellent tolerances in aedes aegypti. | two laboratory strains and 6 inbred strains of aedes aegypti were tested against deet, ethyl hexanediol, dimethyl phthalate, and indalone. reciprocal crosses and backcrosses of 2 inbred strains were tested against deet only. results obtained were compatible with a quantitative genetic model in which the effects of the factors involved were multiplicative. certain inbred strains differed significantly from cognate laboratory and/or inbred strains in tolerance to one or more test materials. herita ... | 1994 | 8014634 |
| reproductive isolation between florida strains of aedes aegypti and aedes albopictus. | the dynamics of heterospecific and conspecific mating between florida strains of aedes aegypti and aedes albopictus was examined. in nonchoice experiments where conspecific males were not available, dissection of the spermathecae showed that heterospecific insemination was an infrequent event for both species combinations (10.6% for ae. albopictus with ae. aegypti males, 3.6% for the reciprocal cross). few eggs were produced from heterospecific crosses and all were nonviable. frequency of hetero ... | 1994 | 8014633 |
| comparison of bi-directional fay, omni-directional, cdc, and duplex cone traps for sampling adult aedes albopictus and aedes aegypti in north florida. | we compared the number of aedes albopictus and aedes aegypti females collected in cdc, duplex cone, bi-directional fay, and new omni-directional traps in a series of trials in northern florida during 1992 and 1993. bi-directional fay and omni-directional traps collected significantly more ae. albopictus females than did the other traps tested. the bi-directional fay trap collected significantly more ae. aegypti females than did any other trap. the results of these studies indicate that these tra ... | 1994 | 8014632 |
| use of the "cdc backpack aspirator" for surveillance of aedes aegypti in san juan, puerto rico. | we constructed a battery-powered backpack aspirator to collect adult aedes aegypti mosquitoes. this simple, easily constructed aspirator facilitates the indoor collection of this important vector species. the collections made with the aspirator provide useful information about the biology and behavior of ae. aegypti that can be used in education and vector control programs and in the evaluation of ultra-low volume insecticide spray programs directed against this species. the cost for constructio ... | 1994 | 8014622 |
| meiosis and chromosomal effects of thio-tepa in the ovarian cells of aedes aegypti. | the development of meiosis during oogenesis in the normal, and 0.1% thio-tepa (triethylene thiophosphoramide) fed female of aedes aegypti was studied. chromosome preparations were made from adults 24 h after a blood meal. the frequency of various kinds of chromosomal abnormalities was analysed. | 1994 | 8001399 |
| evaluation of organophosphorus and synthetic pyrethroid insecticides against six vector mosquitoe species. | three organophosphorus compounds- malathion, folithion and temephos- and two synthetic pyrethroids- alphamethrin and deltamethrin- were used for monitoring the susceptibility status of larvae and adults of six vector mosquito species: culex quinquefasciatus (filariasis) and aedes albopictus (dengue) (both laboratory and field strains); laboratory strains of aedes aegypti (dengue), anopheles stephensi and anopheles culicifacies (malaria), and culex tritaeniorhynchus (japanese encephalitis) in ind ... | 1993 | 7997809 |
| location of a lepidopteran specificity region in insecticidal crystal protein cryiia from bacillus thuringiensis. | the bacillus thuringiensis insecticidal crystal protein cryiia has both high mosquito activity and gypsy moth activity; in contrast cryiib, which is 87% homologous, displays no mosquito activity and has a three-fold lower gypsy moth activity. the regions responsible for specificity against gypsy moth (lymantria dispar) and mosquito (aedes aegypti) larvae were located by introducing mlui and xhoi sites into homologous positions within the putative domain ii of both cryiia and cryiib genes, which ... | 1994 | 7997170 |
| chromosomal mapping of two loci affecting filarial worm susceptibility in aedes aegypti. | two quantitative trait loci (qtl) affecting susceptibility of the mosquito aedes aegypti to the filarial worm parasite brugia malayi were identified using restriction fragment length polymorphism (rflp) markers. the first locus, fsb[1,lf178], resides within a 10 cm interval on chromosome 1 and exhibits a recessive effect with respect to susceptibility. the second locus, fsb[2,lf98], resides within a 9 cm interval on chromosome 2 and exhibits an additive effect on susceptibility. significant epis ... | 1994 | 7987523 |
| differential modulation of murine cellular immune responses by salivary gland extract of aedes aegypti. | the ability of salivary gland extract (sge) of aedes aegypti to modulate cellular immune responses was investigated in a mouse model. cytokine production was induced in naive and antigen-primed murine (balb/c) spleen cells in vitro by stimulation with the t cell mitogen concanavalin a or the t cell-dependent antigen ovalbumin (ova), respectively. inclusion of ae. aegypti sge in in vitro culture with naive cells caused significant suppression of the cytokines interleukin-2 (il-2) and interferon g ... | 1994 | 7985763 |
| elisa for detecting dengue and japanese encephalitis viral antigen in mosquitoes. | an indirect elisa has been developed for detecting two viruses in triturated, experimentally infected mosquitoes: dengue (den) in aedes aegypti (l.) and ae. albopictus (skuse) and japanese encephalitis (je) viral antigen in culex tritaeniorhynchus giles. den antigen from four strains of the virus, representing each of the four serotypes, and je antigen were captured with a polyclonal anti-flavivirus igg and detected with a monoclonal antibody (4g2) that reacts with all flaviviruses. minimum vira ... | 1994 | 7979627 |
| [seasonal variations in aedes aegypti populations in monterrey, mexico]. | seasonal variation of the populations of the dengue vector mosquito aedes aegypti, in the city of monterrey, n.l., mexico, showed a bimodal pattern. the first peak is lower and appeared at early june, while the second and higher one was observed in the second week of october. 1,419 female mosquitoes were caught in this study. females with fresh red blood in the stomach were present in all the catches, gravid females (mature eggs in the ovaries) being more abundant than the non-gravid ones. the g ... | 1994 | 7973991 |
| salivary vasodilators of aedes triseriatus and anopheles gambiae (diptera: culicidae). | salivary vasodilators of aedes aegypti (l.) and anopheles albimanus (wiedemann) were characterized previously as a tachykinin peptide and a catechol oxidase/peroxidase activity, respectively. to verify whether these two different vasodilators also were found in other distantly related members of each mosquito genus, we characterized the vasodilators from a. triseriatus and a. gambiae. a. triseriatus salivary gland homogenates produced a reversible, endothelium dependent vasorelaxation of rabbit ... | 1994 | 7966179 |
| measurement of flight tone differences between female aedes aegypti and a. albopictus (diptera: culicidae). | mosquito flight tone was amplified and digitally sampled at 20,000 samples per second (hz). resampling of the resulting sound files at 1,000, 5,000, and 10,000 hz allowed comparison of flight tone frequency distributions for males and females of aedes aegypti (l.) and a. albopictus (skuse). frequency distributions for females of the two species did not overlap at sampling rates of 5,000 hz or higher, whereas considerable overlap was observed at the 1,000 hz sampling rate. males of the two specie ... | 1994 | 7966173 |
| insecticide susceptibility in mosquitoes (diptera: culicidae) from french polynesia. | susceptibility to six organophosphate (op), two pyrethroid (py), and one carbamate (c) insecticides was investigated in culex pipiens quinquefasciatus say, aedes aegypti (l.), and aedes polynesiensis marks larvae from the island of tahiti. cx. p. quinquefasciatus and ae. aegypti were compared with susceptible reference strains treated simultaneously. a low, but significant, resistance to bromophos (4.6x), chlorpyrifos (5.7x), fenthion (2.4x), fenitrothion (5.0x), temephos (4.3x) and permethrin ( ... | 1994 | 7966164 |
| densovirus of aedes aegypti as an expression vector in mosquito cells. | we have constructed an infectious dna clone containing the genome of aedes aegypti densovirus (aednv) in a bacterial plasmid. when this clone was transfected into aedes albopictus c6/36 mosquito cells, the aednv genome rescued from the plasmid and replicated as the wild-type virus. to investigate the cloned virus as an expression vector, the reporter gene encoding beta-galactosidase (beta-gal) was inserted into four large open reading frames (orf) observed in the aednv genome. when these recombi ... | 1994 | 7957754 |
| aedes aegypti: characterization of a hemolymph polypeptide expressed during melanotic encapsulation of filarial worms. | we report on the initial characterization of an 84-kda polypeptide that is differentially expressed in aedes aegypti during melanotic encapsulation immune reactions against filarial worms. [35s]methionine-labeled hemolymph from mosquitoes inoculated with saline, parasites that are melanized, or parasites that are not melanized was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. results show that the level of the 84-kda polypeptide increases considerably in those mosquitoes ... | 1994 | 7957753 |
| microscopic visualization of wuchereria and brugia larval stages in intact cleared mosquitoes. | over the past several decades, epidemiologic data from filarial vectors typically has been obtained by mass dissection or by dissection of individual specimens. the former is quick and easy to do on large numbers of insects but provides no information on the frequency distribution of infection, presence of early developmental stages, or larval location; the latter is labor-intensive and tedious. we describe a new technique that can provide data comparable to those obtained by individual dissecti ... | 1994 | 7943576 |
| inheritance of oral susceptibility of aedes aegypti to chikungunya virus. | a colony of rosy eye mutants of aedes aegypti was established. this strain was refractory to chikungunya virus by oral route of infection when compared with the wild-type parent strain. the refractoriness of this strain seems to be due to a mesentronal barrier, since both the mosquito strains supported the multiplication of virus after intrathoracic inoculation. the rosy eye strain was also found to be refractory to sagiyama virus (alphaviridae: getah virus subtype) when compared with wild-type ... | 1994 | 7943547 |
| mosquito gonotrophic cycle and multiple feeding potential: contrasts between anopheles and aedes (diptera: culicidae). | two aedine and three anopheline species were allowed to blood-feed to repletion, and their host-seeking behavior was measured with an olfactometer at intervals afterward. both aedes aegypti (l.) and ae. albopictus (skuse) were inhibited from subsequently seeking a host during the gonotrophic cycle, but anopheles gambiae giles, an. albimanus wiedemann, and an. freeborni aitken, all showed varying degrees of host-seeking behavior while their eggs matured. continued host-seeking by some anophelines ... | 1994 | 7932610 |
| reproductive fitness and survivorship of aedes aegypti (diptera: culicidae) maintained on blood, with field observations from thailand. | daily survivorship and fecundity of aedes aegypti mosquitoes were monitored for cohorts provided with five nutritional treatments: water, 2% sucrose, blood from a live chicken plus water, blood plus 2% sucrose, and blood alone. the median mortality time (lt50) for these females was 6, 54, 16, 12, and 29 d, respectively. there was no significant difference in the number of eggs laid by females in any of the treatments containing a host. females maintained on blood alone laid as many or more eggs ... | 1994 | 7932609 |
| reinterpretation of the genetics of susceptibility of aedes aegypti to plasmodium gallinaceum. | several studies have demonstrated a genetic basis for variation in susceptibility of aedes aegypti to plasmodium gallinaceum. although 25 yr ago it was reported that p. gallinaceum susceptibility in ae. aegypti is determined primarily by a single autosomal dominant gene, evidence for additional genetic factors has emerged. two sublines, 1 refractory and 1 of intermediate susceptibility to p. gallinaceum, have been selected from the moyo-in-dry strain (moyo) of ae. aegypti. prior to selection, th ... | 1994 | 7931905 |
| biochemical basis of ddt-resistance in aedes aegypti population from a dengue affected area in shahjahanpur city. | entomological studies conducted in jalalnagar, shahjahanpur city, uttar pradesh, india, during an outbreak of dengue in 1992, showed that ae. aegypti mosquitoes were resistant to ddt and had some tolerance to malathion in the adults and the larvae. biochemical analysis suggested that ddt resistance was related to elevated glutathione s-transferase and tolerance to malathion was due to a little increase in esterase activity. crosses of ddt-resistant and susceptible strain suggested that resistanc ... | 1994 | 7927578 |
| host range tests with edhazardia aedis (microsporida: culicosporidae) against northern nearctic mosquitoes. | twelve species of northern nearctic mosquitoes representing five genera (aedes, anopheles, culex, culiseta, and psorphora) were bioassayed for susceptibility to edhazardia aedis, an exotic, heterosporous, microsporidian parasite of the yellow fever mosquito, aedes aegypti. infections were achieved in aedes atropalpus, aedes triseriatus, and aedes vexans following oral ingestion of uninucleate spores obtained from patently infected ae. aegypti larvae. the microsporidium underwent normal vegetativ ... | 1994 | 7914904 |
| efficacy of clostridium bifermentans serovar malaysia on target and nontarget organisms. | clostridium bifermentans serovar malaysia (c.b.m.) is highly toxic to mosquito larvae. in this study, the following aquatic nontarget invertebrates were treated with high c.b.m. concentrations (up to 1,600-fold the toxic concentration for anopheles stephensi) to study their susceptibility towards the bacterial toxin: planorbis planorbis (pulmonata); asellus aquaticus (isopoda); daphnia pulex (cladocera); cloeon dipterum (ephemeroptera); plea leachi (heteroptera); and eristalis sp., chaoborus cry ... | 1994 | 7912261 |
| adult cat flea (siphonaptera: pulicidae) excretion of host blood proteins in relation to larval nutrition. | protein content and ion composition of host blood and feces from blood-fed adult cat fleas, ctenocephalides felis (bouché), were examined. total fecal protein differed slightly from host blood indicating that there was little digestion of host blood by adult fleas. sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels of host blood and flea feces also revealed little digestion, as evidenced by the quantitatively and qualitatively similar protein banding patterns. blood and fecal ion com ... | 1994 | 7910638 |
| a comparative study of leucokinin-immunoreactive neurons in insects. | antisera were raised against leucokinin iv, a member of the leucokinin peptide family. immunohistochemical localization of leucokinin immunoreactivity in the brain of the cockroach nauphoeta cinerea revealed neurosecretory cells in the pars intercerebralis and pars lateralis, several bilateral pairs of interneurons in the protocerebrum, and a group of interneurons in the optic lobe. several immunoreactive interneurons were found in the thoracic ganglia, while the abdominal ganglia contained prom ... | 1994 | 7910521 |
| the suitability of restriction fragment length polymorphism markers for evaluating genetic diversity among and synteny between mosquito species. | restriction fragment length polymorphism (rflp) markers derived from the yellow fever mosquito, aedes aegypti, were used in hybridizations to genomic dna of the following mosquito species: ae. albopictus, ae. togoi, armigeres subalbatus, culex pipiens, and anopheles gambiae. interspecific hybridization with ae. aegypti probes varied from 50% (an. gambiae) to 100% (ae. albopictus) under high stringency conditions. we demonstrated the usefulness of using rflp profiles to examine genetic diversity ... | 1994 | 7909414 |
| experimental infection of aedes aegypti mosquitoes, suckling mice, and rats with four mosquito spiroplasmas. | four mosquito spiroplasmas, spiroplasma culicicola, spiroplasma sabaudiense, spiroplasma taiwanense, and an isolate designated cuas-1 were nonpathogenic for suckling mice and suckling rats inoculated either intracerebrally or intraperitoneally. s. culicicola persisted as long as 14 days in the brain of intracerebrally inoculated mice but did not replicate. the three other spiroplasmas were not reisolated from inoculated rats or mice. s. culicicola replicated in vitro at 37 degrees c in m1d media ... | 1994 | 7906284 |
| mosquito host range and specificity of edhazardia aedis (microspora: culicosporidae). | edhazardia aedis was transmitted horizontally to its natural host, aedes aegypti, and to 6 alternate hosts: ae. albopictus, ae. triseriatus, ae. taeniorhynchus, anopheles quadrimaculatus, orthopodomyia signifera and toxorhynchites rutilus rutilus. the microsporidium produced both binucleate and uninucleate spores in all susceptible hosts. transovarial transmission, however, was only successful in ae. aegypti. therefore, while e. aedis can infect a variety of mosquito species from diverse genera, ... | 1993 | 7902414 |
| 5-hydroxytryptamine in the salivary glands of adult female aedes aegypti and its role in regulation of salivation. | a dense plexus of axons, immunoreactive to antisera against 5-hydroxytryptamine (5-ht, serotonin) and surrounding the proximal medial lobe of the salivary gland of adult female aedes aegypti mosquitoes, was demonstrated by means of whole-mount fluorescence immunocytochemistry. this innervation originates in the stomatogastric nervous system. 5-ht-immunoreactive innervation is absent in male salivary glands, suggesting that 5-ht is involved in blood-feeding. furthermore, female mosquitoes treated ... | 1995 | 7891033 |
| time to death from starvation and compulsive killing by the larvae of toxorhynchites splendens (diptera: culicidae). | time to death from starvation and compulsive killing without eating of the prey by larvae of toxorhynchites splendens were studied in the laboratory. the first and second instars survived without food for 3 days while third and fourth instars survived for 7.8 and 14 days, respectively. when the corresponding instars of aedes aegypti, anopheles stephensi or culex quinquefasciatus were offered, the number of prey killed but not eaten ranged from 0 to 15 per 40 prey larvae. compulsive killing of ae ... | 1994 | 7887340 |
| effect of some new insect growth regulators on metamorphosis & reproduction of aedes aegypti. | fourth instar larvae and pupae of ae. aegypti were treated with four most active insect growth regulators from a new series of mixed alkyl and aryl diethers based on geraniol. considerable reduction in fecundity and fertility of adults was obtained. treatment of pupae or pharate adults did not affect adult emergence. topical treatment of adult females caused great reduction in fertility and fecundity in older as compared to younger females. in addition to the effects on reproduction, adult survi ... | 1995 | 7883278 |
| inhibition of mouse parenchymal cells proliferation by mosquito larvae extract. | as it is known, some cell populations of mosquito aedes aegypti larvae do not proliferate. this fact suggests the existence of some mitosis inhibiting factor. we have assayed the effect of mosquito larvae crude extract on 85 c3hs young male mice (25 +/- 1 days old) and the effect of the dialyzed fraction of the same extract on 47 partially hepatectomized c3hs adult male mice. the injection was performed at 16:00 hour (before the raising of the dnas curve) and the controls were made at 08:00/16, ... | 1994 | 7873990 |
| short report: dispersal of aedes aegypti in an urban area after blood feeding as demonstrated by rubidium-marked eggs. | strategies for the control of aedes aegypti during urban outbreaks of dengue or yellow fever assume that this species has a maximum flight range of 50-100 meters. because ae. aegypti distributes its eggs among several oviposition sites, we postulated that dispersal is driven by the search for oviposition sites, so an ovipositing female may have to fly much further than 50-100 meters to lay all of her eggs. we developed a method for marking ae. aegypti eggs with a rare alkali metal (rubidium) and ... | 1995 | 7872449 |
| growth-restricted dengue virus mutants containing deletions in the 5' noncoding region of the rna genome. | the dengue type 4 virus (den4) rna genome contains a 101-nt 5' noncoding (nc) sequence which is predicted to form a stable secondary structure. den4 cdna from which infectious rna can be transcribed was used to engineer deletions in the 5' nc region for functional analysis of rna structure and for isolation of den4 mutants that could be evaluated as candidates for use in a live attenuated vaccine. eleven distinct deletions in the region of the den4 genome between nts 18 and 98 were constructed; ... | 1995 | 7871753 |
| estimates of population size, dispersal, and longevity of domestic aedes aegypti aegypti (diptera: culicidae) by mark-release-recapture in the village of shauri moyo in eastern kenya. | estimates of the absolute size of a domestic population of aedes aegypti aegypti (l.) were made in the rabai area of kenya, based on a single release, followed by either single or repeated recaptures. from single recapture within 24 h of release, the size of the female ae. a. aegypti population in the shauri moyo village was estimated by the lincoln index to be 365. using a single release and repeated recaptures, population size was estimated by jackson's positive method to be 337. depletion of ... | 1995 | 7869339 |
| comparative repellent properties of certain chemicals against mosquitoes, house flies and cockroaches using modified techniques. | several terpenoids were assessed for their repellent/toxic properties against mosquitoes (aedes aegypti), house flies (musca domestica) and cockroaches (periplaneta americana). impregnated wide mesh netting was used in the case of the dipterans, while treated filtered paper was employed for the bioassays with cockroaches. persistence of the repellent chemicals was studied. doses ranged from 5-20 gm/m2 for the dipterans and 25-100 mg per 4 x 4 cm filter paper for the cockroaches. dimethyl phthala ... | 1994 | 7868839 |
| synergism of mosquitocidal toxicity between cyta and cryivd proteins using inclusions produced from cloned genes of bacillus thuringiensis. | the toxicity to mosquito larvae of the parasporal body produced by bacillus thuringiensis subsp. israelensis and the pg-14 isolate of b. thuringiensis subsp. morrisoni is at least 20-fold greater than any of the four mosquitocidal proteins of which it is composed (cyta, cryiva, b, and d). this high toxicity is postulated to be due to synergistic interactions among parasporal proteins. however, this remains controversial because values reported for the specific toxicity of individual proteins, es ... | 1994 | 7854129 |
| the salivary gland-specific apyrase of the mosquito aedes aegypti is a member of the 5'-nucleotidase family. | the saliva of hematophagous insects contains a variety of pharmacologically active substances that counteract the normal hemostatic response to injury in vertebrate hosts. the yellow-fever mosquito, aedes aegypti, secretes an apyrase that inhibits adp-dependent platelet aggregation. apyrase was purified as an active enzyme from adult female salivary glands and subjected to tryptic digestion, and the resulting peptides were sequenced. the amino acid sequences obtained match the conceptual transla ... | 0 | 7846038 |
| concomitant malaria (plasmodium gallinaceum) and filaria (brugia pahangi) infections in aedes aegypti: effect on parasite development. | mixed infections with malarial (plasmodium gallinaceum) and filarial (brugia pahangi) parasites were carried out in 8 trials with filaria susceptible (refm) and filaria refractory (rep-rr) aedes aegypti strains. a secondary infection with b. pahangi microfilariae (mff) by intrathoracic inoculation, reduced the development rate of a pre-existing p. gallinaceum infection. the level of reduction ranged from 9.5 to 49% in refm and from 50 to 90% in rep-rr. an immune response against oocysts was seen ... | 1995 | 7845706 |
| surveillance and control of aedes aegypti in epidemic areas of taiwan. | aedes aegypti is the main, if not the only, vector of dengue fever in taiwan. the dengue epidemics that have occurred in taiwan correlate with the distribution of aedes aegypti which is limited to south of the tropic of cancer. during the 1987 outbreak of dengue fever in taiwan, the average larval density for the months july-december in the five cities and counties of southern taiwan was 2,284 larvae per 100 households. after control measures were taken, the average annual larval density in the ... | 1994 | 7844856 |
| ecology and control of dengue vector mosquitoes in taiwan. | due to rapid urbanization, industrialization and social changes in recent years, the use of packing materials and tires has dramatically increased in the taiwan area. what is more is that some parts of southern taiwan are short of water resources and water preservation with huge containers becomes part of custom in those areas. storage water containers, waste vessels and tires are good habitats for aedes. meanwhile, some persons traveling to dengue endemic countries bring the dengue disease back ... | 1994 | 7844855 |
| intersectoral approaches to dengue vector control. | medical entomology in the context of urban vector control, especially for dengue, can be likened to the tail of a dog. vertically structured aedes aegypti campaigns such as run by gorgas and soper earlier this century relied on sufficient legislative backing for vector control to ensure that the tail was capable of wagging the dog. under these conditions, especially where individual rights do not intrude, vertical programs will be successful. the global expansion of dengue, dengue hemorrhagic fe ... | 1994 | 7844851 |
| control of dengue vectors in singapore. | singapore has a well-established system for the surveillance, research and control of aedes aegypti and aedes albopictus. control of these vectors comes under the jurisdiction of the environmental public health division of the ministry of the environment. the strategy for aedes control is an integrated approach incorporating case detection, source reduction, health education and law enforcement. this is done through the quarantine & epidemiology department (dengue surveillance and research), vec ... | 1994 | 7844848 |
| dengue vector control in the urban environment of taiwan. | an epidemic of dengue occurred in southern taiwan in 1987, and by the end of 1988 there were 10,420 reported cases, of which 60.5% were from kaohsiung municipality. the urbanization and industrialization of the municipality have resulted in a vast increase in the breeding habitats of the dengue vector, aedes aegypti. in the early stage of the epidemic, only space spraying with insecticides was undertaken, but the aedes density gradually increased and reached its peak in the third quarter of 1988 ... | 1994 | 7844847 |
| dengue in the health transition. | the "health transition" describes the medical consequences which accompany the demographic transition and development. in many asian countries, as the infectious diseases of infancy decline, such as diarrhea, acute respiratory disease, measles and malaria, so too, do infant mortality rates. as a consequence of falling infant mortality rates and declines in fertility, the age pyramid has become more rectangular. no longer is nearly half of the population under the age of 15 years. diseases of adu ... | 1994 | 7844846 |
| education and employment of medical entomologists in aedes aegypti control programmes. | most dengue/dhf endemic countries have facilities for the training of entomologists. however, experience has shown that many of the graduates of such courses remain in the area of research and relatively few are employed in aedes aegypti vector-control programmes. few university courses in medical entomology provide specific training in vector control including such subjects as the organization and administration of vector control programmes and vector-control methods; chemical, biological and e ... | 1994 | 7844845 |
| dengue vector control: present status and future prospects. | dengue fever (df) and dengue haemorrhagic fever (dhf) have been the most common urban diseases in southeast asia since the 1950s. more recently, the diseases have spread to central and south america and are now considered as worldwide diseases. both aedes aegypti and aedes albopictus are involved in the transmission of df/dhf in southeast asian region. the paper discusses the present status and future prospects of aedes control with reference to the malaysian experience. vector control approache ... | 1994 | 7844836 |
| larvicidal microcystin toxins of cyanobacteria affect midgut epithelial cells of aedes aegypti mosquitoes. | 1994 | 7841498 | |
| comparative study of hemolymph phenoloxidase activity in aedes aegypti and anopheles quadrimaculatus and its role in encapsulation of brugia malayi microfilariae. | hemolymph phenoloxidase activity of sugar-fed and blood-fed females of anopheles quadrimaculatus and aedes aegypti showed similar characteristics. phenoloxidase was present as an inactive proenzyme in both mosquito species and was partially activated during collection of the hemolymph. in both mosquito species, phenoloxidase activity was modulated by different buffers and activated phenoloxidase did not need ca2+. enzymatic activity was higher in the hemocytes than in the plasma in both mosquito ... | 1994 | 7828033 |
| egg chorion tanning in aedes aegypti mosquito. | the biochemical pathway of egg chorion tanning in the mosquito, aedes aegypti, is described and compared with chorion protein crosslinking in drosophila and silkmoths and the biochemical pathways of cuticular tanning in insects. phenol oxidase, dopa decarboxylase and tyrosine are critical components involved in egg chorion tanning in a. aegypti. tanning of the mosquito egg chorion is initiated following activation of phenol oxidase, which then catalyzes the hydroxylation of tyrosine to dopa and ... | 1994 | 7828027 |
| an efficient and easy method of infection of mosquito larvae from virus-contaminated cell cultures. | a new method for efficient infection of aedes aegypti larvae by the aedes albopictus densovirus, aapv is described. it consists of placing first or third instar larvae in culture flasks containing a chronically infected mosquito cell line. after 24 or 48 h of exposure to the contaminated culture, the larvae acquired the virus by feeding on infected cells. using this technique, up to 95% of first instar ae. aegypti larvae were found infected by the aapv. | 1994 | 7822456 |
| [current situation of yellow fever in latin america]. | yellow fever endemicity is currently stabilized in south america: an average of 115 cases has officially been notified each year since 25 years (between 12 and 304 annual cases). these figures are underevaluated but no epidemic has been observed since 1983. only sporadic or limited grouped cases have been reported. all declared cases correspond to people who have been infected within sylvatic areas, mainly adult forestmen. within the last years, the majority of cases have occurred in peru and bo ... | 1993 | 7819801 |
| [dengue epidemic in new caledonia (1989). environmental factors and prevention]. | an extensive dengue epidemic was reported in new caledonia en 1989. environmental factors (temperature and rainfalls) directly controlled the mosquitos population at the end of 1988. the introduction of a new dengue virus, transmitted by aedes aegypti, was responsible for an epidemic that occurred in january-april 1989 and lasted until july 1989. the estimated number of cases was 25,000 and the attack rate was close to 120 per thousand inhabitants for a six month-period. the epidemic involved ma ... | 1993 | 7819798 |
| salivarectomy effects on the ingestion of brugia pahangi (nematoda: filarioidea) microfilariae by susceptible and refractory strains of aedes aegypti (diptera: culicidae). | ingestion of brugia pahangi buckley & edeson microfilariae by susceptible (liverpool) and refractory (bora-bora) strains of salivary duct-severed aedes aegypti (l.) was investigated. the transection at either the salivary common duct or the two lateral ducts of mosquitoes did not affect the mean number of microfilariae nor the mean amount of blood ingested per mosquito. these results indicated that mosquito salivary secretion did not have a chemotropic effect on microfilariae. | 1994 | 7815410 |
| toxicity of extracts from three tagetes against adults and larvae of yellow fever mosquito and anopheles stephensi (diptera: culicidae). | whole-plant soxhlet extractions for the three tagetes species showed that t. minuta had the greatest biocidal effect on the larvae and adults of aedes aegypti (l.) and anopheles stephensi liston. bioassays of simultaneous steam distillation extractions of the various parts of t. minuta found extracts from the flowers provided ld90s of 4 and 8 ppm against the larvae and 0.4 and 0.45% against the adults of a. aegypti and a. stephensi, respectively. further research on t. minuta floral extracts as ... | 1994 | 7815394 |
| a device for monitoring and control of mosquitoes by behaviour manipulation. | a trap was designed and fabricated for capturing mosquito larvae based on their behavioural responses to food and light. the larvae upon entering the trap died ultimately due to asphyxiation. maximum success was achieved with aedes aegypti larvae in lesser water volumes. the usefulness of the device for studying the response of mosquito larvae and aquatic organisms to chemicals, baits, light, various stimuli and possible pest/vector monitoring and management in aquatic eco-system is discussed. | 1994 | 7814048 |
| mosquito transposable elements. | most of the transposons so far characterized from mosquito genomes are retroelements which seem to be distributed worldwide. the juan transposons constitute a family of non-ltr retroelements, or line-retroposons, which are dispersed in the genomes of several mosquito species. three different juan subfamilies have been characterized, each being amplified in the genomes of many strains, if not all, of a given mosquito species. these subfamilies have been designated respectively juan-c in culex pip ... | 1994 | 7813911 |
| the effect of neemazal on aedes aegypti (diptera: culicidae). | the effect of neemazal (trifolio-m gmbh, lahnau), a neem seed kernel extract, on larvae of aedes aegypti was tested. second, third and fourth instar larvae were reared in water containing different concentrations of neemazal. mortality rates and the stage in which mortality occurred were recorded. the surviving larvae were left in the treated water until adult emergence and the number of eggs laid by the emerged females was recorded in order to evaluate possible fecundity-reducing effect of subl ... | 1994 | 7812312 |
| infection, dissemination, transmission, and biological attributes of dengue-2 pdk53 candidate vaccine virus after oral infection in aedes aegypti. | the capacity for oral infection, dissemination, and transmission of the dengue-2 candidate vaccine virus den-2 pdk53 and an isolate from a vaccinate individual, den-2 ia8, were compared with the parent strain den-2 16681. capacity for oral infection and dissemination to the brain and salivary gland tissues were significantly lower in the first two than in the parent strain (p < 0.001). replication was more than 100 times higher for the parent strain when compared with the dengue-2 candidate vacc ... | 1994 | 7810824 |
| a convenient mosquito membrane feeding system. | a convenient, electronically controlled, in situ, membrane feeding system is described. evaluation of the system for feeding single pair-mated aedes aegypti with defibrinated, refrigerated pig blood indicated no significant difference from mouse-fed controls in rat of egg maturation, fecundity, or pupal yield. the feeding system is also suitable for use with substitute protein meals. | 1994 | 7807091 |
| seasonality, prevalence and pathogenicity of the gregarine ascogregarina taiwanensis (apicomplexa: lecudinidae) in mosquitoes from florida. | aedes albopictus larvae collected in gainesville, fl, were infected with the gregarine ascogregarina taiwanensis. natural prevalence varied from 68 to 100%. eight mosquito species were tested in the laboratory for susceptibility to a. taiwanensis isolated from field-collected ae. albopictus. aedes aegypti, aedes albopictus, and aedes taeniorhynchus became 100% infected in the larval stage, whereas aedes triseriatus was less susceptible; culex quinquefasciatus, culex nigripalpus, culex territans, ... | 1994 | 7807086 |
| emergency control of aedes aegypti in the dominican republic using the scorpion 20 ulv forced-air generator. | in an effort to develop a more effective measure for use in emergency control of the dengue vector aedes aegypti. applications of a combination of a larvicide (bacillus thuringiensis israelensis [b.t.i.]) and an adulticide (permethrin) were made using a truck-mounted forced-air generator (scorpion 20) and evaluated in the dominican republic. this method has the potential to simultaneously control adults and larvae. in bioassay cages placed in household water containers at the time of application ... | 1994 | 7807084 |
| aedes albopictus distribution, abundance, and colonization in lee county, florida, and its effect on aedes aegypti. | in 1992 the known southern limit of aedes albopictus in florida was in lee county. through oviposition surveillance, the distribution of ae. albopictus was determined, and its frequency relative to aedes aegypti and colonization pattern of areas previously occupied by ae. aegypti were examined in lee county. the data collected in the first year of surveillance demonstrate the ability of ae. albopictus to rapidly and preferentially colonize large expanses of rural southwest florida. urban and sub ... | 1994 | 7807083 |
| quantitative sampling of immature aedes aegypti in metal drums using sweep net and dipping methods. | the behavior of aquatic stages of aedes aegypti was evaluated in 200-liter metal drums to develop improved surveillance. when known numbers of larvae recovered with a rectangular net and with a standard dipper were compared, all stages except 1st instar were most numerous in the top 1/3 of the drum. water temperature was the only significant variable affecting the vertical distribution of 4th-instar larvae; light intensity and ph were nonsignificant factors. rust in the water was also thought to ... | 1994 | 7807082 |
| utilization of bloodfed females of aedes aegypti as a vehicle for the transfer of the insect growth regulator pyriproxyfen to larval habitats. | bloodfed female aedes aegypti were exposed to a surface treated with pyriproxyfen at 1.0 g m2 for 30 min and then allowed to lay eggs in cups of water containing 4th-instar larvae. adult emergence from the immatures was highly inhibited, and transmission of pyriproxyfen from the females to the water was revealed. the transfer of the chemical to the water decreased with time before the blood meal. chemical analysis for pyriproxyfen on the exoskeleton of treated females demonstrated the rapid disa ... | 1994 | 7807075 |
| aquatic safety of lagenidium giganteum: effects on freshwater fish and invertebrates. | eleven freshwater species were exposed to a zoosporeproducing fungus, lagenidium giganteum, with the goal of determining species sensitivity with standard and new test procedures. the tests included standard, 4-day acute exposures of cladocerans (ceriodaphnia dubia, daphnia pulex, and d. magna) and the fathead minnow (pimephales promelas). standard 7-day chronic exposures of c. dubia and a 7-day embryo-larval exposure of p. promelas were also conducted. new, 4-day acute, methods were developed f ... | 1994 | 7806894 |
| culekinin depolarizing peptide: a mosquito leucokinin-like peptide that influences insect malpighian tubule ion transport. | a peptide termed culekinin depolarizing peptide (cdp) was isolated from approximately 1.2 million mosquitos (94% culex salinarius). the peptide was isolated on the basis of a rapid myotropic assay that utilized a hindgut preparation from leucophaea maderae and a transepithelial voltage assay that used mosquito malpighian tubules from aedes aegypti. a 15% trifluoroacetic acid extraction from the mosquitos, two solid phase extraction steps, and six hplc steps resulted in the isolation of 9.7 nmol ... | 1994 | 7800855 |
| restriction fragment length polymorphism mapping of quantitative trait loci for malaria parasite susceptibility in the mosquito aedes aegypti. | susceptibility of the mosquito aedes aegypti to the malarial parasite plasmodium gallinaceum was investigated as a quantitative trait using restriction fragment length polymorphisms (rflp). two f2 populations of mosquitoes were independently prepared from pairwise matings between a highly susceptible and a refractory strain of a. aegypti. rflp were tested for association with oocyst development on the mosquito midgut. two putative quantitative trait loci (qtl) were identified that significantly ... | 1995 | 7789771 |
| characterization of a salivary gland-specific esterase in the vector mosquito, aedes aegypti. | esterase enzymatic activity was investigated in salivary gland lysates of adult aedes aegypti. esterases in lysates made from female glands had higher specific activity than those in lysates from male glands towards beta-naphthyl acetate but showed no difference with alpha-naphthyl butyrate as a substrate. female salivary gland lysates showed no difference in activity to alpha- and beta-forms of naphthyl acetate and no discernable activity towards alpha-naphthyl caprate. both female and male sal ... | 1995 | 7787844 |
| [deficiency in public services and breeding of aedes aegypti in venezuela]. | 1995 | 7786414 | |
| breeding of aedes aegypti (l.) and aedes albopictus (skuse) in urban housing of sibu town, sarawak. | an aedes survey using various larval survey methods was conducted in 12 urban housing areas and 29 vacant lands in sibu town proper. aedes albopictus larvae were found in all areas surveyed while aedes aegypti larvae were present in 10 localities and 4 vacant lands. there were no significant difference in the house index, breteau and larval density index of these two aedes (stegomyia) species from the survey areas. the proportion of containers positive with ae. aegypti and ae. albopictus in area ... | 1994 | 7777923 |
| effect of sublethal dosages of insecticides on chikungunya virus susceptible and refractory strains of aedes aegypti. | three strains of aedes aegypti mosquitos viz (i) crs, refractory to chikungunya (chik) virus by oral route of infection but susceptible to ddt (2) css, susceptible to chik virus and also susceptible to ddt (3) css-ddtr, susceptible to chik virus but resistant to ddt, were examined for the effect of sublethal dosages of ddt and deltamethrin on their fecundity. biochemical analysis showed that there was an increase in glutathione s-transferase activity in the css-ddtr strain which was associated w ... | 1994 | 7777922 |
| transient expression of a promoter-reporter construct in differentiated adult salivary glands and embryos of the mosquito aedes aegypti. | vector-borne pathogens develop in close association with specific tissues in their insect hosts. efforts are being made to characterize insect genes that are expressed in tissues that have important roles in pathogen propagation. successful transfection and expression of exogenous genes in terminally differentiated tissues of insects has previously proven difficult. here we report a method that should allow the analysis of genes that are expressed in adult tissues and organs. transient expressio ... | 1995 | 7771613 |
| antibacterial peptides in insect vectors of tropical parasitic disease. | the induction and characterization of immune peptides in two groups of medically important insects, the mosquitoes and blackflies, is currently an important research area. mosquitoes transmit a variety of viral and parasitic diseases including yellow fever, dengue, malaria and lymphatic filariasis. simuliid black flies are vectors of river blindness. the diseases are together responsible for death and morbidity in millions of people each year. the relationship between inducible peptides and bact ... | 1994 | 7768149 |
| isolation of antifungal and larvicidal constituents of diplolophium buchanani by centrifugal partition chromatography. | three phenylpropanoids, myristicin [1], elemicin [2], and trans-isoelemicin [3], together with two furanocoumarins, oxypeucedanin [4] and oxypeucedanin hydrate [5], have been isolated from the leaves of diplolophium buchanani by a separation strategy involving the almost exclusive use of centrifugal partition chromatography. all five compounds were antifungal in a tlc bioautography test using cladosporium cucumerinum. compounds 1-4 exhibited larvicidal activity against aedes aegypti. | 1995 | 7760070 |
| dengue serologic survey in ribeirão preto, são paulo, brazil. | the city of ribeirão preto suffered a dengue 1 epidemic that began in november 1990 and ended in march 1991. a serologic survey designed to detect igg antibodies to the four dengue serotypes and other flaviviruses was carried out in ribeirão preto during september and october of 1992. dengue 1 antibodies were detected in 5.4% of the survey participants. significantly higher seropositivity (9.3%) was found among subjects residing in the northwest sector of ribeirão preto than among those living i ... | 1995 | 7757124 |
| mosquito larvicidal activity of escherichia coli with combinations of genes from bacillus thuringiensis subsp. israelensis. | the genes cryiva and cryivd, encoding 134- and 72-kda proteins, respectively, and the gene for a regulatory 20-kda polypeptide of bacillus thuringiensis subsp. israelensis (serovar h14) were cloned in all seven possible combinations by the escherichia coli expression vectors pt7 and puhe. the four combinations containing cryiva (cryiva alone, with cryivd, with the 20-kda-protein gene, and with both) displayed high levels of mosquito larvicidal activity in puhe. the toxicity of the combination of ... | 1995 | 7751296 |
| comparison of migration and encapsulation of brugia malayi microfilariae from the midgut to the hemocoel between anopheles quadrimaculatus and aedes aegypti. | comparisons were made of migration and encapsulation of ingested sheathed microfilariae of brugia malayi from the midgut into the hemocoel between anopheles quadrimaculatus (refractory and susceptible strains to b. malayi) and aedes aegypti (black-eyed, liverpool strain susceptible to b. malayi). encapsulation and melanization of microfilarial sheaths and microfilariae occurred in both strains of an. quadrimaculatus and in ae. aegypti. in both strains of an. quadrimaculatus, by 4 hr and by 24 hr ... | 1995 | 7745283 |
| comparative linkage maps for the mosquitoes, aedes albopictus and ae. aegypti, based on common rflp loci. | aedes albopictus and aedes aegypti are members of the mosquito family culicidae and share a haploid chromosome complement of three. although a genetic linkage map based on restriction fragment length polymorphism (rflp), markers exists for ae. aegypti, the extent of synteny and linkage order conservation between the two species was unknown. a comparative linkage map for ae. albopictus was constructed based mainly on cdna clones from ae. aegypti. nearly all ae. aegypti probes hybridized to ae. al ... | 1995 | 7742975 |
| indirect control of yolk protein genes by 20-hydroxyecdysone in the fat body of the mosquito, aedes aegypti. | in response to a blood meal, the fat body of the female mosquito, aedes aegypti, begins massive production of several yolk proteins which are subsequently stored in the developing oocytes. although 20-hydroxyecdysone (20e) is important for initiation and maintenance of expression of the yolk protein genes encoding vitellogenin (vg) and vitellogenic carboxypeptidase (vcp), the exact nature of 20e action has not been clearly defined. a primary question is whether this hormone directly stimulates e ... | 1995 | 7742832 |
| brugia pahangi: the effects of cecropins on microfilariae in vitro and in aedes aegypti. | synthetic cecropins, antibacterial peptides from insect haemolymph, have been tested for their ability to attenuate the motility of microfilariae of the filarial nematode brugia pahangi in an in vitro assay. fifty micromolar concentrations of these peptides, equivalent to physiological concentrations in immune-stimulated insects, cause significant attenuation of motility compared with untreated microfilariae. similar results were obtained with cecropins a and b. this is the lowest concentration ... | 1995 | 7729475 |
| [aedes albopictus and aedes aegypti at ile de la réunion]. | aedes albopictus and aedes aegypti are both found on the island. the former, being the probable vector of dengue on the island, is highly anthropophilic. it breeds in domestic environments as well as in natural breeding sites. the latter has a more restricted distribution and is never anthropophilic. larvae of ae. aegypti are only found in natural habitats, always associated with ae. albopictus, but in small proportions. such an ecology and behaviour for the typicus form of ae. aegypti are abnor ... | 1994 | 7726666 |
| mosquito bite anaphylaxis: immunotherapy with whole body extracts. | adverse reactions to mosquito bites have been recognized for some time. these usually consist of large local swellings and redness, generalized urticaria, angioedema and less easily definable responses such as nausea, dizziness, headaches, and lethargy. | 1995 | 7719881 |
| early trypsin activity is part of the signal transduction system that activates transcription of the late trypsin gene in the midgut of the mosquito, aedes aegypti. | trypsin activity during the first hours after feeding is essential to induce late trypsin gene expression. these results are consistent with the idea that free amino acids or other products released during digestion might be the initial signal for transcriptional activation of late trypsin. besides early trypsin, some other factor(s) have to be translated for induction of late trypsin. this is the first case in which the proteolytic activity of a digestive enzyme is part of the signal transducti ... | 1995 | 7711754 |
| mosquito ecdysteroid receptor: analysis of the cdna and expression during vitellogenesis. | an insect steroid hormone, 20-hydroxyecdysone (20e), plays an important role in regulating egg maturation in mosquitoes. to better understand its role, we cloned the cdna coding for the putative ecdysteroid receptor from the mosquito, aedes aegypti (aaecr). the 4158 bp aaecr cdna has an open reading frame of 675 amino acids with 10 potential glycosylation sites and a putative phosphorylation polyserine domain. the aaecr has a dna binding domain with two zinc fingers and a ligand binding domain c ... | 1995 | 7711747 |
| the effect of multiple host contacts on the infectivity of dengue-2 virus-infected aedes aegypti. | this study was designed to determine if transmission rates for dengue-2 virus by aedes aegypti are altered by mosquitos probing a host for blood or imbibing blood prior to attempting transmission. aedes aegypti is known to contact multiple hosts during each egg-laying cycle and multiple host contacts might diminish the amount of virus in infected mosquito's salivary glands or ducts and render them functionally uninfective. probing a host 5, 10, or 20 consecutive times did not significantly alter ... | 1995 | 7707190 |
| aedes albopictus in muzquiz city, coahuila, mexico. | aedes albopictus was found in m. muzquiz, coahuila, mexico, in the summer of 1993. aedes albopictus larvae were collected in tires and bottles at low density. aedes aegypti, toxorhynchites theobaldi, culex quinquefasciatus, and culiseta incidens were collected in the same containers. | 1994 | 7707068 |